SU668939A1 - Method of separating aqueous culture medium - Google Patents

Description

(54) METHOD FOR CREATING WATER FOOD MEDIUM

one

The method relates to the microbiological industry, namely to the method of separating microbiological suspensions obtained by cultivating microorganisms on hydrocarbons. A method is known for separating the biomass of microorganisms from a suspension of microorganisms grown on hydrocarbons by decanting 1), to increase the degree of separation of aqueous culture medium into suspension of microorganisms air, which helps to improve flotation and the separation of microorganisms.

However, this method requires significant energy consumption for the introduction into the apparatus and the dispersion of air.

The proposed method allows to increase the output of the aqueous medium to the degree of thickening of the microbial suspension.

For this, the decantation process is carried out using contact surfaces in the form of SHEETS, sieves, Raschig rings or turning chips with a suspension load of up to 10 cm per 1 cm of the contact surface for 1 hour and a flow rate of suspension of 0.5-10 cm per minute.

The concentration of hydrocarbons in the suspension of microorganisms is maintained in the range of 5-80%.

Contact surfaces made of steel, ceramics or Teflon (polytetrafluoroethylene) are filled with a decanter apparatus, in which the separation of microbiological suspensions takes place.

The method is applicable to the separation of microbiological suspensions obtained by cultivating microorganisms on hydrocarbons, preferably petroleum distillates, boiling away from 100-400 ° C.

The method is carried out as follows. The microbiological suspension from the fermenter enters the decanter, where it is divided into two phases: light, containing biomass, hydrocarbons, air and some amount of aqueous medium, and heavy - aqueous medium. The light phase is decanted and then fed to the separation by known methods. In this case, an additional amount of aqueous medium is separated.

Claims (2)

Filling the decanter with contact surfaces allows one to increase the separation of the medium in the process of decanting by 1.5–2 times. In this way, a more concentrated light phase is obtained in decantation and the load on the subsequent thickening processes of microbiologists} of the suspension is reduced. The method is applicable to the separation of yeast and bacterial suspensions and their mixtures obtained by culturing microorganisms on hydrocarbons. I Example 1. The decanter, not filled with contact surfaces, continuously comes from the fermentor yeast suspension obtained by cultivating Condida guillierucondii yeast on oil distillates with boiling range of 220-360 C. Suspension contains 2% biomass and about 80% aqueous medium . In the decanter, the yeast suspension is divided into layers. The lower layer, a heavy phase consisting of an aqueous medium, continuously returns to the fermenter. The top layer is a light phase continuously fed to the separation. In the decanter that is not filled with contact surfaces, 41.6% of the water is separated i. The concentration of biomass increases from 2 to 3%. Example 2. A yeast slurry is continuously fed to a decanter filled with 0.5 m thick Teflon turning chips, similar in composition to Example 1. The amount of separated aqueous medium is 77.5%, the biomass concentrate increases from 2 to 5.5%. The claims of the invention: 1. A method for separating an aqueous culture medium from a hydrocarbon-containing suspension of microorganisms by decanting a suspension. so that, in order to increase the yield of the aqueous medium, the decantation of the suspension is carried out using contact surfaces in the form of sheets ,. Sieve, Raschiga rings or turning chips with a suspension load of up to 10 cm per 1 cm of contact surface for 1 hour or a suspension flow rate of 0.5-10 cm per minute. 2, the method according to p. I, about t and aa y u and so that the concentration of hydrocarbons in the suspension. ZIN of microorganisms is maintained within 5-80%. The sources of information taken into account during the examination. 1. The patent of England N 1177039, cl. From 6 F, 1970.