RU2652835C1 - Method of productivity increasing and offspring preservation in pig breeding (options) - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture, in particular to fodder production. Method comprises daily administering a feed supplement in the form of a filler and a preparation with probiotic activity to the compound feed. As a preparation with probiotic activity two strains are used: a strain of Enterococcus faecium 1-35 bacteria and a strain of Bacillus megaterium B-4801 bacteria, which dried biomass in mixture in equal amounts is applied onto the filler in the form of calcined diatomite chips containing of 2.2×10⁸ CFU Enterococcus faecium 1-35 living bacteria and 2.1×10⁸ CFU of Bacillus megaterium B-4801 living bacteria per 1 g of feed additive. Feed additive is given once a day to the weaning and fattening group piglets during the entire piglets weaning and fattening period. Second variant of the method includes the daily administration of a feed additive in the form of a filler and a preparation with probiotic activity to the compound feed. As a preparation with probiotic activity two strains are used: a strain of Enterococcus faecium 1-35 bacteria and a strain of Bacillus megaterium B-4801 bacteria, which dried biomass in mixture in equal amounts is applied onto the filler in the form of tripolite containing of 1.3×10⁸ CFU Enterococcus faecium 1-35 living bacteria and 3.3×10⁸ CFU of Bacillus megaterium B-4801 living bacteria per 1 g of feed additive. Feed additive is given once a day to pregnant breeding pigs before farrowing for 10 days. Then continue to give to the milking sows for 30 days before the piglets weaning.

EFFECT: implementation of a group of inventions ensures an increase in the digestibility and digestibility of nutrients in rations and the improvement of the grown offspring zootechnical indicators.

2 cl, 6 tbl, 2 ex

Description

The invention relates to agriculture, in particular to the use of animal feed when a “Profort” dry feed additive is introduced into them with probiotic activity containing viable bacteria of the Enterococcus faecium 1-35 strain and viable bacteria of the Bacillus megaterium B-4801 strain for feeding pigs of weaning and fattening groups (Option 1) and for feeding sows (Option 2), while ensuring the safety and increased productivity of offspring.

A known method of growing young pigs, including the introduction of triticale as a grain component in full feed with an additional introduction of 0.1% by weight of feed, the complex enzyme preparation Natusim, including amyloglycosidase, hemicellulase, pentosanase, phosphatase, pectinase, and feeding his young animals throughout the growing period, RU No. 2580154 C1, A23K 50/30, A23K 50/75, A23K 10/12, 04/10/2016.

A known method of feeding young pigs, providing for the introduction into the main diet with a high content of barley enzyme preparations "Bio Feed Beta" and "Energex", which is administered together with the main diet in a ratio of 1: 1 in the amount of 260-320 mg / kg of the main diet, RU No. 2346459 C1, A23K 1/00, 02.20.2009.

A known method of feeding young pigs, including the introduction of feed additives in feed, consisting of 0.5 kg / t of dietary supplement "Prostor" and 100 mg / goal per day of flour from larvae of the fly Hermetia illucens, RU No. 2569628 C1, A23K 1/16 11/27/2015.

There is a method of increasing the productive qualities of young pigs, involving the use of Midium biologically active additives in animal feeding from 4.5 to 8.5 months of age daily at a dose of 10 g / kg of dry matter, RU No. 2271656 C2, A23K 1 / 00, A23K 1/10, 03.20.2006.

Known feed biologically active additive for young pigs containing waste culture products obtained after culturing cells, and additionally succinic acid, sodium nucleinate and organic selenium, RU No. 2483567 C1, A23K 1/00, A23K 1/16, A23K 1/18, 06/10/2013.

Known biologically active feed additive for piglets containing skim milk, bifidobacteria B. Bifidum, in the form of the drug "Bifidumbacterin" and dry leaf-stem supplement from stevia, RU No. 2367193 C2, A23K 1/00, A23K 1/14, A23K 1/16, 09/20/2009.

There is a known method of feeding piglets, including the introduction of dry beer grains and probiotic based on bacteria Bacillus subtilis into feed, while zeolite and dry corn gluten are added to feed with a ratio of components: 4.2% dry beer grains, 0.1% probiotic, 45 , 7% dry corn gluten and 50% zeolite, moreover, before introducing into the feed, the probiotic is mixed with zeolite, and then the resulting mixture is mixed with the rest of the components, after which the resulting mixture is introduced into the feed in the amount of 6% by weight of the feed and fed t piglets from 10 days of age, RU No. 2338387 C1, A23K 1/00, 11/20/2008.

Known synbiotic composition for the correction of dysbiotic disorders of the microbiocenosis of the gastrointestinal tract, including the prebiotic component and the probiotic component in the form of a combination of bacterial strains Lactobacillus delbrueckii TS1-06 and Lactobacillus fermentum TS3-06, as well as dried culture liquids with metabolites of probiotic bacteria strains Bacillus subtilis -2335 and Enterococcus faecium L-3, RU No. 2592988 C1, A61K 35/741, A61K 36/899, A61K 47/02, 07.27.2016.

Known feed additive with phytoprobiotic activity on a mineral basis, used in feed for farm animals and poultry and consisting of viable bacteria of the strain Enterococcus faecium 1-35 with a bacterium titer of at least 10 ⁶ CFU / mg, plant material and diatomite in the form of burnt chips, RU No. 2574689 C1, A23K 1/16, 02/10/2016.

A known bacterial strain Enterococcus faecium Ef790SAU with antagonistic activity against bacteria of the genus Listeria and species Enterococcus faecalis, deposited under registration number-1251 and used for the preparation of probiotic preparations, RU No. 2571852 C2, C12N 1/20, AK61/35/444, 20.12. 2015.

A known probiotic preparation intended to increase the gain in live weight of weaned piglets based on the B. subtilis strain TPI 13-07.06.21-DEP / VGNKI and the strain B. licheniformis TPI 11-07.06.22-DEP / VGNKI, which is a mixture of cultural liquids containing products of microbial synthesis of these strains, with a concentration of microbial cells of 5 billion m ³ / cm ³ , in a volume ratio of these strains 1: 1, which contains an equal mass mixture of bran and pea flour, taken in a ratio of 1: 1, RU No. 2399661 C1, C12N 1/20, A23K 1/16, 09/20/2010.

Known feed additive for young pigs containing dry beet pulp, probiotic, which includes bacterial cultures of Bifidobacterium globosum, Enterococcus faecium and Bacillus subtilis, fumaric acid and zeolite, RU No. 2359465 C1, A23K 1/00, 06/27/2009.

Known feed additive for piglets of the suckling period, including a culture of Lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton and a herbal supplement - dry powdery leaf-stem mass from stevia, RU No. 2348174 C1, A23K 1/16, 03/10/2009; RU No. 2342851 C1, A23K 1/00, A23K 1/14, A23K 1/16, 01/10/2009; RU No. 2342850 C1, A23K 1/00, A23K 1/14, A23K 1/16, 01/10/2009.

A known bacterial strain Bacillus megaterium var. phosphaticum BKM B-2357D, mobilizing phosphorus and silicon from lithosphere objects, which is promising for the production of a bacterial preparation, RU No. 232323737 C2, C12N 1/20, C05F 11/08, 06/27/2008.

It is known that a natural sorption mineral carrier, RU No. 2544629 C2, A23K 1/175, 03/20/2015; RU No. 2495589 C2, A23K 1/175, 10.20.2013; RU No. 2480025 C2, A23K 1/175, 04/27/2013; RU No. 2386343 C2, A23K 1/00, A23K 1/17, A23K 1/175, 04/20/2010; RU No. 2357424 C2, A23K 1/00, A23K 1/16, A23K 1/175, 06/10/2009; RU No. 2319391 C2, A23K 1/175, A23K 1/16, A23K 1/00, 03/20/2008.

In known methods of feeding pigs and in known feed additives, signs similar to the claimed invention were not found.

There is a method of increasing the productivity and safety of piglets after weaning, including the introduction into feed of a daily feed additive in the form of a filler and a preparation with probiotic activity, RU No. 2538379 C1, A23K 1/00, A23K 1/08, A23K 1/10, 10.01.2015.

This technical solution is made as the closest analogue of the present invention.

The closest analogue method involves introducing into feed compound daily feed additives in the form of zeolite in an amount of 7-8%, a probiotic from spore-forming bacteria of the Bacillus subtilis strain in an amount of 0.2-0.3% and glucose in an amount of 0.1-0.2% of the mass of the feed mixture, and the feed mixture from food waste is used as feed.

In the method of the closest analogue, the introduction into feed of feed additives of a different composition is not provided.

The present invention is based on the solution of the problem, which allows to increase the probiotic activity and quality of feed, increase the functionality of use and expand the application.

The technical result of the present invention is to provide a wide range of probiotic action of the feed supplement "Profort", providing the population of the gastrointestinal tract of animals with normal microflora, increasing digestibility and assimilation of nutrients of the diet by introducing live bacteria Enterococcus faecium 1-35 and live bacteria Bacillus megaterium B-4801, in increasing the sorption qualities of the “Profort” feed additive (Option 1), in improving the zootechnical parameters of further offspring rearing ( Option 2), in expanding the use of feeding the “Profort” feed additive to piglets of the weaning and feeding group (Option 1) and sows (Option 2).

According to the invention, this problem is solved due to the fact that the method of increasing the productivity and preservation of the offspring in pig breeding includes introducing a feed additive in the form of a filler and a preparation with probiotic activity into the feed daily.

Two strains are used as a preparation with probiotic activity: the bacterial strain Enterococcus faecium 1-35 and the bacterial strain Bacillus megaterium B-4801, the dried biomass of which in a mixture in equal amounts is applied to the filler.

Option 1

As a filler, burnt diatomaceous crumb containing live bacteria Enterococcus faecium 1-35 2.2 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 2.1 × 10 ⁸ CFU in 1 g of feed additive is used.

The feed additive is given once a day to the piglets of the weaning and fattening group during the entire period of weaning and fattening of the piglets.

Option 2

Tripoli containing live bacteria Enterococcus faecium 1-35 1.3 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 3.3 × 10 ⁸ CFU in 1 g of feed additive is used as a filler.

The feed additive is given once a day to pregnant sows before farrowing for 10 days, then continue to give suction sows for 30 days before weaning piglets.

The applicant has not identified sources containing information on technical solutions identical to this group of inventions, which allows us to conclude that their criterion of "novelty" is met.

Due to the implementation of the distinguishing features of the group of inventions (together with the features indicated in the restrictive part of the formula), important new properties of the object are achieved.

The use of the method for piglets of the weaning and feeding group (Option 1) and for pregnant sows, nursing sows (Option 2) expands the possibilities of the method.

Living bacteria Enterococcus faecium 1-35 contribute to the creation of normal microflora, increase digestibility and assimilation of nutrients in the diet.

Living bacteria Bacillus megaterium B-4801 when used together with living bacteria Enterococcus faecium 1-35 increase probiotic activity.

The use of carriers in the form of burnt diatomaceous crumbs (Option 1) and in the form of tripoli (Option 2) when interacting with a mixture of live bacteria Enterococcus faecium 1-35 and Bacillus megaterium B-4801 improves the growth and development of offspring.

The applicant is not aware of any publications that would contain information on the influence of the distinguishing features of the group of inventions on the achieved technical result. In this regard, according to the applicant, it can be concluded that the claimed technical solutions meet the criterion of "inventive step".

The present invention is carried out as follows.

Profort feed additive with probiotic activity is used as feed for farm animals and poultry, horses and fish.

The feed additive contains dried biomass of two strains: the bacterial strain Enterococcus faecium 1-35 and the bacterial strain Bacillus megaterium B-4801.

The bacterial strain Enterococcus faecium 1-35 was deposited in the collection of the All-Russian State Research Institute for the Control, Standardization and Certification of Veterinary Drugs (VGNKI) on March 20, 2008 under the registration number Enterococcus faecium VGNKI 08.03.53-DEP and is stored in the collection of microorganisms of BIOTROF LLC.

The bacterial strain Bacillus megaterium B-4801 was deposited in the All-Russian Collection of Industrial Microorganisms of FSUE GosNII Genetika under registration number VKPM: B-4801 and is stored in the collection of microorganisms of BIOTROF LLC.

Preparation of dry biomass of the bacterial strains Enterococcus faecium 1-35 and Bacillus megaterium B-4801 and the feed additive "Profort".

Experimental samples of cultures of bacteria strains Enterococcus faecium 1-35 and Bacillus megaterium B-4801 are produced in fermenters.

To obtain a feed supplement "Profort" take the dried biomass of the bacterial strain Enterococcus faecium 1-35 and the bacterial strain Bacillus megaterium B-4801.

The dried biomass of these strains is applied in a mixture in equal amounts to the filler.

Option 1

As a filler, burnt diatomaceous crumb containing live bacteria Enterococcus faecium 1-35 2.2 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 2.1 × 10 ⁸ CFU in 1 g of feed additive is used.

Diatomite - sedimentary rock loose or slightly cemented, consisting of the remains of diatoms and having a gray or yellow color of weak tones.

Chemically, diatomite is more than 80% composed of aqueous silica.

Diatomite in the form of burnt chips is supplied by the "Diatom Plant" in Inza, Ulyanovsk Region.

Option 2

Tripoli containing live bacteria Enterococcus faecium 1-35 1.3 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 3.3 × 10 ⁸ CFU in 1 g of feed additive is used as a filler.

"The safety of bacteria on different types of media" are shown in table 1.

The quantitative content of live bacteria is determined experimentally and is optimal.

A mixture of live bacteria Enterococcus faecium 1-35 and Bacillus megaterium B-4801 is applied to the fillers according to Options 1-2, respectively, and the wet components of the Profort feed additive are dried.

The drying of the components of the “Profort” feed additive is carried out in RT-ShS drying ovens by the air-heat method at a temperature from 55 ° C to 60 ° C for 9 hours to a final moisture content of 7.0-7.2%.

After drying, the components of the “Profort” feed additive are sent for grinding to the feed mill DKR-3.

Profort feed additive is ground to a powder state.

Studies on the implementation of the proposed method are presented in example 1 (Option 1) and example 2 (Option 2).

Example 1

The experience of using the “Profort” feed additive was carried out on the basis of JSC “Flame” in the Leningrad Region of the Gatchinsky District from March 2 to August 1, 2016 on pigs from weaning and feeding groups.

Two groups of analogues of piglets-weaners of 20 animals each and two groups of analogues of piglets of the fattening group of 15 animals each were formed by return and live weight. Group content - 10 goals in a cage of piglets of weaning groups and 15 goals in a cage of piglets of a feeding group.

Two-time feedings.

The main diet (RR) of weaned piglets (kg):

compound feed - 1.1; rolled grain - 0.5; silo - 0.6; milk powder - 35 g; serum - 3.0 l; fish oil - 0.2 g; Ministry of Internal Affairs - 300 g.

The main diet (RR) of young pigs of the fattening group:

May, June (kg): compound feed - 1.2; flattened grain, crushed - 1.8; silo - 2.5; serum - 8.0; grain waste - 0.5; meat broth - 0.1; Ministry of Internal Affairs - 0.150.

July (kg): compound feed - 1.5; silo - 2.5; serum - 8.0; grain waste - 0.5; meat broth - 0.1; Crusher - 1.5.

The first control group received the main diet (RR).

The second experimental group received the main ration (OR) with the Profort feed additive at the rate of 500 g / t of feed.

The results of the experiment are presented in table 2 "The influence of the feed additive" Profort "on the main zootechnical indicators of growing piglets of the weaning and fattening groups."

From table 2 it is seen that weaned piglets fed with the “Profort” feed additive showed better results compared to the control group: the average daily gain was 36% higher, the live weight of one head at the end of the experiment was 16.8% higher than the control group. At the same time, feed costs per 1 kg of gain of experimental piglets were lower than the control by 7.4%.

According to the results of the experiment, the dynamics of the weight gain when introducing the “Profort” feed additive into the feed is presented in table 3 “The effect of the“ Profort ”feed additive on the weight gain of piglets of the weaning and feeding group”.

From table 3 it is seen that the feed additive "Profort" has a positive effect on the growth and development of animals of the fattening group. The average daily gain in animals treated with the Profort feed supplement is 29.6% higher. The live weight of 1 head of the experimental group is 10.8% more than the control group and the cost of feed per 1 kg of weight gain, while reducing by 7.4%.

As a filler, we used burnt diatomaceous crumb containing 1 g of the Profort feed additive of live bacteria Enterococcus faecium 1-35 2.2 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 2.1 × 10 ⁸ CFU.

The introduction of the “Profort” feed additive in the diet of piglets of the weaning and fattening group contributed to increased appetite, improved digestibility of the feed and the digestibility of nutrients in the diet, which positively affected the growth and development of animals.

Example 2

The experience of using the “Profort” feed additive was carried out on the basis of JSC Flame PZ Leningrad Region of the Gatchinsky District from May 10 to July 18, 2016 on sows and suckling pigs.

Two groups of pregnant sows were formed, 10 animals in each group.

Two-time feedings.

The main ration (OR) of sows: silage - 2.5 kg, rolled grain - 2.0 kg; compound feed - 3.5 kg; serum - 8.0 l; Ministry of Internal Affairs - 0.2 kg.

The main diet (RR) of sucking pigs: serum - 1.5 l; fish oil - 0.6 g; compound feed - 0.2-0.5 kg; milk powder - 20 g; silo - training - 0.15 kg.

The first control group received the main diet (RR).

The second experimental group received the main ration (OR) with the Profort feed additive at the rate of 500 g / t of feed.

Feeding to pregnant sows of feed with the “Profort” feed additive was started 10 days before the farrowing, and then, for 30 days before weaning, pigs continued to feed the “Profort” feed additive in the main ration at the rate of 500 g per 1 t feed, after which the feed additive was introduced into the feed at the rate of 500 g of feed additive per 1 ton of feed for pigs of weaning and fattening groups.

According to the results of the experiment are presented in table 4 "The effect of the feed additive" Profort "on live weight and the safety of suckling pigs", in table 5 "The effect of the feed additive" Profort "on live weight and weight gain of piglets-suckers in the experimental group", in table 6 "The dynamics of live weight and weight gain of sucking piglets in the control group."

From table 4, 5, 6 it can be seen that the addition of the “Profort” feed additive to the diet during gestation 10 days before farrowing and after farrowing until weaning, increases the safety of the born piglets (control - 91.2%, experience - 96% ) average daily gains (control - 308.3 g, experience - 377.5); weight at weaning piglets of the experimental groups is higher than the control by 13.3%. An increase in the body’s immune status and general health when the “Profort” feed additive is added to the diet of sows and suckling pigs leads to a significant reduction in mortality, increased preservation and increased growth and development of piglets.

Tripoli was used as a filler, containing 1 g of Profort feed additive of live Enterococcus faecium bacteria 1-35 1.3 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 3.3 × 10 ⁸ CFU.

The results obtained on the basis of Examples 1-2 confirm the feasibility of introducing a feed additive "Profort" in the preparation of feed and feeding them when growing offspring in pig farming.

The proposed “Method for increasing the productivity and safety of offspring in pig breeding Variants” using the “Profort” dry feed additive with high probiotic activity obtained from known biotechnologies widely used in agriculture and research on the use of the “Profort” feed additive on the basis of the PZ “Flame” JSC of the Leningrad Region of the Gatchinsky District, according to the applicant, they determine compliance with the criterion “industrial applicability”.

The proposed "Method for increasing the productivity and safety of offspring in pig production Options" allows:

- increase the probiotic activity and quality of feed,

- expand the application of the method,

- implement the method with the feed additive "Profort",

- provide functional use capabilities.

Claims (2)

1. A method of increasing the productivity and safety of offspring in pig breeding, including introducing a feed additive in the form of a filler and a preparation with probiotic activity into the feed daily, characterized in that two strains are used as a preparation with probiotic activity: Enterococcus faecium 1-35 bacterial strain and strain bacteria Bacillus megaterium B-4801, the dried biomass of which in a mixture in equal amounts is applied to the filler in the form of a burnt crumb of diatomaceous earth containing in 1 g of a feed additive of live bacteria Enterococcus faecium 1-35 2.2 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 2.1 × 10 ⁸ CFU, and the feed supplement is given once a day to the piglets of the weaning and fattening group during the entire period of weaning and fattening of the piglets. 2. A method of increasing the productivity and safety of offspring in pig breeding, including introducing a feed additive in the form of a filler and a preparation with probiotic activity into feed daily, characterized in that two strains are used as a preparation with probiotic activity: Enterococcus faecium 1-35 bacterial strain and strain bacteria Bacillus megaterium B-4801, the dried biomass in the mixture in equal quantities is applied to the filler as a tripoli, containing 1 g of the feed additive viable bacteria Enterococcus faecium 1-35 1,3 × ¹⁰ August CFU of live and bact ry Bacillus megaterium B-4801 3,3 × 10 ⁸ cfu and feed additive given once a day pregnant sows before farrowing for 10 days, and then continue giving suckling sows during the 30 days prior to weaning.