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PT1948816E - Processos melhorados de focalização - Google Patents

Processos melhorados de focalização Download PDF

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Publication number
PT1948816E
PT1948816E PT06817236T PT06817236T PT1948816E PT 1948816 E PT1948816 E PT 1948816E PT 06817236 T PT06817236 T PT 06817236T PT 06817236 T PT06817236 T PT 06817236T PT 1948816 E PT1948816 E PT 1948816E
Authority
PT
Portugal
Prior art keywords
dna
amplicons
beads
homo sapiens
pcr
Prior art date
Application number
PT06817236T
Other languages
English (en)
Portuguese (pt)
Original Assignee
Univ Johns Hopkins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Johns Hopkins filed Critical Univ Johns Hopkins
Publication of PT1948816E publication Critical patent/PT1948816E/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00Drugs for disorders of the cardiovascular system
    • A61P9/10Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01RMEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R31/00Arrangements for testing electric properties; Arrangements for locating electric faults; Arrangements for electrical testing characterised by what is being tested not provided for elsewhere
    • G01R31/327Testing of circuit interrupters, switches or circuit-breakers
    • G01R31/3277Testing of circuit interrupters, switches or circuit-breakers of low voltage devices, e.g. domestic or industrial devices, such as motor protections, relays, rotation switches
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01RMEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R31/00Arrangements for testing electric properties; Arrangements for locating electric faults; Arrangements for electrical testing characterised by what is being tested not provided for elsewhere
    • G01R31/50Testing of electric apparatus, lines, cables or components for short-circuits, continuity, leakage current or incorrect line connections
    • G01R31/52Testing for short-circuits, leakage current or ground faults
    • HELECTRICITY
    • H02GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
    • H02HEMERGENCY PROTECTIVE CIRCUIT ARRANGEMENTS
    • H02H1/00Details of emergency protective circuit arrangements
    • H02H1/0007Details of emergency protective circuit arrangements concerning the detecting means
    • H02H1/0015Using arc detectors
    • HELECTRICITY
    • H02GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
    • H02HEMERGENCY PROTECTIVE CIRCUIT ARRANGEMENTS
    • H02H3/00Emergency protective circuit arrangements for automatic disconnection directly responsive to an undesired change from normal electric working condition with or without subsequent reconnection ; integrated protection
    • H02H3/26Emergency protective circuit arrangements for automatic disconnection directly responsive to an undesired change from normal electric working condition with or without subsequent reconnection ; integrated protection responsive to difference between voltages or between currents; responsive to phase angle between voltages or between currents
    • H02H3/32Emergency protective circuit arrangements for automatic disconnection directly responsive to an undesired change from normal electric working condition with or without subsequent reconnection ; integrated protection responsive to difference between voltages or between currents; responsive to phase angle between voltages or between currents involving comparison of the voltage or current values at corresponding points in different conductors of a single system, e.g. of currents in go and return conductors
    • H02H3/33Emergency protective circuit arrangements for automatic disconnection directly responsive to an undesired change from normal electric working condition with or without subsequent reconnection ; integrated protection responsive to difference between voltages or between currents; responsive to phase angle between voltages or between currents involving comparison of the voltage or current values at corresponding points in different conductors of a single system, e.g. of currents in go and return conductors using summation current transformers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Power Engineering (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Cardiology (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Nanotechnology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
PT06817236T 2005-10-24 2006-10-20 Processos melhorados de focalização PT1948816E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US72923505P 2005-10-24 2005-10-24

Publications (1)

Publication Number Publication Date
PT1948816E true PT1948816E (pt) 2012-03-19

Family

ID=37968413

Family Applications (1)

Application Number Title Priority Date Filing Date
PT06817236T PT1948816E (pt) 2005-10-24 2006-10-20 Processos melhorados de focalização

Country Status (11)

Country Link
US (4) US9360526B2 (de)
EP (2) EP2428579B1 (de)
AT (1) ATE536420T1 (de)
CY (1) CY1112525T1 (de)
DK (1) DK1948816T3 (de)
ES (2) ES2426031T3 (de)
HK (1) HK1121193A1 (de)
PL (1) PL1948816T3 (de)
PT (1) PT1948816E (de)
SI (1) SI1948816T1 (de)
WO (1) WO2007050465A2 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP1712639B1 (de) 2005-04-06 2008-08-27 Maurice Stroun Methode zur Krebsdiagnose mittels Nachweis von DNA und RNA im Kreislauf
ATE536420T1 (de) * 2005-10-24 2011-12-15 Univ Johns Hopkins Verbesserte verfahren für beaming
EP3913375A1 (de) 2006-01-11 2021-11-24 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtungen und verfahren zur verwendung bei der bildung und steuerung von nanoreaktoren
EP2530167A1 (de) 2006-05-11 2012-12-05 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
GB2453173A (en) * 2007-09-28 2009-04-01 Dxs Ltd Polynucleotide primers
EP2315629B1 (de) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Tröpfchenbibliotheken
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US20100041048A1 (en) 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
EP4484577A3 (de) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digitale analytanalyse
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
CA2810931C (en) 2010-09-24 2018-04-17 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
EP3859011A1 (de) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Verfahren zur bildung gemischter tröpfchen
EP3736281A1 (de) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
DE202012013668U1 (de) 2011-06-02 2019-04-18 Raindance Technologies, Inc. Enzymquantifizierung
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP2788500A1 (de) * 2011-12-09 2014-10-15 F.Hoffmann-La Roche Ag Identifizierung von non-respondern auf her2-inhibitoren
US20130309667A1 (en) * 2012-05-15 2013-11-21 Anthony P. Shuber Primers for analyzing methylated sequences and methods of use thereof
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
DE102013211113A1 (de) * 2013-06-14 2014-12-18 Siemens Aktiengesellschaft Verfahren zur kombinierten Quantifizierung und Sequenzierung von mindestens einer Ziel-Nukleinsäure
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
ES2895750T3 (es) 2014-09-15 2022-02-22 Abvitro Llc Secuenciación de alto rendimiento de colecciones de nucleótidos
CN107427823B (zh) * 2015-03-24 2020-07-14 日立金属株式会社 陶瓷蜂窝结构体
WO2016170179A1 (en) * 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
US11085073B2 (en) 2015-04-24 2021-08-10 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
US9938572B1 (en) 2015-09-08 2018-04-10 Raindance Technologies, Inc. System and method for forming an emulsion
CN106929598B (zh) * 2017-05-04 2019-07-05 湖南融健基因生物科技有限公司 一种乳液中进行滚环扩增的数字核酸分析方法
US11701391B2 (en) 2017-10-24 2023-07-18 Dalia ELANI Methods of treating an ischemic disease
EP3853373A4 (de) 2018-09-17 2022-06-22 The University of North Carolina at Chapel Hill Verfahren zur quantifizierung von dns-fragmenten in einer probe nach grösse
US11474109B2 (en) 2018-11-16 2022-10-18 Scintimetrics, Inc. Compositions and methods for controllably merging emulsion droplets and sample analysis
EP3725894A1 (de) 2019-04-16 2020-10-21 Blod Diagnostik GmbH Vorrichtung und verfahren zur nukleinsäurezielanreicherung, -suspension und -quantifizierung
CN114902024A (zh) * 2019-11-15 2022-08-12 闪璨迈璀公司 基于荧光团扩散的组合物和方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
DE10048797A1 (de) * 2000-10-02 2002-04-18 Bayer Ag Wirkstoffhaltige Emulsionen
AUPS298102A0 (en) 2002-06-13 2002-07-04 Nucleics Pty Ltd Method for performing chemical reactions
ES2380893T3 (es) * 2003-01-29 2012-05-21 454 Life Sciences Corporation Amplificación de ácidos nucleicos en emulsión en perlas
EP2918595B1 (de) * 2003-07-05 2019-12-11 The Johns-Hopkins University Verfahren und Zusammensetzungen zur Detektion und Zählung von genetischen Variationen
WO2005073410A2 (en) * 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
CA2566538C (en) * 2004-05-13 2023-03-14 Anita Goel Nano-pcr: methods and devices for nucleic acid amplification and detection
US20060228721A1 (en) * 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
ATE536420T1 (de) 2005-10-24 2011-12-15 Univ Johns Hopkins Verbesserte verfahren für beaming
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays

Also Published As

Publication number Publication date
SI1948816T1 (sl) 2012-04-30
ES2426031T3 (es) 2013-10-18
WO2007050465A2 (en) 2007-05-03
DK1948816T3 (da) 2012-04-02
WO2007050465A3 (en) 2007-10-25
EP1948816A4 (de) 2009-11-04
US20110059435A1 (en) 2011-03-10
ES2381204T3 (es) 2012-05-24
EP2428579B1 (de) 2013-05-29
US20130040300A9 (en) 2013-02-14
EP2428579A1 (de) 2012-03-14
US20200123600A1 (en) 2020-04-23
CY1112525T1 (el) 2015-12-09
US10150991B2 (en) 2018-12-11
US10837050B2 (en) 2020-11-17
ATE536420T1 (de) 2011-12-15
US20160376645A1 (en) 2016-12-29
EP1948816B1 (de) 2011-12-07
HK1121193A1 (en) 2009-04-17
EP1948816A2 (de) 2008-07-30
US9360526B2 (en) 2016-06-07
US20120014879A1 (en) 2012-01-19
PL1948816T3 (pl) 2012-07-31

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