[go: up one dir, main page]

WO2007025097A3 - Utilisation - Google Patents

Utilisation Download PDF

Info

Publication number
WO2007025097A3
WO2007025097A3 PCT/US2006/033167 US2006033167W WO2007025097A3 WO 2007025097 A3 WO2007025097 A3 WO 2007025097A3 US 2006033167 W US2006033167 W US 2006033167W WO 2007025097 A3 WO2007025097 A3 WO 2007025097A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
target nucleic
relates
present
transcription product
Prior art date
Application number
PCT/US2006/033167
Other languages
English (en)
Other versions
WO2007025097A2 (fr
Inventor
Philippe Horvath
Rodolphe Barrangou
Christophe Fremaux
Patrick Boyaval
Dennis Romero
Original Assignee
Danisco
Philippe Horvath
Rodolphe Barrangou
Christophe Fremaux
Patrick Boyaval
Dennis Romero
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37692491&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2007025097(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to CA2619833A priority Critical patent/CA2619833C/fr
Priority to EP17188839.9A priority patent/EP3284833B1/fr
Priority to DE602006015484T priority patent/DE602006015484D1/de
Priority to US11/990,885 priority patent/US10066233B2/en
Priority to DK06802307.6T priority patent/DK1916903T3/da
Priority to AT06802307T priority patent/ATE473637T1/de
Priority to AU2006282983A priority patent/AU2006282983B2/en
Application filed by Danisco, Philippe Horvath, Rodolphe Barrangou, Christophe Fremaux, Patrick Boyaval, Dennis Romero filed Critical Danisco
Priority to EP10169298.6A priority patent/EP2336362B1/fr
Priority to EP06802307A priority patent/EP1916903B1/fr
Priority to NZ565563A priority patent/NZ565563A/en
Priority to NZ597299A priority patent/NZ597299A/xx
Publication of WO2007025097A2 publication Critical patent/WO2007025097A2/fr
Publication of WO2007025097A3 publication Critical patent/WO2007025097A3/fr
Priority to US13/490,050 priority patent/US9879269B2/en
Priority to US13/960,796 priority patent/US20140199767A1/en
Priority to US15/229,103 priority patent/US10640778B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Animal Husbandry (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Communicable Diseases (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne l'utilisation d'un ou de plusieurs gènes cas pour moduler la résistance à l'intérieur d'une cellule vis-à-vis d'un acide nucléique cible ou d'un produit de transcription de celui-ci.
PCT/US2006/033167 2005-08-26 2006-08-25 Utilisation WO2007025097A2 (fr)

Priority Applications (14)

Application Number Priority Date Filing Date Title
NZ597299A NZ597299A (en) 2005-08-26 2006-08-25 Use of a cas gene in combination with CRISPR repeats for modulating resistance in a cell
EP10169298.6A EP2336362B1 (fr) 2005-08-26 2006-08-25 Utilisation de genes associes à crispr (cas)
DE602006015484T DE602006015484D1 (de) 2005-08-26 2006-08-25 Verwendung der crispr assoziierten gene (cas)
US11/990,885 US10066233B2 (en) 2005-08-26 2006-08-25 Method of modulating cell resistance
DK06802307.6T DK1916903T3 (da) 2005-08-26 2006-08-25 Anvendelse af CRISPR-associerede gener (CAS)
AT06802307T ATE473637T1 (de) 2005-08-26 2006-08-25 Verwendung der crispr assoziierten gene (cas)
AU2006282983A AU2006282983B2 (en) 2005-08-26 2006-08-25 Use
CA2619833A CA2619833C (fr) 2005-08-26 2006-08-25 Utilisation de genes associes a crispr-cas
NZ565563A NZ565563A (en) 2005-08-26 2006-08-25 Use of a cas gene in combination with CRISPR repeats for modulating resistance in a cell
EP17188839.9A EP3284833B1 (fr) 2005-08-26 2006-08-25 Utilisation de genes associes à crispr (cas)
EP06802307A EP1916903B1 (fr) 2005-08-26 2006-08-25 Utilisation de genes associes à crispr (cas)
US13/490,050 US9879269B2 (en) 2005-08-26 2012-06-06 Method for modulating resistance
US13/960,796 US20140199767A1 (en) 2005-08-26 2013-08-06 Use
US15/229,103 US10640778B2 (en) 2005-08-26 2016-08-04 Method of modulating cell resistance

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US71139605P 2005-08-26 2005-08-26
US60/711,396 2005-08-26
US74768306P 2006-05-19 2006-05-19
US60/747,683 2006-05-19

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US11/990,885 A-371-Of-International US10066233B2 (en) 2005-08-26 2006-08-25 Method of modulating cell resistance
US13/490,050 Continuation US9879269B2 (en) 2005-08-26 2012-06-06 Method for modulating resistance
US15/229,103 Continuation US10640778B2 (en) 2005-08-26 2016-08-04 Method of modulating cell resistance

Publications (2)

Publication Number Publication Date
WO2007025097A2 WO2007025097A2 (fr) 2007-03-01
WO2007025097A3 true WO2007025097A3 (fr) 2007-07-12

Family

ID=37692491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/033167 WO2007025097A2 (fr) 2005-08-26 2006-08-25 Utilisation

Country Status (12)

Country Link
US (4) US10066233B2 (fr)
EP (5) EP1916903B1 (fr)
AT (1) ATE473637T1 (fr)
AU (1) AU2006282983B2 (fr)
CA (2) CA2619833C (fr)
DE (1) DE602006015484D1 (fr)
DK (5) DK2341149T3 (fr)
ES (1) ES2398918T3 (fr)
NZ (2) NZ565563A (fr)
PL (1) PL2325332T3 (fr)
PT (1) PT1916903E (fr)
WO (1) WO2007025097A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9512446B1 (en) 2015-08-28 2016-12-06 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
US9567604B2 (en) 2013-03-15 2017-02-14 The General Hospital Corporation Using truncated guide RNAs (tru-gRNAs) to increase specificity for RNA-guided genome editing
US11844760B2 (en) 2015-05-06 2023-12-19 Snipr Technologies Limited Altering microbial populations and modifying microbiota

Families Citing this family (271)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2619833C (fr) 2005-08-26 2017-05-09 Danisco A/S Utilisation de genes associes a crispr-cas
DK2426220T3 (en) * 2006-05-19 2016-09-26 Dupont Nutrition Biosci Aps Labeled microorganisms, and methods for labeling
PL2034848T3 (pl) 2006-06-16 2017-08-31 Dupont Nutrition Bioscences Aps Bakteria Streptococcus thermophilus
AU2013234432B2 (en) * 2007-03-02 2015-09-24 Dupont Nutrition Biosciences Aps Cultures with improved phage resistance
US20110002889A1 (en) 2007-03-02 2011-01-06 Rodolphe Barrangou Cultures with Improved Phage Resistance
WO2010011961A2 (fr) 2008-07-25 2010-01-28 University Of Georgia Research Foundation, Inc. Système procaryote arni-like et ses procédés d'utilisation
WO2010054108A2 (fr) * 2008-11-06 2010-05-14 University Of Georgia Research Foundation, Inc. Polypeptides cas6 et procédés d'utilisation
EP2362915B1 (fr) 2008-11-07 2016-12-21 DuPont Nutrition Biosciences ApS Séquences crispr de bifidobactéries
WO2010067148A1 (fr) * 2008-12-11 2010-06-17 Danisco A/S Compositions bactériennes de staphylococcus vitulinus présentant une activité de nitrate réductase et de bactéries lactiques, et procédés utilisant ces compositions
US20120164275A1 (en) 2009-09-01 2012-06-28 Chr. Hansen A/S Lactic bacterium with modified galactokinase expression for texturizing food products by overexpression of exopolysaccharide
JP5905834B2 (ja) 2010-01-28 2016-04-20 セーホーエル．ハンセン アクティーゼルスカブ ファージ耐性に基づいて選択された食品に質感を加えるための乳酸菌
EP2569425B1 (fr) 2010-05-10 2016-07-06 The Regents of The University of California Compositions d'endoribonucléases et leurs procédés d'utilisation
EP2630156B1 (fr) 2010-10-20 2018-08-22 DuPont Nutrition Biosciences ApS Sequences crispr-cas de lactococcus
EP2630265B1 (fr) 2010-10-22 2017-03-22 Chr. Hansen A/S Souches de bactéries lactiques texturantes
CN102172278B (zh) * 2011-01-21 2015-08-12 吉林紫鑫药物研究有限公司 一种制备果蔬发酵转化液的方法
US20140113376A1 (en) 2011-06-01 2014-04-24 Rotem Sorek Compositions and methods for downregulating prokaryotic genes
WO2013066438A2 (fr) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Évaluation et amélioration de la spécificité de clivage des nucléases
PH12014501360B1 (en) 2011-12-16 2022-05-20 Targetgene Biotechnologies Ltd Compositions and methods for modifying a predetermined target nucleic acid sequence
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
WO2013141680A1 (fr) * 2012-03-20 2013-09-26 Vilnius University Clivage d'adn dirigé par arn par le complexe cas9-arncr
US9637739B2 (en) * 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
LT2800811T (lt) 2012-05-25 2017-09-11 The Regents Of The University Of California Būdai ir kompozicijos, skirti tikslinės dnr modifikavimui, panaudojant adresuotą rnr, ir transkripcijos moduliavimui, panaudojant adresuotą rnr
PT3138912T (pt) 2012-12-06 2018-12-28 Sigma Aldrich Co Llc Modificação e regulação de genoma baseado em crispr
SG11201504523UA (en) 2012-12-12 2015-07-30 Broad Inst Inc Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
ES2786193T3 (es) 2012-12-12 2020-10-09 Broad Inst Inc Modificación por tecnología genética y optimización de sistemas, métodos y composiciones enzimáticas mejorados para la manipulación de secuencias
RU2701662C9 (ru) * 2012-12-12 2019-10-31 Те Брод Инститьют, Инк. Компоненты системы crispr-cas, способы и композиции для манипуляции с последовательностями
ES2701749T3 (es) 2012-12-12 2019-02-25 Broad Inst Inc Métodos, modelos, sistemas y aparatos para identificar secuencias diana para enzimas Cas o sistemas CRISPR-Cas para secuencias diana y transmitir resultados de los mismos
CN105121641A (zh) 2012-12-17 2015-12-02 哈佛大学校长及研究员协会 Rna-引导的人类基因组工程化
AU2014207618A1 (en) 2013-01-16 2015-08-06 Emory University Cas9-nucleic acid complexes and uses related thereto
US11135273B2 (en) 2013-02-07 2021-10-05 The Rockefeller University Sequence specific antimicrobials
WO2014124226A1 (fr) 2013-02-07 2014-08-14 The Rockefeller University Antimicrobiens spécifiques d'une séquence
ES2522765B2 (es) * 2013-02-22 2015-03-18 Universidad De Alicante Método para dectectar inserciones de espaciadores en estructuras CRISPR
US10612043B2 (en) 2013-03-09 2020-04-07 Agilent Technologies, Inc. Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events
UA123532C2 (uk) 2013-03-12 2021-04-21 Е. І. Дю Пон Де Немур Енд Компані Спосіб ідентифікації варіантного сайта розпізнавання для сконструйованого засобу, що рідко розщеплює, для індукції двониткового розриву
US9803214B2 (en) 2013-03-12 2017-10-31 Pioneer Hi-Bred International, Inc. Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility
EP3620534B1 (fr) 2013-03-14 2021-10-13 Caribou Biosciences, Inc. Crispr-cas compositions pour des acides nucléiques à ciblage d'acide nucléique
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
US10760064B2 (en) 2013-03-15 2020-09-01 The General Hospital Corporation RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
CN115261411A (zh) * 2013-04-04 2022-11-01 哈佛学院校长同事会 利用CRISPR/Cas系统的基因组编辑的治疗性用途
US9902973B2 (en) 2013-04-11 2018-02-27 Caribou Biosciences, Inc. Methods of modifying a target nucleic acid with an argonaute
KR20240172759A (ko) 2013-06-17 2024-12-10 더 브로드 인스티튜트, 인코퍼레이티드 간의 표적화 및 치료를 위한 ＣＲＩＳＰＲ­Ｃａｓ 시스템, 벡터 및 조성물의 전달 및 용도
WO2014204727A1 (fr) 2013-06-17 2014-12-24 The Broad Institute Inc. Génomique fonctionnelle utilisant des systèmes crispr-cas, procédés de composition, cribles et applications de ces derniers
ES2777217T3 (es) 2013-06-17 2020-08-04 Broad Inst Inc Suministro, modificación y optimización de sistemas de guía en tándem, métodos y composiciones para la manipulación de secuencias
CA2915795C (fr) 2013-06-17 2021-07-13 The Broad Institute, Inc. Procede de traitement de dechets organiques destine a fournir un engraisa liberation lente
JP6738729B2 (ja) * 2013-06-17 2020-08-12 ザ・ブロード・インスティテュート・インコーポレイテッド 分裂終了細胞の疾患および障害をターゲティングおよびモデリングするための系、方法および組成物の送達、エンジニアリングおよび最適化
AU2014281027A1 (en) 2013-06-17 2016-01-28 Massachusetts Institute Of Technology Optimized CRISPR-Cas double nickase systems, methods and compositions for sequence manipulation
US10011850B2 (en) 2013-06-21 2018-07-03 The General Hospital Corporation Using RNA-guided FokI Nucleases (RFNs) to increase specificity for RNA-Guided Genome Editing
WO2015006498A2 (fr) 2013-07-09 2015-01-15 President And Fellows Of Harvard College Utilisations thérapeutiques d'édition du génome avec des systèmes crispr/cas
CN103388006B (zh) * 2013-07-26 2015-10-28 华东师范大学 一种基因定点突变的构建方法
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
BR112016003561B8 (pt) 2013-08-22 2022-11-01 Du Pont Método para a produção de uma modificação genética, método para a introdução de um polinucleotídeo de interesse no genoma de uma planta, método para a edição de um segundo gene em um genoma de planta e método para gerar uma planta de milho resistente ao glifosato
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
EP3041498B1 (fr) * 2013-09-05 2022-02-16 Massachusetts Institute of Technology Réglage de populations microbiennes à l'aide de nucléases programmables
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US10584358B2 (en) 2013-10-30 2020-03-10 North Carolina State University Compositions and methods related to a type-II CRISPR-Cas system in Lactobacillus buchneri
DK3066201T3 (en) 2013-11-07 2018-06-06 Editas Medicine Inc CRISPR-RELATED PROCEDURES AND COMPOSITIONS WITH LEADING GRADES
RU2016125246A (ru) 2013-11-27 2018-01-09 Е. И. Дюпон Де Немур Энд Компани Генетические локусы, связанные с реакцией на абиотический стресс
WO2015089364A1 (fr) 2013-12-12 2015-06-18 The Broad Institute Inc. Structure cristalline d'un système crispr-cas, et ses utilisations
US20150166985A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting von willebrand factor point mutations
SG10201804975PA (en) 2013-12-12 2018-07-30 Broad Inst Inc Delivery, Use and Therapeutic Applications of the Crispr-Cas Systems and Compositions for HBV and Viral Diseases and Disorders
BR112016013520A2 (pt) 2013-12-12 2017-10-03 Broad Inst Inc Composições e métodos de uso de sistemas crispr-cas em distúrbios de repetições de nucleotídeos
WO2015089486A2 (fr) 2013-12-12 2015-06-18 The Broad Institute Inc. Systèmes, procédés et compositions pour manipulation de séquences avec systèmes crispr-cas fonctionnels optimisés
EP3653229A1 (fr) 2013-12-12 2020-05-20 The Broad Institute, Inc. Distribution, utilisation et applications thérapeutiques des systèmes crispr-cas et compositions pour l'édition du génome
MX375420B (es) 2014-02-11 2025-03-06 The Regents Of The Univ Of Colorado A Body Corporate Ingenieria genetica multiplexada habilitada por crispr.
EP3105325B2 (fr) 2014-02-13 2024-10-23 Takara Bio USA, Inc. Procédés d'appauvrissement d'une molécule cible d'une ensemble initial d'acides nucléiques, et compositions et kits pour la mise en oeuvre de ceux-ci
HUE051354T2 (hu) 2014-04-14 2021-03-01 Nemesis Bioscience Ltd Terapeutikum
AU2015280069B2 (en) 2014-06-23 2021-08-12 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
AU2015288157A1 (en) 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
CA2956224A1 (fr) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Proteines cas9 comprenant des inteines dependant de ligands
US20170218384A1 (en) 2014-08-08 2017-08-03 Pioneer Hi-Bred International, Inc. Ubiquitin promoters and introns and methods of use
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
US11071289B2 (en) 2014-08-14 2021-07-27 Biocytogen Boston Corp DNA knock-in system
US11274317B2 (en) 2014-09-05 2022-03-15 Vilnius University Targeted RNA knockdown and knockout by type III-A Csm complexes
WO2016035044A1 (fr) * 2014-09-05 2016-03-10 Vilnius University Défibrage programmable de l'arn par le système cas-crispr de type iii-a de streptococcus thermophilus
CN108064129A (zh) 2014-09-12 2018-05-22 纳幕尔杜邦公司 玉米和大豆中复合性状基因座的位点特异性整合位点的产生和使用方法
CA2963693A1 (fr) 2014-10-10 2016-04-14 Editas Medicine, Inc. Compositions et procedes pour activer une reparation dirigee par homologie
EP3215623A4 (fr) 2014-11-06 2018-09-26 President and Fellows of Harvard College Cellules ne présentant pas d'expression en surface de b2m et procédés pour l'administration allogène de ces cellules
KR102424721B1 (ko) 2014-11-06 2022-07-25 이 아이 듀폰 디 네모아 앤드 캄파니 Rna-유도 엔도뉴클레아제의 세포 내로의 펩티드 매개성 전달
WO2016073990A2 (fr) 2014-11-07 2016-05-12 Editas Medicine, Inc. Procédés pour améliorer l'édition génomique médiée par crispr/cas
EP3224353B9 (fr) 2014-11-26 2023-08-09 Technology Innovation Momentum Fund (Israel) Limited Partnership Élimination ciblée de gènes bactériens
CN116059378A (zh) 2014-12-10 2023-05-05 明尼苏达大学董事会 用于治疗疾病的遗传修饰的细胞、组织和器官
EP3889260A1 (fr) 2014-12-12 2021-10-06 The Broad Institute, Inc. Arn guides protégés (pgrnas)
WO2016100309A1 (fr) 2014-12-16 2016-06-23 Pioneer Hi-Bred International, Inc. Rétablissement de la fertilité mâle dans le blé
CA2971462A1 (fr) 2015-02-04 2016-08-11 Pioneer Hi-Bred International, Inc. Nouveaux recepteurs de toxine btet procedes d'utilisation
WO2016137774A1 (fr) 2015-02-25 2016-09-01 Pioneer Hi-Bred International Inc Composition et procédés pour la régulation de l'expression d'un complexe arn guide/endonucléase cas
US10202589B2 (en) 2015-03-03 2019-02-12 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases with altered PAM specificity
EP3274460A1 (fr) 2015-03-27 2018-01-31 E. I. du Pont de Nemours and Company Promoteurs de gène d'arn nucléaire de petite taille u6 de soja et leur utilisation dans l'expression constitutive de gènes d'arn de petite taille dans des plantes
WO2016176617A2 (fr) 2015-04-29 2016-11-03 New York University Procédé pour le traitement de gliomes de haut grade
US20180142251A1 (en) 2015-05-06 2018-05-24 Pioneer Hi-Bred International, Inc. Methods and compositions for the production of unreduced, non-recombined gametes and clonal offspring
AU2016261600B2 (en) 2015-05-08 2021-09-23 President And Fellows Of Harvard College Universal donor stem cells and related methods
US11390884B2 (en) 2015-05-11 2022-07-19 Editas Medicine, Inc. Optimized CRISPR/cas9 systems and methods for gene editing in stem cells
CN107849546A (zh) 2015-05-15 2018-03-27 先锋国际良种公司 对cas内切核酸酶系统、pam序列和指导rna元件的快速表征
CA2986262A1 (fr) 2015-06-09 2016-12-15 Editas Medicine, Inc. Procedes lies a crispr/cas et compositions d'amelioration de la transplantation
CN107771218B (zh) 2015-06-17 2021-12-31 先锋国际良种公司 植物调节元件及其使用方法
WO2016205759A1 (fr) 2015-06-18 2016-12-22 The Broad Institute Inc. Modification et optimisation de systèmes, de méthodes, d'enzymes et d'échafaudages guides d'orthologues de cas9 et variant pour la manipulation de séquences
KR102575342B1 (ko) 2015-06-18 2023-09-05 더 브로드 인스티튜트, 인코퍼레이티드 표적외 효과를 감소시키는 crispr 효소 돌연변이
GB2595063B (en) 2015-07-31 2022-03-09 Univ Minnesota Modified cells and methods of therapy
GB201514510D0 (en) 2015-08-14 2015-09-30 Nemesis Bioscience Ltd Delivery vehicle
WO2017040348A1 (fr) 2015-08-28 2017-03-09 The General Hospital Corporation Nucléases crispr-cas9 modifiées
US9926546B2 (en) 2015-08-28 2018-03-27 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
CA3000816A1 (fr) 2015-09-11 2017-03-16 The General Hospital Corporation Interrogation complete de dsb nucleasiques et sequencage (find-seq)
CA2999500A1 (fr) 2015-09-24 2017-03-30 Editas Medicine, Inc. Utilisation d'exonucleases pour ameliorer l'edition de genome a mediation par crispr/cas
US9850484B2 (en) 2015-09-30 2017-12-26 The General Hospital Corporation Comprehensive in vitro reporting of cleavage events by sequencing (Circle-seq)
CA3000917A1 (fr) 2015-10-09 2017-04-13 Monsanto Technology Llc Nucleases guidees par arn et leurs utilisations
KR102628801B1 (ko) 2015-10-12 2024-01-25 이아이디피, 인크. 세포내 유전자 변형 및 증가된 상동 재조합을 위한 보호 dna 주형 및 이용 방법
MX2018004808A (es) 2015-10-20 2018-08-01 Pioneer Hi Bred Int Funcion restauradora para un producto genico no funcional mediante sistemas cas guiados y metodos de uso.
JP7067793B2 (ja) 2015-10-23 2022-05-16 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸塩基編集因子およびその使用
US11330776B2 (en) 2015-10-30 2022-05-17 Pioneer Hi-Bred International, Inc. Methods and compositions for rapid plant transformation
BR112018007796A2 (pt) 2015-11-06 2018-10-30 Du Pont plantas de soja, partes de plantas de soja ou sementes de soja, método para selecionar uma célula de soja, métodos de seleção de uma célula de soja e de produção de um locus e molécula de ácido nucleico
EP3371313B1 (fr) 2015-11-06 2020-10-14 Pioneer Hi-Bred International, Inc. Procédés et compositions de transformation végétale améliorée
CN115786368A (zh) 2015-11-30 2023-03-14 先锋国际良种公司 植物调节元件及其使用方法
US20180362961A1 (en) 2015-12-11 2018-12-20 Danisco Us Inc. Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects
WO2017105991A1 (fr) 2015-12-18 2017-06-22 Danisco Us Inc. Procédés et compositions pour l'expression d'arn de guidage à base d'arnt
EP4257694A3 (fr) 2015-12-22 2023-12-06 Pioneer Hi-Bred International, Inc. Promoteurs préférés par un tissu et procédés d'utilisation
EP3402885A4 (fr) 2016-01-11 2019-07-03 The Board of Trustees of the Leland Stanford Junior University Protéines chimériques et méthodes de régulation de l'expression génique
MY194127A (en) 2016-01-11 2022-11-14 Univ Leland Stanford Junior Chimeric proteins and methods of immunotherapy
WO2017132239A1 (fr) 2016-01-26 2017-08-03 Pioneer Hi-Bred International, Inc. Maïs cireux
DK3408382T3 (da) 2016-01-27 2022-06-20 Oncorus Inc Onkolytiske virale vektorer og anvendelser deraf
EP3699281A1 (fr) 2016-03-11 2020-08-26 Pioneer Hi-Bred International, Inc. Nouveaux systèmes cas9 et procédés d'utilisation
MX2018011298A (es) 2016-03-18 2019-03-07 Pioneer Hi Bred Int Metodos y composiciones para producir gametos clonados, sin reducir, sin recombinar.
EP3433363A1 (fr) 2016-03-25 2019-01-30 Editas Medicine, Inc. Systèmes d'édition de génome comprenant des molécules d'enzyme modulant la réparation et leurs procédés d'utilisation
WO2017180694A1 (fr) 2016-04-13 2017-10-19 Editas Medicine, Inc. Systèmes d'édition de gènes de molécules de fusion cas9 et leurs procédés d'utilisation
JP7184648B2 (ja) 2016-04-29 2022-12-06 ビーエーエスエフ プラント サイエンス カンパニー ゲーエムベーハー 標的核酸の改変のための改善された方法
GB201609811D0 (en) 2016-06-05 2016-07-20 Snipr Technologies Ltd Methods, cells, systems, arrays, RNA and kits
US20200332305A1 (en) 2016-06-14 2020-10-22 Pioneer Hi-Bred International, Inc. Use of cpfi endonuclease for plant genome modifications
US20190100745A1 (en) 2016-06-20 2019-04-04 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
CN109688820B (zh) 2016-06-24 2023-01-10 科罗拉多州立大学董事会（法人团体） 用于生成条形码化组合文库的方法
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
GB2568182A (en) 2016-08-03 2019-05-08 Harvard College Adenosine nucleobase editors and uses thereof
EP3497214B1 (fr) 2016-08-09 2023-06-28 President and Fellows of Harvard College Protéines de fusion cas9-recombinase programmables et utilisations associées
WO2018039438A1 (fr) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation d'acides aminés non naturels dans des protéines au moyen de l'édition de bases
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
BR112019007327A2 (pt) 2016-10-13 2019-07-02 Pioneer Hi Bred Int método para obter uma célula vegetal, célula vegetal, planta, semente, polinucleotídeo-guia
GB2573062A (en) 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
GB2605883B (en) 2016-10-18 2023-03-15 Univ Minnesota Tumor infiltrating lymphocytes and methods of therapy
CN109906030B (zh) 2016-11-04 2022-03-18 安健基因公司 用于产生仅重链抗体的经基因修饰的非人动物和方法
US11236348B2 (en) 2016-11-29 2022-02-01 Pioneer Hi-Bred International, Inc. Transcriptional terminators for gene expression in plants
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
EP4095263A1 (fr) 2017-01-06 2022-11-30 Editas Medicine, Inc. Procédés d'évaluation de la coupure par les nucléases
WO2018129440A1 (fr) 2017-01-09 2018-07-12 University Of Massachusetts Complexes destinés à la délétion et l'édition de gènes
EP3592853A1 (fr) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression de la douleur par édition de gène
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
US11268082B2 (en) 2017-03-23 2022-03-08 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable DNA binding proteins
AU2018254547B2 (en) 2017-04-20 2024-06-13 Egenesis, Inc. Methods for generating genetically modified animals
US11591589B2 (en) 2017-04-21 2023-02-28 The General Hospital Corporation Variants of Cpf1 (Cas12a) with altered PAM specificity
WO2018197520A1 (fr) 2017-04-24 2018-11-01 Dupont Nutrition Biosciences Aps Procédés et compositions de protéines anti-crispr destinés à être utilisés dans des plantes
US11499151B2 (en) 2017-04-28 2022-11-15 Editas Medicine, Inc. Methods and systems for analyzing guide RNA molecules
WO2018209320A1 (fr) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Arn guides incorporés par aptazyme pour une utilisation avec crispr-cas9 dans l'édition du génome et l'activation transcriptionnelle
AU2018272067B2 (en) 2017-05-25 2024-07-18 The General Hospital Corporation Base editors with improved precision and specificity
CN110997908A (zh) 2017-06-09 2020-04-10 爱迪塔斯医药公司 工程化的cas9核酸酶
CN111344020A (zh) 2017-06-15 2020-06-26 加利福尼亚大学董事会 靶向非病毒dna插入
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
CN111511375A (zh) 2017-06-30 2020-08-07 因提玛生物科学公司 用于基因治疗的腺相关病毒载体
US20190002874A1 (en) 2017-06-30 2019-01-03 Inscripta, Inc. Cell libraries created using rationally designed nucleic acids
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
WO2019023483A1 (fr) 2017-07-26 2019-01-31 Oncorus, Inc. Vecteurs viraux oncolytiques et leurs utilisations
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019027861A1 (fr) 2017-07-31 2019-02-07 R. J. Reynolds Tobacco Company Procédés et compositions pour l'édition de gènes à base de virus dans des plantes
NL2019390B1 (en) 2017-08-04 2019-02-21 Univ Leiden Screening Method
CN111263810A (zh) 2017-08-22 2020-06-09 纳匹基因公司 使用多核苷酸指导的核酸内切酶的细胞器基因组修饰
BR112020003596A2 (pt) 2017-08-23 2020-09-01 The General Hospital Corporation nucleases de crispr-cas9 engenheiradas com especificidade de pam alterada
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019139645A2 (fr) 2017-08-30 2019-07-18 President And Fellows Of Harvard College Éditeurs de bases à haut rendement comprenant une gam
EP3688171A1 (fr) 2017-09-25 2020-08-05 Pioneer Hi-Bred International, Inc. Promoteurs ayant une préférence pour des tissus et méthodes d'utilisation
US10435713B2 (en) 2017-09-30 2019-10-08 Inscripta, Inc. Flow through electroporation instrumentation
US11725228B2 (en) 2017-10-11 2023-08-15 The General Hospital Corporation Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies
MX2020004138A (es) 2017-10-13 2020-08-13 Pioneer Hi Bred Int Sistemas y metodos para la reprogramacion celular de una celula vegetal.
EP3697906A1 (fr) 2017-10-16 2020-08-26 The Broad Institute, Inc. Utilisations d'éditeurs de bases adénosine
MX2020004325A (es) 2017-10-27 2020-11-09 Univ California Reemplazo dirigido de receptores de células t endógenos.
WO2019118463A1 (fr) 2017-12-15 2019-06-20 Danisco Us Inc Variants de cas9 et procédés d'utilisation
WO2019133371A1 (fr) 2017-12-27 2019-07-04 Pioneer Hi-Bred International, Inc. Transformation de plantes dicotylédones
US20200362366A1 (en) 2018-01-12 2020-11-19 Basf Se Gene underlying the number of spikelets per spike qtl in wheat on chromosome 7a
KR20200124702A (ko) 2018-02-23 2020-11-03 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 신규한 cas9 오르소로그
US10760075B2 (en) 2018-04-30 2020-09-01 Snipr Biome Aps Treating and preventing microbial infections
CN112204131A (zh) 2018-03-29 2021-01-08 因思科瑞普特公司 用于诱导和转化的细胞生长速率的自动化控制
US10376889B1 (en) 2018-04-13 2019-08-13 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
WO2019204378A1 (fr) 2018-04-17 2019-10-24 The General Hospital Corporation Dosages in vitro sensibles pour des préférences de substrat et de sites d'agents de liaison, de modification et de clivage d'acide nucléique
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10508273B2 (en) 2018-04-24 2019-12-17 Inscripta, Inc. Methods for identifying selective binding pairs
EP3788152A4 (fr) * 2018-05-04 2022-03-09 Locus Biosciences, Inc. Procédés et compositions pour détruire une bactérie cible
CN112654710A (zh) 2018-05-16 2021-04-13 辛瑟高公司 用于指导rna设计和使用的方法和系统
EP3797160A1 (fr) 2018-05-23 2021-03-31 The Broad Institute Inc. Éditeurs de bases et leurs utilisations
CA3097915A1 (fr) 2018-06-28 2020-01-02 Pioneer Hi-Bred International, Inc. Procedes de selection de plantes transformees
WO2020005667A1 (fr) 2018-06-29 2020-01-02 Pioneer Hi-Bred International, Inc. Gène nac édité chez les plantes
WO2020005383A1 (fr) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules et procédés pour une détection améliorée de séquences éditées dans des cellules vivantes
BR112021002792A2 (pt) 2018-08-13 2021-06-29 Pioneer Hi-Bred International, Inc. novos receptores de toxinas inseticidas e métodos de uso
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
EP3844263A4 (fr) 2018-08-30 2022-05-25 Inscripta, Inc. Détection améliorée de séquences à édition par nucléase dans des modules automatisés et des instruments
US11851663B2 (en) 2018-10-14 2023-12-26 Snipr Biome Aps Single-vector type I vectors
EP3866583A1 (fr) 2018-10-16 2021-08-25 Pioneer Hi-Bred International, Inc. Cartographie fine résultant d'une édition du génome et identification de gène causal
WO2020086475A1 (fr) 2018-10-22 2020-04-30 Inscripta, Inc. Enzymes modifiées
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
CA3111090A1 (fr) 2018-10-31 2020-05-07 Pioneer Hi-Bred International, Inc. Compositions et procedes de transformation de plante mediee par ochrobactrum
CA3117228A1 (fr) 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Nouveaux systemes crispr-cas d'edition du genome
WO2020127975A1 (fr) 2018-12-20 2020-06-25 Basf Plant Science Company Gmbh Administration native de biomolécules dans des cellules végétales à l'aide de complexes ioniques avec des peptides de pénétration cellulaire
WO2020163396A1 (fr) 2019-02-04 2020-08-13 The General Hospital Corporation Variants d'éditeur de base d'adn adénine avec édition d'arn hors cible réduite
WO2020185751A1 (fr) 2019-03-11 2020-09-17 Pioneer Hi-Bred International, Inc. Procédés de production de plantes clonales
WO2020191248A1 (fr) 2019-03-19 2020-09-24 The Broad Institute, Inc. Procédés et compositions pour l'édition de séquences nucléotidiques
US11001831B2 (en) 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
AU2020247900A1 (en) 2019-03-25 2021-11-04 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
US20220170033A1 (en) 2019-03-27 2022-06-02 Pioneer Hi-Bred International, Inc. Plant explant transformation
US20220154193A1 (en) 2019-03-28 2022-05-19 Pioneer Hi-Bred International, Inc. Modified agrobacterium strains and use thereof for plant transformation
EP3947662A1 (fr) 2019-04-05 2022-02-09 Danisco US Inc. Procédés d'intégration d'une séquence d'adn donneur dans le génome de bacillus à l'aide de constructions d'adn recombinant linéaire et compositions associées
EP3947656A1 (fr) 2019-04-05 2022-02-09 Danisco US Inc. Procédés d'intégration de polynucléotides dans le génome de bacillus à l'aide de constructions d'adn recombiné double circulaire et compositions correspondantes
US20220240467A1 (en) 2019-04-18 2022-08-04 Pioneer Hi-Bred International, Inc. Embryogenesis factors for cellular reprogramming of a plant cell
CN110129246B (zh) * 2019-04-29 2021-02-19 华南农业大学 一株供体菌、其构建方法与应用以及质粒抑制剂筛选方法
EP3953477A4 (fr) 2019-06-06 2022-06-22 Inscripta, Inc. Curage pour l'édition récursive de cellules guidée par un acide nucléique
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
AU2020297499A1 (en) 2019-06-21 2022-02-03 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
GB201914137D0 (en) 2019-10-01 2019-11-13 Univ Leeds Innovations Ltd Modified Plants
WO2021087273A1 (fr) * 2019-11-01 2021-05-06 The Trustees Of Columbia University In The City Of New York Génération de bibliothèques d'arn crispr à l'échelle du génome à l'aide d'une adaptation de crispr dans des bactéries
CN114981408A (zh) * 2019-11-06 2022-08-30 洛可斯生物科学公司 Crispr cas系统和溶原性模块
WO2021102059A1 (fr) 2019-11-19 2021-05-27 Inscripta, Inc. Procédés pour augmenter l'édition observée dans des bactéries
EP4061939A1 (fr) 2019-11-19 2022-09-28 Danisco US Inc. Procédés sans marqueur de sélection pour modifier le génome de bacillus et compositions de ceux-ci
WO2021105191A1 (fr) 2019-11-29 2021-06-03 Basf Se Augmentation de la résistance contre les infections fongiques chez les plantes
CA3157131A1 (fr) 2019-12-10 2021-06-17 Inscripta, Inc. Nouvelles nucleases mad
US10704033B1 (en) 2019-12-13 2020-07-07 Inscripta, Inc. Nucleic acid-guided nucleases
US20230075913A1 (en) 2019-12-16 2023-03-09 BASF Agricultural Solutions Seed US LLC Codon-optimized cas9 endonuclease encoding polynucleotide
CN114829607A (zh) 2019-12-18 2022-07-29 因思科瑞普特公司 用于体内检测核酸引导的核酸酶编辑的细胞的Cascade/dCas3互补测定
KR20220116504A (ko) 2019-12-19 2022-08-23 바스프 에스이 정밀 화학물의 제조에서 공시 수율, 탄소-전환-효율 및 탄소 기질 적응성의 증가
WO2021122528A1 (fr) 2019-12-20 2021-06-24 Basf Se Diminution de la toxicité de terpènes et augmentation du potentiel de production dans des micro-organismes
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
KR20220133257A (ko) 2020-01-27 2022-10-04 인스크립타 인코포레이티드 전기천공 모듈 및 기구
US20230107598A1 (en) 2020-02-28 2023-04-06 Pioneer Hi-Bred International, Inc. Sorghum doubled haploid production system
WO2021175756A1 (fr) 2020-03-04 2021-09-10 Basf Se Vecteur navette pour l'expression chez e. coli et bacilli
US20230212593A1 (en) 2020-03-04 2023-07-06 Basf Se Method for the production of constitutive bacterial promoters conferring low to medium expression
CN115362370A (zh) 2020-04-02 2022-11-18 先锋国际良种公司 新颖的杀昆虫毒素受体和使用方法
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
EP4146804A1 (fr) 2020-05-08 2023-03-15 The Broad Institute Inc. Méthodes et compositions d'édition simultanée des deux brins d'une séquence nucléotidique double brin cible
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
WO2022060749A1 (fr) 2020-09-15 2022-03-24 Inscripta, Inc. Édition par crispr pour incorporer des plaques d'amarrage d'acide nucléique dans des génomes de cellules vivantes
US20240002870A1 (en) 2020-09-30 2024-01-04 Pioneer Hi-Bred International, Inc. Rapid transformation of monocot leaf explants
EP4231816A1 (fr) 2020-10-21 2023-08-30 Pioneer Hi-Bred International, Inc. Facteurs de parthénogenèse et leurs procédés d'utilisation
CA3197678A1 (fr) 2020-10-21 2022-04-28 Pioneer Hi-Bred International, Inc. Inducteur haploide double
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
AU2021415461A1 (en) 2021-01-04 2023-08-17 Inscripta, Inc. Mad nucleases
US20240376451A1 (en) 2021-01-07 2024-11-14 Inscripta, Inc. Mad nucleases
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
EP4376626A1 (fr) 2021-07-29 2024-06-05 International N&H Denmark ApS Compositions et procédés de production de compositions laitières fermentées ayant un arôme de crème
EP4376627A1 (fr) 2021-07-29 2024-06-05 International N&H Denmark ApS Compositions et procédés de production de compositions végétales fermentées ayant un arôme de crème
EP4384536A1 (fr) * 2021-08-12 2024-06-19 International N&H Denmark ApS Compositions et procédés destinés à réguler l'immunité adaptative dans les bactéries
JP2024537991A (ja) 2021-10-14 2024-10-18 アーセナル バイオサイエンシズ インコーポレイテッド 共発現されるｓｈＲＮＡと論理ゲートシステムとを有する免疫細胞
WO2023082047A1 (fr) 2021-11-09 2023-05-19 Dupont Nutrition Biosciences Aps Compositions et procédés de production de produits laitiers fermentés pour le stockage à température ambiante
WO2023102393A1 (fr) 2021-11-30 2023-06-08 Pioneer Hi-Bred International, Inc. Manipulation de génome chromosomique à grande échelle et à haute efficacité
WO2023107902A1 (fr) 2021-12-06 2023-06-15 Napigen, Inc. Phosphite déshydrogénase en tant que marqueur sélectionnable pour la transformation mitochondriale
US20230279442A1 (en) 2021-12-15 2023-09-07 Versitech Limited Engineered cas9-nucleases and method of use thereof
WO2023109887A1 (fr) 2021-12-15 2023-06-22 南京金斯瑞生物科技有限公司 Procédé de détection de site d'intégration
EP4499841A1 (fr) 2022-03-25 2025-02-05 Pioneer Hi-Bred International, Inc. Procédés d'induction d'haploïdes parthénogénétiques et de doublement de chromosomes haploïdes
GB202207774D0 (en) 2022-05-26 2022-07-13 Cambridge Entpr Ltd Modified plants
GB202209518D0 (en) 2022-06-29 2022-08-10 Snipr Biome Aps Treating & preventing E coli infections
WO2024006802A1 (fr) 2022-06-30 2024-01-04 Pioneer Hi-Bred International, Inc. Procédés et systèmes à médiation par intelligence artificielle pour édition génomique
WO2024036190A2 (fr) 2022-08-09 2024-02-15 Pioneer Hi-Bred International, Inc. Multiplexage de polynucléotides de guidage
GB202214410D0 (en) 2022-09-30 2022-11-16 Ivy Farm Tech Limited genetically modified cells
WO2024118876A1 (fr) 2022-12-01 2024-06-06 Genencor International Bv Ingénierie génomique multiplex itérative dans les cellules microbiennes à l'aide d'un système de marqueurs de sélection auto-exécutable recombiné
WO2024118882A1 (fr) 2022-12-01 2024-06-06 Genencor International Bv Ingénierie génomique multiplex itérative dans les cellules microbiennes à l'aide d'un système de permutation de marqueurs de sélection
WO2024118881A1 (fr) 2022-12-01 2024-06-06 Genencor International Bv Ingénierie itérative du génome multiplex dans les cellules microbiennes à l'aide d'un système de marqueurs de sélection bidirectionnels
WO2024123786A1 (fr) 2022-12-06 2024-06-13 Pioneer Hi-Bred International, Inc. Procédés et compositions pour la co-administration d'adn-t exprimant de multiples polynucléotides guides dans des plantes
GB202219829D0 (en) 2022-12-29 2023-02-15 Ivy Farm Tech Limited Genetically manipulated cells
WO2024196921A1 (fr) 2023-03-20 2024-09-26 Pioneer Hi-Bred International, Inc. Polypeptides cas à reconnaissance de pam modifiée
WO2025027166A1 (fr) 2023-08-01 2025-02-06 Basf Plant Science Company Gmbh Résistance accrue par expression de la protéine msbp1
WO2025027165A1 (fr) 2023-08-01 2025-02-06 Basf Plant Science Company Gmbh Résistance accrue par expression d'une protéine ics
WO2025059184A1 (fr) 2023-09-12 2025-03-20 Pioneer Hi-Bred International, Inc. Procédés et compositions pour générer des haploïdes doublés paternels à génome modifié

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999051771A1 (fr) * 1998-04-03 1999-10-14 Stichting Voor De Technische Wetenschappen Procede de differentiation de souches de bacteries

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2225783A (en) * 1939-04-07 1940-12-24 Lloyd B Jensen Sausage treatment
US3024116A (en) 1959-02-24 1962-03-06 Libby Mcneill And Libby Food processing
US3403032A (en) 1967-10-04 1968-09-24 Agriculture Usa Pure culture fermentation process for pickled cucumbers
US3897307A (en) 1974-10-23 1975-07-29 Hansens Lab Inc Stabilized dry cultures of lactic acid-producing bacteria
US3932674A (en) 1974-11-29 1976-01-13 The United States Of America Controlled bulk vegetable fermentation
US4140800A (en) 1977-06-13 1979-02-20 Leo Kline Freeze-dried natural sour dough starter
US4423079A (en) * 1980-07-14 1983-12-27 Leo Kline Growth promoting compositions for Lactobacillus sanfrancisco and method of preparation
US4621058A (en) 1983-04-11 1986-11-04 Mid-America Dairymen, Inc. Method of preparing cheese starter media
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5097025A (en) 1989-08-01 1992-03-17 The Rockefeller University Plant promoters
NZ239638A (en) * 1990-09-05 1993-12-23 Univ North Carolina State Bacteriophage resistant recombinant bacteria and their use in
JP3631252B2 (ja) * 1994-05-16 2005-03-23 オランダ国 直列変異反復配列オリゴタイピングによる結核菌群細菌の検出および鑑別
FR2738015B1 (fr) 1995-08-22 1997-11-14 Systems Bio Ind Sequences d'adn et plasmides comprenant au moins un mecanisme de resistance aux phases, bacteries les contenant et leur utilisation
US6409175B1 (en) 1999-07-13 2002-06-25 Grant Prideco, Inc. Expandable joint connector
AU6427300A (en) 1999-08-19 2001-03-19 Chr. Hansen A/S Phage resistant lactic acid bacterial mutants
US6849256B1 (en) * 1999-11-08 2005-02-01 Ganeden Biotech Incorporated Inhibition of pathogens by probiotic bacteria
JP2005500847A (ja) 2001-08-24 2005-01-13 アドバンスド セル テクノロジー、インコーポレイテッド 分化誘導薬の同定のためのスクリーニング検定及び細胞治療用の分化細胞の調製
CA2496630A1 (fr) * 2002-08-30 2004-03-11 North Carolina State University Gene resistant a l'infection bacteriophage abiz
EP2287311A1 (fr) * 2003-03-04 2011-02-23 Intercell AG Antigènes pyogènes streptococcus
CA2619833C (fr) 2005-08-26 2017-05-09 Danisco A/S Utilisation de genes associes a crispr-cas
EP2362915B1 (fr) 2008-11-07 2016-12-21 DuPont Nutrition Biosciences ApS Séquences crispr de bifidobactéries
EP2630156B1 (fr) 2010-10-20 2018-08-22 DuPont Nutrition Biosciences ApS Sequences crispr-cas de lactococcus
TWI583376B (zh) 2013-03-20 2017-05-21 Hui-Ling Zeng Compounds isolated from Antelroxicus and their use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999051771A1 (fr) * 1998-04-03 1999-10-14 Stichting Voor De Technische Wetenschappen Procede de differentiation de souches de bacteries

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
BARRANGOU RODOLPHE ET AL: "CRISPR provides acquired resistance against viruses in prokaryotes.", SCIENCE (NEW YORK, N.Y.) 23 MAR 2007, vol. 315, no. 5819, 23 March 2007 (2007-03-23), pages 1709 - 1712, XP002428071, ISSN: 1095-9203 *
BOLOTIN ALEXANDER ET AL: "Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin.", MICROBIOLOGY (READING, ENGLAND) AUG 2005, vol. 151, no. Pt 8, August 2005 (2005-08-01), pages 2551 - 2561, XP002424872, ISSN: 1350-0872 *
BOUCHER I ET AL: "DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms", JOURNAL OF DAIRY SCIENCE, vol. 84, no. 7, July 2001 (2001-07-01), pages 1610 - 1620, XP002424873, ISSN: 0022-0302 *
CLUZEL P-J ET AL: "PHAGE ABORTIVE INFECTION MECHANISM FROM LACTOCOCCUS-LACTIS-SSP-LACTIS EXPRESSION OF WHICH IS MEDIATED BY AN ISO-ISS1 ELEMENT", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 57, no. 12, 1991, pages 3547 - 3551, XP002424874, ISSN: 0099-2240 *
COFFEY AIDAN ET AL: "Bacteriophage-resistance systems in dairy starter strains: Molecular analysis to application", ANTONIE VAN LEEUWENHOEK, vol. 82, no. 1-2, August 2002 (2002-08-01), pages 303 - 321, XP002424878, ISSN: 0003-6072 *
FRANCISCO J M MOJICA ET AL: "Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements", JOURNAL OF MOLECULAR EVOLUTION, SPRINGER-VERLAG, NE, vol. 60, no. 2, 1 February 2005 (2005-02-01), pages 174 - 182, XP019363173, ISSN: 1432-1432 *
GARVEY P ET AL: "CLONING AND DNA SEQUENCE ANALYSIS OF TWO ABORTIVE INFECTION PHAGE RESISTANCE DETERMINANTS FROM THE LACTOCOCCAL PLASMID PNP40", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 61, no. 12, December 1995 (1995-12-01), pages 4321 - 4328, XP000917636, ISSN: 0099-2240 *
JANSEN RUUD ET AL: "Identification of genes that are associated with DNA repeats in prokaryotes", MOLECULAR MICROBIOLOGY, vol. 43, no. 6, March 2002 (2002-03-01), pages 1565 - 1575, XP002424877, ISSN: 0950-382X *
MCGRATH STEPHEN ET AL: "Molecular characterization of a phage-encoded resistance system in Lactococcus lactis", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 5, May 1999 (1999-05-01), pages 1891 - 1899, XP002424875, ISSN: 0099-2240 *
MOJICA F J M ET AL: "Long stretches of short tandem repeats are present in the largest replicons of the Archaea Haloferax mediterranei and Haloferx volcanii and could be involved in replicon partitioning", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 17, no. 1, 1995, pages 85 - 93, XP002091623, ISSN: 0950-382X *
MOJICA FRANCISCO J M ET AL: "Biological significance of a family of regularly spaced repeats in the genomes of Archaea, Bacteria and mitochondria", MOLECULAR MICROBIOLOGY, vol. 36, no. 1, April 2000 (2000-04-01), pages 244 - 246, XP002424876, ISSN: 0950-382X *
O'CONNOR L ET AL: "AbiG, a genotipically novel abortive infection mechanism encoded by plamid pCI750 of Lactococcus lactis subsp. cremoris UC653", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 62, no. 9, September 1996 (1996-09-01), pages 3075 - 3082, XP002114301, ISSN: 0099-2240 *
VISCARDI M ET AL: "Selection of bacteriophage-resistant mutants of Streptococcus thermophilus.", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 55, no. 1, October 2003 (2003-10-01), pages 109 - 119, XP002428070, ISSN: 0167-7012 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9567604B2 (en) 2013-03-15 2017-02-14 The General Hospital Corporation Using truncated guide RNAs (tru-gRNAs) to increase specificity for RNA-guided genome editing
US9567603B2 (en) 2013-03-15 2017-02-14 The General Hospital Corporation Using RNA-guided FokI nucleases (RFNs) to increase specificity for RNA-guided genome editing
US11844760B2 (en) 2015-05-06 2023-12-19 Snipr Technologies Limited Altering microbial populations and modifying microbiota
US9512446B1 (en) 2015-08-28 2016-12-06 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases

Also Published As

Publication number Publication date
CA2619833C (fr) 2017-05-09
EP1916903A2 (fr) 2008-05-07
NZ597299A (en) 2013-03-28
NZ565563A (en) 2012-02-24
DE602006015484D1 (de) 2010-08-26
EP2341149A1 (fr) 2011-07-06
CA2619833A1 (fr) 2007-03-01
US20100093617A1 (en) 2010-04-15
EP1916903B1 (fr) 2010-07-14
ES2398918T3 (es) 2013-03-22
DK2336362T3 (en) 2019-01-21
EP3284833B1 (fr) 2021-12-01
AU2006282983B2 (en) 2012-08-02
WO2007025097A2 (fr) 2007-03-01
EP2341149B1 (fr) 2016-11-23
AU2006282983A1 (en) 2007-03-01
US20140199767A1 (en) 2014-07-17
EP2336362A1 (fr) 2011-06-22
DK2325332T3 (da) 2013-01-28
US10640778B2 (en) 2020-05-05
EP2325332A1 (fr) 2011-05-25
DK3284833T3 (da) 2022-02-07
EP3284833A2 (fr) 2018-02-21
PL2325332T3 (pl) 2013-03-29
CA2961849A1 (fr) 2007-03-01
EP3284833A3 (fr) 2018-06-06
DK1916903T3 (da) 2010-10-25
US9879269B2 (en) 2018-01-30
US10066233B2 (en) 2018-09-04
PT1916903E (pt) 2010-09-03
ATE473637T1 (de) 2010-07-15
DK2341149T3 (en) 2017-02-27
US20170037416A1 (en) 2017-02-09
CA2961849C (fr) 2022-08-02
EP2325332B1 (fr) 2012-10-31
US20130011828A1 (en) 2013-01-10
EP2336362B1 (fr) 2018-09-19

Similar Documents

Publication Publication Date Title
AU2017224999B2 (en) Use
WO2007025097A3 (fr) Utilisation
EP4397759A3 (fr) Composition pour le clivage d'un adn cible comprenant un arn guide spécifique de l'adn cible et un acide nucléique codant pour la protéine cas ou la protéine cas, et leur utilisation
WO2009037473A3 (fr) Caractérisation de nucléobases
WO2011072246A3 (fr) Modification de l'adn induite par l'effecteur tal
EP2129797B8 (fr) Détection d'acide nucléique
EP2431469A3 (fr) Procédés de régulation de l'expression des gènes ou produits génétiques utilisant des composés tétracyclines substitués
EP2567964A3 (fr) Compositions de Monoméres Permettant la Synthése D'Arn, Procédés de Synthése et Procédés de Déprotection
WO2009108217A3 (fr) Compositions comprenant un arnsi de k-ras et procédés d’utilisation
WO2006135684A3 (fr) Methodes et kits pour la synthese d'arn sens
WO2009039189A3 (fr) Compositions comprenant des arnsi de stat3 et leurs procédés d'utilisation
WO2008067423A3 (fr) Procédé d'amélioration de l'introduction d'adn dans des cellules bactériennes
WO2006031800A3 (fr) Transfection inverse de reseaux de cellules pour des analyses structurelles et fonctionnelles de proteines
WO2009019007A3 (fr) Acides nucléiques se liant à sdf-1 et leur utilisation
WO2007014077A3 (fr) Modulation de l'arni du gene rho-a et utilisations de celle-ci
EP1941058A4 (fr) Amplification d'acides nucléiques au moyen d'amorces non aléatoires
EP2128273A4 (fr) Jeu d'amorces pour amplification génique, réactif d'amplification génique le contenant et son utilisation
WO2008016906A3 (fr) Analogues de nucléotides
WO2008029414A3 (fr) Utilisation d'un inhibiteur du facteur de transcription dans la fabrication d'un médicament
WO2010058393A3 (fr) Compositions et procédés pour le pronostic du cancer du côlon
WO2009079606A3 (fr) Cellules de type es induites par microarn et leurs utilisations
WO2009143277A3 (fr) Compositions comprenant un arnsi hscn9a et leurs procédés d'utilisation
WO2009152387A3 (fr) Compositions comprenant de l'arnsi de c-myc et leurs procédés d'utilisation
EP2055774A4 (fr) Jeu d'amorces pour l'amplification du gène sult1a1, réactif pour l'amplification du gène sult1a1 comprenant ledit jeu d'amorces et utilisation du réactif
WO2009039300A8 (fr) Compositions comprenant du siarn hif-1 alpha et procédés pour les utiliser

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2006282983

Country of ref document: AU

Ref document number: 565563

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 2006802307

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2619833

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2006282983

Country of ref document: AU

Date of ref document: 20060825

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 11990885

Country of ref document: US