TW202217308A - Apparatus for accelerating and performing uniformly reaction of to-be-reacted substances and reactants contained in wettable substrate, system containing the apparatus, and coater - Google Patents

Abstract

The present invention relates to an apparatus for accelerating and performing uniformly reaction of to-be-reacted substances and reactants contained in wettable substrate. The apparatus includes a machine body having a drainage plate for placing the wettable substrate and a coater configured on the top of the machine body, wherein the coater maintains a predetermined height for moving along the wettable substrate in horizon direction, and the coater has one or a plurality of slit. One end of the slit has an injection opening without limitation of arranged at the center of geometric symmetry, and the other end of the slits has a liquid discharge opening.

Description

Equipment for accelerating and uniform reaction of reactants and reactants contained on wettable substrates, systems containing the same, and coating heads

The present invention relates to a device and system for accelerating reactions on wettable substrates, especially for biochemical and immunological reaction tests.

In recent years, the market demand for biochemical testing has continued to rise and it is widely used, and its application in medical testing is particularly significant and important. How to quickly and stably provide reliable test results is still a problem that has not been fully solved for many years; especially in the outbreak of the new crown pneumonia epidemic, screening technology has become one of the first goals of public health research and development in countries around the world.

Under the strategy of pursuing low cost and rapid market penetration, many rapid screening test tablets will continuously improve the reagent formula to achieve the purpose of rapid screening of diseases. However, under this strategy, it often happens that different rapid screening test pieces are used for the same disease, but the detection results are contradictory, which causes the problem of epidemic prevention in public health.

Compared with the quick screening test piece, although the test results of the laboratory screening method are highly reliable, the operation requires a lot of time and manpower. Taking the traditional western blotting method as an example, its preparatory work is cumbersome, and a considerable amount of antibodies and reagents need to be used for the reaction, and the reaction time is long. If the antibody concentration is not well controlled, the background value is too high and the target reactant cannot be distinguished, and the detection must be repeated.

In view of the above-mentioned shortcomings, the present invention intends to provide a device for accelerating and uniformly reacting a reactant and a reactant contained on a wettable substrate, which comprises a coating head and a liquid discharge bottom plate, both of which are provided by Capillary force can reduce the amount of reagents and reactants/reactants (such as antibodies), accelerate the reaction of reactants and reactants, and reduce the reaction time, so as to improve conventional screening tests, which require a lot of time and manpower. In addition, the device of the present invention can be matched with a system module and a test piece with one or more target reactants pre-fixed, so as to provide useful disease detection information, that is, the development of the patient's disease and the corresponding clinical statistical information in real time and objectively, to Assist medical personnel to make correct diagnosis and treatment.

Therefore, the object of the present invention is to provide a device for accelerating and uniformly reacting a reactant and a reactant contained on a wettable substrate, which comprises a machine body and a coating head. The machine body has a drain bottom plate for the wettable substrate to be placed on; the coating head is arranged above the machine body and can maintain a preset height along the wettable substrate Moving in the horizontal direction, the coating head has one or more slits, one end of the slit has an injection opening, and the other end of the slit has a liquid discharge port.

Further, the wettable substrate may be a porous material.

Further, the apparatus further includes a suction generating device, which is connected to the drainage bottom plate to provide a negative pressure to the wettable substrate.

Further, the drain bottom plate is made of a porous material or a non-porous material, and when the liquid drain bottom plate is made of the non-porous material, the liquid drain bottom plate is provided with micro-channels on its surface.

Further, the groove depth of the microchannel has a gradient.

Further, an oscillator is further provided below the liquid discharge bottom plate.

Further, a waste liquid storage tank is further provided between the liquid discharge bottom plate and the suction generating device.

Further, the coating head can be further directly connected to a cleaning liquid storage tank.

Further, both sides of the slit are non-parallel.

Further, at least one side of the drain bottom plate has a coating preparation area.

Another object of the present invention is to provide a system for rapidly calibrating a reactant, which includes the device of the present invention and an optical image capturing and analysis module.

Further, the optical image capturing and analyzing module includes a camera, and the camera can be a photosensitive element module such as CCD or CMOS.

Further, the system includes use with a test strip pre-fixed with one or more target reactants.

Further, the system includes a non-transitory machine-readable medium, which stores a program for interpreting the result of the test strip after the reaction, and the non-transitory machine-readable medium can be selected from any one or two or more from the following: A group composed of modules: an image interpretation module, a certain amount of modules, a reference database module and a comparison module. The image interpretation is used to interpret the reacted image of the test piece; the quantitative module is used to quantify the content of the target reactant of the test piece; the reference database module is used to store the target reactant of the test piece The detection map and related clinical symptoms; the comparison module is used to compare the image with the detection map to determine the result after the test piece reacts.

Another object of the present invention is to provide a coating head, which has one or more slits, one end of the slit has an injection opening located at the center of geometric symmetry without restriction, and the other end of the slit has a liquid discharge port . In a preferred embodiment, the two sides of the slit are non-parallel.

Compared with the prior art, the advantages of the present invention are:

The equipment of the present invention is matched with the coating head of the present invention and can be moved manually or electrically as needed, and can be matched with liquid discharge bottom plates of different materials and designs and matched with the suction generating device as required, by adding an oscillator or the like. , reducing the required operating time.

The system of the present invention facilitates one-stop rapid screening and imaging, and can be used as a biomedical detection system with high accuracy and efficiency, which is different from the conventional western ink dot method, which requires multi-station, long time and time-consuming. manpower; in addition, the test strips matched with the system and the reference database in the non-transitory machine-readable media contain the relevant clinical symptoms corresponding to the detection pattern, replacing the current rapid screening that only judges whether there is infection by a single reactant. . Therefore, the system of the present invention can provide useful disease detection information in real time and objectively under various circumstances, that is, the development of the patient's disease and the corresponding clinical statistical information, so as to assist medical personnel to make correct diagnosis and treatment.

The coating head of the present invention can be designed as one or more slits, and the capillary force and gravity can be used to disperse the liquid evenly to both sides and downward without additional power. Simultaneously test whether a variety of different reactants can react with the reactants adsorbed on the wettable substrate, which greatly improves the detection efficiency.

Embodiments of the present invention will be described below to describe the present invention with reference to the accompanying drawings. In the drawings, the same reference numerals denote the same elements, and the size or thickness of the elements may be exaggerated for clarity of illustration.

In the present invention, the term "wettable substrate" refers to a membrane that can adsorb reactants in biochemical or immune reactions, usually porous, such as but not limited to the substrate film used in Western blotting. The material can be, for example, But not limited to poly(vinylidene fluoride, PVDF) or nitrocellulose (Nitrocellulose, NC).

In the present invention, the term "reactant" refers to a target substance (such as but not limited to antibodies or antigens, tissue cells, etc.) adsorbed or immobilized on a wettable substrate for subsequent antigens or antibodies or other The effect of another chemical substance with which it interacts. "Reactant" refers to a substance (such as, but not limited to, antigens or antibodies, drugs, drugs, pathogens, body fluids, environmental collection fluids, etc.).

In the present invention, the suction generating device includes a "negative pressure vacuum device" and a liquid absorbing material with capillary force; the "suction generating device" refers to a device that generates a negative pressure, which can generate a suction force to extract the liquid. Since the direction of the extraction force and the coating direction of the coating head are not parallel, a forced convection is generated, forcing the reactant coated on the porous material to move toward the direction of the reactant.

As shown in FIGS. 1 to 4 , an embodiment of the present invention is an apparatus for accelerating and uniformly reacting a reactant and a reactant contained on a wettable substrate, which includes a machine body 1 , a coating head 2 and a suction generating device 3; the machine body 1 has a liquid discharge bottom plate 101 for the wettable substrate M to be placed on, and the coating head 2 is arranged on the machine body 1, and can maintain a preset height to move in a horizontal direction along the wettable substrate M; as shown in FIGS. 2 and 3, the coating head 2 can have one or more slits 201, the slits One end of 201 has an injection opening 202, the other end of the slit 201 has a liquid discharge port 203, and both sides of the slit 201 are non-parallel; as shown in FIG. 1, the suction generating device 3 is connected to the row The liquid bottom plate 101 (connected via line P) provides a downward suction for the wettable substrate M.

When the apparatus of the present invention is used for coating, if necessary, a quantitative tool or an electric injection pump can be used arbitrarily to inject the reactant into the coating head 2, that is, from the injection opening 202 to the slit 201. In; the reactant naturally forms a liquid bridge between the liquid discharge port 203 and the wettable substrate M by capillary force, gravity and inertial force; when the coating head 2 moves horizontally, the reactant is used The balance between the viscous force, capillary force, gravity and inertia force of the reactant can be pulled out of the liquid bridge of the reactant laterally for coating. It is generally believed that the coating head 2 needs to form a diversion structure in the two flat plates so that the injected liquid can flow downward; however, the inventor's experiment found that the coating head 2 only needs to form the slit 201 with the two flat plates, and it is relatively If the two sides of the slit 201 are not flat, the capillary force and gravity can be used to disperse the liquid evenly to both sides and downward without additional power (such as the power provided by an injection pump). In the embodiment of FIG. 1, the suction generating device 3 is included to further shorten the reaction time and reduce the noise caused by non-specific binding, but through experiments, in another embodiment, the lower suction generation can be omitted. In the device 3, the reactants in the coating head 2 can still be distributed evenly on the wettable substrate M on which the reactants are adsorbed by capillary force and gravity even though the liquid can be evenly dispersed to both sides and downwards.

The coating head 2 may have one or more slits. The coating head 2 shown in FIG. 3 has only one slit 201. The side of the slit 201 has an injection opening 202 and a liquid discharge port 203 below. The injection opening 202 It does not need to be limited to the center of geometric symmetry, that is, there is no special position limitation. Although the drawing shows that the injection opening 202 is located at the side of the coating head 2, the injection opening 202 only needs to enable the liquid to flow to the slit 201, so it can also be used. Set on the top of the coating head 2 or on the side surface, etc.; and the liquid discharge port 203 forms an entire opening under the slit 201 of the coating head 2, so that the reactants in the coating head 2 can be uniformly coated. cloth on the wettable substrate M; the state of the coating head 2 in FIG. 2 is a plurality of slits 201, the plurality of slits 201 are independent channels that are not connected to each other, and the upper end of each slit 201 is There is an injection opening 202, and the lower end of each slit 201 has a liquid discharge port 203. Since the plurality of slits 201 are not communicated with each other, different reactants can be injected into each injection port 202, so that one reaction can be performed in one reaction. , testing whether a variety of different reactants can react with the reactants adsorbed on the wettable substrate M, greatly improving the detection efficiency.

In the apparatus of the present invention, the coating head 2 can be moved in the horizontal direction manually or automatically. As shown in FIG. 1 , in one embodiment of the present invention, the apparatus of the present invention may further comprise a cleaning liquid storage tank 5 , the coating head 2 is connected to the cleaning liquid storage tank 5 , and the coating head 2 can The sliding rail 6 under the cleaning liquid storage tank 5 is moved horizontally. The driving force of the sliding rail 6 can come from an electric motor or manual operation. The present invention does not limit the setting position and driving method of the sliding rail 6 . Simple and flexible, the coating head 2 is manually moved in the horizontal direction, eliminating the need for a horizontal displacement electric motor and peripheral control auxiliary system equipment, thereby reducing costs and increasing operational convenience.

Another embodiment of the apparatus of the present invention is shown in FIG. 4 . In the apparatus of the present invention, the coating head 2 can perform horizontal coating on the wettable substrate M by manual or automatic methods, and then auxiliary The wettable substrate M is manually or automatically moved to a shaking cleaning tank, an ultrasonic cleaning tank, or a peripheral auxiliary system equipment of an optical image capture and analysis module to reduce costs and increase operational convenience.

In the apparatus of the present invention, the drain bottom plate 101 refers to a substrate on which the wettable substrate M can be placed, and the material of the drain bottom plate 101 can be a porous material or a non-porous material; for example, the drain bottom plate 101 is a porous material (such as ceramics, foam, paper, porous fibers, etc.), then the drainage bottom plate 101 has a downward suction effect due to its porousness, and can wet the liquid containing the reactant on the substrate M. Suction downward, inhibit the lateral diffusion of the reactants, promote the continuous dynamic reaction of the reactants and the reactants, and reduce the waiting time for the reaction after coating; if the drain bottom plate 101 is a non-porous material, the material can For example, glass, metal, acrylic, polymer, silicone, plastic or a combination thereof, etc., and in a preferred embodiment, the drain bottom plate 101 can be as shown in FIGS. 5 to 7 , the drain bottom plate 101 It has the micro-channel 1011; the micro-channel 1011 is small and has capillary force, and in a preferred embodiment, the groove depth of the micro-channel 1011 has a gradient (in other words, the groove depth can be from shallow to deep (or from deep to shallow), the liquid in the microchannel 101 can flow due to the difference in capillary force, and the relatively low part is located at one end with the liquid discharge port 1012, and the liquid discharge port 1012 is used to Liquid discharge, wherein the liquid discharge port 1012 can be one or more than two, as shown in FIG. 5, there are two liquid discharge ports 1012; in another embodiment, the liquid discharge port 1012 can be connected to a suction generating device; Preferably, the liquid discharge port 1012 is disposed at the lower part of the groove depth of the micro-channel 1011; Coating is carried out in the direction from high to low; due to the capillary force and the height difference of the flow channel, the liquid in the micro-channel 1011 flows in a directional direction (that is, it flows towards the end with the liquid discharge port 1012), which not only can In addition to facilitating the collection of the flowing liquid, the directionality of the liquid flow can also drive the liquid of the reactant on the wettable substrate M to be attracted downward. Further, when the drainage bottom plate 101 is matched with the negative pressure generated by the suction force generating device 3, it has a suction force, which can be multiplied by the downward suction effect, thereby further accelerating the reaction time of the reactants and the reacted objects.

In a preferred embodiment, the drain bottom plate 101 can also preferably be made of non-porous material. When the drainage bottom plate 101 is made of a porous material (such as foam, paper, porous fiber, metal mesh, etc.), since the porous material itself has pores and thus has suction, the drainage bottom plate 101 as a whole continues to absorb the liquid. Wetting the liquid on the substrate M, the wettable substrate M cannot maintain a certain degree of wetness, so if it is not treated in time, it may affect the subsequent interpretation results; in addition, the pores of the porous material are prone to residual reactants, which cannot be cleaned. It can be reused afterward; however, the drainage bottom plate 101 made of porous material can still be used in a disposable and instantly interpretable reaction procedure. In contrast, when the liquid discharge bottom plate 101 is made of non-porous material, as shown in FIGS. 5 to 7 , because the microfluidic channel 1011 uses capillary force and gradient height difference, the liquid of the reactant flows to the discharge After the liquid port 1012 is discharged, the liquid drain bottom plate 101 has no pores as a whole, so it will not quickly absorb the liquid containing the reactant on the wettable substrate M, so the wettability of the wettable substrate M will not be insufficient. That is, it is maintained in a state of containing liquid but the liquid does not disappear too quickly). In addition, the non-porous material of the drain bottom plate 101 can be cleaned and reused, which is more environmentally friendly. Therefore, the drain bottom plate 101 can also be made of suitable materials according to the needs of the user, or can be made into a disassembled module for convenient installation and replacement. In addition, as shown in FIG. 2 , a coating preparation area 1013 can be further set on the liquid discharge bottom plate 101 , and the coating head 2 can be moved horizontally to the wettable base after forming a stable liquid bridge in the coating preparation area 1013 . Coating is performed on the material M to maintain the set coating spacing and to prevent the coating head 2 from directly contacting the wettable substrate M and causing contamination. The coating preparation area 1013 may be formed integrally with the drain bottom plate 101 , or the coating preparation area 1013 may be additionally disposed on the drain bottom plate 101 .

A further embodiment of the apparatus of the present invention is that the apparatus further includes an oscillator 102 and a waste liquid storage tank 4; as shown in FIG. 1 , the oscillator 102 is disposed under the drainage bottom plate 101; 4 is arranged between the drain bottom plate 101 and the suction generating device 3, and is connected with a pipeline P; the cleaning fluid storage tank 5 is connected to the coating head 2, and the slide rail 6 is arranged to the cleaning fluid storage tank 5 below. The apparatus of this embodiment can clean the wettable substrate M, that is, after the reaction is completed, the cleaning solution in the cleaning solution storage tank 5 is applied to the wettable substrate M through the coating head 2, and the The micro-channels 1011 of the drain bottom plate 101 can properly keep the wettable substrate M wet and filled with cleaning solution. At the same time, the oscillator 102 cleans the wettable substrate M by vibrating to remove excess Reactant. When cleaning excess reactants in conventional laboratory cleaning operations (eg, Western blotting method), the wettable substrate M is moved to a lotion box for immersion, with natural diffusion supplemented by shaking, and repeated for many times. In order to achieve the purpose of cleaning, the process is labor-intensive and lengthy. In contrast, the apparatus of this embodiment can complete the cleaning without moving the wettable substrate M during cleaning, which not only avoids the risk of damage to the wettable substrate M due to movement, but also has an excellent cleaning effect for vibration cleaning. Naturally spread and shake. In addition, when the drainage bottom plate 101 is made of non-porous material, the micro-channel 1011 can make the pressure difference between the upper and lower ends of the wettable substrate M more evenly distributed during pumping, so as to achieve minimal cleaning The liquid can provide the most effective and uniform cleaning effect, thereby reducing the required operating time.

Preferably, the oscillator 102 is an ultrasonic oscillator, which can be automatically activated in the process of cleaning the wettable substrate M, and can perform relatively fast lateral movements of tens of thousands of times per second, so as to improve the cleaning effect and greatly reduce the amount of cleaning. function of time. By the combination of the draining bottom plate 101 and the oscillator 102, the wettable substrate M can be cleaned effectively in a short time, and the background noise in the detection result signal is suppressed to improve the system function and make the signal clearer . Preferably, the apparatus of this embodiment of the present invention utilizes the combination of the drain bottom plate 1011 and the ultrasonic oscillator 102, which can effectively complete the cleaning in a short period of time, and can effectively suppress the background noise in terms of signal presentation. Therefore, the system function can be improved to make the signal clearer.

Using the equipment of the present invention to perform western blotting method can obtain a strong detection signal; as shown in Figure 8, when the reactants are severe acute respiratory syndrome new coronavirus nucleocapsid protein (SARS-CoV-2 NP), severe acute respiratory syndrome Acute respiratory syndrome coronavirus nucleocapsid protein (SARS-CoV NP), Middle East respiratory syndrome coronavirus nucleocapsid protein (MERS-CoV NP) and human coronavirus NL63 nucleocapsid protein (HCoV-NL63), the left picture is used The experimental results of the conventional western ink dot method show that the detected signals are noisy and unclear. In contrast, the right picture shows the experimental results using the device of the present invention, and the detected signals are noise-free and clear.

Another aspect of the present invention is to provide a system for rapidly calibrating a reactant, which includes the apparatus of the present invention and an optical image capture and analysis module. The optical image capture and analysis module includes a camera, which can be used to capture and display the experimental results of the detection (such as Western ink dot method) completed by the apparatus of the present invention; the optical image capture and analysis module may also include an excitation The light source and a filter, the captured image signal can include cold light, UV light, fluorescent light and visible light, which is not limited in the present invention. The system of the present invention facilitates one-stop rapid screening and imaging, and can be used as a biomedical detection system with high accuracy and efficiency, which is different from the conventional western ink dot method, which requires multi-station, long time and time-consuming. manpower.

The camera may include CCD or CMOS, etc., which is not limited in the present invention.

Further, the system of the present invention can be used in conjunction with a test piece pre-fixed with one or more target reactants, which is the wettable substrate M that can be used in the apparatus of the present invention, since the test piece has been One or more target reactants are pre-fixed, and quick screening can be completed quickly when samples containing unknown reactants are to be detected. Preferably, the test strip is pre-fixed with a variety of target reactants, because the test strip with a variety of reactants presents a detection pattern (for example, the experimental results of the Western blotting method), which can replace the conventional system only. A rapid screening for detecting a single reactant to determine whether there is an infection.

Further, the system of the present invention may include a non-transitory machine-readable medium, which stores a program for interpreting the result of the test strip after the reaction, the non-transitory machine-readable medium is selected from any one or two kinds The above is a group consisting of the following modules: an image interpretation, a certain amount of modules, a reference database module and a comparison module. The image interpretation and quantification module is used for interpreting the image of the test piece after reaction. The quantitative module is used to quantify the content of the target reactant of the test piece. The reference database stores the detection pattern and related clinical symptoms of the target reactant of the test piece. The comparison module is used to compare the image with the detection map to determine the result after the test piece reacts. Specifically, through the design and selection of molecular biology and serology, test strips with different detection reactant profiles can be designed; when the test strips are reacted with the device of the present invention, they can be read by the non-transitory machine. The program of the media is interpreted. After the image of the test piece is interpreted by the image interpretation and quantification module, the comparison module compares the image with the detection map stored in the reference database to obtain detection results. In addition, since there are relevant clinical symptoms corresponding to the test pattern in the reference database, if the image and the test pattern have the same results after comparison, the relevant clinical symptoms can also be compared to replace the current only single reactant. A quick screening for simply judging whether there is an infection. Therefore, the system of the present invention can provide useful disease detection information, ie, the development of the subject's disease and corresponding clinical statistical information, in a real-time and objective manner in various situations, so as to assist medical personnel to make correct diagnosis and treatment.

The above detailed description is a specific description of a feasible embodiment of the present invention, but this embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not depart from the technical spirit of the present invention shall be included in the within the scope of the patent of the present invention.

1: Machine body 101: Drain bottom plate 102: Oscillator 1011: Microfluidics 1012: Drain port 1013: Coating Preparation Area 2: Coating head 201: Slit 202: Injection opening 203: Liquid discharge port 3: Suction generating device 4: Waste liquid storage tank 5: Cleaning fluid storage tank 6: Slide rail M: Wettable substrate P: Pipeline

FIG. 1 is a schematic appearance diagram of an apparatus (1) of the present invention for accelerating and uniformly reacting a reactant and a reactant contained on a wettable substrate.

FIG. 2 is a schematic view of the appearance of the coating head and the liquid discharge bottom plate of the apparatus of the present invention.

Figure 3 is an oblique side view of the coating head of the apparatus of the present invention.

4 is a schematic diagram of the appearance of a coating apparatus (2) of the present invention for accelerating and uniformly reacting a reactant and a reactant contained on a wettable substrate.

FIG. 5 is a schematic view of the appearance of the non-porous material drainage bottom plate (1) of the present invention.

FIG. 6 is a schematic appearance diagram of the non-porous material drainage bottom plate (2) of the present invention.

FIG. 7 is a schematic view of the appearance of the non-porous material drainage bottom plate (3) of the present invention.

Figure 8 is a graph of the experimental results of the western ink dot method: the right picture is completed by the equipment of the present invention; the left picture is completed by the conventional western ink dot method.

1: Machine body

101: Drain bottom plate

102: Oscillator

2: Coating head

3: Suction generating device

4: Waste liquid storage tank

5: Cleaning fluid storage tank

6: Slide rail

P: Pipeline

Claims (15)

A device for accelerating and uniformly reacting a reactant and a reactant contained on a wettable substrate, comprising: a machine body having a drain bottom plate on which the wettable substrate is placed; and A coating head is installed above the machine body and can maintain a predetermined height and move in a horizontal direction along the wettable substrate. The coating head has one or more slits, one end of the slits There is an injection opening, and the other end of the slit has a liquid discharge port. The apparatus of claim 1, wherein the apparatus further comprises a suction force generating device, the suction force generating device is connected to the drainage bottom plate to provide a suction force to the wettable substrate. The device of claim 1, wherein the drain bottom plate is made of a porous material or a non-porous material, and when the liquid drain bottom plate is made of the non-porous material, the drain bottom plate is provided with micro-channels on its surface. The apparatus of claim 3, wherein the groove depth of the microfluidic channel has a gradient. The apparatus of claim 4, wherein a waste liquid storage tank is further provided between the liquid discharge bottom plate and the suction generating device. The device of claim 5, wherein a vibrator is further provided below the draining bottom plate. The apparatus of claim 6, wherein the coating head is further connected to a cleaning fluid storage tank. The apparatus of claim 1 , wherein at least one side of the drain bottom plate has a coating preparation area. The apparatus of claim 1, wherein the two sides of the slit are non-parallel. A system for quickly calibrating a reactant, which comprises the device according to any one of claims 1 to 9 and an optical image capture analysis module. The system of claim 10, wherein the optical image capture analysis module includes a camera. The system of claim 11, further comprising cooperating with a test strip pre-fixed with one or more target reactants. The system of claim 12, further comprising a non-transitory machine-readable medium storing a program for interpreting the result of the reaction of the test strip, the non-transitory machine-readable medium being selected from any one or two more than one group consisting of the following modules: An image interpretation, used to interpret the image of the test piece after the reaction; A certain amount of module is used to quantify the content of the target reactant of the test piece; a reference database module, storing the detection pattern and related clinical symptoms of the target reactant of the test piece; and A comparison module is used to compare the image with the detection map to determine the result after the test piece reacts. A coating head has one or more slits, one end of the slit has an injection opening which is not limited to be placed at the center of geometric symmetry, and the other end of the slit has a liquid discharge port. The coating head of claim 14, wherein both sides of the slit are non-parallel.

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