Berg et al., 1976 - Google Patents
Purification of D-amino acid oxidase from Trigonopsis variabilisBerg et al., 1976
View PDF- Document ID
- 355721936548385321
- Author
- Berg C
- Rodden F
- Publication year
- Publication venue
- Analytical Biochemistry
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Snippet
The d-amino acid oxidase in sonicates of Trigonopsis variabilis was purified by precipitating it with acetone and with ammonlum sulfate, removing nucleo-proteins with protamine sulfate, adsorbing impurities with charcoal, and applying gel and ion-exchange chromatography …
- 102000004674 EC 1.4.3.3 0 title abstract description 22
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases Endopeptidases (3.4.21-3.4.25) derived from bacteria
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- Y10S435/8215—Micro-organisms
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- Y10S435/814—Enzyme separation or purification
- Y10S435/815—Enzyme separation or purification by sorption
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