WO2021051349A1

WO2021051349A1 - Method for analysis of animal blood cells, analyzer, and storage medium

Info

Publication number: WO2021051349A1
Application number: PCT/CN2019/106669
Authority: WO
Inventors: 孔繁钢; 陈鹏震; 程蛟
Original assignee: 深圳迈瑞生物医疗电子股份有限公司
Priority date: 2019-09-19
Filing date: 2019-09-19
Publication date: 2021-03-25
Also published as: CN114174826A

Abstract

Disclosed is a method for the analysis of animal blood cells, applied to an animal blood cell analyzer (100). The method comprises: selecting a target measurement mode from various animal measurement modes, and determining a target dose of diluent according to the target measurement mode (401); mixing the target dose of diluent with the blood of an animal to be tested and performing a hemolysis treatment to obtain a detection sample (402); and testing the detection sample to obtain a white blood cell count and classification results (403). Also disclosed is an animal blood cell analyzer (100) and a storage medium.

Description

Animal blood cell analysis method, analyzer and storage medium

Technical field

The embodiment of the present invention relates to animal blood cell analysis technology, and relates to but is not limited to an animal blood cell analysis method, analyzer and storage medium.

Background technique

The main principle of animal blood cell analysis is to test the parameters of white blood cells, red blood cells and platelets on the diluted animal blood samples through micropores.

On an animal blood cell analyzer, blood samples of dogs, cats, rats, mice, rabbits and other animals need to be tested. However, the hemolysis process of the white blood cell channel of each animal is different. Therefore, in related technologies, an animal blood cell analyzer cannot guarantee the accuracy of the white blood cell count and classification results.

Summary of the invention

The embodiment of the present invention provides an animal blood cell analysis method, analyzer and storage medium to improve the accuracy of white blood cell count and classification results of different types of animals.

The embodiment of the present invention provides an animal blood cell analysis method, which is applied to an animal blood cell analyzer, and the method includes:

Select a target measurement mode from a variety of animal measurement modes, and determine the target dose of the diluent according to the target measurement mode;

Mixing the diluent of the target dose with the blood of the animal to be tested and performing hemolysis treatment to obtain a test sample;

The test sample is tested to obtain white blood cell count and classification results.

The embodiment of the present invention provides an animal blood cell analyzer. The animal blood cell analyzer includes:

The sampling needle assembly is used to suck the blood of the animal to be tested and send it to the first counting cell assembly;

The mode selection module is used to select a target measurement mode from a variety of animal measurement modes, and determine the target dose of the diluent according to the target measurement mode;

A reagent delivery component, which is used to mix the diluent of the target dose with the blood of the animal to be tested, and perform hemolysis treatment to obtain a test sample;

The first counting cell component is used to test the test sample to obtain the white blood cell count and classification results.

An embodiment of the present invention provides a storage medium with an executable program stored on the storage medium, and when the executable program is executed by a processor, the steps of the animal blood cell analysis method executed by the animal blood cell analyzer are implemented.

In the embodiment of the present invention, the target dose of the diluent used to dilute the animal’s blood is determined based on the animal measurement mode corresponding to the animal species, so that the target dose of the diluent is used to dilute the blood of the animal to be tested, so that the blood of the animal to be tested is diluted During the test, the blood of different kinds of animals is diluted with the corresponding dose of diluent to dilute the blood of the tested animal. By controlling the dosage of the diluent, the accuracy of the test results of lymphocytes and other white blood cells is improved.

Description of the drawings

FIG. 1 is a schematic diagram of the architecture of an optional animal blood cell analyzer provided by an embodiment of the present invention;

FIG. 2 is a schematic diagram of the principle of the impedance method counting test provided by an embodiment of the present invention;

3 is a schematic diagram of white blood cell curve distribution provided by an embodiment of the present invention;

Figure 4 is a schematic flow chart of an optional animal blood cell analysis method provided by an embodiment of the present invention;

5 is a schematic diagram of the architecture of an optional animal blood cell analyzer provided by an embodiment of the present invention;

Fig. 6 is a schematic flow chart of an optional animal blood cell analysis method provided by an embodiment of the present invention.

detailed description

The present invention will be further described in detail below in conjunction with the drawings and embodiments. It should be understood that the embodiments provided here are only used to explain the present invention, but not to limit the present invention. In addition, the embodiments provided below are part of the embodiments for implementing the present invention, rather than providing all the embodiments for implementing the present invention. In the case of no conflict, the technical solutions described in the embodiments of the present invention can be combined in any manner. Implement.

In various embodiments of the present invention: the animal blood cell analyzer selects a target measurement mode from a plurality of animal measurement modes, and determines the target dose of the diluent according to the target measurement mode; and changes the target dose of the diluent Mixing with the blood of the animal to be tested and performing hemolysis treatment to obtain a test sample; and testing the test sample to obtain white blood cell count and classification results.

The embodiment of the present invention provides an animal blood cell analysis method, which is applied to an animal blood cell analyzer. As shown in FIG. 1, the animal blood cell analyzer 100 includes: a sampling needle assembly 101, a mode selection module 102, and a reagent delivery assembly 103. The first counting pool component 104. Among them, the sampling needle assembly 101 may include a sampling needle and a syringe, which are used to draw blood from the animal to be tested and pump the drawn animal blood into the first counting cell assembly; the mode selection module 102 is processed by the animal blood cell analyzer The device is used to determine the current animal measurement mode. The reagent delivery component 103 is used to control the amount and time of reagents such as diluent and hemolytic agent entering the first counting cell component of the animal blood cell analyzer, and may include a syringe and a liquid path, wherein the liquid path is set to store the diluent and hemolysis Between the storage place for reagents and the first counting cell assembly, it is used to transfer reagents such as diluents and hemolytic reagents. The first counting cell component 104 can perform white blood cell classification and counting tests on blood samples of different types of animals in the first counting cell component.

The blood sample includes white blood cells, red blood cells, platelets, etc., which can be obtained by processing whole blood drawn from animals with diluents, hemolytic agents, and the like. Among them, the diluent is an isotonic liquid with appropriate ionic strength and conductivity with acid-base buffering effect. For example, the diluent contains hypoxanthine or xanthine compounds or their salts as the main component, or can also be other capable The diluent that plays the above-mentioned role. The role of hemolytic agents is to dissolve red blood cells for classification and counting of white blood cells. The hemolytic agent includes a surfactant, and specifically, it may include a cationic surfactant and a nonionic surfactant. Among them, the amount and concentration of the hemolytic agent can be selected reasonably according to the actual sample preparation requirements, and it is not specifically limited here. The hemolytic agent may contain a quaternary ammonium salt ion surfactant as a main component, or may also be any other surfactant capable of performing the above-mentioned functions.

Here, the sampling needle sends the blood of the animal to be tested into the first counting cell component, and the reagent delivery component adds the diluent and the hemolytic agent to the first counting cell component to obtain a blood sample, that is, the test sample. The first counting cell component counts the first The test sample in the pool assembly is the blood sample for testing.

The counting and classification of white blood cells can include three classifications, four classifications and five classifications. Taking the three classifications as an example, it means that white blood cells are divided into three major types, which are divided into small cell groups (cell groups composed of lymphocytes (Lymphocytes)) and intermediate cell groups (monocytes (Monocytes) through a certain dilution. The number of lymphocytes, monocytes and granulocytes in the blood sample is obtained. In the five classification, leukocytes can be directly classified into neutrophils, lymphocytes, eosinophils, basophils, and monocytes by means of a certain dilution and chemical staining or impedance method.

The first counting cell component can perform a white blood cell test on an animal blood sample through an impedance method counting test. The method of the first counting cell component performing impedance counting test on the detection sample in the first counting cell component may be as follows: the sampling needle draws a quantitative amount of animal blood to be tested from the blood sample, and injects it into the first counting cell component and The quantitative diluent is mixed uniformly to form a diluted test solution with a certain dilution ratio, the hemolytic agent is added, and after mixing, the impedance method counting test is performed in the first counting cell assembly to obtain the white blood cell channel counting result. Wherein, firstly, the test sample is obtained by diluting in the front pool of the first counting cell component, and then under the action of negative pressure, from the front cell to the back cell of the first counting cell component through the detection hole. During the counting process, the diluted sample flows from the front pool to the back pool to form a stable flow field. Since a constant current source is added between the positive electrode and the negative electrode, a stable electric field is formed, as shown in Figure 2. When blood cells pass through the detection hole 142, a pulse signal is formed, and particles are divided and counted by the pulse signal.

According to the principle of impedance method, after the hemolytic agent is treated, the red blood cells are ruptured, and the volume of the broken red blood cell fragments does not affect the counting of white blood cells. Under the action of the hemolytic agent, the white blood cells shrink in size and when white blood cells of different volumes pass through the small holes, the pulse size is different. Set the volume to 35-250 Fe liters (fl) of white blood cells. After treatment with hemolytic agent, lymphocytes are in the small cell area; granulocytes are in the large cell area, and monocytes are in the middle cell area. The animal blood cell analyzer calculates the percentage of cells in each subgroup according to the proportion of each subgroup in the total. Among them, Figure 3 is a schematic diagram of the three classifications of white blood cells, GOHST is red blood cell fragments, LYM is lymphocytes, MON is monocytes, and GRAN is neutrophils.

In the embodiment of the present invention, the first counting cell component does not impose any limitation on the number of classifications of the white blood cell classification of the test sample.

Of course, the embodiments of the present invention are not limited to the methods and hardware provided, and there may be multiple implementation manners, such as providing a storage medium (stored with programs or instructions for executing the animal blood cell analysis method provided by the embodiments of the present invention).

Fig. 4 is a schematic diagram of the implementation process of an animal blood cell analysis method provided by an embodiment of the present invention, as shown in Fig. 4, including:

S401: Select a target measurement mode from multiple animal measurement modes, and determine a target dose of the diluent according to the target measurement mode.

The mode selection module in the animal blood cell analyzer provides a variety of animal measurement modes, such as: rat measurement mode, cat measurement mode, dog measurement mode, etc., for example, animal measurement mode 1, animal measurement mode 2, etc. In an example, one type of animal measurement mode corresponds to one type of animal. For example, the animal type corresponding to the rat measurement mode is rat, the animal type corresponding to the cat measurement mode is cat, and the animal type corresponding to the dog measurement mode is dog. In another example, one animal measurement mode corresponds to multiple types of animals. For example, the animal types corresponding to the animal measurement mode 1 include rats and cats, and the animal types corresponding to the animal measurement mode 2 include dogs.

An animal measurement mode has a corresponding dose of diluent. Among them, the dosage of the diluent corresponding to different animal measurement modes can be the same or different, that is, the dosage of the diluent corresponding to different types of animals can be the same or different.

In practical applications, animal types with the same dosage of the corresponding diluent can correspond to the same animal measurement mode, and animal types with different dosages of the corresponding diluent correspond to different animal measurement modes. For example, animal types include: type A, type B, type C, and type D. Among them, the dose of the diluent corresponding to category A and C is dose 1, the dose of the diluent corresponding to category B is dose 2, and the dose of the diluent corresponding to category D is dose 3, then the animals corresponding to category A and category C The measurement mode is animal measurement mode a, the animal measurement mode corresponding to category B is animal measurement mode b, the animal measurement mode corresponding to category D is animal measurement mode c, and the dose of the diluent corresponding to animal measurement mode a is dose 1, animal The dose of diluent corresponding to measurement mode b is dose 2, and the dose of diluent corresponding to animal measurement mode c is dose 3.

The mode selection module in the animal blood cell analyzer determines the animal measurement mode corresponding to the blood of the animal to be tested based on the user's operation or the scanning of the scanner in the animal blood cell analyzer, and uses the determined dose of the diluent corresponding to the animal measurement mode as The current target dose is used to determine the current dose of the diluent that needs to be injected into the first counting cell assembly.

S402: Mix the target-dose diluent with the blood of the animal to be tested and perform hemolysis treatment to obtain a test sample.

After the mode selection module in the animal blood cell analyzer determines the target dose of the diluent, it sends the determined target calculation to the reagent delivery component, and the reagent delivery component mixes the target dose of the diluent with the animal's blood to be tested and performs Hemolysis treatment to obtain test samples. Here, the sampling needle assembly is used to suck the blood of the animal to be tested and send it to the first counting cell assembly.

The reagent delivery component mixes the hemolytic agent with the blood of the animal to be tested to perform hemolysis treatment on the blood of the animal to be tested, dissolve red blood cells in the blood of the animal to be tested, and retain white blood cells in the blood of the animal to be tested.

The sampling needle assembly sends the blood of the animal to be tested into the first counting cell assembly, and the reagent delivery assembly adds the target dose of diluent and hemolytic agent to the first counting cell assembly, and dilutes the blood of the animal to be tested in the first counting cell assembly And hemolysis treatment to obtain test samples. Optionally, the reagent delivery component adds the target dose of diluent to the first counting cell component at one time; optionally, the reagent delivery component adds the target dose of diluent to the first counting cell component in batches.

In an example, the animal blood cell analyzer is provided with a diluent storage area for storing diluents and a hemolytic agent storage area for storing hemolytic agents. The diluent storage area and the hemolytic agent storage area are respectively provided with a first counting cell assembly connected to the The liquid path, the reagent delivery component controls the inflow and stop of the inflow and stop of the diluent in the diluent storage area and the hemolyzing agent in the hemolytic agent storage area into the first counting cell component by controlling the opening and closing of the corresponding liquid path.

In one example, the animal blood cell analyzer is provided with a sampling needle that draws a certain amount of blood from the animal to be tested from a test tube containing the blood of the animal to be tested. When the sampling needle is controlled by the syringe, it is removed from the animal to be tested. After a certain amount of blood of the animal to be tested is drawn in the blood test tube, the sampling needle is moved to the detection area where the first counting cell assembly is located, and the syringe pumps the blood of the animal to be tested in the sampling needle into the first counting cell assembly, thereby removing the blood from the animal to be tested. The blood of the test animal is added to the first counting pool assembly. Here, the sampling needle can be moved to the top of the first counting cell assembly first. Before the syringe pumps the blood of the animal to be tested in the sampling needle into the first counting cell assembly, the sampling needle can extend vertically from above the first counting cell assembly. Move to the pool of the first counting cell assembly, and the syringe will inject the blood of the animal to be tested in the sampling needle in the pool into the first counting cell assembly, so as to prevent the blood of the animal to be tested in the sampling needle from splashing out of the first counting cell assembly .

For different target measurement modes, the amount of animal sample to be tested sucked by the sampling needle can be the same or different. In practical applications, the same animal blood cell analyzer, the sampling needle draws the same dose of the animal sample from the test tube, that is to say, the dose of the animal’s blood to be tested added to the first counting cell assembly is the same, so It is convenient to compare the test results of different kinds of animals.

In the embodiment of the present invention, the reagent delivery component may include one or more syringes, and the syringe used to add the diluent and hemolytic agent to the first counting cell component may be one syringe or different syringes. For example, the reagent delivery component includes a syringe through which the diluent and the hemolytic agent are respectively added to the first counting cell component. For another example, the reagent delivery component includes a first syringe and a second syringe, and the first syringe is used to add the diluent to the first counting cell component. The second syringe is used to add the hemolytic agent to the first counting cell assembly. In the embodiment of the present invention, there is no restriction on the number of syringes included in the reagent delivery assembly and the objects of the syringes.

In the embodiment of the present invention, the sampling needle assembly sends the blood of the animal to be tested into the first counting cell assembly and the sequence of adding the diluent and hemolytic agent to the first counting cell assembly by the reagent delivery assembly is not limited. For example, the sampling needle assembly and the reagent delivery assembly sequentially add diluent, animal blood and hemolytic agent to the first counting cell assembly. For another example: the sampling needle assembly and the reagent delivery assembly sequentially add diluent, hemolytic agent and animal blood to be tested into the first counting cell assembly. For another example: the sampling needle assembly and the reagent delivery assembly simultaneously add the diluent and the animal blood to be tested into the first counting cell assembly, and after adding the diluent and the animal blood to be tested, the reagent delivery assembly adds hemolysis to the first counting cell assembly Agent.

In practical applications, the diluent is added while adding the blood of the animal to be tested, and the sampling needle that draws the blood of the animal to be tested is cleaned by the diluent, so as to ensure that all the blood of the animal to be tested can be added to the sample needle. A counting cell assembly.

In one embodiment, the reagent delivery component of the animal blood cell analyzer empties the first counting cell component before mixing the diluent of the target dose with the blood of the animal to be tested and performing hemolysis treatment, so that the first counting The liquid in the pool assembly is cleaned up to avoid the influence of the remaining liquid in the first counting pool assembly on the test results of this test.

In one embodiment, the reagent delivery component of the animal blood cell analyzer mixes the diluent of the target dose with the blood of the animal to be tested and performs hemolysis. When the first counting cell component includes the diluent, the When testing at least two of animal blood and hemolytic agent, mix the liquid in the first counting cell assembly to fully react the diluent with the blood of the animal to be tested, the hemolytic agent and the blood of the animal to be tested, and ensure the test result Accuracy.

In the embodiment of the present invention, the dosage of the hemolytic agent added by the reagent delivery assembly to the first counting cell assembly can be adjusted according to the animal measurement mode, and the time of hemolysis treatment, that is, the reaction time between the hemolytic agent and the blood of the animal to be tested, can also be adjusted according to the animal The measurement mode is adjusted.

S403. Perform a test on the detection sample to obtain a white blood cell count and classification result.

The sampling needle assembly sends the blood of the animal to be tested into the first counting cell assembly, and the reagent delivery assembly adds the target dose of diluent and hemolytic agent to the first counting cell assembly to obtain the diluted and hemolyzed test sample, and analyze the animal blood cells The test result in the instrument tests the test sample in the first counting cell component to obtain the white blood cell count and classification result.

Optionally, after the test sample is obtained in the first counting cell component, the animal blood cell analyzer triggers the first counting cell component to test the test sample based on the user's operation. Optionally, after the test sample is obtained in the first counting cell component, the animal blood cell analyzer automatically triggers the first counting cell component to test the test sample. In an example, the animal blood cell analyzer automatically triggers the test of the test sample by the first counting cell component based on the reaction time of the hemolytic agent and the blood of the animal to be tested.

After the first counting cell component obtains the white blood cell count and classification results, it can send the white blood cell count and classification results to the display of the animal blood cell analyzer, and present it to the inspector through the display of the animal blood cell analyzer, and can also display the white blood cell count and classification results. The classification results are sent to the display of other equipment other than the animal blood cell analyzer, and presented to the inspector through the display of other equipment. Here, the animal blood cell analyzer and other equipment can be connected via wired or wireless methods.

In the embodiment of the present invention, the target dose of the diluent used to dilute the animal’s blood is determined based on the animal measurement mode corresponding to the animal species, so that the target dose of the diluent is used to dilute the blood of the animal to be tested, so that the blood of the animal to be tested is diluted During the test, the blood of different types of animals is diluted with the corresponding dose of diluent to dilute the blood of the animal to be tested. By controlling the dosage of the diluent, the accuracy of the test results of lymphocytes and other white blood cells is improved.

In the embodiment of the present invention, for different kinds of animals, the dilution and hemolysis treatment of the blood of the animal to be tested can be adjusted by adjusting the dosage of the diluent, the dosage of the hemolytic agent, and the reaction time of the hemolytic agent with the blood of the animal to be tested. Improve the accuracy of white blood cell classification and counting results of various animal blood.

Based on the animal blood cell analyzer shown in FIG. 1, the animal blood cell analyzer provided by the embodiment of the present invention may also include a display 105 and an input device 106 as shown in FIG. 5. Among them, the display 105 is used to display a software interface, and the input device 106 includes a keyboard or a mouse for the user to input information or instructions. In the embodiment of the present invention, the display 105 and the input device 106 may be integrated as a touch display, which can receive information or instructions input by the user while displaying the software interface.

In one embodiment, based on the animal blood cell analyzer shown in FIG. 5, S401 selects a target measurement mode from a variety of animal measurement modes, including: outputting an animal measurement mode selection interface; receiving an animal in the measurement mode selection interface The input operation or the selection operation of the measurement mode; the target measurement mode is determined from a plurality of animal measurement modes according to the input operation or the selection operation.

Here, the display 105 is used to output the animal measurement mode selection interface; the input device 106 is used to receive input operations or selection operations for the animal measurement mode in the measurement mode selection interface; the mode selection module 102 is used to The input operation or the selection operation determines the target measurement mode from a plurality of animal measurement modes.

The display can output an animal measurement mode selection interface for selecting an animal measurement mode. In the animal measurement mode, a variety of animal measurement modes or animal types can be displayed, so that the user can input the current animal measurement mode through the input device based on the animal measurement mode selection interface. Type or target measurement mode. When the information input by the user is the type of animal, the mode selection module can determine the animal measurement mode corresponding to the input animal type from multiple animal measurement modes as the target measurement mode.

The input device can receive the user's input operation or selection operation. When the input device receives the user's input operation, an input interface may be provided in the animal measurement mode selection interface, and the animal type or target measurement mode input by the user is received through the input interface. When the input device receives the user's input operation, a variety of animal types or animal measurement modes can be provided in the animal measurement mode selection interface, and the animal type or target measurement mode selected by the user is determined.

Based on the animal blood cell analyzer shown in FIG. 1, the animal blood cell analyzer provided by the embodiment of the present invention may also include a scanner 107 as shown in FIG. 5. Among them, the scanner 107 is used to scan information identifiers such as barcodes and two-dimensional codes to obtain the information carried by the information identifiers.

In one embodiment, based on the animal blood cell analyzer shown in FIG. 5, selecting a target measurement mode from a variety of animal measurement modes, including: scanning the information identifier on the test tube containing the blood of the animal to be tested to obtain the Sample information of the blood of the animal to be tested; according to the sample information, a target measurement mode is selected from a variety of animal measurement modes.

Here, the scanner 107 is used to scan the information identifier on the test tube containing the blood of the animal to be tested to obtain sample information of the blood of the animal to be tested; the mode selection module 102 is used to, according to the sample information, Select the target measurement mode from a variety of animal measurement modes.

The test tube containing the blood of the animal to be tested is affixed with a label, and the label contains a barcode, two-dimensional code and other information identifiers that carry the information of the animal’s blood to be tested. The information carried by the information identifier may include: the blood of the animal to be tested belongs to The type of animal, the Universally Unique Identifier (UUID) of the animal's blood to be tested, and the test item is waiting to test the blood sample information of the animal. In the embodiment of the present invention, the sample information carried by the information identifier is not limited in any way.

When the test tube containing the blood of the animal to be tested is within the scanning range of the scanner, the scanner scans the information mark on the test tube to obtain the sample information of the blood of the animal to be tested carried by the information mark, and then scan the scanned animal to be tested The blood sample information is sent to the mode selection module, and the mode selection module determines the animal measurement mode corresponding to the animal blood in the test tube according to the sample information related to the animal type or the animal measurement mode in the sample information scanned by the scanner, that is, the target measurement mode .

For example, if the sample information scanned by the scanner includes information that characterizes the animal measurement mode, and the animal measurement mode is a cat measurement mode, the mode selection module determines that the target measurement mode is a cat measurement mode. For another example: the sample information scanned by the scanner includes the animal species to which the blood of the animal to be tested belongs, and the animal species is cat, then the model selection module determines that the target measurement mode is the cat measurement mode. For another example, the sample information scanned by the scanner includes the UUID, which is the sample ID of the blood of the animal to be tested, and the sample ID is 1111. The mode selection module determines that the animal measurement mode corresponding to the blood of the animal to be tested is the cat measurement mode according to the sample ID 1111. , The target measurement mode is determined to be the cat measurement mode.

In the embodiment of the present invention, based on whether the diluent of the target dose is added to the first counting cell assembly at one time, in the embodiment of the present invention, the method of adding the diluent, the blood of the animal to be tested, and the hemolytic agent to the first counting cell assembly Can include:

Join method one, one-time join

In the first method of adding, S402 mixes the diluent of the target dose with the blood of the animal to be tested and performs hemolysis treatment to obtain a test sample, including: adding the diluent of the target dose to the first counting cell assembly; The hemolytic agent and the blood of the animal to be tested are added to the first counting cell component to obtain a test sample. Here, the sampling needle assembly sends the blood of the animal to be tested into the first counting pool assembly, and the reagent delivery assembly adds a target dose of diluent to the first counting pool assembly; and adds to the first counting pool assembly Hemolytic agent.

When the reagent delivery component adds the diluent to the first counting cell component, it directly adds the target dose of diluent to the first counting cell component, and after adding the target dose of diluent, adds and hemolyzes to the first counting cell component Agent. Wherein, the sampling needle can send the blood of the animal to be tested into the first counting cell component during or after the reagent delivery component adds the diluent to the first counting cell component. For example, the reagent delivery component adds the target dose of diluent to the first counting cell component, and after the reagent delivery component adds the target dose of diluent, the sampling needle component adds the blood of the animal to be tested to the first counting cell component, and then the sample After the needle assembly is added to the blood of the animal to be tested, the reagent delivery assembly continues to add the hemolytic agent to the first counting cell assembly. For another example, when the reagent delivery component adds the target dose of diluent to the first counting cell component, the sampling needle component adds the blood of the animal to be tested into the first counting cell component, and the sampling needle component adds the first counting cell component to the first counting cell component. After the blood of the animal to be tested, the reagent delivery component continues to add the hemolytic agent to the first counting cell component.

In the embodiment of the present invention, when the target dose of the diluent is added at one time, the process of adding the diluent is uninterrupted, but the order of adding the diluent, hemolytic agent, and animal blood to be tested is not limited in any way.

Join method two, join in by stages

In addition method 1, S402 mixing the target dose of diluent with the blood of the animal to be tested and performing hemolysis treatment includes: adding the blood of the animal to be tested to the first dose of diluent to obtain a reference test sample; A second dose of diluent and hemolytic agent are added to the reference test sample to obtain the test sample, and the sum of the first dose and the second dose is the target dose. Here, the reagent delivery component adds a first dose of diluent to the first counting cell before or while the sampling needle component sends the blood of the animal to be tested into the first counting cell; And after adding the blood of the animal to be tested into the first counting cell component, a second dose of diluent and the hemolytic agent are added to the first counting cell component.

The reagent delivery component first adds the first dose of diluent to the first counting cell component, and the sampling needle component adds the blood of the animal to be tested to the first counting cell component, and the animal to be tested is added to the first counting cell component in the sampling needle component. After the blood, the reagent delivery component continues to add the second dose of diluent to the first counting cell component, and adds the hemolytic agent, so that the first dose of diluent is added to the first counting cell component, and then to the first counting cell Add a second dose of diluent to the assembly.

Here, the reagent delivery component adding the first dose of diluent to the first counting cell component can be executed before the sampling needle component adds the blood of the animal to be tested to the first counting cell component, or it can be combined with the sampling needle component to the first counting. Adding the blood of the animal to be tested into the pool assembly is performed at the same time.

In the case where the dose of blood of the animal to be tested added in different animal measurement modes is the same, the size of the first dose may be the same or different.

In one embodiment, the animal blood cell analyzer draws a fixed dose of the reference test sample from the reference test sample before adding the second dose of diluent and hemolytic agent to the reference test sample; The fixed dose of the reference test sample is subjected to red blood cell test and/or platelet test. Here, the sampling needle assembly in the animal blood cell analyzer draws a fixed dose of a reference test sample composed of the first dose of diluent and the animal blood to be tested from the first counting cell assembly; the first The second counting cell component performs red blood cell test and/or platelet test on the reference test sample of the fixed dose.

The second counting cell component is a different counting cell component from the first counting cell component. Optionally, the second counting cell component and the first counting cell component belong to the same animal blood cell analyzer. Optionally, the second counting cell component and the first counting cell component belong to different animal blood cell analyzers.

Before adding the hemolytic agent to the first counting cell component to form a test sample, the first counting cell component only includes the first dose of diluent and the animal's blood to be tested, that is, the first counting cell component is for diluting the blood of the animal to be tested At this time, the reference test sample can be drawn from the first counting cell assembly by the sampling needle assembly, and the second counting cell assembly can perform at least one of the following red blood cell-related tests on the reference test sample drawn by the sampling needle: Red blood cell test and platelet test.

The dose of the reference test sample drawn by the sampling needle assembly from the first counting cell assembly, that is, the size of the fixed dose can be 9uL, 11uL, and so on. The size of the fixed dose can be set according to actual needs, which is not limited in the embodiment of the present invention.

In one embodiment, for different animal measurement modes, the first dose is the same.

For different animal measurement modes, that is, different animal species, the reference test sample has the same dose of the animal sample to be tested, and the same diluent dose, the dilution ratio of the reference test sample corresponding to different animal species is the same, so It is convenient to compare the red blood cell test or platelet test results of different animal species.

In the second addition method, based on whether the first dose of diluent is added to the first counting cell assembly at one time, the second addition method can include the following two situations:

Case 1: The first dose of diluent is added all at once

In case 1, adding the blood of the animal to be tested to the first dose of diluent to obtain a reference test sample includes: adding the first dose of diluent to the first counting cell assembly; The blood of the animal to be tested is added to the counting pool assembly to obtain a reference test sample. Here, the reagent delivery component adds the first dose of diluent to the first counting cell component before the sampling needle component sends the blood of the animal to be tested into the first counting cell component.

Case 2: The first dose of diluent is added in portions

In the second case, adding the blood of the animal to be tested to the first dose of diluent to obtain a reference test sample includes: adding a third dose of diluent to the first counting cell assembly; A fourth dose of diluent and the blood of the animal to be tested are added to the counting cell assembly to obtain a reference test sample, and the sum of the third dose and the fourth dose is the first dose. Here, the reagent delivery component adds a third dose of diluent to the first counting cell component to the first counting cell component before the sampling needle component sends the blood of the animal to be tested into the first counting cell component; and When the sampling needle assembly sends the blood of the animal to be tested into the first counting pool assembly, a fourth dose of diluent and the blood of the animal to be tested are added to the first counting pool assembly.

It should be noted that Case 1 and Case 2 are examples of adding the diluent of the target dose to the first counting cell assembly in two and three times. In practical applications, the diluent of the target dose is added to the first counting cell assembly. The number of times of adding in batches, and the order of adding the same hemolytic agent and blood to be tested can be controlled according to actual needs.

The reagent delivery assembly and the sampling needle assembly can simultaneously add a fourth dose of diluent and the blood of the animal to be tested into the first counting cell assembly, and the blood of the animal to be tested on the sampling needle is washed by the added diluent to ensure The blood of the animal to be tested can all be added to the first counting pool component to ensure the accuracy of classification and counting results.

In the animal blood cell analysis method provided by the embodiments of the present invention, the operator can flexibly set the target dose of diluent adding process according to actual needs. For example, when adding the target dose of diluent, first add the first counting cell assembly to the first counting cell assembly. Dose of diluent and animal blood to be tested, and then add a second dose of diluent and hemolytic agent to the first counting cell assembly, so that in the process of forming the test sample, a reference test sample can be obtained, which can be used for white blood cell testing at the same time , Carry out red blood cell test, platelet test and other tests related to red blood cells.

In the following, the animal blood cell analysis method provided by the embodiment of the present invention will be described through specific test scenarios, as shown in FIG. 6, including:

S601. The sampling needle assembly sucks blood of the animal to be tested.

The sampling needle assembly draws a specified dose of animal blood to be tested from the test tube.

S602. The first counting pool component is emptied.

S603: The reagent delivery component adds a first dose of diluent to the first counting cell component.

The syringe in the reagent delivery assembly opens the liquid path between the first counting cell assembly and the diluent storage position, adds the diluent to the first counting cell assembly through the opened liquid path, and after adding the first dose of diluent, the The opened hydraulic circuit is closed. During this process, the liquid path between the first counting cell component and the hemolytic agent storage position is in a closed state.

S604: The sampling needle assembly adds blood of the animal to be tested into the first counting cell assembly.

The syringe in the sampling needle assembly controls the sampling needle to move from the sampling area for sucking the blood of the animal to be tested from the test tube to above the first counting cell assembly in the detection area for detecting the blood sample, and from the first counting cell The upper part of the assembly moves into the pool of the first counting cell assembly, and the syringe in the sampling needle assembly injects the blood of the animal to be tested in the sampling needle in the first counting cell assembly into the first counting cell assembly.

S605, mix well.

Mix the diluent in the first counting cell assembly with the blood of the animal to be tested to obtain a reference test sample.

S606. The sampling needle assembly draws a reference test sample from the first counting cell assembly to perform red blood cell test and platelet test.

S607: The reagent delivery component adds a hemolytic agent to the first counting cell component.

The syringe in the reagent delivery assembly opens the liquid path between the first counting cell assembly and the hemolytic agent storage position, and adds the hemolytic agent to the first counting cell assembly through the opened liquid path. After adding the hemolytic agent, the opened liquid path is opened shut down. During this process, the liquid path between the first counting cell component and the diluent storage position is in a closed state.

S608: The reagent delivery component adds a second dose of diluent to the first counting cell component.

The process of adding the diluent into the first counting cell assembly by the reagent delivery assembly is the same as S603.

S609. Mix well.

Mix the diluent in the first counting cell assembly, the blood of the animal to be tested, and the hemolytic agent to obtain a test sample.

S610, the first counting cell component is tested.

The first counting cell component tests the reference sample in the first counting cell component to obtain white blood cell count and classification results.

The animal blood cell analysis method provided by the embodiment of the present invention uses different diluent dosages between different animal species when performing white blood cell channel test between different animal species, combined with adjusting the hemolysis dose and hemolysis time, so as to ensure the accuracy of the white blood cell count test result. Under the premise of sex, provide the accuracy of lymphocyte test results.

Before the animal blood cell analysis method provided by the embodiment of the present invention is used, the percentage of lymphocytes in the blood sample of the mouse is 65%. The blood sample is microscopically examined, and the percentage of lymphocytes in the microscopic examination result is 75%. The result of the microscopic examination is There is a 10% difference from the actual measurement result. When we test, we will find that it is not a sample, almost all samples have the same problem. By adjusting the amount of different hemolytic agents added, the reaction time will increase, and the lymphocyte count will be improved, but the inaccurate lymphocyte count cannot be completely resolved. The problem. The animal blood cell analysis method provided by the embodiment of the present invention can further improve the accuracy of the lymphocyte test result by changing the amount of the diluent.

Hereinafter, the test results of the animal blood cell analysis method provided in the embodiment of the present invention will be described through the count results of lymphocytes in blood samples of different types of animals.

The amount of blood collected in this embodiment can be 9ul. Before adjustment, all measurement modes correspond to 2.4ml diluent. Table 1 collected the blood of 7 horses to form the blood of 7 animals to be tested, and set the adjusted diluent volume to 1.4ml. The percentage of lymphocytes before the diluent volume was adjusted, and the diluent volume after the diluent volume was adjusted. The percentage of lymphocytes and the percentage of lymphocytes in the microscopic examination results are compared. Table 2 The blood of 7 mice was collected to form 7 animal blood to be tested, and the adjusted dilution volume was set to 1.6ml, the percentage of lymphocytes before the adjustment of the dilution volume, and the adjustment of the dilution volume The percentage of lymphocytes and the percentage of lymphocytes in the microscopic examination results are compared. Among them, Lym% represents the percentage of lymphocytes.

Table 1,

Table 2,

It can be seen from Table 1 and Table 2 that by adjusting the amount of blood sample diluent in the white blood cell count of different animals, the accuracy of the lymphocyte test result can be ensured on the basis of ensuring the accuracy of the white blood cell count result.

The embodiment of the present invention further provides a storage medium, that is, a computer-readable storage medium, and an executable program is stored on the storage medium. When the executable program is executed by a processor, the animal blood cell analyzer can be executed by the animal blood cell analyzer. Steps of blood cell analysis method.

The description of the above medium embodiment is similar to the description of the above method embodiment, and has similar beneficial effects as the method embodiment. For technical details not disclosed in the embodiment of the storage medium of the present invention, please refer to the description of the method embodiment of the present invention for understanding.

In the embodiment of the present invention, if the above animal blood cell analysis method is implemented in the form of a software functional module and sold or used as an independent product, it can also be stored in a computer readable storage medium. Based on this understanding, the technical solutions of the embodiments of the present invention can be embodied in the form of a software product in essence or a part that contributes to the prior art. The computer software product is stored in a storage medium and includes several instructions for A computer device (which may be a personal computer, a server, or a network device, etc.) executes all or part of the methods described in the various embodiments of the present invention. The aforementioned storage media include: U disk, mobile hard disk, Read Only Memory (ROM, Read Only Memory), magnetic disk or optical disk and other media that can store program codes. In this way, the embodiments of the present invention are not limited to any specific combination of hardware and software.

It should be understood that “one embodiment” or “an embodiment” mentioned throughout the specification means that a specific feature, structure, or characteristic related to the embodiment is included in at least one embodiment of the present invention. Therefore, the appearance of "in one embodiment" or "in an alternative embodiment" in various places throughout the specification does not necessarily refer to the same embodiment. In addition, these specific features, structures or characteristics can be combined in one or more embodiments in any suitable manner. It should be understood that, in various embodiments of the present invention, the size of the sequence numbers of the foregoing processes does not mean the order of execution. The execution order of the processes should be determined by their functions and internal logic, and should not be used in the embodiments of the present invention. The implementation process constitutes any limitation. The sequence numbers of the foregoing embodiments of the present invention are only for description, and do not represent the superiority of the embodiments.

It should be noted that in this article, the terms "include", "include" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, It also includes other elements not explicitly listed, or elements inherent to the process, method, article, or device. If there are no more restrictions, the element defined by the sentence "including a..." does not exclude the existence of other identical elements in the process, method, article, or device that includes the element.

In the several embodiments provided by the present invention, it should be understood that the disclosed device and method may be implemented in other ways. The device embodiments described above are merely illustrative. For example, the division of the units is only a logical function division, and there may be other divisions in actual implementation, such as: multiple units or components can be combined, or It can be integrated into another system, or some features can be ignored or not implemented. In addition, the coupling, or direct coupling, or communication connection between the components shown or discussed may be indirect coupling or communication connection through some interfaces, devices or units, and may be in electrical, mechanical or other forms. of.

The units described above as separate components may or may not be physically separate, and the components displayed as units may or may not be physical units; they may be located in one place or distributed on multiple network units; Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of the embodiment.

In addition, the functional units in the embodiments of the present invention can be all integrated into one processing unit, or each unit can be individually used as a unit, or two or more units can be integrated into one unit; the above-mentioned integration The unit can be implemented in the form of hardware, or in the form of hardware plus software functional units.

Those of ordinary skill in the art can understand that all or part of the steps in the above method embodiments can be implemented by a program instructing relevant hardware. The foregoing program can be stored in a computer readable storage medium. When the program is executed, the execution includes The steps of the foregoing method embodiment; and the foregoing storage medium includes: various media that can store program codes, such as a removable storage device, a read only memory (Read Only Memory, ROM), a magnetic disk, or an optical disk.

Alternatively, if the aforementioned integrated unit of the present invention is implemented in the form of a software function module and sold or used as an independent product, it can also be stored in a computer readable storage medium. Based on this understanding, the technical solutions of the embodiments of the present invention can be embodied in the form of a software product in essence or a part that contributes to the prior art. The computer software product is stored in a storage medium and includes several instructions for A computer device (which may be a personal computer, a server, or a network device, etc.) executes all or part of the methods described in the various embodiments of the present invention. The aforementioned storage media include: removable storage devices, ROMs, magnetic disks or optical discs and other media that can store program codes.

The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention. It should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims

An animal blood cell analysis method, applied to an animal blood cell analyzer, the method comprising:

Select a target measurement mode from a variety of animal measurement modes, and determine the target dose of the diluent according to the target measurement mode;

Mixing the diluent of the target dose with the blood of the animal to be tested and performing hemolysis treatment to obtain a test sample;

The test sample is tested to obtain white blood cell count and classification results.
The method according to claim 1, wherein selecting the target measurement mode from a plurality of animal measurement modes includes:

Output animal measurement mode selection interface;

Receiving an input operation or selection operation for the animal measurement mode in the measurement mode selection interface;

The target measurement mode is determined from a plurality of animal measurement modes according to the input operation or the selection operation.
The method according to claim 1, wherein selecting the target measurement mode from a plurality of animal measurement modes includes:

Scan the information identifier on the test tube containing the blood of the animal to be tested to obtain sample information of the blood of the animal to be tested;

According to the sample information, the target measurement mode is selected from a plurality of animal measurement modes.
The method according to claim 1, wherein mixing the diluent of the target dose with the blood of the animal to be tested and performing hemolysis treatment to obtain a test sample comprises:

Adding the diluent of the target dose to the first counting cell assembly;

The hemolytic agent and the blood of the animal to be tested are added to the first counting cell assembly to obtain a test sample.
The method according to claim 1, wherein the mixing the target dose of the diluent with the blood of the animal to be tested and performing hemolysis treatment comprises:

Adding the blood of the animal to be tested to the first dose of diluent to obtain a reference test sample;

A second dose of diluent and hemolytic agent are added to the reference test sample to obtain the test sample, and the sum of the first dose and the second dose is the target dose.
The method according to claim 5, wherein, before adding a second dose of diluent and hemolytic agent to the reference test sample, the method further comprises:

Drawing a fixed dose of the reference test sample from the reference test sample;

Red blood cell test and/or platelet test are performed on the reference test sample of the fixed dose.
The method according to claim 5 or 6, wherein the first dose is the same for different animal measurement modes.
The method according to claim 5, wherein the adding the blood of the test animal to the first dose of diluent to obtain a reference test sample comprises:

Adding the first dose of diluent to the first counting cell assembly;

The blood of the animal to be tested is added to the first counting cell assembly to obtain a reference test sample.
The method according to claim 5, wherein the adding the blood of the test animal to the first dose of diluent to obtain a reference test sample comprises:

Add a third dose of diluent to the first counting cell assembly;

A fourth dose of diluent and the blood of the animal to be tested are added to the first counting cell assembly to obtain a reference test sample, and the sum of the third dose and the fourth dose is the first dose.
An animal blood cell analyzer. The animal blood cell analyzer includes:

The sampling needle assembly is used to suck the blood of the animal to be tested and send it to the first counting cell assembly;

The mode selection module is used to select a target measurement mode from multiple animal measurement modes, and determine the target dose of the diluent according to the target measurement mode;

A reagent delivery component, which is used to mix the diluent of the target dose with the blood of the animal to be tested, and perform hemolysis treatment to obtain a test sample;

The first counting cell component is used to test the test sample to obtain the white blood cell count and classification results.
The animal blood cell analyzer according to claim 10, wherein the animal blood cell analyzer further comprises: a display and an input device;

The display is used to output an animal measurement mode selection interface;

The input device is configured to receive input operations or selection operations for the animal measurement mode in the measurement mode selection interface;

The mode selection module is configured to determine a target measurement mode from multiple animal measurement modes according to the input operation or the selection operation.
The animal blood cell analyzer according to claim 10, wherein the animal blood cell analyzer further comprises: a scanner;

The scanner is used to scan the information identifier on the test tube containing the blood of the animal to be tested to obtain sample information of the blood of the animal to be tested;

The mode selection module is used to select a target measurement mode from multiple animal measurement modes according to the sample information.
The animal blood cell analyzer according to claim 10, wherein the reagent delivery component is also used for:

Adding a target dose of diluent to the first counting cell component, and adding a hemolytic agent to the first counting cell component.
The animal blood cell analyzer according to claim 10, wherein:

The reagent delivery assembly is also used to add a first dose of the first counting pool to the first counting pool before or when the sampling needle assembly sends the blood of the animal to be tested into the first counting pool. Diluent;

The reagent delivery component is also used for adding a second dose of diluent and hemolytic agent to the first counting cell component after the sampling needle component adds the blood of the animal to be tested into the first counting cell component , The sum of the first dose and the second dose is the target dose.
The animal blood cell analyzer according to claim 14, wherein the animal blood cell analyzer further comprises: a second counting cell component;

The sampling needle assembly is also used to suck a fixed dose of a reference test sample composed of the first dose of diluent and the blood of the animal to be tested from the first counting cell assembly, and send it to the second Counting pool components;

The second counting cell component is used to perform red blood cell test and/or platelet test on the fixed dose of the reference test sample.
The animal blood cell analyzer according to claim 14 or 15, wherein the first dose is the same for different animal measurement modes.
The animal blood cell analyzer according to claim 14, wherein the reagent delivery assembly is further used to send the blood of the animal to be tested into the first counting cell assembly before the sampling needle assembly A first dose of diluent is added to a counting cell assembly.
The animal blood cell analyzer according to claim 14, wherein the reagent delivery component is also used for:

Before the sampling needle assembly sends the blood of the animal to be tested into the first counting pool assembly, adding a third dose of diluent to the first counting pool assembly;

When the sampling needle assembly sends the blood of the animal to be tested into the first counting cell assembly, a fourth dose of diluent is added to the first counting cell assembly, and the third dose and the fourth The sum of the doses is the first dose.
A storage medium having an executable program stored on the storage medium, and when the executable program is executed by a processor, the steps of the animal blood cell analysis method according to any one of claims 1 to 9 are realized.