SG11201701245QA - Methods for increasing cas9-mediated engineering efficiency - Google Patents
Methods for increasing cas9-mediated engineering efficiencyInfo
- Publication number
- SG11201701245QA SG11201701245QA SG11201701245QA SG11201701245QA SG11201701245QA SG 11201701245Q A SG11201701245Q A SG 11201701245QA SG 11201701245Q A SG11201701245Q A SG 11201701245QA SG 11201701245Q A SG11201701245Q A SG 11201701245QA SG 11201701245Q A SG11201701245Q A SG 11201701245QA
- Authority
- SG
- Singapore
- Prior art keywords
- methods
- engineering efficiency
- mediated engineering
- cas9
- increasing
- Prior art date
Links
- 101150038500 cas9 gene Proteins 0.000 title 1
- 230000001404 mediated effect Effects 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/30—Endoribonucleases active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters (3.1.30)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462042358P | 2014-08-27 | 2014-08-27 | |
| US201462047495P | 2014-09-08 | 2014-09-08 | |
| PCT/US2015/047046 WO2016033246A1 (en) | 2014-08-27 | 2015-08-26 | Methods for increasing cas9-mediated engineering efficiency |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201701245QA true SG11201701245QA (en) | 2017-03-30 |
Family
ID=54140655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201701245QA SG11201701245QA (en) | 2014-08-27 | 2015-08-26 | Methods for increasing cas9-mediated engineering efficiency |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US9970030B2 (en) |
| EP (1) | EP3186376B1 (en) |
| AU (1) | AU2015308910B2 (en) |
| CA (1) | CA2959070C (en) |
| DK (1) | DK3186376T3 (en) |
| ES (1) | ES2730378T3 (en) |
| GB (1) | GB2544001A (en) |
| IL (1) | IL250700B (en) |
| NZ (1) | NZ728437A (en) |
| SG (1) | SG11201701245QA (en) |
| WO (1) | WO2016033246A1 (en) |
Families Citing this family (73)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| HRP20181648T1 (en) | 2013-04-16 | 2019-01-25 | Regeneron Pharmaceuticals, Inc. | TARGET GENOMIC TARGET MODIFICATION |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| MX388127B (en) | 2013-12-11 | 2025-03-19 | Regeneron Pharma | METHODS AND COMPOSITIONS FOR THE TARGETED MODIFICATION OF A GENOME. |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| ES2731437T3 (en) | 2014-11-21 | 2019-11-15 | Regeneron Pharma | Methods and compositions for directed genetic modification through the use of guide RNA pairs |
| KR102763527B1 (en) | 2014-12-03 | 2025-02-05 | 애질런트 테크놀로지스, 인크. | Guide rna with chemical modifications |
| US12359197B2 (en) | 2014-12-12 | 2025-07-15 | Etagen Pharma, Inc. | Compositions and methods for editing nucleic acids in cells utilizing oligonucleotides |
| ES2884838T3 (en) | 2015-04-06 | 2021-12-13 | Univ Leland Stanford Junior | Chemically modified guide RNA for CRISPR / CAS-mediated gene regulation |
| US10167457B2 (en) | 2015-10-23 | 2019-01-01 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| US11905521B2 (en) * | 2015-11-17 | 2024-02-20 | The Chinese University Of Hong Kong | Methods and systems for targeted gene manipulation |
| EP3420080B1 (en) * | 2016-02-22 | 2019-08-21 | Caribou Biosciences, Inc. | Methods for modulating dna repair outcomes |
| WO2017155408A1 (en) * | 2016-03-11 | 2017-09-14 | Erasmus University Medical Center Rotterdam | Improved crispr-cas9 genome editing tool |
| EP3219799A1 (en) | 2016-03-17 | 2017-09-20 | IMBA-Institut für Molekulare Biotechnologie GmbH | Conditional crispr sgrna expression |
| EP4495235A3 (en) * | 2016-04-13 | 2025-05-21 | Editas Medicine, Inc. | Grna fusion molecules, gene editing systems, and methods of use thereof |
| EP3452055A4 (en) * | 2016-05-06 | 2019-11-06 | Tod M. Woolf | IMPROVED METHODS OF GENOME EDITING WITH AND WITHOUT PROGRAMMABLE NUCLEASES |
| CN113831407B (en) | 2016-05-20 | 2024-06-11 | 瑞泽恩制药公司 | Methods for breaking immune tolerance using multiple guide RNAs |
| GB2582731B8 (en) * | 2016-06-02 | 2021-10-27 | Sigma Aldrich Co Llc | Using programmable DNA binding proteins to enhance targeted genome modification |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| WO2017214632A2 (en) * | 2016-06-10 | 2017-12-14 | University Of Wyoming | Recombinant insect vectors and methods of use |
| JP6855037B2 (en) * | 2016-07-19 | 2021-04-07 | 国立大学法人大阪大学 | Genome editing method |
| KR20250103795A (en) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | Adenosine nucleobase editors and uses thereof |
| EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11466283B2 (en) | 2016-08-18 | 2022-10-11 | Canopy Growth Corporation | Plants and methods for increasing and decreasing synthesis of cannabinoids |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| WO2018129368A2 (en) | 2017-01-06 | 2018-07-12 | Editas Medicine, Inc. | Methods of assessing nuclease cleavage |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| WO2018170015A1 (en) * | 2017-03-14 | 2018-09-20 | The Regents Of The University Of California | Engineering crispr cas9 immune stealth |
| BR112019019655A2 (en) | 2017-03-23 | 2020-04-22 | Harvard College | nucleobase editors comprising nucleic acid programmable dna binding proteins |
| US11680262B2 (en) * | 2017-03-30 | 2023-06-20 | Kyoto University | Method for inducing exon skipping by genome editing |
| US12173309B2 (en) * | 2017-04-10 | 2024-12-24 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Compounds for increasing genome editing efficiency |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| CN111315889A (en) * | 2017-09-08 | 2020-06-19 | 生命技术公司 | Methods and compositions for enhancing homologous recombination |
| AU2018352592C1 (en) | 2017-10-16 | 2025-09-25 | Beam Therapeutics, Inc. | Uses of adenosine base editors |
| CN111448313A (en) * | 2017-11-16 | 2020-07-24 | 阿斯利康(瑞典)有限公司 | Compositions and methods for improving the effectiveness of Cas9-based knock-in strategies |
| WO2019099982A1 (en) * | 2017-11-17 | 2019-05-23 | The Johns Hopkins University | Compositions and methods for efficient genome editing |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| WO2019222545A1 (en) | 2018-05-16 | 2019-11-21 | Synthego Corporation | Methods and systems for guide rna design and use |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| ES3023563T3 (en) | 2018-10-16 | 2025-06-02 | Blueallele Corp | Methods for targeted insertion of dna in genes |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| CN114127285B (en) | 2019-03-19 | 2024-09-10 | 布罗德研究所股份有限公司 | Methods and compositions for editing nucleotide sequences |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| WO2020243365A1 (en) * | 2019-05-29 | 2020-12-03 | Monsanto Technology Llc | Directed genome engineering using enhanced targeted editing technologies |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| DE112021002672T5 (en) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EDIT BOTH STRANDS SIMULTANEOUSLY OF A DOUBLE STRANDED NUCLEOTIDE TARGET SEQUENCE |
| GB202007943D0 (en) * | 2020-05-27 | 2020-07-08 | Snipr Biome Aps | Products & methods |
| WO2022060707A1 (en) * | 2020-09-15 | 2022-03-24 | Rutgers, The State University Of New Jersey | Systems for gene editing and methods of use thereof |
| AU2022216614A1 (en) | 2021-02-05 | 2023-02-23 | Christiana Care Gene Editing Institute, Inc. | Methods of and compositions for reducing gene expression and/or activity |
| US20220325338A1 (en) * | 2021-04-02 | 2022-10-13 | The Regents Of The University Of California | Systems and methods for performing and measuring homologous chromosome template repair |
| WO2022256448A2 (en) * | 2021-06-01 | 2022-12-08 | Artisan Development Labs, Inc. | Compositions and methods for targeting, editing, or modifying genes |
| CN115466780B (en) * | 2021-06-10 | 2025-08-15 | 北京大学 | Method for researching conformational change of Cas9 protein when recognizing target gene locus |
| US11884915B2 (en) | 2021-09-10 | 2024-01-30 | Agilent Technologies, Inc. | Guide RNAs with chemical modification for prime editing |
| CN113801852B (en) * | 2021-10-18 | 2023-08-18 | 齐齐哈尔医学院 | A GPD1L-deleted human embryonic stem cell line and its construction method and application |
| EP4473108A1 (en) * | 2022-02-02 | 2024-12-11 | Biotalys NV | Methods for genome editing |
| CN114703174B (en) * | 2022-04-12 | 2023-10-24 | 中国科学院海洋研究所 | CRISPR/Cas9 gene knockout method for rapidly obtaining genotype and phenotype mutation and application thereof |
| CN120077129A (en) * | 2022-10-21 | 2025-05-30 | 沃奇酶科基因组学公司 | Methods and compositions for sequencing library normalization |
| WO2024092217A1 (en) * | 2022-10-27 | 2024-05-02 | The Trustees Of Columbia University In The City Of New York | Systems and methods for gene insertions |
| CN116286975A (en) * | 2023-02-21 | 2023-06-23 | 浙江大学 | A dCas9-based c-NHEJ fixed-site inhibition system and its application |
| CN117126828A (en) * | 2023-09-15 | 2023-11-28 | 通用生物(安徽)股份有限公司 | Method for improving in-vitro enzyme digestion efficiency of Cas9 |
Family Cites Families (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1152058A1 (en) * | 2000-05-03 | 2001-11-07 | Institut Curie | Methods and compositions for effecting homologous recombination |
| US10087431B2 (en) | 2010-03-10 | 2018-10-02 | The Regents Of The University Of California | Methods of generating nucleic acid fragments |
| BR112012028805A2 (en) | 2010-05-10 | 2019-09-24 | The Regents Of The Univ Of California E Nereus Pharmaceuticals Inc | endoribonuclease compositions and methods of use thereof. |
| WO2013049330A1 (en) | 2011-09-28 | 2013-04-04 | The Regents Of The University Of California | Synthetic transcriptional control elements and methods of generating and using such elements |
| US9895451B2 (en) | 2011-12-02 | 2018-02-20 | Yale University | Formulations for targeted release of agents to low pH tissue environments or cellular compartments and methods of use thereof |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| WO2013122816A1 (en) | 2012-02-13 | 2013-08-22 | The Regents Of The University Of California | Methods for genome-wide screening and construction of genetic interaction maps |
| RU2639277C2 (en) | 2012-02-29 | 2017-12-20 | Сангамо Байосайенсиз, Инк. | Methods and compositions for huntington disease treatment |
| CN104364394B (en) | 2012-03-17 | 2019-02-22 | 加州大学评议会 | Rapid diagnosis and individualized treatment of acne |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
| PT3241902T (en) | 2012-05-25 | 2018-05-28 | Univ California | METHODS AND COMPOSITIONS FOR MODIFICATION OF TARGETED TARGET DNA BY RNA AND FOR MODULATION DIRECTED BY TRANSCRIPTION RNA |
| WO2013188638A2 (en) | 2012-06-15 | 2013-12-19 | The Regents Of The University Of California | Endoribonucleases and methods of use thereof |
| KR102530118B1 (en) | 2012-07-25 | 2023-05-08 | 더 브로드 인스티튜트, 인코퍼레이티드 | Inducible dna binding proteins and genome perturbation tools and applications thereof |
| WO2014022702A2 (en) | 2012-08-03 | 2014-02-06 | The Regents Of The University Of California | Methods and compositions for controlling gene expression by rna processing |
| CA2882499C (en) | 2012-08-29 | 2023-09-26 | Sangamo Biosciences, Inc. | Cells genetically modified within the bcl11a gene by a nuclease and various aspects related thereto |
| UA118090C2 (en) | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks |
| WO2014065596A1 (en) * | 2012-10-23 | 2014-05-01 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| CA2890160A1 (en) | 2012-10-31 | 2014-05-08 | Cellectis | Coupling herbicide resistance with targeted insertion of transgenes in plants |
| US10272163B2 (en) | 2012-12-07 | 2019-04-30 | The Regents Of The University Of California | Factor VIII mutation repair and tolerance induction |
| WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
| ES2553782T3 (en) | 2012-12-12 | 2015-12-11 | The Broad Institute, Inc. | Systems engineering, methods and guide compositions optimized for sequence manipulation |
| KR20150105634A (en) | 2012-12-12 | 2015-09-17 | 더 브로드 인스티튜트, 인코퍼레이티드 | Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation |
| EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| DK2784162T3 (en) | 2012-12-12 | 2015-07-13 | Broad Inst Inc | Design of systems, methods and optimized control manipulations for sequence manipulation |
| EP4234696A3 (en) | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
| SG10201707569YA (en) | 2012-12-12 | 2017-10-30 | Broad Inst Inc | Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| WO2014093655A2 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
| DK3553174T3 (en) * | 2012-12-17 | 2025-08-04 | Harvard College | RNA-GUIDED MODIFICATION OF THE HUMAN GENOME |
| US10513698B2 (en) | 2012-12-21 | 2019-12-24 | Cellectis | Potatoes with reduced cold-induced sweetening |
| CN103981147B (en) | 2013-02-08 | 2017-11-10 | 中国科学院上海生命科学研究院 | A kind of new method for preparing hepatic parenchymal cells |
| US20140288421A1 (en) | 2013-03-12 | 2014-09-25 | The Regents Of The University Of California | Gas vesicle ultrasound contrast agents and methods of using the same |
| US20140288411A1 (en) | 2013-03-12 | 2014-09-25 | The Regents Of The University Of California | Gas vesicle magnetic resonance imaging contrast agents and methods of using the same |
| NZ712727A (en) | 2013-03-14 | 2017-05-26 | Caribou Biosciences Inc | Compositions and methods of nucleic acid-targeting nucleic acids |
| US11332719B2 (en) | 2013-03-15 | 2022-05-17 | The Broad Institute, Inc. | Recombinant virus and preparations thereof |
| AU2014235968B2 (en) | 2013-03-21 | 2018-05-24 | Ospedale San Raffaele Srl | Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases |
| CN115261411A (en) | 2013-04-04 | 2022-11-01 | 哈佛学院校长同事会 | Therapeutic uses of genome editing with CRISPR/Cas systems |
| US9902973B2 (en) | 2013-04-11 | 2018-02-27 | Caribou Biosciences, Inc. | Methods of modifying a target nucleic acid with an argonaute |
| CA2910489A1 (en) | 2013-05-15 | 2014-11-20 | Sangamo Biosciences, Inc. | Methods and compositions for treatment of a genetic condition |
| US20140349405A1 (en) | 2013-05-22 | 2014-11-27 | Wisconsin Alumni Research Foundation | Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis |
| WO2014191518A1 (en) | 2013-05-29 | 2014-12-04 | Cellectis | A method for producing precise dna cleavage using cas9 nickase activity |
| MX385330B (en) | 2013-06-04 | 2025-03-18 | Harvard College | RIBONUCLEIC ACID-GUIDED TRANSCRIPTIONAL REGULATION. |
| US9267135B2 (en) | 2013-06-04 | 2016-02-23 | President And Fellows Of Harvard College | RNA-guided transcriptional regulation |
| WO2015031619A1 (en) | 2013-08-28 | 2015-03-05 | Sangamo Biosciences, Inc. | Compositions for linking dna-binding domains and cleavage domains |
| US20160298096A1 (en) | 2013-11-18 | 2016-10-13 | Crispr Therapeutics Ag | Crispr-cas system materials and methods |
| AU2014363991B2 (en) | 2013-12-09 | 2020-08-27 | Sangamo Therapeutics, Inc. | Methods and compositions for genome engineering |
| JP2017501149A (en) | 2013-12-12 | 2017-01-12 | ザ・ブロード・インスティテュート・インコーポレイテッド | Delivery, use and therapeutic applications of CRISPR-CAS systems and compositions for targeting disorders and diseases using particle delivery components |
| US20150191744A1 (en) | 2013-12-17 | 2015-07-09 | University Of Massachusetts | Cas9 effector-mediated regulation of transcription, differentiation and gene editing/labeling |
| US10774338B2 (en) | 2014-01-16 | 2020-09-15 | The Regents Of The University Of California | Generation of heritable chimeric plant traits |
| DK3102673T3 (en) | 2014-02-03 | 2020-07-06 | Sangamo Therapeutics Inc | METHODS AND COMPOSITIONS FOR THE TREATMENT OF BETA-TALASSEMI |
| WO2015143046A2 (en) | 2014-03-18 | 2015-09-24 | Sangamo Biosciences, Inc. | Methods and compositions for regulation of zinc finger protein expression |
| WO2015171932A1 (en) | 2014-05-08 | 2015-11-12 | Sangamo Biosciences, Inc. | Methods and compositions for treating huntington's disease |
| US9944933B2 (en) * | 2014-07-17 | 2018-04-17 | Georgia Tech Research Corporation | Aptamer-guided gene targeting |
| IL287561B2 (en) * | 2014-10-01 | 2024-03-01 | Massachusetts Gen Hospital | Methods for increasing the efficiency of nuclease-induced homology-directed repair |
-
2015
- 2015-08-26 SG SG11201701245QA patent/SG11201701245QA/en unknown
- 2015-08-26 AU AU2015308910A patent/AU2015308910B2/en active Active
- 2015-08-26 NZ NZ728437A patent/NZ728437A/en unknown
- 2015-08-26 EP EP15763698.6A patent/EP3186376B1/en active Active
- 2015-08-26 DK DK15763698.6T patent/DK3186376T3/en active
- 2015-08-26 ES ES15763698T patent/ES2730378T3/en active Active
- 2015-08-26 CA CA2959070A patent/CA2959070C/en active Active
- 2015-08-26 GB GB1703026.3A patent/GB2544001A/en not_active Withdrawn
- 2015-08-26 WO PCT/US2015/047046 patent/WO2016033246A1/en not_active Ceased
- 2015-08-26 US US14/836,753 patent/US9970030B2/en active Active
-
2017
- 2017-02-21 IL IL250700A patent/IL250700B/en active IP Right Grant
-
2018
- 2018-02-02 US US15/887,893 patent/US20180171360A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2015308910B2 (en) | 2017-12-07 |
| GB2544001A (en) | 2017-05-03 |
| IL250700B (en) | 2020-10-29 |
| US20160257973A1 (en) | 2016-09-08 |
| GB201703026D0 (en) | 2017-04-12 |
| EP3186376B1 (en) | 2019-03-20 |
| US9970030B2 (en) | 2018-05-15 |
| NZ728437A (en) | 2018-02-23 |
| EP3186376A1 (en) | 2017-07-05 |
| DK3186376T3 (en) | 2019-05-06 |
| US20180171360A1 (en) | 2018-06-21 |
| AU2015308910A1 (en) | 2017-02-09 |
| WO2016033246A1 (en) | 2016-03-03 |
| ES2730378T3 (en) | 2019-11-11 |
| CA2959070A1 (en) | 2016-03-03 |
| CA2959070C (en) | 2020-11-10 |
| IL250700A0 (en) | 2017-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL250700A0 (en) | Methods for increasing cas9-mediated engineering efficiency | |
| GB201406151D0 (en) | Method | |
| GB201409558D0 (en) | Method | |
| GB201406155D0 (en) | Method | |
| GB201408119D0 (en) | Method | |
| GB201413240D0 (en) | Method | |
| GB201414496D0 (en) | Method | |
| GB201403470D0 (en) | Method | |
| GB201410022D0 (en) | Method | |
| GB201410101D0 (en) | Method | |
| GB201412802D0 (en) | Method | |
| GB201411500D0 (en) | Method | |
| GB201416849D0 (en) | Method | |
| GB201416675D0 (en) | Method | |
| GB201409234D0 (en) | Method | |
| GB201409128D0 (en) | Method | |
| GB201414181D0 (en) | Method | |
| GB201412736D0 (en) | Method | |
| GB201412226D0 (en) | Method | |
| GB201409101D0 (en) | Method | |
| GB201413929D0 (en) | Method | |
| GB201413612D0 (en) | Method | |
| GB201408649D0 (en) | Method | |
| GB201407971D0 (en) | Method | |
| GB201409206D0 (en) | Block turbine |