Dr.Ketaki Kulkarni

Objective: The emergence of Human immunodeciency virus(HIV), Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections has posed the greatest challenge to public health in modern era. Study was done retrospectively to nd out burden of HIV, HBV and HCV in community. Similarly we tried to study other sociodemographic factors and correlates of HIV. Methodology: A retrospective study was done. Serum samples were processed and analysed in the department of microbiology, in tertiary care hospital since JAN 20 to JAN 22. The study included all OPD and IPD patients sample. Results: A total of 21600 samples were analysed retrospectively. Prior consent was taken. Of these, 13490 were males and 8110 were females. The seroprevalence of HIV was found to be 0.5% , HBsAg 0.70% and HCV was 0.25 among population visiting Tertiary Care Centre. Coinfection rate of hepatitis B and hepatitis C with HIV positive patients was found to be 5 (0.23%). Conclusions: Prevalence HIV, HBV and HCV seropositivity was found to be very low in this population around our tertiary care hospital. Sexually transmitted infection history was most relevant risk factors of co-infection among seropositive population. Coinfection rate of hepatitis B and hepatitis C with HIV positive patients was on higher side among patients with high risk sexual behaviour..

Background- Sexually transmitted infections (STI's) are foremost community well being problems midst the women specially in developing countries. Vaginosis is conjoint gynaecological disorder stated in 5-51 % of women reliant upon demographics & whether or not they are suggestive of symptoms.1 Bacterial vaginosis is presently measured as the furthermost predominant vaginal infection. 50% of the total statistics of BV infections are symptomless. It is related with low birth weight & avoidable preterm birth. The study included 800 Methods- patients attending ANC & STI clinic who were screened for Bacterial vaginosis, candida, Gonorrhoea, Trichomonas vaginalis, HIV, Hepatitis b, Herpes simplex virus –II, Syphilis, Chlamydia by appropriate serological and bacteriological identication methods. In this study vaginal Results- trichomoniasis showed highly signicant association with bacterial vaginosis. In present study candidiasis OR=0.19 (95% CI) 0.19(0.14-0.28)( p= 0.000 ) showed highly signicant association with bacterial vaginosis. Similarly HIV (OR=5.79 (95% CI) (1.09-57.35) p=0.0138 and Syphilis OR=3.66 (95% CI) (1.16-13.57) p = 0.0108 also signicantly associated with bacterial vaginosis. A diagnosis of Chlamydia, Gonorrhoea and HSV-2 showed no signicant association with BV (all p>0.05). Screening for BV could reduce HIV Conclusions- -1 transmision. Additionally, BV is associated with STIs including HIV, so further investigation is needed to apprehend the potential role of screening and treatment of BV in STIs /HIV prevention programs.

Aims: To assess the knowledge of Biomedical waste (BMW) categories, colour coding, transport, storage & disposal of Biomedical waste among the healthcare workers. Study Design: Cross sectional. Place and Duration of Study: Multi speciality Hospital, Pune, over the duration of 1 month. Methodology: A predesigned questionnaire containing closed-ended questions was used to conduct this cross sectional study on HCWs. The data related to awareness & knowledge about various aspects of Biomedical waste amongst the Healthcare workers was collected. Results: Out of total 100 Healthcare workers (HCWs), 40 doctors were correctly knowing all the categories of Biomedical waste. 45 doctors & 40 nursing staff (total 85 out of 100 HCWs) could answer correctly the questions on colour coding of BMW. Only 30 doctors & 21 nursing staff could answer correctly about BMW transport. 25 doctors &18 nurses could answer correctly the questions related to BMW storage & disposal. Conclusion: The vigorous & repe...

BackgroundMethicillin resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community acquired infections. Increased use of vancomycin which his a cornerstone for MRSA treatment has resulted in the emergence of MRSA with reduced susceptibility to vancomycin with high MIC values in susceptible range, phenomenon referred to as MIC creep.[2-4]. Patients infected with such MRSA isolates experience poor clinical outcome, delayed response to therapy, increase relapse rates, increased mortality rates.[8-10]. ObjectivesStudy was undertaken to evaluate local status of Vancomycin MIC in our setup & presence of Vancomycin MIC creep phenomenon among MRSA isolates from clinical specimens MethodsThe prospective study was conducted over a period of 6 months from January 2019 to June 2019. Isolates identified as Staphylococcus aureus using routine bacteriological procedures were labelled as MRSA if they are cefoxitin resistant & further tested for different antimicrobials a...

Introduction: Infections caused by Methicillin Resistant Staphylococcus aureus (MRSA) are associated with increased morbidity, longer antimicrobial therapy, etc. First option for treating invasive MRSA infections is glycopeptide vancomycin. Daptomycin, a lipopeptide rapidly bactericidal invitro against MRSA, is an acceptable alternative. Aim: To identify MRSA isolates from clinical specimens and assess their vancomycin and daptomycin susceptibility pattern. Materials and Methods: The cross-sectional study was conducted over a period of six months (January 2019 to June 2019) on 90 clinical samples in a rural teaching hospital in Pune, Maharashtra, India, including all samples except sputum received in the Microbiology laboratory. MRSA isolates were tested for vancomycin and daptomycin susceptibility by Epsilometer (E) test Minimum Inhibitory Concentration (MIC) method. Results: Among 90 MRSA isolates, most were from pus (51) followed by Urine (23), Blood (9), followed by Miscellaneou...

Introduction : Currently colistin is increasingly being used against multidrug resistant gram negative bacteria which include Pseudomonas aeruginosa,,Acinetobacterbaumanii,Escherichia coli, Klebsiellapneumoniae& Salmonella enterica . [1] Colistin resistant organisms are reported in various parts of world. Methods : Total 200 MDR GNB isolates were included in the study & tested for their susceptibility to colistin by Kirby Bauer Disc Diffusion Test. Results :Isolates consisted of 64 Escherichia coli ,45 Pseudomonas aeruginosa ,44  Klebsiella pneumoniae ,32 Citrobacter koseri ,13 Acinetobacterbaumanii & 2 Proteus mirabilis . Of these, 69 (34.5%) were resistant to Colistin. Colistin resistance in highest number was detected in Escherichia coli isolates obtained from various samples. Conclusion : As colistin resistance is emerging rapidly,measures are necessary to restrict indiscriminate use of antimicrobials& to adhere strictly to the hospital antibiotic policy. it is also the need of ...

Background : Bacterial resistance towards antibiotics is a clinical threat because it increases the problem of infectious disease. Currently colistin is increasingly being used against multidrug resistant gram negative bacteria which include Pseudomonas aeruginosa,,Acinetobacterbaumanii, Escherichia coli, Klebsiellapneumoniae& Salmonella enterica . [1]. Combination antibiotic therapy is frequently used to treat severe gram negative infections though controversial &debatable. Methods : Total 200 MDR GNB isolates were included in the study which were further tested for their susceptibility to Colistin&Meropenem by Kirby Bauer Disc Diffusion Test. Results : Of 200 MDR GNB isolates, 69(34.5%) isolates showed resistance to Colistin.Out of 69 Colistin resistant isolates, 55(79.71 %)  were resistant to Meropenem as well.  Conclusion : As multidrug resistance is emerging rapidly,measures are necessary to restrict indiscriminate use of antimicrobials against common infections & adherence to ...

A 25-year-old transgender patient came with complaints of watery discharge, red eye and photophobia in the left eye since 2 days. The patient had a history of wearing colored contact lenses since 4 years and cleaning the lens with tap water. Culture of lenses on Mac Conkey and blood agar yielded Klebsiella pneumoniae and Pseudomonas aeruginosa. Sabouroud's agar showed yeast cells and double-walled cysts of Acanthamoeba species. On further incubation of Sabouroud's agar, the cysts transformed to trophozoites. Parallel results were obtained on tap water agar. The previous therapy of moxifloxacin was changed to local Neosporin application.