RU2391816C1 - Method of enhancing colostral immunity in newborn calves with low viability - Google Patents

Abstract

FIELD: medicine. ^ SUBSTANCE: invention relates to the field of veterinary. Method includes single subcutaneous introduction of vaccine against paraflu-3 and infectious cattle rhinotracheitis dry cultural live in dose 2 ml to cows 1.5 month before delivery. Additionally cows are immunized with Coli-Vac K99 vaccine, intramuscularly, in dose 10 cm3 and 10-15 days after that in dose - 15 cm3. 15-60 minutes after birth 5% glucose solution in dose 1 ml/kg of body weight, 5% ascorbic acid solution in dose 2 ml per animal and intramuscularly 5% unitiole solution in dose 1 ml/10 kg of body weight are simultaneously introduced to newborn calves obtained from said cows. ^ EFFECT: method contributes to formation of more intense colostral immunity in newborn calves with low viability in comparison with analogues. ^ 12 tbl, 2 ex

Description

The invention relates to the field of veterinary medicine, relates to a method for increasing colostral immunity in newborn calves.

Colostral immunity is an immunity that forms in a newborn due to colostrum immunoglobulins during the first 24-36 hours of life. For newborns of some types of farm animals (ruminants, pigs and horses), antibodies are transmitted to offspring only through colostrum in the postnatal period [1]. Therefore, the intensity of absorption of adequate amounts of colostrum immunoglobulins is necessary for the acquisition of passive immunity. The insufficiency of its transfer determines the immunodeficiency state and the risk of diseases in newborns, mainly of infectious etiology [2].

Passive transport of colostral immunoglobulins is a transmembrane transfer by simple diffusion of immunoglobulins unchanged from the intestine into the blood during the first 24-36 hours of life. In the formation of colostral immunity in newborns, a major role is played by the acid-base state of their blood (CBS), which is largely determined by the characteristics of metabolic transformations during the prenatal period of development of animals and depends on the CBS of the mother’s blood [3]. Disorder of CBS (prolonged respiratory metabolic acidosis) occurs in newborn calves with reduced viability. The occurrence of an imbalance in the ratio of the intensity of lipid peroxidation processes and the functional activity of the antioxidant defense system during this period, being a significant factor in the disruption of passive transport of colostral immunoglobulins (reduces the level of colostral immunity), increases the risk of immunodeficiency and the occurrence of postnatal diseases in newborns.

There is a method of increasing the level of colostral immunity in newborn calves, which consists in the use of a complex mixture during the first and second drinking of colostrum in a dose of 250 ml. The mixture has the following composition (ml / l): allogenic immune serum of cattle - 450, extract from the spleen of cattle - 450, extract from the thymus of cattle - 90, saturated solution of succinic acid - 10 [4].

However, this method of forming colostral immunity is laborious, it suggests the use of autoclaves, Salnikov apparatus, laboratory glassware, etc. In addition, in the presence of acute or chronic infection in cattle, blood from such animals is not used for the production of allogeneic immune serum. Another disadvantage of this method is the low efficiency in the prevention of dyspepsia in newborn calves.

There is a method of formation of colostral immunity in young farm animals, which provides for a double administration of an inactivated vaccine to pregnant females, which is administered with an interval of 3 days 21 and 17 days before the expected delivery, respectively [5].

However, this method does not provide high preservation of calves in the first twenty days of life. Another disadvantage of this method is that in newborn calves with reduced viability, passive transport of colostral immunoglobulins is difficult due to prolonged respiratory metabolic acidosis.

A known method of increasing passive immunity in newborn animals, which consists in the use of immune sera [6]. The introduction of sera is accompanied by the formation in the animal after several hours of passive immunity, the duration of which is 10-14 days.

The main disadvantages of this method are multiple processing, laboriousness, the possible development of allergies, low therapeutic effect in viral infections.

A known method for the prevention of immunodeficiencies in newborns. After taking colostrum, immunomodulators are used [7].

However, this method of forming colostral immunity is laborious. Another disadvantage of this method is that to increase the level of colostral immunity requires several hours or days.

There is a method of increasing immunity in newborn calves, which consists in vaccinating pregnant cows with a vaccine against parainfluenza-3 and infectious rhinotracheitis of cattle, dry, culture-associated, live, which is administered subcutaneously at a dose of 2 ml [8]. Vaccination of pregnant animals leads to the formation of colostral immunity [9].

The disadvantage of this method is that the use of one vaccine does not allow to achieve a high level of immunity in cows and, as a result, high colostral (colostrum) immunity, which can provide adequate protection for calves from respiratory diseases.

The technical result of the invention is the formation of intense colostral immunity in newborn calves.

The technical result is achieved by immunization of deep-shelled cows with a parainfluenza-3 vaccine and cattle rhinotracheitis infectious dry culture associated, live and Kolya-Vak K99 vaccine, intravenous administration of 5% glucose solution to intravenous calves, intravenous administration of ascorbic acid and intramuscular administration of 5% - Unithiol solution.

Ascorbic acid plays an important role in the life of the body. Due to the presence of a diene group in the molecule, it has very pronounced reducing properties. Participates in the regulation of redox processes, carbohydrate metabolism, blood coagulation, in the regeneration of tissues, steroid hormones [10].

Unithiol, having active sulfhydryl groups, binds the so-called thiol poisons, forming non-toxic complexes that are excreted from the body by the kidneys. They combine not only with free poisons circulating in the blood, but also with those that have already bound to tissue proteins. Sulfhydryl groups interact with reactive oxygen species and peroxide radicals, restore the latter to non-toxic products, thereby reducing oxidative stress [10, 11, 12].

Glucose is one of the most easily digestible sugars. In the body, it breaks down with the formation of energy, which provides heat, the work of muscles and other tissues, reduces the level of lactic acid - normalizes the acid-base state of the blood. Glucose, introduced in an isotonic solution with 4.5%, replenishes the content of tissue fluid and delivers nutrients to the body [10].

The vaccine against parainfluenza-3 and infectious rhinotracheitis of cattle dry culture associated (VIEV). The vaccine is used in dysfunctional farms for the prevention of parainfluenza-3 and infectious rhinotracheitis in cattle. In reproductive farms, the vaccine is used in case of an outbreak. In this case, all healthy animals at risk of infection are forced to be vaccinated. Along with immunization, farms carry out a set of pet hygiene measures that increase the overall resistance of the animal organism. The vaccine is administered subcutaneously, in the middle third of the neck at a dose of 2 ml once. Immunity is formed 2 weeks after the start of vaccination; after re-vaccination, immunity lasts less than 6 months [8].

Vaccine against animal Escherichiosis Koli-Vak K88, K99, 987P, F41, TL and TS. The vaccine is intended for the prevention of Escherichiosis of farm animals and fur-bearing animals of cellular content. The vaccine is administered intramuscularly twice with an interval of 10-15 days, the first dose is 10 cm ³ , the second 15 cm ³ . The vaccine is used in farms that are unsuccessful for escherichiosis to vaccinate pregnant animals 1.5-2 months before birth, as well as piglets and lambs before weaning. Immunity is formed 7-10 days after the introduction of the second dose of the vaccine and persists in adult animals up to 6, in young animals up to 4 months [13].

The method is as follows.

1.5 months prior to calving, they are immunized with a vaccine against parainfluenza-3 and infectious rhinotracheitis of cattle with dry culture associated, live, subcutaneous, in the middle third of the neck at a dose of 2 ml once, at the same time they are immunized with the Kolya-Vak K99 vaccine, intramuscularly, at a dose of 10 cm ³ and after 10-15 days at a dose of 15 cm ³ . Newborn calves with reduced viability obtained from these cows, 15-60 minutes after birth, are simultaneously given intravenously 5% glucose solution at a dose of 1 ml / kg body weight and 5% ascorbic acid solution at a dose of 2 ml per animal and intramuscularly 5% solution unitiol in a dose of 1 ml / 10 kg of body weight.

The novelty of the method lies in the fact that for the first time a comprehensive method has been developed for increasing colostral immunity in newborn calves by the method of correction of metabolic status. The method is based on eliminating the imbalance in the intensity of lipid peroxidation processes and the functional activity of the antioxidant defense system during this period, which, together with long-term respiratory metabolic acidosis, are factors in violation of the passive transport of colostral immunoglobulins.

Testing the method of increasing colostral immunity was carried out in LLC Voronezhpishcheproduct of Novousmansky district of the Voronezh region in 2008 on 58 newborn calves. The experiments showed the effectiveness and feasibility of the proposed method. Data analysis in the scientific, technical and patent literature did not reveal a way to increase colostral immunity in calves according to the proposed scheme.

The essence of the method is illustrated by examples.

Example 1

The rationale for the application of the method

Depending on the viability of the calves, 2 groups of animals were formed. The first group included 10 calves with reduced viability, the second - 20 viable calves. The animals of the experimental groups were obtained from cows, which were immunized with a vaccine against parainfluenza-3 and infectious rhinotracheitis of cattle, dry, culture, live, subcutaneously, in the middle third of the neck at a dose of 2 ml once, 1.5 months before calving, once vaccinated at the same time. Koli-Wak K99, intramuscularly, at a dose of 10 cm ³ and after 10-15 days at a dose of 15 cm ³ . When determining viability, the time of realization of a confident standing posture, the appearance of a sucking reflex, body temperature, heart rate (HR / min), shortness of breath, the color of visible mucous membranes, and muscle tone were taken into account. Blood for biochemical and immunological studies was taken from 5-6 calves from each group on the 2nd and 14th day of life. In addition, feces were taken from 5 calves of 2-7 days of age with diarrheal syndrome, and from the fallen animals a small intestine with mesenteric lymph nodes and internal organs for bacteriological studies.

The animals were monitored for one month, the incidence of calves with omphalitis, gastrointestinal and respiratory diseases was taken into account. The research results are presented in tables 1-6.

Table 1 Ethological and physiological indicators of calves, depending on their viability Indicators Calf groups 1st (reduced viability) 2nd (viable) Temperature ° C on the 2nd day 38.2 ± 2.41 38.7 ± 0.17 on the 3rd day 38.8 ± 0.54 38.6 ± 0.27 Heart rate / min on the 2nd day 74.5 ± 22.3 44.3 ± 8.04 on the 3rd day 54.0 ± 16.5 37.0 ± 5.17 The appearance of a confident standing pose, min 83.0 ± 4.7 46.5 ± 7.96 The appearance of a sucking reflex, min 113.5 ± 3.1 64.8 ± 7.02

From table 1 it is seen that in calves of the first group, the appearance of a confident standing posture is realized at 83.0 ± 4.7 minutes after birth and the sucking reflex at 113.5 ± 3.1 minutes, the second much earlier - at 46.5 ± 7, 96 and 64.8 ± 7.02 minutes, respectively. In animals with reduced viability, body temperature on the 2nd day was 0.5 ° C lower, and the heart rate was higher by 38.9 (heart rate / min).

On the 3rd day of life, the body temperature in calves of the 1st group was 0.2 ° C higher in comparison with that of calves of the 2nd group. They also remained higher (by 11.4) heart rate.

table 2 The level of colostral immunity in newborn calves, depending on their viability Indicators Calf groups 1st (reduced viability) 2nd (viable) General IG, g / l on the 2nd day of life 12.8 ± 2.43 18.9 ± 0.79 on the 14th day of life 10.8 ± 2.02 16.4 ± 1.14 Escherichia antigen antibody titer on the 2nd day 1: 116 ± 46.4 1: 222 ± 39.2 on the 14th day 1: 223 ± 46.4 1: 150 ± 39.2 The titer of antibodies to antigens of the PG-3 virus on the 2nd day of life 1: 158 ± 2.5 1: 447 ± 3.9 on the 14th day of life 1: 158 ± 2.5 1: 294 ± 1.8 The titer of antibodies to antigens of the IRT virus on the 2nd day of life 1: 4.9 ± 2.30 1: 7.5 ± 0.50 on the 14th day of life 1: 13.0 ± 2.00 1: 2.5 ± 0.21

On the 2nd day, the level of total immunoglobulins and the titer of antibodies to the Escherichia antigen (Table 2) in calves of the 1st group was lower than in viable animals by 47.3% and 91.6%, respectively. Their titer of antibodies to antigens of PG-3 and RTI pathogens was 2.8 and 1.5 times lower, which indicates a lower humoral immunity against these respiratory infections.

Table 3 Incidence of calves with omphalitis depending on their viability Indicators Calf groups 1st (reduced viability) 2nd (viable) The number of calves, goal. (%) 10 (100) 20 (100) Of them sick, goal. (%) 6 (60) - It’s a goal. (%) - - The onset of the disease, days 1.66 ± 0.81 - Duration, days 4.1 ± 0.75 -

From table 3 it is seen that 60% of calves fell ill with omphalitis in the 1st group, and the disease was not recorded in the 2nd group.

Table 4 Incidence of calves with enteric form of Escherichiosis depending on their viability Indicators Calf groups 1st (reduced viability) 2nd (viable) The number of calves, goal. (%) 10 (100) 20 (100) Of them sick, goal. (%) 10 (100) 6 (30.0) It’s a goal. (%) 2 (20) - Start day 2.1 ± 0.73 2.7 ± 0.81 Duration, day 3.2 ± 0.63 2.8 ± 0.4

From table 4 it is seen that 100% of calves fell ill with the enteric form of escherichiosis in the first group. This is confirmed by a significant increase in the level of antibodies to Escherichia on the 14th day of life - 1: 223.3 ± 46.4 (table 2), which indicates the participation of this pathogen in the etiology of gastrointestinal diseases of calves. In addition, deaths of 2 (20%) animals were recorded in this group. Calves with reduced viability by 0.56 days fell ill earlier, and were sick 0.37 days longer than animals of the second group, among which 30% of enteric escherichiosis was observed, and there was no death.

The diagnosis of escherichiosis was confirmed by bacteriological studies (E. coli culture was isolated from feces of calves 2-7 days old with diarrhea, contents of the small intestine and mesenteric lymph nodes of dead calves. Serotypes 0149, 0138, 0139, 0142).

Table 5 The incidence of calves with tracheobronchitis depending on their viability Indicators Calf groups 1st (reduced viability) 2nd (viable) The number of calves, goal. %) 10 (100) 20 (100) Of them sick, goal. (%) 10 (100) 8 (40) It’s a goal. (%) - - Start day 11.0 ± 2.2 13.0 ± 2.7 Duration, day 9.9 ± 2.02 8.12 ± 1.12

From table 5 it is seen that the incidence of calves with tracheobronchitis in the 1st group was 2.5 times higher. They got sick 2 days earlier and were sick 1.8 days more.

Table 6 The metabolic status of newborn calves, depending on their viability Indicators Calf groups 1st (reduced viability) 2nd (viable) 2 days 14 days 2 days 14 days Total protein 70.1 ± 4.93 62.8 ± 1.65 76.1 ± 1.54 69.2 ± 1.32 Glucose 3.6 ± 1.06 3.5 ± 0.80 3.3 ± 0.38 3.8 ± 0.47 Lactate 2.1 ± 0.25 1.1 ± 0.20 1.3 ± 0.17 1.3 ± 0.15 pyruvate 171.3 ± 31.39 119.7 ± 34.90 158.9 ± 24.69 153.3 ± 18.23 Lactate / Pyruvate 12.3 9.2 8.2 8.4 Γ-HT activity 318.0 ± 58.4 51.7 ± 4.93 567.2 ± 93.9 109.4 ± 19.72 Creatinine 91.0 ± 6.56 75.0 ± 6.56 86.8 ± 5.18 71.6 ± 4.81 SOD 0.4 ± 0.05 0.5 ± 0.13 0.6 ± 0.10 0.7 ± 0.10 Catalase 38.6 ± 3.25 41.2 ± 1.92 41.1 ± 1.83 41.90 ± 1.00 GPO 31.1 ± 2.49 30.8 ± 1.38 27.9 ± 1.08 30.9 ± 1.23 CPU unit / ml 0.29 ± 0.07 0.31 ± 0.04 0.18 ± 0.009 0.33 ± 0.02 MDA 1.61 ± 0.07 1.67 ± 0.05 1.13 ± 0.10 1.15 ± 0.10 EI Index 28.7 ± 1.09 26.8 ± 1.19 22.4 ± 4.54 19.7 ± 1.50

From table 6 it is seen that in calves obtained from immunized cows against parainfluenza-3, infectious rhinotracheitis and bovine Escherichiosis, with reduced viability there was a more pronounced respiratory metabolic acidosis, as evidenced by a higher (1.5 times) value they have a lactate / pyruvate ratio compared to those of the second group. the active occurrence of free radical oxidation processes in calves of the first group was expressed by a high level of MDA and an endogenous intoxication index in their blood on both the 2nd and the 14th day of life. In the blood of these animals on the second day of postnatal development, the activity of the enzyme γ-glutamyltransferase, a marker of intestinal absorption of immunoglobulins, was 1.8 times lower than in viable calves.

Thus, the incidence of calves with omphalitis, tracheobronchitis and enteric form of Escherichiosis largely depended on the level of colostral immunity.

Example 2

Justification of effectiveness

Formed 2 groups of newborn calves with reduced viability obtained from cows that were vaccinated 1.5 days before calving with a vaccine against parainfluenza-3 and infectious rhinotracheitis of cattle with dry culture associated, lively, subcutaneous, in the middle third of the neck at a dose of 2 ml once, at the same time they were immunized with the Kolya-Vak K99 vaccine, intramuscularly, at a dose of 10 cm ³ and after 10-15 days at a dose of 15 cm ³ . The first group consisted of 12 calves, the second of 16.

Calves of the first group were injected intravenously with a 5% glucose solution at a dose of 1 ml / kg body weight to normalize respiratory metabolic acidosis, and the second group used a 5% glucose solution at a dose of 1 ml / kg body weight, 5%, to normalize respiratory acidosis and oxidative stress. a solution of ascorbic acid in a dose of 2 ml per animal and intramuscularly 5% solution of unitiol at a dose of 10 ml / kg body weight.

The drugs were administered 15-60 minutes after birth (depending on the clinical condition - reflex excitability, muscle tone, color of visible mucous membranes, heart rate, sucking reflex, etc.).

Assessment of the viability of calves, clinical observations of animals, blood sampling for biochemical studies was carried out, as in the first example.

The results of the experiment are presented in tables 7-12.

Table 7 Ethological and physiological indicators of calves, depending on the method of increasing colostral immunity Indicators Calf groups one 2 Temperature ° C on the 2nd day 38.6 ± 0.19 38.9 ± 0.30 on the 3rd day 38.9 ± 0.18 38.7 ± 0.36 Heart rate / min on the 2nd day 53.6 ± 8.6 44.4 ± 9.2 on the 3rd day 49.2 ± 8.4 44.0 ± 10.8 The appearance of a confident standing position, min 85.8 ± 7.8 75.8 ± 9.17 The appearance of a sucking reflex, min 104.0 ± 11.4 91.8 ± 12.2

From table 7 it is seen that the complex use of glucose with ascorbic acid and unitiol had a more favorable effect on the physiological status of animals. So, the appearance of a confident standing posture and sucking reflex in animals of the 2nd group were realized 10 and 12.2 minutes earlier, body temperature was 0.3 ° C higher by 2 days of life, and the heart rate was 5 times lower, respectively. 2 hours.s./min. On the 3rd day of life, the heart rate was also lower by 5.2 hours.s./min. The number of calves with shortness of breath in this group was also less.

Table 8 The level of colostral immunity in newborn calves, depending on the method of its correction Indicators Calf groups one 2 General IG, g / l on the 2nd day of life 14.5 ± 2.04 20.7 ± 1.00 on the 14th day of life 14.2 ± 2.22 16.9 ± 1.06 Escherichia antigen antibody titer on the 2nd day 1: 150 ± 58.1 1: 190 ± 68.9 on the 14th day 1: 116 ± 66.4 1: 158 ± 58.1 The titer of antibodies to antigens of the PG-3 virus on the 2nd day of life 1: 208 ± 9.8 1: 588 ± 6.2 on the 14th day of life 1: 239 ± 4.6 1: 417 ± 5.3 The titer of antibodies to antigens of the IRT virus on the 2nd day of life 1: 2.0 ± 0.67 1: 4.9 ± 1.33 on the 14th day of life 1: 2.5 ± 0.5 1: 4.0 ± 2.00

From table 8 it is seen that the combined use of 5% glucose solution, 5% ascorbic acid solution and 5% unitiol solution in calves contributed to an increase in their colostral immunity compared to animals that were injected with glucose only. The content of total immunoglobulins on day 2 was 6.26 g / l more, and antibody titers to the antigens of the PG-3 and IRT viruses were higher at 2.8 and 2.5, respectively. On day 14, these indicators of humoral immunity exceeded the comparison group by 2.7 g / l, respectively, 1.7 and 1.6 times.

Table 9 The incidence of calves with omphalitis, depending on the method of increasing colostral immunity Indicators Calf groups one 2 The number of calves, goal. (%) 12 (100) 16 (100) Of them sick, goal. (%) 4 (33.3) 4 (25) It’s a goal. (%) - - The onset of the disease, days 1.5 ± 0.5 2.0 ± 0.8 Duration, days 4,5 ± 0,5 3.75 ± 0.9

From table 9 it is seen that optimization of the physiological status of calves under the influence of drugs contributed to the formation of more pronounced colostral immunity, which ensured a 1.3-fold decrease in the incidence of calves with omphalitis.

Table 10 The incidence of calves with enteric form of escherichiosis, depending on the method of increasing colostral immunity Indicators Calf groups one 2 The number of calves, goal. (%) 12 (100) 16 (100) Of them sick, goal. (%) 8 (66.7) 6 (37.5) It’s a goal. (%) - - Start day 3.1 ± 1.6 3.0 ± 2.0 Duration, days 3.0 ± 0.5 3.7 ± 0.81

From table 10 it is seen that the combined use of a 5% glucose solution, a 5% ascorbic acid solution, and a 5% unitiol solution reduced the incidence of animals by an enteric form of escherichiosis by 1.8 times. The calves fell sick 0.12 days later and were sick 0.6 days less.

Table 11 The incidence of calves with tracheobronchitis, depending on the method of increasing colostral immunity Indicators Calf groups one 2 The number of calves, goal. (%) 12 (100) 16 (100) Of them sick, goal. (%) 12 (100) 14 (87.5) It’s a goal. (%) - - Start day 10.5 ± 3.5 12.8 ± 4.7 Duration, days 8.8 ± 1.3 8.33 ± 2.2

From table 11 it is seen that the combined use of 5% glucose solution, 5% ascorbic acid solution and 5% unitiol solution helped to reduce the incidence of tracheobronchitis by 12.5%. Calves became ill 2.3 days later and were sick 0.47 days less.

Table 12 The metabolic status of newborn calves, depending on the method of correction Indicators Calf groups one 2 2 days 14 days 2 days 14 days Total protein 70.9 ± 4.40 69.9 ± 3.91 76.6 ± 3.55 65.07 ± 2.93 Glucose 3.4 ± 0.31 3.5 ± 0.48 3.2 ± 0.57 4.01 ± 0.47 Lactate 2.0 ± 0.64 1.1 ± 0.22 1.3 ± 0.12 1.0 ± 0.09 Pyruvate 176.3 ± 33.85 120.6 ± 22.77 122.4 ± 24.80 142.0 ± 28.13 Lactate / Pyruvate 11.5 9.1 10.6 7.0 Γ-HT activity 493.8 ± 52.5 71.9 ± 25.33 875.3 ± 91.1 98.1 ± 24.62 Creatinine 102.6 ± 7.45 81.6 ± 9.4 104.0 ± 6.06 67.2 ± 5.42 SOD 0.6 ± 0.06 0.7 ± 0.13 0.6 ± 0.15 0.7 ± 0.09 Catalase 40.6 ± 1.58 43.15 ± 2.01 40.2 ± 0.82 38.63 ± 1.30 GPO 27.8 ± 1.01 31.5 ± 1.15 27.7 ± 1.30 30.3 ± 1.05 CPU unit / ml 0.16 ± 0.02 0.25 ± 0.04 0.19 ± 0.04 0.35 ± 0.04 MDA 1.26 ± 0.30 1.24 ± 0.19 1.06 ± 0.14 1.09 ± 0.11 EI Index 25.8 ± 1.63 21.3 ± 1.50 23.2 ± 3.22 20.8 ± 1.17

From table 12 it is seen that the use of 5% glucose solution, 5% ascorbic acid solution and 5% unitiol solution in newborn calves helped to reduce the phenomenon of acidosis. The ratio of lactate / pyruvate, which characterizes the ratio of the processes of aerobic and anaerobic oxidation of readily available energy sources, on calves of this group was 7.8% lower on the 2nd day than in animals that used a 5% glucose solution, with a decrease in the level of lactic acid by 35%, which corresponded to the state and orientation of oxidative processes in calves with a normal level of vitality.

The complex use of drugs was accompanied by a pronounced restraint of the lipid peroxidation process and the preservation of the functional activity of the AOD system. This was expressed in a lower level in the blood of MDA on both the 2nd and the 14th day of life compared with similar indicators in animals of the 1st group. In them, the phenomenon of endogenous intoxication on both the 2nd and the 14th day of life was less pronounced.

Thus, the correction of the metabolic status of newborn calves (stabilization of the processes of free-radical oxidation of the acid-base state) contributes to the creation of a higher level of colostral immunity, which is the basis for the prevention of postnatal diseases.

Information sources

1. Svendsen I. Lokal immunized og oral immunization mod mave - tarmsygdomme hos svinet forarsaget of E. coli // Dansk. Vet. Fidsskr. - 1978. - Agr. 61. - No. 4. - S.144-149.

2. Weaver D.M. Passive transfer of colostral immunoglobulins in calves / D.M. Weaver, J.W. Tyler, D.C. Van Meter et al. // J. Vet. Intern. Med. - 2000. - V.14, No. 6. - P.569-577.

3. Retskiy M.I. The role of the acid-base state in the formation of colostral immunity in newborn calves. / M.I.Retskii, A.G. Shakhov, A.I. Zolotarev, etc. // Bulletin of the Russian Agricultural Academy. - 2005. - No. 3. - S.69-71.

4. RU 2180573 C2, 03.20.2002.

5. RU 2305549 C1, 09/10/2007.

6. Efanova L.I. Protective mechanisms of the body, immunodiagnosis and immunoprophylaxis of infectious diseases of animals. / L.I. Efanova, E.T. Saidulin. - Voronezh: VGAU, - 2004. - P.188.

7. A scientifically based system for producing healthy young animals and for the prevention of gastrointestinal diseases of newborn calves. / Russian Academy of Agricultural Sciences // Sost. V.V.Subotin et al. - M .: 2002. - P.17.

8. Guidance on the use of vaccine against parainfluenza-3 and infectious rhinotracheitis of cattle dry culture associated (VIEV), live. // Approved by the Veterinary Department of the Ministry of Agriculture of Russia on 10.06.1996.

9. Khitrova A.E. New drugs for specific prophylaxis of mixed infectious diseases of calves. / A.E. Khitrova, G.L. Soboleva, T.I. Alipper // Veterinary life. - No. 1-2, 2005. - P.12.

10. Chervyakov D.K. Medicines in veterinary medicine / D.K. Chervyakov, P.D., Evdokimov, A.S. Vishker. - M .: Kolos, 1977 .-- 496 p.

11. Stadtman E.R. Metal ion-catalyzed oxidation of proteins: biochemical mechanism and biological consenquences. // Free Radic. Biol. and Med. - 1990. - V.9, No. 4. - P. 315-325.

12. Stadtman E.R., Oliver C.N. Metal catalyzed oxidation of proteins. // J. Biol. Chem. - 1991. - V.266. Number 4. - P.2005-2008.

13. Guidance on the use of a vaccine against escherichiosis in animals (Koli-Vak K88, K99, 987P, F41, TL and TS-toxoids). / Approved by the Veterinary Department of the Ministry of Agriculture of Russia on June 20, 1997.

Claims (1)

A method of increasing colostral immunity in newborn calves with reduced viability, including a single subcutaneous injection of cows 1.5 months before the birth of the vaccine against parainfluenza-3 and infectious rhinotracheitis of cattle with dry culture associated live dose of 2 ml, characterized in that it additionally cows at the same time they are immunized with the Kolya-Vak K99 vaccine, intramuscularly, at a dose of 10 cm ³ and after 10-15 days at a dose of 15 cm ³ , and to newborn calves received from these cows, 15-60 minutes after birth at the same time A 5% glucose solution at a dose of 1 ml / kg body weight, a 5% ascorbic acid solution at a dose of 2 ml per animal, and an intramuscularly 5% solution of unitiol at a dose of 1 ml / 10 kg body weight are administered intravenously.