DR. NAGENDRA K U M A R CHANDRAWANSHI
I am young researcher in the field of life sciences. My core research interests are Medicinal Plant conservation, cultivation, characterization of pharma valued, another is Mushroom Characterization, taxonomy, edibility characterization, pharmaceutical important assay. Now develop to medicinal properties towards drugs against Cancer, Malnutrition, Antidibetic, Antivirus and Antimicrobial etc.
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Keywords: Split gill mushroom, Antidiabetic assay, Extraction solvent, Metabolic disorder.
KEYWORDS: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
world. Pharmaceutically important alkaloid-colchicine, present in its tubers and
seeds and due to overexploitation it becomes vulnerable in the forests. In the
present investigation, in vitro tuber production was carried out for its propagation
and conservation. The plant possesses a very strong apical dominance.
Consequently, any damage to the plant apical meristem is fatal for it which was also
exhibited during in vitro culture. Only apical meristems were able to produce a
single and un-branched shoots and nodal explants were remained dormant even in
the presence of exogenous cytokinin. The in vitro propagation was accomplished
by the microtuber formation technique, in two steps. Maximum number of
microtubers 9.8±0.8 per culture in eight weeks, were produced in vitro on
Murashige and Skoog medium with sucrose (60 g LG1) and in the presence
of 35.5 μM 6-benzyladenine (BA) with citric acid and polyvinyl pyrrolidone-40.
Subsequently the induced microtubers were sub-cultured on to the medium with
lower cytokinin level, 8.88 μM BA. The individual microtubers with shoots were
subjected to a single step rooting and in vitro acclimatization in coco-pit containing
vessels, exhibited 90% survival. In vitro grown tubers contained less percentage of
colchicine than the natural field-grown plant tubers. However, microtubers showed
increased colchicine content, as they grow older.
Key words: Apical dominance release, bud culture, cytokinin, glory lily
Keywords: Green leaves, black leaves, phytochemical constituents, antimicrobial activity.
Keywords: Penicillium, MIC, Antibacterial activity.
Keywords: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
Keywords: Glory lily, In vitro conservation, Medicinal plant, Microtuberization, Plant growth regulator.
Keywords: Split gill mushroom, Antidiabetic assay, Extraction solvent, Metabolic disorder.
KEYWORDS: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
world. Pharmaceutically important alkaloid-colchicine, present in its tubers and
seeds and due to overexploitation it becomes vulnerable in the forests. In the
present investigation, in vitro tuber production was carried out for its propagation
and conservation. The plant possesses a very strong apical dominance.
Consequently, any damage to the plant apical meristem is fatal for it which was also
exhibited during in vitro culture. Only apical meristems were able to produce a
single and un-branched shoots and nodal explants were remained dormant even in
the presence of exogenous cytokinin. The in vitro propagation was accomplished
by the microtuber formation technique, in two steps. Maximum number of
microtubers 9.8±0.8 per culture in eight weeks, were produced in vitro on
Murashige and Skoog medium with sucrose (60 g LG1) and in the presence
of 35.5 μM 6-benzyladenine (BA) with citric acid and polyvinyl pyrrolidone-40.
Subsequently the induced microtubers were sub-cultured on to the medium with
lower cytokinin level, 8.88 μM BA. The individual microtubers with shoots were
subjected to a single step rooting and in vitro acclimatization in coco-pit containing
vessels, exhibited 90% survival. In vitro grown tubers contained less percentage of
colchicine than the natural field-grown plant tubers. However, microtubers showed
increased colchicine content, as they grow older.
Key words: Apical dominance release, bud culture, cytokinin, glory lily
Keywords: Green leaves, black leaves, phytochemical constituents, antimicrobial activity.
Keywords: Penicillium, MIC, Antibacterial activity.
Keywords: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
Keywords: Glory lily, In vitro conservation, Medicinal plant, Microtuberization, Plant growth regulator.