SU647006A1 - Sorbent for recovering protein from solution - Google Patents

Description

(54) SORBENT FOR ISOLATION OF PROTEIN. FROM SOLUTIONS

Heated hexachloride and the resulting hydrogen chloride at the reaction temperature, carried out the hydrolysis of chlorine in the products with water vapor and drying the sample at the same temperature. The sorbent has the following composition,%

Silica gel 75

Titanium oxide .6

Tungsten oxide 19 Sorption capacity of the sorbent is 4.84 mg / g

Example 2, Same as in example 1, but the content of titanium oxide in the original sample 10 weight. % per 1 g of sorbent. The composition of the sorbent,%

Silica gel 67

Titanium oxide 10

Tungsten oxide 19

The sorption capacity is 4.84 mg / g.

Example 3. Same as in example 1, but the content of titanium oxide in the original sample is 21 wt.% Per 1 g of sorbent. The composition of the sorbent,%:

Silica gel 50

Titanium oxide 20.0

Tungsten oxide 30.0

The sorption capacity is 4.65 mg / g.

Example 4. Same as in example 1, but the processing time with tungsten texachloride 1 h. The composition of the obtained sorbent,%:

; - Silica gel 81.0 Titanium oxide. 6.0

Tungsten oxide 13.0

The sorption capacity is 3.14 mg / g.

Example 6. Protein sorption from wash water on the resulting sorbent is carried out as follows.

A portion of the sorbent 0.25 g pour 5 ml of liquid containing 0.7-4.9 mg / m protein. Incubated at 0 ° C for 3 h, after which the solution is drained from the sample. . Wash before washing until there is no protein in the wash water. The washings and the slurry solution are mixed and the protein content is determined. The amount of sorbed protein is determined by the equality of the protein in the initial liquid and after sorption.

Content. components in the samples and the amount of protein sorbed on the resulting sorbents are given in the table.

As follows from the table, the additional introduction of tungsten oxide into silica gel modified with titanium oxide allows extracting protein from the solution by 9%, which is more than 2 times higher than known sorbents. In addition, the proposed sorbent is synthesized and can be repeatedly used, since it is easily regenerated by phosphate buffer.