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A targeted amplicon-based metagenomics approach (metabarcoding) provides detailed access to the diversity of the mycobiome in any substrate in distinct environments on Earth. Fungi are the main decomposers of lignocellulosic woody debris in terrestrial forested ecosystems, contributing significantly to the global carbon cycle. The main objectives of this study were to assess the fungal taxonomic diversity in fallen woody debris samples from two Neotropical forest fragments (rainforest and seasonal forest), to analyze the qualitative and quantitative components of the taxonomic diversity, and to investigate the functional diversity of the ecological groups detected. Our study comprised three main methodological steps: (i) sampling in the field; (ii) extraction of DNA, amplification of targeted segments and massively parallel sequencing; and (iii) data analysis and interpretation. A total of 110 molecular operational taxonomic units showing sequence similarity of 95% or more across th...

In early 2015, a ZIKA Virus (ZIKV) infection outbreak was recognized in northeast Brazil, where concerns over its possible links with infant microcephaly have been discussed. Providing a causal link between ZIKV infection and birth defects is still a challenge. MicroRNAs (miRNAs) are small noncoding RNAs (sncRNAs) that regulate post-transcriptional gene expression by translational repression, and play important roles in viral pathogenesis and brain development. The potential for flavivirus-mediated miRNA signalling dysfunction in brain-tissue development provides a compelling hypothesis to test the perceived link between ZIKV and microcephaly. Here, we applied in silico analyses to provide novel insights to understand how Congenital ZIKA Syndrome symptoms may be related to an imbalance in miRNAs function. Moreover, following World Health Organization (WHO) recommendations, we have assembled a database to help target investigations of the possible relationship between ZIKV symptoms and miRNA-mediated human gene expression. We have computationally predicted both miRNAs encoded by ZIKV able to target genes in the human genome and cellular (human) miRNAs capable of interacting with ZIKV genomes. Our results represent a step forward in the ZIKV studies, providing new insights to support research in this field and identify potential targets for therapy.

Here, we present the draft genome sequence of the type strain of "Acidibacillus ferrooxidans," a mesophilic, heterotrophic, and acidophilic bacterium that was isolated from mine spoilage subjected to accelerated weathering in humidity cell tests carried out by the former U.S. Bureau of Mines in Salt Lake City, UT.

In early 2015, a ZIKA Virus (ZIKV) infection outbreak was recognized in northeast Brazil, where concerns over its possible links with infant microcephaly have been discussed. Providing a definitive link between ZIKV infection and birth defects is still a big challenge. MicroRNAs (miRNAs), are small noncoding RNAs that regulating post-transcriptional gene expression by translational repression, and play important roles in viral pathogenesis and brain development. The potential for flavivirus-mediated miRNA signaling dysfunction in brain-tissue develop provides a compelling mechanism underlying perceived linked between ZIKV and microcephaly. Here, we provide novel evidences toward to understand the mechanism in which miRNAs can be linked to the congenital ZIKA syndrome symptoms. Moreover, following World Health Organization (WHO) recommendations, we have assembled a database to help target mechanistic investigations of this possible relationship between ZIKV symptoms and miRNA mediate...

Here, we report the draft genome sequence of "Acidibacillus ferrooxidans" strain ITV01, a ferrous iron- and sulfide-mineral-oxidizing, obligate heterotrophic, and acidophilic bacterium affiliated with the phylum Firmicutes. Strain ITV01 was isolated from neutral drainage from a low-grade chalcopyrite from a mine in northern Brazil.

Bees are manufacturers of relevant economical products and have a pollinator role fundamental to ecosystems. Traditionally, studies focused on the genusMeliponahave been mostly based on behavioral, and social organization and ecological aspects. Only recently the evolutionary history of this genus has been assessed using molecular markers, including mitochondrial genes. Even though these studies have shed light on the evolutionary history of theMeliponagenus, a more accurate picture may emerge when full nuclear and mitochondrial genomes ofMeliponaspecies become available. Here we present the assembly, annotation, and characterization of a draft mitochondrial genome of the Brazilian stingless beeMelipona scutellarisusingMelipona bicoloras a reference organism. Using Illumina MiSeq data, we achieved the annotation of all protein coding genes, as well as the genes for the two ribosomal subunits (16S and 12S) and transfer RNA genes as well. Using the COI sequence as a DNA barcode, we fo...

Journal of Engineering Science and Technology Vol. 4, No. 3 (2009) 328 - 343 © School of Engineering, Taylor's University College ... SOLAR WATER DISINFECTION IN NORTHEAST BRAZIL: KINETICS OF THE PROCESS AND THE STUDY FOR THE DEVELOPMENT OF ...