WO2018168977A1 - Liquid composition for bacteriostasis or sterilization - Google Patents
Liquid composition for bacteriostasis or sterilization Download PDFInfo
- Publication number
- WO2018168977A1 WO2018168977A1 PCT/JP2018/010106 JP2018010106W WO2018168977A1 WO 2018168977 A1 WO2018168977 A1 WO 2018168977A1 JP 2018010106 W JP2018010106 W JP 2018010106W WO 2018168977 A1 WO2018168977 A1 WO 2018168977A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- liquid composition
- emulsifier
- composition
- phytic acid
- present
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 162
- 239000007788 liquid Substances 0.000 title claims abstract description 86
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 23
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 17
- 235000002949 phytic acid Nutrition 0.000 claims abstract description 57
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims abstract description 56
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims abstract description 56
- 239000000467 phytic acid Substances 0.000 claims abstract description 56
- 229940068041 phytic acid Drugs 0.000 claims abstract description 56
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 55
- 230000003385 bacteriostatic effect Effects 0.000 claims abstract description 52
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 53
- 230000000844 anti-bacterial effect Effects 0.000 claims description 47
- 238000000034 method Methods 0.000 claims description 19
- GHBFNMLVSPCDGN-UHFFFAOYSA-N rac-1-monooctanoylglycerol Chemical compound CCCCCCCC(=O)OCC(O)CO GHBFNMLVSPCDGN-UHFFFAOYSA-N 0.000 claims description 18
- 239000003125 aqueous solvent Substances 0.000 claims description 10
- LKUNXBRZDFMZOK-GFCCVEGCSA-N Capric acid monoglyceride Natural products CCCCCCCCCC(=O)OC[C@H](O)CO LKUNXBRZDFMZOK-GFCCVEGCSA-N 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 8
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 6
- 239000000194 fatty acid Substances 0.000 claims description 6
- 229930195729 fatty acid Natural products 0.000 claims description 6
- -1 glycerin fatty acid ester Chemical class 0.000 claims description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerol Natural products OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 4
- 235000011187 glycerol Nutrition 0.000 claims description 4
- 235000013305 food Nutrition 0.000 description 32
- 239000000243 solution Substances 0.000 description 17
- 241000894006 Bacteria Species 0.000 description 16
- 244000005700 microbiome Species 0.000 description 16
- 239000000463 material Substances 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 230000001580 bacterial effect Effects 0.000 description 11
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 239000002253 acid Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- 150000001298 alcohols Chemical class 0.000 description 7
- 238000002156 mixing Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000004310 lactic acid Substances 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 239000012046 mixed solvent Substances 0.000 description 5
- 239000013077 target material Substances 0.000 description 5
- 238000010411 cooking Methods 0.000 description 4
- 239000003002 pH adjusting agent Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 239000000645 desinfectant Substances 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical compound OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- AEQDJSLRWYMAQI-UHFFFAOYSA-N 2,3,9,10-tetramethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline Chemical compound C1CN2CC(C(=C(OC)C=C3)OC)=C3CC2C2=C1C=C(OC)C(OC)=C2 AEQDJSLRWYMAQI-UHFFFAOYSA-N 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 description 1
- 241000222290 Cladosporium Species 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 241001468192 Leuconostoc citreum Species 0.000 description 1
- 241000192130 Leuconostoc mesenteroides Species 0.000 description 1
- 241001468196 Leuconostoc pseudomesenteroides Species 0.000 description 1
- UEZVMMHDMIWARA-UHFFFAOYSA-N Metaphosphoric acid Chemical compound OP(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241001138501 Salmonella enterica Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 241000186882 Weissella viridescens Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000001361 adipic acid Substances 0.000 description 1
- 235000011037 adipic acid Nutrition 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-N diphosphoric acid Chemical compound OP(O)(=O)OP(O)(O)=O XPPKVPWEQAFLFU-UHFFFAOYSA-N 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000003084 food emulsifier Nutrition 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 235000011167 hydrochloric acid Nutrition 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000137 polyphosphoric acid Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 229940005657 pyrophosphoric acid Drugs 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 239000000176 sodium gluconate Substances 0.000 description 1
- 229940005574 sodium gluconate Drugs 0.000 description 1
- 235000012207 sodium gluconate Nutrition 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B2/00—Preservation of foods or foodstuffs, in general
- A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
- A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
- A23B2/729—Organic compounds; Microorganisms; Enzymes
- A23B2/742—Organic compounds containing oxygen
- A23B2/754—Organic compounds containing oxygen containing carboxyl groups
- A23B2/758—Carboxylic acid esters
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B2/00—Preservation of foods or foodstuffs, in general
- A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
- A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
- A23B2/729—Organic compounds; Microorganisms; Enzymes
- A23B2/775—Organic compounds containing phosphorus
Definitions
- the present invention relates to a bactericidal or bacteriostatic composition.
- Patent Document 1 describes a food disinfectant composition containing a fatty acid monoester, an acid or chelating agent, a food grade surfactant and a medium.
- Patent Document 2 describes an emulsion stabilizer for a beverage containing milk containing phytic acid and an emulsifier.
- Patent Document 3 discloses an antibacterial substance derived from horseradish, an organic acid or a salt thereof, an amino acid, a lower fatty acid ester, a sugar ester, a vitamin B1 ester, a polymerized phosphate, phytic acid, ethanol, and the like.
- Patent Document 4 discloses a disinfectant composition containing ethanol, sodium gluconate, capric acid, caprylic acid monoglyceride and capric acid monoglyceride, a pH adjuster, water, and an acidity of an aqueous solution of 0.1 to 0.9. Are listed.
- compositions for sterilization or bacteriostasis of food or the equipment that handles it or the environment in which it exists not only the bactericidal or bacteriostatic effect is important, but also the safety cannot be ignored.
- compositions that have been used in the field of food to control the growth of microorganisms meet the safety standards, but are difficult to form a homogeneous liquid with low water solubility. Often difficult to use. Therefore, a composition that is homogeneous liquid, has high sterilization or bacteriostatic effect and is highly safe for living organisms, and is useful for sterilization or bacteriostasis of food or the equipment in which it is present or the environment where it exists. It has been demanded.
- the present inventors have found that a liquid composition containing an emulsifier and phytic acid and having a pH of 3.5 or less is easy to handle and has a high bactericidal or bacteriostatic effect. Furthermore, the present inventors have found that the growth of microorganisms in the material can be suppressed for a long time by spraying or applying the liquid composition to the material.
- the present invention provides a bactericidal or bacteriostatic liquid composition comprising an emulsifier and phytic acid and having a pH of 3.5 or lower.
- the present invention also provides a liquid composition for imparting antibacterial properties to a subject, which contains an emulsifier and phytic acid and has a pH of 3.5 or lower.
- the present invention further includes spraying or applying the liquid composition to the object, or immersing the object in the liquid composition,
- the liquid composition contains an emulsifier and phytic acid and has a pH of 3.5 or less. Disinfecting or bacteriostatic methods are provided.
- the present invention further includes spraying or applying the liquid composition to the object, or immersing the object in the liquid composition,
- the liquid composition contains an emulsifier and phytic acid and has a pH of 3.5 or less.
- the present invention provides use of a liquid composition containing an emulsifier and phytic acid and having a pH of 3.5 or less for sterilization or bacteriostasis. Furthermore, the present invention provides use of a liquid composition containing an emulsifier and phytic acid and having a pH of 3.5 or less for imparting antibacterial properties to a subject.
- the present invention provides the use of a liquid composition comprising an emulsifier and phytic acid and having a pH of 3.5 or less for the production of a bactericidal or bacteriostatic liquid composition. Furthermore, the present invention provides use of a liquid composition containing an emulsifier and phytic acid and having a pH of 3.5 or less for producing a liquid composition for imparting antibacterial properties to a subject.
- the liquid composition of the present invention is liquid, it is easy to use, and has a high bactericidal or bacteriostatic effect and is highly safe for living bodies. Further, when the liquid composition of the present invention is applied to a material, it can sterilize or bacterioculate microorganisms attached to the material, and can continuously suppress the growth of microorganisms in the material for a long time. Therefore, the present invention is effective in the field where microbial growth suppression is constantly desired, for example, the field of food production, processing, storage and the like.
- the present invention provides a bactericidal or bacteriostatic composition.
- the bactericidal or bacteriostatic composition of the present invention contains an emulsifier and phytic acid.
- emulsifiers used in the present invention include emulsifiers that can be used in foods, for example, glycerin fatty acid esters such as caprylic acid monoglyceride and capric acid monoglyceride, preferably at least selected from caprylic acid monoglyceride and capric acid monoglyceride One type. These food emulsifiers are commercially available.
- caprylic acid monoglyceride is Sunsoft (registered trademark) 700p-2 (Taiyo Chemical Co., Ltd.), Poem M-100 (Riken Vitamin Co., Ltd.), and capric acid monoglyceride is Sunsoft (registered trademark) 760 (Taiyo Chemical). Co., Ltd.) and Poem M-200 (RIKEN Vitamin Co., Ltd.).
- the concentration of the emulsifier in the composition of the present invention may vary depending on the type thereof, but may be 0.005% by mass or more, preferably 0.01% by mass or more, more preferably 0% in the total amount of the composition. 0.05% by mass or more, more preferably 0.1% by mass or more, and still more preferably 0.15% by mass or more.
- concentration of the emulsifier in the composition of the present invention is preferably 10.0% by mass or less, more preferably 5.0% by mass or less, and further preferably 2.0% by mass or less, from the viewpoints of solubility and economy. In addition, Preferably it is 1.0 mass% or less.
- Physic acid is conventionally applied to foods and the like as a binder, acidulant and the like.
- Phytic acid is commercially available, for example, sold under a trade name such as a 50% aqueous solution of phytic acid (IP6) (Tsukino Foods Co., Ltd.).
- IP6 50% aqueous solution of phytic acid
- the concentration of phytic acid in the composition of the present invention may vary depending on the concentration of the emulsifier, but in the total amount of the composition, it may be 0.005% by mass or more, preferably 0.01% by mass or more, more preferably It is 0.05 mass% or more, More preferably, it is 0.1 mass% or more. When the concentration of phytic acid is low, the composition of the present invention cannot obtain sufficient bactericidal or bacteriostatic properties.
- the concentration of phytic acid in the composition of the present invention is preferably 5% by mass or less. If the concentration of phytic acid is too high, the pH of the composition of the present invention will be low, which may adversely affect the application environment of the composition of the present invention, such as metal corrosion.
- the ratio of the emulsifier and phytic acid is, by mass ratio, preferably 1: 0.005 to 1: 200, preferably 1: 0.1 to 1: 200, more preferably 1: 0. .1 to 1:40, more preferably 1: 0.3 to 1:40. If the ratio is outside the above range, the composition of the present invention may become unacceptable in terms of quality and cost.
- the emulsifier and phytic acid contained in the sterilizing or bacteriostatic composition of the present invention are both substances that are acceptable as food additives. Therefore, the composition of the present invention has higher safety for the human body than general cleaning agents and disinfectants. Therefore, the composition of the present invention is useful for application in food-related fields, for example, sterilization or bacteriostasis of food, or the equipment in which it is handled or the environment in which it exists.
- the composition of the present invention comprises an emulsifier and other components other than phytic acid, such as phosphoric acid other than phytic acid (ultraphosphoric acid, polyphosphoric acid, pyrophosphoric acid, metaphosphoric acid), citric acid, gluconic acid, fumaric acid, maleic acid.
- phosphoric acid other than phytic acid ultraphosphoric acid, polyphosphoric acid, pyrophosphoric acid, metaphosphoric acid
- citric acid gluconic acid
- fumaric acid maleic acid
- At least one selected from the group consisting of organic acids and inorganic acids such as acids, malonic acid, tartaric acid, succinic acid, malic acid, adipic acid and hydrochloric acid, and salts thereof, a pH adjuster usable in foods, etc. You may contain.
- the types and contents of these other components are limited to the extent that the bactericidal or bacteriostatic properties necessary for the composition of the present invention are not lost.
- the composition of the present invention may further contain alcohols.
- alcohols used in the composition of the present invention include alcohols that can be used in foods such as ethanol. Addition of alcohols improves the solubility of the emulsifier and makes the composition of the present invention easy to handle.
- the content of alcohols in the composition of the present invention may be equal to or less than the balance of the above-described emulsifier, phytic acid and other components, preferably the balance of the emulsifier, phytic acid and other components, more preferably And 20 to 60% by mass in the total amount of the composition.
- the composition of the present invention may further contain an aqueous solvent.
- a preferable aqueous solvent includes, for example, water.
- the content of the aqueous solvent in the composition of the present invention may be equal to or less than the balance of the above-described emulsifier, phytic acid and other components, preferably the emulsifier, phytic acid, other components and the balance of alcohols. More preferably, it is 20% by mass or more.
- composition of the present invention is a liquid composition in which an emulsifier and phytic acid are dissolved in the aqueous solvent.
- a liquid composition in which an emulsifier and phytic acid are dissolved in the alcohol is another example of the composition of the present invention.
- a preferred example of the composition of the present invention is an aqueous ethanol solution in which an emulsifier and phytic acid are dissolved in a mixed solvent of ethanol / water.
- the ethanol / water mixed solvent is preferably a 20 to 60% by mass aqueous ethanol solution.
- the content of the mixed solvent of the alcohol and the aqueous solvent or the mixed solvent of the ethanol / water in the composition of the present invention may be the balance of the above-described emulsifier, phytic acid and other components.
- the pH of the composition of the present invention is preferably 3.5 or less, more preferably 3.0 or less. If the pH is high, the composition of the present invention may not obtain sufficient bactericidal or bacteriostatic properties. On the other hand, if the pH is too low, it may adversely affect the application environment of the composition of the present invention, such as metal corrosion. May cause effects.
- the pH of the composition of the present invention can be adjusted mainly by the addition of phytic acid, but an acid other than the above-described phytic acid or a pH adjuster that can be used in foods may be used. Preferably, the composition of the present invention does not contain any acid other than phytic acid or a pH adjuster.
- compositions of the present invention include a composition containing 0.01% by mass or more of an emulsifier, 0.005% by mass or more of phytic acid and having a pH of 3.0 or less.
- a preferable composition of the present invention includes a composition containing 0.15% or more of an emulsifier, 0.005% by mass or more of phytic acid, and having a pH of 3.5 or less.
- the composition according to the invention contains 20 to 60% by weight of ethanol.
- the composition of the present invention has an excellent bactericidal or bacteriostatic action, and can be used for bactericidal or bacteriostatic of various substances.
- sterilization refers to killing microorganisms (but not limited to complete killing)
- bacteriostasis refers to inhibiting the growth of microorganisms. Therefore, in the subject to which the sterilizing or bacteriostatic composition of the present invention is applied, the growth of microorganisms is suppressed or the number of microorganisms is reduced.
- the kind of microorganisms sterilized or bacteriostatic by the composition of the present invention is not particularly limited. Yeast, molds of the genus Cladosporium, Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa L. , Etc.
- acid-resistant bacteria such as lactic acid bacteria have conventionally been difficult to remove or suppress growth because they survive even in a low pH region where normal microorganisms are inhibited from growing.
- the composition of the present invention is also effective for microbial species that have been difficult to remove in the past, such as acid-resistant bacteria, the utility value is high.
- the composition of the present invention can be used for sterilization or bacteriostatic of various materials.
- the application target of the composition of the present invention is not particularly limited as long as contact with the composition of the present invention is allowed.
- Preferable examples of the application target of the composition of the present invention include foods, equipment for handling foods, and environments where foods are present.
- Equipment that handles the food includes equipment that may come into contact with food in the process of production, processing, distribution, storage, cooking, etc., for example, equipment on the production line of a food production or processing plant, storage or transportation of food Containers, cooking utensils, cooking tables, workers' gloves and apron, work clothes, etc.
- the environment in which food exists is the environment in which food is handled, for example, the environment in which food is manufactured, processed, distributed, stored, cooked, etc., and more specifically, the food manufacturing or processing factory, food storage, cooking place, etc. Walls, floors, ceilings, pillars, doors, windows, ventilation holes.
- the sterilization or bacteriostasis of an object by the sterilization or bacteriostatic composition of the present invention can be performed by bringing the composition of the present invention into contact with the object.
- the composition for bactericidal or bacteriostatic of the present invention is liquid, it can be easily applied to a subject to be bactericidal or bacteriostatic.
- the liquid composition of the present invention may be mixed in a substance to be sterilized or bacteriostatic (for example, food).
- the liquid composition of the present invention may be sprayed or applied to a substance to be sterilized or bacteriostatic, or the target substance may be immersed in the liquid composition of the present invention.
- the liquid composition of the present invention is sprayed or applied to a subject to be sterilized or bacteriostatic.
- Objects that come into contact with the liquid composition of the present invention are sterilized or bacteriostatic.
- the composition of the present invention is brought into contact with the subject by spraying, applying, or immersing the composition of the present invention on the subject to be sterilized or bacteriostatic.
- the object brought into contact with the composition of the present invention may be dried.
- the drying method include, but are not particularly limited to, drying by standing, air drying, and drying by a dryer.
- volatile components contained in the composition of the present invention such as an aqueous solvent and ethanol, are volatilized and removed from the target. Even after the removal of the volatile components, the bactericidal or bacteriostatic action continues in the subject to which the composition of the present invention is applied. Therefore, in this embodiment, the composition of the present invention not only exhibits sterilization or bacteriostatic activity against a subject in a liquid state, but also suppresses the growth of microorganisms in the subject even in a dry matter state.
- composition of the present invention may be a liquid composition for imparting antibacterial properties to a subject.
- the antibacterial property is imparted to the subject by adhering the composition of the present invention to the subject to be imparted with the antibacterial property by spraying, coating, dipping, or the like. If necessary or preferably, the object to which the composition of the present invention is further adhered is dried.
- antibacterial of a subject refers to the property of the subject that microorganisms attached to or invaded the surface of the subject can be sterilized or bacteriostatic. This aspect is very useful for an environment where it is desired to constantly prevent microbial growth, or for materials and equipment used therein, for example, an environment where food is handled or food handling equipment used there.
- the composition of the present invention is used at a dose of about 0.1 to 5.0 mL per 100 cm 2 area of the subject, whether used for sterilization or bacteriostasis of the subject, or for imparting antibacterial properties to the subject. Preferably, it is used at a dose of about 0.5 to 1.0 mL per 100 cm 2 area of the subject. Preferably, this dose of the composition is evenly applied to the subject, preferably by spraying or application.
- Test example 1 (Preparation of liquid composition) Prepare a solution by mixing emulsifier (caprylic acid monoglyceride or capric acid monoglyceride), ethanol and sterilized purified water at a ratio of 1: 2: 2 at room temperature, and sterilize it with various organic acids and the remaining ethanol. Finished purified water was added to prepare a liquid composition (solution) shown in Table 1. The pH of the obtained liquid composition was measured. In addition, the bactericidal or bacteriostatic properties of each liquid composition were evaluated by the following procedure.
- emulsifier caprylic acid monoglyceride or capric acid monoglyceride
- Bactericidal or bacteriostatic characteristics evaluation Based on the number of bacteria measured in (2), the bactericidal or bacteriostatic characteristics of each composition (liquid group and dry group) were evaluated according to the following evaluation criteria.
- the number of bacteria decreased to 1/10 or less of the first time and more than 1/100 of the first time.
- D The number of bacteria is more than 1/10 of the first
- Test example 2 In the same procedure as in Test Example 1, except that the concentration of phytic acid was changed, liquid compositions (solutions) shown in Tables 2 to 3 were prepared and evaluated for bactericidal or bacteriostatic properties.
- Test example 3 In the same procedure as in Test Example 1, but changing the concentration of the emulsifier, liquid compositions (solutions) shown in Tables 4 to 5 were prepared and evaluated for bactericidal or bacteriostatic properties.
- Test example 4 The sterilization or bacteriostatic action of the liquid composition on various materials was investigated.
- a liquid composition caprylic acid monoglyceride 0.15%, phytic acid 0.40%, ethanol 40.00%, water residue; unit is mass%; pH 2.00
- sample bacteria A liquid was prepared.
- target materials metal (stainless steel plate), rubber (rubber gloves), and plastic (guard cover) were prepared.
- the stainless steel plate and watch cover were cut into 10 cm squares.
- the rubber gloves were stretched on a 10 cm square plastic plate so as not to be wrinkled and attached with double-sided tape, and the remaining part was cut and used.
- the number of bacteria was measured according to the following procedure for each material.
- Each target material was divided into 3 groups.
- 0.5 mL of the liquid composition was evenly applied onto the target material with a large rod, then 0.1 mL of the sample bacterial solution was inoculated, and spread with mixing with a large rod for 40 seconds (0 h group).
- 0.5 mL or 1.0 mL of the liquid composition was evenly applied onto the target material with a large stick and then air-dried in a safety cabinet for 18 hours to completely evaporate moisture and ethanol. .
- the initial bacterial count of the sample was adjusted to 10 5 to 10 6 cfu / mL.
- the target material to which the sample bacterial solution was applied was allowed to stand for 5 minutes, then placed in a sterile pouch, added with 10 mL of sterilized water from above, heat-sealed, and gently shaken up and down about 5 times.
- the pouch was opened, the liquid inside was collected, the number of bacteria was measured with MRS agar medium, and bactericidal or bacteriostatic properties were evaluated according to the same criteria as in Test Example 1.
- composition of the present invention is effective for sterilization or bacteriostatic of various materials both immediately after application (0 h group) and when the time after application has elapsed (18 h group).
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Abstract
Description
本発明は、殺菌又は静菌用組成物に関する。 The present invention relates to a bactericidal or bacteriostatic composition.
従来、食品製造においては、微生物の増殖による食品の腐敗や変質を防ぐための組成物が使用されている。特許文献１には、脂肪酸モノエステル、酸またはキレート化剤、食品グレードの界面活性剤及び媒体を含有する食品用殺菌剤組成物が記載されている。特許文献２には、フィチン酸及び乳化剤を含有する乳入り飲料用乳化安定剤が記載されている。特許文献３には、ホースラディッシュ由来の抗菌性物質と、有機酸又はその塩、アミノ酸、低級脂肪酸エステル、シュガーエステル、ビタミンＢ１エステル、重合リン酸塩、フィチン酸、エタノール等から選択される１種又は２種以上とを含有する食品用保存剤が記載されている。特許文献４には、エタノール、グルコン酸ナトリウム、カプリン酸、カプリル酸モノグリセリド及びカプリン酸モノグリセリド、ｐＨ調整剤、水を含有し、水溶液の酸度が０．１～０．９である殺菌剤組成物が記載されている。 Conventionally, in food production, compositions for preventing food spoilage and alteration due to the growth of microorganisms have been used. Patent Document 1 describes a food disinfectant composition containing a fatty acid monoester, an acid or chelating agent, a food grade surfactant and a medium. Patent Document 2 describes an emulsion stabilizer for a beverage containing milk containing phytic acid and an emulsifier. Patent Document 3 discloses an antibacterial substance derived from horseradish, an organic acid or a salt thereof, an amino acid, a lower fatty acid ester, a sugar ester, a vitamin B1 ester, a polymerized phosphate, phytic acid, ethanol, and the like. Or the preservative for foods containing 2 or more types is described. Patent Document 4 discloses a disinfectant composition containing ethanol, sodium gluconate, capric acid, caprylic acid monoglyceride and capric acid monoglyceride, a pH adjuster, water, and an acidity of an aqueous solution of 0.1 to 0.9. Are listed.
食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌のための組成物においては、その殺菌又は静菌効果が重要であるだけでなく、安全性も無視できない。一方で、食品分野で微生物の増殖制御に従来使用されている組成物は、安全性の基準は満たすが、水溶性が低く均質な液状とすることが難しいため、機器等の洗浄や殺菌には使いづらいことが多い。したがって、均質な液状を呈し、高い殺菌又は静菌効果を有しながらも生体に対する安全性は高く、食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌に有用な組成物が求められている。 In the composition for sterilization or bacteriostasis of food or the equipment that handles it or the environment in which it exists, not only the bactericidal or bacteriostatic effect is important, but also the safety cannot be ignored. On the other hand, compositions that have been used in the field of food to control the growth of microorganisms meet the safety standards, but are difficult to form a homogeneous liquid with low water solubility. Often difficult to use. Therefore, a composition that is homogeneous liquid, has high sterilization or bacteriostatic effect and is highly safe for living organisms, and is useful for sterilization or bacteriostasis of food or the equipment in which it is present or the environment where it exists. It has been demanded.
さらに、食品を取り扱う機器においては、殺菌処理直後だけでなく、機器の使用中にわたって減菌された状態を維持することが望まれる。したがって、素材についた微生物を殺菌又は静菌した後、そのまま継続的に該素材における微生物の増殖を抑えることを可能にする組成物の開発は、非常に有用であると考えられる。 Furthermore, it is desirable for devices that handle food to maintain a sterilized state not only immediately after sterilization but also during use of the device. Therefore, it is considered that the development of a composition capable of continuously suppressing the growth of microorganisms in the material after sterilizing or bacteriostatizing the microorganism attached to the material is very useful.
本発明者らは、乳化剤とフィチン酸を含み、かつｐＨが３．５以下である液状組成物が、扱いやすく、また高い殺菌又は静菌作用を有することを見出した。さらに本発明者らは、当該液状組成物を素材に噴霧又は塗布することで、該素材における微生物の増殖を長時間にわたって抑制できることを見出した。 The present inventors have found that a liquid composition containing an emulsifier and phytic acid and having a pH of 3.5 or less is easy to handle and has a high bactericidal or bacteriostatic effect. Furthermore, the present inventors have found that the growth of microorganisms in the material can be suppressed for a long time by spraying or applying the liquid composition to the material.
したがって、本発明は、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である、殺菌又は静菌用液状組成物を提供する。
また本発明は、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である、対象への抗菌性付与用液状組成物を提供する。
Therefore, the present invention provides a bactericidal or bacteriostatic liquid composition comprising an emulsifier and phytic acid and having a pH of 3.5 or lower.
The present invention also provides a liquid composition for imparting antibacterial properties to a subject, which contains an emulsifier and phytic acid and has a pH of 3.5 or lower.
さらに本発明は、液状組成物を対象に噴霧もしくは塗布するか、又は該液状組成物に対象を浸漬させることを含み、
該液状組成物が、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である、
殺菌又は静菌方法を提供する。
さらに本発明は、液状組成物を対象に噴霧もしくは塗布するか、又は該液状組成物に対象を浸漬させることを含み、
該液状組成物が、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である、
対象への抗菌性付与方法を提供する。
The present invention further includes spraying or applying the liquid composition to the object, or immersing the object in the liquid composition,
The liquid composition contains an emulsifier and phytic acid and has a pH of 3.5 or less.
Disinfecting or bacteriostatic methods are provided.
The present invention further includes spraying or applying the liquid composition to the object, or immersing the object in the liquid composition,
The liquid composition contains an emulsifier and phytic acid and has a pH of 3.5 or less.
Provide a method for imparting antibacterial properties to a subject.
さらに本発明は、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である液状組成物の、殺菌又は静菌のための使用を提供する。
さらに本発明は、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である液状組成物の、対象への抗菌性付与のための使用を提供する。
Furthermore, the present invention provides use of a liquid composition containing an emulsifier and phytic acid and having a pH of 3.5 or less for sterilization or bacteriostasis.
Furthermore, the present invention provides use of a liquid composition containing an emulsifier and phytic acid and having a pH of 3.5 or less for imparting antibacterial properties to a subject.
さらに本発明は、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である液状組成物の、殺菌又は静菌用液状組成物の製造のための使用を提供する。
さらに本発明は、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である液状組成物の、対象への抗菌性付与用液状組成物の製造のための使用を提供する。
Furthermore, the present invention provides the use of a liquid composition comprising an emulsifier and phytic acid and having a pH of 3.5 or less for the production of a bactericidal or bacteriostatic liquid composition.
Furthermore, the present invention provides use of a liquid composition containing an emulsifier and phytic acid and having a pH of 3.5 or less for producing a liquid composition for imparting antibacterial properties to a subject.
本発明の液状組成物は、液状であるため使用しやすく、また高い殺菌又は静菌効果を有しながらも生体に対する安全性が高い。また、本発明の液状組成物は、素材に適用すれば、該素材についた微生物を殺菌又は静菌することができ、さらに長時間にわたって該素材における微生物の増殖を継続的に抑えることができる。したがって、本発明は、恒常的に微生物増殖抑制が望まれる分野、例えば、食品の製造、加工、保存等の分野において有効である。 Since the liquid composition of the present invention is liquid, it is easy to use, and has a high bactericidal or bacteriostatic effect and is highly safe for living bodies. Further, when the liquid composition of the present invention is applied to a material, it can sterilize or bacterioculate microorganisms attached to the material, and can continuously suppress the growth of microorganisms in the material for a long time. Therefore, the present invention is effective in the field where microbial growth suppression is constantly desired, for example, the field of food production, processing, storage and the like.
本発明は、殺菌又は静菌用組成物を提供する。本発明の殺菌又は静菌用組成物は、乳化剤とフィチン酸とを含有する。 The present invention provides a bactericidal or bacteriostatic composition. The bactericidal or bacteriostatic composition of the present invention contains an emulsifier and phytic acid.
本発明で使用される乳化剤としては、食品に使用可能な乳化剤、例えば、カプリル酸モノグリセリド、カプリン酸モノグリセリド等のグリセリン脂肪酸エステルが挙げられ、好ましくは、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種である。これら食品用の乳化剤は、市販されている。例えば、カプリル酸モノグリセリドは、サンソフト（登録商標）７００ｐ－２（太陽化学株式会社）、ポエムＭ－１００（理研ビタミン株式会社）など、カプリン酸モノグリセリドは、サンソフト（登録商標）７６０（太陽化学株式会社）、ポエムＭ－２００（理研ビタミン株式会社）などの商品名で販売されている。 Examples of the emulsifier used in the present invention include emulsifiers that can be used in foods, for example, glycerin fatty acid esters such as caprylic acid monoglyceride and capric acid monoglyceride, preferably at least selected from caprylic acid monoglyceride and capric acid monoglyceride One type. These food emulsifiers are commercially available. For example, caprylic acid monoglyceride is Sunsoft (registered trademark) 700p-2 (Taiyo Chemical Co., Ltd.), Poem M-100 (Riken Vitamin Co., Ltd.), and capric acid monoglyceride is Sunsoft (registered trademark) 760 (Taiyo Chemical). Co., Ltd.) and Poem M-200 (RIKEN Vitamin Co., Ltd.).
本発明の組成物における乳化剤の濃度は、その種類によって変動はあり得るが、該組成物全量中、０．００５質量％以上であるとよく、好ましくは０．０１質量％以上、より好ましくは０．０５質量％以上、さらに好ましくは０．１質量％以上、なお好ましくは０．１５質量％以上である。乳化剤の濃度が低いと、本発明の組成物は充分な殺菌又は静菌特性を得られない。一方、本発明の組成物における乳化剤の濃度は、溶解性及び経済性の観点から、好ましくは１０．０質量％以下、より好ましくは５．０質量％以下、さらに好ましくは２．０質量％以下、なお好ましくは１．０質量％以下である。 The concentration of the emulsifier in the composition of the present invention may vary depending on the type thereof, but may be 0.005% by mass or more, preferably 0.01% by mass or more, more preferably 0% in the total amount of the composition. 0.05% by mass or more, more preferably 0.1% by mass or more, and still more preferably 0.15% by mass or more. When the concentration of the emulsifier is low, the composition of the present invention cannot obtain sufficient bactericidal or bacteriostatic properties. On the other hand, the concentration of the emulsifier in the composition of the present invention is preferably 10.0% by mass or less, more preferably 5.0% by mass or less, and further preferably 2.0% by mass or less, from the viewpoints of solubility and economy. In addition, Preferably it is 1.0 mass% or less.
フィチン酸は、従来、結着剤、酸味料等として食品等に適用されている。フィチン酸は、市販されており、例えばフィチン酸５０％水溶液（ＩＰ６）（築野食品株式会社）などの商品名で販売されている。本発明の組成物におけるフィチン酸の濃度は、乳化剤の濃度によっても異なり得るが、該組成物全量中、０．００５質量％以上であるとよく、好ましくは０．０１質量％以上、より好ましくは０．０５質量％以上、さらに好ましくは０．１質量％以上である。フィチン酸の濃度が低いと、本発明の組成物が充分な殺菌又は静菌特性を得られない。一方、本発明の組成物におけるフィチン酸の濃度は、好ましくは５質量％以下である。フィチン酸の濃度が高すぎると、本発明の組成物のｐＨが低くなり、金属の腐食など、本発明の組成物の適用環境に悪影響を及ぼすおそれがある。 Physic acid is conventionally applied to foods and the like as a binder, acidulant and the like. Phytic acid is commercially available, for example, sold under a trade name such as a 50% aqueous solution of phytic acid (IP6) (Tsukino Foods Co., Ltd.). The concentration of phytic acid in the composition of the present invention may vary depending on the concentration of the emulsifier, but in the total amount of the composition, it may be 0.005% by mass or more, preferably 0.01% by mass or more, more preferably It is 0.05 mass% or more, More preferably, it is 0.1 mass% or more. When the concentration of phytic acid is low, the composition of the present invention cannot obtain sufficient bactericidal or bacteriostatic properties. On the other hand, the concentration of phytic acid in the composition of the present invention is preferably 5% by mass or less. If the concentration of phytic acid is too high, the pH of the composition of the present invention will be low, which may adversely affect the application environment of the composition of the present invention, such as metal corrosion.
本発明の組成物において、乳化剤とフィチン酸との比率は、質量比で、好ましくは１：０．００５～１：２００、好ましくは１：０．１～１：２００、より好ましくは１：０．１～１：４０、さらに好ましくは１：０．３～１：４０である。上記比率を外れると、本発明の組成物が、品質、コスト面で許容できないものになることがある。 In the composition of the present invention, the ratio of the emulsifier and phytic acid is, by mass ratio, preferably 1: 0.005 to 1: 200, preferably 1: 0.1 to 1: 200, more preferably 1: 0. .1 to 1:40, more preferably 1: 0.3 to 1:40. If the ratio is outside the above range, the composition of the present invention may become unacceptable in terms of quality and cost.
本発明の殺菌又は静菌用組成物に含まれる乳化剤及びフィチン酸は、いずれも食品添加物として許容されている物質である。したがって、本発明の組成物は、一般的な洗浄剤や殺菌剤と比べて人体に対する安全性が高い。したがって、本発明の組成物は、食品関連分野への適用、例えば、食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌に有用である。 The emulsifier and phytic acid contained in the sterilizing or bacteriostatic composition of the present invention are both substances that are acceptable as food additives. Therefore, the composition of the present invention has higher safety for the human body than general cleaning agents and disinfectants. Therefore, the composition of the present invention is useful for application in food-related fields, for example, sterilization or bacteriostasis of food, or the equipment in which it is handled or the environment in which it exists.
本発明の組成物は、乳化剤及びフィチン酸以外の他の成分、例えば、フィチン酸以外のリン酸（ウルトラリン酸、ポリリン酸、ピロリン酸、メタリン酸）、クエン酸、グルコン酸、フマル酸、マレイン酸、マロン酸、酒石酸、コハク酸、リンゴ酸、アジピン酸、塩酸等の有機酸及び無機酸、ならびにそれらの塩からなる群より選択される少なくとも１種、食品に使用可能なｐＨ調整剤などを含有していてもよい。ただし、これらの他の成分の種類及び含有量は、本発明の組成物に必要な殺菌又は静菌特性が失われない範囲に制限される。好ましくは、本発明の組成物における当該他の成分の含有量は、５質量％以下である。 The composition of the present invention comprises an emulsifier and other components other than phytic acid, such as phosphoric acid other than phytic acid (ultraphosphoric acid, polyphosphoric acid, pyrophosphoric acid, metaphosphoric acid), citric acid, gluconic acid, fumaric acid, maleic acid. At least one selected from the group consisting of organic acids and inorganic acids such as acids, malonic acid, tartaric acid, succinic acid, malic acid, adipic acid and hydrochloric acid, and salts thereof, a pH adjuster usable in foods, etc. You may contain. However, the types and contents of these other components are limited to the extent that the bactericidal or bacteriostatic properties necessary for the composition of the present invention are not lost. Preferably, the content of the other component in the composition of the present invention is 5% by mass or less.
本発明の組成物は、さらにアルコール類を含有していてもよい。本発明の組成物に使用されるアルコール類としては、例えばエタノールなどの食品に使用可能なアルコール類が挙げられる。アルコール類の添加により、乳化剤の溶解性が向上し、本発明の組成物が扱いやすくなる。本発明の組成物におけるアルコール類の含有量は、上述した乳化剤、フィチン酸及び他の成分の残部以下であればよく、好ましくは該乳化剤、フィチン酸及び他の成分の残部であり、より好ましくは、該組成物全量中２０～６０質量％である。 The composition of the present invention may further contain alcohols. Examples of alcohols used in the composition of the present invention include alcohols that can be used in foods such as ethanol. Addition of alcohols improves the solubility of the emulsifier and makes the composition of the present invention easy to handle. The content of alcohols in the composition of the present invention may be equal to or less than the balance of the above-described emulsifier, phytic acid and other components, preferably the balance of the emulsifier, phytic acid and other components, more preferably And 20 to 60% by mass in the total amount of the composition.
あるいは、本発明の組成物は、さらに水性溶媒を含有していてもよい。好ましい水性溶媒としては、例えば水が挙げられる。本発明の組成物における該水性溶媒の含有量は、上述した乳化剤、フィチン酸及び他の成分の残部以下であればよく、好ましくは該乳化剤、フィチン酸、他の成分及びアルコール類の残部であり、さらに好ましくは２０質量％以上である。 Alternatively, the composition of the present invention may further contain an aqueous solvent. A preferable aqueous solvent includes, for example, water. The content of the aqueous solvent in the composition of the present invention may be equal to or less than the balance of the above-described emulsifier, phytic acid and other components, preferably the emulsifier, phytic acid, other components and the balance of alcohols. More preferably, it is 20% by mass or more.
本発明の組成物の一例は、上記水性溶媒に、乳化剤及びフィチン酸が溶解した液状組成物である。本発明の組成物の別の一例は、上記アルコール類に、乳化剤及びフィチン酸が溶解した液状組成物である。本発明の組成物の別の一例は、上記アルコール類と水性溶媒との混合溶媒に、乳化剤及びフィチン酸が溶解した液状組成物である。本発明の組成物の好ましい一例は、エタノール／水の混合溶媒に、乳化剤及びフィチン酸が溶解したエタノール水溶液である。該エタノール／水の混合溶媒としては、２０～６０質量％エタノール水溶液が好ましい。該アルコール類と水性溶媒との混合溶媒、又は該エタノール／水の混合溶媒の本発明の組成物における含有量は、上述した乳化剤、フィチン酸及び他の成分の残部であればよい。 An example of the composition of the present invention is a liquid composition in which an emulsifier and phytic acid are dissolved in the aqueous solvent. Another example of the composition of the present invention is a liquid composition in which an emulsifier and phytic acid are dissolved in the alcohol. Another example of the composition of the present invention is a liquid composition in which an emulsifier and phytic acid are dissolved in a mixed solvent of the above alcohols and an aqueous solvent. A preferred example of the composition of the present invention is an aqueous ethanol solution in which an emulsifier and phytic acid are dissolved in a mixed solvent of ethanol / water. The ethanol / water mixed solvent is preferably a 20 to 60% by mass aqueous ethanol solution. The content of the mixed solvent of the alcohol and the aqueous solvent or the mixed solvent of the ethanol / water in the composition of the present invention may be the balance of the above-described emulsifier, phytic acid and other components.
本発明の組成物のｐＨは、好ましくは３．５以下、より好ましくは３．０以下である。ｐＨが高くなると、本発明の組成物が充分な殺菌又は静菌特性を得られないことがあり、他方、ｐＨが低くなりすぎると、金属の腐食など、本発明の組成物の適用環境に悪影響を及ぼすおそれがある。本発明の組成物のｐＨの調整は、主にフィチン酸の添加によって行うことができるが、上述したフィチン酸以外の他の酸や、食品に使用可能なｐＨ調整剤を用いてもよい。好ましくは、本発明の組成物は、フィチン酸以外の他の酸やｐＨ調整剤を含有しない。 The pH of the composition of the present invention is preferably 3.5 or less, more preferably 3.0 or less. If the pH is high, the composition of the present invention may not obtain sufficient bactericidal or bacteriostatic properties. On the other hand, if the pH is too low, it may adversely affect the application environment of the composition of the present invention, such as metal corrosion. May cause effects. The pH of the composition of the present invention can be adjusted mainly by the addition of phytic acid, but an acid other than the above-described phytic acid or a pH adjuster that can be used in foods may be used. Preferably, the composition of the present invention does not contain any acid other than phytic acid or a pH adjuster.
好ましい本発明の組成物の例としては、乳化剤０．０１質量％以上、フィチン酸０．００５質量％以上を含有し、かつｐＨ３．０以下である組成物が挙げられる。好ましい本発明の組成物の別の例としては、乳化剤０．１５％以上、フィチン酸０．００５質量％以上を含有し、かつｐＨ３．５以下である組成物が挙げられる。好ましくは、本発明の組成物は２０～６０質量％のエタノールを含有する。 Preferred examples of the composition of the present invention include a composition containing 0.01% by mass or more of an emulsifier, 0.005% by mass or more of phytic acid and having a pH of 3.0 or less. Another example of a preferable composition of the present invention includes a composition containing 0.15% or more of an emulsifier, 0.005% by mass or more of phytic acid, and having a pH of 3.5 or less. Preferably, the composition according to the invention contains 20 to 60% by weight of ethanol.
本発明の組成物は、優れた殺菌又は静菌作用を有しており、各種物質の殺菌又は静菌に使用することができる。本明細書において、殺菌とは、微生物を死滅させること（但し完全な死滅に限られない）をいい、また静菌とは、微生物の増殖を抑制することをいう。したがって、本発明の殺菌又は静菌用組成物を適用された対象においては、微生物の増殖が抑制されるか、又は微生物の数が減少する。 The composition of the present invention has an excellent bactericidal or bacteriostatic action, and can be used for bactericidal or bacteriostatic of various substances. In this specification, sterilization refers to killing microorganisms (but not limited to complete killing), and bacteriostasis refers to inhibiting the growth of microorganisms. Therefore, in the subject to which the sterilizing or bacteriostatic composition of the present invention is applied, the growth of microorganisms is suppressed or the number of microorganisms is reduced.
本発明の組成物により殺菌又は静菌される微生物の種類としては、特に限定されないが、例えば、Ｗｅｉｓｓｅｌｌａ属、Ｌｅｕｃｏｎｏｓｔｏｃ属等の乳酸菌、Ｋｌｅｂｓｉｅｌｌａ属、Ｅｎｔｅｒｏｂａｃｔｅｒ属等の大腸菌群、Ｃａｎｄｉｄａ属、Ｃｒｙｐｔｏｃｏｃｃｕｓ属等の酵母、Ｃｌａｄｏｓｐｏｒｉｕｍ属等のカビ、大腸菌（Ｅｓｃｈｅｒｉｃｈｉａ ｃｏｌｉ）、サルモネラ菌（Ｓａｌｍｏｎｅｌｌａ ｅｎｔｅｒｉｃａ）、黄色ブドウ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ）、緑膿菌（Ｐｓｅｕｄｏｍｏｎａｓ ａｅｒｕｇｉｎｏｓａ）、リステリア菌（Ｌｉｓｔｅｒｉａ ｍｏｎｏｃｙｔｏｇｅｎｅｓ）、腸炎ビブリオ（Ｖｉｂｒｉｏ ｐａｒａｈａｅｍｏｌｙｔｉｃｕｓ）、などが挙げられる。特に、乳酸菌等の耐酸性菌は、通常の微生物が生育を阻害される低ｐＨ領域でも生存するため従来除去又は増殖抑制しにくいものであったが、これらも本発明の組成物により殺菌又は静菌することができる。このように、本発明の組成物は、耐酸性菌等の従来除去しにくかった微生物種にも有効であるため、利用価値が高い。 The kind of microorganisms sterilized or bacteriostatic by the composition of the present invention is not particularly limited. Yeast, molds of the genus Cladosporium, Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa L. , Etc. In particular, acid-resistant bacteria such as lactic acid bacteria have conventionally been difficult to remove or suppress growth because they survive even in a low pH region where normal microorganisms are inhibited from growing. Can be fungus. Thus, since the composition of the present invention is also effective for microbial species that have been difficult to remove in the past, such as acid-resistant bacteria, the utility value is high.
本発明の組成物は、各種素材の殺菌又は静菌に使用することができる。本発明の組成物の適用対象は、本発明の組成物との接触が許容されるものである限り、特に限定されない。本発明の組成物の適用対象の好ましい例としては、食品、食品を取り扱う機器、及び食品が存在する環境が挙げられる。該食品を取り扱う機器としては、製造、加工、流通、保存、調理等の過程で食品と接触する可能性のある機器、例えば、食品の製造もしくは加工工場の製造ラインの機器、食品の保存もしくは運搬用の容器、調理器具、調理台、作業者の手袋やエプロン、作業着、などが挙げられる。食品が存在する環境としては、食品を取り扱う環境、例えば食品の製造、加工、流通、保存、調理などが行われる環境、より詳細には、食品の製造もしくは加工工場、食品保管庫、調理場等の壁、床、天井、柱、ドア、窓、換気孔などが挙げられる。 The composition of the present invention can be used for sterilization or bacteriostatic of various materials. The application target of the composition of the present invention is not particularly limited as long as contact with the composition of the present invention is allowed. Preferable examples of the application target of the composition of the present invention include foods, equipment for handling foods, and environments where foods are present. Equipment that handles the food includes equipment that may come into contact with food in the process of production, processing, distribution, storage, cooking, etc., for example, equipment on the production line of a food production or processing plant, storage or transportation of food Containers, cooking utensils, cooking tables, workers' gloves and apron, work clothes, etc. The environment in which food exists is the environment in which food is handled, for example, the environment in which food is manufactured, processed, distributed, stored, cooked, etc., and more specifically, the food manufacturing or processing factory, food storage, cooking place, etc. Walls, floors, ceilings, pillars, doors, windows, ventilation holes.
本発明の殺菌又は静菌用組成物による対象の殺菌又は静菌は、該本発明の組成物を該対象に接触させることによって行われ得る。本発明の殺菌又は静菌用組成物は、液状であるため、殺菌又は静菌すべき対象への適用が容易である。例えば、本発明の液状組成物は、殺菌又は静菌対象の物質（例えば食品など）中に混合されてもよい。また例えば、本発明の液状組成物を、殺菌又は静菌対象の物質に噴霧もしくは塗布するか、又は本発明の液状組成物に該対象の物質を浸漬させてもよい。好ましくは、本発明の液状組成物は、殺菌又は静菌すべき対象に対して噴霧又は塗布される。本発明の液状組成物と接触した対象は、殺菌又は静菌される。 The sterilization or bacteriostasis of an object by the sterilization or bacteriostatic composition of the present invention can be performed by bringing the composition of the present invention into contact with the object. Since the composition for bactericidal or bacteriostatic of the present invention is liquid, it can be easily applied to a subject to be bactericidal or bacteriostatic. For example, the liquid composition of the present invention may be mixed in a substance to be sterilized or bacteriostatic (for example, food). Further, for example, the liquid composition of the present invention may be sprayed or applied to a substance to be sterilized or bacteriostatic, or the target substance may be immersed in the liquid composition of the present invention. Preferably, the liquid composition of the present invention is sprayed or applied to a subject to be sterilized or bacteriostatic. Objects that come into contact with the liquid composition of the present invention are sterilized or bacteriostatic.
本発明の好ましい態様においては、殺菌又は静菌すべき対象に対して本発明の組成物を噴霧もしくは塗布するか、又は浸漬させることで、該本発明の組成物と該対象とを接触させる。さらに、該本発明の組成物と接触させた対象を乾燥させてもよい。乾燥の方法としては、静置による乾燥、風乾、乾燥機による乾燥などが挙げられるが、特に限定されない。該乾燥により、本発明の組成物に含まれる揮発性成分、例えば水性溶媒やエタノールは、揮発して該対象から除去される。該揮発性成分が除去された後でも、本発明の組成物を適用した対象においては、殺菌又は静菌作用が持続する。したがって、本態様において、本発明の組成物は、液体の状態で対象に対して殺菌又は静菌力を発揮するだけでなく、乾燥物状態でも、該対象における微生物の増殖を抑える。 In a preferred embodiment of the present invention, the composition of the present invention is brought into contact with the subject by spraying, applying, or immersing the composition of the present invention on the subject to be sterilized or bacteriostatic. Furthermore, the object brought into contact with the composition of the present invention may be dried. Examples of the drying method include, but are not particularly limited to, drying by standing, air drying, and drying by a dryer. By the drying, volatile components contained in the composition of the present invention, such as an aqueous solvent and ethanol, are volatilized and removed from the target. Even after the removal of the volatile components, the bactericidal or bacteriostatic action continues in the subject to which the composition of the present invention is applied. Therefore, in this embodiment, the composition of the present invention not only exhibits sterilization or bacteriostatic activity against a subject in a liquid state, but also suppresses the growth of microorganisms in the subject even in a dry matter state.
したがって、本発明の組成物の別の態様は、対象への抗菌性付与用液状組成物であり得る。本発明の組成物を、抗菌性を付与したい対象に噴霧、塗布、浸漬等により付着させることにより、該対象に抗菌性が付与される。必要に応じて又は好ましくは、さらに該本発明の組成物を付着させた対象を乾燥させる。本明細書において、対象の「抗菌性」とは、該対象の表面に付着又は内部に侵入した微生物を殺菌又は静菌することができるという、該対象の性質をいう。本態様は、恒常的に微生物増殖を防止したい環境、又はそこで使用される素材や機器、例えば、食品を取り扱う環境又はそこで使用される食品取り扱い用機器に対して、非常に有用である。 Therefore, another embodiment of the composition of the present invention may be a liquid composition for imparting antibacterial properties to a subject. The antibacterial property is imparted to the subject by adhering the composition of the present invention to the subject to be imparted with the antibacterial property by spraying, coating, dipping, or the like. If necessary or preferably, the object to which the composition of the present invention is further adhered is dried. In the present specification, “antibacterial” of a subject refers to the property of the subject that microorganisms attached to or invaded the surface of the subject can be sterilized or bacteriostatic. This aspect is very useful for an environment where it is desired to constantly prevent microbial growth, or for materials and equipment used therein, for example, an environment where food is handled or food handling equipment used there.
本発明の組成物は、対象の殺菌又は静菌に用いる場合、及び対象への抗菌性付与に用いる場合のいずれにおいても、対象の面積１００ｃｍ2あたり０．１～５．０ｍＬ程度の用量で使用されることが好ましく、対象の面積１００ｃｍ2あたり０．５～１．０ｍＬ程度の用量で使用されることがより好ましい。好ましくは、この用量の組成物を、対象に対して、好ましくは噴霧又は塗布により、均等に付着させる。 The composition of the present invention is used at a dose of about 0.1 to 5.0 mL per 100 cm 2 area of the subject, whether used for sterilization or bacteriostasis of the subject, or for imparting antibacterial properties to the subject. Preferably, it is used at a dose of about 0.5 to 1.0 mL per 100 cm 2 area of the subject. Preferably, this dose of the composition is evenly applied to the subject, preferably by spraying or application.
以下、実施例を挙げて、本発明をさらに詳細に説明するが、本発明はこれらの実施例にのみ限定されるものではない。 Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited only to these examples.
試験例１
〔液状組成物の調製〕
室温下で、乳化剤（カプリル酸モノグリセリド又はカプリン酸モノグリセリド）、エタノール及び滅菌済み精製水を１：２：２の割合で混合して溶液を調製し、これに各種有機酸、及び残余のエタノールと滅菌済み精製水を添加して、表１記載の液状組成物（溶液）を調製した。得られた液状組成物のｐＨを測定した。また、下記の手順で各液状組成物の殺菌又は静菌特性を評価した。
Test example 1
(Preparation of liquid composition)
Prepare a solution by mixing emulsifier (caprylic acid monoglyceride or capric acid monoglyceride), ethanol and sterilized purified water at a ratio of 1: 2: 2 at room temperature, and sterilize it with various organic acids and the remaining ethanol. Finished purified water was added to prepare a liquid composition (solution) shown in Table 1. The pH of the obtained liquid composition was measured. In addition, the bactericidal or bacteriostatic properties of each liquid composition were evaluated by the following procedure.
〔殺菌又は静菌特性評価〕
（１）サンプル菌液の調製
対象菌には、４種の乳酸菌Ｗｅｉｓｓｅｌｌａ ｖｉｒｉｄｅｓｃｅｎｓ、Ｌｅｕｃｏｎｏｓｔｏｃ ｍｅｓｅｎｔｅｒｏｉｄｅｓ、Ｌｅｕｃｏｎｏｓｔｏｃ ｃｉｔｒｅｕｍ、及びＬｅｕｃｏｎｏｓｔｏｃ ｐｓｅｕｄｏｍｅｓｅｎｔｅｒｏｉｄｅｓを用いた。対象菌のシングルコロニーを、白金耳を用いて数コロニー釣菌し、ＴＳＢ培地に植菌して３０℃で２０時間種培養したものを乳酸菌液（１０7ｃｆｕ／ｍＬ）とした。得られた乳酸菌液を、滅菌済み精製水１０ｍＬに０．１ｍＬ混合し、さらにこれを１０倍希釈した。４種の乳酸菌の１０倍希釈液を等量混合してサンプル菌液を調製した。
[Bactericidal or bacteriostatic characteristics evaluation]
(1) Preparation of Sample Bacterial Solution Four types of lactic acid bacteria Weissella viridescens, Leuconostoc mesenteroides, Leuconostoc citreum, and Leuconostoc pseudomesenteroides were used. A single colony of the target bacterium was picked using a platinum loop, inoculated into a TSB medium, and seeded and cultured at 30 ° C. for 20 hours to obtain a lactic acid bacteria solution (10 7 cfu / mL). The obtained lactic acid bacteria solution was mixed with 0.1 mL of sterilized purified water and further diluted 10 times. A sample bacterial solution was prepared by mixing equal amounts of a 10-fold diluted solution of four types of lactic acid bacteria.
（２）液状組成物の適用及び菌数測定
塗布直後及び１８時間後の液状組成物による静菌特性を調べた。
滅菌プラスチックシャーレを二群に分けた。一方の群では、各液状組成物を１．０ｍＬずつ空のシャーレに入れ、コンラージ棒で均一に塗布し、次いでこれにサンプル菌液を１．０ｍＬ接種し、２０秒間コンラージ棒で混合しながら塗り広げた（０ｈ群）。もう一方の群では、各液状組成物を１．０ｍＬずつ空のシャーレに入れ、コンラージ棒で均一に塗布した後、安全キャビネット内で１８時間風乾し、水分とエタノールを完全に蒸発させた。これにサンプル菌液を１．０ｍＬ接種し、２０秒間コンラージ棒で混合しながら塗り広げた（１８ｈ群）。初発菌数は１０5～１０6ｃｆｕ/ｍＬに調整した。
サンプル菌液を塗布したシャーレを、５分間静置後、シャーレ内の液体を採取し、ＭＲＳ寒天培地を用いて菌数を測定した。
(2) Application of liquid composition and measurement of bacterial count The bacteriostatic characteristics of the liquid composition immediately after application and 18 hours later were examined.
Sterile plastic dishes were divided into two groups. In one group, 1.0 mL of each liquid composition is placed in an empty petri dish, and uniformly applied with a large stick, then 1.0 mL of the sample bacterial solution is inoculated, and mixed for 20 seconds with mixing with a large stick. Spread (0h group). In the other group, 1.0 mL of each liquid composition was placed in an empty petri dish, and uniformly applied with a large stick, then air-dried in a safety cabinet for 18 hours to completely evaporate water and ethanol. This was inoculated with 1.0 mL of the sample bacterial solution and spread with mixing with a large rod for 20 seconds (18h group). The initial bacterial count was adjusted to 10 5 to 10 6 cfu / mL.
The petri dish to which the sample bacterial solution was applied was allowed to stand for 5 minutes, and then the liquid in the petri dish was collected and the number of bacteria was measured using an MRS agar medium.
（３）殺菌又は静菌特性評価
（２）で測定した菌数をもとに、下記評価基準にて各組成物（液体群及び乾燥群）の殺菌又は静菌特性を評価した。
Ａ：初発の１／１０００以下に菌数が減少
Ｂ：初発の１／１００以下～１／１０００超に菌数が減少
Ｃ：初発の１／１０以下～１／１００超に菌数が減少
Ｄ：菌数が初発の１／１０よりも多い
(3) Bactericidal or bacteriostatic characteristics evaluation Based on the number of bacteria measured in (2), the bactericidal or bacteriostatic characteristics of each composition (liquid group and dry group) were evaluated according to the following evaluation criteria.
A: The number of bacteria decreased to 1/1000 or less of the first time. B: The number of bacteria decreased to 1/100 or less of the first time to more than 1/1000 C. The number of bacteria decreased to 1/10 or less of the first time and more than 1/100 of the first time. D : The number of bacteria is more than 1/10 of the first
結果を表１に示す。フィチン酸を含む液状組成物は、塗布直後（０ｈ群）及び塗布後時間が経過した場合（１８ｈ群）のいずれでも高い殺菌又は静菌作用を示したが、フィチン酸以外の酸を含む組成物は、塗布後時間が経過すると、殺菌又は静菌作用を示さなかった。 The results are shown in Table 1. The liquid composition containing phytic acid showed a high bactericidal or bacteriostatic action both immediately after application (0 h group) and when the time after application (18 h group) passed, but a composition containing an acid other than phytic acid Did not show bactericidal or bacteriostatic action over time after application.
試験例２
試験例１と同様の手順で、但しフィチン酸の濃度を変えて、表２～３の液状組成物（溶液）を調製し、殺菌又は静菌特性を評価した。
Test example 2
In the same procedure as in Test Example 1, except that the concentration of phytic acid was changed, liquid compositions (solutions) shown in Tables 2 to 3 were prepared and evaluated for bactericidal or bacteriostatic properties.
結果を表２～３に示す。フィチン酸の濃度が低くｐＨが３．５より高い組成物は、塗布後時間が経過すると、殺菌又は静菌作用を示さなかった。 The results are shown in Tables 2-3. A composition having a low concentration of phytic acid and a pH higher than 3.5 did not exhibit bactericidal or bacteriostatic action after a lapse of time after application.
試験例３
試験例１と同様の手順で、但し乳化剤の濃度を変えて、表４～５の液状組成物（溶液）を調製し、殺菌又は静菌特性を評価した。
Test example 3
In the same procedure as in Test Example 1, but changing the concentration of the emulsifier, liquid compositions (solutions) shown in Tables 4 to 5 were prepared and evaluated for bactericidal or bacteriostatic properties.
結果を表４～５に示す。いずれの組成物も、塗布直後（０ｈ群）だけでなく、塗布後時間が経過した場合（１８ｈ群）も殺菌又は静菌作用を維持していた。 The results are shown in Tables 4-5. All the compositions maintained the bactericidal or bacteriostatic action not only immediately after application (0 h group) but also after the application time (18 h group).
試験例４
様々な素材上における液状組成物の殺菌又は静菌作用を調べた。
試験例１と同様の手順で、液状組成物（カプリル酸モノグリセリド ０．１５％、フィチン酸 ０．４０％、エタノール ４０．００％、水 残余；単位は質量％；ｐＨ２．００）、及びサンプル菌液を調製した。
対象素材として、金属（ステンレス板）、ゴム（ゴム手袋）、プラスチック（番重ふた）を準備した。ステンレス板及び番重ふたは、１０ｃｍ四方に切断して使用した。ゴム手袋は、１０ｃｍ四方のプラスチック板に皺のないように伸ばして両面テープで貼り、余った部分はカットして使用した。
Test example 4
The sterilization or bacteriostatic action of the liquid composition on various materials was investigated.
In the same procedure as in Test Example 1, a liquid composition (caprylic acid monoglyceride 0.15%, phytic acid 0.40%, ethanol 40.00%, water residue; unit is mass%; pH 2.00), and sample bacteria A liquid was prepared.
As target materials, metal (stainless steel plate), rubber (rubber gloves), and plastic (guard cover) were prepared. The stainless steel plate and watch cover were cut into 10 cm squares. The rubber gloves were stretched on a 10 cm square plastic plate so as not to be wrinkled and attached with double-sided tape, and the remaining part was cut and used.
菌数の測定は、各素材につき以下の手順で行った。各対象素材を３群に分けた。第一の群では、液状組成物０．５ｍＬをコンラージ棒で対象素材の上に均一に塗布し、次いでサンプル菌液０．１ｍＬを接種し、４０秒間コンラージ棒で混合しながら塗り広げた（０ｈ群）。残りの２群では、液状組成物０．５ｍＬ又は１．０ｍＬを、コンラージ棒で対象素材の上に均一に塗布した後、安全キャビネット内で１８時間風乾し、水分とエタノールを完全に蒸発させた。これにサンプル菌液１．０ｍＬを接種し、４０秒間コンラージ棒で混合しながら塗り広げた（１８ｈ群）。サンプル初発菌数は１０5～１０6ｃｆｕ/ｍＬに調整した。
サンプル菌液を塗布した対象素材を５分間静置後、無菌パウチに入れ、上から滅菌水１０ｍＬを加え、熱シールして上下に５回程度軽く振った。パウチを開封して中の液体を採取し、ＭＲＳ寒天培地で菌数を測定し、試験例１と同様の基準で殺菌又は静菌特性を評価した。
The number of bacteria was measured according to the following procedure for each material. Each target material was divided into 3 groups. In the first group, 0.5 mL of the liquid composition was evenly applied onto the target material with a large rod, then 0.1 mL of the sample bacterial solution was inoculated, and spread with mixing with a large rod for 40 seconds (0 h group). In the remaining two groups, 0.5 mL or 1.0 mL of the liquid composition was evenly applied onto the target material with a large stick and then air-dried in a safety cabinet for 18 hours to completely evaporate moisture and ethanol. . This was inoculated with 1.0 mL of the sample bacterial solution, and spread with mixing with a large rod for 40 seconds (group 18h). The initial bacterial count of the sample was adjusted to 10 5 to 10 6 cfu / mL.
The target material to which the sample bacterial solution was applied was allowed to stand for 5 minutes, then placed in a sterile pouch, added with 10 mL of sterilized water from above, heat-sealed, and gently shaken up and down about 5 times. The pouch was opened, the liquid inside was collected, the number of bacteria was measured with MRS agar medium, and bactericidal or bacteriostatic properties were evaluated according to the same criteria as in Test Example 1.
結果を表６に示す。本発明の組成物が、塗布直後（０ｈ群）及び塗布後時間が経過した場合（１８ｈ群）のいずれにおいても、各種素材の殺菌又は静菌に有効であることが分かった。 The results are shown in Table 6. It was found that the composition of the present invention is effective for sterilization or bacteriostatic of various materials both immediately after application (0 h group) and when the time after application has elapsed (18 h group).
Claims (38)
該液状組成物が、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である、
殺菌又は静菌方法。 Spraying or applying the liquid composition to the subject, or immersing the subject in the liquid composition;
The liquid composition contains an emulsifier and phytic acid and has a pH of 3.5 or less.
Sterilization or bacteriostatic method.
該液状組成物が、乳化剤とフィチン酸とを含み、かつｐＨ３．５以下である、
対象への抗菌性付与方法。 Spraying or applying the liquid composition to the subject, or immersing the subject in the liquid composition;
The liquid composition contains an emulsifier and phytic acid and has a pH of 3.5 or less.
A method for imparting antibacterial properties to subjects.
The use according to any one of claims 22 to 37, wherein the liquid composition further comprises an aqueous solvent.
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| KR102208456B1 (en) * | 2020-08-07 | 2021-01-27 | 주식회사 세니젠 | Antimicrobial composition for washing fruit and vegetable |
| KR102447491B1 (en) * | 2022-01-17 | 2022-09-27 | 주식회사 세니젠 | Composition for killing endospores comprising hypochlorous acid and phytic acid |
| CN115413749A (en) * | 2022-08-09 | 2022-12-02 | 大连民族大学 | Atmospheric pressure-cooled plasma preparation method and application of a high-efficiency antibacterial active oil-water emulsion |
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| CN115413749B (en) * | 2022-08-09 | 2024-01-05 | 大连民族大学 | Atmospheric pressure cold plasma preparation method and application of highly effective antibacterial active oil-water emulsion |
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