[go: up one dir, main page]

CA2271697A1 - Tissue type plasminogen activator (t-pa) variants: compositions and methods of use - Google Patents

Tissue type plasminogen activator (t-pa) variants: compositions and methods of use Download PDF

Info

Publication number
CA2271697A1
CA2271697A1 CA002271697A CA2271697A CA2271697A1 CA 2271697 A1 CA2271697 A1 CA 2271697A1 CA 002271697 A CA002271697 A CA 002271697A CA 2271697 A CA2271697 A CA 2271697A CA 2271697 A1 CA2271697 A1 CA 2271697A1
Authority
CA
Canada
Prior art keywords
variants
compositions
methods
plasminogen activator
tissue type
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002271697A
Other languages
French (fr)
Other versions
CA2271697C (en
Inventor
Edwin L. Madison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2271697A1 publication Critical patent/CA2271697A1/en
Application granted granted Critical
Publication of CA2271697C publication Critical patent/CA2271697C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Variants of tissue plasminogen factor exhibit significantly enhanced fibrin stimulation, dramatically increased discrimination among fibrin co-factors, marked resistance to inhibition by PAI-1, and substantially increased zymogenicity, a combination of properties that enhance the therapeutic utility of the enzyme.
CA002271697A 1996-11-12 1997-11-12 Tissue type plasminogen activator (t-pa) variants: compositions and methods of use Expired - Fee Related CA2271697C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3065596P 1996-11-12 1996-11-12
US60/030,655 1996-11-12
PCT/US1997/020226 WO1998021320A2 (en) 1996-11-12 1997-11-12 TISSUE TYPE PLASMINOGEN ACTIVATOR (t-PA) VARIANTS: COMPOSITIONS AND METHODS OF USE

Publications (2)

Publication Number Publication Date
CA2271697A1 true CA2271697A1 (en) 1998-05-22
CA2271697C CA2271697C (en) 2007-10-23

Family

ID=21855292

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002271697A Expired - Fee Related CA2271697C (en) 1996-11-12 1997-11-12 Tissue type plasminogen activator (t-pa) variants: compositions and methods of use

Country Status (6)

Country Link
EP (1) EP0950095A2 (en)
JP (1) JP2001505765A (en)
CN (1) CN1244894A (en)
AU (1) AU735519B2 (en)
CA (1) CA2271697C (en)
WO (1) WO1998021320A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8864398A (en) * 1997-08-13 1999-03-08 Boehringer Mannheim Gmbh Plasminogen activator with enhanced zymogenic power and reduced fibrin linking
US7125703B2 (en) 2001-03-13 2006-10-24 Dendreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 7, the encoded polypeptides and methods based thereon
JP2004535166A (en) 2001-03-22 2004-11-25 デンドレオン・サンディエゴ・リミテッド・ライアビリティ・カンパニー Nucleic acid molecule encoding serine protease CVSP14, encoded polypeptide and methods based thereon
US7105333B2 (en) 2001-03-27 2006-09-12 Deadreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 9, the encoded polypeptides and methods based thereon
KR20040080940A (en) 2001-05-14 2004-09-20 덴드레온 코포레이션 Nucleic acid molecules encoding a transmembrane serine protease 10, the encoded polypeptides and methods based thereon
EP2948167A4 (en) * 2013-01-22 2016-10-19 Univ Tennessee Res Foundation TISSUE PLASMINOGEN ACTIVATOR ANTIBODIES AND METHODS OF USE
CN114736948B (en) * 2022-06-10 2022-11-08 深圳市帝迈生物技术有限公司 Alpha 2-antifibrinolytic enzyme activity determination kit

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE68825T1 (en) * 1985-04-22 1991-11-15 Genentech Inc HUMAN TISSUE PLASMINOGEN ACTIVATOR MUTANT, PROCESSES AND INTERMEDIATE PRODUCTS THEREOF, AND COMPOSITIONS USING SUCH MUTANT.
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties

Also Published As

Publication number Publication date
WO1998021320A3 (en) 1998-07-09
CA2271697C (en) 2007-10-23
CN1244894A (en) 2000-02-16
WO1998021320A2 (en) 1998-05-22
AU5585098A (en) 1998-06-03
EP0950095A2 (en) 1999-10-20
JP2001505765A (en) 2001-05-08
AU735519B2 (en) 2001-07-12

Similar Documents

Publication Publication Date Title
CA2295693A1 (en) Mutant .alpha.-amylase having introduced therein a disulfide bond
AU677540B2 (en) High-strength line-pipe steel having low yield ratio and excellent low-temperature toughness
AU3645697A (en) Amidinoindoles, amidinoazoles, and analogs thereof as inhibitors of factor xa and of thrombin
CA2321263A1 (en) Use of bacteria endowed with arginine deiminase to induce apoptosis and/or reduce an inflammatory reaction and pharmaceutical or dietetic compositions containing such bacteria
AU1453200A (en) Cyclic peptide ligands that target urokinase plasminogen activator receptor, their pharmaceutical compositions and use
EP0278696A3 (en) An arg-serpin human plasminogen activator inhibitor designated pai-2
AU5186799A (en) Peptides of il-2 and derivatives thereof and their use as therapeutic agents
AU1515295A (en) Inhibitors of urokinase plasminogen activator
WO1999006387A3 (en) O-substituted hydroxycumaranone derivatives as antitumor and antimetastatic agents
UA66793C2 (en) Leptin as inhibitor of tumor growth
EP1167354A3 (en) Racemic huperzine A
AU6806487A (en) Tissue plasminogen activator
CA2330972A1 (en) Fully recombinant tissue sealant compositions
CA2271697A1 (en) Tissue type plasminogen activator (t-pa) variants: compositions and methods of use
EP1825864A3 (en) Antithrombotic agents
AU3951995A (en) Urokinase plasminogen activator fragments
AU7431796A (en) Brain-associated inhibitor of tissue-type plasminogen activator
AU2530199A (en) Targeting immunoreagents useful in therapeutic and diagnostic compositions and methods
GB9515279D0 (en) Improved therapeutic agents
CA2283450A1 (en) Methods for treatment of scar tissue
CA2136841A1 (en) Novel Piperidyl Amides, Sulfonamides and Sulfoxamides as Inhibitors of Cholesterol Biosynthesis
AU3630695A (en) Semi-synthetic lipopeptides, compositions containing said lipopeptides, and methods of use
AU628491B2 (en) Mutants of urinary plasminogen activator, their production and use
AP2000001950A0 (en) Paroxetine maleate.
AU7719194A (en) Machine dishwashing composition comprising lipolytic and proteolytic enzymes

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed