TW201515666A - Multi-stage releasing composite particles containing amino acid marine active factor peptides and manufacturing method thereof - Google Patents

Abstract

The present invention discloses a multi-stage releasing composite particles containing amino acid marine active factor peptides and manufacturing method thereof. The multi-stage releasing composite particles contain a peptide as the structure of multi-stage releasing composite particles, a plurality of marine active factors ionically chelated with the peptide, and an amino acid for strengthening the composite particle structure.

Description

Multi-stage release composite particle containing amino acid marine active factor peptide and preparation method thereof

The technical field of the invention relates to a multi-stage release composite peptide microparticle containing an amino acid-containing marine active factor which is used for the maintenance composition, and is mainly characterized in that the multi-stage release composite peptide microparticle comprises a space for constructing a multi-stage release composite particle space. a peptide, a plurality of ionic marine active factors for ionic chelation with the peptide, and an amino acid for reinforcing the composite particle structure, wherein the peptide and the ionic marine active factor chelate structure are released in multiple stages a composite particle space having a hydrophilicity for filling the amino acid and enhancing the structure of the multi-stage release composite particle space by the hydrogen bond-enhanced peptide and the ionic marine active factor chelation structure provided by the amino acid . The design of the invention consists in constructing a particle space through a molecular design structure, and using the peptide bond, the hydrogen bond and the ionic bond to form a hydrophilic space of the particle space, and can accommodate the hydrophilic component. The ionic marine active factor used in the design of the present invention refers to a multivalent trace element metal ion taken from deep seawater and acting as a cofactor in the biochemical reaction, and the trace elements contained in the deep seawater are rich in diversity and equilibrium characteristics. The skin's calming, antibacterial, anti-inflammatory, detoxification, beauty, anti-aging, improve immunity and metabolism and repair of human cell tissue have important functions and effects. The key ionic marine active factor designed by the invention, in particular, utilizes the deep water of the Great Barrier Reef in Australia or the deep water body of the ocean on the east coast of Taiwan. The multi-stage release composite microparticles containing the amino acid marine active factor peptide of the present invention may further be combined with one selected from the group consisting of a gel, a particle suspension, a homogeneous liquid and an emulsion. The dispersion medium comprising the combination of groups forms a maintenance composition for medical beauty and maintenance.

Most of the general care products are used to maintain the skin and give the skin its elasticity and vitality due to oxidation and aging caused by the external environment. As the age increases, the elasticity and activity of the skin gradually decline. It is not as delicate and delicate as a newborn, so women who love beauty and even men will buy skin care products to wipe the skin to provide the nutrients needed by the skin, reduce its aging and resist oxidation. However, general skin care products are generally wiped on the surface of the skin, but due to the hydrophobic nature of the skin surface, many hydrophilic active nutrients are difficult to enter the skin tissue, so that the pores of the skin can fully absorb the maintenance essence contained in the skin care products. . Therefore, in order to solve the problem that the conventional skin care formula can not effectively percutaneous absorption, the prior art further proposes to use phospholipid to coat the active ingredient to achieve percutaneous absorption, and the phospholipid is immunologically, sensitized and chemically synthesized according to the source. The problem of purification, and the storage conditions are more stringent, and has the threshold of application popularity. In view of the problems of the prior art, in order to solve the problem, the present invention proposes a multi-stage release composite particle containing an amino acid marine active factor peptide, which is composed of natural and essential elements and molecules and has an easy percutaneous absorption. The composite fine particles of the hydrophilic active ingredient are coated to serve as an implementation and basis for improving the above disadvantages. The multi-stage release composite microparticle containing an amino acid-containing marine active factor peptide according to the object of the present invention is mainly characterized in that the multi-stage release composite peptide microparticle comprises a peptide for constructing a multi-stage release composite particle space, and a plurality of An ionic marine active factor ionically chelated with the peptide and an amino acid used to strengthen the composite particle structure.

The invention relates to a multi-stage release composite particle of the amino acid-containing marine active factor peptide of the present invention which can be applied to a maintenance composition, wherein the multi-stage release composite particle comprises a peptide for constructing a multi-stage release composite particle space, a plurality An ionic marine active factor for ionic chelation with the peptide and an amino acid for reinforcing the composite microparticle structure, wherein the peptide comprises a plurality of peptide ionic groups, a plurality of peptide hydrophilic groups And a plurality of peptide hydrophobic groups, wherein the peptide ion group and the ionic ocean active factor are used to chelate a particle space, wherein the particle space is a hydrophilic space for filling the amino acid and The hydrogen bond provided by the amino acid interacts with the hydrophilic group of the peptide to enhance the structure of the particle space of the chelation structure of the peptide and the ionic marine active factor, and the hydrophobic group of the peptide will form on the periphery of the multi-stage release composite particle. A hydrophobic property area.

The invention further discloses a microneedle-type multi-segment release composite particle containing an amino acid marine active factor peptide, wherein the multi-stage release composite particle comprises a peptide for constructing a multi-stage release composite particle space, and the plurality of particles are used for the victory a peptide ion-chelating ionic marine active factor, an amino acid for reinforcing the composite particle structure, and a plurality of microneedles distributed around the periphery of the multi-stage release composite particle, wherein the peptide comprises a plurality of peptide ionic groups a plurality of peptide hydrophilic groups and a plurality of peptide hydrophobic groups, wherein the peptide ion group and the ionic ocean active factor are used to chelate a particle space, wherein the particle space is a hydrophilic space for filling The amino acid, through the hydrogen bond provided by the amino acid and the hydrophilic group of the peptide to enhance the structure of the particle space of the chelation structure of the peptide and the ionic marine active factor, the peptide hydrophobic group Release of composite particles in the multi-stage The periphery forms a hydrophobic characteristic region, and the microneedles are uniformly distributed in the hydrophobic characteristic region.

In particular, there is a composite particle having an ionic composite structure by using an ionic marine active factor and a collagen peptide through molecular design. Surrounded by the sea, Taiwan is rich in sea water resources. In particular, Taiwan's east coast topography is the most special. Due to the steep and steep geographical environment caused by plate extrusion, it is recognized by oceanographers as having the potential to develop "deep seawater" resources. It is internationally recognized that seawater below the ramp (depth > 200 m) is called "deep seawater". This depth is not directly exposed to sunlight throughout the year, and the number of marine organisms is reduced, making the nutrients available to these organisms more available. Reservation. After analysis, it has the advantages of low temperature, rich minerals (microelements) and nutrient salts, clear and clean, few pathogenic bacteria, and its characteristics are different from ordinary surface seawater. It is widely used and is an emerging water resource with multiple utilization value. Although deep seawater contains a variety of trace elements (iron, zinc, molybdenum, manganese, lithium, strontium, copper, strontium) that the human body needs to ingest. Various trace elements play an important role in the human body. For example, iron (Fe) deficiency affects oxygen storage and transport; zinc (Zn) is involved in immune regulation in the body; zinc (Zn) and copper (Cu) and carbohydrates, proteins and lipids in the body Metabolism is related; copper (Cu) and selenium (Se) are related to antioxidant capacity in the body. The multi-stage release composite particle containing the amino acid marine active factor peptide of the invention, the main key component of the ionic marine active factor is refined from the deep ocean deep mineral water body and removes excess sodium ion, especially using the Great Barrier Reef of Australia Or the deep mineral water body on the east coast of Taiwan is a sea-based mineral water body. The advantage is that the ocean current can assist the minerals and trace elements collected by water bodies from different regions to be a more balanced mixture. This will be different from the problem that the general terrestrial mineral water body will produce a variety of minerals and trace elements due to the geographical imbalance and the mineral composition of the self-added mineral solution. Unbalanced mineral uptake will trigger metabolic abnormalities in other minerals. For example, replenishing excess magnesium ions can cause diarrhea or lead to loss of phosphate in the body and supplement with excessive magnesium. Ions will also affect the absorption of iron and zinc ions in the body; supplementation of excess zinc ions will reduce the absorption rate of copper ions; excessive intake of chromium ions will reduce the negative effects of the combination of iron ions and transferrin. In summary, the present invention selects a deep seawater mineral water body to achieve the purpose of the invention, in particular, the deep water of the Great Barrier Reef in Australia or the deep water body fluid of the east coast of Taiwan has the advantages of natural, clean and diverse minerals and trace elements. The invention utilizes deep seawater mineral water as a key component, and provides a balanced and uniform marine active cofactor of tissue and cells, and further combines multi-segment controlled release technology to provide more efficient and continuous repair and maintenance of tissues and cells. .

The multi-stage release composite microparticle containing the amino acid marine active factor peptide of the present invention is mainly characterized in that the multi-stage release composite peptide microparticle comprises a peptide for constructing a multi-stage release composite particle space, and a plurality of peptides for use with the peptide Ionic chelated ionic marine active factor and an amino acid used to strengthen the composite particulate structure. The peptide type described in the present invention is selected from the group consisting of fish collagen peptide, algal collagen peptide, porcine collagen peptide, bovine collagen peptide, collagen peptide, plant collagen peptide and the collagen peptide decomposition peptide A combination of the constituent groups, and the amino acid is also formed from the above-mentioned collagen peptide molecule. Collagen peptides in normal human skin are gradually lost due to age and various reasons, and the speed at which the body produces collagen peptides and collagen is far behind the rate of consumption. The key to the design of the present invention is to design a multi-stage release composite particle containing an amino acid marine active factor peptide, and provide a plurality of amino acids, peptides and trace elements required for human tissues. Therefore, the present invention also incorporates another key component, the deep ocean mineral water, through molecular design, in particular, the use of the deep water of the Great Barrier Reef in Australia or the deep water of the ocean on the east coast of Taiwan. These water bodies contain rich and balanced trace elements and minerals which have important effects and functions on skin stabilization, bacteriostatic, anti-inflammatory, detoxification, beauty, anti-aging, immune enhancement and metabolism of human cells. The invention includes The multi-stage release composite microparticle of the amino acid marine active factor peptide, preferably further comprising a plurality of vitamin components or cysteine derivatives selected from the group consisting of vitamin C, vitamin B1, vitamin B2, vitamin B3, Vitamin B5, vitamin B6, vitamin B7, vitamin B9, vitamin B12 and combinations thereof. The cysteine derivative is then selected from the group consisting of various alkyl substituted cysteamines, various alkyl substituted cysteamines, and combinations thereof. The cysteine derivative can block or interfere with the mechanism of melanogenesis in the basal layer where the epidermis and dermis intersect. When the melanocytes in the skin are exposed to ultraviolet light, the production of tyrosinase is accelerated, and tyrosinase stimulates the tyrosine oxidation of the basal layer to melanin. The cysteine derivative competes with tyrosine and is more susceptible to oxidation than tyrosine, thereby preventing the formation of black pigment by a competitive mechanism. Vitamin C, which is a multivitamin ingredient, is an excellent antioxidant that prevents tyrosine from being oxidized to melanin. It is an important component that inhibits melanin production and also helps cysteine derivatives inhibit melanin production. The multi-stage release composite microparticle containing the amino acid marine active factor peptide of the present invention can be used as a key functionality of a gel type, an oil-in-water type emulsified type or a water-in-oil type emulsified skin care composition, a cosmetic or an external preparation. Ingredients. That is, the present invention further discloses a multi-stage release composite microparticle maintenance composition containing an amino acid marine active factor peptide, wherein the multi-stage release composite microparticle maintenance composition comprises a container having an accommodation space; a multi-stage release peptide of the composite particle space; a plurality of ionic marine active factors for ionic chelation with the peptide; an amino acid for reinforcing the composite particle structure; and a dispersion medium, wherein the peptide Containing a plurality of peptide ionic groups, a plurality of peptide hydrophilic groups, and a plurality of wins a peptide hydrophobic group, wherein the peptide ionic group and the ionic marine active factor are used to sequester a particle space, wherein the particle space is a hydrophilic space for filling the amino acid and passing through the amino acid The hydrogen bond is provided to interact with the hydrophilic group of the peptide to enhance the structure of the particle space of the chelation structure of the peptide and the ionic marine active factor, and the hydrophobic group of the peptide forms a hydrophobic characteristic region around the multi-stage release composite particle. The accommodating space is for inserting the multi-stage release composite particles and the dispersion medium, the multi-stage release composite particles and the dispersion medium are uniformly mixed before being placed in the accommodating space; and the dispersion medium system is selected from the group consisting of Glue, particle suspension, homogeneous liquid, emulsion, capsule filling liquid, microcrystalline forming liquid, microcapsule forming liquid, tablet excipient, medical beauty treatment, calming repairing liquid, moisturizing whitening filling agent, facial mask filling liquid, function A group of dressing fillers, pastes, oils, volatile solvents, gaseous carriers, combinations thereof, and the like.

The present invention extends to disclose a microneedle-type multi-stage release composite microparticle maintenance composition containing an amino acid marine active factor peptide, wherein the microneedle multi-stage release composite microparticle maintenance composition comprises a container having an accommodation space a peptide for constructing a multi-stage release composite particle space; a plurality of ionic marine active factors for ionic chelation with the peptide; an amino acid for strengthening the composite particle structure; a plurality of microneedles; And a dispersing medium, wherein the peptide comprises a plurality of peptide ionic groups, a plurality of peptide hydrophilic groups, and a plurality of peptide hydrophobic groups, wherein the peptide ionic group and the ionic marine active factor are utilized Chelating framework is a particle space, which is a hydrophilic space for filling the amino acid, and strengthens the peptide and ionic marine activity through the hydrogen bond provided by the amino acid and the hydrophilic group of the peptide. The structure of the particle space of the chelation structure of the factor, the hydrophobic group of the peptide will release the composite particle in the multi-stage Forming a hydrophobic characteristic region on the periphery; the accommodating space is for inserting the multi-stage release composite particles and the dispersion medium, and the multi-stage release composite particles and the dispersion medium are uniformly mixed before being placed in the accommodating space; The needle system is refined by using a tissue having a fresh water sponge body of a bone needle and a sponge thread; and the dispersion medium system is selected from the group consisting of a gel, a particle suspension, a uniform liquid, an emulsion, a capsule filling liquid, a microcrystalline forming liquid, and a microcapsule. Forming liquid, tablet excipient, medical beauty treatment, calming and repairing liquid, moisturizing whitening filling agent, facial filling liquid, functional dressing filling, cream, oil, volatile solvent, gas carrier and combination thereof The group of people.

The multi-stage release composite microparticles containing the amino acid marine active factor peptide of the present invention can also be mixed with water, ethanol and any drinkable liquid to prepare a health food or drink. When the present invention proposes that the multi-stage release composite microparticle containing the amino acid marine active factor peptide is applied to a food or drink form, the multi-stage release composite particle accounts for 0.1 to 1.0 weight percent of the beverage formulation. The ionic active factor chelated in the multi-stage release composite microparticles comprises a plurality of minerals selected from the group consisting of potassium, calcium, iron, zinc, molybdenum, manganese, lithium, cerium, copper, cerium or combinations thereof. Further, the food or drink prepared by the present invention may comprise a food or drink supplement, and the food or drink supplement may comprise a sweetener, a food coloring, a nutritional additive, an amino acid, a preservative, a fragrance, a thickener, A color retention agent, an antioxidant, a flavoring agent, or a combination thereof. In a particular example, the sweetener can comprise high fructose syrup, sucrose, maltitol syrup, oligosaccharides, stevia, lactose, glucose, or a combination thereof. The nutritional supplement may comprise vitamin C, thiamine, riboflavin, niacin, pantothenic acid, biotin, folic acid, cobalamin, pyridoxine hydrochloride or a combination thereof. The food coloring matter may comprise safflower yellow pigment, curcumin, erythropoietin, copper chlorophyll sodium, iron chlorophyll sodium, lutein or a combination thereof. The amino acid may comprise arginine, lysine, glycine, arginine, lysine, leucine, isoleucine, valine, Aspartic acid or a combination thereof.

The invention discloses a multi-stage release composite particle containing an amino acid marine active factor peptide, as shown in FIG. 1 , FIG. 2 and FIG. 3 :

10‧‧‧ cover

20‧‧‧ container body

30‧‧‧ container opening

35‧‧‧Foldable container body opening

40‧‧‧ accommodating space

50‧‧‧ cover thread

55‧‧‧ screw joint

100‧‧‧Collagen peptide-compounding marine active cofactor

200‧‧‧Distributed media

300‧‧‧ functional flower ingredients

1000‧‧‧ peptide hydrophobic group

2000‧‧‧ peptide hydrophilic group

3000‧‧‧ peptide ionic group

4000‧‧‧Ionic marine active factor

5000‧‧‧Amino acid

6000‧‧‧Particle space

7000‧‧‧hydrophobic area

8000‧‧‧microneedle

Fig. 1 is a schematic view showing the three-dimensional structure of a multi-stage release composite particle containing an amino acid marine active factor peptide.

Fig. 2 is a schematic view showing a microneedle type multi-stage release composite particle containing an amino acid marine active factor peptide.

Fig. 3 is a schematic view showing the application of a maintenance product of a multi-stage release composite particle containing an amino acid marine active factor peptide.

First Embodiment: Multi-stage release composite particle containing amino acid marine active factor peptide and preparation thereof

The invention discloses a multi-stage release composite microparticle 100 containing an amino acid marine active factor peptide, as shown in FIG. 1 , which particularly comprises a skin which can be dispersed in the skin through a dispersion medium to provide skin care effect and at the same time achieve calm skin. Effective maintenance of active composite particles. The multi-stage release composite microparticle 100 containing the amino acid marine active factor peptide of the present invention is mainly characterized in that the multi-stage release composite peptide microparticle comprises a peptide for constructing a multi-stage release composite particle space, and a plurality of peptides for use with the peptide a chelated ionic marine active factor and an amino acid for enhancing the composite microparticle structure, wherein the peptide agonist group 3000 and the ionic marine active factor 4000 chelate structure are a multi-stage release composite particle space, The particulate space is hydrophilic to fill the amino acid 5000, and the hydrogen bond provided by the amino acid interacts with the peptide hydrophilic group 2000 to enhance the chelation structure of the peptide and the ionic marine active factor. Release the knot of the composite particle space Structure. The design of the present invention is to release the composite particle space through a plurality of stages through the molecular design framework, and use the peptide bond, the hydrogen bond and the ionic bond to make the multi-stage release composite particle space 6000 form a hydrophilic space, and can accommodate the hydrophilic component. The multi-segment release complex particle of the peptide hydrophobic group 2000 forms a hydrophobic characteristic region 7000. In the preparation of the multi-stage release composite particles of the invention, the particle formation conditions can be known through the particle size analyzer and the dynamic light scattering instrument, and the formation conditions can be confirmed by the fluorescence cursor characterization and fluorescence spectroscopy and viscosity analysis techniques. Further, the multi-stage release composite particles are produced according to the formation conditions obtained by the experiment. The particle size analysis can directly and clearly show the gradual formation of the particles and the change in particle size. Both the particle size analyzer and the dynamic light scattering instrument can be used to analyze the minimum composite particle formation conditions. Under appropriate molding conditions, significant changes in intensity can be observed regardless of the particle size analyzer and the dynamic light scattering instrument. Under such conditions, the fluorescence fluorescence intensity of the system is analyzed by using the fluorescence calibration factor of the fluorescent cursor and the fluorescence spectrum monitoring technology to analyze the ratio of the excited fluorescence intensity of the system to the fluorescence intensity of the monomer (IE/IM). The microenvironmental hydrophobicity characteristic of the composite microparticles is released, and when the composite microparticles are formed, a data break point occurs in the ratio of the excited twin fluorescent radiation intensity to the monomer fluorescence emission intensity (IE/IM). The composite microparticles designed by the invention ionicly chelate most of the cationic ionic marine active factors with the anionic acid groups on the polypeptide, and the positive and negative electric properties are neutralized during the chelating process, and the conductive The degree will also change greatly, and the turning point of the change in conductivity is the optimum condition for forming composite particles. Further, the present invention introduces an amino acid which is decomposed by a multi-peptide by a molecular design concept, and these amino acids are introduced after an ionic chelation reaction, and the amino acids introduced in the second stage can be dispersed due to their hydrophilic properties. The hydrophilic space inside the composite particles has reduced system interfacial activity. The amino acid in the hydrophilic space inside the composite microparticle further provides an amine group and an acid group, such a group will generate a hydrogen bond with the peptide bond of the polypeptide, making the composite particle structure more stable. Use this The composite microparticles prepared in the examples have hydrophobic characteristics on the periphery. When the composite microparticles are added to the system of the maintenance composition and are in contact with the tissue, especially when in contact with the surface layer of the skin, the hydrophobic periphery acts on the surface hydrophobic layer of the skin due to the interfacial energy stability, and the composite microparticles are Disintegrating during the penetration of the surface layer, and discarding the marine active factor and the amino acid, in particular, transferring the marine active factor and the amino acid to the hydrophilic tissue region. Such a disintegration process involves the transfer of the force between the hydrophobic interaction and the chelating ionic bond, the hydrogen bond, and the slow transfer of the balance of the force balance, so that the marine active factor and the amino acid are continuously and multi-stagely compounded. The particles are released. Similarly, many absorption environments in the body must first enter the hydrophilic space through the hydrophobic barrier. These disintegrated ionic marine active factors, amino acids and even peptides are the key components of metabolism and tissue repair in the body. In particular, ionic marine active factors are key contributors to human biochemical reactivity and are even more important. The composite microparticles of the invention comprise abundant ionic marine active factors, and are mainly composed of ionic minerals and trace elements extracted from deep seawater. Because the deep ocean cannot directly direct sunlight (below two hundred meters), the biomass is less than the shallow seawater area. Therefore, the bio-consumption of ionic marine active factors such as minerals and trace elements will be smaller, and the deep seawater will be relatively more Shallow seawater contains rich nutrients, which are richer in minerals and trace elements than terrestrial fresh water, such as rich in sodium (Na), calcium (Ca), potassium (K), iron (Fe), and zinc ( Trace elements of Zn), molybdenum (Mo), manganese (Mn), lithium (Li), strontium (Sr), copper (Cu), cerium (Si) or combinations thereof. The deep seawater may be taken from seawater at a predetermined depth of between 200 and 1,500 meters below sea level, preferably at a predetermined depth of 500 to 700 meters below sea level, more particularly The ionic marine active cofactor is obtained by refining the deep mineral water of the Great Barrier Reef in Australia or the deep mineral water of the East Coast of Taiwan. The use of the peptide species is not particularly limited, and may be appropriate according to its purpose. Selectively, preferably the peptide system uses collagen to win The peptide may be selected from the group consisting of a fish collagen peptide, an algal collagen peptide, a porcine collagen peptide, a bovine collagen peptide, a collagen peptide, a plant collagen peptide, and a group of the collagen peptide peptides. Further, the amino acid may comprise selected from the group consisting of glycine, humative, leucine, isoleucine, alanine, arginine, glutamine, lysine, aspartic acid, bran Proline, valine, cysteine, cyanidin, methionine, histidine, phenylalanine, tyrosine, tryptophan, aspartame, glycine, serine And a combination of amino acids. The addition of amino acid can strengthen the composite microparticle structure, and can provide the structure to gradually release during tissue absorption and provide multi-stage absorption and continuous repair metabolism.

Second Embodiment: Microneedle-type multi-segment release composite microparticle containing amino acid marine active factor peptide and preparation thereof

A second embodiment of the present invention discloses a microneedle-type multi-segment release composite microparticle containing an amino acid marine active factor peptide, as shown in FIG. 2, which particularly comprises a skin maintenance effect which can be dispersed in the skin through a dispersion medium. It can simultaneously achieve micro-needle-type multi-segment release composite particles with minimally invasive skin surface effects. The microneedle-type multi-segment release composite microparticle containing an amino acid marine active factor peptide of the second embodiment of the present invention is mainly characterized in that the multi-stage release composite peptide microparticle comprises a peptide for constructing a multi-stage release composite particle space, and a plurality of uses. An ionic marine active factor ionically chelated with the peptide, an amino acid for enhancing the composite particle structure, and a microneedle dispersed on the outer layer of the multi-stage release complex peptide, wherein the peptide peptide of the peptide The group 3000 and the ionic marine active factor 4000 chelate structure form a multi-stage release composite particle space having a hydrophilicity for filling the amino acid 5000 and transmitting hydrogen bonds and peptides through the amino acid Hydrophilic group 2 000 function to strengthen the structure of the multi-stage release composite particle space 6000 of the chelation structure of the peptide and the ionic marine active factor. The design of the present invention is to release the composite particle space 6000 through a molecular design framework, and use the peptide bond, the peptide hydrophilic group 2000, the hydrogen bond and the ionic bond to make the multi-stage release composite particle space 6000 form a hydrophilic space. It can hold hydrophilic ingredients. The multi-segment release of the peptide hydrophobic peptide 1000 forms a hydrophobic characteristic region 7000. The microneedle 8000 is made of a microneedle sponge tissue having a bone needle and a sponge wire, and achieves the invention to penetrate the skin, accelerate subcutaneous blood circulation and clear lymphatic velocity, and deepen the circulation of the skin to renew collagen regeneration without damaging the skin. The machine is turned down, naturally let the aging keratin remove the design purpose, and strengthen the effect of ionic marine active factor and amino acid deep into the tissue. Preferably, the microneedle system utilizes a freshwater sponge, such as a freshwater sponge in the Danube Valley of Germany.

Third Embodiment: The present invention utilizes a multi-stage release composite particle containing an amino acid marine active factor peptide to maintain a maintenance product.

A third embodiment of the present invention is a maintenance product made by using a multi-stage release composite microparticle containing an amino acid marine active factor peptide, as shown in Fig. 3, comprising a container, an amino acid-containing marine active factor peptide The plurality of segments release the composite particles 100 and a dispersion medium. In the embodiment of the present invention, the container comprises a lid body 10, a container body 20 and a container body opening 30 protruding outwardly at one end of the container body 20, and an accommodating space 40 provided inside the container body; the container body opening 30 The outer portion has a screw portion 55 of an external thread, and the cover body 10 has a cover thread 50 corresponding to the internal thread corresponding to the external thread, so that the cover thread 50 is in close contact with the screw portion 55. The accommodating space 40 can be placed in the multi-stage release composite particle 100 containing the amino acid marine active factor peptide and the dispersion medium 200, Generally, the multi-stage release composite particle 100 containing the amino acid-containing marine active factor peptide and the dispersion medium 200 are uniformly mixed before being placed in the accommodating space 40 to form a plurality of segments of the amino acid-containing marine active factor peptide. A maintenance product that releases the mixture of the composite microparticles 100 and the dispersion medium 200. The multi-stage release composite microparticle 100 containing the amino acid marine active factor peptide and the preparation thereof are as described in Example 1, and are mainly obtained by using collagen peptide and deep ocean water processing of marine ocean, in particular, utilizing the Great Barrier Reef Ocean Deep water or deep water on the east coast of Taiwan. The dispersion medium 200 is selected from the group consisting of a gel, a particle suspension, a uniform liquid, an emulsion, a capsule filling liquid, a microcrystalline forming liquid, a microcapsule forming liquid, a tablet excipient, a medical treatment, a calming and repairing liquid, and a moisturizing whitening. A group consisting of a filler, a mask filling liquid, a functional dressing filling, a paste, a fat, a volatile solvent, a gas carrier, and combinations thereof. The application of the embodiment of the present invention further comprises a plurality of functional fragrant flowers and plants ingredients 300, wherein the functional fragrant flower and grass ingredients 300 are selected from the group consisting of sweet-scented osmanthus, cassia seed, Eucommia ulmoides, lotus leaves, chamomile, bodhi leaves, roses, violets, incense Bee leaf, mate tea, pink rose, verbena, lemon grass, lotus leaf, linden flower, lavender, lemon balm, jasmine leaf, chamomile, jasmine, calendula, rosemary, tomorrow leaf, silk flower, mullein, Spiraea, arborvitae, calendula, sage, sassafras, chuanxiong, lotus leaf, hibiscus, apple blossom, orange blossom, car chrysanthemum, honeysuckle, gold osmanthus, stevia, cornflower. In the skin care application scenario of the embodiment of the present invention, the cover body 10 is separated from the container body 20, and the accommodating space 40 is opened. The user can insert the soft container or pour the rigid container into the accommodating space. A multi-stage release composite particle containing amino acid marine active factor peptide and a dispersion medium mixture, which can be released from the skin with an amino acid-containing marine active factor peptide by spraying or spreading or other means capable of spreading on the skin. The composite particles are in sufficient contact and, through the design of the present invention, pass through the surface layer of the hydrophobic skin, deep into the tissue, and in the process, due to the hydrophobic interaction of the skin surface layer on the multi-stage release of the composite particles, The multi-segment release composite particles gradually disintegrate during the traversing process, and release the marine active factors and amino acids in the structure in multiple stages, so that the deep tissue of the skin fully absorbs the marine active factors and amino acids to achieve the marine active factors. The effect of amino acid on the biochemical metabolism and maintenance of the human body. The cover 10 can also be replaced by a stopper, and in addition to the sealing effect, it can meet the requirements of different use states. The invention discloses a multi-stage release composite particle containing an amino acid marine active factor peptide, in particular, a deep mineral water body of the Great Barrier Reef in Australia or a deep mineral water body of the east coast of Taiwan, and the use of the peptide type is not particularly limited. , preferably a collagen peptide, such as selected from the group consisting of a fish collagen peptide, an algal collagen peptide, a porcine collagen peptide, a bovine collagen peptide, a collagen peptide, a plant collagen peptide, and the collagen peptide peptide A combination of ethnic groups. The multi-stage release composite particles account for between 10% and 60% by weight of the multi-stage release composite particles and the dispersion medium mixture. The dispersion medium may specifically have a modified cellulose polymer having a hydroxyethyl branch in a weight percentage ranging from 0.5 to 1.5 percent; the weight percentage ranges from one percent to one percent Emulsifier to between 5 percent; skin care solvent in the range of 3 to 15 percent by weight; emollient in weight percent ranging from 1 to 10 percent; weight The percentage ranges from 0.5 to 20 percent of the oxoxane compound. The dispersing medium can be further added with functional auxiliary components such as Q10, silk fibroin, hyaluronic acid, levorotin C, pearl powder, vitamins, and cysteine derivatives, wherein Q10 can be Co-Q10, coenzyme Q10, and coenzyme. Enzyme Q10, etc., silk protein may be silk protein peptide amino acid, silk peptide, etc., hyaluronic acid may be N-acetylglucosamine (NAG), glucuronic acid-N-ethylthioglucosamine, hyaluronic acid precursor, etc. L-C can be L-Vitamin C, L-V-C, Vitamin C, C, etc. Pearl powder can be small molecule hydrolyzed pearl powder, vitamins can be vitamin C, vitamin B1, vitamin B2, vitamin B3, Vitamin B5, The vitamin B6, the vitamin B7, the vitamin B9, the vitamin B12, etc., the cysteine derivative may be various alkyl-substituted cysteine acids and various alkyl-substituted cysteamine salts, and the above components are not particularly limited. Appropriate selection can be made depending on its purpose. Specifically, the dispersion medium of the present invention can use eucalyptus oil as a carrier. For example, polyalkyl siloxane, polyalkyl aryl siloxane and polyether decane copolymer, and the like. Preferably, an eucalyptus oil selected from the group consisting of polydimethyl siloxane, poly(methylphenyl) decane, oxyalkylene ether copolymers, and combinations thereof can be used. Alternatively, the esters may also be incorporated into the dispersion vehicle of the present invention as a pharmaceutically acceptable carrier, the esters comprising an alkyl ester of a fatty acid having 10 to 20 carbon atoms, a fatty acid having 10 to 20 carbon atoms. A group of alkenyl esters, ether esters, polyhydric alcohol esters, wax esters, sterol esters, and combinations thereof. For example, methyl, isopropyl, and butyl esters of fatty acids, hexyl laurate, isohexyl laurate, isohexyl palmitate, isopropyl palmitate, decyl oleate, decyl stearic acid Salt, isodecyl oleate, cetyl stearate, isopropyl isostearate, diisopropyl adipate, diisohexyl adipate, dihexyl decyl hexane Acid salts, diisopropyl sebacate, lauryl lactate, tetradecyl lactate and cetyl lactate, oleyl myristate, oleyl stearate and oil base oil Fatty acid esters of acid salts, ethoxylated fatty alcohols, mono- and di-fatty acid esters of ethylene glycol, mono- and di-fatty acid esters of diethylene glycol, polyethylene glycol mono- and di-fatty acids Ester, propylene glycol mono- and di-fatty acid esters, polypropylene glycol monooleate, polypropylene glycol monostearate, ethoxylated propylene glycol monostearate, C Tribasic mono- and di-fatty acid esters, polyglycerol polyfatty acid esters, ethoxylated glycerol monostearate, 1,3-butene glycol monostearate, 1,3 -butene glycol distearate, poly Oxidized ethylene polyol fatty acid ester, dehydrated yam alcohol fatty acid ester, polyoxyethylene sorbitan fatty acid ester, beeswax, whale oil, tetradecyl myristate, stearic acid stearate, cholesterol , fatty acid esters, and the like. Further, the secondary component of the dispersion medium may also contain a preservative, a fragrance, an anti-foaming agent, Sunscreens, colorants, and mixtures thereof, each of which achieves its individual function in its effective dosage. The container may be a rigid bottle container, a soft tubular container, a rigid can container or the like.

The multi-stage release composite microparticle containing the amino acid marine active factor peptide of the invention has the following advantages: (1) The ratio of each ion in the multi-stage release composite microparticle containing the amino acid-containing marine active factor peptide of the invention conforms to human tissues and cells Required. (2) The multi-stage release composite particle of the amino acid-containing marine active factor peptide of the invention has various minerals and trace elements, such as trace elements such as iron, zinc, molybdenum, manganese, lithium, strontium, copper and strontium, and can be The human biochemical reaction metabolic system plays the role of maintaining an active cofactor and is combined with enzymes in the body to promote human metabolism.

The above is only the preferred embodiment of the present invention, and the scope of the present invention is not limited thereto, that is, the equivalent changes and modifications made by the scope of the invention and the contents of the invention are still The scope of the invention is covered. Looking at the above, the structural features of the present invention do have practical value and progress. In terms of its overall structure, Cheng has already complied with the statutory requirements of the patent law, and has filed an invention patent application according to law.

10‧‧‧ cover

20‧‧‧ container body

30‧‧‧ container opening

40‧‧‧ accommodating space

100‧‧‧Multi-stage release complex peptide particles containing amino acid marine active factors

200‧‧‧Distributed media

300‧‧‧Amino acid

1000‧‧‧ peptide hydrophobic group

6000‧‧‧Particle space

7000‧‧‧hydrophobic area

Claims (9)

A multi-stage release composite particle containing an amino acid marine active factor peptide, wherein the multi-stage release composite particle comprises a peptide for constructing a multi-stage release composite particle space; and a plurality of ions for ion chelation with the peptide a marine active factor; and an amino acid for enhancing the composite particle structure, wherein the peptide comprises a plurality of peptide ionic groups, a plurality of peptide hydrophilic groups and a plurality of peptide hydrophobic groups, wherein The peptide ionic group and the ionic marine active factor chelate a particle space, wherein the particle space is a hydrophilic space for filling the amino acid, and the hydrogen bond provided by the amino acid and the peptide are hydrophilic The group acts to strengthen the structure of the particle space of the chelation structure of the peptide and the ionic marine active factor, and the hydrophobic group of the peptide forms a hydrophobic characteristic region around the multi-stage release composite particle, and the multi-stage release composite particle will follow The structure disintegrates and the ionic marine active factor and the amino acid are continuously released in multiple stages. A microneedle-type multi-segment release composite particle containing an amino acid marine active factor peptide, wherein the microneedle type multi-stage release composite particle comprises a peptide for constructing a multi-stage release composite particle space; a plurality of peptides for use with the peptide An ionic chelated ionic marine active factor; an amino acid for reinforcing the composite microparticle structure and a plurality of microneedles distributed around the periphery of the multi-stage release composite microparticle, wherein the peptide comprises a plurality of peptide ionic groups, a plurality of peptide hydrophilic groups and a plurality of peptide hydrophobic groups, wherein the peptide ion group and the ionic ocean active factor are used to chelate a particle space, wherein the particle space is a hydrophilic space for filling the space An amino acid, and through the hydrogen bond provided by the amino acid and the hydrophilic group of the peptide to enhance the peptide and the ionic marine active factor The structure of the particle space of the chelate structure, the hydrophobic group of the peptide forms a hydrophobic characteristic region around the multi-stage release composite particle, and the microneedle is evenly distributed in the hydrophobic characteristic region, and the microneedle-type multi-segment release composite particle will The ionic marine active factor and the amino acid are continuously released in multiple stages as the structure disintegrates. The multi-stage release composite particle according to claim 1, wherein the ionic marine active factor is prepared by using a deep marine mineral water body of the Great Barrier Reef in Australia or a deep mineral water body of the east coast of Taiwan, which is used for the human body. The biochemical reaction is selected from trace elements consisting of sodium, calcium, potassium, iron, zinc, molybdenum, manganese, lithium, cesium, copper, cerium or combinations thereof. The multi-stage release composite microparticle according to claim 1, wherein the peptide is selected from the group consisting of fish collagen peptide, algal collagen peptide, porcine collagen peptide, bovine collagen peptide, collagen peptide, plant collagen. A combination of a peptide and a group of such collagen peptides that decompose the peptide. The multi-stage release composite microparticle according to claim 1, wherein the amino acid comprises arginine, lysine, glycine, arginine, lysine, leucine, isoleucine, Proline, aspartic acid or a combination thereof. The microneedle-type multi-segment release composite microparticle according to claim 2, wherein the amino acid comprises arginine, lysine, glycine, arginine, lysine, leucine, and white An amino acid, proline, aspartic acid or a combination thereof; the peptide is selected from the group consisting of fish collagen peptide, algal collagen peptide, porcine collagen peptide, bovine collagen peptide, collagen peptide, plant collagen a combination of a peptide and a group of such collagen peptides; the ionic marine active factor is a deep ocean mine of the Great Barrier Reef, Australia Prepared by a physical water body or a deep mineral water body of the East Coast of Taiwan, which contains trace elements selected from sodium, calcium, potassium, iron, zinc, molybdenum, manganese, lithium, strontium, copper, strontium or combinations thereof for biochemical reactions in humans. And the microneedle system is refined by using a freshwater sponge body having a bone needle and a sponge wire. A multi-stage release composite microparticle maintenance composition containing an amino acid marine active factor peptide, wherein the multi-stage release composite microparticle maintenance composition comprises a container having an accommodation space; and a structure for constructing a multi-stage release composite particle space a peptide; an ionic marine active factor for ionic chelation with the peptide; an amino acid for enhancing the composite particle structure; and a dispersion medium, wherein the peptide comprises a plurality of peptide ions a group, a plurality of peptide hydrophilic groups and a plurality of peptide hydrophobic groups, wherein the peptide ion group and the ionic ocean active factor are used to chelate a particle space, the particle space being a hydrophilic space Filling the amino acid and strengthening the structure of the particle space of the chelation structure of the peptide and the ionic marine active factor by the hydrogen bond provided by the amino acid and the hydrophilic group of the peptide, the peptide hydrophobic group The cluster forms a hydrophobic characteristic region on the periphery of the multi-stage release composite particle; the accommodating space is used for inserting the multi-stage release composite particle and the dispersion medium, and the multi-segment release The composite microparticles and the dispersing medium are uniformly mixed before being placed in the accommodating space; the dispersing medium system is selected from the group consisting of a gel, a particle suspension, a uniform liquid, an emulsion, a capsule filling liquid, a microcrystalline forming liquid, and a microcapsule forming Liquid, tablet excipient, medical beauty treatment, calming and repairing liquid, moisturizing whitening filling agent, facial filling liquid, functional dressing filling, cream, oil, volatile solvent, gas carrier and combination thereof, etc. Ethnic group; and The multi-stage release of the composite particles releases the ionic marine active factor and the amino acid in multiple stages as the structure disintegrates. . A microneedle type multi-stage release composite microparticle maintenance composition containing an amino acid marine active factor peptide, wherein the microneedle type multi-stage release composite microparticle maintenance composition comprises a container having an accommodation space; a multi-stage release peptide of the composite particle space; a plurality of ionic marine active factors for ionic chelation with the peptide; an amino acid for reinforcing the composite particle structure; a plurality of microneedles; and a dispersion medium Wherein the peptide comprises a plurality of peptide ionic groups, a plurality of peptide hydrophilic groups, and a plurality of peptide hydrophobic groups, wherein the peptide ion group and the ionic ocean active factor are used to chelate a particle Space, the particulate space is a hydrophilic space for filling the amino acid, and the hydrogen bond provided by the amino acid interacts with the hydrophilic group of the peptide to enhance the chelation structure of the peptide and the ionic marine active factor. The structure of the particle space, the hydrophobic group of the peptide forms a hydrophobic characteristic region on the periphery of the multi-stage release composite particle; the accommodating space is used for inserting the multi-stage release composite particle The dispersion medium, the multi-stage release composite particles and the dispersion medium are uniformly mixed before being placed in the accommodating space; the micro-needle system is refined by using a tissue having a fresh water sponge body of a bone needle and a sponge yarn; The medium system is selected from the group consisting of a gel, a particle suspension, a uniform liquid, an emulsion, a capsule filling liquid, a microcrystalline forming liquid, a microcapsule forming liquid, a tablet excipient, a medical beauty treatment calming repairing liquid, a moisturizing whitening filling agent, a group of mask filling fluids, functional dressing fillers, creams, oils, volatile solvents, gas carriers, and combinations thereof; The microneedle-type multi-segment release composite particles release the ionic marine active factor and the amino acid in multiple stages as the structure disintegrates. A multi-stage release composite microparticle preparation method comprising an amino acid marine active factor peptide, comprising a chelation reaction step for electrically comparing a molar peptide to a plurality of ionic marine active factors Uniformly mixing and reacting at a temperature of 25 ° C to 35 ° C to form a multi-stage release composite particle having a hydrophilic particle space, wherein the peptide comprises a plurality of peptide ionic groups, a plurality of peptide hydrophilic groups, and a plurality a peptide hydrophobic group having a chelate framework of a peptide ionic group and an ionic ocean active factor; and a hydrogen bonding strengthening step for separating a plurality of selected from arginine, lysine, and glycine Amino acids of arginine, arginine, lysine, leucine, isoleucine, valine, aspartic acid, and combinations thereof are placed in the particulate space and provided by the amino acid The hydrogen bond interacts with the hydrophilic group of the peptide to strengthen the structure of the particle space, and the hydrophobic group of the peptide forms a hydrophobic characteristic region around the space of the particle to form a multi-stage release composite particle filled with amino acid; a separation step And the multi-stage release composite particle containing the amino acid-containing marine active factor peptide is obtained by using the dialysis extraction in the preparation system, wherein the multi-stage release composite particle comprises one for a multi-segment release peptide of the composite particle space; a plurality of ionic marine active factors for ionic chelation with the peptide; and an amino acid for enhancing the composite particle structure, wherein the peptide comprises a plurality of wins a peptide ionic group, a plurality of peptide hydrophilic groups and a plurality of peptide hydrophobic groups, wherein the peptide ionic group and the ionic marine active factor are used to chelate a particle space, the particle space being a hydrophilic space Used to fill the amino acid and enhance the peptide and ionic marine activity through the hydrogen bond provided by the amino acid and the hydrophilic group of the peptide The structure of the particle space of the chelation structure of the sex factor, the hydrophobic group of the peptide forms a hydrophobic characteristic region around the multi-stage release composite particle, and the multi-stage release composite particle will dissociate the ionic marine active factor with the structure disintegration The amino acid is released in multiple stages.