Hymenolepis nana es un endo-parásito encontrado en humanos y en roedores, y la infección se adquiere mediante la ingestión de agua o de alimentos contaminados con huevos del parásito. La morfología del parásito en ambos huéspedes sugiere... more
Hymenolepis nana es un endo-parásito encontrado en humanos y en roedores, y la infección se adquiere mediante la ingestión de agua o de alimentos contaminados con huevos del parásito. La morfología del parásito en ambos huéspedes sugiere una posible zoonosis. Debido al fracaso para infectar ratones con huevos del parásito aislado de heces humanas, se cree que existe un complejo de especies adaptadas para cada huésped. El objetivo fue analizar las secuencias genómicas ITS2 ribosómicas y COX1 mitocondrial del parásito. Materiales y métodos. Mediante el copropara-sitoscópico de Faust se analizaron 129 muestras deheces de niños y 20 muestras de heces de ratones. Se aislaron dos gusanos adultos de las heces humanas y 10 adultos del intestino de ratones. El ADN de los parásitos se purificó y empleó como molde para la amplificación por PCR de ITS-2 y COX-1. Los amplicones fueron secuenciados, editados y comparados con secuencias del GenBank utilizando los programas Bioedit y CLC Sequence. ...
Gnathostomiasis was first described in Mexico in 1970, and endemic areas have been spreading in six states of this country. In Culiacan, Sinaloa, 300 cases of cutaneous larva migrans were recorded between January 1992 and December 1995.... more
Gnathostomiasis was first described in Mexico in 1970, and endemic areas have been spreading in six states of this country. In Culiacan, Sinaloa, 300 cases of cutaneous larva migrans were recorded between January 1992 and December 1995. In addition, a Gnathostoma larva was surgically removed from the eye of one patient. Cutaneous lesions were observed mainly on the face, neck, arms, and legs. About 70% of the patients showed eosinophilia. A skin biopsy was carried out on 35 patients and the parasite was identified in histopathologic sections of 12 of these patients. In four patients, the larva migrated out spontaneously from the skin. An enzyme-linked immunosorbent assay using a crude somatic extract of adult Gnathostoma doloresi worms showed that 93% of the patients were seropositive, confirming the reliability of clinical diagnosis. A total of 14 advanced third-stage Gnathostoma larvae were found in four species of ichthyophagous birds captured on dams and dikes near the city of C...
Research Interests:
The hypoglycemic activity of olive leaf was studied. Maximum hypoglycemic activity was obtained from samples collected in the winter months, especially in February. One of the compounds responsible for this activity was oleuropeoside,... more
The hypoglycemic activity of olive leaf was studied. Maximum hypoglycemic activity was obtained from samples collected in the winter months, especially in February. One of the compounds responsible for this activity was oleuropeoside, which showed activity at a dose of 16 mg/kg. This compound also demonstrated antidiabetic activity in animals with alloxan-induced diabetes. The hypoglycemic activity of this compound may result from two mechanisms: (a) potentiation of glucose-induced insulin release, and (b) increased peripheral uptake of glucose.
Research Interests:
We have investigated the antidiabetic activity of luteolin 5-rutinoside in streptozotocin(STZ)-induced diabetic rats. Treatment for 20 days with 2 mg/kg increased both pancreatic insulin and DMA content. When both luteolin 5-rutinoside (2... more
We have investigated the antidiabetic activity of luteolin 5-rutinoside in streptozotocin(STZ)-induced diabetic rats. Treatment for 20 days with 2 mg/kg increased both pancreatic insulin and DMA content. When both luteolin 5-rutinoside (2 mg/kg) and glibenclamide (1 mg/kg) were administered concurrently to STZ-diabetic rats, a marked antidiabetic activity was achieved. This effect was evidenced by a significant decrease in glycemia levels