UA122778U - METHOD OF OBTAINING SUBSTANCE WITH HYPOGLYCEMIC ACTION FROM ROOTE FLOWERS OF YACON - Google Patents

Abstract

Method of obtaining substance with hypoglycemic action by extraction of vegetable raw material with hot water, followed by filtration, evaporation and drying. As vegetable raw materials use root tubers of anchors, extraction is carried out at a ratio of raw materials: extractant 1: 8.

Description

The useful model belongs to pharmacy and medicine, namely to methods of obtaining biologically active substances from plant raw materials, in particular, hypoglycemic substances from yacon root tubers, which affect the blood glucose content in the treatment of diseases associated with carbohydrate metabolism disorders, such as diabetes.

Plants that are a source of phenolic compounds, polysaccharides-fructans, including inulin, include yacon (Roiutpia vopspiioia Roerr. 4 Epaim. - a synonym of Ztaiiapiiv vopspioiia) - a perennial plant from the Aviegaseae family. Biologically active substances of yacon have the ability to reduce blood sugar, improve metabolic processes in the body, influence the function of the gastrointestinal tract, and have antisclerotic effects (1-3).

There is a known method of obtaining a complex of biologically active compounds, which exhibits hypoglycemic activity by extracting the golden bean herb with 50 95 ethanol, with a ratio of raw materials: extractant of 1:8-1:10, infusion at room temperature for 24 hours, followed by percolation, evaporation to an aqueous residue , sedimentation, separation of the precipitate of resinous substances, precipitation of proteins and polysaccharides with a double volume of 9695 ethanol and evaporation of the filtrate to an aqueous residue. The aqueous residue is treated with five times equal volumes of methylene chloride, and the purified aqueous extract is evaporated to a dry residue. The output of the final product is 5.4 95 from air-dry raw materials

AND.

Disadvantages of this method include long duration, cleaning of the extract with an organochlorine solvent, low yield of the finished product, and its hygroscopicity.

The basis of a useful model is the task of improving a known method in which, by changing the technological method, aimed at maximally complete extraction of extractive and biologically active substances from plant raw materials, which will allow to obtain not only a qualitatively new medicinal substance with a high content of biologically active substances, but also to expand an assortment of herbal medicines with hypoglycemic effect from yacon root tubers.

The task is solved in such a way that in this method of obtaining a complex of biologically active substances from medicinal plant raw materials, which includes extraction with hot water, purified, pre-treated with 9695 ethanol plant raw materials, as well as,

Further evaporation to a thick extract leads to obtaining a medicinal substance with a high content of biologically active substances.

The choice of the extractant and parameters of the method is determined experimentally, taking into account the degree of biological activity of obtaining the final product, its effectiveness, availability and safety when obtained in industrial conditions.

It was experimentally established that the use of purified hot water as an extractant under the given conditions ensures the most complete extraction of biologically active substances in the final product. It is worth noting that pre-soaking of vegetable raw materials in 96 9% ethanol ensures the release of the extract from ballast substances and excessive mucus content.

In the course of a complex phytochemical study of yacon root tubers conducted by us, the qualitative composition and quantitative content of biologically active substances in the studied object was established: carbohydrates (in particular inulin), amino acids, organic acids, macro- and microelements, a complex of phenolic compounds (flavonoids, hydroxycinnamic acids ). The listed complex of biologically active substances exerts a significant influence on carbohydrate metabolism in the human body.

Based on the above, the task is solved in such a way that in the known method of obtaining a plant substance with a hypoglycemic effect, which includes a technological step of extraction, according to a useful model, as a plant raw material, the root tubers of yacon (Roiutpia zopspyoiia Roerr. is Epaii) are used, which are extracted with purified water (temperature - 80-90 C) in a ratio of 1:8, and the crushed plant material is pre-treated with 96-95% ethanol, then the meal is filtered without prior cooling, and the resulting extract is thickened to obtain a thick extract (loss in mass during drying was 13.77 Ob).

Example 1 100 g of air-dried yacon root tubers (Roiutpia zopsioia Roerr. 5 Epai) crushed to the size of particles that pass through a sieve with a hole diameter of 11200 were soaked in 96 95 ethanol and left for 15 minutes. at room temperature to degrease raw materials. Then the processed raw materials were transferred to a percolator and poured with 800 ml of hot purified water (i - 80-9070), infused for 24 hours. at room temperature with occasional stirring. The resulting aqueous extract was filtered through a paper filter and condensed in a rotary vacuum evaporator at a temperature of 50-60 "C until a thick extract was obtained (mass loss during drying was 13.77 95).

The obtained finished product is a thick mass of light brown color with a yellow tint, with a sweet taste and a pleasant specific smell, soluble in cold water, slightly soluble in 96 95 ethanol.

Example 2

The hypoglycemic activity of a thick extract from yacon root tubers was studied on 35 non-lily female rats weighing 250-295 g. The first group of animals was a control (intact control - IR). Insulin resistance was modeled by subcutaneous injection of dexamethasone (KAKA, Slovenia) at a dose of 0.125 mg/kg once a day (control pathology - KP) for 14 days - the second group of animals. The third group consisted of animals that received an aqueous solution of a thick extract of yacon root tubers at a dose of 250 mg/kg intragastrically once a day against the background of KP; the fourth and fifth groups consisted of animals that on the background of KP received comparative drugs: metformin in the form of tablets "Siofor" BERLIN-CHEMI AG (MENARINI GROUP) and the official herbal collection "Arfazetin" (PJSC "Viola", Ukraine) in doses of 100 mg/kg and 9 ml/kg, respectively, which were administered in similar modes. The blood glucose level was measured with a VIOMIME series glucometer

Widniewie M 550 (Switzerland). All animals were kept in standard vivarium conditions (natural alternation of light and darkness, air temperature 2122 "С, relative humidity 55-70 95) and received complete ration granulated food for rats 4 times a day and drinking water without restrictions (feeding was stopped after 12 h. before blood sampling to determine glycemia).

Blood glucose content was determined on day 1 (baseline, before dexamethasone administration), as well as on the 7th and 14th days of the experiment.

The results of determining the glycemia of rats of all groups and the analysis of the obtained data are shown in Table 1.

Table 1

Hypoglycemic activity of a thick extract of yacon root tubers on the model of dexamethasone hyperglycemia in rats (M-a-t, p--7)

Animal group Initial level Difference level Difference level, Glycemia relative Glycemia relative mmol/l mmol/l baseline level, mmol/l (baseline level,

Cho Cho yacon pathology, 250 mg/kg

Notes: 1. "- statistically significant differences (p" 0.05) relative to the initial level in each group 2. " statistically significant differences (p" 0.05) relative to control pathology (dexamethasone without correction)

Z. and - statistically significant differences (p<0.05) relative to metformin

The results of the research showed a reliable increase in the basal (after 12-hour fasting) blood glucose level of KP rats on the 7th day (6.0420.21 mmol/l) and on the 14th day (6.43:20.17 mmol/l) ) relative to the normal initial level (4.5920.13 mmol/l). In IR animals, the glycemia level was within the normal range relative to the initial data: 5.0020.1 mmol/l on the 7th day and 5.09:20.07 mmol/l on the 14th day.

Zo In rats, which on the background of KP received an aqueous solution of a thick extract of yacon root tubers at a dose of 250 mg/kg orally, the level of glycemia was lower than the parameters of KP both on the 7th and on the 14th day. Thus, on the 7th day of the experiment in the animals of this group, the average level of glycemia increased by 16.57 95 relative to the initial level and amounted to (5.7350.25) mmol/l, which is 15.21 95 lower than the dynamics of the increase in the blood glucose level in the group of KP rats, where the glucose level increased by 31.78 95 relative to the baseline. On

On the 14th day of the experiment, the average value of glycemia in rats that received the studied yacon extract on the background of KP was (6.30:20.32) mmol/l, which is 11.99 95 lower than the dynamics of the increase in the blood glucose level in the group of rats KP, where the glucose level increased by 40.19 95 relative to the baseline.

The decoction of the "Arfazetin" collection had a minor effect on the dynamics of the increase in the level of glucose in the blood of rats, and the difference in the relative initial level was "28.88 95" on the 7th day, -39.42 95 on the 14th day, which was practically equal to the CP indicators (31.78 95 and 440.19 95 respectively).

The thick yacon extract showed pronounced hypoglycemic activity, the degree of which it approached the synthetic hypoglycemic drug metformin, the difference in the relative initial level of which on the 7th day of the experiment was 16.57 95, on the 14th day - 428.20 9.

The increase in the level of blood glucose relative to the baseline in rats administered metformin was 14.93 95 and 421.13 95 on the 7th and 14th day, respectively.

Therefore, the use of an aqueous solution of a thick extract of yacon root tubers contributed to a reliable increase in glucose tolerance in an experiment on rats based on the model of chronic dexamethasone hyperglycemia. This substance is promising for the creation of a new herbal medicine with a pronounced hypoglycemic effect.

Sources of information: 1. Fructose and phenolic compounds of yacon plants / M.S. Gine, V.K. Gins, P.F. Kononkov et al. / Vegetables of Russia. - 2003. - Mo. 3. - P. 118-122. 2. Myshchenko L.T., Dunych A.A., Veselsky S.P., Sereda A.V. Sugar-lowering effect of extracts of medicinal plants and their collections in alloxan-induced diabetes mellitus. Bulletin of Luhansk National University named after Taras Shevchenko.

Biological sciences. 2012, 17(252): 109-115.

Z. Vagopi 5. Zi2yKi-Ketteitveieg R., Sara!to2-Avzei 5. M., Sytap V.K.M., Vegzapi-Atado S.A.

Enesi oi shyde ehigasiv oi Ieakhe5 oi ZtaiPapiyiv zopspoiishv5 (uasop) op diusetia ip aiabeiiys gaiv.

Vemivia Vgaziiviga de Siepsia5 Haptaseciisa5, 2008, 44(3): 521-530. 4. Patent Mo 48031, Ukraine, AbIK 36/48. The method of obtaining means that have hypoglycemic activity / V.M. Kovalev, A.B. Sedova, V.I. Dikhtyaryov, O.G. Sitnik The applicant and

From the patent holder Nfau. - Mo 4387530/5)), application 03.03.1988; published 15.08.2002, Bull. Mo 8.

Claims (1)

USEFUL MODEL FORMULA The method of obtaining a substance with hypoglycemic action by extraction of plant material with hot water followed by filtration, evaporation and drying, which differs in that yacon root tubers are used as plant material, extraction is carried out at a ratio of raw material: extractant 1:8.