SG11201500655VA - Method for isolating rna including small rna with high yield - Google Patents
Method for isolating rna including small rna with high yieldInfo
- Publication number
- SG11201500655VA SG11201500655VA SG11201500655VA SG11201500655VA SG11201500655VA SG 11201500655V A SG11201500655V A SG 11201500655VA SG 11201500655V A SG11201500655V A SG 11201500655VA SG 11201500655V A SG11201500655V A SG 11201500655VA SG 11201500655V A SG11201500655V A SG 11201500655VA
- Authority
- SG
- Singapore
- Prior art keywords
- rna
- high yield
- including small
- isolating
- small rna
- Prior art date
Links
- 108091032973 (ribonucleotides)n+m Proteins 0.000 title 1
- 108091032955 Bacterial small RNA Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261696244P | 2012-09-03 | 2012-09-03 | |
| EP12182741 | 2012-09-03 | ||
| PCT/EP2013/068197 WO2014033326A1 (fr) | 2012-09-03 | 2013-09-03 | Méthode d'isolement d'arn comprenant un petit arn avec un rendement élevé |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201500655VA true SG11201500655VA (en) | 2015-05-28 |
Family
ID=46796430
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201500655VA SG11201500655VA (en) | 2012-09-03 | 2013-09-03 | Method for isolating rna including small rna with high yield |
| SG10201701747YA SG10201701747YA (en) | 2012-09-03 | 2013-09-03 | Method for isolating rna including small rna with high yield |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201701747YA SG10201701747YA (en) | 2012-09-03 | 2013-09-03 | Method for isolating rna including small rna with high yield |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US10329553B2 (fr) |
| EP (1) | EP2893015B1 (fr) |
| JP (1) | JP6440616B2 (fr) |
| CN (1) | CN104603267B (fr) |
| AU (1) | AU2013310861B2 (fr) |
| CA (1) | CA2880136C (fr) |
| SG (2) | SG11201500655VA (fr) |
| WO (1) | WO2014033326A1 (fr) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050059024A1 (en) * | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| EP3169780B1 (fr) * | 2014-07-17 | 2020-02-12 | Qiagen GmbH | Procédé d'isolement d'arn ayant un rendement élevé |
| JP2017537616A (ja) * | 2014-11-14 | 2017-12-21 | コーニング インコーポレイテッド | インビトロ転写後のｒｎａ精製のための方法及びキット |
| US11111488B2 (en) | 2015-10-28 | 2021-09-07 | Mirnagreen S.R.L. | Methods for extracting bioactive small RNAs from plants and mushrooms |
| CN105385681B (zh) * | 2015-12-29 | 2018-07-17 | 中国海洋大学 | 一种用于鲆鲽鱼类血液rna提取的裂解液 |
| US20180135040A1 (en) | 2016-02-16 | 2018-05-17 | Life Magnetics, Inc. | Methods for separating nucleic acids with graphene coated magnetic beads |
| WO2018156906A1 (fr) * | 2017-02-27 | 2018-08-30 | miDiagnostics NV | Système et procédé de purification et d'amplification d'acides nucléiques |
| JP7285257B2 (ja) * | 2018-07-06 | 2023-06-01 | 社会医療法人大雄会 | 小型ｒｎａの抽出効率を改善するための組成物および方法 |
| US20240052336A1 (en) * | 2019-10-01 | 2024-02-15 | Showa Denko Materials (America), Inc. | Filter-based extracellular vesicle nucleic acid isolation method |
| CN112662663B (zh) * | 2021-01-27 | 2022-11-08 | 苏州赛普生物科技有限公司 | 一种适于常温运输的rna提取试剂盒及提取方法 |
| EP4508209A1 (fr) * | 2022-04-15 | 2025-02-19 | Quantoom Biosciences S.A. | Procédés de séparation et/ou de purification d'acides nucléiques |
| EP4495250A1 (fr) * | 2023-07-18 | 2025-01-22 | Centogene GmbH | Procédé de préparation d'une préparation d'arn et son utilisation |
| WO2025078666A1 (fr) * | 2023-10-13 | 2025-04-17 | Quantoom Biosciences S.A. | Procédés de séparation et/ou de purification d'acides nucléiques |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE68920778T2 (de) | 1988-05-24 | 1995-05-18 | Anagen Uk Ltd | Magnetisch anziehbare Teilchen und Herstellungsverfahren. |
| DK1146049T3 (da) | 1994-02-11 | 2005-12-12 | Qiagen Gmbh | Fremgangsmåde til separering af dobbeltstrengede/enkeltstrengede nucleinsyrestrukturer |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| EP1260595B1 (fr) | 1995-07-07 | 2006-09-13 | Toyo Boseki Kabushiki Kaisha | Support magnétique pour acides nucléiques et procédé pour leur isolation en utilisant le dit support |
| KR100483498B1 (ko) | 1996-02-14 | 2005-07-18 | 악조 노벨 엔.브이. | 핵산물질의분리및증폭방법 |
| AU732726B2 (en) * | 1996-12-10 | 2001-04-26 | Purdue Research Foundation | Biomaterial derived from vertebrate liver tissue |
| ATE229371T1 (de) | 1997-01-21 | 2002-12-15 | Grace W R & Co | Siliciumdioxidadsorbent auf magnetischem träger |
| US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| ZA99493B (en) * | 1998-01-30 | 1999-07-22 | Akzo Nobel Nv | Method for the isolation of nucleic acid. |
| DE60137203D1 (de) | 2000-03-24 | 2009-02-12 | Qiagen Gmbh | Poröse ferro- oder ferrimagnetische glasteilchen für molekültrennung |
| DE10031236A1 (de) * | 2000-06-27 | 2002-01-10 | Qiagen Gmbh | Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien |
| GB0116359D0 (en) | 2001-07-04 | 2001-08-29 | Genovision As | Preparation of polymer particles |
| GB0215185D0 (en) | 2002-07-01 | 2002-08-07 | Genovision As | Binding a target substance |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| US20050059054A1 (en) | 2003-07-25 | 2005-03-17 | Richard Conrad | Methods and compositions for preparing RNA from a fixed sample |
| US20050026153A1 (en) * | 2003-07-31 | 2005-02-03 | Iannotti Claudia A. | Devices and methods for isolating RNA |
| EP2311994A1 (fr) * | 2003-08-01 | 2011-04-20 | Life Technologies Corporation | Compositions et procédés de préparation de courtes molécules d'ARN et d'autres acides nucléiques |
| CA2535153A1 (fr) * | 2003-08-12 | 2005-05-19 | Massachusetts Institute Of Technology | Procedes de preparation d'echantillons et dispositifs associes |
| CN1284852C (zh) * | 2004-09-09 | 2006-11-15 | 武汉大学 | 一种莲藕组织总rna的快速提取方法 |
| DE102004045332B3 (de) | 2004-09-16 | 2006-03-09 | Macherey, Nagel Gmbh & Co. Handelsgesellschaft | Verfahren zur Isolierung und Trennung von einzel- und doppelsträngigen Nucleinsäuren aus einer diese Nucleinsäuren enthaltenden Probe sowie Trennsäule zur Durchführung dieses Verfahrens |
| DE102007035250A1 (de) | 2007-07-27 | 2009-01-29 | Qiagen Gmbh | Verfahren zum Abtrennen von nicht-proteinhaltigen Biomolekülen, insbesondere Nukleinsäuren aus proteinhaltigen Proben |
| US7871764B1 (en) * | 2008-03-18 | 2011-01-18 | The United States Of America As Represented By The United States Department Of Energy | Universal nucleic acids sample preparation method for cells, spores and their mixture |
| CN101561415B (zh) * | 2008-04-18 | 2012-07-18 | 陈定虎 | 一种同时检测香蕉束顶病毒和花叶心腐病毒的检测方法 |
| CN101492485B (zh) * | 2009-03-13 | 2012-02-08 | 北京林业大学 | 一种提取裸子植物组织中rna的方法 |
| JP6204664B2 (ja) | 2010-02-26 | 2017-09-27 | キアゲン ゲーエムベーハー | Ｒｎａおよびｄｎａの並行単離および／または並行精製のためのプロセス |
| EP2407540A1 (fr) * | 2010-07-15 | 2012-01-18 | Qiagen GmbH | Procédé de purification d'un acide nucléique cible |
| WO2012028737A1 (fr) * | 2010-09-02 | 2012-03-08 | Qiagen Gmbh | Méthode d'isolation d'un acide nucléique cible comprenant des petits acides nucléiques cibles avec un rendement élevé |
| CN102344923A (zh) * | 2011-10-26 | 2012-02-08 | 浙江大学舟山海洋研究中心 | 海地瓜消化道总rna快速提取方法 |
-
2013
- 2013-09-03 JP JP2015529066A patent/JP6440616B2/ja active Active
- 2013-09-03 CN CN201380045814.0A patent/CN104603267B/zh active Active
- 2013-09-03 CA CA2880136A patent/CA2880136C/fr active Active
- 2013-09-03 AU AU2013310861A patent/AU2013310861B2/en active Active
- 2013-09-03 SG SG11201500655VA patent/SG11201500655VA/en unknown
- 2013-09-03 WO PCT/EP2013/068197 patent/WO2014033326A1/fr active Application Filing
- 2013-09-03 EP EP13759485.9A patent/EP2893015B1/fr active Active
- 2013-09-03 SG SG10201701747YA patent/SG10201701747YA/en unknown
- 2013-09-03 US US14/425,252 patent/US10329553B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015526100A (ja) | 2015-09-10 |
| US20150232831A1 (en) | 2015-08-20 |
| SG10201701747YA (en) | 2017-04-27 |
| WO2014033326A1 (fr) | 2014-03-06 |
| EP2893015B1 (fr) | 2018-05-02 |
| CN104603267B (zh) | 2019-08-27 |
| EP2893015A1 (fr) | 2015-07-15 |
| CN104603267A (zh) | 2015-05-06 |
| CA2880136C (fr) | 2023-08-15 |
| JP6440616B2 (ja) | 2018-12-19 |
| US10329553B2 (en) | 2019-06-25 |
| AU2013310861B2 (en) | 2019-02-14 |
| AU2013310861A1 (en) | 2015-02-12 |
| CA2880136A1 (fr) | 2014-03-06 |
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