KR102208456B1 - Antimicrobial composition for washing fruit and vegetable - Google Patents
Antimicrobial composition for washing fruit and vegetable Download PDFInfo
- Publication number
- KR102208456B1 KR102208456B1 KR1020200098973A KR20200098973A KR102208456B1 KR 102208456 B1 KR102208456 B1 KR 102208456B1 KR 1020200098973 A KR1020200098973 A KR 1020200098973A KR 20200098973 A KR20200098973 A KR 20200098973A KR 102208456 B1 KR102208456 B1 KR 102208456B1
- Authority
- KR
- South Korea
- Prior art keywords
- surfactant
- phytic acid
- antimicrobial composition
- sodium hypochlorite
- vegetables
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 22
- 235000012055 fruits and vegetables Nutrition 0.000 title claims abstract description 16
- 230000000845 anti-microbial effect Effects 0.000 title claims abstract description 13
- 238000005406 washing Methods 0.000 title abstract description 4
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 32
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims abstract description 31
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000000467 phytic acid Substances 0.000 claims abstract description 31
- 229940068041 phytic acid Drugs 0.000 claims abstract description 31
- 235000002949 phytic acid Nutrition 0.000 claims abstract description 31
- 239000004094 surface-active agent Substances 0.000 claims abstract description 23
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 18
- 229910019093 NaOCl Inorganic materials 0.000 claims abstract description 14
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 11
- 229930195729 fatty acid Natural products 0.000 claims abstract description 11
- 239000000194 fatty acid Substances 0.000 claims abstract description 11
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 11
- 235000011187 glycerol Nutrition 0.000 claims abstract description 11
- 229920000728 polyester Polymers 0.000 claims abstract description 11
- 239000004599 antimicrobial Substances 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 19
- 239000000645 desinfectant Substances 0.000 abstract description 13
- 244000005700 microbiome Species 0.000 abstract description 13
- 230000000249 desinfective effect Effects 0.000 abstract description 11
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 abstract description 7
- 238000009792 diffusion process Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 26
- 239000000460 chlorine Substances 0.000 description 16
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 11
- 229910052801 chlorine Inorganic materials 0.000 description 11
- 230000002779 inactivation Effects 0.000 description 10
- 230000001954 sterilising effect Effects 0.000 description 8
- 241000193830 Bacillus <bacterium> Species 0.000 description 7
- 230000000813 microbial effect Effects 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 238000012790 confirmation Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 239000012190 activator Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000013011 aqueous formulation Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- -1 hypochlorite ions Chemical class 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
본 발명은 차아염소산 나트륨(NaOCl), 피트산(phytic acid) 및 계면활성제를 포함하는 과채류 세척용 항균제 조성물에 관한 것으로, 차아염소산나트륨(NaOCl)에 피트산 및 계면활성제(폴리에스테르지방산글리세린)을 혼합하면, 피트산의 pH 조절 효과 및 HOCl의 세포 확산 증대로 인해 소독능 극대화되고, 계면활성제로 인해 표면에서 미생물과 소독제의 접촉 증가하여, 과채류의 표면 소독 효율이 향상됨을 확인할 수 있었다.The present invention relates to an antimicrobial composition for washing fruits and vegetables comprising sodium hypochlorite (NaOCl), phytic acid and a surfactant, wherein phytic acid and a surfactant (polyester fatty acid glycerin) are added to sodium hypochlorite (NaOCl). When mixed, it was confirmed that the disinfecting ability was maximized due to the pH control effect of phytic acid and increased cell diffusion of HOCl, and the contact of microorganisms and the disinfectant on the surface increased due to the surfactant, thereby improving the surface disinfection efficiency of fruits and vegetables.
Description
본 발명은 과채류 세척용 항균제 조성물에 관한 것으로, 더욱 상세하게는 차아염소산 나트륨(NaOCl), 피트산(phytic acid) 및 계면활성제를 포함하는 과채류 세척용 항균제 조성물에 관한 것이다. The present invention relates to an antibacterial composition for washing fruits and vegetables, and more particularly, to an antimicrobial composition for washing fruits and vegetables comprising sodium hypochlorite (NaOCl), phytic acid, and a surfactant.
위생에 대한 관심의 증가와 농업 용수의 오염으로 인해 과채류를 단순 세척할 뿐만 아니라 소독할 필요성이 증가하고 있다. 일반적인 염소 소독제의 경우, 소독 효율이 떨어지며 포자류를 제어하는데 한계를 가지고 있다. pH를 조절할 경우 염소 소독 효율이 높아지기는 하지만, 소독 효율이 pH에 따라 급격하게 바뀌는 문제가 있다. 따라서, 세척 과정 또는 세척수와 혼합되며, pH에 따른 소독 효율의 변화가 없는 세척 및 살균소독제가 필요한 실정이다.Due to the growing interest in hygiene and pollution of agricultural water, the need to disinfect fruits and vegetables as well as simple cleaning is increasing. In the case of general chlorine disinfectants, disinfection efficiency is inferior and has limitations in controlling spores. If the pH is adjusted, the chlorine disinfection efficiency increases, but there is a problem that the disinfection efficiency rapidly changes depending on the pH. Therefore, there is a need for a cleaning and sterilizing disinfectant that is mixed with the cleaning process or the cleaning water and does not change the disinfection efficiency according to the pH.
본 발명에서는 pH 변화에도 살균능의 변화가 없으며, 과채류의 살균에 적합한 염소계 살균소독제를 개발하여 제공하고자 한다. In the present invention, there is no change in sterilization ability even with a change in pH, and an object of the present invention is to develop and provide a chlorine-based disinfectant suitable for sterilization of fruits and vegetables.
본 발명은 차아염소산 나트륨(NaOCl), 피트산(phytic acid) 및 계면활성제를 포함하는 항균제 조성물을 제공한다. The present invention provides an antimicrobial composition comprising sodium hypochlorite (NaOCl), phytic acid, and a surfactant.
본 발명의 항균제 조성물에 있어서, 상기 항균제 조성물은, 바람직하게 과채류 표면 세척용일 수 있다. In the antimicrobial composition of the present invention, the antimicrobial composition may be preferably for cleaning the surface of fruits and vegetables.
본 발명의 항균제 조성물에 있어서, 상기 계면활성제는, 바람직하게 폴리에스테르지방산글리세린일 수 있다. In the antimicrobial composition of the present invention, the surfactant may preferably be glycerin polyester fatty acid.
본 발명에서는 차아염소산나트륨(NaOCl)에 피트산 및 계면활성제(폴리에스테르지방산글리세린)을 혼합하면, 피트산의 pH 조절 효과 및 HOCl의 세포 확산 증대로 인해 소독능 극대화되고, 계면활성제로 인해 수중에 있는 과채류 표면에서 미생물과 소독제의 접촉 가능성을 증가시켜, 과채류의 표면 소독 효율이 향상됨을 확인할 수 있었다. In the present invention, when phytic acid and a surfactant (polyester fatty acid glycerin) are mixed with sodium hypochlorite (NaOCl), disinfecting power is maximized due to the pH adjustment effect of phytic acid and increased cell diffusion of HOCl, and in water due to the surfactant It was confirmed that the surface disinfection efficiency of fruits and vegetables was improved by increasing the possibility of contact between microorganisms and disinfectants on the surface of fruits and vegetables.
도 1은 차아염소산나트륨(NaOCl) 용해시, pH 범위에 따른 Cl2, HOCl 및 OCl-의 비율을 보여준다.
도 2는 피트산의 첨가에 따른 NaOCl의 pH 3~7에서 소독능(바실러스 포자 불활성화) 변화를 보여주는 결과이다.
도 3은 피트산 첨가에 따른 미생물 표면 구조 붕괴를 확인시켜 주는 PI 염색 결과이다.
도 4는 계면활성제의 첨가에 따른 소독능 (바실러스 포자 불활성화) 증대를 보여준다.1 shows the ratio of Cl 2 , HOCl and OCl - according to the pH range when sodium hypochlorite (NaOCl) is dissolved.
2 is a result showing a change in disinfecting ability (inactivation of Bacillus spores) at pH 3-7 of NaOCl according to the addition of phytic acid.
3 is a PI staining result confirming the collapse of the microbial surface structure according to the addition of phytic acid.
4 shows an increase in disinfecting ability (inactivation of Bacillus spores) according to the addition of a surfactant.
본 발명은 차아염소산 나트륨(NaOCl), 피트산(phytic acid) 및 계면활성제를 포함하는 항균제 조성물을 제공한다. The present invention provides an antimicrobial composition comprising sodium hypochlorite (NaOCl), phytic acid, and a surfactant.
본 발명의 차아염소산 나트륨은 수중에 용해되면 pH에 따라 Cl2, HOCl, OCl-로 존재한다. 이중, 차아염소산(HOCl)은 생물 내부로 확산(diffusion)되어 미생물을 불활성화시키는 역할을 수행한다. 하지만, 도 1에서 보는 바와 같이 pH 4~6에서만 차아염소산 형태로 유지되고, pH8 이상에서는 OCl-로 존재한다. 또한 pH 2 이하에서는 Cl2 형태로 존재한다. HOCl, OCl- 중에서 차아염소산(HOCl)의 소독 효율이 차아염소산이온(OCl-) 보다 수십 배에서 수백 배 높은 것으로 보고되고 있다, 하지만, 차아염소산은 pH 변화에 따라 민감하기 때문에, pH 변화에도 안정적으로 살균능을 발휘하는 조성물을 개발할 필요가 있는 것이다. When the sodium hypochlorite of the present invention is dissolved in water, it exists as Cl 2 , HOCl, OCl - depending on the pH. Among them, hypochlorous acid (HOCl) plays a role of inactivating microorganisms by diffusion into living organisms. However, as shown in Fig. 1, it is maintained in the form of hypochlorous acid only at
본 발명에서는 피트산의 첨가에 의해 상기 문제를 해결하였는데, 피트산은 미생물 세포막을 붕괴시켜 차아염소산(HOCl)이 내부로 손쉽게 확산되게 한다. 즉, 피트산은 미생물 표면 구조(surface components)를 붕괴시키는 역할을 하는데, 이를 통해 광범위한 pH 범위에서도 미생물을 불활성화시킬 수 있음을 본 발명을 통해 확인할 수 있었다. 즉, 피트산을 첨가할 경우, pH 3~7의 영역에서 일정 수준 (99.9% 이상)의 미생물 불활성화를 확인할 수 있었다. 특히나, 바실러스 포자를 불활성화시킬 수 있음을 확인할 수 있었다. In the present invention, the above problem was solved by the addition of phytic acid, but phytic acid disrupts microbial cell membranes so that hypochlorous acid (HOCl) can easily diffuse into the interior. That is, it was confirmed through the present invention that phytic acid serves to disrupt microbial surface components, through which microbes can be inactivated even in a wide pH range. That is, when phytic acid was added, it was possible to confirm the inactivation of microorganisms at a certain level (99.9% or more) in the range of pH 3-7. In particular, it was confirmed that Bacillus spores can be inactivated.
한편, 액상 내의 미생물을 소독하는 경우와 달리 과채류처럼 표면을 소독할 경우, 소독제와 미생물 간의 접촉이 원활하지 않아 일정 수준 이상의 처리가 어렵게 된다. 그런데, 본 발명의 조성물에 계면활성제를 첨가할 경우, 소독제와 미생물 간의 접촉을 증대시켜 더 높은 수준의 소독이 가능해짐을 본 발명에서 확인할 수 있었다. 계면활성제로는 바람직하게 폴리에스테르지방산글리세린을 사용할 수 있다. On the other hand, unlike the case of disinfecting microorganisms in the liquid phase, when the surface is disinfected like fruits and vegetables, the contact between the disinfectant and the microorganisms is not smooth, making it difficult to process more than a certain level. However, it was confirmed in the present invention that when a surfactant is added to the composition of the present invention, a higher level of disinfection is possible by increasing the contact between the disinfectant and the microorganism. As the surfactant, it is preferable to use glycerin polyester fatty acid.
본 발명의 조성물은, 수용액상의 제형으로 제조될 때, 바람직하게 차아염소산 나트륨(NaOCl)을 50.4~100.8 mg/L, 피트산을 60~180 mg/L, 계면활성제 (바람직하게 상온에서 액상인 계면활성제. 일 예로, 폴리에스테르지방산글리세린)를 300~700 μl/L의 비율로 전체 수용액상 조성물 내에 포함하는 것이 좋다. When the composition of the present invention is prepared in an aqueous formulation, preferably 50.4 to 100.8 mg/L of sodium hypochlorite (NaOCl), 60 to 180 mg/L of phytic acid, a surfactant (preferably liquid at room temperature) Activator, for example, polyester fatty acid glycerin) is preferably included in the total aqueous composition at a rate of 300 to 700 μl/L.
더욱 바람직하게, 차아염소산 나트륨(NaOCl)은 차아염소산 나트륨을 12%(w/v) 함유하는 수용액 상태로 전체 조성물에 420~840 μl/L 첨가되는 것이 좋고, 피트산(phytic acid)은 피트산을 50%(w/v) 함유하는 수용액 상태로 전체 조성물에 120~360 μl/L 첨가되는 것이 좋으며, 계면활성제 (바람직하게 상온에서 액상인 계면활성제. 일 예로, 폴리에스테르지방산글리세린)은 300~700 μl/L의 비율로 본 발명의 전체 조성물에 첨가되는 것이 좋다. More preferably, sodium hypochlorite (NaOCl) is preferably added 420 to 840 μl/L to the total composition in an aqueous solution containing 12% (w/v) sodium hypochlorite, and phytic acid is phytic acid. It is recommended to add 120 to 360 μl/L to the total composition in an aqueous solution containing 50% (w/v), and a surfactant (preferably a liquid surfactant at room temperature. For example, polyester fatty acid glycerin) is 300 to It is preferably added to the total composition of the present invention at a rate of 700 μl/L.
본 발명의 항균제 조성물은 상기 세가지 주요 성분 외에 기타 보조제를 포함할 수도 있다. The antimicrobial composition of the present invention may contain other adjuvants in addition to the three main components.
이하, 본 발명의 내용을 하기 실시예 및 실험예를 통해 더욱 상세히 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예 및 실험예에만 한정되는 것은 아니고, 그와 등가의 기술적 사상의 변형까지를 포함한다. Hereinafter, the content of the present invention will be described in more detail through the following examples and experimental examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes modifications of equivalent technical ideas.
[실험예 1: 차아염소산 나트륨에 피트산 첨가에 따른 바실러스 포자의 불활성화 증가 확인] [Experimental Example 1: Confirmation of increased inactivation of Bacillus spores by adding phytic acid to sodium hypochlorite]
(1) pH 범위에 따른 염소의 분포(1) Distribution of chlorine according to the pH range
차아염소산나트륨을 수중에 용해하면 유리염소(Free chlorine=HOCl, OCl-)은 pH에 따라 Cl2, HOCl, OCl-로 존재하게 되는데, 차아염소산(HOCl)의 소독 효율이 차아염소산이온(OCl-) 보다 수십 배에서 수백 배 높은 것으로 보고되고 있다 (도 1). 하지만, 도 1에서 보는 바와 같이 pH 4~6에서만 차아염소산 형태로 유지되고, pH8 이상에서는 OCl-로 존재한다. 또한 pH 2 이하에서는 Cl2 형태로 존재한다. 따라서, 차아염소산나트륨을 포함하는 소독제의 소독 효율은 pH 범위에 따라 급속하게 변하게 된다. When dissolving the same procedure as set forth in the hands free chlorine (Free chlorine = HOCl, OCl - ) are Cl 2, HOCl, OCl according to pH - there is exists as hypochlorite (HOCl) disinfection efficiency of hypochlorite ions (OCl - ), it is reported to be several tens of times higher than that of (Fig. 1). However, as shown in Fig. 1, it is maintained in the form of hypochlorous acid only at
(2) 차아염소산 나트륨과 피트산의 시너지 효과(2) Synergistic effect of sodium hypochlorite and phytic acid
50 mg/L의 유리염소에 5분간 노출시킨 경우에도 pH 5에서는 99.9% 이상의 바실러스 포자의 불활성화가 확인됐지만, pH 3에서는 거의 불활성화가 나타나지 않았고, pH 7에서는 85%의 불활성화만 보였다. 이에 반해, 피트산을 첨가할 경우, pH 3~7의 영역에서 일정 수준 (99.9% 이상)의 미생물 불활성화를 확인할 수 있었다 (도 2). 도 2는 피트산의 첨가에 따른 NaOCl의 pH 3~7에서 소독능(바실러스 포자 불활성화) 변화를 보여주는 결과이다. Even when exposed to 50 mg/L of free chlorine for 5 minutes, more than 99.9% of Bacillus spores were inactivated at
[실험예 2: 피트산 첨가에 따른 미생물 표면 구조 붕괴 확인][Experimental Example 2: Confirmation of microbial surface structure collapse due to addition of phytic acid]
PI (propidiumiodide) 시약은 세포 내부의 핵을 염색하는 형광염료로, 손상받지 않은 세포를 통과할 수 없기 때문에, PI 염색 실험은 미생물 표면 구조의 붕괴를 확인하는 대표적인 지표 물질이다. 유리염소 단독 샘플 처리에 비해 피트산을 넣을 경우 PI 염색 정도가 증가하여, 표면이 파괴됨을 확인할 수 있었다 (도 3) (시너지 효과). 도 3은 피트산 첨가에 따른 미생물 표면 구조 붕괴를 확인시켜 주는 PI 염색 결과이다. PI (propidiumiodide) reagent is a fluorescent dye that stains the inner nucleus of cells, and because it cannot pass through undamaged cells, PI staining experiments are a representative indicator of disruption of the microbial surface structure. Compared to the free chlorine alone sample treatment, when phytic acid was added, the degree of PI staining increased, and it was confirmed that the surface was destroyed (FIG. 3) (synergy effect). 3 is a PI staining result confirming the collapse of the microbial surface structure according to the addition of phytic acid.
실험 결과, 피트산의 첨가에 의해 미생물 표면 구조가 더 붕괴됨을 확인할 수 있었다.As a result of the experiment, it was confirmed that the microbial surface structure was further destroyed by the addition of phytic acid.
[실험예 3: 계면활성제 첨가에 따른 항균(소독) 효능 증진 확인][Experimental Example 3: Confirmation of improvement of antibacterial (disinfection) efficacy by addition of surfactant]
액상 내의 미생물을 소독하는 경우와 달리 과채류처럼 표면을 소독할 경우, 소독제와 미생물 간의 접촉이 원활하지 않아 일정 수준 이상의 처리가 어렵게 된다. 이때, 미량의 계면활성제 (식품첨가물 등급)를 첨가할 경우, 소독제와 미생물 간의 접촉을 증대시켜 더 높은 수준의 소독이 가능해짐을 본 발명을 통해 확인할 수 있었다. Unlike in the case of disinfecting microorganisms in a liquid phase, when the surface is disinfected like fruits and vegetables, the contact between the disinfectant and the microorganisms is not smooth, making it difficult to treat more than a certain level. At this time, it was confirmed through the present invention that when a trace amount of surfactant (food additive grade) is added, a higher level of disinfection is possible by increasing the contact between the disinfectant and the microorganism.
유리염소 및 피트산으로 표면을 소독할 경우, 위의 결과처럼 일정 수준 이상의 불활성화는 일어나는 것을 확인할 수 있었다 (4분에서 99.54%, 8분에서 99.62%). 하지만, 0.05%의 계면활성제(폴리에스테르지방산글리세린)를 첨가할 경우, 표면의 미생물과 소독제의 접촉이 증가되어 더 높은 수준의 불활성화가 확인되었으며, 특히, 시간에 따라 소독 수준이 증가 되었음을 확인할 수 있었다 (4분에서 99.85%, 8분에서 99.97%) (도 4). 도 4는 계면활성제의 첨가에 따른 소독능 (바실러스 포자 불활성화) 증대를 보여준다.In the case of disinfecting the surface with free chlorine and phytic acid, it was confirmed that more than a certain level of inactivation occurred (99.54% in 4 minutes, 99.62% in 8 minutes) as shown above. However, when 0.05% of surfactant (polyester fatty acid glycerin) was added, the contact between the microorganisms on the surface and the disinfectant was increased, and a higher level of inactivation was confirmed.In particular, it was confirmed that the level of disinfection increased with time. Yes (99.85% at 4 minutes, 99.97% at 8 minutes) (Fig. 4). 4 shows an increase in disinfecting ability (inactivation of Bacillus spores) according to the addition of a surfactant.
즉, 차아염소산나트륨(NaOCl)에 피트산 및 계면활성제(폴리에스테르지방산글리세린)를 혼합하면, 피트산의 pH 조절 효과 및 HOCl의 세포 확산 증대로 인해 소독능 극대화되고, 계면활성제로 인해 표면에서 미생물과 소독제의 접촉이 증가하여 과채류 표면 소독 효율이 향상됨을 확인할 수 있었던 것이다.That is, when phytic acid and surfactant (polyester fatty acid glycerin) are mixed with sodium hypochlorite (NaOCl), disinfecting power is maximized due to the pH control effect of phytic acid and increased cell diffusion of HOCl, and microorganisms on the surface due to surfactant It was confirmed that the efficiency of disinfecting the surface of fruits and vegetables was improved by increasing the contact of the disinfectant.
[실시예 1: 차아염소산 나트륨, 피트산 및 폴리에스테르지방산글리세린을 포함하는 본 발명 항균 조성물의 제조][Example 1: Preparation of an antimicrobial composition of the present invention containing sodium hypochlorite, phytic acid and glycerin polyester fatty acid]
차아염소산 나트륨(NaOCl) 12%(w/v) 함유 수용액 630 μl/L, 피트산(phytic acid) 50%(w/v) 함유 수용액 240 μl/L, 폴리에스테르지방산글리세린 500 μl/L을 정제수에 넣어 본 발명의 항균 조성물을 제조하였다. Sodium hypochlorite (NaOCl) 12% (w/v) aqueous solution 630 μl/L, phytic acid 50% (w/v) aqueous solution 240 μl/L, polyester
Claims (3)
상기 계면활성제는 폴리에스테르지방산글리세린인 것을 특징으로 하는 항균제 조성물.
Contains sodium hypochlorite (NaOCl), phytic acid and surfactant,
The surfactant is an antimicrobial composition, characterized in that the polyester fatty acid glycerin.
상기 항균제 조성물은,
과채류 표면 세척용인 것을 특징으로 하는 항균제 조성물.
The method of claim 1,
The antimicrobial composition,
Antimicrobial composition, characterized in that for cleaning the surface of fruits and vegetables.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200098973A KR102208456B1 (en) | 2020-08-07 | 2020-08-07 | Antimicrobial composition for washing fruit and vegetable |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200098973A KR102208456B1 (en) | 2020-08-07 | 2020-08-07 | Antimicrobial composition for washing fruit and vegetable |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR102208456B1 true KR102208456B1 (en) | 2021-01-27 |
Family
ID=74238404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020200098973A Active KR102208456B1 (en) | 2020-08-07 | 2020-08-07 | Antimicrobial composition for washing fruit and vegetable |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR102208456B1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102447491B1 (en) | 2022-01-17 | 2022-09-27 | 주식회사 세니젠 | Composition for killing endospores comprising hypochlorous acid and phytic acid |
| KR102536412B1 (en) * | 2022-01-26 | 2023-05-26 | 주식회사 세니젠 | Apparatus and method for producing a composition for killing endospores containing hypochlorous acid and phytic acid |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020065902A (en) * | 1999-12-10 | 2002-08-14 | 가오가부시끼가이샤 | Methods of sterilization |
| KR20020065900A (en) * | 1999-12-10 | 2002-08-14 | 가오가부시끼가이샤 | Microbicide compositions |
| JP2010057429A (en) * | 2008-09-04 | 2010-03-18 | Okuno Chem Ind Co Ltd | Method for sterilizing food |
| KR20100136845A (en) | 2009-06-19 | 2010-12-29 | 한국식품연구원 | Combined sterilization method of fruit vegetable using chlorine disinfectant |
| KR20170004800A (en) * | 2015-07-01 | 2017-01-11 | 고려대학교 산학협력단 | Antimicrobial composition comprising phytic acid and electrolytes |
| WO2018168977A1 (en) * | 2017-03-16 | 2018-09-20 | 株式会社日清製粉グループ本社 | Liquid composition for bacteriostasis or sterilization |
| KR101976709B1 (en) * | 2018-08-08 | 2019-05-09 | 주식회사 세니젠 | Antimicrobial composition with malic acid, citric acid and phytic acid |
-
2020
- 2020-08-07 KR KR1020200098973A patent/KR102208456B1/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020065902A (en) * | 1999-12-10 | 2002-08-14 | 가오가부시끼가이샤 | Methods of sterilization |
| KR20020065900A (en) * | 1999-12-10 | 2002-08-14 | 가오가부시끼가이샤 | Microbicide compositions |
| JP2010057429A (en) * | 2008-09-04 | 2010-03-18 | Okuno Chem Ind Co Ltd | Method for sterilizing food |
| KR20100136845A (en) | 2009-06-19 | 2010-12-29 | 한국식품연구원 | Combined sterilization method of fruit vegetable using chlorine disinfectant |
| KR20170004800A (en) * | 2015-07-01 | 2017-01-11 | 고려대학교 산학협력단 | Antimicrobial composition comprising phytic acid and electrolytes |
| WO2018168977A1 (en) * | 2017-03-16 | 2018-09-20 | 株式会社日清製粉グループ本社 | Liquid composition for bacteriostasis or sterilization |
| KR101976709B1 (en) * | 2018-08-08 | 2019-05-09 | 주식회사 세니젠 | Antimicrobial composition with malic acid, citric acid and phytic acid |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102447491B1 (en) | 2022-01-17 | 2022-09-27 | 주식회사 세니젠 | Composition for killing endospores comprising hypochlorous acid and phytic acid |
| WO2023136704A1 (en) * | 2022-01-17 | 2023-07-20 | 주식회사 세니젠 | Composition for killing endospores comprising slightly weak acidic hypochlorous acid water and phytic acid |
| KR102536412B1 (en) * | 2022-01-26 | 2023-05-26 | 주식회사 세니젠 | Apparatus and method for producing a composition for killing endospores containing hypochlorous acid and phytic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Al-Haq et al. | Applications of electrolyzed water in agriculture & food industries | |
| Rahman et al. | Electrolyzed water as a novel sanitizer in the food industry: current trends and future perspectives | |
| Quan et al. | Evaluation of bactericidal activity of weakly acidic electrolyzed water (WAEW) against Vibrio vulnificus and Vibrio parahaemolyticus | |
| Al-Haq et al. | Disinfection effects of electrolyzed oxidizing water on suppressing fruit rot of pear caused by Botryosphaeria berengeriana | |
| RU2469537C2 (en) | Electrochemical device for biocidal treatments in agricultural use | |
| Naka et al. | Effectiveness of slightly acidic electrolyzed water on bacteria reduction: in vitro and spray evaluation | |
| KR102208456B1 (en) | Antimicrobial composition for washing fruit and vegetable | |
| JPH1081610A (en) | Production of sterilizing antispetic solution and sterilizing disinfectant | |
| Turantaş et al. | Decontamination effect of electrolyzed water washing on fruits and vegetables | |
| US10993448B2 (en) | Method for sanitizing fresh produce | |
| KR101297712B1 (en) | Biocide/disinfection agent containing hypochlorous acid water and soy protein | |
| KR101085458B1 (en) | Combined sterilization method of fruit vegetable using chlorine disinfectant | |
| KR20140065566A (en) | Composition containing slightly acidic hypochlorous water for plant diseases and method for controlling plant diseases using the same | |
| US6331514B1 (en) | Sterilizing and disinfecting compound | |
| KR100363896B1 (en) | A medical instrument cleaning solution using peracetic acid and the manufacturing method of the above solution | |
| KR101848657B1 (en) | Environmentally friendly sterilizing water comprising slightly acidic electrolyzed water and a sterilizing method establishing sterilization condition for fresh agricultural products by using the same | |
| JPH11226579A (en) | Sterilizing method and apparatus | |
| Chmielewski et al. | Inactivation of Listeria monocytogenes biofilms using chemical sanitizers and heat | |
| JP2011516411A (en) | Disinfecting composition | |
| Konopka et al. | Disinfection of meat industry equipment and production rooms with the use of liquids containing silver nano-particles | |
| Popova | Disinfection of Fruits with Activated Water | |
| Nguyen et al. | Physicochemical properties and bactericidal effects of neutral electrolyzed water against Escherichia coli and Salmonella enterica on ground pork | |
| Issa-Zacharia | Microbial Inactivation Mechanism and Properties of Slightly Acidic Electrolyzed Water: A Review | |
| Chiu et al. | The relevance of electrolyzed water to infectious and chronic diseases | |
| Seham et al. | A trial to sustain the safety of chicken fillet using neutral electrolyzed water (new) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 20200807 |
|
| PA0201 | Request for examination | ||
| PA0302 | Request for accelerated examination |
Patent event date: 20200819 Patent event code: PA03022R01D Comment text: Request for Accelerated Examination Patent event date: 20200807 Patent event code: PA03021R01I Comment text: Patent Application |
|
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20201002 Patent event code: PE09021S01D |
|
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 20210114 |
|
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 20210121 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 20210121 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration | ||
| PR1001 | Payment of annual fee |
Payment date: 20250106 Start annual number: 5 End annual number: 5 |