mohammed hasouni

Aims: The aims of the current study were to show the differences and similarities between original Platelet-Rich Fibrin (PRF) clots produced by a specifically designed centrifuge for this purpose and those clots produced by other centrifuges using bright light microscopy. Materials and Methods: The study included five human volunteers. From each volunteer, six 9 ml blood samples were collected (Total number= 30) and immediately centrifuged, each ten blood samples allocated to a centrifuge group (total of three), one of which was the original recommended centrifuge (Hettich). A set of six histological observations were made and considered as a histological scoring system for the purpose of comparison of different membranes. Results: Out of the six observations, only two showed a significant difference between the three membranes produced namely density of inner fibrin zone density and cell border morphology. Conclusions: Differences in clot membrane histological observations are due ...

Aims: The aims of this study is to evaluate the effect of chitosan prepared from Iraqi freshwater crabs and marine shrimps in accelerating bone healing in surgically created bone defects in sheep. Materials and Methods: Five healthy male sheep were included in the experiment. Each sheep model was to serve as four observation subgroups where each limb in the same sheep represented a specific observation period and according to a preplanned sacrifice schedule. In each limb, four monocortical defects were created, three filled with three chitosan samples of different sources and one defect left empty (negative control). Chitosan tissue responses in addition to its effect on accelerating bone healing were evaluated radio graphically and histopathologically at two, four, six and eight weeks postsurgical intervals. Results: In this study, radio graphically there was a trend of greater radiopacity (represented by increase in mean gray scale) in all chitosan filled bone defects when compari...

Aims: To evaluate the effect of medical chitosan on wound healing. Materials and Methods: An experimental open skin wounds were made on the dorsal side of fifteen domestic rabbits. A 0.04gm of medical chitosan (degree of acetylation=90.0%) was applied. Each individual animal given intramuscular dose of kitamin hydrochloride (anesthetic and analgesic) 4mg/kg and xylazin base (anesthetic and muscle relaxant) 5mg/kg injected into rabbit’s thigh muscle. After 10-15 minutes, anesthetic integrity was checked by testing loss of ear pinch reflex. Four skin incisions were made in the dorsal site of the animal skin. Each incision was about 1cm in length. These incisions were divided into 4 groups according to placement of the material and suturing (Ca: control incision left without suturing or placement of material, Cs: control suturing of the incision only, Cha: chitosan placement only, Chs: chitosan placement and suturing of the incision). Post operatively single dose of 5mg/kg oxytetracycl...

Aim: The aims of this study are to prepare chitosan from Iraqi freshwater crabs and marine shrimps in addition to estimation of its physicochemical properties. Materials and Methods: The procedure of chitosan isolation involved the following steps: Demineralization step: Baths of 10% acetic acid solutions mixed with 1% hydrochloric acid (HCl) solutions were used for removal of calcium carbonate from grounded shell at ambient temperature with a solution to solid ratio of 10ml/g. Deproteinization step: This process was achieved by using reflux apparatus for refluxing of demineralized samples in 4% sodium hydroxide solutions at 100C with a solution to solid ratio of 10 ml/g. Deacetylation of chitin to chitosan: The isolated chitin powder was deacetylated by treatment with 50% NaOH solution under autoclaving conditions (15 psi/121 °C) for 30 minuteswith a solution to solid ratio of 10 ml/g and finally the Viscosity-average molecular weight of chitosan, Degree of De-acetylation (DDA), Pe...

Objective: Radio graphical evaluation of healing after local administration of salmon calcitonin of surgically created bone defects in tibia of sheep model. Study design: Healing of bone defect still represent a challenge to health professional in several areas. In this article, the effect of salmon calcitonin on bone repair was studied.. Differences between drill holes, which filled with new bone were taken and represented as increase radio density. Seven sheep were divided into control ( n=3) and experimental group (n=4). Uniform 3−4 surgical defects on the tibia were created. In the experimental group the drill holes was filled with salmon Calcitonin 10 IU/ cavity carried by gelatin sponge, while in control group one hole filled with gelatin sponge to serve as control positive and the other holes left empty to serve as negative control. All animals killed after (2, 4, 8, 12 weeks) postoperatively. The amount of new bone formation was evaluated radio graphically. A radiographic ex...