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TR200101930T2 - Nükleik asit oligomer hibritleşme olaylarının elektrokimyasal tayin metodu. - Google Patents

Nükleik asit oligomer hibritleşme olaylarının elektrokimyasal tayin metodu.

Info

Publication number
TR200101930T2
TR200101930T2 TR2001/01930T TR200101930T TR200101930T2 TR 200101930 T2 TR200101930 T2 TR 200101930T2 TR 2001/01930 T TR2001/01930 T TR 2001/01930T TR 200101930 T TR200101930 T TR 200101930T TR 200101930 T2 TR200101930 T2 TR 200101930T2
Authority
TR
Turkey
Prior art keywords
nucleic acid
acid oligomer
determination method
hybridization events
hybridization
Prior art date
Application number
TR2001/01930T
Other languages
English (en)
Inventor
Hartwich Gerhard
Original Assignee
Fritz Biochem Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26051373&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=TR200101930(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from DE19901761A external-priority patent/DE19901761A1/de
Application filed by Fritz Biochem Gmbh filed Critical Fritz Biochem Gmbh
Publication of TR200101930T2 publication Critical patent/TR200101930T2/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

Bulus nükleik asit oligomer hibritlesme olaylarinin elektrokimyasal tayin metoduyla ilgilidir. Bir ucundan iletken yüzeye bagli ve diger, serbest uçtan, redoks-aktif birime bagli olan DNA / RNA / PNA oligomer tek telleri, hibritizasyon matriksi (prob) gibi davranir. Tek telli oligonükleotitlerin bir bölümü test edilen oligonükleotit solüsyonu (hedef) ile hibritlenir, ilk basta olmayan veya çok zayif olarak bulunan redoks-aktif birim ile iletken yüzey arasindaki elektriksel iletisim sonuç olarak artmaktadir. Bu da hibritlesme olayinin, voltametri, amperometri ve iletkenlik ölçümü gibi elektrokimyasal metodlar kullanilarak tayinini mümkün kilar.
TR2001/01930T 1999-01-18 2000-01-07 Nükleik asit oligomer hibritleşme olaylarının elektrokimyasal tayin metodu. TR200101930T2 (tr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19901761A DE19901761A1 (de) 1999-01-18 1999-01-18 Verfahren zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen
DE19926457A DE19926457A1 (de) 1999-01-18 1999-04-29 Verfahren zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen

Publications (1)

Publication Number Publication Date
TR200101930T2 true TR200101930T2 (tr) 2002-06-21

Family

ID=26051373

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2001/01930T TR200101930T2 (tr) 1999-01-18 2000-01-07 Nükleik asit oligomer hibritleşme olaylarının elektrokimyasal tayin metodu.

Country Status (18)

Country Link
EP (1) EP1144685B1 (tr)
JP (1) JP2003521465A (tr)
CN (1) CN1352697A (tr)
AT (1) ATE238436T1 (tr)
AU (1) AU758063B2 (tr)
BR (1) BR0007571A (tr)
CA (1) CA2371938A1 (tr)
CZ (1) CZ20012503A3 (tr)
DE (1) DE50001859D1 (tr)
EA (1) EA003929B1 (tr)
ES (1) ES2198282T3 (tr)
HU (1) HUP0105170A3 (tr)
IL (1) IL144219A0 (tr)
MX (1) MXPA01007183A (tr)
NO (1) NO20013471L (tr)
SI (1) SI1144685T1 (tr)
TR (1) TR200101930T2 (tr)
WO (1) WO2000042217A2 (tr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19940749A1 (de) 1998-08-28 2000-05-18 Febit Ferrarius Biotech Gmbh Verfahren und Vorrichtung zur integrierten Synthese und Analyse von Polymeren
DE10049527A1 (de) * 2000-10-06 2001-09-06 Friz Biochem Gmbh Thermostabile,photoinduzierbar redoxaktive Einheit zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen
DE10051396A1 (de) 2000-10-17 2002-04-18 Febit Ferrarius Biotech Gmbh Verfahren und Vorrichtung zur integrierten Synthese und Analytbestimmung an einem Träger
US7291457B2 (en) * 2001-06-21 2007-11-06 The Regents Of The University Of California Electrochemical detection of mismatch nucleic acids
WO2003018834A2 (de) 2001-08-25 2003-03-06 Friz Biochem Gmbh Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen
DE20121631U1 (de) * 2001-11-09 2003-06-18 Friz Biochem GmbH, 82152 Planegg Molekulares elektronisches Bauelement zum Aufbau nanoelektronischer Schaltungen, molekulare elektronische Baugruppe und elektronische Schaltung
US7413859B2 (en) 2001-11-14 2008-08-19 Siemens Aktiengesellschaft Method and biosensors for detecting macromolecular biopolymers
DE10163836A1 (de) 2001-12-22 2003-07-10 Friz Biochem Gmbh Multifunktionales Reagenz zur Synthese von thiolmodifizierten Oligomeren
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
WO2005005952A2 (en) * 2003-05-13 2005-01-20 The Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection
US7741033B2 (en) 2003-05-13 2010-06-22 Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection
DE102004004882A1 (de) * 2004-01-30 2005-08-18 Dade Behring Marburg Gmbh Testsystem und Verfahren zum Nachweis von Analyten
DE102007044664B4 (de) 2007-09-18 2012-01-05 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verdrängungsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
CN102216762B (zh) 2008-09-02 2016-02-10 多伦多大学董事局 纳米结构的微电极以及集成所述微电极的生物传感器件
DE102009044795B3 (de) 2009-12-07 2011-04-07 Friz Biochem Gesellschaft Für Bioanalytik Mbh Kompetitionsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
WO2012097081A2 (en) 2011-01-11 2012-07-19 The Governing Council Of The University Of Toronto Protein detection method
ES2799422T3 (es) 2011-03-10 2020-12-17 General Atomics Dispositivos y métodos de diagnóstico y preparación de muestras
GB201120118D0 (en) * 2011-11-22 2012-01-04 Iti Scotland Ltd Detecting analytes
DE102011056606B3 (de) 2011-12-19 2013-01-03 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5457183A (en) * 1989-03-06 1995-10-10 Board Of Regents, The University Of Texas System Hydroxylated texaphyrins
DE4216696C2 (de) * 1992-04-10 1995-01-26 Deutsche Aerospace Verfahren und Vorrichtung zur Empfindlichkeits- und Selektivitätssteigerung bei Immuno-Assays, Molekül-Rezeptor-, DNA-komplementär-DNA- und Gast-Wirtsmolekül-Wechselwirkungs-Assays
US5312527A (en) * 1992-10-06 1994-05-17 Concordia University Voltammetric sequence-selective sensor for target polynucleotide sequences
US5824473A (en) * 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
DE19639169A1 (de) * 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
CA2270633A1 (en) * 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US6306584B1 (en) * 1997-01-21 2001-10-23 President And Fellows Of Harvard College Electronic-property probing of biological molecules at surfaces
US6221586B1 (en) * 1997-04-09 2001-04-24 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
DE19964220C2 (de) * 1998-11-23 2003-07-03 Friz Biochem Gmbh Verfahren zur Herstellung einer modifizierten leitfähigen Oberfläche

Also Published As

Publication number Publication date
EP1144685B1 (de) 2003-04-23
ATE238436T1 (de) 2003-05-15
WO2000042217A3 (de) 2000-11-30
DE50001859D1 (de) 2003-05-28
CA2371938A1 (en) 2000-07-20
WO2000042217A2 (de) 2000-07-20
AU2662700A (en) 2000-08-01
HUP0105170A2 (hu) 2002-05-29
ES2198282T3 (es) 2004-02-01
EP1144685A2 (de) 2001-10-17
EA200100672A1 (ru) 2002-02-28
SI1144685T1 (en) 2003-10-31
AU758063B2 (en) 2003-03-13
CN1352697A (zh) 2002-06-05
CZ20012503A3 (cs) 2002-01-16
NO20013471D0 (no) 2001-07-13
MXPA01007183A (es) 2002-05-06
HUP0105170A3 (en) 2005-03-29
BR0007571A (pt) 2001-11-27
EA003929B1 (ru) 2003-10-30
NO20013471L (no) 2001-09-13
IL144219A0 (en) 2002-05-23
JP2003521465A (ja) 2003-07-15

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