[go: up one dir, main page]

RU2385928C3 - Продуцирование высокоманнозных белков в растительных культурах - Google Patents

Продуцирование высокоманнозных белков в растительных культурах

Info

Publication number
RU2385928C3
RU2385928C3 RU2005136874A RU2005136874A RU2385928C3 RU 2385928 C3 RU2385928 C3 RU 2385928C3 RU 2005136874 A RU2005136874 A RU 2005136874A RU 2005136874 A RU2005136874 A RU 2005136874A RU 2385928 C3 RU2385928 C3 RU 2385928C3
Authority
RU
Russia
Prior art keywords
mannous
proteins
production
plant crops
seq
Prior art date
Application number
RU2005136874A
Other languages
English (en)
Other versions
RU2385928C9 (ru
RU2005136874A (ru
RU2385928C2 (ru
Original Assignee
Проталикс Лтд.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Проталикс Лтд. filed Critical Проталикс Лтд.
Publication of RU2005136874A publication Critical patent/RU2005136874A/ru
Application granted granted Critical
Publication of RU2385928C2 publication Critical patent/RU2385928C2/ru
Publication of RU2385928C9 publication Critical patent/RU2385928C9/ru
Publication of RU2385928C3 publication Critical patent/RU2385928C3/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A61P31/22—Antivirals for DNA viruses for herpes viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01045—Glucosylceramidase (3.2.1.45), i.e. beta-glucocerebrosidase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Oncology (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Communicable Diseases (AREA)
  • Neurology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Claims (1)

  1. Лизосомальный белок человека, включающий глюкоцереброзидазу человека, которая включает аминокислотную последовательность, представленную в SEQ ID NO: 8, причем указанная глюкоцереброзидаза человека содержит по меньшей мере один ксилозный остаток и по меньшей мере один незащищенный маннозный остаток, и связана на своем С-конце с вакуолярным нацеливающим сигнальным пептидом, который представлен в SEQ ID NO: 2.
RU2005136874A 2003-04-27 2004-02-24 Продуцирование высокоманнозных белков в растительных культурах RU2385928C3 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IL155588 2003-04-27
IL15558803A IL155588A0 (en) 2003-04-27 2003-04-27 Methods for expression of enzymatically active recombinant lysosomal enzymes in transgenic plant root cells and vectors used thereby
PCT/IL2004/000181 WO2004096978A2 (en) 2003-04-27 2004-02-24 Production of high mannose proteins in plant culture

Related Child Applications (1)

Application Number Title Priority Date Filing Date
RU2009148012/10A Division RU2535342C2 (ru) 2003-04-27 2009-12-23 Продуцирование высокоманнозных белков в растительных культурах

Publications (4)

Publication Number Publication Date
RU2005136874A RU2005136874A (ru) 2006-06-10
RU2385928C2 RU2385928C2 (ru) 2010-04-10
RU2385928C9 RU2385928C9 (ru) 2021-07-19
RU2385928C3 true RU2385928C3 (ru) 2021-12-27

Family

ID=32697072

Family Applications (2)

Application Number Title Priority Date Filing Date
RU2005136874A RU2385928C3 (ru) 2003-04-27 2004-02-24 Продуцирование высокоманнозных белков в растительных культурах
RU2009148012/10A RU2535342C2 (ru) 2003-04-27 2009-12-23 Продуцирование высокоманнозных белков в растительных культурах

Family Applications After (1)

Application Number Title Priority Date Filing Date
RU2009148012/10A RU2535342C2 (ru) 2003-04-27 2009-12-23 Продуцирование высокоманнозных белков в растительных культурах

Country Status (20)

Country Link
US (1) US20060204487A1 (ru)
EP (6) EP2343378B1 (ru)
JP (2) JP4993693B2 (ru)
KR (3) KR20120093964A (ru)
CN (2) CN102240397A (ru)
AU (1) AU2004234635B2 (ru)
BR (1) BRPI0410520B8 (ru)
CA (1) CA2523539C (ru)
CY (1) CY1114893T1 (ru)
DK (1) DK2333083T3 (ru)
ES (2) ES2391057T3 (ru)
IL (3) IL155588A0 (ru)
MX (1) MXPA05011507A (ru)
PT (1) PT2333083E (ru)
RU (2) RU2385928C3 (ru)
SG (2) SG2007172737A (ru)
SI (1) SI2333083T1 (ru)
UA (1) UA89944C2 (ru)
WO (1) WO2004096978A2 (ru)
ZA (1) ZA200508680B (ru)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050032211A1 (en) * 1996-09-26 2005-02-10 Metabogal Ltd. Cell/tissue culturing device, system and method
US20030100945A1 (en) 2001-11-23 2003-05-29 Mindguard Ltd. Implantable intraluminal device and method of using same in treating aneurysms
US7951557B2 (en) * 2003-04-27 2011-05-31 Protalix Ltd. Human lysosomal proteins from plant cell culture
US20080274557A1 (en) * 2005-03-03 2008-11-06 Indiana University Research And Technology Permethylation Of Oligosaccharides
US20110070201A1 (en) * 2005-07-18 2011-03-24 Protalix Ltd. Mucosal or enteral administration of biologically active macromolecules
ATE543508T1 (de) 2006-10-31 2012-02-15 Yildiz Dr Med Yildiz Therapeutische verwendung von gba2
ES2660667T3 (es) 2007-05-07 2018-03-23 Protalix Ltd. Biorreactor desechable a gran escala
KR20100044813A (ko) * 2007-07-03 2010-04-30 이데미쓰 고산 가부시키가이샤 돼지 부종병 백신
EP2192924B1 (en) 2007-08-20 2017-10-11 Protalix Ltd. Saccharide-containing protein conjugates and uses thereof
ITUD20080055A1 (it) * 2008-03-13 2009-09-14 Transactiva S R L Procedimento per la produzione di una proteina umana in pianta, in particolare un enzima lisosomiale umano ricombinante in endosperma di cereali
JP5305284B2 (ja) * 2008-03-26 2013-10-02 独立行政法人産業技術総合研究所 糖化合物の質量分析法
EP2413962A1 (en) 2009-03-30 2012-02-08 Mount Sinai School of Medicine Influenza virus vaccines and uses thereof
EP2459212B1 (en) 2009-07-28 2018-03-14 Shire Human Genetic Therapies, Inc. Compositions and methods for treating gaucher disease
CN102596232A (zh) 2009-08-28 2012-07-18 西奈山医学院 用于治疗酸性鞘磷脂酶缺乏的剂量递增酶替代疗法
US9194011B2 (en) 2009-11-17 2015-11-24 Protalix Ltd. Stabilized alpha-galactosidase and uses thereof
WO2011061736A1 (en) 2009-11-17 2011-05-26 Protalix Ltd. Alkaline alpha galactosidase for the treatment of fabry disease
WO2011107991A1 (en) 2010-03-02 2011-09-09 Protalix Ltd. Glucocerebrosidase multimers and uses thereof
JP2013526849A (ja) 2010-03-30 2013-06-27 モウント シナイ スクール オフ メディシネ インフルエンザウイルスワクチン及びその使用
WO2012001641A1 (en) * 2010-06-30 2012-01-05 Actelion Pharmaceuticals Ltd Stable pharmaceutical compositions
WO2012050175A1 (ja) 2010-10-15 2012-04-19 日本ケミカルリサーチ株式会社 糖鎖の非還元末端がマンノース残基である糖蛋白質の製造方法
LT3272861T (lt) 2011-01-20 2020-03-25 Protalix Ltd. Alfa-galaktozidazės kompozicijos
KR101158673B1 (ko) * 2011-06-24 2012-07-03 주식회사 지씨바이오 재조합 인간 이듀로네이트-2-설파타아제를 포함하는 조성물, 제제 및 이의 제조방법
US20160145589A1 (en) 2011-06-24 2016-05-26 Green Cross Corporation Composition and formulation comprising recombinant human iduronate-2-sulfatase and preparation method thereof
HK1200102A1 (en) 2011-09-20 2015-07-31 西奈山医学院 Influenza virus vaccines and uses thereof
RU2014137404A (ru) 2012-02-19 2016-04-10 Проталикс Лтд. Пероральные единичные лекарственные формы и их применение для лечения болезни гоше
EP2863941B1 (en) 2012-03-02 2018-12-19 Shire Human Genetic Therapies, Inc. Velaglucerase for treating type iii gaucher disease
NZ627796A (en) 2012-12-18 2017-07-28 Icahn School Med Mount Sinai Influenza virus vaccines and uses thereof
JP6062074B2 (ja) * 2013-02-28 2017-01-18 ウンファ コーポレイション 組換え植物細胞、これの製造方法及びこれを利用した目的タンパク質の生産方法
CA2904035A1 (en) 2013-03-06 2014-09-12 Protalix Ltd. Tnf alpha inhibitor polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same
US9908930B2 (en) 2013-03-14 2018-03-06 Icahn School Of Medicine At Mount Sinai Antibodies against influenza virus hemagglutinin and uses thereof
EP2789686A1 (en) * 2013-04-11 2014-10-15 Greenovation Biotech GmbH Expression of phosphorylated glycoproteins in plants
WO2015118547A1 (en) 2014-02-10 2015-08-13 Protalix Ltd. Method of maintaining disease stability in a subject having gaucher's disease
EP3912635A1 (en) * 2014-07-11 2021-11-24 Biostrategies LC Materials and methods for treating disorders associated with sulfatase enzymes
US10000551B2 (en) 2014-09-11 2018-06-19 Protalix Ltd. Chimeric polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same
EA038931B9 (ru) * 2014-11-20 2022-02-18 Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд. Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений
WO2016118937A1 (en) 2015-01-23 2016-07-28 Icahn School Of Medicine At Mount Sinai Influenza virus vaccination regimens
ES2899783T3 (es) * 2015-03-17 2022-03-14 eleva GmbH Proteínas lisosómicas glucosiladas, método de producción y utilizaciones
CN109475795B (zh) * 2016-05-13 2021-06-01 安捷伦科技有限公司 用于自动对准、校准和标准化电泳数据的系统和方法
CN109641041A (zh) 2016-06-15 2019-04-16 西奈山伊坎医学院 流感病毒血细胞凝集素蛋白及其用途
EP3606555A4 (en) 2017-04-07 2021-08-04 Icahn School of Medicine at Mount Sinai INFLUENZA VIRUS TYPE B ANTI-NEURAMINIDASE ANTIBODIES AND THEIR USES
RU2668158C1 (ru) * 2017-06-28 2018-09-26 Общество с ограниченной ответственностью "Международный Биотехнологический Центр "Генериум" Способ очистки имиглюцеразы
CA3104297A1 (en) 2018-06-21 2019-12-26 Icahn School Of Medicine At Mount Sinai Mosaic influenza virus hemagglutinin polypeptides and uses thereof
WO2021213422A1 (en) * 2020-04-23 2021-10-28 Wuxi Biologics (Shanghai) Co., Ltd. A genetically modified cell line producing a recombinant glycoprotein having a mannose-terminated n-glycan
JP7583426B2 (ja) * 2020-05-21 2024-11-14 学校法人帝京大学 熱安定性グルコセレブロシダーゼ
WO2025019631A1 (en) 2023-07-19 2025-01-23 Icahn School Of Medicine At Mount Sinai Immunogenic compositions containing inactivated influenza a virus and cpg oligonucleotide adjuvant and uses thereof

Family Cites Families (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US467993A (en) * 1892-02-02 Alfred jorgensen
US2147271A (en) * 1937-07-16 1939-02-14 Schwarz Lab Inc Pure yeast culture apparatus
US2341259A (en) * 1941-01-22 1944-02-08 Lummus Co Inoculating method and apparatus
US2836434A (en) * 1952-08-21 1958-05-27 Separator Ab Arrangement for sterile interconnection and sealing of apparatuses in a plant for instance for cultivation of bacteria
US3201327A (en) * 1962-08-21 1965-08-17 Sun Oil Co Fermentation apparatus and process
SE371208B (ru) * 1966-10-06 1974-11-11 Sanraku Ocean Co
US3468520A (en) * 1967-05-12 1969-09-23 American Cyanamid Co Combination agitation and transfer unit for suspended cells
US3504185A (en) * 1968-05-09 1970-03-31 Univ Syracuse Res Corp Apparatus for measuring and controlling cell population density in a liquid medium
GB1284077A (en) * 1968-10-18 1972-08-02 British Petroleum Co Process and apparatus for the cultivation of micro-organisms
US3540700A (en) * 1969-01-24 1970-11-17 New Brunswick Scientific Co Rotary processing apparatus
DE2059931C3 (de) * 1970-12-05 1973-10-18 Knapsack Ag, 5033 Huerth-Knapsack Verfahren zum Verhindern von Schaumausbrüchen aus geschlossenen Reaktionsräumen
US3793154A (en) * 1970-12-21 1974-02-19 Univ St Johns Apparatus for gaseous environmental control of batch cultures of micro-organisms
US3950227A (en) * 1970-12-21 1976-04-13 St. John's University Batch method of establishing and maintaining a controlled aerobic environment for a microbial culture
US4179339A (en) * 1977-03-02 1979-12-18 Olympus Optical Company, Ltd. Liquid feeder for automatic culture apparatus
US4228243A (en) * 1978-07-13 1980-10-14 Toray Industries, Inc. Cell culture propagation apparatus
US4328317A (en) * 1980-03-31 1982-05-04 Solargizer International, Inc. Continuous chemical conversion or fermentation apparatus
DE3133314A1 (de) * 1981-08-22 1983-03-10 Hoechst Ag, 6000 Frankfurt "vorrichtung zum dispergieren einer zweiten phase in einer ersten phase"
US4519984A (en) * 1982-01-18 1985-05-28 Phillips Petroleum Company Apparatus for carrying out sparged reaction
CH663422A5 (de) * 1982-05-27 1987-12-15 Chemap Ag Verfahren und fermenter zur zuechtung von gewebezellen.
US4708938A (en) * 1984-04-30 1987-11-24 Hickinbotham Winemakers Pty. Ltd. Alcoholic fermentation
US4668632A (en) * 1985-02-06 1987-05-26 Vxr, Inc. Sparger and apparatus for and method of growing cells
US5372945A (en) * 1985-06-06 1994-12-13 Alchas; Paul G. Device and method for collecting and processing fat tissue and procuring microvessel endothelial cells to produce endothelial cell product
CN85100412B (zh) * 1985-06-25 1988-02-10 山东省食品发酵工业研究所 用嗜热放线菌生产固定化葡萄糖异构酶的方法及其用于高果糖糖浆的生产方法
US4713345A (en) * 1985-07-29 1987-12-15 Grain Processing Corporation Fermentation methods and apparatus
EP0224962A1 (en) * 1985-11-25 1987-06-10 De Staat Der Nederlanden Vertegenwoordigd Door De Minister Van Welzijn, Volksgezondheid En Cultuur Apparatus for the continuous cultivation of microorganisms in a culture liquid
US5166072A (en) * 1986-06-26 1992-11-24 Bayer Aktiengesellschaft Apparatus for the cultivation of immobilized micro-organisms
US4717668A (en) * 1986-12-12 1988-01-05 Baxter Travenol Laboratories, Inc. Plastic roller bottle for suspension cultures
US5081036A (en) * 1987-01-23 1992-01-14 Hoffmann-La Roche Inc. Method and apparatus for cell culture
US4908315A (en) * 1987-03-04 1990-03-13 Agristar, Inc. Integument and method for micropropagation and tissue culturing
US4894344A (en) * 1987-11-28 1990-01-16 Nippon Shinyaku Co., Ltd. Method for manufacturing 2-amino-2-deoxy-D-mannitol
US6846968B1 (en) * 1988-02-26 2005-01-25 Large Scale Biology Corporation Production of lysosomal enzymes in plants by transient expression
US4931401A (en) * 1988-09-01 1990-06-05 La Societe De Recherche Snc Inc. Bioreactor
US5549892A (en) 1988-12-23 1996-08-27 Genzyme Corporation Enhanced in vivo uptake of glucocerebrosidase
US5073491A (en) * 1988-12-23 1991-12-17 Hoffman-La Roche Inc. Immobilization of cells in alginate beads containing cavities for growth of cells in airlift bioreactors
US5100801A (en) * 1989-01-26 1992-03-31 Biocontrol Systems, Inc. Device for sequential microbial enrichment in a single apparatus
US5188946A (en) * 1989-01-26 1993-02-23 Biocontrol Systems Incorporated Process and device for sequential microbial enrichment in a single apparatus
JP2514102B2 (ja) * 1990-06-13 1996-07-10 ユリカ株式会社 ユリ科アロエ属に属する植物の真空凍結乾燥体を利用した錠剤製品及びその製造方法
DE59109267D1 (de) * 1990-06-15 2005-04-07 Syngenta Participations Ag Neue Signalsequenzen
EP0471947A1 (en) * 1990-06-29 1992-02-26 Sekisui Chemical Co., Ltd. Culture bag
US5240598A (en) * 1990-09-18 1993-08-31 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Microbubble generator for the transfer of oxygen to microbial inocula and microbubble generator immobilized cell reactor
US5401650A (en) * 1990-10-24 1995-03-28 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of biologically active α-galactosidase A
EP0485970A3 (en) 1990-11-13 1992-07-01 Yeda Research And Development Company Limited Transgenic plants overproducing threonine and lysine
AT394576B (de) * 1991-01-16 1992-05-11 Vogelbusch Gmbh Reaktor zur durchfuehrung biologischer reaktionen mittels biokatalysatoren
US5612188A (en) * 1991-11-25 1997-03-18 Cornell Research Foundation, Inc. Automated, multicompartmental cell culture system
US5267791A (en) * 1991-12-13 1993-12-07 Corning Incorporated Suspended cell culture stirring vessel closure and apparatus
JPH05219834A (ja) * 1992-02-14 1993-08-31 Fumiko Kobayashi ディスポーサブル培養袋及び培養方法
IL102189A (en) * 1992-06-12 1995-07-31 Univ Ben Gurion Device for growing microorganisms
US5409833A (en) * 1993-07-01 1995-04-25 Baxter International Inc. Microvessel cell isolation apparatus
US5342781A (en) * 1993-07-15 1994-08-30 Su Wei Wen W External-loop perfusion air-lift bioreactor
WO1996012801A1 (en) * 1994-10-24 1996-05-02 The Texas A & M University System Oral immunization with transgenic plants
ES2326705T3 (es) * 1995-09-14 2009-10-16 Virginia Tech Intellectual Properties, Inc. Produccion de enzimas lisosomicas en sistemas de expresion basados en plantas.
EP0879293A1 (de) * 1996-02-08 1998-11-25 Institut Für Pflanzengenetik Und Kulturpflanzenforschung Kassetten zur expression von lagerstabilen proteinen in pflanzen
US6458574B1 (en) * 1996-09-12 2002-10-01 Transkaryotic Therapies, Inc. Treatment of a α-galactosidase a deficiency
US6083725A (en) * 1996-09-13 2000-07-04 Transkaryotic Therapies, Inc. Tranfected human cells expressing human α-galactosidase A protein
US20050032211A1 (en) * 1996-09-26 2005-02-10 Metabogal Ltd. Cell/tissue culturing device, system and method
IL119310A (en) * 1996-09-26 1999-07-14 Metabogal Ltd Cell/tissue culturing device and method
US6190913B1 (en) * 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
US6096546A (en) * 1998-01-30 2000-08-01 Board Of Trustees, Rutgers, The State University Of New Jersey Methods for recovering polypeptides from plants and portions thereof
AU6021699A (en) * 1998-09-01 2000-03-21 Penn State Research Foundation Method and apparatus for aseptic growth or processing of biomass
CA2344269A1 (en) * 1998-10-07 2000-04-13 Syngenta Participations Ag Therapeutically active proteins in plants
US6432698B1 (en) * 1999-01-06 2002-08-13 Rutgers, The State University Disposable bioreactor for culturing microorganisms and cells
JP2002000248A (ja) * 2000-06-23 2002-01-08 Daiichi Kagaku Kk 凍結法及び凍結乾燥法
DE60142971D1 (de) * 2000-07-31 2010-10-14 Biolex Therapeutics Inc Expression biologisch aktiver polypeptide in wasserlinse
US7138262B1 (en) * 2000-08-18 2006-11-21 Shire Human Genetic Therapies, Inc. High mannose proteins and methods of making high mannose proteins
AU2002232803A1 (en) * 2000-11-15 2002-05-27 Genzyme Corporation Expression system for recombinant proteins
WO2002081695A2 (en) * 2001-04-06 2002-10-17 Syngenta Participations Ag Genes that are modulated by posttranscriptional gene silencing
DE60233395D1 (de) * 2001-04-18 2009-10-01 Valtion Teknillinen Die verwendung von abc transporter kodierenden genen zur stimulierung der herstellung von sekundärmetaboliten in biologischen zellen
US7629309B2 (en) * 2002-05-29 2009-12-08 Zystor Therapeutics, Inc. Targeted therapeutic proteins
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
US7951557B2 (en) * 2003-04-27 2011-05-31 Protalix Ltd. Human lysosomal proteins from plant cell culture
CA2545160A1 (en) * 2003-11-06 2005-05-19 Compugen Ltd. Variants of human glycoprotein hormone alpha chain: compositions and uses thereof
DE602005007423D1 (de) * 2004-06-02 2008-07-24 Millipore Corp Einwegreaktor
CN102492607B (zh) * 2004-06-04 2014-12-10 通用电气医疗集团生物科学公司 一次性生物反应器系统及方法
JP2008515454A (ja) * 2004-10-13 2008-05-15 プロタリクス リミテッド 植物細胞培養における抗体製造のシステムおよび方法
US20080132743A1 (en) * 2006-12-01 2008-06-05 Albemarle Corporation Production of high purity decabromodiphenylalkanes
ES2660667T3 (es) * 2007-05-07 2018-03-23 Protalix Ltd. Biorreactor desechable a gran escala

Also Published As

Publication number Publication date
BRPI0410520B1 (pt) 2021-02-23
AU2004234635A1 (en) 2004-11-11
JP2011184445A (ja) 2011-09-22
BRPI0410520B8 (pt) 2021-05-25
JP4993693B2 (ja) 2012-08-08
WO2004096978A2 (en) 2004-11-11
CN102240397A (zh) 2011-11-16
SG2007172737A (en) 2014-09-26
ES2391057T3 (es) 2012-11-21
IL155588A0 (en) 2003-11-23
EP2343378A1 (en) 2011-07-13
RU2385928C9 (ru) 2021-07-19
ZA200508680B (en) 2008-10-29
KR101332372B1 (ko) 2013-11-27
EP2330204A1 (en) 2011-06-08
CA2523539A1 (en) 2004-11-11
KR20120093964A (ko) 2012-08-23
IL182888A0 (en) 2007-08-19
UA89944C2 (ru) 2010-03-25
BRPI0410520A (pt) 2006-06-20
ES2446965T3 (es) 2014-03-11
KR20110089193A (ko) 2011-08-04
US20060204487A1 (en) 2006-09-14
CN1875111B (zh) 2011-06-29
RU2005136874A (ru) 2006-06-10
CN1875111A (zh) 2006-12-06
EP1618177B1 (en) 2012-06-06
EP2322636A1 (en) 2011-05-18
HK1088041A1 (en) 2006-10-27
AU2004234635B2 (en) 2010-05-13
JP2006524506A (ja) 2006-11-02
RU2009148012A (ru) 2011-06-27
PT2333083E (pt) 2014-02-26
MXPA05011507A (es) 2006-07-03
EP1618177A4 (en) 2007-07-04
KR20060018826A (ko) 2006-03-02
EP1618177A2 (en) 2006-01-25
EP2333083B1 (en) 2014-01-01
EP2330205B1 (en) 2015-01-21
SG2013021217A (en) 2015-08-28
CY1114893T1 (el) 2016-12-14
CA2523539C (en) 2017-06-06
EP2322636B1 (en) 2015-04-08
IL171561A (en) 2014-09-30
RU2535342C2 (ru) 2014-12-10
EP2343378B1 (en) 2014-09-10
IL182888A (en) 2014-08-31
SI2333083T1 (sl) 2014-03-31
DK2333083T3 (da) 2014-01-27
RU2385928C2 (ru) 2010-04-10
WO2004096978A3 (en) 2006-03-30
EP2333083A1 (en) 2011-06-15
EP2330205A1 (en) 2011-06-08

Similar Documents

Publication Publication Date Title
RU2385928C3 (ru) Продуцирование высокоманнозных белков в растительных культурах
RU2009143751A (ru) Производство белков с высоким содержанием маннозы в культуре растительных клеток
Tamaoki et al. Folding motifs induced and stabilized by distinct cystine frameworks.
UA89616C2 (ru) Гидролизат казеина, способ его получения и применения
WO2007064917A3 (en) Methods of preparation of recombinant forms of human beta-amyloid protein and uses of these proteins
WO2005115306A3 (de) Keratin-bindende polypeptide
WO2007002261A3 (en) IL-1β BINDING ANTIBODIES AND FRAGMENTS THEREOF
ES2277363T3 (es) Proteinas antimicrobianas.
WO2003037932A3 (en) Human coagulation factor vii polypeptides
NZ591612A (en) Prevention, treatment and diagnosis of p.gingivalis infection with peptides
Bolscher et al. A one-enzyme strategy to release an antimicrobial peptide from the LFampin-domain of bovine lactoferrin
WO2010007144A3 (en) Mutated netrin-4 proteins, fragments thereof and their uses as drugs
WO2007093848A3 (en) Crystalline enoyl-(acyl-carrier-protein) reductase from heliobacter pylori
EA200901441A1 (ru) Новые молекулы антител и нуклеиновых кислот, связывающиеся с грибным белком теплового шока hsp90
KR100220502B1 (ko) 식물성 독소 겔로닌의 단백질 구조물
WO2006097432A3 (de) Keratin-biktdende desmoplakinpolypeptidsequenzen
EP1390503A4 (en) Novel nucleic acid molecules
WO2001061021A3 (en) Transgenic plants having resistance to a fungal disease
BR0309921A (pt) Toxina relacionada a polipetìdeos, peptìdeo-mimético, anticorpo e ácido nucléico com atividade antimicrobiana e antiviral, bem como seu processo de preparação, sua composição farmacêutica e fabricação do medicamento
ATE521626T1 (de) Fusionsprotein zur inhibierung von gebärmutterhalskrebs
EP1381388A4 (en) PREPARATION OF PEPTIDES IN PLANTS AS N-TERMINAL PROTEIN FUSIONS WITH VIRUS COAT
WO2002098911A3 (en) Insecticidal proteins and synergistic combinations thereof
WO2003082206A3 (en) Multiple sclerosis-related immunoglobulin derived proteins, compositions, methods and uses
WO2002083729A3 (en) Polypeptides derived from amyloid precursor peptide (app) and their uses
CA2530731A1 (en) Peptide having apoptosis-inhibiting activity

Legal Events

Date Code Title Description
TH4A Reissue of patent specification
ND4A Extension of patent duration

Effective date: 20211227