Paras Jain
Ranchi University, Department of Botany, Graduate Student
Synthetic seeds and in vitro propagation are the need of the hour, especially for conservation of medicinal plants which are under the threat of extinction due to extensive exploitation. The plumbagin, Plumbago zeylanica L., is one such... more
Synthetic seeds and in vitro propagation are the need of the hour, especially for conservation of medicinal plants which are under the threat of extinction due to extensive exploitation. The plumbagin, Plumbago zeylanica L., is one such highly exploited medicinal plant. Here, we attempted in vitro propagation of its roots by tissue culture and also synthetic seed development towards conservation of this plant. Its leaves were used as the explant. Surface sterilized explants were aseptically cultured on MS medium supplemented with different plant growth hormones. The embryoid callus produced from tissue culture was then used to produce synthetic seed for large scale production of the plants and to reduce the risk of maintenance, storage and transportation of the cultured plants. For the production of synthetic seed, the embryoid callus were chopped aseptically and were encapsulated with sodium alginate and liquid MS medium without CaCl2 supplemented with growth hormones of similar co...
Research Interests:
Achyranthes aspera is a herb in amaranthaceae family, traditionally used in treatment of several diseases (inflammation, diabetes, hypertension, wounds, pain, pneumonia, diarrhea, dysentery, asthma, cough, dropsy, ulcers, piles,... more
Achyranthes aspera is a herb in amaranthaceae family, traditionally used in treatment of several diseases (inflammation, diabetes, hypertension, wounds, pain, pneumonia, diarrhea, dysentery, asthma, cough, dropsy, ulcers, piles, rheumatism, scabies and other skin
diseases. and fever etc). The present study was carried out to investigate the antimicrobial activities of the methanolic extract and to investigate phytochemical profile of aqueous extract of vegetative parts of Achyranthes aspera. The methanol extracts were screened for antifungal activity against four fungi Fusarium oxysporum, Alternaria solani, Athelia rolfsii and Rhizoctonia solani by food poisoning method. The extracts of fresh Achyranthes aspera showed
a significant and remarkable activity against all these four fungal species when compared to standard. The observed IC50 values for these funguses are 43.75µg/ml for Fusarium oxysporum, 27.5µg/ml for Alternaria spp., 19.37µg/ml for Sclerotium rolfsii. And 18.75µg/ml for Rhizoctonia solani. The phytochemical screening of the aqueous extract indicates that the fresh plant contain tannin,
phlobatannin, terpenoid, flavonoid, cardiac glycoside, phenol and alkaloid. Phytochemical screening indicate the absence of saponin, steroid, free anthraquinone, and carbohydrate.
diseases. and fever etc). The present study was carried out to investigate the antimicrobial activities of the methanolic extract and to investigate phytochemical profile of aqueous extract of vegetative parts of Achyranthes aspera. The methanol extracts were screened for antifungal activity against four fungi Fusarium oxysporum, Alternaria solani, Athelia rolfsii and Rhizoctonia solani by food poisoning method. The extracts of fresh Achyranthes aspera showed
a significant and remarkable activity against all these four fungal species when compared to standard. The observed IC50 values for these funguses are 43.75µg/ml for Fusarium oxysporum, 27.5µg/ml for Alternaria spp., 19.37µg/ml for Sclerotium rolfsii. And 18.75µg/ml for Rhizoctonia solani. The phytochemical screening of the aqueous extract indicates that the fresh plant contain tannin,
phlobatannin, terpenoid, flavonoid, cardiac glycoside, phenol and alkaloid. Phytochemical screening indicate the absence of saponin, steroid, free anthraquinone, and carbohydrate.