TW201628598A - Composition comprising gooseberry or glutathione - Google Patents

Abstract

The present disclosure relates to a composition for skin whitening, which contains one or more of a gooseberry extract and glutathione as an active ingredient. In the composition, the gooseberry extract may be an extract of the fruit of gooseberry and the composition of the present disclosure may be a composition for skin whitening, which contains the gooseberry fruit extract and the glutathione as active ingredients. Due to the synergic effect of the gooseberry fruit extract and the glutathione, the composition of the present disclosure exhibits very superior effect of reducing melanin production and inhibiting tyrosinase activity and, therefore, provides a skin whitening effect.

Description

Composition comprising gooseberry or glutathione

The present invention relates to a composition comprising gooseberry extract or glutathione.

Melanin is a pigment that determines the color of human skin. Skin color is determined by the amount and distribution of melanin. Melanin is produced by cells called melanin located at the bottom of the epidermis and then transferred to the top layer of keratinocytes. Regardless of the color of the skin, the amount of melanocytes is almost constant. However, the difference in the amount, type, and distribution of melanin results in different skin colors.

In human skin, tyrosine is converted to dihydroxyphenylalanine (DOPA) by an enzyme called tyrosinase, and the black-brown polymer melanin is finally formed by a series of oxidation processes.

Since melanin production is an oxidation process, the oxidation process can be accelerated by an oxygen-promoting substance naturally occurring in the human body or by using UV, such as a reactive oxygen species. Therefore, antioxidants (vitamin A/E, flavonoids, tannins, etc.) can delay excessive melanin production, but it does not directly inhibit melanogenesis. In addition, melanin production via melanin at the bottom of the skin is believed to be accelerated by irritation such as UV, burning, and the like. Thus, melanin production can be reduced by reducing external stimuli, blocking signal transfer, or inhibiting the synthesis or activity of melanin producing enzyme tyrosinase.

So far, kojic acid, hydroquinone, arbutin, azelaic acid, aloesin, 4-butyl resorcinol 4-butylresorcinol), resveratrol, ceramide, sphingosine-1-phosphate, sphingosylphosphorylcholine, etc. It is known that melanin production can be regulated by promoting tyrosinase degradation or modulating glycosylation. However, these substances are not widely used because of the lack of good skin whitening effect, low stability, and skin irritation. Therefore, there is a need to develop a substance which can provide a superior skin whitening effect without side effects.

Related technical reference

Patent literature

KR10-1111533 B1.

technical problem

The present invention is to provide a composition which exhibits an excellent skin whitening effect.

Technical solution

In one aspect, the invention provides a composition for skin whitening comprising a gooseberry extract or glutathione.

Beneficial effect

Since the skin whitening composition for use in the present invention exhibits a skin whitening effect by reducing melanin production and inhibiting tyrosinase activity, and has stability without adverse effects of cells, it has been widely used as a composition for external use on skin, Cosmetic compositions and pharmaceutical compositions.

In the following, the invention will be described in detail by way of exemplary embodiments of the invention.

In one aspect, the present invention relates to a composition comprising one or more of gooseberry extract and glutathione as an active ingredient.

In one aspect of the invention, the composition can be a composition for skin whitening.

In one aspect of the invention, the composition according to the invention may be a pharmaceutical, food or cosmetic composition. Further, in one aspect of the present invention, the composition according to the present invention may be a composition for external use on the skin.

In one aspect of the invention, the pharmaceutical or food composition can be an oral formulation.

In one aspect of the invention, the pharmaceutical composition may be selected from one or more of the group consisting of powders, granules, lozenges, soft or hard capsules, suspensions, emulsions, syrups and aerosols.

In one aspect of the invention, the food composition can be selected from one or more of the group consisting of lozenges, granules, powders, beverages, caramel, gels, and cosmetic creams.

In one aspect, the invention relates to a method for skin whitening, the method comprising the steps of administering a currant extract and one or more glutathione; or administering a composition to a skin whitening An entity, wherein the composition comprises one or more of gooseberry extract and glutathione as active ingredients. In one aspect of the invention, the administration can be carried out in accordance with the methods and dosages described herein.

In one aspect, the present invention relates to a composition for skin whitening comprising one or more of gooseberry extract and glutathione as an active ingredient.

In one aspect, the invention relates to the use of one or more of gooseberry extract and glutathione for the manufacture of a composition for skin whitening.

In one aspect, the present invention relates to a composition for reducing melanin comprising one or more of gooseberry extract and glutathione as active ingredients.

In one aspect, the present invention relates to a composition for inhibiting melanin production comprising one or more of gooseberry extract and glutathione as active ingredients.

In one aspect, the invention relates to a method for reducing melanin or inhibiting melanin production, the method comprising the step of administering a currant extract and one or more glutathione; or, administering a combination The substance is required to reduce melanin or inhibit melanin production, wherein the composition contains one or more of gooseberry extract and glutathione as active ingredients. In one aspect of the invention, the administration can be carried out in accordance with the methods and dosages described herein.

In one aspect, the present invention relates to a composition for reducing melanin or inhibiting melanin production, which comprises as an active ingredient one or more of gooseberry extract and glutathione.

In one aspect, the invention relates to one or more of gooseberry extraction and glutathione for use in the manufacture of a composition for reducing melanin or inhibiting melanin production.

In the present invention, "reducing melanin" means reducing melanin of an existing cell or the like, and "inhibiting melanin production" means inhibiting new melanin production of cells and the like.

In one aspect, the present invention relates to a composition for reducing or inhibiting tyrosinase activity, which comprises as an active ingredient one or more of gooseberry extract and glutathione.

In one aspect, the invention relates to a method for reducing or inhibiting tyrosinase activity, the method comprising the steps of administering a currant extract and one or more glutathione; or, administering a The composition provides an entity in need of reducing or inhibiting tyrosinase activity, wherein the composition comprises one or more of gooseberry extract and glutathione as active ingredients. In one aspect of the invention, the administration can be carried out in accordance with the methods and dosages described herein.

In one aspect, the present invention relates to a composition for reducing or inhibiting tyrosinase activity, which comprises as an active ingredient one or more of gooseberry extract and glutathione.

In one aspect, the invention relates to the use of one or more of currant extract and glutathione to produce a composition for reducing or inhibiting tyrosinase activity.

In one aspect, the present invention relates to a composition for inhibiting coloration, which comprises one or more of gooseberry extract and glutathione as active ingredients.

In one aspect, the invention relates to a method for inhibiting pigmentation, the method comprising the steps of administering a currant extract and one or more glutathione; or administering a composition to inhibit An entity of a stain, wherein the composition comprises one or more of gooseberry extract and glutathione as active ingredients. In one aspect of the invention, the administration can be carried out in accordance with the methods and dosages described herein.

In one aspect, the present invention relates to a composition for inhibiting coloration, which comprises one or more of gooseberry extract and glutathione as active ingredients.

In one aspect, the invention relates to the use of one or more of gooseberry extract and glutathione for the manufacture of a composition for inhibiting pigmentation.

In one aspect, the present invention relates to a composition for treating, ameliorating or preventing a stain comprising one or more of gooseberry extract and glutathione as an active ingredient. In particular, the present invention relates to a pharmaceutical composition for treating or preventing pigmentation, or a food composition for improving or preventing color spots, which comprises as an active ingredient a gooseberry extract and glutathione One or more.

In one aspect, the invention relates to a method for treating, ameliorating or preventing a stain, the method comprising the step of administering a currant extract and one or more glutathione; or, administering The composition is for an entity in need of treatment, amelioration or prevention of pigmentation, wherein the composition comprises one or more of gooseberry extract and glutathione as active ingredients.

In one aspect, the present invention relates to a composition for treating, ameliorating or preventing a stain comprising one or more of gooseberry extract and glutathione as an active ingredient.

In one aspect, the invention relates to the use of one or more of gooseberry extract and glutathione for the manufacture of a composition for treating, ameliorating or preventing stains.

In one aspect of the invention, the stain is used from a broad concept, including any stains caused by various causes. Specifically, it means that the skin has a stain, but is not limited thereto. Specifically, in one aspect of the present invention, the pigmentation may include senile keratosis, inflammatory stains, freckles, acquired dermal melanocyte hyperplasia, hyperpigmentation, and the like, but is not limited thereto. .

In the present invention, "pigment" or "hypigmentation" means actual or perceived excessive dark skin damage. Skin damage can be a disease or condition that is excessively exposed to sunlight or causes a dark complexion (ie, due to age). Dark skin may appear spots, multiple spots, or a relatively large dark area. Skin damage can also be perceived, that is, individual perception, that is, the darkness of the skin of the human body is too dark and the individual has a cosmetic that wants to reduce the dark complexion.

Thus, the compositions of the present invention are useful for treating various skin spots (e.g., melanoma that reduces pigmentation, i.e., dark spots on the face or other parts of the body), or self-whitening skin spots. Usually, dark skin damage is due to the higher degree of melanin. The composition according to the invention can be used to treat hyperpigmentation, i.e. to reduce dark skin tone or prevent hyperpigmentation. That is, it can be used to reduce or eliminate the formation of excessive melanin, so as to eliminate dark skin.

In one aspect of the invention, a composition according to one aspect of the invention may comprise a gooseberry extract and a glutathione in a weight ratio of 1:1-9 to 9:1, in particular a weight ratio of 2:8-8 :2. Specifically, the weight ratio of the gooseberry extract to the glutathione may be X:Y, wherein X or Y is an integer between 1 and 20.

In one aspect of the invention, the composition may comprise one or more of from 0.1 to 5,000 ppm (w/w) of gooseberry extract and glutathione based on the total weight of the composition, although not limited thereto . Specifically, in one aspect of the present invention, the composition comprises a concentration of one or more of gooseberry extract and glutathione of 0.01 ppm (w/w, the same below) or higher, 0.1 ppm, respectively. Or higher, 0.5 ppm or higher, 1.0 ppm or higher, 2.0 ppm or higher, 3.0 ppm or higher, 4.0 ppm or higher, 5.0 ppm or higher, 6.0 ppm or higher, 7.0 ppm or higher High, 8.0 ppm or higher, 9.0 ppm or higher, 10.0 ppm or higher, 11 ppm or higher, 12 ppm or higher, 13 ppm or higher, 14 ppm or higher, 15.0 ppm or higher, 16 ppm or higher, 17 ppm or higher, 18 ppm or higher, 19 ppm or higher, 20 ppm or higher, 21 ppm or higher, 22 ppm or higher, 23 ppm or higher, 24 ppm Or higher, 25 ppm or higher, 26 ppm or higher, 30 ppm or higher, 40 ppm or higher, 50 ppm or higher, 60 ppm or higher, 80 ppm or higher, 100 ppm or higher High, 200 ppm or higher, 500 ppm or higher or 1000 ppm or higher, but not limited to this; and 2000 ppm or lower, 1000 ppm or lower, 500 ppm or lower, 20 ppm or lower , 100 ppm or lower, 80 ppm or lower, 60 ppm or Low, 50 ppm or lower, 40 ppm or lower, 30 ppm or lower, 26 ppm or lower, 25 ppm or lower, 24 ppm or lower, 23 ppm or lower, 22 ppm or lower, 21 ppm or lower, 20 ppm or lower, 19 ppm or lower, 18 ppm or lower, 17 ppm or lower, 16 ppm or lower, 15 ppm or lower, 14 ppm or lower, 13 ppm Or lower, 12 ppm or lower, 11 ppm or lower, 10 ppm or lower, 9 ppm or lower, 8 ppm or lower, 7 ppm or lower, 6 ppm or lower, 5 ppm or more. Low, 4 ppm or lower, 3 ppm or lower, 2 ppm or lower, 1 ppm or lower, 0.5 ppm or lower, or 0.1 ppm or lower, based on the total weight of the composition. The concentration is in ppm (w/w).

In one aspect of the invention, the gooseberry may be one or more of a group consisting of leaves, flowers, stems, fruits, and roots selected from the group consisting of woody currant (Ribes grossularia). Specifically, it may be the fruit of gooseberry.

In one aspect of the invention, the gooseberry extraction can be prepared by a method of preparation comprising (1) an extraction step using water, an organic solvent or a combination thereof to extract the gooseberry.

In one aspect of the invention, the method may further comprise the step of treating the gooseberry prior to step (1). Specifically, the treatment may be drying and pulverizing the gooseberry into a powder. However, it is not limited thereto and may include any treatment that promotes extraction. Specifically, the drying method may be sunlight drying, hot air drying, evaporative drying, spray drying, or freeze drying. More specifically, the drying method may be hot air drying. Otherwise, in one aspect of the invention, the live currants can be extracted without any treatment.

In one aspect of the invention, the method may further comprise a removal step for removing the solvent by distillation after extraction. In particular, the distillation can be vacuum distillation.

In one aspect of the invention, the method may further comprise the step of adding one or more of glycerin and a preservative to the concentrate after distillation.

In one aspect of the invention, the method may further comprise the step of filtering the extraction after the step of removing the solvent or after the step of adding one or more of glycerin and a preservative.

In one aspect of the invention, the gooseberry extract can be an extraction selected from one or more of the group consisting of water, organic solvents, and mixtures thereof. Specifically, in one aspect of the present invention, the organic solvent may be selected from C ₁ -C ₆ lower alcohols, one or more of butylene glycol and propylene glycol. More specifically, the lower alcohol may be ethanol.

In the present invention, "skin" refers to an organ covering the surface of an animal's body, including the epidermis, the dermis, and the subcutaneous tissue. It is used from the broadest concept, including the face or body, scalp and hair.

In the present invention, "Rieves grossularia" refers to the dicotyledon of the genus Saccharum of the Saxifragales (mesh) and the family of the Grossulariaceae . Such plants are commonly found in Europe, West Africa, West Asia, South Asia and Southeast Asia and are used as raw materials in desserts such as pies. It is used at the same time as a spice for cooking or as a beverage and is usually supplied as a jam or a dried pulp.

In the present invention, "extraction" includes any substance extracted from a natural product, regardless of the extraction method, extraction solvent, extraction component or extraction type. It is used from a broad concept, including the use of any material obtained by further processing or treatment of substances extracted from natural products. In particular, the treatment or treatment may be an extraction of further fermentation or enzymatic treatment. Thus, the term "extraction" is used in the broad sense of the invention to include fermentation products, concentrated and dried products. Specifically, the extraction may be the fermentation product of the present invention.

In the present invention, glutathione refers to a compound of the following Chemical Formula 1, which has a CAS number of 70-18-8 and a molecular weight of 307.32.

Chemical formula 1

In the present invention, "currant extract" includes any substance extracted from gooseberry, regardless of the extraction method, extraction solvent, extraction component or extraction type. It is used from a broad concept, including substances extracted from gooseberry by treatment with heat, acid, alkali, enzymes, etc.; and any substance obtained by further treatment or disposal of the extracted material. In particular, the treatment or treatment may be further fermented or enzymatically treated with gooseberry extraction. Therefore, the gooseberry extract of the present invention may be a fermentation product.

In one aspect of the present invention, "Rieves grossularia" may be extracted, live currants, smashed live currants, dried sorrel, dried smash of live gooseberry or The form of the fermented product of gooseberry is not limited to this. The gooseberry used in the present invention does not limit how it is obtained. It can be cultivated or purchased commercially. In addition, all or part of the aerial part or root may be used. More specifically, one or more selected from the group consisting of leaves, flowers, stems, fruits and roots of woody plant gooseberry can be used. The gooseberry of the present invention does not necessarily have to be dried, and may be in any form as long as it is suitable for extracting the active ingredient of the gooseberry.

In one aspect of the invention, the water comprises distilled water or pure water, and the organic solvent comprises one or more selected from the group consisting of alcohols, for example, C ₁ -C ₅ lower alcohols, acetone, diethyl ether, ethyl acetate, diethyl ether. Methyl ethyl ketone and chloroform, but are not limited thereto.

In one aspect of the present invention may comprise extraction gooseberry gooseberry extract C ₁ -C ₆ alcohols. Specifically, the alcohol may be methanol or ethanol.

In one aspect of the invention, the gooseberry extraction can be obtained by a method of preparation, including an extraction step using water, an organic solvent or a mixture thereof to extract the gooseberry.

In one aspect of the invention, the gooseberry extract can be a crude extraction of a solvent selected from the group consisting of water, organic solvents, and combinations thereof. The organic solvent may be a C ₁ -C ₆ alcohol. Specifically, the C ₁ -C ₆ alcohol may be methanol or ethanol. In one aspect of the present invention, the gooseberry can be extracted using a solvent which is 5-15 times (more specifically about 10 times) in accordance with about gooseberry, but is not limited thereto.

In one aspect of the invention, the extraction can be carried out using hot water extraction, ethanol extraction, heat extraction, cryoprecipitation extraction, reflux extraction, reflux condensation extraction, ultrasonic extraction, and the like. Those skilled in the art should understand that the extraction method is not limited. Specifically, the extraction can be carried out by hot water extraction or ethanol extraction.

In one aspect of the invention, the extraction can be carried out at room temperature. However, for more efficient extraction, it can be carried out at a relatively high temperature of about 40-100 ° C (more specifically about 65-75 ° C), but is not limited thereto. The extraction time may be from about 2 to 48 hours, especially from 18 to 36 hours, more specifically from 20 to 28 hours, and most specifically from 22 to 26 hours, although it is not limited thereto. The extraction time may vary depending on the conditions of the extraction solvent, the extraction temperature, and the like. The extraction can be carried out one or several times to obtain a large amount of the active ingredient. Specifically, the extraction can be carried out continuously for 1-5 times, more specifically 3 times, and the obtained extracts can be combined.

In one aspect of the invention, the gooseberry extract may comprise a crude extraction of the gooseberry, as described above, and may also include a soluble component obtained by further extracting the crude extract using a low polarity organic solvent. In one aspect of the invention, the organic solvent may be n-hexane, dichloromethane, ethyl acetate, n-butanol, or the like, but is not limited thereto. The extracted or extracted soluble component obtained as described above may be used after filtration and concentration, or after concentration and drying.

In one aspect of the invention, the drying treatment can be dry evaporation, spray drying or freeze drying. Specifically, freeze-drying may be performed for 3-4 days at -50 to -70 ^o C.

The formulation of the cosmetic composition according to one aspect of the present invention is not particularly limited and may be appropriately selected depending on the purpose. For example, it can be prepared as one or more preparations selected from the group consisting of body lotions, skin softeners, skin toners, astringents, lotions, lotions, moisturizing lotions, nourishing lotions, massage creams, nourishing creams , moisturizing cream, hand cream, foundation, perfume, nourishing beauty lotion, moisturizing mask, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser, but not limited to this.

When the preparation of the cosmetic composition according to one aspect of the present invention is a paste, a cream or a gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol (polyethylene) Glycol), organic hydrazine, bentonite, cerium oxide, talc, zinc oxide, etc. can be used as a vehicle.

When the preparation of the cosmetic composition according to one aspect of the present invention is a powder or a spray, lactose, talc, vermiculite, aluminum hydroxide, calcium citrate, polyamide powder can be used as a vehicle. In particular, the spray may further comprise a propellant such as chlorofluorocarbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition according to one aspect of the present invention is a solution or emulsion, the solvent, solubilizer or emulsifier can be used as a vehicle. Examples include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 1,3-butylene glycol, a glycerol aliphatic ester, polyethylene glycol or a fatty acid ester of sorbitan.

When the cosmetic composition according to one aspect of the present invention is formulated as a suspension, a liquid diluent such as water, ethanol or propylene glycol, or a suspending agent such as ethoxylated isostearyl alcohol , polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite ), agar, tragacanth, etc. can be used as a vehicle.

When the preparation of the cosmetic composition according to one aspect of the present invention is a surfactant-containing detergent, an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfo amber Sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Fatty acid amide ether sulfate, alkyl amidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, A lanolin derivative, an ethoxylated glycerol fatty acid ester, or the like can be used as a vehicle.

The cosmetic composition according to one aspect of the present invention includes, in addition to the gooseberry extract or glutathione, functional additives and ingredients generally included in the cosmetic composition. The functional additive may comprise a component selected from the group consisting of water soluble vitamins, oil soluble vitamins, polypeptides, polysaccharides, sphingolipids, and seaweed extracts.

If desired, the cosmetic composition according to an aspect of the present invention may further comprise a component and a machine work additive which are usually included in the cosmetic composition. This ingredient may include oils, fats, humectants, emollients, surfactants, organic or inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH control agents, ethanol, dyeing Agents, fragrances, blood circulation irritants, coolants, antiperspirants, pure water, and the like.

In another aspect, the present invention relates to a preparation for external use on the skin, which comprises one or more of gooseberry extract, glutathione as an active ingredient. The preparation for external use on the skin includes any type of preparation which can be applied from the outside of the skin, and various types of cosmetic preparations can be included.

Pharmaceutical compositions according to one aspect of the invention may be prepared in a variety of oral or parenteral formulations. The preparation is prepared by using a commonly used diluent or excipient such as a filler, a bulking agent, a binder, a moisturizer, a disintegrant, a surfactant, and the like. Solid preparations for oral administration include lozenges, pills, powders, granules, soft or hard capsules, and the like. The solid preparation is prepared by mixing the active ingredient with at least one excipient (for example, starch, calcium carbonate, sucrose, lactose, gelatin, and the like). Lubricants such as magnesium stearate, talc, and the like can be used in addition to simple excipients. Liquid preparations for oral administration include suspensions, internal liquids, emulsions, syrups, and the like. In addition to the simple diluents commonly used, such as water and liquid paraffin, various excipients (eg, wetting agents, sweeteners, aromatics, preservatives, and the like) can be included. Formulations for parenteral administration include bactericidal aqueous solutions, nonaqueous solutions, suspensions, emulsions, lyophilized formulations, and suppositories. For non-aqueous solutions or suspensions, propylene glycol, polyethylene glycol, vegetable oils (such as olive oil), injectable esters (such as ethyl oleate, and the like) can be used as a solvent. As the suppository base, glycerol trilaurate, polyethylene glycol, Tween 61, cocoa butter, lauric fat, glycerin gelatin, and the like can be used.

The active ingredient of the composition according to one aspect of the invention may be used in the form of a pharmaceutically acceptable salt. In addition, it can be used alone in combination with other pharmaceutically active compounds. There is no particular restriction as long as the salt is pharmaceutically acceptable. For example, hydrochloride, sulfate, nitrate, phosphate, hydrofluoride, hydrobromide, formic acid, acetate (acetate), tartrate, lactate, citrate, fumarate, maleate, succinate, methanesulfonate (methanesulfonate), benzenesulfonate, toluenesulfonate, naphthalenesulfonate, and the like can also be used.

Compositions according to one aspect of the invention may be administered parenterally or orally with the purpose. A daily dose of 0.1-500 mg (mg) per kilogram of body weight (especially 1-100 mg (mg)) can be administered in one dose or divided into many smaller doses. The dosage of a particular patient can vary depending on the patient's weight, age and sex, physical condition, diet, time of administration of the drug, method of administration of the drug, rate of excretion, severity of the disease, or the like.

The pharmaceutical composition according to one aspect of the present invention can be prepared into any type of suitable preparation according to a commonly used method, including oral preparations (such as powders, granules, troches, soft or hard capsules, suspensions, emulsions, A syrup, a drink, an aerosol, etc.), a skin external preparation (such as an ointment, a cream, and the like), a suppository, an injection, a sterile injection, and the like. Specifically, it can be prepared as an injection or preparation for external use on the skin.

Compositions according to one aspect of the invention can be administered to mammals, such as rats, mice, domestic animals, humans, and the like, by a variety of routes, including parenteral and oral routes. Any mode of investment can be expected. For example, the composition can be administered orally, transdermally, rectally, intravenously, intramuscularly, subcutaneously, intrauterinely or intracerebrovascularly.

Compositions according to one aspect of the present invention can be administered by a variety of routes that are readily accepted by those skilled in the art. In particular, the pharmaceutical composition according to the present invention can be administered to the surface of the skin like a preparation for external use on the skin.

In one aspect of the invention, the food composition can be a health functional food composition.

The preparation of the food composition according to one aspect of the present invention is not particularly limited. For example, the formulation can be prepared into tablets, granules, powders, liquids (such as beverages, caramels, gels, cosmetic creams, and the like). Each of the preparations of the food composition may contain the active ingredient; and the commonly used ingredients, which are readily selected by the skilled artisan, depending on the type of preparation and the purpose of use. Synergism can be formed when the active ingredient is used in conjunction with other active ingredients.

In the food composition according to one aspect of the present invention, it is within the scope of the skilled artisan to determine the dosage of the active ingredient to be administered. For example, a daily dose of 0.1-5000 mg/kg/day (mg/kg per day), more system, 50-500 mg/kg/day (mg/kg per day), but not limited to this . The dosage administered can vary with various factors, such as the age of the entity, physical condition, the presence of complications, or other similar conditions.

For example, a food composition according to an aspect of the present invention may be a food (such as chewing gum, caramel, candy, frozen confection, candy, etc.), a beverage (such as a soft drink, mineral water, alcoholic beverage, etc.). , or healthy functional foods (such as vitamins, minerals, etc.).

In addition, the food composition according to an aspect of the present invention may further comprise nutrients, vitamins, minerals (electrolytes), synthetic or natural flavors, coloring agents, extenders (cheese, chocolate, etc.), pectic acid ( Or a salt thereof, alginic acid (or a salt thereof), an organic acid, a protective colloid thickener, a pH controlling agent, a stabilizer, a preservative, glycerin, ethanol, a foaming agent used in a soft drink, and the like. Moreover, the functional food composition according to an aspect of the present invention may comprise pulp for preparing a natural juice, a fruit juice drink or a vegetable juice. These ingredients may be used singly or in combination. The ingredients of these additives are not of significant importance, but are typically from 0 to 20% by weight of the total (per 100%) by weight of the composition of the invention.

Hereinafter, the present invention will be described in detail through examples and test examples. However, the following examples and test examples are for illustrative purposes only, and the scope of the invention is not limited thereto.

Example 1 - Preparation of Gooseberry Fruit Extract

The fruit of the gooseberry is harvested first, dried by hot air, and then pulverized into a powder. The powder was hot water extracted at 70 ° C using an ethanol/water mixture for 24 hours, and then the ethanol was removed by vacuum distillation. Gooseberry fruit extract is obtained by adding glycerin and a preservative to the concentrate and then filtering. The gooseberry fruit extract prepared as described above was purchased from Stauber (USA) for use in the following experiment.

Test Example 1 - Determination of melanin

(1) Treatment by extraction and color observation

MNT-1 cells (human melanoma cell line of Lonza, Switzerland) showing excessive melanin production were purchased and inoculated into 100-π culture dishes. The cells were treated with no additives (control), gooseberry fruit extraction of Example 1, glutathione (Sigma-Aldrich, PHR 1359, hereinafter the same) or a combination of two specific weight ratios. The test group used 10 ppm (w/w) and 20 ppm (w/w) gooseberry fruit extract and glutathione, or 10 ppm currant fruit extract at weight ratios of 2:8, 5:5 and 8:2, respectively. Treated with a mixture of glutathione. After four days of treatment (when the vehicle was exchanged and sampled every other day), the cell lysate was reduced by centrifugation (four days using sample treated cells using 200 mL (microliter) RIPA (radioimmunoprecipitation assay) buffer, And centrifugation at 13,000 rpm for 15 minutes; Centrifuge 5415R centrifuge of Eppendorf, Germany), while the precipitate was separated and observed for color.

The results are shown in Figure 1. As shown in Figure 1, the test group treated with 20 ppm gooseberry fruit extract or glutathione showed a brighter color as the control group appeared black. In particular, it can be seen that the test group treated with the gooseberry fruit extract and the glutathione mixture showed a brighter color. Although the concentration of the mixture is only half (10 ppm), the effect is more significant. Therefore, it was confirmed that the currant fruit extract and glutathione of the present invention exhibited an effect of reducing cell melanin, and the effect was more remarkable when both gooseberry fruit extract and glutathione were used. Therefore, it was confirmed that the present invention can provide an effect of whitening the skin or treating the stain.

(2) Determination of protein and melanin amount

200 mL (microliter) of RIPA buffer was added to MNT-1 cells cultured in 6-well plates (human melanoma cell line in Longsha, Switzerland). After stirring for 1 minute, the cells were reconstituted. Then, after centrifugation at 13,000 rpm for 15 minutes, the supernatant was separated from the cell pellet.

1 mL (microliter) of the supernatant was added to a 96-well plate, and then distilled water was added to make a final volume of 25 mL (microliter). Then, the amount of protein was measured at 562 nm (nano) using a BCA protein quantification reagent set (Thermo Scientific, Illinois, USA). The results are shown in Table 1.

Table 1

Add 200 mL (microliter) of 1N sodium hydroxide (NaOH) (Sigma-Aldrich, St. Louis, USA) to the precipitate in (1) above and dissolve at 60 °C for 15 minutes, then dissolve the precipitate by 50 mL (microliter). The absorbance was measured at 490 nm (nano) on a 96-well plate (respectively repeated 3 times). The amount of melanin is calculated from Equation 1 based on the determined amount of protein. The results are shown in Figure 2.

Equation 1

Melanin amount (relative to control) = {(in 490 nm (nano) sample absorbance) / (control absorbance)} × {(sample protein amount) / (control protein amount)}

As shown in Figure 2, 20 ppm gooseberry fruit extract and glutathione exhibited 21.1% and 19.4% melanin production inhibition, respectively. When the cells were treated with a combination of gooseberry fruit extract and glutathione, the melanin production inhibitory effect was 46.9% at a weight ratio of 2:8, 62.3% at a weight ratio of 5:5, and 37.9% at a weight ratio of 8:2. . Therefore, as shown in Fig. 1, it was also confirmed that the gooseberry extract and glutathione each inhibited melanin production and provided skin whitening and treatment of pigmentation. When two substances are used at the same time, the effect is very significant. When it is used at the same time, if the weight ratio is 5:5, the melanin production inhibition effect is the highest.

Test Example 2 - Inhibition of tyrosinase activity

Based on the amount of protein determined in (2) of Test Example 1, 20 mg (microgram) of cell supernatant contained protein protein added to a 96-well plate, and PBS (Welgene's phosphate buffered saline, not Calcium chloride (CaCl ₂ ) & magnesium chloride (MgCl ₂ )) produced a final volume of 100 mL (microliters). Then, 100 mL (microliter) of 2 mg/mL (mg/ml) of L-DOP (L-3,4-dihydroxybenzene) (Sigma-Aldrich, St. Louis, USA) in PBS was further added. The dopachrome production from L-DOPA by the action of tyrosinase was compared by measuring the absorbance. Although cultured at 37 °C after the addition of L-DOPA, the absorbance was measured at 490 nm (nano) using a 10-minute interval. The result of calculating tyrosinase activity according to Equation 2 of the amount of protein determined in (2) of Test Example 1 is shown in Fig. 3.

Equation 2

Tyrosinase activity (relative to control) = (490 nm (nano) sample absorbance) / (control absorbance)

As shown in Figure 3, tyrosinase activity was inhibited at 80% and 78% (relative to the control group) when cells were treated with 20 ppm gooseberry fruit extract and glutathione, respectively. When both gooseberry fruit extract and glutathione are used, the tyrosinase activity inhibitory effect is significantly increased (when compared to its use alone). Specifically, when the weight ratio is 2:8, the tyrosinase activity is inhibited at 38% (relative to the control group), and at a weight ratio of 5:5, the activity is inhibited at 67%, and At a weight ratio of 8:2, its activity is inhibited at 60%.

From the results of Test Examples 1 and 2, it was confirmed that the gooseberry fruit extract and glutathione according to one aspect of the present invention are effective for inhibiting melanin production and reducing tyrosinase activity. In particular, the effect is significant when used alone when compared to when it is used alone (even when the concentration is only half). Therefore, it was confirmed that when two substances were used at the same time, a remarkable synergistic effect of suppressing melanin production and reducing tyrosinase activity can be achieved. Therefore, the composition according to an aspect of the present invention will exhibit a remarkable effect of whitening the skin, inhibiting melanin production, reducing (inhibiting) tyrosinase activity, or inhibiting the pigment.

Hereinafter, the present invention will be described in detail through a formulation example. However, the following formulation examples are for illustrative purposes only, and the scope of the invention is not limited thereto.

Formulation Example 1 - Soft Capsules

The soft capsule filling solution was obtained by extracting 8 mg (mg) of the currant fruit of Example 1 according to a commonly used method and glutathione, 9 mg (mg) of vitamin E, 9 mg (mg) of vitamin C, 2 mg (mg Prepared by mixing palm oil, 8 mg (mg) hydrogenated vegetable oil, 4 mg (mg) yellow beeswax and 9 mg (mg) lecithin. Soft capsules were prepared by filling 400 mg (mg) of solvent per capsule. Further, the soft capsule skin is prepared by using 66% gelatin weight, 24% glycerin weight and 10% sorbitol liquid weight, and the soft capsule containing the 400 mg (mg) composition according to the present invention is filled with a filling solvent. Prepared by capsule skin.

Formulation Example 2 - Lozenges

8 mg (mg) Example 1 of gooseberry fruit extract and glutathione, 9 mg (mg) vitamin E, 9 mg (mg) vitamin C, 200 mg (mg) galactooligosaccharide, 60 mg (mg) lactose It was mixed with 140 mg (milligrams) of maltose and granulated using a fluid bed dryer. After the addition of 6 mg (mg) of the sugar ester, the 500 mg (mg) of the resulting composition was prepared into a tablet according to a commonly used method.

Formulation Example 3 - Beverage

8 mg (mg) Example 1 of gooseberry fruit extract and glutathione, 9 mg (mg) of vitamin E, 9 mg (mg) of vitamin C, 10 g (g) of glucose, 0.6 g (g) of citric acid and 25 g (g) oligosaccharide syrup is mixed. After adding 300 mL (ml) of pure water, each bottle was filled with 200 mL (ml) of the mixture. The beverage is then sterilized at 130oC for 4-5 seconds.

Formulation Example 4 - Granules

8 mg (mg) Example 1 of gooseberry fruit extract and glutathione, 9 mg (mg) of vitamin E, 9 mg (mg) of vitamin C, 250 mg (mg) of anhydrous crystalline glucose and 550 mg (mg) of starch Mix and granulate using a fluid bed granulator. The prepared granules are filled in a pouch.

Formulation Example 5 - Injection

Injections were prepared according to the commonly used method using the compositions described in Table 2.

Table 2

Formulation Example 6 - Health Function Food

Health functional foods were prepared according to the commonly used method using the compositions described in Table 3.

table 3

While the aforementioned vitamin and mineral ingredients are presented in a particular instance that is quite suitable for a healthy functional food, it may vary as needed.

Formulation Example 7 - Healthy Drinks

Healthy beverages were prepared according to the commonly used method using the compositions described in Table 4.

Table 4

According to a commonly used method for preparing a healthy beverage, the above ingredients are mixed and heated at 85 ° C for about 1 hour while stirring. The prepared solution is filtered and sterilized.

Formulation Example 8 - Softening lotion (emollient)

The skin lotion was prepared according to the commonly used method using the compositions described in Table 5.

table 5

Formulation Example 9 - Moisturizing Emulsion (Milk Emulsion)

Moisturizing emulsions were prepared according to the commonly used method using the compositions described in Table 6.

Table 6

Formulation Example 10 - Nourishing Cream

The nourishing cream was prepared according to the commonly used method using the compositions described in Table 7.

Table 7

Formulation Example 11 - Massage Cream

The massage cream was prepared according to the commonly used method using the composition described in Table 8.

Table 8

Formulation Example 12 - Mask

The mask was prepared according to the commonly used method using the compositions described in Table 9.

Table 9

While the invention has been shown and described with reference to the embodiments of the embodiments

Figure 1 shows the results of treatment of MNT-1 cells with gooseberry extract or glutathione and comparison of color.

Figure 2 shows the results of treatment of MNT-1 cells with gooseberry extract or glutathione and determination of the amount of melanin.

Figure 3 shows the results of treatment of MNT-1 cells with gooseberry extract or glutathione and determination of tyrosinase activity.

Claims (17)

A composition for external use on the skin, which comprises as an active ingredient one or more of gooseberry extract and glutathione. A composition for skin whitening comprising one or more of gooseberry extract and glutathione as an active ingredient. A composition for inhibiting melanin production or reducing melanin, which comprises one or more of gooseberry extract and glutathione as an active ingredient. A composition for inhibiting a pigment comprising one or more of gooseberry extract and glutathione as an active ingredient. The composition of any one of claims 1 to 4, wherein the composition comprises as an active ingredient a gooseberry extract and glutathione. The composition of claim 5, wherein the composition comprises the gooseberry extract and glutathione in a weight ratio of 1:9 to 9:1. The composition according to any one of claims 1 to 4, wherein the gooseberry is one or more selected from the group consisting of leaves, flowers, stems, fruits and roots of woody plant gooseberry. The composition of claim 7, wherein the gooseberry is the fruit of a woody plant gooseberry. The composition of any one of claims 1 to 4, wherein the composition comprises 1 ppm (w/w) or more of gooseberry extract or glutathione based on the total weight of the composition. The composition of any one of claims 1 to 4, wherein the gooseberry extract is an extract selected from one or more of the group consisting of water, an organic solvent, and a mixture thereof. The composition according to the requested item 10, wherein the organic solvent is selected from C ₁ -C ₆ lower alcohols, butylene glycol and propylene glycol, one or more of the group. The composition of claim 11 wherein the lower alcohol is ethanol. The composition of claim 1 wherein the composition is a pharmaceutical or cosmetic composition. The composition of any one of claims 2 to 4, wherein the composition is a pharmaceutical, food or cosmetic composition. The composition of claim 14, wherein the pharmaceutical or food composition is an oral preparation. The composition of claim 15, wherein the pharmaceutical composition is one or more selected from the group consisting of a powder, a granule, a lozenge, a soft or hard capsule, a suspension, an emulsion, a syrup, and an aerosol. The composition of claim 15 wherein the food composition is selected from the group consisting of a tablet, a granule, a powder, a beverage, a caramel, a gel, and a cosmetic cream.