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WO2022126486A1 - Cellule nerveuse modifiée, son procédé de préparation et son application - Google Patents

Cellule nerveuse modifiée, son procédé de préparation et son application Download PDF

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Publication number
WO2022126486A1
WO2022126486A1 PCT/CN2020/137206 CN2020137206W WO2022126486A1 WO 2022126486 A1 WO2022126486 A1 WO 2022126486A1 CN 2020137206 W CN2020137206 W CN 2020137206W WO 2022126486 A1 WO2022126486 A1 WO 2022126486A1
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engineered
pluripotent stem
medium
induced pluripotent
cell
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PCT/CN2020/137206
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English (en)
Chinese (zh)
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陈爽
洪伟
陈平
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深圳先进技术研究院
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Priority to PCT/CN2020/137206 priority Critical patent/WO2022126486A1/fr
Publication of WO2022126486A1 publication Critical patent/WO2022126486A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cellule nerveuse modifiée, son procédé de préparation et son application. La cellule nerveuse modifiée est dérivée d'une cellule souche pluripotente induite modifiée ; et la cellule souche pluripotente induite modifiée est une cellule souche pluripotente induite contenant des plasmides, les plasmides comprenant pTet-O-Ngn2-puro, Tet-O-FUW-EGFP, et FUdeltaGW-rtTA. Les cellules souches pluripotentes induites génétiquement modifiées sont rapidement préparées à grande échelle au moyen d'une transduction de vecteur viral, puis sont induites par un antibiotique et soumises à une culture de criblage et de différenciation pour obtenir une cellule nerveuse modifiée, et enfin, la cellule nerveuse modifiée est induite à l'aide d'une protéine beta-amyloïde de façon à obtenir une cellule ayant un phénotype de maladie d'Alzheimer. L'ensemble du processus de préparation est simple dans son fonctionnement et court dans sa durée, et une préparation à grande échelle peut être mise en œuvre. Ainsi, un matériau expérimental reproductible et aux propriétés uniformes est présenté pour le développement et la recherche de médicaments.
PCT/CN2020/137206 2020-12-17 2020-12-17 Cellule nerveuse modifiée, son procédé de préparation et son application WO2022126486A1 (fr)

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PCT/CN2020/137206 WO2022126486A1 (fr) 2020-12-17 2020-12-17 Cellule nerveuse modifiée, son procédé de préparation et son application

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PCT/CN2020/137206 WO2022126486A1 (fr) 2020-12-17 2020-12-17 Cellule nerveuse modifiée, son procédé de préparation et son application

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105247070A (zh) * 2013-03-15 2016-01-13 怀特黑德生物医学研究院 用于神经变性疾病的细胞性发现平台

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105247070A (zh) * 2013-03-15 2016-01-13 怀特黑德生物医学研究院 用于神经变性疾病的细胞性发现平台

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
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