SU303877A1 - The method of obtaining arabogalactan - Google Patents

Description

The present invention relates to a method for obtaining high purity arabogalactan and can be used in the field of organic chemistry.

A method of producing arabogalactan from aqueous extracts of larch wood passed through activated carbon is known in order to free it from staining reddish-brown impurities. However, activated charcoal does not provide complete removal of coloring impurities (positive reaction with FolinDenisa reagent), and arabogalactan has a cream shade. In addition, part of the arabogalactan is absorbed by activated carbon, which reduces the yield of the product.

In order to obtain high quality arabogalactan, it is proposed to obtain it from aqueous extracts of larch wood, passed through a polyamide powder.

The proposed method allows to obtain high quality arabogalactan: white, with a total absence of coloring impurities, with a reduced content of uronic acids and ash.

Example 1. Sawdust wood of Siberian larch (0.25-1.0 mm) is extracted with water for 1 hour at 80 ° C. The aqueous extract is filtered and 50 ml of the filtrate containing 0.3 g of dry matter (from

0.268 g of arabogalactan), is passed through a 0.8 cm column filled with polyamide (nylon) powder (0.5 g). Further washing of the column with 200 ml of water with four times the amount leads to the complete leaching of arabogalactan; the amount of arabogalactan in the eluate is 0.265 g or 99.8% of the arabogalactan in the original extract. Arabogalactan is a white powder with a reduced ash content of 0.64% and a content of uronic acids of 0.2%. At the same time, the ash content of arabogalactan, usually extracted from the extract, is 0.81% and the content of uronic acids is 0.534%. The purity of the obtained product is proved by the absence of absorption in UV light in the range of 285-290 by them, which is characteristic of coloring phenolic impurities.

The regeneration of the polyamide powder is carried out by subsequent washing with its water-acetone mixture (35:65 by volume), 0.5 n. NaOH, 2% acetic acid II water. Regenerated polpamid suitable for further use.

Example 2. Sawdust of larch wood with a size of 0.25-1.0 mm is treated with water at a temperature of boiling water bath for 2 hours, the extract is filtered and 50 ml of the extract containing 0.46 g

dry matter (of which 0.422 g of arabogalactan), is passed through a column with a polyamnd powder (a layer of polyamide 0.8X2.5 cm). Washing the column with 200 ml of water provides up to 0.42 g of arabogalactapa or 99.5% of the content of the initial extract.

Arabogalactan isolated from white eluate contains 0.2% ash and 1.2% uronic acids. Regular arabogalactan contains 0.34% ash, 2.8% uronic acids.

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The method of obtaining arabogalactan by treating plant material, for example, leaflets, is fed, followed by passing the obtained solution through an absorber, about tl and h: o y and so that, in order to improve the quality of the target product, polyamide powder is taken as absorber.