WO2021172269A1 - Yellow-dulling remediation agent, cosmetic composition, and yellow-dulling remediation method - Google Patents

Abstract

The present invention provides a yellow-dulling remediation agent containing β-1,3-1,6-glucan, a cosmetic composition containing same, and a yellow-dulling remediation method.

Description

Yellowing dullness improving agent, cosmetic composition and yellowing dullness improving method

The present invention relates to a yellowing improving agent, a cosmetic composition, and a yellowing improving method.

There are various types of dullness of the skin, and dullness due to decreased transparency, dullness due to poor blood circulation, and dullness due to yellowing of the skin are known (Patent Document 1). Dullness due to yellowing of the skin is known as yellowing. Yellow dullness is caused by the accumulation of carbonylated proteins in the skin due to aging, stress, and exposure to ultraviolet rays, and the accumulation of AGEs (advanced glycation end products) in the skin due to aging and excessive sugar intake. , Caused.

In order to suppress yellowing, a component that suppresses protein carbonylation (Patent Document 2) and a component that suppresses the production of AGEs (Patent Document 3) have been searched for as active ingredients of cosmetic compositions. However, it is not sufficient to suppress yellowing, and yellowing is a cause of cosmetological problems.

β-glucan is beneficial because it shows improvement in skin quality in combination with resident starch and fructan in a specific amount (Patent Document 4), and also shows a moisturizing effect on the skin in combination with heat-treated cells of lactic acid bacteria (Patent Document 5). It is known to be an ingredient.

JP-A-2017-178899 Japanese Unexamined Patent Publication No. 2012-031106 Japanese Unexamined Patent Publication No. 2011-102270 Japanese Unexamined Patent Publication No. 2018-52847 Japanese Unexamined Patent Publication No. 2004-269408

As described above, the conventional ingredients for improving yellowing are not satisfactory, and the development of a new yellowing improving agent has been required.
Therefore, an object of the present invention is to provide a yellowing improving agent, a cosmetic composition, and a yellowing improving method capable of improving the yellowing of the skin.

As a result of various studies to solve the above problems, the present inventors have found that β-1,3-1,6-glucan can remarkably improve the yellowing of the skin, and completed the present invention.
That is, the present invention is a yellowing improving agent containing β-1,3-1,6-glucan.

Further, the present invention is a cosmetic composition containing 0.001 to 1% by mass of the above-mentioned yellowing improving agent as β-1,3-1,6-glucan.
Further, the present invention is a method for improving yellowing, which comprises applying the above cosmetic composition to the skin.

Further, the present invention is the use of a yellowing dullness improving agent containing β-1,3-1,6-glucan or a cosmetic composition containing the yellowing dullness improving agent for improving yellowing.
The present invention also relates to the use of β-1,3-1,6-glucan for preparing a yellowing improving agent or for preparing a cosmetic composition containing the yellowing improving agent. be.

The yellowing improving agent of the present invention can also be referred to as an "external composition for improving yellowing of the skin".

By using the yellowing improving agent of the present invention and the cosmetic composition containing the yellowing improving agent, the yellowing of the skin can be improved.

The yellowing improving agent of the present invention contains β-1,3-1,6-glucan.

β-1,3-1,6-glucan refers to β-glucose in which glucose is linked by β-1,3-glycosidic bond and β-1,6-glycosidic bond, and (1) the main chain is β-. 1,3-Glycosidic bond with branch of β-1,6-glycosidic bond, (2) Main chain consisting of β-1,3-glycosidic bond and β-1,6-glycosidic bond Etc. are known. As the β-1,3-1,6-glucan used in the present invention, those having a bond such as β-1,4-glycosidic bond may be used, but β-1,3-glycosidic bond is used. The content of glucosidic bonds other than the bonds and β-1,6-glycosidic bonds is preferably 10 mol% or less with respect to the total of β-1,3-glycosidic bonds and β-1,6-glycosidic bonds. ..

As the β-1,3-1,6-glucan, any β-1,3-1,6-glucan can be used as the yellowing improving agent of the present invention, but the yellowing of the skin (1) The main chain is a β-1,3-glycosidic bond, and it is preferable that the β-1,6-glycosidic bond is branched. Among them, the following general Β-1,3-1,6-glucan represented by the formula (1) is preferable:

In the above general formula (1), R ¹ represents a hydrogen atom or a group represented by the following formula (A), and ^{one or more of a plurality of R 1} is the following formula (A). a represents a number having a mass average molecular weight of β-1,3-1,6-glucan represented by the general formula (1) of 3,000 to 5,000,000. Both ends of β-1,3-1,6-glucan represented by the general formula (1) are hydroxyl groups, and * represents a bond. Further, among the plurality of R ¹ , preferably 16 or more are hydrogen atoms, and more preferably 20 or more are hydrogen atoms. That is, from the viewpoint of exerting the effect of the present invention, in the above general formula (1), R ¹ represents a hydrogen atom or a group represented by the following formula (A), and ^{one or more of a plurality of R 1} is the following formula. a (a), it is preferable more than 20 among a plurality of R ¹ is a hydrogen atom.
Further, among the plurality of R ¹ , the hydrogen atom is preferably 31,000 or less, more preferably 28,000 or less, and further preferably 23,000 or less.

(In formula (A), * represents a bond.)

When the mass average molecular weight of β-1,3-1,6-glucan represented by the general formula (1) is less than 3,000 and greater than 5,000,000, the yellowing of the skin is improved. The effect may be insufficient. The mass average molecular weight of β-1,3-1,6-glucan represented by the general formula (1) is preferably 5,000 to 3,000,000, more preferably 7,000 to 1,000,000. Most preferably 10,000 to 500,000. In the present invention, the mass average molecular weight of β-1,3-1,6-glucan is the pullulan equivalent when gel permeation chromatography (also referred to as GPC) analysis is performed using water as a solvent. Mass average molecular weight. The "mass average molecular weight" may also be referred to as a "weight average molecular weight".

The method for measuring the mass average molecular weight is not particularly limited, but as an example, a calibration curve (holding time on the x-axis) using a differential refractometer using several types of pullruns having a known molecular weight Create a molecular weight on the y-axis). Next, it is common to measure a sample (concentration is arbitrary) and calculate the relative molecular weight (molecular weight in terms of pullulan) from the retention time using the calibration curve.
However, pullulan and β-1,3-1,6-glucan have different molecular sizes even if they have the same molecular weight (same number of glucose units) due to the difference in structure, so only relative values can be obtained by gel permeation chromatography, which is a molecular sieve. However, it is possible to calculate the intrinsic molecular weight close to the absolute value by correcting it. Examples of the correction include a method of measuring the concentration of a sample and measuring and calculating three points of differential pressure viscosity, differential refractometer, and light scattering. Specifically, first, a pullulan standard solution having a known concentration, viscosity, and refractive index is used to determine the device constant k.

Next, the drug substance whose concentration of β-1,3-1,6-glucan was measured by the phenol-sulfuric acid method is precisely measured, and an aqueous solution having a known concentration is prepared as a sample. Then, the molecular weight is calculated from the values measured by the differential pressure viscosity detector, the differential refractometer detector, and the light scattering detector.

In formula (1), (Unit Unit / R ¹ wherein R ¹ is formula (A) is a hydrogen atom) ratio of units wherein R ¹ is formula (A) with respect to unit R ¹ is a hydrogen atom Is preferably 0.2 to 1, more preferably 0.5 to 0.95, and most preferably 0.6 to 0.9, from the viewpoint of suppressing yellowing of the skin. preferable. Incidentally, in the general formula (1), the unit unit and R ¹ R ¹ is a hydrogen atom is an expression (A), may be bonded in a random form, it is attached to the block-shaped good.

^{The ratio of the unit in which R 1} is the formula (A) to the unit in which R ¹ is a hydrogen atom is obtained by the following method. First, after methylating the free hydroxyl group of β-1,3-1,6-glucan, β-1,3-1,6-glucan is acid-hydrolyzed to a monosaccharide to acetylate the hydroxyl group generated by the decomposition. .. In the obtained glucose derivative, the hydroxyl group that contributed to the bond is acetylated, and the hydroxyl group that does not contribute to the bond is methylated. Various glucose derivatives are quantified by gas chromatography (GC) or the like. do. This makes it possible to calculate the ratio of the glucose derivative having a β-1,3 bond to the glucose derivative having a β-1,3 bond and a β-1,6 bond. Further, β-1,3-1,6-glucan is analyzed in detail using 13C-NMR or the like to calculate the ratio of one branch to two glucose residues and one branch to three glucose residues.
From these calculated values, the ratio of the above units can be obtained.

Β-1,3-1,6-glucan represented by the general formula (1) is a known method, for example, yeast, lactic acid bacterium, natto bacterium, acetic acid bacterium, aspergillus, algae such as chlorella and spirulina, filamentous fungus and the like. It can be obtained by extracting from the cell wall and product of yeast. Among them, β-1,3-1,6-glucan, which has a high moisturizing effect on the skin, can be extracted from black yeast (Aureobasidium pullulans). The method for extracting β-1,3-1,6-glucan is not particularly limited, and a known method may be used.

As an example of the black yeast, the deposit number FERM BP-8391 can be used in the present invention.
The details of the deposit number FERM BP-8391 are explained in International Publication No. 2004/001053 (WO2004 / 001053), and the deposit number is deposited at the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology. .. The details that identify the deposit are described below.

(1) Depositary Institution Name: National Institute of Advanced Industrial Science and Technology (Currently: National Institute of Advanced Industrial Science and Technology) Patent Biological Deposit Center (Current Depositary Institution Name: Incorporated Administrative Agency Product Evaluation Technology Infrastructure Organization Patent Biological Deposit Center) )
(2) Contact: 1-1-1, Higashi, Tsukuba-shi, Ibaraki, Japan 305-8566 Central 6th
(Current contact information :: 〒292-0818 Room 2-5-8 Kazusakamatari, Kisarazu City, Chiba Prefecture)
(3) Contract number: FERM BP-8391
(4) Display for identification: Aureobasidium pullulans ADK-34
(5) Original deposit date: July 11, 2002 (6) Transfer date to deposit based on the Budapest Convention: June 2, 2003

Further, when the extracted β-1,3-1,6-glucan is concentrated, it is not particularly limited as long as it is a method for removing water or powdering. For example, spray drying, freeze drying, vacuum drying, heat drying, precipitation recovery with a solvent and the like can be mentioned, and these can be appropriately combined.

The yellowing improving agent of the present invention can be used in products such as pharmaceuticals, quasi-drugs, and cosmetics. It is also possible to prepare a product by blending an additive or the like necessary for the yellowing improving agent of the present invention, and adding an appropriate amount of the yellowing improving agent of the present invention to the raw material of the product (β-1,3-1). , 6-Glucan premix composition).

Since the yellowing improving agent of the present invention can be easily prepared in the cosmetic composition described later, for example, β-1,3-1,6 is applied to the total amount of the yellowing improving agent. -Glucan as a solid content is preferably contained in an amount of 0.001% by mass or more, more preferably 0.01% by mass or more, further preferably 0.1% by mass or more, and 0.5% by mass. It is even more preferably contained in an amount of 0.7% by mass or more, and most preferably 0.7% by mass or more. The upper limit of β-1,3-1,6-glucan is not particularly limited as long as the effect of the present invention can be obtained, but from the viewpoint of high fluidity and good handleability, the yellowing improving agent The above β-1,3-1,6-glucan as a solid content is preferably contained in an amount of 20% by mass or less, more preferably 10% by mass or less, and 5.0% by mass or less based on the total amount. It is more preferably contained in an amount of 3.0% by mass or less, and more preferably 2.0% by mass or less.

In the yellowing improving agent of the present invention, when the β-1,3-1,6-glucan is in the form of an aqueous solution, the concentration of the β-1,3-1,6-glucan is 2% by mass or more. It may not be completely dissolved and precipitation may easily occur. In this case, by containing 50 to 90% by mass of a polyhydric alcohol having 2 to 6 carbon atoms with respect to the yellowing improving agent, it becomes easy to use as an external composition.

Examples of the polyvalent alcohol having 2 to 6 carbon atoms include ethylene glycol, diethylene glycol, triethylene glycol, tetraethylene glycol, propylene glycol, dipropylene glycol, 1,3-propanediol, 1,2-butanediol, and 1, , 3-Butanediol, 1,4-Butanediol, 2,3-Butanediol, 2-Methyl-1,2-Propanediol, 2-Methyl-1,3-Propanediol, 1,2-Pentanediol, 1, , 3-Pentanediol, 1,4-Pentanediol, 1,5-Pentanediol, 2,3-Pentanediol, 2,4-Pentanediol, 2-Methyl-1,2-Butanediol, 2-Methyl-2 , 3-butanediol, 2-methyl-1,4-butanediol, 3-methyl-1,2-butanediol, 3-methyl-1,3-butanediol, 2-ethyl-1,3-propanediol, 2,2-dimethyl-1,3-propanediol, 1,5-hexanediol, 1,6-hexanediol, 2,5-hexanediol, 3,3-hexanediol, 2-methyl-1,3-pentane Didiol, 2-methyl-1,5-pentanediol, 2-methyl-2,4-pentanediol, 3-methyl-1,5-pentanediol, 3-methyl-2,3-pentanediol, 3-methyl- Divalent alcohols such as 2,4-pentanediol, 2-ethyl-2-methyl-1,3-propanediol, 2,2-dimethyl-2,4-butanediol or their condensates;
Fat cyclic dihydric alcohols such as 1,2-cyclopentanediol, 1,3-cyclopentanediol, 1,2-cyclohexanediol, 1,3-cyclohexanediol, and 1,4-cyclohexanediol;

Glycerin, 1,2,3-butanetriol, 1,2,4-butanetriol, 2-methyl-1,2,3-propanetriol, 1,2,3-pentanetriol, 1,2,4-pentanetriol , 1,3,5-pentantriol, 2,3,4-pentantriol, 2-methyl-2,3,4-butantriol, 2,3,4-hexanetriol, 2-ethyl-1,2,3 -Trivalent alcohols such as butane triol;
Erythritol, 1,2,3,4-pentatetrol, 2,3,4,5-hexatetrol, 1,2,4,5-pentantetrol, 1,3,4,5-hexanetetrol, Tetrahydric alcohols such as diglycerin and sorbitan;
Pentahydric alcohols such as adonitol, arabitol, xylitol and triglycerin; hexahydric alcohols such as sorbitol, mannitol, iditol, inositol, darsitol, tarose and allose;
Glycerin such as 1-methylglyceryl ether, 2-methylglyceryl ether, 1-ethyl glyceryl ether, 2-ethylglyceryl ether, 1-propyl glyceryl ether, 2-propyl glyceryl ether, 1-isopropyl glyceryl ether, 2-isopropyl glyceryl ether Examples include monoethers.

Among these polyhydric alcohols, dihydric alcohol or trihydric alcohol is preferable because β-1,3-1,6-glucan has excellent dispersion stability, and propylene glycol, dipropylene glycol, and 1,3-butane are preferable. Diol and glycerin are more preferable, and 1,3-butanediol is further preferable.

When the polyhydric alcohol is contained in an amount of 50 to 90% by mass as described above, the yellowing improving agent of the present invention has excellent dispersion stability of β-1,3-1,6-glucan, and therefore water. The blending amount of is preferably 5 to 27% by mass.

The yellowing improving agent of the present invention is preferably blended in a cosmetic composition.
The yellowing improving agent of the present invention contains various compounding ingredients that can be usually used in cosmetics depending on the purpose of use, such as various surfactants, various enzymes, drugs, antibacterial agents (preservatives), isotonic agents, and buffers. Agents, stabilizers, chelating agents, solubilizing agents, pH adjusters, fragrances and the like can be blended as appropriate in a range of normal usage amounts.

In the yellowing improving agent of the present invention, an antibacterial agent (preservative) that can be contained in the cosmetic composition described later can be used, but from the viewpoint of exerting the effect of the present invention, phenoxyethanol, ethylhexyl glycerin and the like can be used. Ether derivative type is preferable. The antibacterial agent can be used in the same blending amount as in the cosmetic composition.

Next, the cosmetic composition of the present invention will be described.
The cosmetic composition of the present invention may be in any form such as an aqueous solution containing substantially no oil, a gel-like cosmetic, an emulsified cosmetic, or a solid content dispersed therein. In the case of an emulsified cosmetic, it can be provided in the form of either an oil-in-water type or a water-in-oil emulsion, but is like the oil-in-water type or the water-in-oil type, that is, continuous. It is preferable that the phase is an aqueous cosmetic which is an aqueous phase.

The cosmetic composition of the present invention contains the above-mentioned yellowing improving agent, and is contained in the cosmetic composition from the viewpoint of satisfactorily exerting the effect of improving the yellowing of the skin and from the viewpoint of manufacturing cost. As β-1,3-1,6-glucan (solid content), preferably 0.0001 to 1% by mass, more preferably 0.0005 to 1% by mass, still more preferably, based on the total amount of the cosmetic composition. Is 0.001 to 1% by mass, even more preferably 0.005 to 0.5% by mass, still more preferably 0.005 to 0.2% by mass, and even more preferably 0.005 to 0.1% by mass. Most preferably, it is contained so as to be 0.005 to 0.01% by mass.

From the viewpoint of sufficiently exerting the effects of the present invention, it is preferable that β-1,3-1,6-glucan is contained in an amount of 0.0001% by mass or more in terms of solid content with respect to the total amount of the cosmetic composition. From the viewpoint of ease of preparation of the cosmetic composition and prevention of change in usability, β-1,3-1,6-glucan is contained in an amount of 1% by mass or less in terms of solid content with respect to the total amount of the cosmetic composition. Is preferable.

The cosmetic composition of the present invention can contain any ingredient that can be usually used in cosmetics, in addition to the above-mentioned yellowing improving agent. For example, surfactants, chelating agents, low molecular weight polyhydric alcohols, plant-derived extracts, animal-derived extracts, microbial-derived extracts, microbial cultures, zinc compounds, thickeners, oils, pigments, pigments, antibacterial agents (preservatives). ), Moisturizers, pH adjusters, antioxidants, UV absorbers, moisturizing ingredients, enzymes, fragrances and the like. Cultures with microorganisms include whole cultures containing microorganisms and cultures, supernatants, microorganisms, or whole cultures, lyophilized supernatants or microorganisms, concentrates or extracts.

Examples of the surfactant include anionic surfactants, nonionic surfactants, cationic surfactants and amphoteric surfactants. Examples of the anionic surfactant include higher fatty acid salt, higher alcohol sulfate ester salt, sulfated olefin salt, higher alkyl sulfonate, α-olefin sulfonate, sulfated fatty acid salt, sulfonated fatty acid salt, and phosphoric acid ester. Salts, fatty acid ester sulfates, glyceride sulfates, fatty acid ester sulfonates, α-sulfo fatty acid methyl ester salts, polyoxyalkylene alkyl ether sulfates, polyoxyalkylene alkylphenyl ether sulfates, polyoxy Alkylene alkyl ether carboxylate, acylated peptide, fatty acid alkanolamide or sulfate ester salt of its alkylene oxide adduct, sulfosuccinate, alkylbenzene sulfonate, alkylnaphthalene sulfonate, alkylbenzoimidazole sulfonate, polyoxyalkylene Sulfosuccinate, salt of N-acyl-N-methyltaurine, N-acylglutamic acid or its salt, acyloxyethane sulfonate, alkoxyethane sulfonate, N-acyl-β-alanine or its salt, N-acyl- Examples thereof include N-carboxyethyl taurine or a salt thereof, N-acyl-N-carboxymethylglycine or a salt thereof, an acyl lactate, an N-acylsulfosin salt, and an alkyl or alkenylaminocarboxymethyl sulfate.

Examples of the nonionic surfactant include polyoxyalkylene alkyl ether, polyoxyalkylene alkenyl ether, and polyoxyethylene polyoxypropylene alkyl ether (the addition form of ethylene oxide and propylene oxide may be random or block. ), Polyethylene glycol propylene oxide adduct, Polyethylene glycol ethylene oxide adduct, Glycerin fatty acid ester or ethylene oxide adduct thereof, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, alkylpolyglucoside, fatty acid monoethanolamide or ethylene oxide addition thereof. Product, fatty acid-N-methylmonoethanolamide or ethylene oxide adduct thereof, fatty acid diethanolamide or ethylene oxide adduct thereof, sucrose fatty acid ester, alkyl (poly) glycerin ether, polyglycerin fatty acid ester, polyethylene glycol fatty acid ester, fatty acid Examples thereof include methyl ester ethoxylate and N-long chain alkyldimethylamine oxide.

Examples of the cationic surfactant include an alkyl (alkenyl) trimethylammonium salt, a dialkyl (alkenyl) dimethylammonium salt, an alkyl (alkenyl) quaternary ammonium salt, and a mono or dialkyl (alkenyl) containing an ether group or an ester group or an amide group. ) Quaternary ammonium salt, alkyl (alkenyl) pyridinium salt, alkyl (alkenyl) dimethylbenzylammonium salt, alkyl (alkenyl) isoquinolinium salt, dialkyl (alkenyl) morpholinium salt, polyoxyethylene alkyl (alkenyl) amine, alkyl (alkenyl) amine Examples thereof include salts, polyamine fatty acid derivatives, amyl alcohol fatty acid derivatives, benzalconium chloride, and benzethonium chloride.

Examples of amphoteric surfactants include carboxybetaine, sulfobetaine, phosphobetaine, amide amino acids, and imidazolinium betaine-based surfactants.

The above-mentioned surfactant may be blended with one or more of the above-mentioned one or more. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.01 to 80% by mass with respect to the total amount of the cosmetic composition. More preferably, it is 05 to 60% by mass.

Examples of the chelating agent include an aminopolycarboxylic acid chelating agent, an aromatic or aliphatic carboxylic acid chelating agent, an amino acid chelating agent, an ether polycarboxylic acid chelating agent, a phosphonic acid chelating agent, and a phosphoric acid chelating agent. Examples thereof include hydroxycarboxylic acid chelating agents, high molecular weight electrolyte (including oligomeric electrolyte) chelating agents, dimethylglioxime, ascorbic acid, thioglycolic acid, phytic acid, glyoxylic acid, and glyoxal acid. Each of these chelating agents may be in the form of a free acid or in the form of a salt such as a sodium salt, a potassium salt or an ammonium salt. In addition, they may be in the form of their hydrolyzable ester derivatives.

Examples of the aminopolycarboxylic acid-based chelating agent include ethylenediaminetetraacetic acid, ethylenediaminediacetic acid, cyclohexanediaminetetraacetic acid, nitrilotriacetic acid, iminodiacetic acid, N- (2-hydroxyethyl) iminodiacetic acid, diethylenetriaminepentaacetic acid, and N- (2). -Hydroxyethyl) ethylenediaminetriacetic acid, glycol etherdiaminetetraacetic acid, glutamate diacetic acid, aspartate diacetic acid and salts thereof.

Examples of aromatic or aliphatic carboxylic acid-based chelating agents include oxalic acid, malonic acid, succinic acid, glutaric acid, adipic acid, pimelic acid, sebacic acid, azelaic acid, itaconic acid, aconitic acid, pyruvate, and salicylic acid. Examples thereof include acetylsalicylic acid, hydroxybenzoic acid, aminobenzoic acid (including anthranylic acid), phthalic acid, fumaric acid, trimellitic acid, gallic acid, hexahydrophthalic acid and salts thereof, methyl esters and ethyl esters.

Examples of amino acid-based chelating agents include glycine, serine, alanine, lysine, cystine, cysteine, ethionine, tyrosine, methionine and salts and derivatives thereof.
Examples of the phosphonic acid-based chelating agent include iminodimethylphosphonic acid, alkyldiphosphonic acid, 1-hydroxyethane-1,1-diphosphonic acid and salts thereof.
Examples of the phosphoric acid-based chelating agent include orthophosphoric acid, pyrophosphoric acid, triphosphoric acid and polyphosphoric acid.

Examples of the hydroxycarboxylic acid-based chelating agent include malic acid, citric acid, glycolic acid, gluconic acid, heptonic acid, tartaric acid, lactic acid and salts thereof.
Examples of the polymer electrolyte (including oligomeric electrolyte) chelating agent include an acrylic acid polymer, a maleic anhydride polymer, an α-hydroxyacrylic acid polymer, an itaconic acid polymer, and two constituent monomers of these polymers. Examples thereof include a copolymer composed of the above and an epoxy succinic acid polymer.

Among these chelating agents, ethylenediaminetetraacetic acid, ethylenediaminediacetic acid, nitrilotriacetic acid, iminodiacetic acid, N- (2-hydroxyethyl) iminodiacetic acid, aspartate diacetic acid, and succinic acid are highly safe and have a large chelating effect. Acids, salicylic acid, oxalic acid, lactic acid, fumaric acid, citric acid, tartrate, 1-hydroxyethane-1,1-diphosphonic acid and salts thereof are preferred.

The chelating agent may be a mixture of one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.01 to 30% by mass with respect to the total amount of the cosmetic composition. More preferably, it is 05 to 20% by mass.

The low molecular weight polyvalent alcohol is preferably a polyvalent alcohol having a molecular weight of 50 to 1000 and a molecular weight of 50 to 500 from the viewpoint of exerting the effect of the present invention. For example, ethylene glycol, diethylene glycol, triethylene glycol, tetraethylene glycol, etc. Propylene glycol, dipropylene glycol, tripropylene glycol, 1,3-propanediol, 1,2-butanediol, 1,3-butanediol, 1,4-butanediol, 2,3-butanediol, 2-methyl- 1,2-Propanediol, 2-methyl-1,3-propanediol, 1,2-pentanediol, 1,3-pentanediol, 1,4-pentanediol, 1,5-pentanediol, 2,3- Pentandiol, 2,4-pentanediol, 2-methyl-1,2-butanediol, 2-methyl-2,3-butanediol, 2-methyl-1,4-butanediol, 3-methyl-1,2 -Butandiol, 3-methyl-1,3 butanediol, 2-ethyl-1,3-propanediol, 2,2-dimethyl-1,3-propanediol, 1,2-hexanediol, 1,5-hexane Diole, 1,6-hexanediol, 2,5-hexanediol, 3,3-hexanediol, 2-methyl-1,3-pentanediol, 2-methyl-1,5-pentanediol, 2-methyl-2 , 4-Pentanediol, 3-Methyl-1,5-Pentanediol, 3-Methyl-2,3-Pentanediol, 3-Methyl-2,4-Pentanediol, 4-Methyl-1,2-Pentanediol, 2-Ethyl-2-methyl-1,3-propanediol, 2,2-dimethyl-1,3-butanediol, 2,3-dimethyl-1,2-butanediol, 1,2-heptandiol, 1, 7-Heptanediol, 3,4-Heptanediol, 2-propyl-2-methyl-1,3-propanediol, 2-isopropyl-2-methyl-1,3-propanediol, 1,2-octanediol, 1 , 8-octanediol, 3,6-octanediol, 2-ethyl-1,3-hexanediol, 2-sec-butyl-2-butyl-1,3-propanediol, 2,2-dimethyl-1,3 -Hexanediol, 2,5-dimethyl-2,5-hexanediol, 2,2,4-trimethyl-1,3-pentanediol, 1,2-nonanediol, 1,3-nonanediol, 1, 9-Nonandiol, 3,6-octanediol, 2-ethyl-2- (2-methyl) propyl-1,3-propanediol, 2,2,4-trimethyl-1,6-hexanediol, 2,4 , 4-trimethyl-1,6-hexanediol, 2-butyl-2-ethyl-1,3-propanediol, 2,4-diethyl-1,5-pentanediol, 2-methyl-1,8-octanediol , 1,2-decanediol, 1,10-decanediol, 5,6-decanediol, 3,6-dimethyl-3,6-octanediol, 3,7-dimethyl-1,6-octanediol, 3, 7-Dimethyl-1,7-octanediol, 1,2-undecanediol, 1,4-undecanediol, 1,11-undecanediol, 6-ethyl-3-methyl-1,6-octanediol, 1,2 -Dodecanediol, 1,10-dodecanediol, 1,12-dodecanediol, 2,4-diethyl-1,5-octanediol, 2,8,8-trimethyl-2,7-nonanediol, 1,2- Tetradecanediol, 1,14-tetradecanediol, 7,8-tetradecanediol, 1,2-pentadecanediol, 1,2-hexadecandiol, 1,16-hexadecandiol, 1,17-heptadecanediol, 1,2- Saturated diols such as octadecanediol, 1,12-octadecanediol, 1,2-eicosanediol, 1,2-docosanediol, 1,2-tetracosanediol or condensates thereof;

2-Buten-1,4-diol, 3-butene-1,2-diol, 2-methylene-1,3-propanediol, 2-butyne-1,4-diol, 5-hexene-1,2-diol , 3-Methyl-2-pentene-1,5-diol, 3-methylenepentyne-1,5-diol, 1,5-hexadien-3,4-diol, 7-octene-1,2-diol, 2, 5-Dimethyl-3-hexene-2,5-diol, 9-decene-1,2-diol, 2,6-dimethyl-7-octene-2,6-diol, 3,7-dimethyl-7-octene- 1,6-diol, 13-tetradecene-1,2-diol, 12-hydroxy-9-octadesenol, 2-pentyne-1,4-diol, 3-hexin-2,5-diol, 4-methyl-2- Pentin-1,4-diol, 3-heptin-2,5-diol, 4-methyl-2-pentyne-1,4-diol, 3,4-dimethyl-1-pentyne-3,4-diol, 2, 5-Dimethyl-3-hexin-2,5-diol, 5-decine-4,7-diol, 2,6-dimethyl-7-octyne-2,6-diol, 3,6-dimethyl-4-octyne- 3,6-diol, 2,3,6,7-tetramethyl-4-octyne-3,6-diol, 2,4,7,9-tetramethyl-5-decine-4,7-diol, 2, 5,8,11-Tetramethyl-6-dodecine-5,8-diol, 1,1,4,4-tetraisopropyl-4-octyne-3,6-diol, 5,10-diethyl-7-tetradecine- Unsaturated diols such as 6,9-diol;

1,2-Cyclopentanediol, 1,3-cyclopentanediol, 1,2-cyclohexanediol, 1,3-cyclohexanediol, 1,4-cyclohexanediol, 1,2-cycloheptanediol, 2,3-norbornane Glycol, 2,5-norbornandiol, 2,7-norbornandiol, 1,2-cyclooctanediol, 1,4-cyclooctanediol, 1,2-cyclodecanediol, 5-cyclooctene-1,2-diol , 1,5-decalindiol, limonene glycol, 1,2-terpenediol, 4,4'-bicyclohexanediol, 1,2-cyclododecanediol and other alicyclic diols;

Glycerin, 1,2,3-butanetriol, 1,2,4-butanetriol, 2-methyl-1,2,3-propanetriol, 1,2,3-pentanetriol, 1,2,4-pentanetriol , 1,3,5-pentantriol, 2,3,4-pentantriol, 2-methyl-2,3,4-butanetriol, trimethylolethane, 2,3,4-hexanetriol, 2-ethyl-1 , 2,3-Butantriol, Trimethylol Propane, 4-propyl-3,4,5-Heptanetriol, 2,4-Dimethyl-2,3,4-Pentantriol, Triethanolamine, Triisopropanolamine, etc. 3 Triol;

Ellisritol, pentaerythritol, 1,2,3,4-pentatetrol, 2,3,4,5-hexatetrol, 1,2,4,5-pentantetrol, 1,3,4,5-hexane Tetrol, diglycerin, sorbitan, N, N, N', N'-tetrakis (2-hydroxypropyl) ethylenediamine, N, N, N', N'-tetrakis (hydroxyethyl) ethylenediamine and other tetrahydric alcohols;

Pentahydric alcohols such as adonitol, arabitol, xylitol and triglycerin; hexahydric alcohols such as dipentaerythritol, sorbitol, mannitol, iditol, inositol, darsitol, tarose and allose;

1-Methyl glyceryl ether, 2-Methyl glyceryl ether, 1-ethyl glyceryl ether, 2-ethyl glyceryl ether, 1-propyl glyceryl ether, 2-propyl glyceryl ether, 1-isopropyl glyceryl ether, 2-isopropyl glyceryl ether, 1- Butyl glyceryl ether, 2-butyl glyceryl ether, 1-isobutyl glyceryl ether, 2-isobutyl glyceryl ether, 1-pentyl glyceryl ether, 2-pentyl glyceryl ether, 1-hexyl glyceryl ether, 2-hexyl glyceryl ether, 1-heptyl glyceryl Ether, 2-heptyl glyceryl ether, 1-octyl glyceryl ether, 2-octyl glyceryl ether, 1- (2-ethylhexyl) glyceryl ether, 2- (2-ethylhexyl) glyceryl ether, 1-nonyl glyceryl ether, 2-nonyl glyceryl Ether, 1-decyl glyceryl ether, 2-decyl glyceryl ether, 1-undecyl glyceryl ether, 2-undecyl glyceryl ether, 1-dodecyl glyceryl ether, 2-dodecyl glyceryl ether, 1-tridecyl glyceryl ether, 2-tri Decyl glyceryl ether, 1-tetradecyl glyceryl ether, 2-tetradecyl glyceryl ether, 1-hexadecyl glyceryl ether, 2-hexadecyl glyceryl ether, 1-octadecyl glyceryl ether, 2-octadecyl glyceryl ether, 1-branched octadecyl glyceryl Ether, 2-branched octadecyl glyceryl ether, 1-eicosyl glyceryl ether, 2-eicosyl glyceryl ether, 1-allyl glyceryl ether, 2-allyl glyceryl ether, 1-undecenyl glyceryl ether, 2-undecenyl glyceryl Glycerin monoethers such as ether, 1-oleyl glyceryl ether, 2-oleyl glyceryl ether, 1-phenyl glyceryl ether, 2-phenyl glyceryl ether;

N-substituted diethanolamines such as N-methyldiethanolamine, N-ethyldiethanolamine, N-propyldiethanolamine, N-isopropyldiethanolamine, N-butyldiethanolamine, N-cyclohexyldiethanolamine, N- (2-ethylhexyl) diethanolamine;
N-methyldiisopropanolamine, N-ethyldiisopropanolamine, N-propyldiisopropanolamine, N-isopropyldiisopropanolamine, N-butyldiisopropanolamine, N-cyclohexyldiisopropanolamine, N- (2-ethylhexyl) di N-substituted diisopropanolamines such as isopropanolamine;

3-Dimethylamino-1,2-propanediol, 3-diethylamino-1,2-propanediol, 3-dipropylamino-1,2-propanediol, 3-diisopropylamino-1,2-propanediol, 3- Examples thereof include N, N-di-substituted-3-amino-1,2-propanediols such as dibutylamino-1,2-propanediol.

Among these low molecular weight polyhydric alcohols, propylene glycol, dipropylene glycol, tripropylene glycol, 1,2-butanediol, 1,3-butanediol, and 2,3-butane from the viewpoint of exerting the effects of the present invention. Glycol, 1,2-pentanediol, 3-methyl-1,3 butanediol, 1,2-hexanediol, 1,2-heptanediol, 1,2-octanediol, 2-ethyl-1,3-hexanediol , 1,2-Nonandiol, 2-butyl-2-ethyl-1,3-propanediol, 1,2-decanediol, 1,10-decanediol, glycerin, triethanolamine, triisopropanolamine, erythritol, di Glycerin, sorbitane, N, N, N', N'-tetrakis (2-hydroxypropyl) ethylenediamine, N, N, N', N'-tetrakis (hydroxyethyl) ethylenediamine, sorbitol, 1-methylglyceryl ether, 1- Ethyl glyceryl ether, 1-propyl glyceryl ether, 1-butyl glyceryl ether, 1-octyl glyceryl ether, 1- (2-ethylhexyl) glyceryl ether, 1-decyl glyceryl ether, 1-allyl glyceryl ether are preferable, and propylene glycol and di Propylene glycol, tripropylene glycol, 1,3-butanediol, 3-methyl-1,3butanediol, 1,2-octanediol, glycerin, triethanolamine, diglycerin, sorbitan, N, N, N', N '-Tetrax (2-hydroxypropyl) ethylenediamine, sorbitol, 1-allyl glyceryl ether are more preferred, propylene glycol, dipropylene glycol, 1,3-butanediol, 3-methyl-1,3-butanediol, glycerin, sorbitol. , 1-allylglyceryl ether is more preferred.

The small molecule polyhydric alcohol may contain one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.01 to 90% by mass with respect to the total amount of the cosmetic composition. More preferably, it is 05 to 70% by mass. However, when the cosmetic composition is an aqueous cosmetic such as a lotion or an oil-in-water emulsified cosmetic, the blending amount of the low molecule polyhydric alcohol is 0.1 to 50 with respect to the total amount of the cosmetic composition. It is preferably by mass%, more preferably 1 to 20% by mass. Some small molecule polyhydric alcohols have a moisturizing effect.

The plant-derived extract is the whole plant, algae, or fungus, or a specific part such as flowers, leaves, stems, fruits, bark, roots, seeds, and resin, as it is, or by squeezing, drying, crushing, or fermenting. It is obtained by extracting with a solvent at room temperature or heating, and is soluble in water, ethanol, propylene glycol or fats and oils. Alternatively, the extract may be diluted, concentrated, or dried. Further, the essential oil may be obtained by using a steam distillation method, an extraction method, a chromatography method or the like.

As the extraction solvent for the plant-derived extract, from the viewpoint of exerting the effect of the present invention, those usually used for extracting natural components, for example, water; alcohols such as methanol, ethanol, propanol and butanol; ethylene glycol and propylene. Polyhydric alcohols such as glycol, butylene glycol, glycerin; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; ethers such as tetrahydrofuran, diethyl ether and polyethylene glycol; halogenated hydrocarbons such as dichloroethane and chloroform Hydrogens; aliphatic hydrocarbons such as petroleum ether, n-hexane and cyclohexane; aromatic hydrocarbons such as toluene; pyridines; sodium chloride solution and the like, especially water, ethanol, propylene glycol and butylene glycol. preferable.

Plants that can be used as plant-derived extracts include, for example, arch choke, almond, eye, Aisenhardia polystakia, eyebright, aonoryuzetsuran, aomoji, acacia concinna, acacia senegal, acacia senegal gum, acacia declins, red vine, etc. Akaharnile, Akayaziou, Agi, Agrimonia eupatoria, Akebii, Asagaokakarakusa, Azami, Ashi, Ashitaba, Ajugatsuru Kestonica, Azuki, Asunaro, Asparagus, Asparasas linearis, Acerola, Asenyaku, Asenyakunoki, Atlas Gace Sleiocarps, Avenastrigosa, Avocado, Ama, Amacha, Jiaogulan, Amadokoro, Amamo, Amiris balsamifera, American Psychic, American Sansho, American Showma, American Carrot, Amomu Aromachikum, Arakashi, Arcana, Arctium Majus , Arctium minus, Arnica, Arnica camisonis, Alpinia officinalum, Alfalfa (also known as purple vine), Aloe vera, Anzanju, Anzu, Angelica, Antilis brunellaria, Amperopsis grosedentata, Ammaroku, Ikarisou, Izayoibara, Itadori, Italian Strawberry, Strawberry, Strawberry, Ichiyakuso, Ginkgo, Itlan, Inagomame, Ibukitranoo, Imosemiru, Irakusa, Iran Iran, Iris, Iris Parida, Iwabenkei, Iwamitsuba, Ingenmame, Fennel, Witania Somnifera Utsubogusa, Udo, Umikuroumedoki, Ume, Uyaku, Uralkanzo, Uri, Urbarakutsuka, Urumusudavidiana, Uncariatomentosa, Unshuumikan, Agetsu, Eirantai, Edelweiss, Ezokogi, Ezosnake Strawberry, Ezomisohagi Ebine, Everyco, Elm,

Elemi, Enju, Peas, Passionflower, Passionflower, Passionflower, Ouren, Oazami, Passionflower, Oezodenda, Passionflower, Orchisma clata, Orchis maskra, Orchid, Oak (also known as European oak), Oakruma, Obana Oobanakakaonoki, Oobana salsbury, Oobeni mikan, Omitengu palm, Ominotokeisou, Omugi, All spice, Okazeri, Okura, Oshiroibana, Otaneninjin, Otogirisou, Onigeshi, Onisalvia, Onihamadaikon Passionflower, Dutch oak, olive, origanum heracleoticum, orthosihon stamineus, ormenis multicauris, orange, orobankerapum, gardenia tahitensis, carnation, kayasenegalensis, cabbage, cabbage, cassia, cashew nut, cabbage Sagrada, Cassiaitarika, Kappaficus alvarez, Cattleya, Canada Hydrastis, Canaryanoki, Kaninabara, Kanokosou, Kabaanatake, Cub, Gama (Kamo), Chamomile (also known as Chamomile), Kamimebouki, Kayupte, Karakusakeman , Galana, Garikabara, Califlower, Karin, Karuna (also known as Gyoryumodoki), Garsina Cambodia, Karrot, Kawarayomogi (also known as Passionflower), Passionflower, Passionflower, Passionflower, Kiwi, Kikarasuuri, Kikunigana, Kigeria Africana, Kidachiaroe, Kidachihakka , Kinoa, Kihada (also known as Oubaku), Kibanasushiro, Kibananohauchiwamame, Kibi, Gymnema, Kimokuren, Cassaba, Cabbage, Caraway, Cucumber (also known as Passionflower), Passionflower, Passionflower, Gyoryubai, Kiraya, Kiraya ), Gua, Guava, Quinceseed, Quercus Alba, Kukuinoki, Kuco, Kusanoou, Kudzu, Kusunoki, Passionflower, Kuchinashi, Kumakokemomo, Kumazasa, Verbena,

Kumanogiku, Kumin, Clameria Triandra, Clara, Granberry, Chrismum Marichimum, Grindeli Lobusta, Clintonia Borealis, Carbassaw, Walnut, Grapefruit, Clematis, Black Mustard, Black Mustard, Black Mustard, Gloves, Black Mustard, Japanese Comfrey, Japanese Comfrey, Japanese Comfrey , Kay (also known as cinnamon), comfrey, comfrey, cape aloe, keshi, gekabijin, bay, laurel, chemiyama kozorina, gentiana, gentiana prostrata, genno shoko, kemponashi, kouki, koukitan, kokeiten, kousuigaya Koganebana, Kokusina Indica, Kokutan, Cocrearia officinalis, Kokemomo, Coco palm, Gosho strawberry, Kosho, Koshiro no Sendangusa, Cochinil cactus, Kochosekoku, Kochoran, Konara, Kohakobe, Gobo, Sesame, Comfrey, Comfrey Barbatus, Gorenshi, Koroha, Conzlango, Comfrey (also known as Comfrey), Combretum Micransum, Saizarasa, Saishin, Cyperus esculentas, Cypress, Saiyousou, Southrea Involcrata, Sakishima Buttonzuru, Sakura, Sakura Bakanboku, Pomegranate , Sato maple, sugar cane, sugar pine, southern ka, sassa frassnoki, saffron, sabonsou, salasouju, salix nigra, salsbury, sanguinaria canadensis, sanzashi, sanshikisumire, sanshichininjin, sanshuyu, sansho, sanpens Arakunoidea, Shea Butterfly, Shiozakisou, Sicinburium Irio, Sistrose (also known as Labdanum), Sistus monsperiensis, Sistus sladanifers, Siso, Shichihenge, Shinanoki, Shibamugi, Gypterix odorata, Siberian fir, Simulva, Simi Comfrey, Comfrey, Potato, Shakyak, Jasmine, Comfrey, Janome Erica, Shukusha, Juzudama, Shukkonkasumiso,

Junipels Mexicana, Junsai, Ginger, Cardamom (also known as Cardamom), Shobu, Shoyodaiou, Shirakaba, Silane, Rogarashi, Shirobana Indosokei, Shirobana Rupin, Shirobanawata, Shinkonakarisaya, Synsepalm Durcificum, Cymbidium , Sweet acacia, watermelon, watermelon, scabiosa albensis, sugina, scuteraria galeri crater, stevia, strobe pine, spiny bamboo, spirulina platensis, spirulina maxima, suberihiyu, spert wheat, cymbidium cherry, smirax aristolocia ehoria, Peppermint, Cymbidium, Peppermint, Cardamom, Cymbidium, Peppermint, Cymbidium, Peppermint, Cymbidium, Peppermint, Cymbidium, Peppermint, Cymbidium, Peppermint White yanagi, dandelion, sage, star anise, star anise, sage, sage, sage, sage, sage, sage, sage, sage, sage, sage, sage, sage, sage, sage, sage, sage Cardamom, Star Anise, Cardamom, Elderberry, Elderberry, Ceylon Nikkei, Sage, Secropia obtusifolia, Sequoia osgi, Cecil Oak, Sedum Purpreum, Xenia Oy, Senega, Geranium, Cymbidium Floridam, Celeus Grandiflorus, Celery, Senkyu, Sentifolia rose, Sentipedakunningami, Senninkoku, Sempukuka, Senburi, Sojutsu, Souhakuhi, Soupalmet, Sokei, Soba, Soranumuricocarpum, Daiuikyo, Daikon, Daisanchiku, Soybean, Taiso, Daidai Thyme, Taiwan Hinoki, Takasaburo, Tachijakousou, Tachibana, Tabunoki, Tabebuia aberanedae, Tamasaki Tsuzurafuji, Damask rose, Onion, Damiana, Tarragon, Tanjin,

Tambolissa trichophylla, chigaya, cha (brown), chabotokeiso, tuberose, chouji, schisandra, chow bean, chili atmentosa, tsukushimenamomitsubaki, tsubokusa, tsurugumi, tsurudokudami, tsurureishi, tea tree, dioscorea compositor, dioscore Abilosa, Dioscorea Mexicana, Teuchigurumi, Tecoma clearis, Tetsuzainoki, Duke, Duboisia serrata, Terminaria, Tencha, German Ayame, German Tohi, Tougashi, Togarashi, Touki, Tokinsenka, Tounin, Tounezumimochi Corn, corn silk, Norway spruce, Tokusa, Houttuynia cordata, Houttuynia cordata, Toshishi (also known as Mamedaoshi), Tosho, Tomato, Trigonella foenum, Tormentilla, Tororoaoimodoki, Nagabagishigishi, Nagiikada, Nazuna, Natamame, Natsushiro Natsume, Natsume palm, Nankyosou, Nanbanai, Nanbankafuji, Nioisumire, Nioitenjikuaoi, Nioihiba, Nigayomogi, Nutmeg, Nigerasachiba, Nyukouju, Niranegi, Carrot, Ninnik, Ninfaea Alba, Nena Noborogiku, Pineapple, Hibiscus, Himatsu, Baobab, Bakuhosia Citriodora, Bakumondou, Bacopamonniera, Hagoromogusa, Hass, Parsley, Pachori, Hakkacreishi, Baccaris genisteroides, Passiflora Arata, Hatomugi,

Banana, Hanahakka, Honeysuckle, Vanilla, Vanilla Tahitensis, Hanesenna, Papaya, Pafiopedilum maudiae, Havelrea rodopensis, Hamanas, Hamamelis, Hayatouri, Rose, Parietaria, Harienju, Harpagofitum, Palmarosa Bambara groundnut, beet, chickpea, chickpea, hikiokoshi, bisna gabella, bitter almond, bitter orange, hydrastis canadensis, hinagiku, hinageshi, binan kazura, pinus heda, hinoki, hibamata, hihiragigiku, hippofaeram Himetsurunichinichisou, Himenaeakurubariru, Himefuuro, Byakudan, Chickpea, Hiramame, Comfrey, Biroudoaoi, Pyrocalps pennachihorius, Biwa, Binrou, Fafia paniculata, Fusenkazura, Pueraria mirifika Comfrey, Fusasuguri, Fusafuji Utsugi, Fusanus spicatus, Petit Glen, Petit Copetalum oracoides, Bussouge, Butcher Bloom, Pterocarps Marspium, Grape, Futomomo, Beech, Fuyuzansho, Fuyubodaiju, Fuyumusinatsukusatake Tyroensis, Brazilian nut tree, French kaigan show, French lavender, plantago afra, plantago ovata, plantagopsillium, primula shikimensis, prune, purnus africana, purnus serotina, plumeria, plectrantus barbatas, brechia hia cinchina , Propolis,

Bay, Hayflower, Hazelnut, Ponkan, Bechibel, Hechima, Pennyroyal Mint, Beninoki, Benibana, Benibana Assembly, Pepo Pumpkin, Hera Obako, Pelargonium Capitatum, Helichrysum Arenalium, Helichrysum Angustihorium, Helichrysum Italicum Bergamot, Perutoholmda silatis, Peruvian Balsam, Bergamot, Belberis Aquihorium, Veronica Ficinaris, Henna, Henruda, Houkigi, Boshuboku, Bowshunka, Hosenka, Horaisida, Horensou, Hawkweed, Hoonoki, Hokubeifuuroso , Boswellia Serata, Hosoba Senna, Hosoba Barengiku, Bodaiju, Button, Hop, Poterium Officinale, Hojoba, Polygonatum multiflorum, Bold, Ponkan, Macademia, Magnolia Officinalis, Magnolia Bionji, Magwa, Magnolia , Matsurika, Matecha, Madonna Lily, Mayorana, Maria Azami, Marpigia Grabula, Marumero, Maronie, Mango, Mangostin, Manshuakajika, Manshuakamatsu, Manjugiku, Mandarina Orange, Mandrake, Mishima Psycho, Mizuhakka, Mizulemon , Mitra Carpas Scaver, Mimosa Tenui Flora, Mirciaria Duvia, Mirra (also known as Motsuyakuju), Mirotamnus Flaberifolia, Mirobaran, Mukuroji, Murasaki, Murasakikibu, Murasaki Senburi, Murasaki Barengiku, Murayako Enji, Mebouki, Evening Primrose , Melissa (also known as Kousuihakka), Melirot, Melosria, Melon, Menta Albensis, Evening Primrose, Mourela Frubiachiris, Mokuren, Moscatabara, Moss Bean, Motsuyakuju, Momo, Momotamana, Yakumosou, Yagurumagiku, Yashabushi, Yachiyana

Yanagihakka, Yanagiran, Yamaguwa, Yamazakura, Yamanoimo, Yamayomogi, Yugao, Eucalyptus, Eupatorium ayapana, Eupatorium rebaudianum bertoni, Yukinoshita, Yuzu, Yusoboku, Yucha, Yukkagrauka, Yukkashijigera Lagenaria siceraria, Pokeweed, European Akinokirinsou, European strawberry, European green, European black laurel, European white hippo, European beech, European mannengusa, European fir, yomogi, yomogigiku, lychee (also known as lychee), lychee, lime, limegi, lilac , Radiata pine, latania, lychee, ranunculus ficaria, lavandula hybrida, rafuma, lavender, lalix europaea, larea divaricata, larea mexicana, rambutan, lychee, lychee, lychee glutinosa, ryugan, lilio Sumaobataki, apple, louchee, lupinus bucarnosus, rubusubirosus, rumpuyan, rurigisa, rurichenso, lespedesa, resedalteora, lettuce, redama, redam pulse tore, lebanon sugi, levis chikum officinale, lemon, lemongrass , Rosemary, Rosemary, Lowbush Blueberry, Roman Chamomile, Laurel (also known as Laurel), Logwood, Lobsta Coffee Tree, Wild Time, Wasabi, Wasabi Daikon, Wasabi Tree, Watasugigiku, Watafuji Utsugi, Walteria Indica, Waremokou, etc.

Ascofilm Nodosum, Aname, Oukimo, Okinawamozuku, Gigarchina Stellata, Kugermaniera Girata, Sargassum Filipendura, Sargassum Fushiform, Sargassum Mutikam, Sfaceraria Scoparia, Durbilea Antartica, Padina Pavonica, Himantaria elongata, fucas seratsus, pervecia canaliculata, macrocystis spirulina, mitsushikonbu, laminaria ochlorosquid, laminaria crowstoni, laminaria digitata, laminaria hiperbolea, wakame, asparagopsis algae , Catamen Kirinsai, Geridium Curtilagineum, Saimi, Shimatengusa, Darus, Chinorimo, Tochaka, Porphyridium cruentum, Marubachishimakuronori, Lithosamnium Coralioides, Anaaosa, Chlorella emersoni, Chlorella pyrenoidosa, Dunariella salina , Duna Liera Badawill, Hira Aonori, Spirulina Platensis, Spirulina Maxima, Haslea Ostrearia, Deleceria sanguinea, Pikea Lobusta, Pruuro Crisis Cartere, Hematococcus Prubialis, Orchid Algae, Condor Scrisps, Coral, Sea Whip, Red Algae such as algae;

Examples include lichens and hyphae such as Ebernia full fracea, Oakmoss, Usnia barbata, Cynomorium cocci, Kabaanatake, Shiitake, Ningyotake, Himematsutake, Fuyumusinatsukusatake, Kabaanatake, Choreimaitake, and winter worm summer grass.

The plant-derived extract may contain one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally 0.0001 to 5% by mass, preferably 0.0001 to 5% by mass, based on the total amount of the cosmetic composition. More preferably, it is 001 to 1% by mass.

Examples of animal-derived extracts include cow placenta extract, cow apical ligament (elastin) extract, cow blood (hematin) extract, cow / pig stomach (mucopolysaccharide) extract, cow / pig skin / fish (collagen). Examples include extract, chicken crown extract, bee royal milk (royal jelly), silk (fibroin, sericin) extract, milk (whey, sugar protein, lactoferrin) extract, crab shell (chitin, chitosan) extract, ceramide, squalene and the like.

The animal-derived extract may contain one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, 0.0001 to 5% by mass is preferable with respect to the total amount of the cosmetic composition, and 0.001. ~ 1% by mass is more preferable.

Examples of the microbial-derived extract include bacterial metabolites, bacterial extract extracts, metabolites such as mold and mushrooms, actinomycete metabolites, extracts such as mold and mushrooms, actinomycete extract, natto fungus metabolites, and natto extract. , Rice fermented extract, rice bran (red bran, white bran) fermented extract, Euglena extract or its decomposition product or its water-soluble derivative, lactic acid fermented product of raw milk or defatted milk powder, trehalose or its derivative and the like. In addition, the microorganism-derived extract may contain, for example, lipopolysaccharide (LPS).

The above-mentioned one or more kinds of microbial-derived extracts may be blended. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, 0.0001 to 5% by mass is preferable with respect to the total amount of the cosmetic composition, and 0.001. ~ 1% by mass is more preferable.

Examples of zinc compounds include zinc acetate, zinc laurate, zinc myristate, zinc palmitate, zinc ricinoleate, zinc undesylene, zinc aspartate, zinc acetylmethionine, zinc gluconate, zinc citrate, and polypyrrolidone carboxylic acid. Organic acid zinc salts such as zinc and zinc picolinate; zinc sulfonates such as zinc p-phenol sulfonate, zinc phosphate esters such as zinc ascorbyl phosphate, sodium cetyl phosphate, and DNA zinc; zinc complexes such as zinc pyrithione. Zinc-bearing zeolite such as (silver / zinc / ammonium) zeolite, silicic acid (ammonium / silver / zinc / aluminum); aluminum hydroxide such as aluminum oxide / zinc, aluminum oxide / zinc / cerium, aluminum oxide / zinc / iron Bake products such as: Zinc sulfate, Zinc sulfide, Zinc chloride, Zinc bromide, Zinc nitrate, Zinc ammonium chloride, Zinc aluminum sulfate, Zinc potassium sulfate, Zinc iodide, Basic zinc carbonate and other inorganic zinc salts; / Carbonate) (Mg / Al / Zinc), zinc oxide and the like. Among these, a compound that easily dissolves in water to form zinc ions is preferable, the solubility in 100 g of water at 20 ° C. is preferably 5 g or more, and the solubility is more preferably 10 g or more. Examples of such zinc compounds include zinc sulfate, zinc chloride, zinc gluconate, zinc bromide, zinc nitrate, zinc ammonium chloride, zinc aluminum sulfate, potassium zinc sulfate, zinc iodide and the like.

The zinc compound may contain one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.001 to 10% by mass with respect to the total amount of the cosmetic composition. 01 to 3% by mass is more preferable.

Examples of the thickener include dimethyldiallylammonium chloride / acrylamide copolymer, acrylamide / acrylic acid / dimethyldiallylammonium chloride copolymer, cellulose or a derivative thereof, keratin and collagen or a derivative thereof, calcium alginate, purulan, agar. , Gelatin, tamarind seed polysaccharide, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, arabic rubber, crystalline cellulose, arabinogalactan, karaya gum, tragacanth gum, alginic acid, albumin, casein, curdlan, β-1,3 Examples thereof include β-glucan other than -1,6-glucan, β-glucan derivative, gellan gum, dextran, α-glucose and α-glucose derivative, glucomannan, galactomannan, arabinoxylan, cellulose derivative, dextrin and dextrin derivative.

The thickener may be one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.01 to 20% by mass with respect to the total amount of the cosmetic composition. More preferably, it is 05 to 10% by mass.

Examples of the oil agent include volatile and non-volatile oil agents, solvents and resins usually used in cosmetic compositions, which may be liquids, pastes or solids at room temperature, but liquids having excellent handling are preferable. Examples of the oil agent include higher alcohols such as cetyl alcohol, isostearyl alcohol, lauryl alcohol, hexadecyl alcohol, and octyldodecanol; lauric acid, undecylene acid, myristic acid, palmitic acid, stearic acid, behenic acid, isostearic acid, 12 -Higher fatty acids such as hydroxystearic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, isostearic acid, 12-hydroxystearic acid, lanolin fatty acids; myristyl myristate, hexyl laurate, oleic acid Decyl, isopropyl myristate, hexyldecyl dimethyloctanoate, glycerin monostearate, diethyl phthalate, ethylene glycol monostearate, octyl oxystearate, diisopropyl adipate, cetyl octanate, isononyl isononanoate, isopropyl palmitate, stearic acid Stearyl, isotridecyl myristate, 2-ethylhexyl palmitate, octyldodecyl ricinolate, fatty acid cholesteryl / lanosteryl with 10-30 carbon atoms, cetyl lactate, lanolin acetate, ethylene glycol di-2-ethylhexanoate, pentaerythritol Estares such as fatty acid ester, dipentaerythritol fatty acid ester, cetyl caprate, glyceryl tricaprylate, diisostearyl malate, dioctyl succinate, and cetyl 2-ethylhexanoate; hydrocarbons such as liquid paraffin, vaseline, squalane; Rows such as lanolin, reduced lanolin, carnauba wax; fats and oils such as mink oil, cacao butter, palm oil, palm kernel oil, camellia oil, sesame oil, castor oil, olive oil; dimethylpolysiloxane, methylhydrozinepolysiloxane, methylphenylpolysiloxane , Polyether-modified organopolysiloxane, Fluoroalkyl-polyoxyalkylene co-modified organopolysiloxane, Alkyl-modified organopolysiloxane, Terminal-modified organopolysiloxane, Fluorine-modified organopolysiloxane, Amodimethicone, Amino-modified organopolysiloxane, Silicone gel, Acrylic Silicone compounds such as silicone, trimethylsiloxysilicate, silicone RTV rubber; fluorine compounds such as perfluoropolyether, fluoride pitch, fluorocarbon, fluoroalcohol; polybutene, polyisobutene, hydrogenated polyi Polyolefins or olefin oligomers such as sobutene, ethylene / α-olefin / co-oligomer; hydrocarbons such as mineral oil, light liquid isoparaffin, petrolatum, squalane and the like can be mentioned.

The oil agent may be a mixture of one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.1 to 90% by mass with respect to the total amount of the cosmetic composition. More preferably, it is 5 to 70% by mass.

Examples of the pigment include pigments such as Red No. 201, Yellow No. 4, Blue No. 1, Black No. 401, and rake pigments such as Yellow No. 4 Al Lake and Yellow No. 203 Ba Lake.

Examples of the pigment include white pigments such as titanium oxide, extender pigments such as silica, talc, mica, sericite and kaolin, and pearl pigments such as mica titanium. The shape of these pigments (spherical, rod-shaped, needle-shaped, plate-shaped, indefinite-shaped, scaly-shaped, spindle-shaped, etc.) is not particularly limited. These pigments have conventionally known surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil agent treatment, N-acylated lysine treatment, and polyacrylic. The surface may be treated in advance by acid treatment, metal soap treatment, amino acid treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment or the like. Moreover, you may use these as an ultraviolet scattering agent.

The pigment and / or the pigment may be blended with one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.1 to 90% by mass with respect to the total amount of the cosmetic composition. More preferably, it is 5 to 70% by mass.

Examples of the antibacterial agent (preservative) include paraben antibacterial agents such as methylparaben and ethylparaben; imidazole antibacterial agents such as thiabendazole and methylpriventol 2-benzimidazolylcarbamate; trichlorocarbanilide, cloflucarban and the like. Carvanilide antibacterial agents; thiazole antibacterial agents such as benzothiazole; triazine antibacterial agents such as debuconazole and cabinone; biguanide antibacterial agents such as chlorhexidine hydrochloride and polyhexamethylene biguanide, and ether derivative antibacterial agents such as phenoxyethanol and ethylhexylglycerin. , Ethylene glycol, diethylene glycol, triethylene glycol, butylene glycol, propylene glycol, dipropylene glycol, pentylene glycol, caprylyl glycol and other glucol antibacterial agents, sodium benzoate, potassium benzoate, calcium benzoate and other benzoic acids Antibacterial agents and the like can be mentioned. As the antibacterial agent, an ether derivative antibacterial agent, a glycol antibacterial agent, or the like is preferably used from the viewpoint of exerting the effect of the present invention.

The antibacterial agent may be one or more of the above, but when these antibacterial agents are used in combination, it should be used carefully in consideration of irritation to the human body. From the viewpoint of exerting the effect of the present invention, the blending amount thereof is preferably 0.01 to 10% by mass, more preferably 0.03 to 6% by mass, and 0.03 to 3% by mass with respect to the total amount of the cosmetic composition. % Is more preferable.

Examples of the pH adjuster include potassium carbonate, sodium hydrogen carbonate, ammonium hydrogen carbonate, ascorbic acid, hydrochloric acid, gluconic acid, acetic acid, lactic acid, phosphoric acid, sulfuric acid, citric acid and the like.

The pH adjuster may contain one or more of the above. The blending amount thereof is not particularly limited and varies depending on the type of the cosmetic composition, but from the viewpoint of exerting the effect of the present invention, the cosmetic composition is preferably pH 3.0 to 13.0, more preferably pH 4. It is preferable to add the mixture so as to have a pH of 0 to 12.0, more preferably pH 5.0 to 10.0, and most preferably pH 6.0 to 7.0. Further, the blending amount of the pH adjuster is generally preferably 0.001 to 10% by mass, more preferably 0.01 to 5% by mass, based on the total amount of the cosmetic composition, from the viewpoint of exerting the effect of the present invention.

Examples of the antioxidant include tocopherol, butylhydroxyanisole, dibutylhydroxytoluene, phytic acid and the like.
As the antioxidant, one or more of the above may be blended. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.001 to 10% by mass with respect to the total amount of the cosmetic composition. 01 to 5% by mass is more preferable.

Examples of the ultraviolet absorber include salicylic acid-based agents such as homomentyl salicylate, octyl salicylate, and triethanolamine salicylate; paraaminobenzoic acid, ethyldihydroxypropyl paraaminobenzoic acid, glyceryl paraaminobenzoic acid, octyldimethylparaaminobenzoic acid, and paradimethylaminobenzoic acid. PABAs such as amyl, 2-ethylhexyl paradimethylaminobenzoate; 4- (2-β-glucopyranosyloxy) propoxy-2-hydroxybenzophenone, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenone disulfonate, 2-hydroxy -4-methoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its trihydrate, sodium hydroxymethoxybenzophenone sulfonate, 2-hydroxy-4-methoxybenzophenone-5-sulfate, 2,2'-dihydroxy-4-methoxybenzophenone, 2 , 4-Dihydroxybenzophenone, 2,2'4,4'-tetrahydroxybenzophenone, 2,2'-dihydroxy-4,4'-dimethoxybenzophenone, 2-hydroxy-4-N-octoxybenzophenone and other benzophenones; 2-Ethylhexyl paramethoxycinerate (also known as octyl paramethoxycinerate), mono-2-ethylhexanate glyceryl diparamethoxycinerate, methyl 2,5-diisopropylsilicate, 2,4,6- Tris [4- (2-ethylhexyloxycarbonyl) anilino] -1,3,5-triazine, methylbis trimethoxycinerate (trimethylsiloxy) silylisopentyl, isopropyl diisopropylsilicate benzoate paramethoxycinerate Mixtures, silicic acids such as p-methoxyhydrosilicate diethanolamine salt; 2-phenyl-benzimidazole-5-sulfate, 4-isopropyldibenzoylmethane, 4-tert-butyl-4'-methoxydibenzoylmethane, etc. Benzophenone methane-based; 2-cyano-, 3-diphenylprop-2-enoic acid 2-ethylhexyl ester (also known as octocrylene), dimethoxybenzilidendioxoimidazolidine propionate 2-ethylhexyl, 1- (3,4-) Dimethoxyphenyl) -4,4-dimethyl-1,3-pentandione, synoxate, methyl-O-aminobenzophenone, 2-ethylhexyl-2-cyano-3,3-diphenylacrylle , 3- (4-Methylbenzylidene) camphor, octylriazone, 4- (3,4-dimethoxyphenylmethylene) -2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, high molecular weight derivatives of these. , And silane derivatives.

The ultraviolet absorber may be a mixture of one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.01 to 10% by mass with respect to the total amount of the cosmetic composition. 1 to 1% by mass is more preferable.

Examples of the moisturizing component include mucopolysaccharides such as deoxyribonucleic acid, hyaluronic acid and its sodium salt, potassium salt, and derivatives, chondroitin sulfate, collagen, elastin, chitin, chitosan, hydrolyzed eggshell membrane, polyoxyethylene methyl glucoside, and poly. Examples thereof include oxypropylene methyl glucoside, sodium lactate, urea, sodium pyrrolidone carboxylate, betaine, whey, ceramide, squalene, squalane and its derivatives, mevalonolactone and the like.

The moisturizing ingredient may be one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.001 to 30% by mass with respect to the total amount of the cosmetic composition, and 0. 01 to 20% by mass is more preferable.

Examples of enzymes include acylcoenzyme A desaturase, aminopeptidase, amyloglucosidase, oxidoreductase, catalase, glucose oxidase, superoxide dismutase, soybean peroxidase, dextran-immobilized protease, transglutaminase, hyaluronidase, protease, hesperidinase, lactoperoxidase, lipase. And so on.

The enzyme may contain one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.001 to 10% by mass with respect to the total amount of the cosmetic composition. 01 to 5% by mass is more preferable.

The fragrance is blended to add aroma and scent to the cosmetic composition or to mask an unpleasant odor. It is not particularly limited as long as it is a fragrance generally blended in a cosmetic composition, and flowers, seeds, leaves, roots, etc. of various plants including various extracts exemplified in the above-mentioned physiologically active ingredients and the like. Fragrances extracted from, fragrances extracted from seaweeds, fragrances extracted from animal parts or secretions (eg, jako, musk), artificially synthesized fragrances (eg, menthol, musk, acetate, vanilla) Is illustrated.

The fragrance may be a mixture of one or more of the above. The blending amount is not particularly limited and varies depending on the type of cosmetic composition, but from the viewpoint of exerting the effect of the present invention, it is generally preferably 0.001 to 10% by mass with respect to the total amount of the cosmetic composition. 01 to 3% by mass is more preferable.

The cosmetic composition of the present invention is, for example, a scalp care cosmetic; cream (hand cream, body cream), lotion (body lotion, sunscreen lotion, etc.), lotion, milky lotion, sunscreen milky lotion, etc. Liquids, beauty liquids used for face masks, beauty liquids used for sheet-shaped processed products (for example, sheets with fine needles), packs, shaving lotions, after-shave lotions, after-sun lotions and other skin care cosmetics; face wash Cleaning cosmetics such as materials, makeup removers, body shampoos; makeup cosmetics such as foundations, makeup bases, lip creams; can be used as bathing agents, among others, body lotions, body creams, lotions, lotions. , It is particularly preferable to use it as a milky lotion or a beauty liquid.

Next, a method for producing the cosmetic composition of the present invention will be described.
The cosmetic composition of the present invention can be produced according to a conventional method, and it is preferable that the above-mentioned yellowing improving agent is contained in the aqueous phase.

Next, a method of using the cosmetic composition containing the yellowing improving agent of the present invention will be described.
The method of using the yellowing improving agent of the present invention can be adjusted according to the condition, age, gender, etc. of the skin to be used. For example, when it is used as a cosmetic composition, the following method may be used. That is, several times a day (for example, about 1 to 5 times, preferably 1 to 3 times), an appropriate amount (for example, about 0.02 mg to 20 mg is preferably applied in a section of, for example, 6 cm × 10 cm). It can be applied to the skin of the whole body including the face and limbs. The application period is not particularly limited, but from the viewpoint of the skin turnover cycle and the efficient exertion of the effects of the present invention, for example, about 1 to 110 days is preferable, about 15 to 80 days is more preferable, and about 25 to 25 to 60 days is even more preferred.
The yellowing improving agent of the present invention can improve the yellowing of the skin. The target of use is not particularly limited, but it can be suitably used for people whose skin has turned yellow due to aging, stress, ultraviolet rays, excessive intake of sugar, lifestyle, and the like.

Next, the method for improving yellowing of the present invention will be described.
The method for improving yellowing of the present invention includes applying the cosmetic composition of the present invention to the skin. This can improve the yellowing of the skin. The application environment such as temperature of the cosmetic composition of the present invention is not particularly limited.

From the viewpoint of satisfactorily exerting the effect of improving the yellowing of the skin, β-1,3-1,6-glucan as a solid content, preferably 0.0001 to 1 mass, based on the total amount of the cosmetic composition. %, More preferably 0.0005 to 1% by mass, still more preferably 0.001 to 1% by mass, still more preferably 0.005 to 0.5% by mass, still more preferably 0.005 to 0.2% by mass. , Even more preferably 0.005 to 0.1% by mass, and most preferably 0.005 to 0.01% by mass. From the viewpoint of ease of preparation of the cosmetic composition and prevention of change in usability, β-1,3-1,6-glucan is contained in an amount of 1% by mass or less in terms of solid content with respect to the total amount of the cosmetic composition. Is preferable.

Hereinafter, the present invention will be specifically described with reference to Examples. In the following examples and the like, "%" is a "mass standard" unless otherwise specified.

<Examples 1 to 4 (preparation of samples A to D)>
Black yeast (deposit number FERM BP-8391) was cultured in potato dextrose agar slope medium to prepare a preserved strain, inoculated into a 500 ml Erlenmeyer flask containing 100 ml of YM liquid medium (manufactured by Diffco), and at 28 ° C. Pre-cultured for 3 days. This culture broth was transferred to a 30 liter fermenter containing 15 liters of Czapeak's medium (manufactured by Diffco) and cultured at 28 ° C. for 3 days. The culture solution was sterilized by heating at 90 ° C. for 30 minutes, and then the cells were removed by centrifugation to obtain a culture supernatant (β-1,3-1,6-glucan 1% by mass).

This culture supernatant was diluted to 0.8% with water and used as Sample A (Example 1) (β-1,3-1,6-glucan contained in Sample A was 0.008% by mass). ). Further, the culture supernatant was diluted to 80% with water and used as Sample B (Example 2) (β-1,3-1,6-glucan contained in Sample B was 0.8% by mass). ). Further, the culture supernatant was used as sample C (Example 3). Further, phenoxyethanol and ethylhexyl glycerin were added as antibacterial agents to the culture supernatant, and water was further added to dilute the culture supernatant to 80%, which was used as Sample D (Example 4) (Sample D). Β-1,3-1,6-glucan contained in is 0.8% by mass, phenoxyethanol is 0.5% by mass, and ethylhexylglycerin is 0.3% by mass).
The samples A to D correspond to the yellowing improving agent of the present invention.

The β-1,3-1,6-glucan contained in the samples A, B, C, and D has a mass average molecular weight of 300 in the β-1,3-1,6-glucan represented by the general formula (1). ^{The ratio of the unit whose R 1} is the formula (A) to the unit whose R ¹ is a hydrogen atom was 0.85. The method for measuring the mass average molecular weight and the method for calculating the unit ratio are shown below. In the general formula (1), 583 among a plurality of R ¹ is the formula (A), 686 among a plurality of R ¹ is a hydrogen atom.

(Measurement of mass average molecular weight)
Three times the amount of ethanol was added to the culture supernatant, cooled to −20 ° C. and left to stand for 10 minutes to obtain a precipitate. 5 mg of this β-1,3-1,6-glucan precipitate was taken in a tube, 1 ml of distilled water was added, and the mixture was dissolved in boiling water. This solution was passed through a 0.22 μm filter to prepare a sample for high performance liquid chromatography. For separation, a packed column KS-805, 804 (manufactured by Showa Denko KK) of Shodex, which is a high-speed liquid chromatograph gel filtration column, was used, and the flow velocity was 0.5 ml / min. , Temperature 80 ° C., RI detector for detection, and water as separation solvent. As a molecular weight marker, Shodex pullulan standard solution P-82 (manufactured by Showa Denko KK) was used, and the device constant k was determined and measured.

(Calculation of unit ratio)
For the analysis of the binding mode of β-1,3-1,6-glucan, methylation analysis was performed according to a conventional method. That is, the sample was dissolved in distilled water at a concentration of 1 mg / ml, three times the amount of ethanol was added to precipitate β-1,3-1,6-glucan, the supernatant was removed, and then the sample was reconstituted in distilled water. It was dissolved. This operation was repeated 3 times to prepare a purified sample from which components other than β-1,3-1,6-glucan were removed. 2 ml of DMSO was added to 1 mg of the lyophilized purified sample to dissolve it, and 0.5 ml of carbanion reagent was added. After stirring at room temperature for 4 _{hr, CH 3} I was added, and the mixture was stirred for 1 hr while cooling with water and reacted to methylate the free hydroxyl group. After _{spraying N 2} to remove excess CH ₃ I, the reaction solution was transferred to a dialysis tube and dialyzed against water. After the sample was dried, concentrated sulfuric acid (72%) was added, and the sample was subsequently diluted with water and acid hydrolyzed to obtain a monosaccharide. After completion of the reaction, the mixture _{was neutralized with BaCO 3} and the supernatant was added to an ion exchange resin for desalting. 2N Ammonia water was added to the sample, _{1 mg of NaBH 4} was added for reduction, and then 0.1 ml of acetic anhydride and pyridine were added and reacted for 2 hours to complete the acetylation of the hydroxyl group that contributed to the bond. The acetylated sample was dissolved in acetone and detected by gas chromatography. In addition, β-1,3-1,6-glucan was analyzed in detail using 13C-NMR. From these calculated values ^{, the ratio of the unit in which R 1} is the formula (A) to the unit in which ^{R 1} is a hydrogen atom was obtained.

<Measurement of skin color 1>
Samples A and B were applied to the inside of the upper left arm of 9 subjects twice a day, 2 ml each morning and evening for 4 weeks. The three sites on the inside of the upper left arm of each subject were set as test sites (1) to (3). Further, pullulan (manufactured by Hayashibara Co., Ltd., 90% by mass) (0.008% by mass aqueous solution) was similarly applied to another part inside the upper left arm of each subject. In each subject, the inside of the upper right arm where nothing was applied was used as the control. Before the start of application and 4 weeks after application, images of the test sites of Sample A (Example 1), Sample B (Example 2), Pullulan (Comparative Example 1), and Control (Comparative Example 2) are displayed in Antera 3D. ^The image was taken using TM (Mirabex). The skin color (b * value) at the test sites (1) to (3) was measured from the images taken using the Antera 3D ^{TM (Miravex) using the software attached to the aircraft.} The b * value is an index of yellowness, and the larger the value, the stronger the yellowness, and the smaller the value, the weaker the yellowness.

Table 1 shows the changes in the b * value of the subjects before the start of application and 4 weeks after application. The numerical values in Table 1 are the average values of the b * value measurements at the test sites (1) to (3) of the nine subjects.

From Table 1, 4 weeks after application, in the test sites (1) to (3), the b * values of the sample A and B coated sites were significantly lower than those of the non-coated sites and the pullulan coated sites. .. In addition, the p value indicates a significant difference (paired t-test) from before the start of application. The smaller the p-value, the more significant it was, and in particular, the p-value less than 0.05 was considered to be statistically significant. From the p-values, the b * values at the sample A and B-coated sites were more significant than those at the non-coated sites and the pullulan-coated sites after 4 weeks of application compared to before the start of application. From these, it was found that the yellowing was effectively improved by applying the samples A and B to the skin.

<Skin color measurement 2>
Cosmetic compositions (Examples 5 to 8, Comparative Examples 3 and 4) were prepared with the formulations shown in Table 2. The cosmetic compositions of Examples 5 to 8 and Comparative Examples 3 and 4 were applied by the same method as in the above-mentioned skin color measurement 1, and the b * value was measured. The results are shown in Table 3.

In the application sites of the cosmetic compositions of Examples 5 to 8, the b * value 4 weeks after the application was significantly higher than that before the start of application as compared with the application sites of the cosmetic compositions of Comparative Examples 3 and 4. It was declining.

Claims (9)

1. A yellowing improving agent containing β-1,3-1,6-glucan.
2. The yellowing improving agent according to claim 1, wherein β-1,3-1,6-glucan is represented by the following general formula (1).
   

   

   (In the formula (1), R ¹ represents a hydrogen atom or a group represented by the following formula (A), and ^{one or more of a plurality of R 1} is the following formula (A). A is the general formula (1). Represents a number having a mass average molecular weight of β-1,3-1,6-glucan represented by 3,000 to 5,000,000. Β-1,3-1, represented by the general formula (1). Both ends of 6-glucan are hydroxyl groups, and * represents a bond.)
   

   

   (In formula (A), * represents a bond.)
3. In the general formula (1), one or more of the plurality of R ¹ is the formula (A), 20 or more of the plurality of R ¹ is a hydrogen atom, yellow Gus seen improving agent according to claim 2.
4. The yellowing improving agent according to claim 2 or 3, wherein in the general formula (1), ^{the ratio of the unit in which R 1 is the formula (A) to the unit in which R 1} is a hydrogen atom is 0.2 to 1. ..
5. A cosmetic composition containing the yellowing-improving agent according to any one of claims 1 to 4, wherein β-1,3-1,6-glucan is used with respect to the total amount of the cosmetic composition. A cosmetic composition containing 0.0001 to 1% by mass.
6. The cosmetic composition according to claim 5, which contains 0.001 to 1% by mass as β-1,3-1,6-glucan with respect to the total amount of the cosmetic composition.
7. A method for improving yellowing, which comprises applying the cosmetic composition according to claim 5 or 6 to the skin.
8. Use of a yellowing dullness improving agent containing β-1,3-1,6-glucan or a cosmetic composition containing the yellowing dullness improving agent for improving yellowing.
9. Use of β-1,3-1,6-glucan for preparing a yellowing improving agent or for preparing a cosmetic composition containing the yellowing improving agent.