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CA2619833C - Utilisation de genes associes a crispr-cas - Google Patents

Utilisation de genes associes a crispr-cas Download PDF

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Publication number
CA2619833C
CA2619833C CA2619833A CA2619833A CA2619833C CA 2619833 C CA2619833 C CA 2619833C CA 2619833 A CA2619833 A CA 2619833A CA 2619833 A CA2619833 A CA 2619833A CA 2619833 C CA2619833 C CA 2619833C
Authority
CA
Canada
Prior art keywords
crispr
cell
spacer
repeats
cas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2619833A
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English (en)
Other versions
CA2619833A1 (fr
Inventor
Philippe Horvath
Rodolphe Barrangou
Christophe Fremaux
Patrick Boyaval
Dennis Romero
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International N&H Denmark ApS
Original Assignee
DuPont Nutrition Biosciences ApS
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37692491&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2619833(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by DuPont Nutrition Biosciences ApS filed Critical DuPont Nutrition Biosciences ApS
Priority to CA2961849A priority Critical patent/CA2961849C/fr
Publication of CA2619833A1 publication Critical patent/CA2619833A1/fr
Application granted granted Critical
Publication of CA2619833C publication Critical patent/CA2619833C/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Animal Husbandry (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Communicable Diseases (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne l'utilisation d'un ou de plusieurs gènes cas pour moduler la résistance à l'intérieur d'une cellule vis-à-vis d'un acide nucléique cible ou d'un produit de transcription de celui-ci.
CA2619833A 2005-08-26 2006-08-25 Utilisation de genes associes a crispr-cas Active CA2619833C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2961849A CA2961849C (fr) 2005-08-26 2006-08-25 Utilisation de genes associes a crispr-cas

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US71139605P 2005-08-26 2005-08-26
US60/711,396 2005-08-26
US74768306P 2006-05-19 2006-05-19
US60/747,683 2006-05-19
PCT/US2006/033167 WO2007025097A2 (fr) 2005-08-26 2006-08-25 Utilisation

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2961849A Division CA2961849C (fr) 2005-08-26 2006-08-25 Utilisation de genes associes a crispr-cas

Publications (2)

Publication Number Publication Date
CA2619833A1 CA2619833A1 (fr) 2007-03-01
CA2619833C true CA2619833C (fr) 2017-05-09

Family

ID=37692491

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2619833A Active CA2619833C (fr) 2005-08-26 2006-08-25 Utilisation de genes associes a crispr-cas
CA2961849A Active CA2961849C (fr) 2005-08-26 2006-08-25 Utilisation de genes associes a crispr-cas

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2961849A Active CA2961849C (fr) 2005-08-26 2006-08-25 Utilisation de genes associes a crispr-cas

Country Status (12)

Country Link
US (4) US10066233B2 (fr)
EP (5) EP1916903B1 (fr)
AT (1) ATE473637T1 (fr)
AU (1) AU2006282983B2 (fr)
CA (2) CA2619833C (fr)
DE (1) DE602006015484D1 (fr)
DK (5) DK2341149T3 (fr)
ES (1) ES2398918T3 (fr)
NZ (2) NZ565563A (fr)
PL (1) PL2325332T3 (fr)
PT (1) PT1916903E (fr)
WO (1) WO2007025097A2 (fr)

Families Citing this family (274)

* Cited by examiner, † Cited by third party
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DE602006015484D1 (de) 2010-08-26
EP2341149A1 (fr) 2011-07-06
CA2619833A1 (fr) 2007-03-01
US20100093617A1 (en) 2010-04-15
EP1916903B1 (fr) 2010-07-14
ES2398918T3 (es) 2013-03-22
DK2336362T3 (en) 2019-01-21
EP3284833B1 (fr) 2021-12-01
AU2006282983B2 (en) 2012-08-02
WO2007025097A2 (fr) 2007-03-01
EP2341149B1 (fr) 2016-11-23
AU2006282983A1 (en) 2007-03-01
US20140199767A1 (en) 2014-07-17
EP2336362A1 (fr) 2011-06-22
DK2325332T3 (da) 2013-01-28
US10640778B2 (en) 2020-05-05
EP2325332A1 (fr) 2011-05-25
DK3284833T3 (da) 2022-02-07
EP3284833A2 (fr) 2018-02-21
PL2325332T3 (pl) 2013-03-29
CA2961849A1 (fr) 2007-03-01
EP3284833A3 (fr) 2018-06-06
DK1916903T3 (da) 2010-10-25
US9879269B2 (en) 2018-01-30
US10066233B2 (en) 2018-09-04
PT1916903E (pt) 2010-09-03
WO2007025097A3 (fr) 2007-07-12
ATE473637T1 (de) 2010-07-15
DK2341149T3 (en) 2017-02-27
US20170037416A1 (en) 2017-02-09
CA2961849C (fr) 2022-08-02
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US20130011828A1 (en) 2013-01-10
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