WO2023057375A1 - Composition comprenant une souche de mitsuaria noduli - Google Patents
Composition comprenant une souche de mitsuaria noduli Download PDFInfo
- Publication number
- WO2023057375A1 WO2023057375A1 PCT/EP2022/077447 EP2022077447W WO2023057375A1 WO 2023057375 A1 WO2023057375 A1 WO 2023057375A1 EP 2022077447 W EP2022077447 W EP 2022077447W WO 2023057375 A1 WO2023057375 A1 WO 2023057375A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- strain
- noduli
- mitsuaria
- composition
- cncm
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 23
- 241001007686 Mitsuaria noduli Species 0.000 title claims abstract description 19
- 230000008635 plant growth Effects 0.000 claims abstract description 12
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 claims abstract description 7
- 240000002791 Brassica napus Species 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 9
- 208000005156 Dehydration Diseases 0.000 claims description 8
- 240000009088 Fragaria x ananassa Species 0.000 claims description 8
- 240000007926 Ocimum gratissimum Species 0.000 claims description 8
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 claims description 7
- 239000002689 soil Substances 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 244000020551 Helianthus annuus Species 0.000 claims description 4
- 235000010676 Ocimum basilicum Nutrition 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 4
- 230000035764 nutrition Effects 0.000 claims description 4
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 235000021012 strawberries Nutrition 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 5
- 239000002054 inoculum Substances 0.000 description 6
- 102100027603 Fetal and adult testis-expressed transcript protein Human genes 0.000 description 5
- 101000937113 Homo sapiens Fetal and adult testis-expressed transcript protein Proteins 0.000 description 5
- 241000179039 Paenibacillus Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000002361 compost Substances 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 239000000284 extract Substances 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000001863 plant nutrition Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 235000020238 sunflower seed Nutrition 0.000 description 2
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 241001184073 Basilicum Species 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 240000004385 Centaurea cyanus Species 0.000 description 1
- 235000005940 Centaurea cyanus Nutrition 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 244000060011 Cocos nucifera Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- 240000004585 Dactylis glomerata Species 0.000 description 1
- 241000234642 Festuca Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000208818 Helianthus Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- 241000209082 Lolium Species 0.000 description 1
- 240000000894 Lupinus albus Species 0.000 description 1
- 235000010649 Lupinus albus Nutrition 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 241000918624 Mitsuaria Species 0.000 description 1
- 241000219830 Onobrychis Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 235000002096 Vicia faba var. equina Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- -1 chalk Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005489 dwarf bean Nutrition 0.000 description 1
- 244000013123 dwarf bean Species 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000037125 natural defense Effects 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000009369 viticulture Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 241000441614 x Festulolium Species 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
Definitions
- Composition comprising a strain of Mitsuaria noduli
- the present invention relates to the field of agriculture and, more specifically, to the use of a strain belonging to the Mitsuaria noduli species for improving the growth of plants.
- Mitsuaria noduli a particular species of Mitsuaria (i.e. Mitsuaria noduli) exerts markedly superior effects, in terms of plant growth and protection, compared to those obtained with other reference bacterial species.
- the object of the invention is therefore to provide a new composition, of high efficacy, for improving the growth of plants.
- a first object of the invention is aimed at a composition intended to improve the growth of plants comprising at least one strain of bacteria belonging to the species Mitsuaria noduli.
- this strain is the strain of Mitsuaria noduli deposited at the CNCM on July 30, 2021 under number 1-5724 or 1-5725.
- a second object of the invention relates to a method for increasing the growth of plants which comprises a step of application to the seed, the soil, the root or leaf part of the plant by spraying a composition as previously described.
- the method according to the invention aims to increase the growth of plants under water stress.
- this improvement can materialize by:
- a third object of the invention relates to the use of a bacterial strain belonging to the species Mitsuaria noduli as a biostimulant.
- said biostimulant is a biostimulant under water stress.
- this biostimulant aims to:
- a fourth object of the invention relates to the CNCM 1-5724 and CNCM 1-5725 strains
- composition according to the invention typically comprises from 10 4 to 10 12 CFU/g or CFU/ml, in particular from 10 5 to 10 10 CFU/g or CFU/ml, preferably from 10 6 to 10 8 CFU/ g or CFU/ml of Mitsuaria noduli.
- the composition according to the invention may comprise from 0.005 to 4% by weight (relative to the total weight of the composition) of Mitsuaria noduli, preferably from 0.1 to 3 by weight (relative to to the total weight of the composition) of Mitsuaria noduli.
- composition according to the invention may take one of the many forms suitable for use as a biostimulant such as solid forms (eg powders, in particular wettable powders, or granules, in particular micro-granules) , liquids (eg suspensions) or gels.
- solid forms eg powders, in particular wettable powders, or granules, in particular micro-granules
- liquids eg suspensions
- the Mitsuaria noduli strain is advantageously in live form.
- extracts of said strain may be used.
- biostimulant is meant. Also, and within the meaning of the present invention, the term biostimulant includes that of fertilizer.
- composition according to the invention may comprise at least one agriculturally acceptable carrier.
- Such supports include in particular talc, chalk, maltodextrin, glucose, kaolin, diatomaceous earth, chitin, alginates or even carrageenan.
- composition according to the invention may comprise at least one other active agent.
- the composition of the invention is characterized in that it comprises at least said strain of bacteria in combination with other live, inactivated microorganisms (yeasts, bacteria and/or fungi). or in the form of extracts.
- the composition will additionally comprise, and advantageously, microorganisms, live or inactivated, extracts of microorganisms, fertilizers, herbicides, insecticides, fungicides, mineral solutions and/or or growing media.
- the invention also relates to the formulations of the composition for treatment on the ground, treatment of the root part, foliar treatment and/or treatment of the seed.
- composition according to the invention may be applied repeatedly, for example every 3 to 6 weeks, preferably on the seed or at the time of application. emergence, in the first stages of flowering, appear favorable to the development of the plant and increase its growth.
- composition defined above to cereal crops (wheat, barley, oats, rye), to root crops (sugar beet, potato, corn), to legumes (alfalfa, clover, sainfoin) , grasses (ryegrass, fescue, orchardgrass festulolium), oilseed crops (soya, rapeseed, cornflower, field bean, white lupin), vegetable and market garden crops, fruit growing, viticulture and ornamental crops - mental (floral production, seedling nurseries) are of particular interest.
- the strawberry plants used were of the ciflorette variety, which were planted in 9 trays comprising 4.3 kg of THREL (FLORANTAISE) compost.
- An inoculum comprising the Mistuaria noduli Bio T025 strain (CNCM-° I-5724) or the Paenibacillus polymixa CT43 strain is each introduced into 3 containers at a rate of 3 ⁇ 10 9 CFU/liter of soil. The 3 remaining tanks are not inoculated and serve as a control.
- the basil (Basilicum genovese) seeds used were of the Eleonora ENZ A Z ADEN variety.
- the seeds were planted in a germination compost composed of coconut fibres, peat and perlite (FLORENTAISE) and an inoculum comprising the strain Paraburkolderia graminis Bio Tl 19, the strain Mistuaria noduli Bio T025 (CNCM-1- 5724) or the Paenibacillus polymixa CT43 strain is introduced per tray at a rate of 1.5 ⁇ 10 9 CFU/liter of germination compost. A second inoculation is then performed with the different strains as soon as the cotyledons of the basil plants have emerged.
- a germination compost composed of coconut fibres, peat and perlite (FLORENTAISE) and an inoculum comprising the strain Paraburkolderia graminis Bio Tl 19, the strain Mistuaria noduli Bio T025 (CNCM-1- 5724) or the Paenibacillus polymixa CT43 strain is introduced per tray at a rate of 1.5 ⁇ 10 9 CFU/liter of germination compost.
- This inoculation is carried out by subirrigation and is intended to ensure homogeneous colonization of the bacteria around the roots (inoculation dose: 1.5 x 10 9 CFU/litre of soil). Of the 3 controls are not inoculated.
- the plants are then transplanted into 20 tubs containing 3.6 kg of THREL (Florentaise) compost, at the rate of 14 clumps per tub, each clump containing 2 basil plants.
- THREL Floraise
- An inoculum comprising the Paraburkolderia graminis Bio T1 19 strain, the Mistuaria noduli Bio T025 (CNCM-1-5724) strain or the Paenibacillus pofymixa CT43 strain is each introduced into 4 tanks at a rate of 3 x 10 9 CFU/ liter of potting soil and on plants that have been previously inoculated with the same strain.
- the 4 remaining tanks are not inoculated and correspond to those which have not been previously inoculated; they serve as controls.
- Two distinct water regimes are each applied to two tanks inoculated with a specific bacterial strain and to two control tanks, with:
- the basil plants are cut several times (approximately one cut every 10-12 days) before the final harvest at the end of cultivation.
- Sensation winter rapeseed seeds (SEMENCES DE FRANCE) are placed in a pot (500 ml) containing clay soil (dominance of sunflower and rapeseed).
- a spraying of 0.5 mL of solution is carried out, whether or not containing an inoculum of 10 9 CFU/ml of the strain Paraburkolderia graminis Bio Tl 19, the strain Mistuaria noduli Bio T025 (CNCM-1-5724) , or the strain Mistuaria noduli Bio T027 (CNCM-I5725).
- the culture is then carried out in a greenhouse for 8 weeks. At the end of this period, the aerial dry matter and the root dry matter are determined for each plant. The results are shown in Table IV.
- Naturala variety sunflower seeds (CAUSSADE SEMENCES) are coated or not at a rate of 10 5 CFU/seed of the strain Par aburkolderia graminis Bio T1 19 or of the strain Mistuaria noduli Bio T025 (CNCM-1-5724 ).
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- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18/698,675 US20240415128A1 (en) | 2021-10-04 | 2022-10-03 | Composition comprising a strain of mitsuaria noduli |
EP22790327.5A EP4412459A1 (fr) | 2021-10-04 | 2022-10-03 | Composition comprenant une souche de mitsuaria noduli |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FRFR2110486 | 2021-10-04 | ||
FR2110486A FR3127675A1 (fr) | 2021-10-04 | 2021-10-04 | composition comprenant une souche de Mitsuaria noduli |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2023057375A1 true WO2023057375A1 (fr) | 2023-04-13 |
Family
ID=78212376
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2022/077447 WO2023057375A1 (fr) | 2021-10-04 | 2022-10-03 | Composition comprenant une souche de mitsuaria noduli |
Country Status (4)
Country | Link |
---|---|
US (1) | US20240415128A1 (fr) |
EP (1) | EP4412459A1 (fr) |
FR (1) | FR3127675A1 (fr) |
WO (1) | WO2023057375A1 (fr) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2110486A5 (fr) | 1969-03-10 | 1972-06-02 | Eagle Picher Ind Inc | |
WO2008049230A1 (fr) * | 2006-10-24 | 2008-05-02 | University Of Manitoba | Gène ptra et ses utilisations |
WO2018186307A1 (fr) * | 2017-04-04 | 2018-10-11 | 国立大学法人岐阜大学 | Souche appartenant au genre mitsuaria et pesticide microbien utilisant ladite souche |
WO2018186316A1 (fr) * | 2017-04-04 | 2018-10-11 | 国立大学法人岐阜大学 | Pesticide microbien utilisant une souche appartenant au genre mitsuaria et une souche appartenant au genre ralstonia en combinaison |
-
2021
- 2021-10-04 FR FR2110486A patent/FR3127675A1/fr active Pending
-
2022
- 2022-10-03 EP EP22790327.5A patent/EP4412459A1/fr active Pending
- 2022-10-03 US US18/698,675 patent/US20240415128A1/en active Pending
- 2022-10-03 WO PCT/EP2022/077447 patent/WO2023057375A1/fr active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2110486A5 (fr) | 1969-03-10 | 1972-06-02 | Eagle Picher Ind Inc | |
WO2008049230A1 (fr) * | 2006-10-24 | 2008-05-02 | University Of Manitoba | Gène ptra et ses utilisations |
WO2018186307A1 (fr) * | 2017-04-04 | 2018-10-11 | 国立大学法人岐阜大学 | Souche appartenant au genre mitsuaria et pesticide microbien utilisant ladite souche |
WO2018186316A1 (fr) * | 2017-04-04 | 2018-10-11 | 国立大学法人岐阜大学 | Pesticide microbien utilisant une souche appartenant au genre mitsuaria et une souche appartenant au genre ralstonia en combinaison |
Non-Patent Citations (2)
Title |
---|
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; January 2018 (2018-01-01), FAN MIAO-CHUN ET AL: "Mitsuaria noduli sp nov., isolated from the root nodules of Robinia pseudoacacia in a lead-zinc mine", XP002806491, Database accession no. PREV201800359406 * |
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 68, no. 1, January 2018 (2018-01-01), pages 87 - 92, ISSN: 1466-5026(print), DOI: 10.1099/IJSEM.0.002459 * |
Also Published As
Publication number | Publication date |
---|---|
US20240415128A1 (en) | 2024-12-19 |
FR3127675A1 (fr) | 2023-04-07 |
EP4412459A1 (fr) | 2024-08-14 |
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