[go: up one dir, main page]

GB2021594A - Glycerol Dehydrogenase - Google Patents

Glycerol Dehydrogenase

Info

Publication number
GB2021594A
GB2021594A GB7917146A GB7917146A GB2021594A GB 2021594 A GB2021594 A GB 2021594A GB 7917146 A GB7917146 A GB 7917146A GB 7917146 A GB7917146 A GB 7917146A GB 2021594 A GB2021594 A GB 2021594A
Authority
GB
United Kingdom
Prior art keywords
assay
glycerol
strains
enzymes
units
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7917146A
Other versions
GB2021594B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BRUTON J
Original Assignee
BRUTON J
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BRUTON J filed Critical BRUTON J
Priority to GB7917146A priority Critical patent/GB2021594B/en
Publication of GB2021594A publication Critical patent/GB2021594A/en
Application granted granted Critical
Publication of GB2021594B publication Critical patent/GB2021594B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Glycerol dehydrogenase enzymes having exceptionally good thermal stability are produced by culturing novel strains of Bacillus stearothermophilus. Procedures for deriving and identifying suitable strains are described. The strains are grown in conventional culture media, preferably containing 0.05 to 4.0%, especially 0.1 to 1.0%, by weight of glycerol or a glycerol analogue at 40-65 DEG C and pH 5 to 8. The enzyme is isolated by conventional cell disruption and separation techniques, and typically has a molecular weight of 240,000+/-30,000, composed of four similar sub-units, and a specific activity of greater than 5 Units per mg protein at 30 DEG C by the modified assay described. They may be stored as aqueous solutions or a freeze dried solids. The enzymes may be used for assay of serum triglycerides by conventional assay methods, by preferably by the nicotinamide adenine dinucleotide spectrophotometric assay at a pH of 7 to 8.8. The pH is preferably controlled by an amine, especially triethanolamine/HCl, buffer.
GB7917146A 1978-05-22 1979-05-17 Glycerol dehydrogenase Expired GB2021594B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB7917146A GB2021594B (en) 1978-05-22 1979-05-17 Glycerol dehydrogenase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB2119478 1978-05-22
GB7917146A GB2021594B (en) 1978-05-22 1979-05-17 Glycerol dehydrogenase

Publications (2)

Publication Number Publication Date
GB2021594A true GB2021594A (en) 1979-12-05
GB2021594B GB2021594B (en) 1982-10-20

Family

ID=26255203

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7917146A Expired GB2021594B (en) 1978-05-22 1979-05-17 Glycerol dehydrogenase

Country Status (1)

Country Link
GB (1) GB2021594B (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0031553A2 (en) * 1979-12-27 1981-07-08 Roche Diagnostics GmbH Process for the production of glycerol dehydrogenase
EP0123511A2 (en) * 1983-04-21 1984-10-31 Amano Pharmaceutical Co., Ltd. Method and composition for determination of glycerol
EP0178113A1 (en) * 1984-10-05 1986-04-16 Unitika Ltd. Reagent for assaying creatine kinase
EP0195625A2 (en) * 1985-03-19 1986-09-24 British Gas Corporation Biochemical assays
US7651837B2 (en) 2001-05-31 2010-01-26 Fuso Pharmaceutical Industries, Ltd. Method for detecting and identifying microorganism causative of infection

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0031553A2 (en) * 1979-12-27 1981-07-08 Roche Diagnostics GmbH Process for the production of glycerol dehydrogenase
EP0031553A3 (en) * 1979-12-27 1981-11-25 Boehringer Mannheim Gmbh Process for the production of glycerol dehydrogenase
US4395489A (en) * 1979-12-27 1983-07-26 Boehringer Mannheim Gmbh Process for the recovery of glycerol dehydrogenase
EP0123511A2 (en) * 1983-04-21 1984-10-31 Amano Pharmaceutical Co., Ltd. Method and composition for determination of glycerol
EP0123511A3 (en) * 1983-04-21 1986-02-12 Amano Pharmaceutical Co., Ltd. Method and composition for determination of glycerol
EP0178113A1 (en) * 1984-10-05 1986-04-16 Unitika Ltd. Reagent for assaying creatine kinase
EP0195625A2 (en) * 1985-03-19 1986-09-24 British Gas Corporation Biochemical assays
EP0195625A3 (en) * 1985-03-19 1986-11-26 British Gas Corporation Biochemical assays
US7651837B2 (en) 2001-05-31 2010-01-26 Fuso Pharmaceutical Industries, Ltd. Method for detecting and identifying microorganism causative of infection

Also Published As

Publication number Publication date
GB2021594B (en) 1982-10-20

Similar Documents

Publication Publication Date Title
US3912588A (en) Creatine amidohydrolase in the conversion of creatinine to creatine
Li et al. Metalloprotease from Bacillus thuringiensis
US4987076A (en) Uricase and a method for the preparation thereof
US4317878A (en) Test composition containing acidic uricase used for quantitative determination of uric acid in sample
US5416019A (en) Rhodococcus microorganisms that produce phenylalanine dehydroganase
Ohshima et al. Screening of thermostable leucine and alanine dehydrogenases in thermophilic Bacillus strains
Jendrossek et al. Three different proteins exhibiting NAD‐dependent acetaldehyde dehydrogenase activity from Alcaligenes eutrophus
US4740465A (en) Heat-resistant sarcosine oxidase N and process for producing the same
Stadtman et al. [84] Aldehyde dehydrogenase from Clostridium kluyveri: CH3CHO+ DPN++ CoA⇆ Ac∼ SCoA+ DPNH+ H+
JPH04135487A (en) Glucose dehydrogenase and production thereof
GB2021594A (en) Glycerol Dehydrogenase
US4342827A (en) Production of enzymes
JPH0665300B2 (en) Fructosyl amino acid oxidase
Cox et al. Oxidative phosphorylation in Escherichia coli K12. An uncoupled mutant with altered membrane structure
US4645739A (en) Process and reagent for the determination of N-carbamoylsarcosine with the use of a new enzyme
US4357425A (en) Process for producing L-amino acid oxidase
EP0206595B1 (en) Thermally stable tryptophanase process for producing the same, and thermally stable tryptophanase-producing microorganism
US5019505A (en) Enzymes having alpha-glycerol-3-phosphate oxidase activity, production and use thereof
JPH0328188B2 (en)
Izumi et al. Crystalline formate dehydrogenase from Candida methanolica
Hasegawa Distribution in organisms and stereospecificity of β-hydroxyisobutyrate dehydrogenase
JPH04365473A (en) Cryptococcus laurenty dsm2762
JPS60244286A (en) Preparation of oxalic acid oxidase
JPS6248380A (en) Production of cephalosporin c acylase
JP3102543B2 (en) Glutamate dehydrogenase and method for producing the same

Legal Events

Date Code Title Description
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19950517