RU2342851C1 - Feed supplement for sucking piglets - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: feed supplement contains bifidobacteria B. bifidum in the form of the bacpreparation Bifidumbacterin - 90 wt % and vegetable supplement - dry powder of stevia leaves and stems - 10 wt %.

EFFECT: normalisation of microbiocenosis caused by malfunction of bifidogenic activity, increasing enzymatic activity and feed assimilability, improving carbohydrate metabolism, increasing productivity characteristics, ensuring ecological health of pigs and environment, increasing feed assimilability, increasing preservation of pig livestock.

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Description

FIELD OF THE INVENTION

The invention relates to livestock, veterinary medicine, crop production, processing of agricultural products, biotechnology and is intended for use in feeding suckling piglets and creating methods for preserving the livestock of farm animals by introducing probiotic and herbal additives into their diet to activate enzymatic, increase bifidogenic activity of the gastrointestinal path, regulation of protein-carbohydrate metabolism at various stages of feeding, acceleration of the adaptation period as well as improving the ecological condition of animals and the environment.

BACKGROUND

Known RF patent No. 2062038, class. А23К 1/16, 1996. Provides for use as a probiotic - a liquid preparation of amylovarin (strain BT - 24/88), containing 1 ml of 100 ml of K.O.E. and administered at the rate of 5-15 ml per head per day.

A known method of obtaining a feed additive, which uses a combination of live cultures of probiotic bacteria Runnmococcus, Lb. Acidophilus in the ratio of wt.%: Uterine culture of bacteria - 15-35, adsorbent - 65-85 (RF patent No. 2190332, CL A23K 1/16, 2002), as well as probiotic feed additives containing animal bifidobacteria and streptococci (Development Research Institute of Agriculture of the North-East, Kirov Region) - streptobifid, lactocide - live cultures of lactic acid bacteria and streptococci grown on milk whey (Development of State Research Institute of Applied Mathematics and Microbiology).

There is data on a producer of a natural sugar substitute (primarily for patients with diabetes mellitus) - a stevia plant (Stevia rebaudiana Bertoni). Biochemical studies of stevia leaves showed that they have a low calorie content and have the property of lowering blood sugar levels (A.A. Krivenko. Prospects for the use of a new sugar-bearing culture (Stevia rebaudiana Bertoni) / A.A. Krivenko, V.I. Zhabina, O .V. Mironova, A.I. Gavrilov, L.D. Ostroukhova //. III international practical conference "The state of the biosphere and human health" - Penza, 2003, pp. 91-93.

The daily intake of dry stevia leaves does not exceed 10 g or 1 g of the substances of the steviazide complex.

Known feed additive from the leaf-stem mass of stevia TU 9759-004-00668620-2007.

Known drug "Bifidumbacterin" FSP 42.0092-2847-02, containing bifidobacteria B. Bifidum, with bifidogenic activity.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is "Method of growing piglets", RF patent No. 2062038, 1996, class. А23К 1/16, which provides for the oral administration of a probiotic in a mixture with piglet feed individually or in a group method during the suction period, obtained on the basis of amylovarin (Lb. Amilovorus) BT - 24/88, containing 100 ml CFU in 1 ml and introduced into the feed from calculation of 5-15 ml per head per day by lubricating the nipples in sows before feeding the newborn.

The disadvantage of this method is that when feeding piglets, only one factor is used that makes it possible to digest food - this is the use of a probiotic amylovarin.

The present invention, in comparison with the prototype and other known technical solutions, has the following advantages: it makes it possible to expand the use of probiotic additives containing bifid bacteria and a vegetable feed additive containing a stevioside complex as the active substance, which allows creating a balanced system that activates reflex functions and provides normobiocenosis of animals .

SUMMARY OF THE INVENTION

The task of the invention is to develop a feed additive for piglets of the suckling period, which could normalize the activity of the gastrointestinal tract by increasing the bifidogenic factor and activate enzymatic activity, carbohydrate metabolism.

The purpose of the invention is the creation of a feed additive for piglets of the suckling period using local probiotics and plant components for the treatment and prevention of non-communicable diseases, normalizing microbiocenosis, activating the enzymatic activity of the gastrointestinal tract, improving carbohydrate metabolism, increasing the gain in live weight, ensuring an increase in the adaptation period of development, increase safety and ensure the improvement of the ecological condition of piglets and the environment of various zones of Stavropolskog edge.

The technical result is achieved using a feed additive, including live cultures of B. bifidum bifidobacteria in the form of the drug "Bifidubacterin" and dry powdery leaf-stem mass from stevia, wt.%:

Bifidubacterin drug 90 Dry powder Stevia leaf mass 10

The essence of the invention lies in the fact that for the preparation of a feed additive, the drug "Bifidubacterin" is mixed with a dry powdered leafy mass of stevia.

Tests show that the proposed feed supplement compensates for the deficiency of useful microorganisms in the diets necessary for the growth and development of piglets, which increase the enzymatic activity of the gastrointestinal tract, improve carbohydrate metabolism, which leads to improved productive indicators

The use of the proposed feed additive for piglets of the suckling period will allow to obtain the effect due to the normalization of microbiocenosis, increased digestibility of feeds by activating the gastrointestinal tract, improving carbohydrate metabolism, which will lead to an increase in live weight, increase preservation and improve the ecological state of piglets and the environment of various zones Stavropol Territory.

DETAILED DESCRIPTION OF THE INVENTION

Examples of specific performance of obtaining a feed additive.

Example No. 1

To prepare 100 kg of a feed additive, the components are mixed, wt.%: The Bifidumbacterin drug - 95.0 with a dry powdery leaf-stem mass from Stevia - 5.0.

Analysis of experimental data on the use of feed additives obtained according to example No. 1 showed an increase in productive indicators: the safety of piglets by 13.7-14.2%, average daily weight gain by 15.7-18.3%, payment of feed by 1.8- 2.3%.

Example No. 2

The feed additive is obtained analogously to example No. 1, but for the preparation of 100 kg of the feed additive is mixed, wt.%: The drug "Bifidumbacterin" - 90.0 with a dry powdery leaf-stem mass from stevia - 10.0.

Analysis of experimental data on the use of feed additives obtained according to example No. 2 showed an increase in productive indicators: the safety of piglets by 23.7-24.8%, average daily weight gain by 25.7-28.3%, payment of feed by 2.3- 2.8%.

Example No. 3

The feed additive is obtained analogously to example No. 1, but for the preparation of 100 kg of the feed additive is mixed, wt.%: The drug "Bifidumbacterin" - 85.0 with a dry powdery leaf-stem mass from stevia - 15.0.

The analysis of experimental data on the use of the feed additive obtained according to example No. 3 showed an increase in productive indicators: the safety of piglets by 23.7-24.8%, average daily weight gain by 25.7-28.3%, payment of feed by 2.3-2 ,8%.

Control group.

The diet of the control group consists without the use of additives.

The invention in comparison with the analogue and other known technical solutions has the following advantages.

The culture of live bifidobacteria B. Bifidum in the form of the drug "Bifidumbacterin" provides:

- improvement of the enzymatic and bifidogenic activity of the gastrointestinal tract, normalization of microbiocenosis.

Maintaining a dry powdery leaf-stem mass from stevia provides:

- regulation of carbohydrate metabolism;

- environmental cleanliness.

In general, the combination of feed additive components provides:

- normalization of microbiocenosis of animals and the environment;

- acceleration of the adaptation period of piglets;

- gain in live weight;

- increase in productive indicators;

- improvement of the ecological state of animals and the environment.

FODDER SUPPLEMENT COMPONENTS FOR PIGLING PIGLING PERIOD

Creating a feed supplement using combinations of components: probiotics based on live biocultures of microorganisms in the form of bacterial preparations for normalizing microbiocenosis, activating the enzymatic activity of the gastrointestinal tract, dry leaf-stem mass from stevia to improve carbohydrate metabolism, increase the gain in live weight, ensure an increase in the adaptation period of development, increase the safety and improve the ecological condition of farm animals and the environment of various zones of Stavr Opole region.

BIOLOGICALLY ACTIVE VEGETABLE COMPONENTS - STEVE SHEET MASS

Inadequate intake of various nutrients by the body negatively affects not only animal health, but also human health, which can be the main cause of human diseases. The natural ingredients of dietary supplements not only make up for the deficiency of feed substances, but also have a positive effect on various systems of the animal organism. Thus, the main purpose of dietary supplements is to provide the body of an animal with nutritional support, prevention, diseases, to help and mobilize the body's defenses to fight diseases, and to support physiological, biological and environmental health.

The normal development of a living organism is a rather complex process and a large number of different components and nutrients are needed to maintain it.

For the normal development of the animal organism, biologically active plant components are necessary.

Plant material is a feed containing fiber and lignin, rich in provitamins, vitamins and BEV - biologically active extractive substances necessary to increase the usefulness of animal feeding diets.

Biologically active substances (BAS) are natural or synthetic products that are used in small doses when fed to farm animals and poultry, which have high biological activity. Dietary supplements are necessary to fill the deficit of certain substances vital to the body of the animal, which they receive in insufficient quantities from fodder. Most feeds do not currently contain many energetically important substances, vitamins, minerals. Long-term storage, the use of preservatives, the use of chemical fertilizers, the artificial acceleration of ripening, non-technological methods of production and processing are factors contributing to the disappearance of essential nutrients from feed products, reducing their feed value, quality and safety.

However, herbal supplements used for fattening piglets do not provide complete assimilation of feed.

Cultivated in the Stavropol Territory, a new technical culture - honey grass - Stevia (Stevia Rebaudiana Bertoni) has biological activity, so it contains active biologically active substances - fat, protein, fiber, minerals - calcium, phosphorus, BEV, carotene.

The composition of the new cultivated sugar-bearing industrial crop - honey grass - Stevia (Stevia Rebaudiana Bertoni) - a potential plant component for feed additives is given in Tables 1, 2, 3, 4.

Organoleptic characteristics of the powder of dry leafy mass from stevia are shown in table 1.

Table 1 The name of indicators Characteristic and norm 1. Appearance A mixture of leaves, stems, buds and flowers with a particle size of from 0.4 to 4 mm 2. The color of the leaf and stems From light green to green. Yellow to brown color allowed 3.Smell Weak, inherent in this raw material, without extraneous odors. 4. Taste Sweet, with a bitter taste

The physico-chemical characteristics of the powder of dry leafy mass from stevia are shown in table 2.

table 2 Name of indicator Mass fraction,% Crude protein, not less than 10,4-11.2 Crude fiber 21.3-24.0 Crude ash not 11.6-12.1 soluble in HCl Crude fat 1.02-1.13 Moisture 8.6-10.12 Calcium 1.08-1.2 Phosphorus 0.87-0.98 Bev 40.8-43.39 Glycosides 4.4-4.6 Carotene 16.25-18.2 Permissible mass fraction of nitrates 200 The amount of exchange energy, MJ 10.68 Number of feed units 0.9

Microbiological indicators, the content of bacterial semen and pathogenic microflora, according to the "Rules for bacteriological research of feed" dry powdered leafy mass from stevia are presented in table 3.

Table 3 microbiological indicators Norm Bako seed, no more than 1 g 5 × 10 ⁵ Pathogenic microflora Not allowed

In order to use stevia as a source of increasing the physiological status of weakened animals and to develop a new generation of biologically active feed additives using plant components, studies were conducted to determine the ecological purity of the stevia plant. It was established that in the leaves and stems of the stevia variety Ramonskaya sweetened, cultivated in the Stavropol Territory, the contents of lead, arsenic, mercury, cadmium, HCH, DDE, DDT, nitrates, etc. are within the levels allowed by SanPiN for plant-based dietary supplements.

The safety indicators of dry powdery leaf-stem mass from stevia are presented in table 4.

Table 4 Indicators Allowed Levels ND on test methods Arsenic, mg / kg, not more than 0.5 GOST 9958-81 Mercury, mg \ kg 0.05 GOST 26927 Lead, mg / kg, no more 3.0 GOST 28875-90 Cadmium, mg / kg, no more 0.3 GOST 28875-90 Radionuclides: cesium - 137 200 Bq / kgMU 5779-91 strontium - 90 one hundred Bq / kgMU 5778-91 General toxicity Not allowed GOST R 52 337 Pesticides ghtst 0.2 GOST 13496.20 DDT and its metabolites 0.05

The content in the leaves of stevia of manganese, copper and zinc, obviously, determine its therapeutic effect in a number of diseases and as a corrector of immunity.

Satisfying the physiological needs of animals in minerals depends on their bioavailability from various feed additives. Vegetable feed consumed by animals only partially satisfies the requirements for some mineral substances, for example, calcium - by 20-30%, phosphorus - by 30-45%. Therefore, for proper feeding, it is necessary to additionally introduce in the diet of animals supplements containing macro- and microelements.

Table 5 shows the microelement composition of the leaves and stems of stevia.

Table 5
Microelement composition of leaves and stems of stevia, mg / kg No.p Name Manganese Zinc Copper one. Leaves 14.0 33.8 7.0 2. The stalks 4.0 7.0 4.2

As a result of studies of the microelement composition of leaves and stems of stevia, data were obtained that the leaves of stevia in comparison with the stems contain 3.5 times more manganese, 4.8 times more zinc, almost 2 times copper.

The leaves of stevia contain a complex of biologically active substances that determine the adaptogenic properties of the human and animal organisms. In the complex of modern problems, stevia has properties useful for the animal organism that are necessary for creating fodder additives of a new generation and can become a promising culture not only in agronomic practice, but also in animal husbandry.

BIOLOGICALLY ACTIVE ADDITIVES IN THE FORM OF LIVING BIOCULTURES OF MICRO-ORGANISMS

The efficiency of animal feed use depends on a number of factors, including the conditions of keeping and feeding, as well as their genetic potential. To partially replace the missing biologically active substances that stimulate the growth, development and vital activity of the body, as well as normalize the biocenosis, it is necessary to use dietary supplements of microbial origin.

In countries with highly developed livestock breeding, probiotics, biologically active living cultures of beneficial microorganisms, are widely used to prevent intestinal dysbiosis of young farm animals and birds.

Interest in probiotics as environmentally friendly drugs has intensified the processes of studying the biological properties of bacteria, the most active in probiotic terms. The most studied and implemented is the creation and use of drugs using microorganisms. One of the classic probiotics is bifidobacterium Bifidobacterium bifidum.

Drugs and dietary supplements produced by biotechnological methods using live microorganisms are used to stimulate non-specific immunity, prevent and treat mixed gastrointestinal infections, digestive disorders resulting from various factors.

Biologically active substances - probiotics are a new class of drugs that affect the body at a systemic level. Their influence affects regulatory systems, due to which nonspecific resistance of the organism, immunity is activated. The use of probiotics allows immunocorrection of the body.

In the Stavropol Territory, the Bifidumbacterin bacterium is produced, containing up to 10 ⁷ cells of Bifidobacterium Bifidum, one of the most active probiotics.

The use of bifidobacteria in the form of the drug "Bifidumbacterin" containing living cells of normal microflora B. Bifidum as a biologically active component of the feed additive for piglets of the suckling period is appropriate for the stimulating effect of the body's protective systems.

The positive effect of bifidoflora on the physiological functions of the body is associated with the production of lactic and acetic acids, which create an acid reaction in the intestine, which prevents the propagation of pathogenic, putrefactive and gas-forming microflora.

In general, a combination of biologically active additives as components of a feed additive for piglets of the suckling period, probiotics - live cultures of bifidobacteria in the form of "Bifidumbacterin" and vegetable - dry leaf-stem mass powder from stevia in their composition provides an increase in reproductive qualities and productive indicators.

Claims (1)

Feed additive for piglets of the suckling period, including live cultures of B. bifidum bifidobacterium in the form of the Bifidumbacterin drug, characterized in that it contains a dry powdery leaf-stem mass from stevia, wt.%:
Bifidumbacterin drug 90 Dry powdery leaf-stem mass from stevia 10