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WO2008096757A1 - 生体分子検出方法及び生体分子検出用チップ - Google Patents

生体分子検出方法及び生体分子検出用チップ Download PDF

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Publication number
WO2008096757A1
WO2008096757A1 PCT/JP2008/051867 JP2008051867W WO2008096757A1 WO 2008096757 A1 WO2008096757 A1 WO 2008096757A1 JP 2008051867 W JP2008051867 W JP 2008051867W WO 2008096757 A1 WO2008096757 A1 WO 2008096757A1
Authority
WO
WIPO (PCT)
Prior art keywords
biological molecule
detection
electrochemical response
measurement
measuring
Prior art date
Application number
PCT/JP2008/051867
Other languages
English (en)
French (fr)
Inventor
Masato Nakayama
Original Assignee
Toppan Printing Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toppan Printing Co., Ltd. filed Critical Toppan Printing Co., Ltd.
Priority to JP2008557124A priority Critical patent/JP5262725B2/ja
Priority to EP08710799A priority patent/EP2110662A4/en
Priority to US12/449,373 priority patent/US20100320096A1/en
Publication of WO2008096757A1 publication Critical patent/WO2008096757A1/ja
Priority to US13/323,107 priority patent/US20120080312A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　電気化学的応答の測定により検出対象生体分子を検出する生体分子検出方法であって、前記検出対象生体分子に対して直接的あるいは間接的に結合性を有する電気化学的活性物質を含む基準溶液の前記電気化学的応答を測定する基準溶液測定ステップと、前記検出対象生体分子を含む被検試料と前記電気化学的活性物質とを含む被検溶液の電気化学的応答を測定する被検溶液測定ステップと、前記基準溶液の電気化学的応答の測定結果と前記被検溶液の電気化学的応答の測定結果とを比較して比較結果を導出する比較ステップと、前記比較結果を基に前記被検溶液中の前記検出対象生体分子の含有情報を検出する生体分子含有情報検出ステップとを含む生体分子検出方法。
PCT/JP2008/051867 2007-02-06 2008-02-05 生体分子検出方法及び生体分子検出用チップ WO2008096757A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2008557124A JP5262725B2 (ja) 2007-02-06 2008-02-05 核酸検出方法及び核酸検出用チップ
EP08710799A EP2110662A4 (en) 2007-02-06 2008-02-05 PROCESS AND CHIP FOR DETECTION OF A BIOLOGICAL MOLECULE
US12/449,373 US20100320096A1 (en) 2007-02-06 2008-02-05 Biomolecules detection method and biomolecules detection chip
US13/323,107 US20120080312A1 (en) 2007-02-06 2011-12-12 Biomolecules detection method and biomolecules detection chip

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-027222 2007-02-06
JP2007027222 2007-02-06

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/323,107 Division US20120080312A1 (en) 2007-02-06 2011-12-12 Biomolecules detection method and biomolecules detection chip

Publications (1)

Publication Number Publication Date
WO2008096757A1 true WO2008096757A1 (ja) 2008-08-14

Family

ID=39681665

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/051867 WO2008096757A1 (ja) 2007-02-06 2008-02-05 生体分子検出方法及び生体分子検出用チップ

Country Status (5)

Country Link
US (2) US20100320096A1 (ja)
EP (1) EP2110662A4 (ja)
JP (1) JP5262725B2 (ja)
CN (1) CN101606057A (ja)
WO (1) WO2008096757A1 (ja)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140154668A1 (en) 2010-05-21 2014-06-05 The Trustees Of Princeton University Structures for Enhancement of Local Electric Field, Light Absorption, Light Radiation, Material Detection and Methods for Making and Using of the Same.
EP2836821B1 (en) * 2012-04-10 2018-09-26 The Trustees of Princeton University Ultra-sensitive sensor
CN102621133B (zh) * 2012-04-17 2016-08-03 北京师范大学 一种基于光学dna生物传感器的1,8-二氨基萘的检测方法
US20230054407A1 (en) * 2020-01-13 2023-02-23 Arizona Board Of Regents On Behalf Of The University Of Arizona Optical biopsy stain panels and methods of use

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3511596B2 (ja) 2001-01-25 2004-03-29 栄一 民谷 遺伝子の検出方法及び検出用チップ
JP2005518810A (ja) * 2002-03-07 2005-06-30 モレキュラ センシング ピーエルシー 核酸プローブとその合成および使用
JP2006504936A (ja) * 2002-10-30 2006-02-09 ラジオメーター・メディカル・アー・ペー・エス センサーの較正及び品質制御を実行する方法、並びに、該方法を実行する装置
JP2007027222A (ja) 2005-07-13 2007-02-01 Sumitomo Bakelite Co Ltd フレキシブルプリント基板

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AU1911795A (en) * 1994-02-09 1995-08-29 Abbott Laboratories Diagnostic flow cell device
CN1483078A (zh) * 2000-10-27 2004-03-17 荣研化学株式会社 合成单链核酸的方法
US6960466B2 (en) * 2001-05-31 2005-11-01 Instrumentation Laboratory Company Composite membrane containing a cross-linked enzyme matrix for a biosensor
US7198754B2 (en) * 2001-08-31 2007-04-03 Kabushiki Kaisha Toshiba Biological material detection element, biological material detection method and apparatus, charged material moving apparatus
US7338760B2 (en) * 2001-10-26 2008-03-04 Ntu Ventures Private Limited Sample preparation integrated chip
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
CN1646911A (zh) * 2002-04-22 2005-07-27 北斗科学产业株式会社 基因检测工具、检测方法以及检测用试剂盒
US7338802B2 (en) * 2002-10-30 2008-03-04 Radiometer Medical Aps Method of performing calibration and quality control of a sensor and apparatus for performing the method
JP2005090976A (ja) * 2003-09-12 2005-04-07 Zoegene Corp 電気泳動用ゲル層担持プレート用アダプタ及び該アダプタを用いた半自動試料分注方法
JP2006145342A (ja) * 2004-11-18 2006-06-08 Eiichi Tamiya ポリヌクレオチドの分析方法
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Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3511596B2 (ja) 2001-01-25 2004-03-29 栄一 民谷 遺伝子の検出方法及び検出用チップ
JP2005518810A (ja) * 2002-03-07 2005-06-30 モレキュラ センシング ピーエルシー 核酸プローブとその合成および使用
JP2006504936A (ja) * 2002-10-30 2006-02-09 ラジオメーター・メディカル・アー・ペー・エス センサーの較正及び品質制御を実行する方法、並びに、該方法を実行する装置
JP2007027222A (ja) 2005-07-13 2007-02-01 Sumitomo Bakelite Co Ltd フレキシブルプリント基板

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KOBAYASHI M. ET AL.: "DNA stick -POC Taiogata DNA Sensor no Kaihatsu-", CHEMICAL SENSORS, vol. 18, no. SUPPL. B, 12 September 2002 (2002-09-12), pages 55 - 57, XP008115591 *
See also references of EP2110662A4 *

Also Published As

Publication number Publication date
US20120080312A1 (en) 2012-04-05
EP2110662A1 (en) 2009-10-21
US20100320096A1 (en) 2010-12-23
JP5262725B2 (ja) 2013-08-14
CN101606057A (zh) 2009-12-16
EP2110662A4 (en) 2012-11-07
JPWO2008096757A1 (ja) 2010-05-20

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