RU2669299C1 - Biologically active animal feed supply for animals and birds and the method of its manufacture - Google Patents

Abstract

FIELD: cattle breeding.SUBSTANCE: invention relates to fodder biologically active additives. Biologically active feed additive for animals and birds contains the following components, wt. %: powder of diatomite fine-dispersed fraction 0–100 microns – 70–80, powder of aluminosilicate dry fine–grained fraction 0–100 microns – 5–15, bacteria Bacillus subtilis – 0.5–1 and Bacillus licheniformis – 0.5–1, yeast Saccharomyces cerevisiae – 5–15, powder of milk thistle – 0.5–1.5, citric acid – 0.5–1.5 and succinic acid – 0.5–1.5. Method for manufacturing a biologically active feed additive for animals and birds is that the diatomite is ground in a single-shaft clay-loosener with simultaneous drying at 450 °C to fine powder of fraction 0–100 microns. After that the diatomite fine powder and dry fine-porous aluminosilicate powder are mixed. Then bacteria and yeast are applied to the resulting mixture. Further, the citric and succinic acids are mixed with the milk powder and the mixture is sprayed onto a mixture of diatomite, aluminosilicate, bacteria and yeast. Then the entire formulation is mixed in an intensive mixer.EFFECT: invention allows to increase the growth of live weight, to reduce the death of the heads, to increase immunity and normalize digestion in animals and birds.2 cl, 6 tbl, 3 ex

Description

Technical field

The invention relates to feeds specifically intended for animals, namely to feed biologically active additives.

State of the art

Known RF patent for the invention No. 2528837 "ADDITION FROM VEGETABLE RAW MATERIALS AND METHOD FOR PRODUCING IT". Silicon-containing additive for food and feed purposes, as well as for fertilizers includes vegetable raw materials and a component selected from the group: soluble sugar, and / or baking soda, and / or catechin type chelating agent, in an amount of up to 40 wt. % subjected to mechanochemical activation. Moreover, more than 80% of the additive after mechanochemical activation at a temperature of no higher than 88 ° C have a particle size of less than 80 microns. As a plant raw material, the additive contains waste from the production of flour, the production of cereals, including rice husk, buckwheat husk, husk of oats, millet, and oatmeal. As a chelating substance of the catechin type - narrow-leaved Ivan tea, green tea, or a mixture thereof. The starting components are mixed before being fed to the activator mill, or the components are mixed simultaneously with mechanochemical activation in the activator mill. Mechanochemical activation is carried out at a temperature not exceeding 88 ° C for a time providing grinding of more than 80% of the additive to a particle size of less than 80 microns. Using the claimed invention will allow to obtain a silicon-containing additive for food and feed purposes, as well as for fertilizers with a minimum number of fractions and the prevention of inactivation and oxidation of the components of the mixture while increasing the solubility of silicon.

The disadvantage of this solution of the proposed supplement is that because of the high fiber content and stiffness, the digestive tract of animals is injured.

Closest to the claimed invention is the RF patent for the invention No. 2579219 "FODDER ADDITIVE FOR AGRICULTURAL ANIMALS AND BIRDS". The feed additive for farm animals and poultry contains bee mortality, biomass and culture fluid obtained from cultivation of the Kombucha Medusomyces Gisevii Lindau, bifidobacterin, pine and aspen bark extract, flask, bentonite and grass meal. All components are taken in a specific ratio. The use of the invention will stimulate metabolic processes, as well as remove heavy metals and mycotoxins from the body of animals.

The disadvantage of this solution is the presence of Kombucha, which, in case of an overdose of the feed additive proposed by the authors, can lead to upset stomach, as it causes an increase in the acidity of the stomach of animals, and an overdose of bifidobacteria can lead to metabolic disorders.

SUMMARY OF THE INVENTION The objective of the invention is to provide a biologically active feed additive for animals and birds, which helps to increase immunity, normalize digestion, increase weight and reduce mortality of animals and birds.

The technical result of the proposed composition for a biologically active feed additive for animals and birds is:

- increase in animal mass;

- reduction in the death of goals;

- increased immunity in animals and birds;

- normalization of digestion.

The problem is solved due to the fact that the biologically active feed additive for animals and birds contains silicon-containing material, bacteria, acids and a herbal supplement. As a silicon-containing material, a fine diatomite powder of a fraction of 0-100 microns and a dry fine-porous powder of an aluminosilicate fraction of a fraction of 0-100 microns are used. As bacteria: Bacillus subtilis, Bacillus licheniformis. In addition, the cell walls of the yeast Saccharomyces cerevisiae are present. And as a herbal supplement use milk thistle powder. The composition additionally contains citric and succinic acids. The composition is performed in the following ratio of components, mass%: diatomaceous powder 70-80%, aluminosilicate powder 5-15%, Bacillus subtilis 0.5-1%, Bacillus licheniformis 0.5-1%, Saccharomyces cerevisiae 5-15% - 10 , milk thistle powder 0.5-1.5%, citric acid 0.5-1.5%, succinic acid 0.5-1.5%.

A method of manufacturing a biologically active feed additive for animals and birds, is that diatomaceous earth is crushed in a single shaft shredder with simultaneous drying at 450 to a fine powder fraction 0-100 microns. After that, the fine-dispersed diatomite powder and dry fine-porous aluminosilicate powder are mixed. Then bacteria are applied to the resulting mixture. And then citric and succinic acid are mixed with milk thistle powder and yeast cell walls, and the resulting dry fine mass is introduced into a mixture of diatomite, aluminosilicate and bacteria. Then the whole composition is additionally mixed in an intensive mixer.

The implementation of the invention

The essence of the claimed invention is that a biologically active feed additive for animals and birds is a mixture of probiotic bacteria, cell walls of Saccharomyces cerevisiae yeast, milk thistle powder, citric and succinic acid and a silicon-containing filler containing water-soluble silicon.

Dietary supplement for animals and birds contains bacteria Bacillus subtilis, Bacillus licheniformis possessing proteolytic, pectinolytic, lipolytic and cellulolytic abilities, cell walls of the yeast Saccharomyces cerevisiae possessing a pronounced adsorbing ability to mycotoxin T-2, aflatoxins (G1, B2, G1, B2) , ochratoxin A, zearalenone, deoxynivalenol, as well as a set of vitamins necessary for the growth and development of the body, milk thistle powder containing a group of substances flavonolignans (silybi , Silibinin, silychristin, silidiadin) possess antioxidant, protective, anti-inflammatory, antiallergic, a reducing action of acids having antibacterial effect against pathogenic microorganisms and siliceous filler containing soluble silicon.

To prove the effectiveness of the proposed biologically active feed additives for animals and birds, the authors conducted studies for which they used the composition in the following ratios, mass%:

A silicon-containing material selected from diatomite (diatomaceous fine powder fraction 0-100 microns) is introduced into the composition as a base having a stable chemical composition and a high SiO ₂ content. Diatomite is used in many industries and agriculture. The use of diatomite to detoxify the body is perhaps its most popular use. When diatomite is consumed, microscopic “sponges” pass through the entire body of the animal, which absorb all contaminants, bacteria, viruses and fungi. That’s how diatomite works. When such porous “sponges” pass through the gastrointestinal tract, they are able to effectively absorb all residues of nutrients, toxins and other substances stored in the body. Diatomite contains negatively charged shells of diatoms that attract positively charged bacteria, fungi, mold, simple viruses, endotoxins, pesticides, drug residues and heavy metals. They bind in the cylindrical structure of the shell and are excreted in the feces.

In addition, diatomite particles are very hard and have sharp edges. Passing through the walls of the intestine, they delicately "scratch" its walls, sweeping the "debris" out of the body, without damaging the mucous membranes and walls of the intestine. But at the same time, thanks to the irregular shape, they kill with their protruding edges and larger and more dangerous parasites for animals.

Diatomite is one of the most recommended antibacterial and detoxification agents.

Diatomite is useful in balancing the lipid profile of the blood, significantly contributing to an increase in the level of “good” cholesterol and lowering the level of triglycerides. Silicon, contained in a huge amount in diatomite, is responsible for the elasticity of blood vessels, so it can be an element of the prevention of atherosclerosis. In addition, diatomite contains calcium and magnesium, which play a significant role in the normal functioning of the heart. Diatomite has high porosity, adsorption and acid resistance. An important property is the ability of a mineral to equalize the bioelectric potential of a living cell membrane, which helps to optimize its functional activity, increase resistance to external factors, restore its own drainage, transport, lipid properties of cell membranes. The use of diatomite during the accumulation of radioactive cesium in the body has a greater protective effect than the use of carbon-mineral sorbents.

Aluminosilicate has the appearance of a fine powder of a yellowish-white tint, odorless, insoluble in water, but decomposing upon interaction with hydrofluoric acid. Aluminosilicates are feldspars (albite, orthoclase, etc.), mica and clay type minerals. Aluminosilicates are widely used in the food industry, where their properties are most effectively manifested, aimed at preventing the rolling and clumping of food products and ingredients. This compound also has the properties of clarification and separation of bulk products. Aluminosilicate has a characteristic earthy flavor, has no impurities and can be combined with other types of silicates.

Application of aluminosilicate in the food industry aluminosilicate is labeled as food additive E 559 and is used in dry products, powders and mixtures. Aluminosilicate is used in the manufacture of dry dairy products (milk, cream, etc.), as well as granulated sugar, powdered sugar, edible salt, spices and seasonings. This silicate may be part of a salt substitute with properties similar to a natural product. This emulsifier is added to the composition of cheeses, as well as to cheese semi-finished products, which are wrapped in foil in sliced form. In accordance with technological instructions and sanitary and epidemiological standards, food supplement E 559 can be a part of dietary supplements, sugar tablets, confectionery products and canned foods. Aluminosilicates Synthetic aluminosilicate is used in winemaking, stock production, oil making, production of fruit juices and starch. In Europe, aluminosilicates are used for medical and cosmetic purposes. They are added to ointments and therapeutic gels, white clay, medications and creams. Aluminosilicate can be found in feed and vitamin supplements for animals and birds. As an aid, these silicates are used in the manufacture of packages, bags and paper products.

For the production of biologically active feed additives for animals and birds, pure cultures of Bacillus subtilis BS3 (B-11817 in the VKPM collection), Bacillus licheniformis (B-11170 in the VKPM collection) isolated from natural sources, certified and deposited in the prescribed manner, which were not exposed, are also used. gene modifications.

The bacteria Bacillus subtilis and Bacillus licheniformis are natural inducers of interferons, that is, they actively stimulate the formation of their own endogenous interferons in the body. This path is more natural than the introduction of artificial interferons in any way, and corresponds to the natural.

Propagating, bacteria with their proteases lyse all foreign proteins that are not characteristic of the body. At the same time, bacterial toxins and defective cells are destroyed, the body's immune status increases, and the regenerative processes of body tissues are stabilized.

Bacillus subtilis and Bacillus licheniformis possess the following main types of activity: antiviral effect; inhibits the growth and development of intracellular infectious agents of non-viral nature (chlamydia, rickettsia, bacteria, protozoa); antiproliferative activity; antitumor effect; antimutogenic effect; antitoxic effect; radioprotective effect; stimulates macrophages and enhances phagocytosis; enhances the cytotoxic effect of sensitized lymphocytes on target cells; enhances the formation of surface antigens; enhances or inhibits the activity of a number of cellular enzymes; enhances the cytotoxic effect of double-stranded RNA, inhibits or enhances antibody production, activates natural killer cells, stimulates the release of histamine by basophils, enhances the synthesis of prostaglandins, enhances the formation of histocompatibility antigens, and inhibits delayed-type hypersensitivity.

The bacteria Bacillus subtilis and Bacillus licheniformis are not normoflora elements in the microbial communities of humans and animals, but possess properties that provide the body with the ability to maintain microbocinosis at an ecologically natural level, optimize the metabolism and supply the body with biologically active and building materials, and ensure high-quality digestion of food. Under natural conditions, bacteria of these species always fell from the environment into the gastrointestinal tract (GIT), onto the skin and its damaged areas, the mucous membranes of humans and animals with dust, when eating plant food, and drinking water. Currently, more than 3,000 bacteria of these species are known. Industrial strains of bacteria Bacillus subtilis and Bacillus licheniformis are selected from the variety of bacteria of these species on the basis of maximum utility for humans and animals. In the process of producing bacteria in the technological cycle, these properties are controlled and maintained at a constant level. When bacteria enter the digestive tract, they live in it for no more than 30 days, after which they are excreted naturally.

The yeast of the species Saccharomyces cerevisiae (VKPM Y-3414) - produced by aerobic cultivation, is destroyed mechanically to obtain cell walls. The cell walls of the destroyed yeast cells are separated by centrifugation and washed, after which the suspension of the washed cell walls is subjected to ultrasound at a frequency of 18-30 kHz / s for 20-40 minutes. The optimal concentration of cell walls in suspension is from 1 to 5%. The power of ultrasonic exposure depends on the volume of the suspension and the concentration of cell walls in it.

As a result of ultrasonic treatment in the indicated mode, the inner layer of the cell wall formed by beta-glucan is physically separated from the outer layer of the cell wall formed by other polysaccharides. In fact, the yeast cell wall is stratified along its entire length and this is clearly visible by microscopic examination.

The composition of the cell wall includes protein-polysaccharide complexes and lipids. About 70% of the dry mass of the cell wall of the yeast is polysaccharides mannan and glucan. It is polysaccharides that play a large role in maintaining its mechanical strength.

For the production of biologically active feed additives for animals and birds are also used.

Milk thistle powder - The healing properties of milk thistle (milk thistle) have been known for many centuries. The active substances contained in it, up to 400, have a positive effect on human health. The forms of use of the product are very diverse. For example, milk thistle meal, the benefits and harms of which are disproportionate due to the predominance of positive properties, is an excellent source of fiber and reliable liver protection. Milk thistle - protects cell membranes and is a builder of young cells, stimulates the production of bile, neutralizes poisons. With the help of this substance, it is possible to prevent and even cure diseases of the gallbladder, liver. It has the ability to disinfect wounds, neutralize bacteria and microbes, strengthen the body's defense, lower cholesterol, remove toxic substances, regulate hormonal balance and oxygen metabolism. Milk thistle increases animal appetite, enhances metabolic processes, eliminates toxins, provides the body with vital enzymes, normalizes hormonal balance, transports hemoglobin, strengthens nervous energy, removes free radicals, normalizes cellular respiration, strengthens bone and connective tissue.

E330 citric acid is an antioxidant (otherwise - an antioxidant), has a natural or synthetic origin. Salts and esters of citric acid are called citrates. When heated above 175 ° C, it decomposes into carbon dioxide and water. The chemical formula is C ₆ H ₈ O ₇ . Citric acid is a tribasic carboxylic acid. It looks like small white crystals, the substance has good solubility in water and ethyl alcohol (calorizator). It exhibits weak properties of the acid itself. The taste is purely sour, not astringent. Participates in the metabolism of the body. Antioxidants, one of which is E330, are involved in the process of cell renewal, which slows down the aging process and has a positive effect on the condition of the skin, increasing their elasticity. Also E330 Citric acid is able to remove toxins and toxins through the pores of the skin, has a bactericidal effect.

Succinic acid Succinic acid (butanedioic or ethane dicarboxylic acid) is a product of the processing of natural amber, which is a completely harmless substance with a lot of useful properties. Succinic acid has the appearance of a white, crystalline powder and resembles the taste of citric acid. Succinic acid is found in any body, it is produced in the mitochondria (bodies several microns in size). Succinic acid is widely used in the field of medicine. The properties and effects of this substance are similar to the drug coenzyme Q10, which is widely used in Western countries as a means of prolonging youth, life and preventing the development of many diseases. The intake of succinic acid provides the cells with the necessary energy and helps to improve a number of biological processes. Regular intake of this substance can significantly slow down the aging process of the body, due to its ability to enhance cellular respiration, improve the absorption of oxygen by cells, and accelerate metabolism. In addition, it neutralizes free radicals and has a powerful antitoxic property. During the life of the body, aggressive forms of oxygen are formed, which oxidize and destroy cells, which leads to various diseases (cancer, heart attack), aging and death. Succinic acid in combination with glucose, as well as toxins, reduce the effects of intoxication, increase its ability to withstand the toxic effects of certain substances and free radicals.

Dietary supplement for animals and birds is used by adding it to regular animal and bird feeds.

The invention is illustrated by the following examples of the use of biologically active feed additives for animals and birds:

Example No. 1. (see table 1)

Experiments using biologically active feed additives for animals and birds were carried out on the basis of Bulanikhinskoye LLC. Dairy cows were fed the proposed dietary supplement for 300 days. As a result, their milk production increased by 12.8% compared with the control group. The highest milk yield after 300 days of lactation were in the cows of the 2nd experimental group, who received mineral supplementation as an additive, which was significantly (P≤0.01) higher than that of the analogues of the control group 10.6% (P≤0.05). The difference in milk yield between the cows of the control and experimental groups is statistically unreliable and amounts to 1.8%.

The smallest content of fat (3.75%) and protein (2.96%) was found in the milk of cows of the control group.

Thus, the inclusion in the rations of cows of a biologically active feed additive for animals and birds, all other things being equal, helps to increase their productivity, improve the quality of milk and, as a result, obtain a greater yield of fat and protein per lactation.

Example 2. (see table 2)

The studies were carried out on the basis of LLC Elkhovskaya Poultry Factory, Baryshsky District, Ulyanovsk Region.

During the production experiment, 2 groups of broiler chickens were formed (control and experimental 500 heads each). Chickens in the group were selected according to the method of analogues taking into account the cross, age, live weight, development. The conditions of detention, the front of feeding and drinking, the microclimate parameters in all groups were the same and corresponded to the recommendations of VNITIP.

From day 1 to day 7, the birds of both groups were fed starter compound feed. From day 8 to slaughter (day 42), the standard control feed was fed to the bird of the control group without adding additional feed additives, and the mixed test feed was fed to the test group bird, 1% of the proposed biologically active feed additive for animals and birds was introduced.

During the experiment, zootechnical indicators and economic efficiency were taken into account. Throughout the entire experimental period, the safety of the broiler chickens was taken into account, weighing was carried out, the average daily gain in live weight was determined, and feed consumption was taken into account (Table 2).

The use in the diets of broiler chickens of compound feed containing the proposed biologically active feed additive for animals and birds helped to increase the safety of the livestock, increase the weight of the carcass during slaughter, as well as reduce the consumption of feed in the experimental group.

Example 3. (see table 3)

Studies on the effect of biologically active feed additives for animals and birds on the productivity of fattening young pigs were carried out in LLC “Pig breeding Volzhsky” Cherdaklinsky district of Ulyanovsk region.

For the experiment, 50 pigs of large white breed at the age of 120 days were selected, divided into two experimental groups of 25 animals each and put on fattening. The dynamics of live weight of young pigs for fattening was traced up to 250 days.

The number of pigs in both experimental groups had a rather high growth energy

The pigs of the second experimental group had the best results in increasing live weight, the diets of which included the proposed biologically active feed supplement for animals and birds. At the age of 250 days, the maximum live weight was also observed in the second experimental group and amounted to 115.0 kg. They exceeded the pigs of the control group in live weight by 18.9 kg.

The advantages of the composition for biologically active feed additives for animals and birds:

- inhibition of the growth of potentially harmful microorganisms with antimicrobial products, activation of immunocompetent cells and, as a result, increased immunity in animals and birds;

- stimulation of growth as a result of active synthesis of growth-promoting factors, neutralization of toxins and restoration of symbiotic digestion;

- restoration of the functioning of the biofilm lining the mucosa of the digestive tract, and colonization resistance;

- a change in microbial metabolism, contributing to an increase or decrease in the synthesis and activity of bacterial enzymes and other components (VFA, amino acids, vitamins, etc.) that are capable of directly or locally interfering with the animal’s blood and other body fluids directly interfering with the metabolic activity of the corresponding cells organs and tissues;

- modulation of morphokinetic characteristics, physiological functions, biochemical and behavioral reactions of the animal;

- the direct action of the components after their absorption from the digestive tract, which has a positive effect on the enzymatic and other cellular reactions of hormonal, nervous, excretory, immune and other organs and tissues;

- increase the safety of young animals and increase daily gain.

All the above examples confirm the fulfillment of the task, namely, the creation of a biologically active feed additive for animals and birds, which helps to increase immunity, normalize digestion, increase weight and reduce mortality of animals and birds.

Claims (4)

1. A biologically active feed additive for animals and birds containing silicon-containing material, bacteria, yeast and a herbal supplement, characterized in that as a silicon-containing material, a powder of a finely dispersed diatomite fraction of 0-100 μm and a dry finely porous powder of an aluminosilicate fraction of 0-100 μm are used as bacteria: Bacillus subtilis, Bacillus licheniformis, Saccharomyces cerevisiae as yeast, and as a plant additive milk thistle powder, while the composition additionally contains citric and succinic acids, and the composition is performed in the following ratio of components, wt. %: Diatomaceous Powder 70-80 Aluminosilicate powder 5-15 Bacillus subtilis 0.5-1 Bacillus licheniformis 0.5-1 Saccharomyces cerevisiae 5-15 Milk thistle powder 0.5-1.5 Lemon acid 0.5-1.5 succinic acid 0.5-1.5 2. A method of manufacturing a biologically active feed additive for animals and birds according to claim 1, wherein the diatomaceous earth is crushed in a single shaft shredder with simultaneous drying at 450 ° C to a finely divided powder of a fraction of 0-100 μm, after which the diatomaceous powder is finely divided and dry finely porous aluminosilicate powder is mixed, and then bacteria and yeast are applied to the resulting mixture, then citric and succinic acids are mixed with milk thistle powder, and the resulting mixture is sprayed onto a mixture of diatomite, aluminosilicate b bacteria and yeast, then the whole composition is mixed in an intensive mixer.