KR102680423B1 - A plant extract using high frequency, and a functional cosmetic composition containing thereof - Google Patents

Abstract

The present invention relates to a high-frequency plant extract and a functional cosmetic composition containing the same. More specifically, a plant extract using high-frequency waves and the plant extract containing the same as an active ingredient provide anti-oxidation, anti-inflammatory, whitening improvement, photo-aging inhibition, moisturizing improvement, and/ Or it relates to a functional cosmetic composition for suppressing wrinkles. The extract containing pansy, gooseberry, and dogwood in combination of the present invention has excellent skin antioxidant, anti-inflammatory, whitening improvement, photoaging suppression, moisturizing, and/or anti-wrinkle effects, and is therefore expected to be widely used in the beauty field. .

Description

Plant high frequency extract, and functional cosmetic composition containing the same {A plant extract using high frequency, and a functional cosmetic composition containing the same}

The present invention relates to a high-frequency plant extract and a functional cosmetic composition containing the same. More specifically, a plant extract using high-frequency waves and the plant extract containing the same as an active ingredient provide anti-oxidation, anti-inflammatory, whitening improvement, photo-aging inhibition, moisturizing improvement, and/ Or it relates to a functional cosmetic composition for suppressing wrinkles.

In modern times, with the development of industrial society and the increase in social activities, self-consciousness to actively express oneself is increasing, and the desire to use cosmetics to make one's skin beautiful and keep it healthy is greatly increasing. Cosmetic compositions with various ingredients are being developed to meet needs. However, since most compositions involve the discovery or combination of new chemicals, they have the problem of acting as an irritant to sensitive skin. As an example, hydroxy acids and retinoids have been proven to provide benefits such as improvements in the appearance, skin radiance, and treatment of age spots in photodamaged or naturally aged skin, but when applied in high concentrations, these ingredients They are sometimes known to have side effects such as skin irritation, such as skin redness and stinging sensation upon application. Therefore, recent efforts have continued to develop functional skin cosmetic compositions using natural plant extracts that are safe from skin irritation.

On the other hand, the useful components of plants will be very different depending on the extraction method, and their efficacy will be very different, but for skin improvement effects such as skin antioxidant, anti-inflammatory, whitening improvement, photoaging suppression, moisturizing improvement, and/or wrinkle suppression, etc. Research on extraction methods for plant useful components is insufficient.

Against this background, the present inventors made diligent efforts to develop a cosmetic composition based on plant extracts that is safe for the skin and has excellent skin antioxidant, anti-inflammatory, whitening improvement, photoaging inhibition, moisturizing improvement, and/or anti-wrinkle effects, and thus completed the present invention. . The extract containing pansy, gooseberry, and dogwood in combination of the present invention has excellent skin antioxidant, anti-inflammatory, whitening improvement, photoaging suppression, moisturizing, and/or anti-wrinkle effects, and is therefore expected to be widely used in the beauty field. .

The present invention was developed in response to the above-mentioned needs, and is a functional cosmetic composition containing skin antioxidant, anti-inflammatory, whitening improvement, photoaging suppression, moisturizing improvement, and/or anti-wrinkle functional cosmetic composition mainly containing complex extracts of pansy, gooseberry, and dogwood, and It relates to its manufacturing method.

Therefore, the purpose of the present invention is to provide a functional cosmetic composition containing skin antioxidant, anti-inflammatory, whitening improvement, photoaging suppression, moisturizing improvement, and/or wrinkle suppression, and a method for producing the same, which contains complex extracts of pansy, gooseberry, and dogwood as main ingredients. Do it as

Another object of the present invention is to provide a functional food composition containing skin antioxidant, anti-inflammatory, whitening improvement, photoaging suppression, moisturizing improvement, and/or wrinkle suppression, and a method for producing the same, which mainly contains complex extracts of pansy, gooseberry, and dogwood. Do it as

Another object of the present invention is to provide a pharmaceutical composition for the treatment of inflammation, freckles, freckles, or age spots, which contains a complex extract of pansy, gooseberry, and dogwood as a main ingredient, and a method for producing the same.

However, the technical problem to be achieved by the present invention is not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below.

DETAILED DESCRIPTION OF THE INVENTION Various embodiments described herein are described below with reference to the drawings. In the following description, various specific details, such as specific forms, compositions, and processes, are set forth in order to provide a thorough understanding of the invention. However, certain embodiments may be practiced without one or more of these specific details or in conjunction with other known methods and forms. In other instances, well-known processes and manufacturing techniques are not described in specific detail so as not to unnecessarily obscure the invention. Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, form, composition or characteristic described in connection with the embodiment is included in one or more embodiments of the invention. Accordingly, the phrases “in one embodiment” or “an embodiment” expressed in various places throughout this specification do not necessarily refer to the same embodiment of the invention. Additionally, particular features, shapes, compositions, or properties may be combined in any suitable way in one or more embodiments.

Unless there is a special definition in the specification, all scientific and technical terms used in the specification have the same meaning as commonly understood by a person skilled in the art in the technical field to which the present invention pertains.

In one embodiment of the present invention, “Blackberry lily” is a perennial herb of the monocotyledonous family Iris. It grows in mountainous areas and along the seashore and is 50 to 100 cm tall. The rhizome is cut short to the side, the stem stands straight, and branches grow from the upper part. The leaves are alternate, sword-shaped, flat on the left and right, and lined up in two rows. The color is slightly white on a green background, and the base surrounds the stem. The leaves are 30 to 50 cm long and 2 to 4 cm wide. Flowers bloom from July to August, are 5 to 6 cm in diameter, spread horizontally, and have dark spots on a yellowish red background. The ends of the branches are split once or twice, several flowers grow in one place, and there are 4 to 5 bracts at the bottom. There are 6 perianth pieces and they are oval shaped. There are three stamens and the ovary is of the lower order. The style stands straight and is divided into three branches. The fruit is a capsule, shaped like an upside-down egg, about 3cm long, and ripens in September to October. The seeds are ball-shaped, black, and shiny. It is cultivated for ornamental purposes and is distributed in Korea, Japan, and China.

In one embodiment of the present invention, “Gooseberry” is a thorny deciduous shrub (Ribes grossularia) of the Saxiragaceae family. The fruit is a round berry of green, yellow, or red color with a thin, transparent, hairy skin. It has a sweet and sour taste and is eaten raw or used for jam, jelly, and syrup. It is native to Europe, North Africa, and Southwest Asia and is cultivated all over the world. There are various varieties depending on the production area, including Hinomaki red gooseberry (Hinnonmaki rot gooseberry), Hinomaki gold gooseberry (Ribes grossularia L gooseberry), Hinnonmaki green gooseberry (Hinnonmaki gelb ISK gooseberry), Invicta gooseberry (Invicta gooseberry), and pixel gooseberry ( There are varieties such as Pixwell Gooseberry, Indian Gooseberry (Phyllanthus emblica), and Captivato (Ribes uva_crispa captivator).

In one embodiment of the present invention, “Cornus controversa” is a deciduous tree of the Dogwood family, an umbel of the dicotyledonous plant Dipanhwa group. It grows in mountain valley forests, reaches a height of 20 m, and has branches that hang in layers and spread horizontally. The small branches turn dark red purple in the winter, and when the branches are cut in the spring, water flows. The leaves are alternate, broadly oval shaped, and have pointed ends. The leaf edges are plain, have 5 to 8 rows of lateral veins, the petiole is red, and the back of the leaf is white. There are fine hairs on both sides of the leaves. The flowers bloom from May to June and are white and form a corymb. The petals are broadly lanceolate and have hairs on the outside along with the sepals. There are 4 stamens, the anthers are T-shaped, and there is 1 pistil. The fruit is a drupe that is round and ripens to purple-black. Distributed in Korea, Japan, and China.

In one embodiment of the present invention, “aerial part” refers to the part of the plant that is exposed above the ground (soil). In general, elements belonging to the above-ground part include stems, leaves, flowers, bark, and fruits, but are not limited thereto.

In one embodiment of the present invention, “underground part” refers to the part below the ground (soil) among the parts that make up the plant. In general, elements belonging to the underground part refer to roots, and the roots include storage roots such as radish, carrot, ginseng, arrowroot, and sweet potato. Even if it exists in the stem or below the ground, such as bamboo shoots, taro, lotus roots, etc., it can also be included in the underground part.

In one embodiment of the present invention, “extract” refers to an active ingredient separated from a natural product, for example, a preparation obtained by squeezing a natural product with an appropriate solvent, filtering it, and then evaporating the solvent to concentrate it. In addition, it may be an extract obtained through extraction treatment, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, or a purified product thereof, but is not limited thereto. The extraction method is not limited thereto, but can be obtained by an extraction method selected from the group consisting of solvent extraction, ultrasonic extraction, supercritical extraction, hot water extraction, high frequency extraction, fermentation, and fermentation methods. In the present invention, hot water extraction, or It is preferable to use a high-frequency extraction method, and it is more preferable to use a high-frequency extraction method. At this time, the high-frequency extraction may be performed at a frequency of 100 to 3000 kHz, 300 to 2000 kHz, or 700 to 1200 kHz, but is not limited thereto, and is preferably performed at a frequency of 800 to 1100 kHz, and is preferably performed at a frequency of 900 to 1100 kHz. More preferably, it is performed at a frequency of from 1000 kHz. Alternatively, the high-frequency extraction may be performed with a power of 100 to 1000 W, 200 to 800 W, 300 to 600 W, or 100 to 400 W, but is not limited thereto, and is preferably performed with a power of 200 to 400 W. . The hot water extraction may be performed at 10 to 85°C, 20 to 75°C, or room temperature for 2 to 96 hours, 6 hours to 48 hours, 12 to 36 hours, or 4 to 12 hours, and the high-frequency extraction may be performed at 20 to 120 °C. It may be performed for 1 to 12 hours, 1 to 8 hours, 2 to 6 hours, or 2 to 4 hours at 30°C to 22°C, or 22 to 26°C, or at room temperature. When using the hot water extraction method or high frequency extraction method, the solvent may be extracted with a solvent selected from the group consisting of water, ethanol, organic solvents, and mixed solvents thereof. In the present invention, purified water, butylene glycol, or a mixed solvent thereof is preferred, but is not limited thereto.

In one embodiment of the present invention, “antioxidation” refers to inhibiting oxidation and is a concept of inhibiting cellular aging. Oxygen that enters the body through breathing creates energy needed by the human body and at the same time creates free radicals, which are harmful excess oxygen. Free radicals attack normal cells in the body and act as a cause of aging or various diseases. Therefore, removing free radicals is a way to prevent oxidation (aging) of cells, and antioxidants are those that inhibit the oxidation of cells.

Representative antioxidant substances include vitamin C (found in large amounts in kiwi, cabbage, etc.), vitamin E (found in large amounts in almonds, sunflower seeds, etc.), beta-carotene (found in large amounts in carrots, tomatoes, etc.), and selenium (found in large amounts in various seafood). there is.

In one embodiment of the present invention, “anti-inflammatory” refers to the effect of alleviating and resisting inflammation. The anti-inflammatory function in skin tissue can be seen as protecting and stabilizing the skin, as well as disinfecting and sterilizing the skin.

In one embodiment of the present invention, “whitening” refers to the function of preventing excessive deposition of melanin pigment on the skin due to the increase in melanin cells due to continuous exposure of the skin to ultraviolet rays, or thinning the existing melanin pigment. It refers to Accordingly, the formation of freckles, freckles, age spots, and melanoma caused by excessive deposition of melanin pigment can be suppressed.

In one embodiment of the present invention, “photoaging” refers to an aging phenomenon caused by sunlight. Among human tissues, the area most frequently exposed to sunlight is the skin, so photoaging can be understood to primarily mean skin aging. Among sunlight, the rays that cause skin damage are ultraviolet rays, and ultraviolet B (280-320 nm), which has a short wavelength, is known to be the main cause of skin damage. When repeatedly exposed to ultraviolet rays for a long time, the skin loses elasticity, develops wrinkles, becomes rough, and shows changes such as senile spots. In photoaged skin, the epidermis thickens, a large amount of abnormal elastin accumulates in the upper layer of the dermis, the amount and structure of dermal collagen are damaged, and mucopolysaccharide increases. In addition, inflammatory reactions occur, such as an increase in mast cells and an increase in the number of white blood cells in the tissue.

In one embodiment of the present invention, “moisturizing” refers to a series of actions that supply moisture into tissue cells, and skin moisturizing means maintaining moisture in the skin to soften the tissue. When there is a lack of moisture in the skin tissue, the skin's protective film is damaged, making it rough and dry. Dry skin promotes wrinkles and aging, so skin moisturization is an important item to maintain healthy skin.

In one embodiment of the present invention, “wrinkles” are a phenomenon in which collagen fibers, elastic fibers, etc. in the dermis are degenerated due to various factors, including sunlight, and skin moisture is reduced, resulting in loss of skin elasticity and folding of the skin. . Absorption of subcutaneous fat also worsens wrinkles. In aging skin, the collagen fibers that make up most of the extracellular matrix deteriorate and changes in skin components such as hyaluronic acid and ceramide cause wrinkles to form, skin moisture loss, dryness, and loss of elasticity. In aged skin, the number of dermal and epidermal cells decreases, and cell function deteriorates, resulting in wrinkles, pigmentation, dryness, thick skin, and decreased elasticity. Exposure to ultraviolet rays induces the production of matrix metalloproteinase (MMP) in the skin, which causes the degradation of extracellular matrix (ECM) proteins such as collagen, elastin, and proteoglycans. Among these, MMP-1 is a collagen-degrading enzyme that decomposes type 1 collagen (COL1A1) and type 3 collagen (COL3A1), the main types of skin. Collagen synthesis has been reported to decrease in naturally aged and photoaged skin. Therefore, anti-wrinkle or anti-wrinkle means delaying, suppressing, or improving the formation of wrinkles.

In one embodiment of the present invention, “prevention” means suppressing skin antioxidants, suppressing inflammation, suppressing pigmentation, suppressing photoaging, or preventing skin from drying out using a composition containing the plant extract according to the present invention. It refers to any action that suppresses roughness, suppresses the formation of wrinkles, or suppresses or delays other symptoms resulting therefrom. The composition of the present application, which has antioxidant, anti-inflammatory, whitening improvement, photoaging inhibition, moisturizing improvement, and/or anti-wrinkle effects, is used before the initial symptoms or symptoms of skin oxidation, inflammation, pigmentation, photoaging, roughening, or wrinkles appear. It will be apparent to those skilled in the art that these diseases can be prevented by using the composition according to.

In one embodiment of the present invention, “treatment” or “improvement” means suppressing skin antioxidants, suppressing inflammation, suppressing pigmentation, or suppressing photoaging using a composition containing the plant extract according to the present invention. It refers to any action that suppresses dry and rough skin, suppresses the formation of wrinkles, or improves or beneficially changes other symptoms caused by this. In particular, when the composition of the present invention is used for the purpose of treating a disease, the target disease may be freckles, freckles, or age spots caused by inflammation or pigmentation. Additionally, when the composition of the present invention is used for the purpose of treating a disease, the form may be an external preparation, but is not limited thereto.

As used herein, “cosmetic composition” refers to a composition for improving or improving skin oxidation, inflammation, pigmentation, photoaging, roughening, or wrinkles, and other symptoms resulting therefrom. Cosmetic compositions containing the composition of the present invention as an active ingredient include lotion, nutritional lotion, nutritional essence, massage cream, beauty bath water additive, body lotion, body milk, bath oil, baby oil, baby powder, shower gel, shower cream, and sunscreen. Screen lotion, sunscreen cream, suntan cream, skin lotion, skin cream, sunscreen cosmetics, cleansing milk, depilator {cosmetic}, face and body lotion, face and body cream, skin whitening cream, hand lotion, hair lotion, Cosmetic cream, jasmine oil, bath soap, liquid soap, beauty soap, shampoo, hand sanitizer (hand cleaner), medicated soap (non-medical use), cream soap, facial wash, hair rinse, toilet soap, teeth whitening gel, toothpaste, etc. It can be manufactured in the form of. To this end, the composition of the present invention may further include an appropriate carrier, excipient, or diluent commonly used in the production of cosmetic compositions. Carriers, excipients, or diluents that can be further added to the cosmetic composition of the present invention include purified water, oil, wax, fatty acids, fatty acid alcohols, fatty acid esters, surfactants, humectants, thickeners, antioxidants, viscosity stabilizers, and chelates. These include, but are not limited to, rating agents, buffering agents, lower alcohols, etc. Additionally, whitening agents, moisturizers, vitamins, sunscreens, perfumes, dyes, antibiotics, antibacterial agents, and antifungal agents may be included as needed. Hydrogenated vegetable oil, castor oil, cottonseed oil, olive oil, palm oil, jojoba oil, and avocado oil can be used as the oil, and waxes include beeswax, spermaceti, carnauba, candelilla, montan, ceresin, liquid paraffin, and lanolin. It can be used. Stearic acid, linoleic acid, linolenic acid, and oleic acid can be used as fatty acids, and cetyl alcohol, octyldodecanol, oleyl alcohol, panthenol, lanolin alcohol, stearyl alcohol, and hexadecanol can be used as fatty alcohols. Isopropyl myristate, isopropyl palmitate, and butyl stearate can be used as fatty acid esters. As surfactants, cationic surfactants, anionic surfactants, and nonionic surfactants known in the art can be used, and surfactants derived from natural products are preferred whenever possible. In addition, it may contain moisture absorbents, thickeners, antioxidants, etc., which are widely known in the cosmetics field, and their types and amounts are known in the art.

As used herein, “food composition” refers to a composition for improving or improving skin oxidation, inflammation, pigmentation, photoaging, roughening, or wrinkles, and other symptoms resulting therefrom. Food compositions containing the composition of the present invention include various types of foods, such as beverages, milk, dairy products containing lactic acid bacteria, gum, tea, vitamin complexes, powders, granules, pills, tablets, blocks, capsules, confectionery, rice cakes, bread, It can be manufactured in the form of a gel or the like. The food composition of the present invention is composed of natural foods and fermented products thereof with little toxicity and side effects, so it can be used safely even when taken for a long period of time. When the food composition of the present invention is included in food, it can be added in an amount of 0.1 to 100% of the total weight. Here, when the food composition is manufactured in the form of a beverage, there are no particular limitations other than containing the food composition in the indicated ratio, and various flavoring agents or natural carbohydrates can be contained as additional ingredients like ordinary beverages. That is, natural carbohydrates may include monosaccharides such as glucose, disaccharides such as fructose, polysaccharides such as starch, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. there is. Examples of the flavoring agent include natural flavoring agents (thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.). In addition, the food composition of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, and protective colloidal thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. These ingredients can be used independently or in combination. The proportion of these additives is not that critical, but is generally selected in the range of 0.1 to 100 parts by weight per 100 parts by weight of the composition of the present invention.

As used herein, “pharmaceutical composition” means a composition administered for a specific purpose. For the purpose of the present invention, the pharmaceutical composition of the present invention contains plant extracts, specifically complex extracts of pansy, gooseberry, and dogwood as active ingredients and is used to prevent skin oxidation, inflammation, pigmentation, photoaging, roughness, or wrinkles. and administered to improve or treat other symptoms resulting therefrom. In the present invention, the specific target disease may be freckles, freckles, or age spots due to inflammation or pigmentation, and compounds and pharmaceuticals involved therein may be used. May contain acceptable carriers, excipients or diluents. In addition, the pharmaceutical composition according to the present invention contains 0.01 to 50% by weight of the active ingredient in the entire pharmaceutical composition of the present invention based on the total weight of the composition. Carriers, excipients and diluents that may be included in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, Examples include, but are not limited to, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

In one embodiment of the present invention, a cosmetic composition is provided including any one or more selected from the group consisting of Panchaemia chinensis, gooseberry, and dogwood, wherein the cosmetic composition includes Panchamois chinensis, gooseberry, and dogwood. It provides a composition, wherein the cosmetic composition includes at least one selected from the group consisting of stems, leaves, flowers, bark, fruits, and roots of pansy, gooseberry, or dogwood, The cosmetic composition provides a cosmetic composition comprising at least one selected from the group consisting of Pan-fancier extract, gooseberry extract, and dogwood extract, wherein the cosmetic composition comprises Pan-fancier extract, gooseberry extract, and dogwood extract. It provides a cosmetic composition, wherein the extract is obtained by extracting pansy, gooseberry, or dogwood using purified water, butylene glycol, or a mixed solvent thereof, and the cosmetic composition provides skin antioxidant, Provided is a cosmetic composition that has anti-inflammatory, whitening, anti-aging, anti-aging, moisturizing, or anti-wrinkle functions. At this time, the cosmetic composition includes lotion, nutritional lotion, nutritional essence, massage cream, beauty bath water additive, body lotion, body milk, bath oil, baby oil, baby powder, shower gel, shower cream, sunscreen lotion, and sunscreen cream. , suntan cream, skin lotion, skin cream, sunscreen cosmetics, cleansing milk, depilator {cosmetic}, face and body lotion, face and body cream, skin whitening cream, hand lotion, hair lotion, cosmetic cream, jasmine oil , bath soap, liquid soap, beauty soap, shampoo, hand sanitizer (hand cleaner), medicated soap (non-medical use), cream soap, facial wash, hair rinse, cosmetic soap, teeth whitening gel, or toothpaste. You can.

In another embodiment of the present invention, a food composition is provided comprising at least one selected from the group consisting of Pandandelion chinensis, gooseberry, and dogwood, wherein the food composition includes Panchaunnia chinensis, gooseberry, and dogwood. Provided is a food composition comprising at least one selected from the group consisting of stems, leaves, flowers, bark, fruits, and roots of pansy, gooseberry, or dogwood. The food composition provides a food composition comprising any one or more selected from the group consisting of Pan-fancier extract, gooseberry extract, and dogwood extract, wherein the food composition comprises Pan-fancier extract, gooseberry extract, and dogwood extract. Provided is a food composition, wherein the extract is obtained by extracting pansy, gooseberry, or dogwood using purified water, butylene glycol, or a mixed solvent thereof, and the food composition provides skin antioxidant, Provided is a food composition that has anti-inflammatory, whitening, anti-aging, anti-aging, moisturizing, or anti-wrinkle functions. At this time, the food composition may be manufactured in the form of beverages, milk, dairy products containing lactic acid bacteria, gum, tea, vitamin complexes, powders, granules, pills, tablets, blocks, capsules, snacks, rice cakes, bread, gels, etc. .

In another embodiment of the present invention, a pharmaceutical composition for preventing or treating inflammation, freckles, freckles, or age spots is provided, comprising at least one selected from the group consisting of pansy, gooseberry, and dogwood, and the pharmaceutical composition Provides a pharmaceutical composition comprising Pandanum chinensis, gooseberry, and dogwood, wherein the pharmaceutical composition is any selected from the group consisting of stems, leaves, flowers, bark, fruits, and roots of Panchaumnia chinensis, gooseberry, or dogwood. It provides a pharmaceutical composition comprising at least one, wherein the pharmaceutical composition includes at least one selected from the group consisting of Panchaemia extract, gooseberry extract, and dogwood extract, and the pharmaceutical composition Provides a pharmaceutical composition comprising a Pandanum fanta extract, a gooseberry extract, and a dogwood extract, wherein the extract is obtained by extracting Panchaporium chinensis, gooseberry, or dogwood using purified water, butylene glycol, or a mixed solvent thereof. Provided is a pharmaceutical composition. At this time, the pharmaceutical composition may be prepared in the form of an external preparation.

In another embodiment of the present invention, preparing an extract by adding purified water, butylene glycol, or a mixed solvent thereof to any one or more selected from the group consisting of pansy, gooseberry, and dogwood. A method for producing a cosmetic composition, a food composition, or a pharmaceutical composition is provided, the method comprising: preparing an extract by adding purified water, butylene glycol, or a mixed solvent thereof to a mixture of pansy, gooseberry, and dogwood; It provides a method for producing a cosmetic composition, food composition, or pharmaceutical composition further comprising. The extract may be prepared by a method selected from the group consisting of solvent extraction, ultrasonic extraction, supercritical extraction, hot water extraction, high-frequency extraction, fermentation, and fermentation, and is preferably prepared by hot-water extraction or high-frequency extraction. It can be, and more preferably, it can be manufactured by high-frequency extraction method. When preparing an extract using the hot water extraction method or the high-frequency extraction method, the solvent may be a solvent selected from the group consisting of water, ethanol, organic solvents, and mixed solvents thereof, and is preferably purified water, butylene glycol, Or it may be a mixed solvent thereof.

Hereinafter, the present invention will be described in detail step by step.

The extract containing pansy, gooseberry, and dogwood in combination of the present invention has excellent skin antioxidant, anti-inflammatory, whitening improvement, photoaging suppression, moisturizing, and/or anti-wrinkle effects, and is therefore expected to be widely used in the beauty field. .

1 to 27 below are according to an embodiment of the present invention,
Figure 1 is a diagram showing the results of an antioxidant activity test using DPPH of a hot water extract of Panchae root.
Figure 2 is a diagram showing the results of an antioxidant activity test using human skin fibroblasts of the hot water extract of Panchaemia root.
Figure 3 is a diagram showing the results of an anti-inflammatory effect test using macrophages of the hot water extract of Panchae root.
Figure 4 is a diagram showing the results of an antioxidant activity test using DPPH of Indian gooseberry fruit hot water extract.
Figure 5 is a diagram showing the results of an antioxidant activity test of Indian gooseberry fruit hot water extract using human skin fibroblasts.
Figure 6 is a diagram showing the results of an anti-inflammatory effect test using macrophages of Indian gooseberry fruit hot water extract.
Figure 7 is a diagram showing the results of a test for the effect of Indian gooseberry fruit hot water extract on inhibiting photoaging caused by UVB.
Figure 8 is a diagram showing the results of a whitening effect test using the tyrosinase inhibitory activity of Indian gooseberry fruit hot water extract.
Figure 9 is a diagram showing the results of an antioxidant activity test using DPPH of dogwood hot water extract.
Figure 10 is a diagram showing the results of an antioxidant activity test of dogwood hot water extract using human skin fibroblasts.
Figure 11 is a diagram showing the results of a whitening effect test using the tyrosinase inhibitory activity of dogwood hot water extract.
Figure 12 is a diagram showing the results of an antioxidant activity test using human skin fibroblasts of the high-frequency extract of Panchae root.
Figure 13 is a diagram showing the results of an antioxidant activity test of dogwood high-frequency extract using human skin fibroblasts.
Figure 14 is a diagram showing the results of an antioxidant activity test using DPPH of hot water extracts of pansy root, Indian gooseberry fruit, and dogwood.
Figure 15 is a diagram showing the results of an antioxidant activity test using human skin fibroblasts of pansy root, Indian gooseberry fruit, and dogwood hot water extracts.
Figure 16 is a diagram showing the results of measuring the increase in expression of antioxidant factors Nrf2 and AP1 in hot water extracts of pansy root, Indian gooseberry fruit, and dogwood.
Figure 17 is a diagram showing the results of a test for the effect of UVB-induced photoaging inhibition of hot water extracts of pansy root, Indian gooseberry fruit, and dogwood.
Figure 18 is a diagram showing the whitening effect test results of pansy root, Indian gooseberry fruit, and dogwood hot water extract.
Figure 19 is a diagram showing the results of measuring the increase in expression of moisturizing factors HAS2 and AQP3 in hot water extracts of pansy root, Indian gooseberry fruit, and dogwood.
Figure 20 is a diagram showing the results of an antioxidant activity test using DPPH of high-frequency extracts of pansy root, Indian gooseberry fruit, and dogwood tree.
Figure 21 is a diagram showing the results of an antioxidant activity test using human skin fibroblasts of high-frequency extracts of pansy root, Indian gooseberry fruit, and dogwood.
Figure 22 is a diagram showing the results of measuring the increase in expression of antioxidant factors Nrf2, AP1, and TRX in high-frequency extracts of pansy root, Indian gooseberry fruit, and dogwood tree.
Figure 23 is a diagram showing the results of a test for the effect of UVA-induced photoaging inhibition of high-frequency extracts of pansy root, Indian gooseberry fruit, and dogwood tree.
Figure 24 is a diagram showing the results of a test for the effect of UVB-induced photoaging inhibition of high-frequency extracts of pansy root, Indian gooseberry fruit, and dogwood tree.
Figure 25 is a diagram showing the results of an anti-inflammatory effect test of pansy root, Indian gooseberry fruit, and dogwood high-frequency extracts.
Figure 26 is a diagram showing the whitening effect test results of pansy root, Indian gooseberry fruit, and dogwood high-frequency extracts.
Figure 27 is a diagram showing the results of measuring the increase in expression of moisturizing factors HAS2 and AQP3 of high-frequency extracts of pansy root, Indian gooseberry fruit, and dogwood.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

[Experimental Method]

1-1. Preparation of single plant hot water extract

To produce a single plant hydrothermal extract, pansy root, Indian gooseberry fruit, or dogwood plant is used, and a mixed solvent of 1-15 parts by weight of purified water and butylene glycol (butylene glycol) is added based on the dried raw material of the plant. Concentration: 10-50% by volume) was added and subjected to hot water extraction at a temperature of 45-80°C for 6-48 hours. Afterwards, the extract was filtered for clarification at 0.45 μm, then filtered for sterilization at 0.2 μm, and an efficacy test was conducted.

1-2. Preparation of single plant high-frequency extract

To produce a single plant high-frequency extract, pandanum root, Indian gooseberry fruit, or dogwood plant is used, and a mixed solvent of purified water and butylene glycol (butylene glycol) of 1-15 parts by weight based on the dried raw material of the plant is used. Concentration: 10-50% by volume) was added and subjected to high-frequency extraction at room temperature. The high-frequency extraction was performed by applying a frequency of 900 to 1000 kHz (970 kHz) or a power of 200 to 400 W for 2-4 hours. Afterwards, the extract was filtered for clarification at 0.45 μm, then filtered for sterilization at 0.2 μm, and an efficacy test was conducted.

1-3. Preparation of complex plant thermal water extract

To produce a complex plant hot water extract, pansy root, Indian gooseberry fruit, and dogwood plants were used, and a mixed solvent of 1-15 parts by weight of purified water and butylene glycol (butylene glycol) was added based on the dried raw materials of the plants. Concentration: 10-50% by volume) was added and subjected to hot water extraction at a temperature of 45-80°C for 6-48 hours. At this time, the plant ratio was prepared by mixing pansy root, Indian gooseberry fruit, and dogwood in a ratio of 0.5:0.5:1. Afterwards, the extract was filtered for clarification at 0.45 μm, then filtered for sterilization at 0.2 μm, and an efficacy test was conducted.

1-4. Preparation of complex plant high-frequency extract

For the production of complex plant high-frequency extracts, pansy root, Indian gooseberry fruit, and dogwood plants were used, and a mixed solvent of 1-15 parts by weight of purified water and butylene glycol (butylene glycol) was added based on the dried raw materials of the plants. Concentration: 10-50% by volume) was added and subjected to high-frequency extraction at room temperature. At this time, the plant ratio was prepared by mixing pansy root, Indian gooseberry fruit, and dogwood in a ratio of 0.5:0.5:1. The high-frequency extraction was performed by applying a frequency of 900 to 1000 kHz (970 kHz) or a power of 200 to 400 W for 2-4 hours. Afterwards, the extract was filtered for clarification at 0.45 μm and then filtered for sterilization at 0.2 μm, and an efficacy test was conducted.

2. Efficacy testing of plant extracts

2-1. Antioxidant efficacy test using DPPH

Antioxidant activity was measured using 1,1-diphenyl-2 picrylhydrazyl radical (DPPH). In a 96-well plate, 0.2 mM DPPH reagent and extract per well were mixed at a ratio of 1:1, reacted at room temperature for 20 minutes, and absorbance was measured at 517 nm using a microplate reader.

2-2. Antioxidant efficacy test using human skin fibroblasts

After artificially treating human dermal fibroblasts neonatal (HDFn) with H ₂ O ₂ , the ROS inhibitory effect was evaluated using fluorescent substances. HDFn cells were cultured in a black 96-well plate at 37°C and 5% CO ₂ for 24 hours. After 24 hours, the medium was replaced with new medium, and extracts were administered at different concentrations and cultured for an additional hour. Afterwards, the cells were washed with saline solution, treated with H ₂ O ₂ and DCFH-DA, reacted at 37°C for 30 minutes, and then the absorption wavelength was measured at 485 nm and the emission wavelength was measured at 530 nm.

2-3. Antioxidant efficacy test using real-time PCR

Changes in antioxidant gene expression were confirmed by treating HDFn (Human Dermal Fibroblast neonatal) cells with the extract. HDFn cells were cultured in a 96-well plate at 37°C and 5% CO ₂ for 24 hours. After 24 hours, the medium was replaced with new medium, and extracts were administered at different concentrations and cultured for an additional 24 hours. After incubation, it was thoroughly washed with saline solution, RNA was extracted, cDNA was synthesized, and 100 ng/mL was quantified to contain the antioxidant factors Nrf2 (Nuclear factor erythroid-2-related factor 2), AP1 (Activator protein 1), or TRX (Thioredoxin). Real-time PCR was performed. The relative expression rate was normalized to the housekeeping gene (β-actin).

2-4. Anti-inflammatory efficacy test

After artificially treating RAW 264.7 cells (murine macrophage cells) with LPS, an inflammation-causing substance, the inflammation-inhibiting effect was evaluated. RAW 264.7 cells were cultured in a 96-well plate at 37°C and 5% CO ₂ for 24 hours. After 24 hours, the medium was replaced with a new one, and LPS (1 ug/mL) and extract were administered at different concentrations and cultured for an additional 24 hours. After incubation, the medium supernatant was reacted 1:1 with Griess reagent and the absorbance was measured at 570 nm.

2-5. Photoaging inhibition efficacy test

After artificially treating HDFn (Human Dermal Fibroblast neonatal) cells with UVB, the ROS inhibition effect was evaluated using fluorescent substances. HDFn cells were cultured in a 48-well plate at 37°C and 5% CO ₂ for 24 hours. After 24 hours, the medium was replaced with new medium, and extracts were administered at different concentrations and cultured for an additional hour. After incubation, the cells were washed with saline solution, treated with DCFH-DA, and cultured at 37°C for 1 hour. After washing with saline for 1 hour, saline was dispensed into the well, irradiated with UVA or UVB, and the absorption wavelength at 485 nm and the emission wavelength at 530 nm were measured.

2-6. Whitening efficacy test

The whitening effect was measured using tyrosinase inhibition activity. In a 96-well plate, 80 μL of 0.1 M sodium phosphate buffer (pH 6.8) per well, 40 μL of extract, and 40 μL of Tyrosianse from Mushroom were mixed and reacted at 37°C for 3 minutes. After reaction, 40 μL of 5 mM L-DOPA was added and the absorbance was measured at 475 nm using a microplate reader.

2-7. Skin moisturizing efficacy test

Changes in moisturizing-related gene expression were confirmed by treating HDFn (Human Dermal Fibroblast neonatal) cells with the extract. HDFn cells were cultured in a 96-well plate at 37°C and 5% CO ₂ for 24 hours. After 24 hours, the medium was replaced with new medium, and extracts were administered at different concentrations and cultured for an additional 24 hours. After incubation, the samples were thoroughly washed with saline solution, RNA was extracted, cDNA was synthesized, and 100 ng/mL were quantified, and the moisturizing factors HAS2 (Hyaluronan Synthase 2) or QP3 (Aquaporin-3) were subjected to real-time PCR. The relative expression rate was normalized to the housekeeping gene (β-actin).

[Experiment result]

1. Skin improvement effect of single plant thermal water extract

As a single plant, the effects of hot water extracts of pansy root, Indian gooseberry fruit, or dogwood plant on skin antioxidant, anti-inflammatory, whitening, photoaging, or moisturizing were tested.

1-1. Skin-improving effect of Panchae root thermal water extract

As a result of the test, in the antioxidant efficacy test using DPPH, the hot water extract of Pansyanax root showed DPPH scavenging activity as the concentration increased, and a SC ₅₀ (50% Scavenging concentration) value of 14.41% (v/v) was confirmed (Figure 1 ). In addition, as a result of the antioxidant efficacy test using HDFn, there was a 34.9% improved antioxidant efficacy compared to the negative control group (extract untreated) at an extract concentration of 2% (v/v) (Figure 2), and a 56.0% improved anti-inflammatory efficacy (Figure 3).

1-2. Skin improvement effect of Indian gooseberry fruit thermal water extract

As a result of the test, in the antioxidant efficacy test using DPPH, the Indian gooseberry fruit hot water extract showed DPPH scavenging activity as the concentration increased, and a SC ₅₀ (50% Scavenging concentration) value of 0.06% (v/v) was confirmed (Figure 4). In addition, as a result of the antioxidant efficacy test using HDFn, there was an 80.4% improved antioxidant efficacy compared to the negative control group (extract untreated) at an extract concentration of 2% (v/v) (Figure 5), and a 56.0% improved anti-inflammatory efficacy (Figure 6). There was a 20.6% improved efficacy of suppressing photoaging caused by UVB (Figure 7). In the whitening efficacy test results, the extract showed L-DOPA inhibitory activity in a concentration-dependent manner, and an IC ₅₀ (50% inhibition concentration) value of 0.34% was confirmed (FIG. 8).

1-3. Skin improvement effect of dogwood thermal water extract

As a result of the test, in the antioxidant efficacy test using DPPH, the dogwood hot water extract showed DPPH scavenging activity as the concentration increased, and a SC ₅₀ (50% Scavenging concentration) value of 0.83% (v/v) was confirmed (Figure 9 ). In addition, as a result of the antioxidant efficacy test using HDFn, the antioxidant efficacy was improved by 35.1% compared to the negative control group (extract untreated) at the extract concentration of 0.5% (v/v) (FIG. 10). In the whitening efficacy test results, the extract showed L-DOPA inhibitory activity in a concentration-dependent manner, and an IC ₅₀ (50% inhibition concentration) value of 1.67% was confirmed (FIG. 11).

2. Skin improvement effect of single plant high-frequency extract

As a single plant, the effects of hot water extracts of pansy root, Indian gooseberry fruit, or dogwood plant on skin antioxidant, anti-inflammatory, whitening, photoaging, or moisturizing were tested.

2-1. Skin-improving effect of Panchae root high-frequency extract

As a result of the antioxidant efficacy test using HDFn, the antioxidant efficacy was improved by 16.2% compared to the negative control group (extract untreated) at an extract concentration of 2% (v/v) (FIG. 12).

2-2. Skin improvement effect of dogwood high-frequency extract

As a result of the antioxidant efficacy test using HDFn, the antioxidant efficacy was improved by 26.1% compared to the negative control group (extract untreated) at an extract concentration of 0.5% (v/v) (FIG. 13).

3. Skin improvement effect of complex plant thermal water extract

As a complex plant, the effects of thermal water extracts of pansy root, Indian gooseberry fruit, and dogwood plant on skin antioxidant, anti-inflammatory, whitening, photoaging, or moisturizing were tested.

As a result of the test, in the antioxidant efficacy test using DPPH, the complex plant hot water extract showed DPPH scavenging activity as the concentration increased, and a SC ₅₀ (50% Scavenging concentration) value of 1.63% (v/v) was confirmed (Figure 14 ). In addition, as a result of the antioxidant efficacy test using HDFn, there was a 51.4% improved antioxidant efficacy compared to the negative control group (extract untreated) at an extract concentration of 2% (v/v) (Figure 15), and the antioxidant factor at an extract concentration of 0.5% (v/v). The expression of Nrf2 and AP1 was increased by 33.8% and 114.4%, respectively, compared to the negative control group (Figure 16). In the UVB-induced photoaging inhibition efficacy test, there was a 22.7% improved photoaging inhibition efficacy compared to the negative control group at an extract concentration of 2% (v/v) (FIG. 17). In the whitening efficacy test results, the extract showed L-DOPA inhibitory activity in a concentration-dependent manner, and an IC ₅₀ (50% inhibition concentration) value of 5.14% was confirmed (FIG. 18). At an extract concentration of 0.5% (v/v), the expression of moisturizing factors HAS2 and AQP3 was found to be increased by 81.5% and 50.5%, respectively, compared to the negative control group (FIG. 19).

4. Skin improvement effect of complex plant high-frequency extract

As a complex plant, the effects of high-frequency extracts of pansy root, Indian gooseberry fruit, and dogwood plant on skin antioxidant, anti-inflammatory, whitening, photoaging, or moisturizing were tested.

As a result of the test, in the antioxidant efficacy test using DPPH, the complex plant hot water extract showed DPPH scavenging activity as the concentration increased, and a SC ₅₀ (50% Scavenging concentration) value of 0.18% (v/v) was confirmed (Figure 20 ). In addition, as a result of the antioxidant efficacy test using HDFn, there was a 69.5% improved antioxidant efficacy compared to the negative control group (extract untreated) at an extract concentration of 2% (v/v) (Figure 21), and the antioxidant factor at an extract concentration of 0.5% (v/v). The expression of Nrf2, AP1, and TRX was increased by 111.4%, 141.2%, and 90.5%, respectively, compared to the negative control group (Figure 22). In the UVA photoaging inhibition efficacy test, there was a 15.4% improved photoaging inhibition efficacy compared to the negative control at an extract concentration of 2% (v/v) (Figure 23), and in the UVB photoaging inhibition efficacy test, there was a 25.7% improved efficacy (Figure 23). 24), there was a 48.7% improved anti-inflammatory effect (Figure 25). In the whitening efficacy test results, the extract showed L-DOPA inhibitory activity in a concentration-dependent manner, and an IC ₅₀ (50% inhibition concentration) value of 0.89% was confirmed (FIG. 26). At an extract concentration of 0.5% (v/v), the expression of moisturizing factors HAS2 and AQP3 was found to be increased by 184.8% and 44.4%, respectively, compared to the negative control group (Figure 27).

5. Summary of skin improvement effects of each extract

Summarizing the above experimental results 1 to 4 on the skin improvement effects of hot water or high-frequency extracts of Panfanasi root, Indian gooseberry fruit, or/and dogwood plant, the single hot water extract of Panfanart root had antioxidant and anti-inflammatory effects, but whitening and photoaging effects. (UVA and UVB) had no effect. The single hot water extract of Indian gooseberry was effective in antioxidant, anti-inflammatory, whitening, and UVB-induced photoaging, but had no inhibitory effect on UVA-induced photoaging. Dogwood single hydrothermal extract was effective in antioxidant and whitening, but was not effective in anti-inflammatory and photoaging (UVA and UVB). The moisturizing effect of the single hydrothermal extracts of pansy root, Indian gooseberry, or dogwood was not tested. A combined hot water extraction of pansy root, Indian gooseberry, and dogwood was found to be effective in antioxidant, whitening, moisturizing, and UVB-induced photoaging, but was not effective in anti-inflammatory and UVA-induced photoaging. Although the single extract of pansy root and the single extract of Indian gooseberry each have anti-inflammatory effects, when they are used in combination with dogwood extract, the loss of anti-inflammatory efficacy is an effect that is difficult to predict, so using the extract in combination is always an added benefit. This means that it does not cause any effect. On the other hand, high-frequency extraction of a combination of pansy root, Indian gooseberry, and dogwood was found to be effective not only in antioxidants, whitening, and moisturizing, but also in anti-inflammatory and photoaging (UVA and UVB) effects. Even if the ingredients are the same, the effects differ depending on the extraction method. It was found that high-frequency extraction had a superior skin improvement effect compared to hot water extraction. However, considering that hot water extraction has a better antioxidant effect than high frequency extraction when extracting pansy root or dogwood alone, high frequency extraction does not always have a superior effect compared to hot water extraction, and the combination of plant type and extraction method may vary. It is interpreted as having a characteristic. In conclusion, the best skin improvement effect is expected when using high-frequency extraction and combination of pansy root, Indian gooseberry, and dogwood through the present invention.

As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (10)

A cosmetic composition containing complex extracts of pansy, gooseberry, and dogwood as active ingredients,
A cosmetic composition wherein the complex extract is extracted at a high frequency of 900 to 1000 kHz.
According to clause 1,
A cosmetic composition wherein the complex extract is extracted from the stems, leaves, flowers, bark, fruits, or roots of pansy, gooseberry, and dogwood.
delete According to clause 1,
The cosmetic composition is for skin antioxidant, anti-inflammatory, whitening improvement, photo-aging inhibition, or moisturizing improvement.
According to clause 1,
The cosmetic composition includes lotion, nutritional lotion, nutritional essence, massage cream, beauty bath water additive, body lotion, body milk, bath oil, baby oil, baby powder, shower gel, shower cream, sunscreen lotion, sunscreen cream, and suntan cream. , skin lotion, skin cream, sunscreen cosmetics, cleansing milk, depilator {cosmetic}, face and body lotion, face and body cream, skin whitening cream, hand lotion, hair lotion, cosmetic cream, jasmine oil, bath soap. Cosmetics manufactured in the form of liquid soap, beauty soap, shampoo, hand sanitizer (hand cleaner), medicated soap (non-medical use), cream soap, facial wash, hair rinse, cosmetic soap, teeth whitening gel, or toothpaste. Composition.
(a) mixing pansy, gooseberry, and dogwood; and
(b) extracting the mixture at a high frequency of 900 to 1000 kHz.
A food composition containing complex extracts of pandanum, gooseberry, and dogwood as active ingredients,
A food composition wherein the complex extract is extracted at a high frequency of 900 to 1000 kHz.
According to clause 7,
The food composition is for skin antioxidant, anti-inflammatory, whitening improvement, photoaging inhibition, or moisturizing improvement.
A pharmaceutical composition for the treatment of inflammation containing complex extracts of pansy, gooseberry, and dogwood as active ingredients,
A pharmaceutical composition wherein the complex extract is extracted at a high frequency of 900 to 1000 kHz.
A pharmaceutical composition for treating blemishes, freckles, or age spots, comprising complex extracts of pansy, gooseberry, and dogwood as active ingredients,
A pharmaceutical composition wherein the complex extract is extracted at a high frequency of 900 to 1000 kHz.