TWI387460B - Extraction methods and compositions to ameliorate hyperlipidemia, hyperglycermia and fatty liver - Google Patents

Description

Multi-effect compound food combination with blood sugar lowering blood fat and fatty liver and extraction method of each material thereof

Formulation combination of health food and extraction method of each material thereof

In developed countries, lipids and carbohydrates are poorly metabolized, and they are prone to symptoms of metabolic syndrome, such as diabetes, hypertension, hyperlipidemia, arteriosclerosis, and high triglycerides. However, recent studies have found that these diseases are It is associated with receptor-PPARs (liposome-activated proliferating receptors) in the nucleus. Many scientists are currently looking for drugs that activate PPARs as metabolic syndromes. For example, thiazolidinediones (TZD) (insulin sensitizer) has the effect of lowering blood glucose by activating PPARγ, while fibrates (phenoxy aryl) In order to activate PPARα and have the effect of lowering blood fat, but because all of the above are drugs, and have side effects, it is not suitable for long-term consumption.

In recent years, a small amount of literature has been used to reduce blood glucose by activating PPARγ, and it is expected that these natural ingredients can be used to find stronger compounds that can activate PPAR for drug use. Development. At present, the materials in the literature are mainly unilateral. For example, hop extracts can simultaneously activate PPARα and γ, bitter melon extract can simultaneously activate PPARα and γ, licorice extract activates PPARγ, etc., but these active ingredients often need to pass through. Special organic solvents are extracted to obtain effective ingredients. However, in the concept of food, organic solvent extraction is not suitable for food. Therefore, this patent is to use the combination of special alcohol and / or water and the best combination of materials to obtain the most effective food formula for blood lipids, blood sugar, fatty liver.

Many of the drugs for lowering blood sugar and lowering blood fat have been developed through the activation of PPAR α and γ. Therefore, the principle of efficacy and mechanism has been confirmed by many experts and scholars, but this type of medicine is often caused by chemically synthesized compounds. The subject produces liver or kidney toxicity, which makes the body's GOT (breast amino acid acetic acid transaminase) and GPT (brome amino acid pyroglucan amino acid) values higher, so the idea of this patent is to use the medical community to find activation. The method of activating substances is to screen the materials which are commonly eaten by humans in foods, and to find out those having activated PPAR α and γ natural compounds or compound combinations for the benefit of more people.

The present invention is a multi-effect compound food combination and a method for extracting the same, which are characterized in that the ratio of addition is 20~40% of bitter gourd, 1%~10% of licorice, 15%~30% of green algae. Soy protein powder 20~40%. The extraction methods of each material extraction are as follows:

A, bitter gourd extract: 100 grams of fresh bitter gourd juice extracts bitter gourd juice, the remaining residue is then soaked in 100 ~ 200 ml 90% alcohol for 6 to 8 hours, filtered to remove residue, bitter gourd juice and alcohol The extract is mixed and freeze-dried to form a powder.

B, licorice extract: 100 grams of licorice after pulverization, add 500 ~ 1000ml volume of pure water at 60 ~ 100rpm shaking room temperature for more than 10 hours, remove the licorice residue, the supernatant is freeze-dried into a powder, this powder The mixture is further immersed in 50-100 ml of methanol for more than 20 hours, and the alcohol-soluble portion is further dried into a powder.

C, broken wall green algae: 100 grams of broken green algae powder, 500 ~ 1000 ml 100 ° C boiling water for 60 ~ 180 minutes, the aqueous solution is filtered with 10ml - 5 mm molecular sieve, remove the residue part, the aqueous solution part Concentrated and dried powdered.

Ding, soy protein powder: 100 grams of deoiled soybean meal, with 300~500ml of water, adding 0.3%~3% proteolytic enzyme endopeptidase (EC3.4.24.28), acting at 45-50 degrees for 6-8 hours, after Filtration, taking the filtrate, and filtering with a molecular sieve to obtain a component of 10,000 Dalton or less.

The above formula is used for animal and human test, which proves that it has the effects of lowering blood fat, blood sugar and improving fatty liver, and the combination of the above processes has good water diffusion property, and other compound food combinations can be applied, including Development of capsule lozenges, cereal flour, beverages, and oral liquid products.

Using the transactivation assay system (transfected cell activation system) that has been established to transfect PPAR alpha and PPAR gamma - to activate the PPAR promoter gene, access the luminescent gene, and then send the gene into The liver cells are expressed, and then the material is used to stimulate the cells. When the material has the ability to activate PPAR, the intensity of cold light can be measured by a luminometer to determine the effect of the material on the activation of PPAR α and PPAR γ. Decide on the process and match the combination.

After screening a lot of food materials, the following four materials were found to be very active, and different extraction methods were carried out to find out the following patented processes, which have the best activated PPAR effect. The manufacturing methods are as follows:

A, bitter gourd extract: 100 grams of fresh bitter gourd first extract the bitter gourd juice with a juicer, and the remaining residue is soaked in 100~200 ml 90% alcohol for 6-8 hours, filtered to remove the residue, and the bitter gourd juice and The alcohol extract is mixed and freeze-dried to form a powder.

B, licorice extract: 100 grams of licorice after pulverization, add 500 ~ 1000ml volume of pure water at 60 ~ 100rpm shaking room temperature for more than 10 hours, remove the licorice residue, the supernatant is freeze-dried into a powder, this powder and then Soak in 50-100 ml of methanol for more than 20 hours, and then dry the alcohol-soluble portion into a powder.

C, broken wall green algae: 100 grams of broken green algae powder, 500 ~ 1000 ml 100 ° C boiling water for 60 ~ 180 minutes, the aqueous solution is filtered with 10ml - 5 mm molecular sieve, remove the residue part, the aqueous solution part Concentrated and dried powdered.

Ding, soy protein powder: 100 grams of deoiled soybean meal, with 300~500ml of water, adding 0.3%~3% proteolytic enzyme endopeptidase (EC3.4.24.28), acting at 45-50 degrees for 6-8 hours, after Filtration, taking the filtrate, and filtering with a molecular sieve to obtain a component of 10,000 Dalton or less.

Then carry out the combination and matching of the compound, and use the materials extracted by the above four patent processes to find the best dose-inducing activity. In addition, the material arrangement and combination are also carried out. The experimental results show that the four materials are matched together and pass the PPAR. The screening method for α and PPAR γ cell lines has better ability to activate PPAR α and PPAR γ than any combination of unilateral or other formulations (compared to the same total concentration) (see Figure 1 and Figure 2 below). Figure 1: shows the multiple of activated PPAR α, and Figure 2 shows the multiple of activated PPAR γ. The control group was the result of only stimulating the cells with water, and the activation factor was 100% (1 time), while the highest activation degree of PPARα was only 1.5, 1.4, 2.3, 2.1 times for licorice, bitter gourd, green algae and soybean. When the four components are combined, the highest activation degree of PPARα can be as high as 5.3 times, and the maximum activation degree of four unilateral PPARγ is 2.9, 1.3, 1.8, 1.9 times, respectively. When the four components are combined, the PPARγ is combined. The highest activation degree is up to 5.0 times. The above results confirm that the compound combination has the effect of adding PPARα and γ, which is superior to the single combination and has progress.

The above formula combination is further tested in vivo for blood lipids, blood sugar and fatty liver to further confirm its efficacy

■ Blood Lipid Blood Sugar Animal Experiment: C57BL/6 strain mice purchased from Chengda Animal Center, induced by high-fat diet, cause hyperglycemia and hyperlipidemia in mice. After we designed the mice, we can find the mice. After 3 weeks of feeding, it had the effect of lowering blood sugar (14.6%) and triglyceride (21.8%), and in the sixth week, the effect of lowering cholesterol (12.4%) was observed.

■ Blood lipids Blood sugar human experience: In the blood sugar experience part, after one month of taking, the average blood glucose level decreased from 99.1 mg/dl to 94.3 mg/dl (a decrease of 5%). In the blood lipid experience part, after one month of testing, the average cholesterol value of all experiencers decreased from 233 mg/dl to 207 mg/dl (a decrease of 11.1%); while the average value of triglycerides was 221 mg/dl. Dropped to 162mg/dl (down 26.7%), the experience has a 100% satisfaction. During the experience period, the experiencer's liver index (breast amino acid acetic acid transaminase and bran amino acid pyrogal aminotransferase) and kidney index (creatinine) did not rise or even decreased, confirming that the patented formula would not cause liver and kidney. The burden. When the experiencer does not continue to take the patented formula (more than one month), it can be seen that the blood sugar and blood lipids (except triglycerides) of the experiencer have rebounded, confirming that the lowered blood sugar and blood lipids are the effects of the patented formula.

■ Fatty liver animal experiment: C57BL/6 strain mice purchased by Chengda Animal Center were induced by high fat and high fructose feed, and alcohol was added to the drinking water to make the rats develop symptoms of fatty liver. In mice, it can be found that after 2 months of feeding, the rats can reduce the liver index (brome amino acid pyramid aminotransferase), and the transglutaminase amino acid enzyme index of the patented formula mouse is reduced from 50 U/L to 36 U/L. The transaminase index of the bran amino acid coke grape without the patented formula control group increased from 51 U/L to 55 U/L. In addition, the rats were sacrificed. The liver of the mice was found to have an average of 23 mg of liver triglyceride in the formula-treated mice and 28 mg in the control group without the formula, and there was a significant difference in the abdominal and subcutaneous fat of the mice. The patented formula is significantly lower than that not taken, so this patented formula has the effect of reducing fat accumulation.

■ Fatty liver human experiment: This patented formula for human experience, after three months, you can see the effect of the reduction of the average biochemical value, including bran amino acid grass acetic acid transaminase (from 46U / L down to 30U / L) , Bran amino acid Jiao grape transaminase (from 45U / L down to 35U / L), triglyceride (from 204mg / dl down to 169mg / dl), total cholesterol (from 220mg / dl down to 200mg / Dl) has obvious improvement, even a number of experiencers can improve the moderate fatty liver judged by ultrasound to mild or mild to moderate fatty liver within 3 months.

At present, this patented formula is different from the products that only act on the gastrointestinal tract and inhibit the absorption of oil, and there is no product in the market that simultaneously appeals to lowering blood sugar and lowering blood fat and improving fatty liver. Therefore, this patent formula and its materials are The extraction method is a patent element with novelty and progressiveness and industrial utilization.

Claims (5)

A multi-effect compound food composition having blood fat lowering and improved fatty liver, characterized in that the multi-effect compound food composition is obtained by the following method; (1) bitter gourd extract: 100 g fresh bitter gourd first juiced The bitter gourd juice is extracted by the machine, and the remaining residue is soaked in 100~200 ml of 90% or more alcohol for 6 to 8 hours, and then the residue is removed by filtration, and the bitter gourd juice and the alcohol extract are mixed and freeze-dried to obtain a powder. (2) Licorice extract: After crushing 100 g of licorice, add 500-1000 ml of pure water and soak for 60 hours at 60-100 rpm for 10 hours or more. After removing the licorice residue, the supernatant is freeze-dried into a powder. The powder is further immersed in 50-100 ml of methanol for more than 20 hours, and the alcohol-soluble portion is further dried into a powder; (3) Broken green algae: 100 g of broken green algae powder, with 500-1000 ml of 100 ° C boiling water After taking 60~180 minutes, the aqueous solution is filtered by molecular sieve at 10mm-5mm to remove the residue, and the aqueous solution is partially concentrated and dried to be sprayed; (4) Soy protein powder: 100g of deoiled soybean meal, 300 ~500ml of water, adding 0.3% to 3% protein decomposition Su endopeptidase (E.C.3.4.24.28), under the action of 45-50 for 6-8 hours, filtered, the filtrate, was collected by filtration to 10000 Dalton molecular sieve following components. A multi-effect combination food composition having blood fat lowering and improved fatty liver according to claim 1 of the patent application can simultaneously activate PPAR α (hypolipidemic) and PPAR γ (hypoglycemia), and has an improved fatty liver. efficacy. A multi-effect combination food composition having a blood lipid lowering and improved fatty liver according to the first aspect of the patent application, the product type comprising: capsules, tablets, powders and oral liquids. A method for extracting a multi-effect compound food composition having blood fat lowering and improving fatty liver includes: (1) bitter gourd extract: 100 g fresh bitter gourd first extracts bitter gourd juice with a juicer, and the residual residue is further 100-200 After soaking for more than 90% of the alcohol for 6 to 8 hours, the residue is removed by filtration, and the bitter gourd juice and the alcohol extract are mixed and freeze-dried to obtain a powder. (2) Licorice extract: After pulverizing 100 g of licorice, add 500-1000 ml of pure water and soak for 60 hours at 60-100 rpm for 10 hours or more. After removing the licorice residue, the supernatant is freeze-dried into a powder. The mixture is further immersed in 50-100 ml of methanol for more than 20 hours, and the alcohol-soluble portion is further dried into a powder. (3) Broken green algae: 100g broken green algae powder, extracted with 500~1000 ml 100 °C boiling water for 60~180 minutes, the aqueous solution is filtered with molecular sieve at 10mm-5mm to remove the residue part, the aqueous solution part It is made by concentration and drying. (4) Soy protein powder: 100 grams of deoiled soybean meal, with 300~500ml of water, adding 0.3%~3% proteolytic enzyme endopeptidase (EC3.4.24.28), and acting at 45-50 degrees for 6-8 hours, After filtration, the filtrate was taken and filtered through a molecular sieve to obtain a component of 10,000 Dalton or less. PPAR α (hypolipidemic) and PPAR γ (hypoglycemia) can be activated simultaneously with an extraction method of a multi-effect compound food composition having blood fat lowering and improved fatty liver according to item 4 of the patent application scope. The effect of fatty liver.