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PL1888636T3 - Sposób oczyszczania przeciwciał - Google Patents

Sposób oczyszczania przeciwciał

Info

Publication number
PL1888636T3
PL1888636T3 PL06753791T PL06753791T PL1888636T3 PL 1888636 T3 PL1888636 T3 PL 1888636T3 PL 06753791 T PL06753791 T PL 06753791T PL 06753791 T PL06753791 T PL 06753791T PL 1888636 T3 PL1888636 T3 PL 1888636T3
Authority
PL
Poland
Prior art keywords
purification
ion exchange
antibodies
immunoglobulin
single step
Prior art date
Application number
PL06753791T
Other languages
English (en)
Other versions
PL1888636T5 (pl
Inventor
Roberto Falkenstein
Burkard Kolb
Maria Smida
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34936911&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL1888636(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of PL1888636T3 publication Critical patent/PL1888636T3/pl
Publication of PL1888636T5 publication Critical patent/PL1888636T5/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
PL06753791.0T 2005-05-25 2006-05-23 Sposób oczyszczania przeciwciał PL1888636T5 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP05011302 2005-05-25
PCT/EP2006/004863 WO2006125599A2 (en) 2005-05-25 2006-05-23 Method for the purification of antibodies
EP06753791.0A EP1888636B2 (en) 2005-05-25 2006-05-23 Method for the purification of antibodies

Publications (2)

Publication Number Publication Date
PL1888636T3 true PL1888636T3 (pl) 2012-02-29
PL1888636T5 PL1888636T5 (pl) 2023-03-06

Family

ID=34936911

Family Applications (1)

Application Number Title Priority Date Filing Date
PL06753791.0T PL1888636T5 (pl) 2005-05-25 2006-05-23 Sposób oczyszczania przeciwciał

Country Status (18)

Country Link
US (3) US20090098660A1 (pl)
EP (2) EP1888636B2 (pl)
JP (1) JP4852602B2 (pl)
KR (1) KR100967778B1 (pl)
CN (1) CN101180317B (pl)
AT (1) ATE525087T1 (pl)
AU (1) AU2006251307B2 (pl)
BR (1) BRPI0610201B8 (pl)
CA (1) CA2607375C (pl)
DK (1) DK1888636T4 (pl)
ES (2) ES2373402T5 (pl)
FI (1) FI1888636T4 (pl)
IL (2) IL186338A (pl)
MX (1) MX2007014269A (pl)
NO (1) NO345362B1 (pl)
PL (1) PL1888636T5 (pl)
TW (2) TWI391399B (pl)
WO (1) WO2006125599A2 (pl)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
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TWI391399B (zh) 2005-05-25 2013-04-01 Hoffmann La Roche 測定溶離多肽之鹽濃度之方法
CN101679509A (zh) 2007-06-01 2010-03-24 弗·哈夫曼-拉罗切有限公司 免疫球蛋白纯化
AR067537A1 (es) 2007-07-17 2009-10-14 Hoffmann La Roche Purificacion de polipeptidos pegilados
CL2008002054A1 (es) * 2007-07-17 2009-05-29 Hoffmann La Roche Metodo para la regeneracion de una columna de cromatografia de intercambio cationico despues de la elusion de eritropoyetina monopeguilada y metodo para obtener una eritropoyetina monopeguilada, incorporando el metodo de regeneracion de la columna de intercambio cationico.
CN102171237A (zh) * 2008-08-14 2011-08-31 默沙东公司 使用蛋白a亲和色谱法纯化抗体的方法
ES2648264T3 (es) 2010-03-10 2017-12-29 F. Hoffmann-La Roche Ag Procedimiento para la purificación de soluciones de inmunoglobulinas
KR20120132529A (ko) * 2010-04-14 2012-12-05 에프. 호프만-라 로슈 아게 면역글로불린 응집물 제거
CN103379949B (zh) * 2010-10-11 2016-09-14 艾伯维巴哈马有限公司 蛋白纯化方法
BR112013012422A2 (pt) 2010-12-21 2016-08-30 Hoffmann La Roche "método para produzir uma preparação de anticorpo e anticorpo anti-her2"
PL2686334T5 (pl) 2011-03-16 2020-10-19 F.Hoffmann-La Roche Ag Chromatografia jonowymienna o ulepszonej selektywności do rozdzielania monomerów, agregatów i fragmentów polipeptydowych przez modulację fazy ruchomej
SG10201605397PA (en) 2011-07-01 2016-08-30 Hoffmann La Roche Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides
US10481164B2 (en) * 2012-03-26 2019-11-19 Amgen Inc. Method for using light scattering in real time to directly monitor and control impurity removal in purification processes
EP3048109A4 (en) * 2013-09-17 2017-04-19 Kaneka Corporation Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom
KR101700580B1 (ko) * 2013-10-30 2017-02-13 (주)셀트리온 양이온 교환 크로마토그래피를 이용한 항체의 아형 분리 방법
MX378451B (es) 2014-11-06 2025-03-11 Hoffmann La Roche Variantes de regiòn fc con uniòn del receptor fc neonatal (fcrn) modificado y mètodos de uso.
GB2539420B (en) * 2015-06-16 2021-01-13 Cytiva Sweden Ab Determination of chromatography conditions
RU2018121657A (ru) * 2015-11-18 2019-12-19 Мерк Патент Гмбх ПРОТИВОПОЛОЖНЫЕ ГРАДИЕНТЫ pH-СОЛЬ ДЛЯ УЛУЧШЕННОГО РАЗДЕЛЕНИЯ БЕЛКОВ
RU2018121653A (ru) * 2015-11-18 2019-12-19 Мерк Патент Гмбх Улучшенное разделение белков при ионообменной хроматографии
CN111902720B (zh) 2018-03-21 2025-02-07 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
WO2019192877A1 (en) * 2018-04-03 2019-10-10 Merck Patent Gmbh Cex chromatography media and low salt elution of target proteins from biopharmaceutical feeds
CN111068361B (zh) * 2018-10-22 2022-02-11 中国石油化工股份有限公司 一种己内酰胺离子交换装置及其再生方法
CN110066314B (zh) * 2019-04-01 2023-10-27 上海药明生物技术有限公司 一种高效提高多聚体分离分辨率的亲和纯化工艺
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
IL294901A (en) * 2020-01-24 2022-09-01 Alkermes Pharma Ireland Ltd Methods of purification
CN115728433B (zh) * 2021-08-27 2024-11-15 联邦生物科技(珠海横琴)有限公司 IgG4型单克隆抗体电荷异质性的离子交换色谱检测方法

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Also Published As

Publication number Publication date
US11034754B2 (en) 2021-06-15
EP1888636B2 (en) 2022-03-30
WO2006125599A2 (en) 2006-11-30
EP2261252A3 (en) 2011-03-23
US20090098660A1 (en) 2009-04-16
CN101180317A (zh) 2008-05-14
US9631007B2 (en) 2017-04-25
ES2373402T5 (es) 2022-09-14
TW200716669A (en) 2007-05-01
MX2007014269A (es) 2008-01-24
WO2006125599A3 (en) 2007-06-21
EP1888636A2 (en) 2008-02-20
DK1888636T3 (da) 2011-11-14
KR20080017322A (ko) 2008-02-26
AU2006251307B2 (en) 2011-08-04
JP4852602B2 (ja) 2012-01-11
KR100967778B1 (ko) 2010-07-05
CN101180317B (zh) 2013-06-19
NO345362B1 (no) 2020-12-28
EP2261252A2 (en) 2010-12-15
ES2373402T3 (es) 2012-02-03
TWI391399B (zh) 2013-04-01
NO20075091L (no) 2008-01-23
TW200946543A (en) 2009-11-16
US20140243508A1 (en) 2014-08-28
IL186338A (en) 2011-01-31
EP1888636B1 (en) 2011-09-21
FI1888636T4 (fi) 2023-01-31
IL206993A0 (en) 2012-01-01
CA2607375A1 (en) 2006-11-30
US20170183394A1 (en) 2017-06-29
IL186338A0 (en) 2008-01-20
CA2607375F (en) 2006-11-30
BRPI0610201A2 (pt) 2010-06-01
BRPI0610201B1 (pt) 2020-12-29
ES2389641T3 (es) 2012-10-30
ES2389641T5 (es) 2021-06-10
PL1888636T5 (pl) 2023-03-06
DK1888636T4 (da) 2022-07-18
TWI320788B (en) 2010-02-21
ATE525087T1 (de) 2011-10-15
JP2008542218A (ja) 2008-11-27
EP2261252B1 (en) 2012-07-25
EP2261252B2 (en) 2020-10-21
CA2607375C (en) 2014-01-21
BRPI0610201B8 (pt) 2021-05-25
AU2006251307A1 (en) 2006-11-30

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