K. Hadziavdic
University of Bergen, Department of Biology, Graduate Student
Research Interests: Principal Component Analysis, Cell Signaling, Signal Transduction, Erythropoietin, Single cell analysis, and 10 moreAcute Myeloid Leukemia, Signal Transduction Pathway Models, Valproic Acid, Unsupervised Clustering, Histone deacetylase inhibitor, Combination drug therapy, Targeted Therapy, Growth Factor, Intracellular Signaling, and Blood Cancer
High throughput sequencing technology has great promise for biodiversity studies. However, an underlying assumption is that the primers used in these studies are universal for the prokaryotic or eukaryotic groups of interest. Full primer... more
High throughput sequencing technology has great promise for biodiversity studies. However, an underlying assumption is that the primers used in these studies are universal for the prokaryotic or eukaryotic groups of interest. Full primer universality is difficult or impossible to achieve and studies using different primer sets make biodiversity comparisons problematic. The aim of this study was to design and optimize universal eukaryotic primers that could be used as a standard in future biodiversity studies. Using the alignment of all eukaryotic sequences from the publicly available SILVA database, we generated a full characterization of variable versus conserved regions in the 18S rRNA gene. All variable regions within this gene were analyzed and our results suggested that the V2, V4 and V9 regions were best suited for biodiversity assessments. Previously published universal eukaryotic primers as well as a number of self-designed primers were mapped to the alignment. Primer select...