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WO1998021320A3 - Tissue type plasminogen activator (t-pa) variants: compositions and methods of use - Google Patents

Tissue type plasminogen activator (t-pa) variants: compositions and methods of use Download PDF

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Publication number
WO1998021320A3
WO1998021320A3 PCT/US1997/020226 US9720226W WO9821320A3 WO 1998021320 A3 WO1998021320 A3 WO 1998021320A3 US 9720226 W US9720226 W US 9720226W WO 9821320 A3 WO9821320 A3 WO 9821320A3
Authority
WO
WIPO (PCT)
Prior art keywords
variants
compositions
methods
plasminogen activator
tissue type
Prior art date
Application number
PCT/US1997/020226
Other languages
French (fr)
Other versions
WO1998021320A9 (en
WO1998021320A2 (en
Inventor
Edwin L Madison
Original Assignee
Scripps Research Inst
Edwin L Madison
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst, Edwin L Madison filed Critical Scripps Research Inst
Priority to AU55850/98A priority Critical patent/AU735519B2/en
Priority to US09/600,985 priority patent/US6706504B1/en
Priority to CA002271697A priority patent/CA2271697C/en
Priority to JP52266198A priority patent/JP2001505765A/en
Priority to EP97952176A priority patent/EP0950095A2/en
Publication of WO1998021320A2 publication Critical patent/WO1998021320A2/en
Publication of WO1998021320A3 publication Critical patent/WO1998021320A3/en
Publication of WO1998021320A9 publication Critical patent/WO1998021320A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Variants of tissue plasminogen factor exhibit significantly enhanced fibrin stimulation, dramatically increased discrimination among fibrin co-factors, marked resistance to inhibition by PAI-1, and substantially increased zymogenicity, a combination of properties that enhance the therapeutic utility of the enzyme.
PCT/US1997/020226 1996-11-12 1997-11-12 TISSUE TYPE PLASMINOGEN ACTIVATOR (t-PA) VARIANTS: COMPOSITIONS AND METHODS OF USE WO1998021320A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU55850/98A AU735519B2 (en) 1996-11-12 1997-11-12 Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
US09/600,985 US6706504B1 (en) 1996-11-12 1997-11-12 Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
CA002271697A CA2271697C (en) 1996-11-12 1997-11-12 Tissue type plasminogen activator (t-pa) variants: compositions and methods of use
JP52266198A JP2001505765A (en) 1996-11-12 1997-11-12 Variants of tissue-type plasminogen activator (t-PA): compositions and methods of use
EP97952176A EP0950095A2 (en) 1996-11-12 1997-11-12 TISSUE TYPE PLASMINOGEN ACTIVATOR (t-PA) VARIANTS: COMPOSITIONS AND METHODS OF USE

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3065596P 1996-11-12 1996-11-12
US60/030,655 1996-11-12

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09/600,985 A-371-Of-International US6706504B1 (en) 1996-11-12 1997-11-12 Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
US09600985 A-371-Of-International 1997-11-12
US10/705,633 Division US6869778B2 (en) 1996-11-12 2003-11-10 Tissue type plasminogen activator (t-PA) variants: compositions and methods of use

Publications (3)

Publication Number Publication Date
WO1998021320A2 WO1998021320A2 (en) 1998-05-22
WO1998021320A3 true WO1998021320A3 (en) 1998-07-09
WO1998021320A9 WO1998021320A9 (en) 1998-08-27

Family

ID=21855292

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/020226 WO1998021320A2 (en) 1996-11-12 1997-11-12 TISSUE TYPE PLASMINOGEN ACTIVATOR (t-PA) VARIANTS: COMPOSITIONS AND METHODS OF USE

Country Status (6)

Country Link
EP (1) EP0950095A2 (en)
JP (1) JP2001505765A (en)
CN (1) CN1244894A (en)
AU (1) AU735519B2 (en)
CA (1) CA2271697C (en)
WO (1) WO1998021320A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7105333B2 (en) 2001-03-27 2006-09-12 Deadreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 9, the encoded polypeptides and methods based thereon
US7112430B2 (en) 2001-05-14 2006-09-26 Dendreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 10, the encoded polypeptides and methods based thereon
US7125703B2 (en) 2001-03-13 2006-10-24 Dendreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 7, the encoded polypeptides and methods based thereon
US7172892B2 (en) 2001-03-22 2007-02-06 Dendreon Corporation Nucleic acid molecules encoding serine protease CVSP14, the encoded polypeptides and methods based thereon

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999009184A1 (en) * 1997-08-13 1999-02-25 Roche Diagnostics Gmbh Plasminogen activator with enhanced zymogenic power and reduced fibrin linking
US10160813B2 (en) * 2013-01-22 2018-12-25 University Of Tennessee Research Foundation Tissue plasminogen activator antibodies and methods of use
CN114736948B (en) * 2022-06-10 2022-11-08 深圳市帝迈生物技术有限公司 Alpha 2-antifibrinolytic enzyme activity determination kit

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0199574A2 (en) * 1985-04-22 1986-10-29 Genentech, Inc. Human tissue plasminogen activator mutants, methods and intermediates therefor, and compositions using such mutants
WO1990002798A1 (en) * 1988-09-02 1990-03-22 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0199574A2 (en) * 1985-04-22 1986-10-29 Genentech, Inc. Human tissue plasminogen activator mutants, methods and intermediates therefor, and compositions using such mutants
WO1990002798A1 (en) * 1988-09-02 1990-03-22 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PETERSEN L C ET AL: "QUENCHING OF THE AMIDOLYTIC ACTIVITY OF ONE-CHAIN TISSUE-TYPE PLASMINOGEN ACTIVATOR BY MUTATION OF LYSINE-416", BIOCHEMISTRY, vol. 29, no. 14, 1990, WASHINGTON US, pages 3451 - 3457, XP002053064 *
TACHIAS K ET AL: "Variants of tissue-type plasminogen activator which display substantially enhanced stimulation by fibrin", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 31, 4 August 1995 (1995-08-04), BALTIMORE US, pages 18319 - 18322, XP002063362 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7125703B2 (en) 2001-03-13 2006-10-24 Dendreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 7, the encoded polypeptides and methods based thereon
US7172892B2 (en) 2001-03-22 2007-02-06 Dendreon Corporation Nucleic acid molecules encoding serine protease CVSP14, the encoded polypeptides and methods based thereon
US7105333B2 (en) 2001-03-27 2006-09-12 Deadreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 9, the encoded polypeptides and methods based thereon
US7112430B2 (en) 2001-05-14 2006-09-26 Dendreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 10, the encoded polypeptides and methods based thereon

Also Published As

Publication number Publication date
AU735519B2 (en) 2001-07-12
CA2271697C (en) 2007-10-23
AU5585098A (en) 1998-06-03
CN1244894A (en) 2000-02-16
EP0950095A2 (en) 1999-10-20
CA2271697A1 (en) 1998-05-22
WO1998021320A2 (en) 1998-05-22
JP2001505765A (en) 2001-05-08

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