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ZA843062B - Secretion of correctly processed human growth hormone in e.coli and pseudomonas - Google Patents

Secretion of correctly processed human growth hormone in e.coli and pseudomonas

Info

Publication number
ZA843062B
ZA843062B ZA843062A ZA843062A ZA843062B ZA 843062 B ZA843062 B ZA 843062B ZA 843062 A ZA843062 A ZA 843062A ZA 843062 A ZA843062 A ZA 843062A ZA 843062 B ZA843062 B ZA 843062B
Authority
ZA
South Africa
Prior art keywords
pseudomonas
secretion
coli
growth hormone
human growth
Prior art date
Application number
ZA843062A
Other languages
English (en)
Inventor
Gregory Lawrence Gray
Herbert Louis Heyneker
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of ZA843062B publication Critical patent/ZA843062B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/12—Growth hormone, growth factor other than t-cell or b-cell growth factor, and growth hormone releasing factor; related peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/30—Signal or leader sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
ZA843062A 1983-04-25 1984-04-25 Secretion of correctly processed human growth hormone in e.coli and pseudomonas ZA843062B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/488,232 US4755465A (en) 1983-04-25 1983-04-25 Secretion of correctly processed human growth hormone in E. coli and Pseudomonas

Publications (1)

Publication Number Publication Date
ZA843062B true ZA843062B (en) 1984-11-28

Family

ID=23938879

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA843062A ZA843062B (en) 1983-04-25 1984-04-25 Secretion of correctly processed human growth hormone in e.coli and pseudomonas

Country Status (9)

Country Link
US (1) US4755465A (xx)
EP (1) EP0127305A1 (xx)
JP (2) JPS6045595A (xx)
CA (1) CA1267099A (xx)
DK (1) DK204684A (xx)
ES (1) ES8602136A1 (xx)
IL (1) IL71622A (xx)
NZ (1) NZ207924A (xx)
ZA (1) ZA843062B (xx)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK55685A (da) * 1985-02-07 1986-08-08 Nordisk Gentofte Enzym eller enzymkompleks med proteolytisk aktivitet
US5618697A (en) * 1982-12-10 1997-04-08 Novo Nordisk A/S Process for preparing a desired protein
US4859600A (en) * 1983-04-25 1989-08-22 Genentech, Inc. Recombinant procaryotic cell containing correctly processed human growth hormone
IL71991A (en) 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
BG49718A3 (en) * 1983-07-15 1992-01-15 Bio- Technology General Corp Method for preparing of polypeptid with superoxiddismutasne activitty
DE3588249T2 (de) * 1984-08-16 2004-05-06 Savient Pharmaceuticals, Inc. Methode zur Herstellung von menschlichen Wachstumshormonen
IL76360A0 (en) * 1984-09-26 1986-01-31 Takeda Chemical Industries Ltd Mutual separation of proteins
US6534285B1 (en) * 1984-12-24 2003-03-18 Genentech, Inc. Molecularly cloned acquired immunodeficiency syndrome polypeptides and their methods of use
US5853978A (en) * 1985-12-04 1998-12-29 Genentech, Inc. Molecularly cloned acquired immunodeficiency syndrome polypeptides and methods of use
US4861868A (en) 1985-02-22 1989-08-29 Monsanto Company Production of proteins in procaryotes
DK151585D0 (da) * 1985-04-03 1985-04-03 Nordisk Gentofte Dna-sekvens
US4786501A (en) * 1985-07-15 1988-11-22 International Minerals & Chemical Corp. Cylindrical implants for the controlled release of growth hormones
US4870017A (en) * 1985-09-20 1989-09-26 Cetus Corporation Bacterial methionine N-terminal peptidase
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase
US5013662A (en) * 1985-09-20 1991-05-07 Cetus Corporation Bacterial methionine n-terminal peptidase
FR2597114B1 (fr) * 1986-04-10 1994-02-25 Sanofi Procede microbiologique pour l'obtention d'une proteine par culture d'une souche bacterienne mutante
FR2599753B1 (fr) * 1986-06-05 1989-12-08 Sanofi Sa Sequence d'adn composite et son application pour la production de l'hormone de croissance
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano
US5202119A (en) * 1991-06-28 1993-04-13 Genentech, Inc. Method of stimulating immune response
US5744139A (en) * 1991-06-28 1998-04-28 University Of Tennessee Research Corporation Insulin-like growth factor I (IGF-1) induced improvement of depressed T4/T8 ratios
US5317012A (en) * 1991-10-04 1994-05-31 The University Of Tennessee Research Corporation Human growth hormone induced improvement in depressed T4/T8 ratio
JPH06503479A (ja) * 1991-10-16 1994-04-21 オラージェン，　インコーポレイテッド 動物体細胞の粒子−媒介トランスフォーメーション
JPH07118577B2 (ja) * 1992-12-02 1995-12-18 株式会社リコー 電子装置用基板の検査方法
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
DK2270052T3 (en) * 2001-06-26 2018-07-02 Amgen Inc Antibodies to OPGL
JP4580644B2 (ja) * 2001-07-06 2010-11-17 エルジー ライフ サイエンシズ エルティーディー． バチルスリケニフォルミス由来の新規アミノペプチダーゼ、該アミノペプチダーゼをコードする遺伝子、該遺伝子を含む発現ベクター、該発現ベクターによる形質転換体及び該発現ベクターを利用する天然型蛋白質の製造方法
MXPA04007886A (es) 2002-02-13 2004-10-15 Dow Global Technologies Inc Sobre-expresion de genes de extremozimo en pseudomonadas y bacterias estrechamente relacionadas.
ES2343518T3 (es) * 2002-09-09 2010-08-03 Hanall Biopharma Co., Ltd. Polipeptidos interferon alfa modificados resistentes a proteasas.
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
WO2004099244A2 (en) * 2003-05-09 2004-11-18 Pharmexa A/S Immunogenic human tnf alpha analogues with reduced cytotoxicity and methods of their preparation
WO2005012531A2 (en) * 2003-08-01 2005-02-10 Genentech, Inc. Antibody cdr polypeptide sequences with restricted diversity
MXPA06005634A (es) * 2003-11-19 2006-08-23 Dow Global Technologies Inc Sistemas mejorados de expresion de proteinas.
WO2005089093A2 (en) 2003-11-21 2005-09-29 Dow Global Technologies Inc. Improved expression systems with sec-system secretion
MXPA06006221A (es) * 2003-12-01 2008-02-13 Dow Global Technologies Inc Produccion de particula tipo virus icosaedrico recombinante en organismos del genero pseudomonas.
EP2434016B1 (en) 2004-01-16 2018-11-07 Pfenex Inc. Expression of mammalian proteins in pseudomonas fluorescens
GB2426521A (en) * 2004-02-02 2006-11-29 Ambrx Inc Modified human four helical bundle polypeptides and their uses
CN102532321B (zh) * 2004-06-18 2018-10-12 Ambrx公司 新颖抗原结合多肽和其用途
JP2008503233A (ja) * 2004-06-23 2008-02-07 ユーエスブイ・リミテッド 胎盤および下垂体アイソフォームに由来するキメラヒト成長ホルモンおよび該キメラを得るための方法
CA2569381A1 (en) * 2004-07-21 2006-08-31 Ambrx, Inc. Biosynthetic polypeptides utilizing non-naturally encoded amino acids
BRPI0513826A2 (pt) 2004-07-26 2010-06-22 Dow Global Technologies Inc processo para expressão de proteìna melhorada através de engenharia de cepa
US7998930B2 (en) 2004-11-04 2011-08-16 Hanall Biopharma Co., Ltd. Modified growth hormones
KR101252835B1 (ko) * 2004-12-22 2013-04-10 암브룩스, 인코포레이티드 아미노아실-ｔＲＮＡ 합성효소의 조성물 및 그것의 용도
US7939496B2 (en) 2004-12-22 2011-05-10 Ambrx, Inc. Modified human growth horomone polypeptides and their uses
US8080391B2 (en) 2004-12-22 2011-12-20 Ambrx, Inc. Process of producing non-naturally encoded amino acid containing high conjugated to a water soluble polymer
AU2005322019B2 (en) * 2004-12-22 2010-08-26 Ambrx, Inc. Formulations of human growth hormone comprising a non-naturally encoded amino acid
KR20080026135A (ko) * 2005-06-03 2008-03-24 암브룩스, 인코포레이티드 개선된 인간 인터페론 분자 및 이의 용도
NZ565294A (en) 2005-08-18 2010-06-25 Ambrx Inc Compositions of tRNA and uses thereof
EP2465870A1 (en) 2005-11-07 2012-06-20 Genentech, Inc. Binding polypeptides with diversified and consensus VH/VL hypervariable sequences
PT1954710E (pt) * 2005-11-08 2011-06-07 Ambrx Inc Aceleradores para a modificação de aminoácidos nãonaturais e polipeptídeos de aminoácidos não-naturais
WO2007059312A2 (en) * 2005-11-16 2007-05-24 Ambrx, Inc. Methods and compositions comprising non-natural amino acids
AU2006338198B2 (en) * 2005-12-02 2012-04-26 Genentech, Inc. Binding polypeptides and uses thereof
EP1973951A2 (en) * 2005-12-02 2008-10-01 Genentech, Inc. Binding polypeptides with restricted diversity sequences
CN101448512B (zh) * 2005-12-14 2015-11-25 Ambrx公司 含有非天然氨基酸和多肽的组合物、涉及非天然氨基酸和多肽的方法以及非天然氨基酸和多肽的用途
AU2007292892B9 (en) * 2006-09-08 2013-02-28 Ambrx, Inc. Hybrid suppressor tRNA for vertebrate cells
ES2465473T3 (es) * 2006-09-08 2014-06-05 Ambrx, Inc. Transcripción de arnt supresor en células de vertebrado
EP2615108B1 (en) 2006-09-08 2016-10-26 Ambrx, Inc. Modified human plasma polypeptide or fc scaffolds and thier uses
BRPI0807834A2 (pt) 2007-01-31 2014-07-22 Dow Global Technologies Inc " sequências-líder bacterianas para expressão aumentada ".
KR20140012199A (ko) 2007-03-30 2014-01-29 암브룩스, 인코포레이티드 변형된 ｆｇｆ-21 폴리펩티드 및 그 용도
US20090093019A1 (en) * 2007-04-27 2009-04-09 Phelps Jamie P Production and in vivo assembly of soluble recombinant icosahedral virus-like particles
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
BRPI0810120A2 (pt) 2007-04-27 2014-11-11 Dow Global Technologies Inc Processo para selecionar rapidamente hospedeiros microbianos para a identificação de certas cepas com maior rendimento e/ou qualidade na expressão de proteínas heterólogas
CA2685596A1 (en) * 2007-05-02 2008-11-13 Ambrx, Inc. Modified interferon beta polypeptides and their uses
DK2217265T3 (en) * 2007-11-20 2017-07-03 Ambrx Inc Modified insulin polypeptides and their use
CN103694337B (zh) 2008-02-08 2016-03-02 Ambrx公司 经修饰瘦素多肽和其用途
EP2285965A1 (en) * 2008-03-31 2011-02-23 Pfenex Inc A design for rapidly cloning one or more polypeptide chains into an expression system
WO2010011735A2 (en) * 2008-07-23 2010-01-28 Ambrx, Inc. Modified bovine g-csf polypeptides and their uses
JP5694171B2 (ja) * 2008-09-26 2015-04-01 アンブルックス, インコーポレイテッドAmbrx, Inc. 修飾されている動物のエリスロポエチンポリペプチドおよびそれらの使用
AU2009296267B2 (en) 2008-09-26 2013-10-31 Ambrx, Inc. Non-natural amino acid replication-dependent microorganisms and vaccines
CA2761578C (en) 2009-05-11 2019-06-18 Pfenex, Inc. Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
RU2573909C2 (ru) 2010-03-04 2016-01-27 Пфенекс Инк. Способ получения растворимого рекомбинантного белка интерферона без денатурирования
JP5839411B2 (ja) 2010-03-30 2016-01-06 フェニックス インク． 組み換え型毒素タンパク質の高レベルの発現
CA2794740C (en) 2010-04-01 2019-12-31 Pfenex Inc. Methods for g-csf production in a pseudomonas host cell
ES2972902T3 (es) 2010-08-17 2024-06-17 Ambrx Inc Polipéptidos de relaxina modificados y sus usos
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
TWI480288B (zh) 2010-09-23 2015-04-11 Lilly Co Eli 牛顆粒細胞群落刺激因子及其變體之調配物
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
MX2017004947A (es) 2014-10-24 2017-06-29 Bristol Myers Squibb Co Polipeptidos del factor de crecimiento de fibroblastos 2 (fgf-21) modificados y usos de los mismos.
US10280409B2 (en) 2014-11-20 2019-05-07 Biological E Limited Codon optimized polynucleotide for high level expression of CRM197
WO2016089782A1 (en) 2014-12-01 2016-06-09 Pfenex Inc. Fusion partners for peptide production
ES2963839T3 (es) 2017-02-08 2024-04-02 Bristol Myers Squibb Co Polipéptidos de relaxina modificados que comprenden un potenciador farmacocinético y usos de los mismos
KR20200073280A (ko) 2017-10-27 2020-06-23 피페넥스 인크. 주변세포질 단백질 발현을 위한 박테리아 리더 서열
EP3700921A4 (en) 2017-10-27 2021-12-15 Pfenex Inc. PROCESS FOR THE PRODUCTION OF RECOMBINANT ERWINIA ASPARAGINASE
AU2021410080A1 (en) 2020-12-23 2023-06-22 Jazz Pharmaceuticals Ireland Ltd. Methods of purifying charge-shielded fusion proteins
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE52036B1 (en) * 1979-05-24 1987-05-27 Univ California Non-passageable viruses
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
IE52755B1 (en) * 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
GR76959B (xx) * 1981-01-02 1984-09-04 Univ New York State Res Found
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
IE55244B1 (en) * 1982-05-25 1990-07-18 Lilly Co Eli Cloning vectors for expression of exogenous protein
JPS59135892A (ja) * 1983-01-25 1984-08-04 Kunio Yamane シグナルアミノ酸配列をコードするｄｎａ

Also Published As

Publication number Publication date
ES531896A0 (es) 1985-12-01
DK204684A (da) 1984-10-26
DK204684D0 (da) 1984-04-24
CA1267099A (en) 1990-03-27
US4755465A (en) 1988-07-05
NZ207924A (en) 1988-03-30
IL71622A0 (en) 1984-07-31
AU2721984A (en) 1984-11-01
AU586609B2 (en) 1989-07-20
JPS6045595A (ja) 1985-03-12
JPH05252936A (ja) 1993-10-05
EP0127305A1 (en) 1984-12-05
ES8602136A1 (es) 1985-12-01
IL71622A (en) 1990-03-19

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