RU2743001C1 - Feed probiotic additive for pigs - Google Patents

Abstract

FIELD: animal feed production.

SUBSTANCE: invention relates to feed production, in particular to a probiotic feed additive for pigs. The additive contains a bacterium concentrate, which is a mixture containing lactobacilli and yeast fungi, previously freeze-dried to the state of a lyophilized powder, dry milk whey, skimmed cow's milk powder, fulvic acid and water. All components are used in a specific ratio. In this case, the bacterial concentrate is formed by a mixture with Lactococcus lactis subsp. Lactis, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Vifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolescentis, Propionibacterium shermanii, Propionibacterium freudenrechii, Saccharomyces cerevisiae, Saccharomyces unisporus, Torulopsis sphaerica, Torulaspora delbrueskii, Candida kefir, Candida holmii, Candida friedrichii, Kluyveromyces lactis, Kluyveromyces marxianus.

EFFECT: use of the invention provides a high gain in live weight of animals.

1 cl, 3 tbl

Description

The invention relates to feed production, in particular to a probiotic feed additive for pigs [A23K 10/16, A23K 50/30].

Known feed additive used in the method of feeding broiler chickens and consisting of bacterial concentrate, which is a mixture of bacteria strains Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus lactis subsp. lactis and Propionobacterium freudenreichii subsp. shermanii, pre-freeze-dried to the state of lyophilized powder, lactulose, starch and perlite, at the following ratio: lyophilized bacterial concentrate powder - 10%, starch - 40%, lactulose - 30% and perlite - 20% (RU2604501 C1, publ. 10.12.2016, Bul. 34).

The disadvantage of the known feed additive is its limited scope, namely, the feed additive is used only for feeding broiler chickens.

Known feed additive with probiotic activity for farm animals, birds, horses and fish, containing in its composition the biomass of a complex of bacteria Enterococcus faecium 1-35 with a titer of live bacteria 3.8x10 -2.2x10 CFU and bacteria Bacillus megaterium with a titer of live bacteria 3, 8x10-3.3x10 CFU applied to a filler, which is used as bran or sunflower meal, or diatomite, or tripoli (RU2652836 C1, publ. 03.05.2018, bull. 13).

The disadvantage of the known feed additive is insufficiently high gain in live weight of fish at high feed costs per 1 g of live weight gain.

The closest to the claimed technical solution in terms of the composition of the bacteria included in the feed additive and the effect on the animal organism is the feed additive for farm animals "Lacto-plus", which includes a mixture of lactic acid bacteria Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus helveticus, Lactobacillus helveticus, fermentum, Lactobacillus casei, Lactococcus lactis, Streptococcus salvarius, Leuconostos citrovorum, Leuconostos dextranicum and a mixture of yeast fungi at a ratio of 3: 1 (RU2350101 C2, publ. 03/27/2009, bull. 9).

The prototype provides an improvement in digestion processes and better assimilation of feed by animals due to the fact that lactic acid bacteria contained in the inventive feed additive contribute to the suppression of pathogenic microflora, the production of hydrolytic enzymes that break down proteins, fats, carbohydrates, the decomposition of starch and cellulose with the formation of monosaccharides, and yeast fungi improve the secretion of the gastric and pancreas, enhance the absorption of nutrients in the intestines and increase the body's resistance to infections.

The content of a mixture of lactic acid bacteria and a mixture of yeast fungi in a ratio of 3: 1 allows for the most effective prevention of animals from diseases and stimulates processes in the digestive tract, in particular, cellulolytic processes.

The disadvantage of the known feed additive is its limited scope, namely, the feed additive is used only for feeding dairy cows and calves.

The technical problem of the prototype is the low average daily gain in live weight of fish, i.e. low productivity of animals with high feed costs per 1 g of live weight gain.

Also, the technical problem of the prototype is the limited shelf life of the feed additive. The additive in liquid form is stored for no more than 6 months.

The problem solved by this invention is to create a highly effective probiotic feed additive, the use of which in the diet of pigs will provide high productivity and low feed costs per 1 kg of live weight gain, and in addition, will increase the shelf life of the feed additive.

The technical result of the claimed invention is the creation of a probiotic feed additive for pigs, which, in comparison with the prototype, provides low feed costs per 1 kg of live weight gain and has a longer shelf life.

The technical result is achieved by the fact that the declared probiotic feed additive for pigs, consisting of a bacterial concentrate, which is a mixture of lactobacilli and yeast fungi, pre-freeze-dried to the state of lyophilized powder, characterized in that the feed additive additionally contains fillers: dry milk whey, dry fat-free cow's milk and fulvic acid, with the following ratio of components, wt%:

- lyophilized powder of bacterial concentrate - 0.4;

- dry milk whey - 6.0;

- skimmed cow's milk powder - 0.7;

- fulvic acid - 2.0;

- water - the rest,

where the bacterial concentrate is formed by a mixture in which the types of bacteria are present:

- Lactococcus lactis subsp. Lactis in the amount of 5x10 ⁸ CFU / g;

- taken in equal shares of Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum in a total amount of 1x10 ⁹ CFU / g;

- taken in equal shares of Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolescentis with a total amount of 2x10 ⁷ CFU / g;

- taken in equal proportions Propionibacterium shermanii, Propionibacterium freudenrechii - a total of 5x10 ⁸ CFU / g;

- a mixture of yeast fungi Saccharomyces cerevisiae, Saccharomyces unisporus, Torulopsis sphaerica, Torulaspora delbrueskii, Candida kefir, Candida holmii, Candida friedrichii, Kluyveromyces lactis, Kluyveromyces / marxianus, ⁹ g in total, taken in equal proportions.

Implementation of the invention

Theoretical background of the invention

In studies [Khamagaeva I.S. Biotechnology of starter cultures of propionic acid bacteria.-Ulan-Ude: Publishing house of VSGTU, 2006.− 172 p.] - [1]; [Vorobieva L.I. Propionic acid bacteria. - M .: Publishing house of Moscow State University, 1995. - 288 p.] - [2] of the classical propionic acid bacteria Propionibacterium freudenreichii and Propionibacterium shermanii it was shown that they form a trophic chain with lactic acid bacteria, pre-fermenting carbohydrates (including lactose) with the formation of lactic acid, which, in the form of lactates, propionic acid bacteria use as sources of carbon and energy.

According to [1, 2], the primary natural habitat of propionic bacteria, such as Propionibacterium freudenreichii and Propionibacterium shermanii, is the rumen of herbivores, where they ferment lactate formed by other representatives of the rumen microflora. In addition to lactate, these bacteria can ferment other sugars. Propionic acid bacteria are salt-tolerant. When the lactate medium contains 4% NaCl, normal growth and fermentation occurs.

Propionic acid bacteria synthesize significant amounts of fatty acids, lipids and phospholipids, the composition of which is a taxonomic feature. Some P. shermanii strains also synthesize C12, C21, C22, C23 fatty acids. The lipids of propionic acid bacteria, apparently, not only enter the structural elements of cells, but also play the role of protective components against the action of some antibiotics.

Propionic acid bacteria synthesize polyphosphates in significant quantities.

Propionic acid bacteria use lactate best in the presence of yeast extract. Lactate as a carbon source provides a higher growth rate of propionic acid bacteria than lactose.

These well-known research results [1, 2] showed that when 0.5% yeast extract is added to milk or whey, the production of acids by propionic acid bacteria increases 2.5-4.5 times, and when 1% sodium lactate is added, this the process is 1.6-4.6 times.

Also, studies [1, 2] show that the need for propionic acid bacteria in a set of vitamins is much lower than that of lactic acid bacteria. In studies [Delwiche E.A. Mechanim of propionic acid formation by Propionibacterium pentosaceum // J. Bact. - 1958. - Vol. 56. - P. 811-820.]; [Delwiche E.A., Carson S.F. A citris acid cycle in Propionibacterium pentosaceum // J. Bact. - 1968. - Vol. 65.- No. 3. - P. 318 –321] it is shown that they need only pantothenic acid and biotin, some of them require thiamine. Riboflavin stimulates growth, but there is no obligate need for it, bacteria synthesize vitamins B12 and B2 on their own. The growth of all propionic acid bacteria is stimulated by tween 80. Nisin has a strong inhibitory effect.

As a carbon source for synthesis, glucose is most favorable, but bacteria also grow well on lactose and lactate. Certain species curdle milk, do not liquefy gelatin, form diacetyl and acetoin.

Propionic bacteria can synthesize all amino acids by assimilating nitrogen (NH4) 2SO4. The biosynthesis of proteins by propionic bacteria is accompanied by the creation of a pool of 15 amino acids: cystine, histidine, arginine, aspartate, glutamic acid, glycine, serine, threonine, b-alanine, tyrosine, valine, methionine, proline, phenylalanine and leucine. It is known that bacteria contain peptidases, with the participation of which they provide themselves with essential amino acids and carry out transamination reactions, can grow on any of the 20 amino acids introduced into the medium as the only source of nitrogen.

According to [1, 2], the propionic bacteria Propionibacterium freudenreichii and Propionibacterium shermanii as sources of antibiomutagens or desmutagens can be used for pretreatment of food and feed in order to neutralize mutagenic (carcinogenic) substances, as well as probiotics. It was shown that the desmutagenic effect of the culture liquid was manifested when bacteria were grown on a medium with lactose and lactate.

Thus, propionic acid bacteria are among the most useful microorganisms. They are capable of synthesizing practically important substances: most amino acids, a significant amount of fatty acids, lipids and phospholipids, polyphosphates, enzymes and vitamins.

In [1, 2], it was shown that a distinctive feature of the metabolism of Propionibacterium freudenreichii and Propionibacterium shermanii is the close conjugation of energy processes and constructive metabolism. Propionic acid fermentation as the main energetic process effectively provides a constructive metabolism of classical propionic acid bacteria. At the same time, P. freudenreichii, in contrast to lactic acid bacteria, is able to synthesize all metabolites necessary to maintain their homeostasis in minimal media (containing cobalt ions) both anaerobically and in the presence of air. At the same time, it accumulates in cells or releases compounds into the environment, among which there are many useful for humans and animals, including nutraceuticals.

Also, according to [1, 2], propionic acid bacteria are used in baking. They, along with lactic acid bacteria and yeast, are introduced into some starter cultures for dough in order to form, in addition to lactic and acetic acids, propionic acids during fermentation.

All of the above indicated that when mixing yeast extract, the production of acids by propionic acid bacteria increases, and when lactose is added, this process is even more activated.

Moreover, these properties are characteristic of all strains of propionic acid bacteria, which is also indicated in [1, 2].

Therefore, in the present invention, this fact was taken into account when selecting the optimal ratios for a new feed additive, where the use of certain strains does not matter, since they all have the properties that are used in the invention.

In this application, the applicant uses properties generally known for all strains, expressed by a single general concept that does not have different private forms of implementation. Therefore, substantiating the validity of the degree of generalization of all strains used by the applicant, an extensive list of research results is indicated [1, 2], from which the possibility of those properties of bacterial species that are generally known for all strains of each species is confirmed.

Practical implementation of the invention

It was experimentally found that, unlike the prototype, it is advisable to use a mixture of bacteria of the following species:

- Lactococcus lactis subsp. Lactis in the amount of 5x10 ⁸ CFU / g;

- taken in equal shares of Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum in a total amount of 1x10 ⁹ CFU / g;

- taken in equal shares of Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolescentis with a total amount of 2x10 ⁷ CFU / g;

- taken in equal proportions Propionibacterium shermanii, Propionibacterium freudenrechii - a total of 5x10 ⁸ CFU / g;

- a mixture of yeast fungi Saccharomyces cerevisiae, Saccharomyces unisporus, Torulopsis sphaerica, Torulaspora delbrueskii, Candida kefir, Candida holmii, Candida friedrichii, Kluyveromyces lactis, Kluyveromyces / marxianus, ⁹ g in total, taken in equal proportions.

It was also taken into account that to feed propionic acid bacteria, it is necessary to add 0.7% skimmed cow's milk powder, 6% whey powder and 2% fulvic acid.

The concentration of living bacteria at the end of the shelf life is at least 2x10 ⁹ CFU / g.

The meaning of such selection of bacterial concentrate is that a certain type of bacteria is always present in the mixture in the required volume, which is determined by the total number of microbial cells at the end of the shelf life of at least 2x10 ⁹ CFU / g, divided by the number of all species used. In other words, the sufficiency of the presence of all types of the specified bacteria and fungi, taken in the specified amount, allows in any case to use their positive properties, which are generally known.

The claimed mixture of yeast fungi of the specified set is also well known and it is found in kefir and kurung, the positive properties of this set of yeast fungi are well known.

An innovative solution was the fact that in order to feed the specified aggregate mixture of bacteriological concentrate, it is necessary to add milk powder, skimmed milk powder, and fulvic acid.

It was the complex selection of bacterial concentrate as stated and the indicated ingredients of the nutritional mixture for it provided the following effect from the results of their interaction:

A) Mixing of lactic acid bacteria and yeasts provided the formation of the feed additive itself in full analogy with the prototype.

B) Mixing propionic acid bacteria with lactic acid bacteria ensured the start of fermentation with the formation of lactic acid, which, in the form of lactates, propionic acid bacteria were then used as sources of carbon and energy.

C) On the available energy sources (B), including skimmed cow's milk and dry milk whey, in the presence of yeast extract, propionic acid bacteria provided:

B.1) synthesis of a significant amount of fatty acids, lipids and phospholipids, polyphosphates;

B.2) synthesis of amino acids: cystine, histidine, arginine, aspartate, glutamic acid, glycine, serine, threonine, b-alanine, tyrosine, valine, methionine, proline, phenylalanine and leucine;

C.3) cleaning the feed mixture by neutralizing mutagenic (carcinogenic) substances.

D) The mixing of lactic acid bacteria and bifidobacteria in the cultured milk medium produced from milk and whey provided the expected and well-known effect characteristic of their interaction:

D.1) inhibition of the growth of potentially harmful microorganisms as a result of the production of antimicrobial substances; competition with them for adhesion receptors and nutrients; activation of immune-competent cells and stimulation of immunity;

D.2) stimulating the growth of representatives of the indigenous microflora as a result of the production of vitamins and other growth-stimulating factors; normalization of pH, eH-potential; neutralizing toxins;

D.3) restoration and optimization of the functioning of the biofilm lining the mucous membrane of the digestive tract;

D.4) a change in microbial metabolism leading to an increase or decrease in the synthesis and activity of bacterial enzymes and, as a consequence, the production of the corresponding metabolites (for example, VFA, glutamine, arginine, vitamins, peptidoglycans, etc.), which have the ability to locally or after penetration into the blood and other biological fluids of the macroorganism, directly interfere with the metabolic activity of the cells of the corresponding organs and tissues;

D.5) other mechanisms (direct effects of natural probiotics (lactic acid and bifidobacteria) after their absorption from the digestive tract on enzymatic and other cellular reactions of hormonal, nervous, excretory, immune and other organs and tissues).

The combination of the indicated complex effect (A, B, C, D) of the mixture selected experimentally made it possible to obtain a feed additive, which, unlike the prototype, was saturated with most amino acids, a significant amount of fatty acids, lipids and phospholipids, polyphosphates, enzymes and vitamins. At the same time, harmful microorganisms and carcinogens were neutralized in the feed additive, the additive had better digestibility.

Fulvic acid added to the feed additive not only contains a large amount of vitamins, amino acids, minerals, but is also an enzyme for catalysis of the fermentation reaction in a feed additive that improves bacterial cell division, as well as an antioxidant that neutralizes free radicals, increases resistance to viruses and bacteria, reduces acidity.

Fulvic acid allows you to protect the feed additive from the aggressive effects of the external environment, viruses, foreign bacteria, which increases its shelf life and reduces the requirements for storage conditions.

Fulvic acid reacts with minerals and breaks them down into ionic particles to form fulvates, the smallest possible forms of minerals. It is in this form that the minerals are easily absorbed by the body of animals, which has a positive effect on the growth of their mass. The low molecular weight ensures that fulvic acid is permeable through the cell membrane and is a conduit for vitamins and minerals in the body of animals.

This positive effect of fulvic acid on the increase in animal weight and increase the shelf life of the declared feed additive was revealed empirically.

The fulvic acid product is not cheap, therefore, more important in the selection of the required concentration in the fulvic acid mixture was the value of its minimum volume, which would give a positive effect, and beyond which the growth of a positive effect was no longer observed.

Experiments have shown that the feed additive was most effectively saturated with vitamins and had a long shelf life when 20 ml of fulvic acid was added to it per 1 kg of the feed additive. The feed additive in liquid form was stored in a refrigerator.

The experimental results are shown in Table 1, where the total number of microbial cells 100% is a value of 2x10 ⁹ CFU / g.

Table 1

Fraction of fulvic acid in ml per 100 mg of feed additive shelf life (100% - 1 year) 0.5 75 one 82 1.5 86 2 one hundred 2.5 98 3 97 3.5 97 four 96 4.5 97 five 98 5.5 97 6 96

From these data it can be seen that the effect of 20 ml of fulvic acid when added to the filler from whey dry and skimmed milk powder provides a longer shelf life of up to 1 year in the refrigerator compared to 6 months as in the prototype.

Lower values of fulvic acid (up to 20 ml per 1 kg) in the mixture sharply reduced the effectiveness of the feed additive on the increase in live weight, while higher values of fulvic acid (more than 20 ml per 1 kg) in the mixture did not affect the quality and positive properties of the feed. additives.

The experimental results are shown in Table 2.

Table 2 shows the experimental results of the selection of the ingredients of the mixture when changing the concentration of fulvic acid (per 1 kg of the mixture, taking into account 4 g of bacterial concentrate introduced).

table 2

For 1 kg of mixture Dry milk whey Skimmed milk powder The minimum efficiency of gain in live weight of pigs (%) Fraction of fulvic acid in ml per 100 mg of feed additive 0.5 6 0.7 5.4 2 6 0.7 8.7 3.5 6 0.7 8.3 five 6 0.7 8.5 6.5 6 0.7 7.9 8 6 0.7 7.8 9.5 6 0.7 7.4 eleven 6 0.7 6,7 12.5 6 0.7 6.2 14 6 0.7 5.7 15.5 6 0.7 4.9 17 6 0.7 4.2

The method of obtaining a feed additive is as follows.

A probiotic feed supplement can be prepared as follows.

In a 10 liter glass bottle, pour 1 liter of barley cake containing 1 × 10 ⁹ yeast cells, selected in equal shares of Saccharomyces cerevisiae, Saccharomyces unisporus, Torulopsis sphaerica, Torulaspora delbrueskii, Candida kefir, Candida fida holmirichi, Candida kefir, Candida fida holmirichi, marxianus and diluted with warm boiled water (18-20 ° C).

A ready-made mixture of bacteria of the following species is introduced into the resulting aqueous solution of barley skins:

- Lactococcus lactis subsp. Lactis in the amount of 5x10 ⁸ CFU / g;

- taken in equal shares of Lactobacillus delbrueckii subsp.bulgaricus, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum with a total amount of 1x10 ⁹ CFU / g;

- taken in equal shares of Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolescentis with a total amount of 2x10 ⁷ CFU / g;

- taken in equal shares Propionibacterium shermanii, Propionibacterium freudenrechii - a total of 5x10 ⁸ CFU / g,

which is dissolved in 100 ml of warm boiled water.

Next, 70 g of skimmed cow's milk powder, 600 g of dry milk whey and 200 ml of fulvic acid are added to the bottle.

The bottle is poured to the top with warm boiled water before reaching the 1-1.5 cm cork. The glass bottle is sealed with a cotton-gauze stopper and placed in a thermostat for 14 days, where the temperature is maintained within 34-35 ° C. Once within 2-3 days, the bottle is taken out of the thermostat, the lid is uncorked in order to allow air to enter and feed the aerobes, its contents are shaken to mix the grown microorganisms.

After 14 days, the bottle with the grown microorganisms is removed from the thermostat, its contents are filtered through paper filters, diluted with warm boiled water in a ratio of 1:10 and poured into a glass or plastic container.

The finished supplement is fed to animals.

Store the feed additive in liquid form - in the refrigerator for at least 1 year. The concentration of living bacteria at the end of the storage period must reach at least 2x10 ⁹ CFU / g.

Tests have shown that the obtained probiotic feed additive, with the optimal ratios of these components determined in in vivo experiments, provides an increase in their productivity and a decrease in feed costs per 1 kg of live weight gain when feeding pigs.

The effectiveness of the claimed probiotic feed additive is illustrated by examples, the initial indicators in which are its composition, and the experimentally determined indicators are the average daily gain in live weight of pigs in experimental groups for the period of scientific and production experience,% in relation to the control group and a decrease in feed costs per 1 kg of gain live weight of pigs in experimental groups,% in relation to the control group.

To test the effectiveness of the claimed probiotic feed additive when feeding pigs (fattening piglets), a research and production experiment was carried out in the conditions of ZAO Plemzavod Gulkevichsky, Gulkevichsky District, Krasnodar Territory.

To conduct a scientific and production experience, 5 groups of 50 heads in each group were formed from piglets for fattening of four months of age of the Landrace breed:

1st group - the control group, which received the main diet (without additives);

2nd group - experimental, receiving the main diet and additionally 0.3% to the mass of feed feed additive of known composition (prototype - additive "Lacto-plus");

3rd group - experimental, receiving the main diet and additionally 0.1% to the weight of the feed of the claimed probiotic feed additive according to the invention;

4th group - experimental, receiving the main diet and additionally 0.2% by weight of the feed of the claimed probiotic feed additive according to the invention;

5th group - experimental, receiving the main diet and additionally 0.3% by weight of the feed of the claimed probiotic feed additive according to the invention.

The conditions of keeping the piglets of all groups were the same and corresponded to the zootechnical standards. The duration of the research and production experience was 60 days.

Table 3 shows data characterizing the effectiveness of the claimed probiotic feed additive in comparison with the known feed additive.

Table 3

Indicator name Indicator value The famous
(2nd experimental group) Declared 3rd experimental group 4th experimental group 5th experimental group Average daily gain in live weight of animals in experimental groups over the period of the experiment,% to control 104.3 108.7 108.9 109.1 Reduction of feed costs per 1 kg of gain in live weight of animals in experimental groups during the experiment,% to control 2.4 6,3 6,3 6.4

From the data shown in Table 3, it can be seen that the average daily gain in live weight of animals in the experimental groups who received the claimed probiotic feed supplement in addition to the OR, compared with the control group receiving the OR (without additives), was 108.7-109.1%, and the average daily gain in live weight of animals that additionally received a known feed additive with RR, compared with the control group, is lower and amounted to 104.3%, that is, the effectiveness of using the claimed probiotic feed additive is 4.4-4.8% higher than that of the known one.

It should be noted that the use of the claimed probiotic feed additive in the diet of pigs provides a decrease in feed costs per 1 kg of live weight gain in comparison with the control group by 6.3-6.4%, and a known feed additive by 2.4%, i.e. e. the cost of feed with the use of the claimed probiotic feed additive is lower compared to the cost of feed with the use of a known feed additive by 3.9-4.0%.

Thus, the results of scientific and industrial experience confirm the higher efficiency of the claimed probiotic feed additive in comparison with the known feed additive.

Claims (8)

Probiotic feed additive for pigs, consisting of a bacterial concentrate, which is a mixture containing lactobacilli and yeast fungi, pre-freeze-dried to the state of a lyophilized powder, characterized in that the feed additive additionally contains fillers: milk powder, skimmed milk powder and fulvic acid, when the following ratio of components, wt%: lyophilized bacterial concentrate powder 0,4 dry milk whey 6.0 skimmed cow's milk powder 0.7 fulvic acid 2.0 water rest where the bacterial concentrate is formed by a mixture in which the types of bacteria are present: - Lactococcus lactis subsp. Lactis in the amount of 5x10 ⁸ CFU / g; - taken in equal shares of Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum in a total amount of 1x10 ⁹ CFU / g; - taken in equal shares of Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolescentis with a total amount of 2x10 ⁷ CFU / g; - taken in equal proportions Propionibacterium shermanii, Propionibacterium freudenrechii in a total amount of 5x10 ⁸ CFU / g; - taken in equal proportions a mixture of yeast fungi Saccharomyces cerevisiae, Saccharomyces unisporus, Torulopsis sphaerica, Torulaspora delbrueskii, Candida kefir, Candida holmii, Candida friedrichii, Kluyveromyces lactis, Kluyveromyces 1 g / ^{10 g / 10 g.}