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WO2023179806A2 - Système et procédé de mesure de substance biochimique, et support de stockage - Google Patents

Système et procédé de mesure de substance biochimique, et support de stockage Download PDF

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Publication number
WO2023179806A2
WO2023179806A2 PCT/CN2023/106207 CN2023106207W WO2023179806A2 WO 2023179806 A2 WO2023179806 A2 WO 2023179806A2 CN 2023106207 W CN2023106207 W CN 2023106207W WO 2023179806 A2 WO2023179806 A2 WO 2023179806A2
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WO
WIPO (PCT)
Prior art keywords
electrode
working electrode
electrode group
biochemical substance
extraction
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Application number
PCT/CN2023/106207
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English (en)
Chinese (zh)
Other versions
WO2023179806A3 (fr
Inventor
杨玲
陈毅豪
杨芷炎
Original Assignee
杭州柔谷科技有限公司
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Publication date
Application filed by 杭州柔谷科技有限公司 filed Critical 杭州柔谷科技有限公司
Priority to PCT/CN2023/106207 priority Critical patent/WO2023179806A2/fr
Publication of WO2023179806A2 publication Critical patent/WO2023179806A2/fr
Publication of WO2023179806A3 publication Critical patent/WO2023179806A3/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue

Definitions

  • This application relates to the technical field of biochemical substance detection, and specifically relates to a biochemical substance detection system, method and storage medium.
  • the normal life activities of the human body are inseparable from the stimulation and regulation of various biochemical substances in the body. Changes in one or several biochemical indicators will affect the normal operation of the human body and even affect life and health.
  • the human body's blood glucose concentration levels and fluctuations are important monitoring and disease management indicators for patients with abnormal glucose metabolism; luteinizing hormone is one of the important indicators for sex hormone detection, and its concentration level can be used to guide diagnosis and efficacy evaluation.
  • this application provides a biochemical substance detection system, method and storage medium.
  • the detection area is electroporated first and then the biochemical substances are extracted. This can improve the transdermal extraction efficiency of biochemical substances and is more suitable for macromolecular substances. extraction and detection.
  • the present application provides a biochemical substance detection system, including a first electrode group, a second electrode group and a processing device, and the processing device is connected to the first electrode group and the second electrode group;
  • the first electrode group includes a first working electrode with a first biosensing layer modified on its surface
  • the second electrode group includes a second working electrode with a second biosensing layer modified on its surface.
  • the sensing layer and the second biosensing layer are used to detect the same or different biochemical substances;
  • the processing equipment is configured for:
  • the biochemical substance is detected through the first working electrode and/or the second working electrode to determine the concentration of the biochemical substance.
  • the first electrode group further includes a first pair of electrodes, a first reference electrode, a first extraction electrode and a first flexible substrate, and the first working electrode, the first pair of electrodes, the first reference electrode The specific electrode and the first extraction electrode are arranged on the first flexible substrate;
  • the second electrode group also includes a second counter electrode, a second reference electrode, a second extraction electrode and a second flexible substrate.
  • the second working electrode, the second counter electrode, the second reference electrode and the second extraction electrode Electrodes are provided on the second flexible substrate;
  • the first extraction electrode and the second extraction electrode are used to apply the pulse voltage and the constant current to the detection area.
  • the first working electrode is a sheet electrode
  • the first pair of electrodes and the first reference electrode are arranged around the periphery of the first working electrode
  • the first extraction electrode surrounds The peripheral arrangement of the first pair of electrodes
  • the second working electrode is a sheet electrode
  • the second pair of electrodes and the second reference electrode surround the outer periphery of the second working electrode
  • the second extraction electrode surrounds the outer periphery of the second pair of electrodes.
  • the first electrode group is provided with a first support ring, and the first support ring is arranged around the periphery of the electrodes in the first electrode group;
  • the second electrode group is provided with a second support ring, and the second support ring is arranged around the periphery of the electrodes in the second electrode group.
  • the processing device when the biochemical substance is detected through the first working electrode and/or the second working electrode, and the concentration of the biochemical substance is determined, the processing device is configured to:
  • Differential pulse voltammetry is used to determine the concentration of the biochemical substance based on the electrochemical signals generated by the first working electrode and/or the second working electrode.
  • the pulse parameters for applying the pulse voltage include pulse voltage, pulse width, period and number of pulses, the range of the pulse voltage is 100V -500V, and the range of the pulse width is 10 ms - 50 ms, the period ranges from 0 s to 20 s, and the number of pulses ranges from 20 to 200.
  • the current parameters used to apply the constant current include a current value and an extraction time.
  • the current value ranges from 200 ⁇ A to 500 ⁇ A, and the extraction time ranges from 1 min to 10 min.
  • the first biosensing layer and the second biosensing layer are used to detect different biochemical substances, and the biochemical substances include luteinizing hormone and follicle-stimulating hormone.
  • This application also provides a biochemical substance detection method, which is applied to the biochemical substance detection system as described above.
  • the method includes:
  • the biochemical substance is detected through the first working electrode and/or the second working electrode to determine the concentration of the biochemical substance.
  • the present application also provides a computer-readable storage medium.
  • a computer program is stored on the computer-readable storage medium.
  • the computer program is executed by a processor, the steps of the biochemical substance detection method as described above are implemented.
  • the system includes a first electrode group, a second electrode group and processing equipment; the first electrode group includes a first working electrode with a first biosensing layer modified on its surface, and a second The electrode group includes a second working electrode with a second biosensing layer modified on its surface; the processing device is configured to: apply a pulse voltage to the detection area through the first electrode group and the second electrode group to perform electroporation processing; An electrode group and a second electrode group apply a constant current to the detection area to extract biochemical substances into the electrolyte in the area where the first working electrode and the second working electrode are located; the biochemical substances are extracted through the first working electrode and/or the second working electrode.
  • Tests are performed to determine the concentration of biochemical substances.
  • the technical solution of this application first electroporates the detection area and then extracts biochemical substances, which can improve the transdermal extraction efficiency of biochemical substances and is more suitable for the extraction and detection of macromolecular substances.
  • Figure 1 is a schematic structural diagram of a biochemical substance detection system according to the first embodiment.
  • Figure 2 is a schematic diagram of the electroporation channel.
  • Figure 3 is a schematic diagram of an experimental device for biochemical substance detection according to the first embodiment.
  • FIG. 4 is a cross-sectional view of the experimental equipment shown in FIG. 3 .
  • Figure 5 is the DPV response curve of the extraction solution using 150V pulse voltage and different extraction times.
  • Figure 6 is the DPV response curve of the extraction solution using 300V pulse voltage and different extraction times.
  • Figure 7 is a schematic diagram of the extraction results of different processing methods.
  • Figure 8 is a schematic flow chart of a biochemical substance detection method according to the second embodiment.
  • A, B or C or "A, B and/or C” means "any of the following: A; B; C; A and B; A and C; B and C; A, B and C” . Exceptions to this definition occur only when the combination of elements, functions, steps, or operations is inherently mutually exclusive in some manner.
  • FIG. 1 is a schematic structural diagram of a biochemical substance detection system according to the first embodiment.
  • the biochemical substance detection system of this embodiment includes a first electrode group 10 , a second electrode group 20 and a processing device 30 .
  • the processing device 30 is connected to the first electrode group 10 and the second electrode group 20 .
  • the first electrode group 10 includes a first working electrode 11 with a first biosensing layer modified on its surface.
  • the second electrode group 20 includes a second working electrode 21 with a second biosensing layer modified on its surface. The first biosensing layer and the second biosensing layer for detecting the same or different biochemical substances.
  • the processing device 30 is configured to: apply a pulse voltage to the detection area through the first electrode group 10 and the second electrode group 20 to perform electroporation treatment on the detection area; A constant current is applied to the detection area so that the biochemical substances are extracted into the electrolyte in the area where the first working electrode 11 and the second working electrode 21 are located; the biochemical substances are detected through the first working electrode 11 and/or the second working electrode 21, Determine the concentration of biochemical substances.
  • the processing equipment 30 is a large or small electrochemical workstation and can provide the pulse voltage required for electroporation.
  • the first electrode group 10 and the second electrode group 20 can be connected to the processing equipment 30 in a plug-in manner.
  • the first working electrode 11 and the second working electrode 21 can be used to apply pulse voltage and constant current to the detection area and detect biochemical substances to simplify the structure of the electrode group.
  • applying pulse voltage and constant current to the detection area can be performed through other electrodes in the first electrode group 10 and the second electrode group 20 to increase the service life of the first working electrode 11 and the second working electrode 21 .
  • the same or different biochemical substances can be detected by modifying the same or different biosensing layers on the first working electrode 11 and the second working electrode 21 .
  • the first biosensing layer and the second biosensing layer are used to detect different macromolecular biochemical substances, and the macromolecular biochemical substances include luteinizing hormone and follicle-stimulating hormone.
  • the macromolecular biochemical substances include luteinizing hormone and follicle-stimulating hormone.
  • luteinizing hormone as an example, by modifying the surface of the first working electrode 11 with a graphene oxide/thionine/gold nanocomposite, luteinizing hormone antibody, and bovine serum albumin as the first biosensing layer, it can be achieved Testing for luteinizing hormone.
  • a constant current is applied to the detection area through the first electrode group 10 and the second electrode group 20, that is, reverse electroosmosis extraction is performed.
  • an electric field is applied to the skin surface to cause Cl - and Na + in the subcutaneous tissue to move toward the positive and negative electrodes respectively under the action of the electric field, forming a DC current channel from the skin surface through the subcutaneous tissue and back to the skin surface.
  • biochemical substances in subcutaneous tissue fluid can be carried to the skin surface for detection.
  • the extraction efficiency of this method is limited, and it is difficult for macromolecular substances to pass through reverse electroosmosis due to their large diameter. extracted to the skin surface.
  • this application before performing reverse electroosmosis extraction, this application first uses appropriate pulse parameters to electroporate the detection area, thereby improving the transdermal extraction efficiency of biochemical substances and making it more suitable for the extraction and detection of macromolecular substances.
  • a dominant voltage gradient across the skin is established by passing through the non-conductive stratum corneum. If the voltage gradient exceeds the barrier breakdown potential, holes will be formed, allowing substances to penetrate Transmitted through the skin barrier, for example, from the subcutaneous to the skin surface through channels such as hair follicles A, keratinocyte gaps B, sweat glands C, and other pores D. Depending on the pulse parameters used, these channels will re-close after a period of time or return to their original state. After forming a reversible channel on the skin surface and then performing reverse electroosmosis extraction, more target substances can be extracted from tissue fluid and blood, effectively increasing the extraction amount of subcutaneous target substances. At the same time, it is also conducive to improving extraction efficiency and extracting large amounts of target substances. Molecular substances, realizing the detection of macromolecular substances.
  • the first electrode group 10 further includes a first pair of electrodes 12 , a first reference electrode 13 , a first extraction electrode 14 and a first flexible substrate (not shown in FIG. 1 ).
  • the first working electrode 11 , the first pair of electrodes 12, the first reference electrode 13 and the first extraction electrode 14 are arranged on the first flexible substrate.
  • the second electrode group 20 also includes a second counter electrode 22 , a second reference electrode 23 , a second extraction electrode 14 and a second flexible substrate (not shown in FIG. 1 ).
  • the second working electrode 21 and the second counter electrode 22 , the second reference electrode 23 and the second extraction electrode 14 are arranged on the second flexible substrate. Wires and interfaces are provided on the first flexible substrate and the second flexible substrate. Each electrode is connected to the interface through a wire.
  • the interface is used to connect the processing device 30.
  • the arrangement of the wires on the flexible substrate includes a zigzag arrangement and a straight line arrangement. , curve arrangement and other arrangement methods.
  • the materials of the first working electrode 11 and the second working electrode 21 are Pt/C, and the materials of the remaining electrodes can be Ag/AgCl.
  • the flexible base can completely fit the body surface and meet the needs of wearable and easy-to-carry testing.
  • the first working electrode 11 is a sheet electrode
  • the first pair of electrodes 12 and the first reference electrode 13 are arranged around the periphery of the first working electrode 11
  • the first extraction electrode 14 surrounds the periphery of the first pair of electrodes 12 Peripheral settings.
  • the part of the first working electrode 11 located in the detection area is in the shape of a disk, and the first counter electrode 12 and the first reference electrode 13 are commonly surrounding the outer periphery of the disk-shaped part of the first working electrode 11 , that is, surrounding In a circumferential area around the disk-shaped portion of the first working electrode 11, a first pair of electrodes 12 is provided in some areas, and a first reference electrode 13 is provided in another area, thereby reducing the size of the electrodes.
  • the first extraction electrode 14 surrounds the outer periphery of the first pair of electrodes 12 , that is, is disposed on the outermost side of other electrodes in the first electrode group 10 .
  • the second working electrode 21 is a sheet electrode
  • the second counter electrode 22 and the second reference electrode 23 surround the outer periphery of the second working electrode 21
  • the second extraction electrode 14 surrounds the outer periphery of the second counter electrode 22 .
  • the electrodes in the first electrode group 10 and the second electrode group 20 are arranged in the same manner, which will not be described again.
  • the counter electrode is used to form a polarization loop with the corresponding working electrode so that current can flow through the working electrode.
  • the reference electrode is used to provide and maintain a fixed reference potential during the detection of biochemical substances.
  • the first extraction electrode 14 and the second extraction electrode 14 are used to apply pulse voltage to the detection area to perform electroporation treatment, and are also used to apply constant current to the detection area to perform reverse electroosmosis extraction.
  • the pulse parameters for applying the pulse voltage include pulse voltage, pulse width, period and number of pulses.
  • the pulse voltage ranges from 100V to 500V
  • the pulse width ranges from 10 ms to 50 ms.
  • the period ranges from 0 s to 20 s
  • the number of pulses ranges from 20 to 200.
  • the current parameters for applying a constant current include a current value and an extraction time. The current value ranges from 200 ⁇ A to 500 ⁇ A.
  • the extraction time ranges from 1 min to 10 min.
  • the extraction efficiency is highest when the pulse voltage is 150 V or 300 V
  • the number of pulses is 100
  • the constant current is 300 ⁇ A
  • the extraction time is 10 minutes.
  • the first electrode group 10 is provided with a first support ring (not shown in FIG. 1 ), which is disposed around the periphery of the electrodes in the first electrode group 10 , that is, located at the first extraction electrode 14 of the periphery.
  • the second electrode group 20 is provided with a second support ring (not shown in FIG. 1 ).
  • the second support ring is arranged around the periphery of the electrodes in the second electrode group 20 , that is, located on the periphery of the second extraction electrode 14 .
  • the first support ring and the second support ring may be annular adhesive tapes with a certain thickness. One side of the adhesive tape is attached to the flexible base, and the other side is used to adhere to the body surface.
  • a storage space for storing electrolyte can be formed between the electrodes and the body surface through the support ring.
  • An electrical path is formed between the electrode and the body surface.
  • the extracted biochemical substances diffuse in the electrolyte to be in the area of the working electrode to achieve detection.
  • the processing device 30 when detecting biochemical substances through the first working electrode 11 and/or the second working electrode 21 and determining the concentration of the biochemical substances, the processing device 30 is configured to:
  • Differential pulse voltammetry is used to determine the concentration of biochemical substances based on the electrochemical signals generated by the first working electrode 11 and/or the second working electrode 21 .
  • differential pulse voltammetry is used to collect electrochemical signals of the first working electrode 11 and the second working electrode 21 through the processing device 30.
  • the changes of the electrochemical signals of the first working electrode 11 and the second working electrode 21 are related to those to be measured.
  • the concentration of biochemical substances in the liquid is proportional. Therefore, the processing device 30 can determine the concentration of the biochemical substances in the liquid to be tested based on changes in the electrochemical signals of the first working electrode 11 and the second working electrode 21 .
  • this application first electroporates the detection area and then extracts biochemical substances, which can improve the transdermal extraction efficiency of biochemical substances and is more suitable for the extraction and detection of macromolecular substances.
  • the scheme of this application is explained below through experimental data.
  • An experimental equipment includes an electrode group 40, a circuit board 41, a resistance contact 42, a base 43, rat skin 45, a sponge 47, an inner container 46, and an outer container 44.
  • the electrode group 40 includes a first electrode group 10 and a second electrode group 20 .
  • the circuit board 41 is connected to the electrode group and is provided with resistance contacts 42 .
  • the resistance contacts 42 are used to connect to the processing equipment 30 , and the base 43 is used to connect to the processing equipment 30 .
  • the circuit board 41 is supported, and the inner container 46 is used to hold a solution for replacing tissue fluid.
  • a sponge 47 is placed in the inner container 46, and then the rat skin 45 used to simulate the body surface is covered at the opening of the inner container 46, and the sponge 47 is used to The rat skin 45 is used for support, and the inner container 46 is received in the outer container 44.
  • the outer container 44 is used to collect the liquid flowing out of the inner container 46 to avoid contaminating the external environment.
  • curve a1 represents the response curve corresponding to mode a under 150V pulse voltage
  • curve b1 represents the response corresponding to mode b under 150V pulse voltage
  • curve c1 represents the response curve corresponding to mode c under 150V pulse voltage
  • curve d1 represents the response curve corresponding to mode d under 150V pulse voltage
  • curve e1 represents the response curve corresponding to mode e under 150V pulse voltage.
  • the electroporation process is performed using a pulse voltage of 300V, the number of pulses is 100, and the set pulse width and period.
  • the above five extraction methods are used to perform reverse electroosmosis extraction to obtain the liquid to be tested. , detect the liquid to be tested, and obtain the DPV response curve shown in Figure 6, where curve a2 represents the response curve corresponding to mode a under 300V pulse voltage, curve b2 represents the response curve corresponding to mode b under 300V pulse voltage, and curve c2 represents 300V The response curve corresponding to mode c under pulse voltage.
  • Curve d2 represents the response curve corresponding to mode d under pulse voltage of 300V.
  • Curve e2 represents the response curve corresponding to mode e under pulse voltage of 300V.
  • Figures 5 and 6 show the test results corresponding to the optimal pulse parameters and current values obtained experimentally, and the results of the remaining pulse parameters and current values are not shown. It can be seen from Figure 5 and Figure 6 that under the same pulse voltage, the extraction amount of biochemical substances increases with the increase of extraction time. Higher pulse voltage can obtain more extraction amount. The holes formed by electroporation have an impact on the extraction amount. Significant impact.
  • Figure 7 is a schematic diagram of the extraction efficiency at different extraction times when 150V pulse voltage is used for electroporation treatment, 300V pulse voltage is used for electroporation treatment, and no electroporation treatment is performed.
  • Table 1 it can be seen that when the extraction time reaches At 10 minutes, the penetration-promoting multiples of 150V pulse voltage and 300V pulse voltage can both reach 8 times, indicating that electroporation combined with reverse electroosmosis can effectively improve the transdermal extraction efficiency of macromolecule substances.
  • FIG 8 is a schematic flow chart of a biochemical substance detection method according to the second embodiment. As shown in Figure 8, this application also provides a biochemical substance detection method, which is applied to the biochemical substance detection system described in the above embodiment. The method includes:
  • Step S1 Apply a pulse voltage to the detection area through the first electrode group and the second electrode group to perform electroporation treatment on the detection area;
  • Step S2 Apply a constant current to the detection area through the first electrode group and the second electrode group, so that the biochemical substances are extracted into the electrolyte in the area where the first working electrode and the second working electrode are located;
  • Step S3 Detect the biochemical substance through the first working electrode and/or the second working electrode to determine the concentration of the biochemical substance.
  • the first electrode group further includes a first pair of electrodes, a first reference electrode, a first extraction electrode and a first flexible substrate, a first working electrode, a first pair of electrodes, a first reference electrode and a first flexible substrate.
  • An extraction electrode is disposed on the first flexible substrate;
  • the second electrode group also includes a second counter electrode, a second reference electrode, a second extraction electrode and a second flexible substrate.
  • the second working electrode, the second counter electrode, the second reference electrode and the second extraction electrode are arranged on the first Two flexible substrates;
  • the method includes applying pulse voltage and constant current to the detection area through the first extraction electrode and the second extraction electrode.
  • step S1 detects the biochemical substance through the first working electrode and/or the second working electrode, and determines the concentration of the biochemical substance, including:
  • Differential pulse voltammetry is used to determine the concentration of biochemical substances based on the electrochemical signals generated by the first working electrode and/or the second working electrode.
  • the pulse parameters for applying the pulse voltage include pulse voltage, pulse width, period and number of pulses.
  • the pulse voltage ranges from 100V to 500V
  • the pulse width ranges from 10 ms to 50 ms
  • the period ranges from 100V to 500V. is 0 s -20 s
  • the number of pulses ranges from 20 to 200.
  • the current parameters used to apply a constant current include a current value and an extraction time.
  • the current value ranges from 200uA to 500uA, and the extraction time ranges from 1 min to 10 min.
  • the first biosensing layer and the second biosensing layer are used to detect different biochemical substances, and the biochemical substances include luteinizing hormone and follicle-stimulating hormone.
  • This application also provides a computer-readable storage medium.
  • a computer program is stored on the computer-readable storage medium.
  • the computer program is executed by a processor, the steps of the biochemical substance detection method described in the above embodiments are implemented.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

La présente demande concerne un système et un procédé de mesure de substance biochimique, et un support de stockage. Le système comprend un premier groupe d'électrodes, un second groupe d'électrodes et un dispositif de traitement, le premier groupe d'électrodes comprenant une première électrode de travail dont une surface est modifiée avec une première couche de détection biologique ; le second groupe d'électrodes comprend une seconde électrode de travail dont une surface est modifiée avec une seconde couche de détection biologique ; et le dispositif de traitement est conçu pour : appliquer des tensions d'impulsion à une zone de mesure au moyen du premier groupe d'électrodes et du second groupe d'électrodes de façon à effectuer un traitement d'électroporation ; appliquer des courants constants à la zone de mesure au moyen du premier groupe d'électrodes et du second groupe d'électrodes de façon à extraire une substance biochimique des électrolytes dans des zones où la première électrode de travail et la seconde électrode de travail sont situées ; et mesurer la substance biochimique au moyen de la première électrode de travail et/ou de la seconde électrode de travail de façon à déterminer la concentration en substance biochimique. Au moyen de la solution technique dans la présente demande, un traitement d'électroporation est d'abord effectué sur une zone de mesure et une substance biochimique est ensuite extraite, de telle sorte que l'efficacité d'extraction transdermique de la substance biochimique peut être améliorée ; et la solution technique est plus appropriée pour l'extraction et la mesure d'une substance macromoléculaire.
PCT/CN2023/106207 2023-07-06 2023-07-06 Système et procédé de mesure de substance biochimique, et support de stockage WO2023179806A2 (fr)

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Publication number Priority date Publication date Assignee Title
ATE131081T1 (de) * 1988-01-21 1995-12-15 Massachusetts Inst Technology Molekültransport durch gewebe mit der verwendung von elektroporation.
US6587705B1 (en) * 1998-03-13 2003-07-01 Lynn Kim Biosensor, iontophoretic sampling system, and methods of use thereof
JP3600158B2 (ja) * 1998-05-13 2004-12-08 シグナス， インコーポレイテッド 生理学的分析物のモニタリング
JP2007195607A (ja) * 2006-01-24 2007-08-09 Transcutaneous Technologies Inc 薬物投与方法および薬物投与装置
CN105445339B (zh) * 2014-07-31 2018-07-06 天津大学 一种柔性差分式阵列电化学葡萄糖传感器及使用方法
EP3403092A4 (fr) * 2016-01-15 2019-08-28 University of Cincinnati Dispositifs et procédés d'électroporation avancés pour accès d'analyte dans des biofluides
CN106175840A (zh) * 2016-07-01 2016-12-07 中国科学院电子学研究所 一种提取组织液的方法
CN116359306A (zh) * 2023-04-05 2023-06-30 天津大学 一种高面积利用率人体血糖传感器及其制备方法

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