SG11201810531TA - Ligand identification by co-fractionation - Google Patents
Ligand identification by co-fractionationInfo
- Publication number
- SG11201810531TA SG11201810531TA SG11201810531TA SG11201810531TA SG11201810531TA SG 11201810531T A SG11201810531T A SG 11201810531TA SG 11201810531T A SG11201810531T A SG 11201810531TA SG 11201810531T A SG11201810531T A SG 11201810531TA SG 11201810531T A SG11201810531T A SG 11201810531TA
- Authority
- SG
- Singapore
- Prior art keywords
- ligands
- international
- potsdam
- macromolecules
- pct
- Prior art date
Links
- 239000003446 ligand Substances 0.000 title abstract 9
- 238000005194 fractionation Methods 0.000 title abstract 3
- 229920002521 macromolecule Polymers 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 239000011534 wash buffer Substances 0.000 abstract 3
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 101100172879 Caenorhabditis elegans sec-5 gene Proteins 0.000 abstract 1
- BZLVMXJERCGZMT-UHFFFAOYSA-N Methyl tert-butyl ether Chemical compound COC(C)(C)C BZLVMXJERCGZMT-UHFFFAOYSA-N 0.000 abstract 1
- 238000009614 chemical analysis method Methods 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 150000002632 lipids Chemical class 0.000 abstract 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 239000008188 pellet Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
- G01N24/08—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance
- G01N24/087—Structure determination of a chemical compound, e.g. of a biomolecule such as a protein
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
- G01N24/08—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance
- G01N24/088—Assessment or manipulation of a chemical or biochemical reaction, e.g. verification whether a chemical reaction occurred or whether a ligand binds to a receptor in drug screening or assessing reaction kinetics
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- High Energy & Nuclear Physics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Rehabilitation Therapy (AREA)
- Rheumatology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Electrochemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
AgurelMaterial 2.Lysis 3.Fractionation --10. 3452g 10' 128812g 45' 4b SEC 5. MTBE & LC/MS SECedutionvolum. 444 4 1 Fmdiong 44 4 W O 20 17 / 207 460 Al Input • . Lipids (upper phase) Polar (lower phase) Proteins (pellet) Time (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 07 December 2017 (07.12.2017) WIP0 I PCT omit VIII inn Holonmomiloolioluom (10) International Publication Number WO 2017/207460 Al (51) International Patent Classification: GO1N 33/564 (2006.01) GO1N 33/68 (2006.01) (21) International Application Number: PCT/EP2017/062841 (22) International Filing Date: 29 May 2017 (29.05.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 16172000.8 30 May 2016 (30.05.2016) EP (71) Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V. [DE/DE]; Hofgartenstr. 8, 80539 Miinchen (DE). (72) Inventors: SKIRYCZ, Aleksandra; Am Alten Mortel- werk 10, 14469 Potsdam (DE). KIERSZNIOWSKA, Sylwia; Glolmer Fichten 32, 14476 Potsdam (DE). VEYEL, Daniel; Gartenstr. 9, 14482 Potsdam (DE). WILLMITZER, Lothar; Waldwinkel 27, 14532 Klein- machnow (DE). CHODASIEWICZ, Monika; Storchen- hof 5, 14476 Potsdam (DE). (74) Agent: VOSSIUS & PARTNER (NO 31); SiebertstraBe 3, 81675 Miinchen (DE). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 2 I (3)) (54) Title: LIGAND IDENTIFICATION BY CO-FRACTIONATION (57) : This invention relates to a method of determining ligands of macromolecules, said method comprising or consisting of (a) subjecting a sample comprising (i) complexes formed by said macromolecules and said ligands and (ii) unbound ligands to a method which separates said complexes from said un- bound ligands; (b) releasing ligands from complexes obtained in step (a); and (c) subjecting the released ligands obtained in step (b) to a chemical analysis method, thereby determining said ligands of said macromolecules. 4a Size Filtration 10. 3452g 30' 3452g 15' 10' 3452g 15' Input Wash Buffer Wash Buffer i Wash Buffer // ' C ' ,7- Flow Wash 100°c Elute
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16172000 | 2016-05-30 | ||
| PCT/EP2017/062841 WO2017207460A1 (en) | 2016-05-30 | 2017-05-29 | Ligand identification by co-fractionation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201810531TA true SG11201810531TA (en) | 2018-12-28 |
Family
ID=56119308
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201810531TA SG11201810531TA (en) | 2016-05-30 | 2017-05-29 | Ligand identification by co-fractionation |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US10976310B2 (en) |
| EP (1) | EP3465207B1 (en) |
| KR (1) | KR102346785B1 (en) |
| CN (1) | CN109219750B (en) |
| AU (1) | AU2017272493B2 (en) |
| BR (1) | BR112018074856A2 (en) |
| CA (1) | CA3025711A1 (en) |
| DK (1) | DK3465207T3 (en) |
| ES (1) | ES2899110T3 (en) |
| HU (1) | HUE056359T2 (en) |
| LT (1) | LT3465207T (en) |
| PL (1) | PL3465207T3 (en) |
| PT (1) | PT3465207T (en) |
| SG (1) | SG11201810531TA (en) |
| SI (1) | SI3465207T1 (en) |
| WO (1) | WO2017207460A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210124374A (en) | 2019-02-08 | 2021-10-14 | 듀포인트 테라퓨틱스, 인크. | Methods for characterizing condensate-associated properties of compounds and uses thereof |
| EP4031876A1 (en) * | 2019-09-18 | 2022-07-27 | Dewpoint Therapeutics, Inc. | Methods of screening for condensate-associated specificity and uses thereof |
| JP7327096B2 (en) * | 2019-11-13 | 2023-08-16 | 株式会社島津製作所 | Molecular separation method |
| CN113528497B (en) * | 2021-07-22 | 2022-06-24 | 中国林业科学研究院林业研究所 | Application of FBA8 gene or FBA8 protein in the preparation of reagents with phosphotransfer activity and/or proteolytic activity |
| WO2024112940A1 (en) * | 2022-11-26 | 2024-05-30 | Enveda Therapeutics, Inc. | Methods and systems for identifying compounds for forming, stabilizing or disrupting molecular complexes |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4459359A (en) * | 1981-11-19 | 1984-07-10 | New York Blood Center, Inc. | Sensitive immunoassays of antigens or antibodies sequestered within immune complexes |
| AU774776B2 (en) * | 1999-04-15 | 2004-07-08 | University Of Virginia Patent Foundation | Proteome mining |
| WO2004019967A1 (en) | 2002-08-14 | 2004-03-11 | Blanchette Rockefeller Neuroscience Institute | Method for isolating subpopulations of proteins that engage in protein-protein interactions |
| WO2006015796A1 (en) * | 2004-08-09 | 2006-02-16 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Method for high-throughput screening of test molecules binding to target molecules |
| US20150105280A1 (en) * | 2010-03-10 | 2015-04-16 | Perfinity Biosciences, Inc. | Selector based recognition and quantification system and method for multiple analytes in a single analysis |
| EP2922861A4 (en) * | 2012-11-26 | 2016-09-14 | Caris Life Sciences Switzerland Holdings Gmbh | Biomarker compositions and methods |
-
2017
- 2017-05-29 AU AU2017272493A patent/AU2017272493B2/en active Active
- 2017-05-29 ES ES17725620T patent/ES2899110T3/en active Active
- 2017-05-29 LT LTEPPCT/EP2017/062841T patent/LT3465207T/en unknown
- 2017-05-29 CN CN201780034328.7A patent/CN109219750B/en active Active
- 2017-05-29 PT PT177256203T patent/PT3465207T/en unknown
- 2017-05-29 PL PL17725620T patent/PL3465207T3/en unknown
- 2017-05-29 DK DK17725620.3T patent/DK3465207T3/en active
- 2017-05-29 BR BR112018074856-0A patent/BR112018074856A2/en active Search and Examination
- 2017-05-29 KR KR1020187038204A patent/KR102346785B1/en active Active
- 2017-05-29 SG SG11201810531TA patent/SG11201810531TA/en unknown
- 2017-05-29 SI SI201730986T patent/SI3465207T1/en unknown
- 2017-05-29 HU HUE17725620A patent/HUE056359T2/en unknown
- 2017-05-29 EP EP17725620.3A patent/EP3465207B1/en active Active
- 2017-05-29 US US16/304,182 patent/US10976310B2/en active Active
- 2017-05-29 CA CA3025711A patent/CA3025711A1/en active Pending
- 2017-05-29 WO PCT/EP2017/062841 patent/WO2017207460A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| KR102346785B1 (en) | 2022-01-04 |
| PT3465207T (en) | 2021-11-30 |
| CA3025711A1 (en) | 2017-12-07 |
| LT3465207T (en) | 2021-12-10 |
| HUE056359T2 (en) | 2022-02-28 |
| CN109219750A (en) | 2019-01-15 |
| ES2899110T3 (en) | 2022-03-10 |
| US20190361012A1 (en) | 2019-11-28 |
| KR20190013999A (en) | 2019-02-11 |
| SI3465207T1 (en) | 2022-01-31 |
| US10976310B2 (en) | 2021-04-13 |
| EP3465207B1 (en) | 2021-09-01 |
| CN109219750B (en) | 2022-05-31 |
| AU2017272493B2 (en) | 2021-07-22 |
| EP3465207A1 (en) | 2019-04-10 |
| PL3465207T3 (en) | 2022-01-17 |
| DK3465207T3 (en) | 2021-11-15 |
| WO2017207460A1 (en) | 2017-12-07 |
| AU2017272493A1 (en) | 2018-12-20 |
| BR112018074856A2 (en) | 2019-03-06 |
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