PE20131140A1 - Produccion de una n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada y sus usos - Google Patents
Produccion de una n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada y sus usosInfo
- Publication number
- PE20131140A1 PE20131140A1 PE2013000110A PE2013000110A PE20131140A1 PE 20131140 A1 PE20131140 A1 PE 20131140A1 PE 2013000110 A PE2013000110 A PE 2013000110A PE 2013000110 A PE2013000110 A PE 2013000110A PE 20131140 A1 PE20131140 A1 PE 20131140A1
- Authority
- PE
- Peru
- Prior art keywords
- galns
- enzyme
- column
- sulfatase
- acetylgalactosamine
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/06—Sulfuric ester hydrolases (3.1.6)
- C12Y301/06004—N-Acetylgalactosamine-6-sulfatase (3.1.6.4)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Gastroenterology & Hepatology (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Physical Education & Sports Medicine (AREA)
- Dermatology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
SE REFIERE A UN PROCESO DE PRODUCCION DE UNA N-ACETILGALACTOSAMINA-6-SULFATASA HUMANA (GALNS), ACTIVA ALTAMENTE FOSFORILADA QUE COMPRENDE: A) FILTRACION DE UN MEDIO DE CULTIVO DE LA ENZIMA GALNS SECRETADA POR UNA LINEA DE CELULAS MAMIFERAS QUE EXPRESA EL FACTOR HUMANO 1 MODIFICADOR DE LAS SULFATASAS ( SUMF 1) Y LA ENZIMA GALNS HUMANA RECOMBINANTE UTILIZANDO CARBON ACTIVADO ; B) SE COLOCA EL FILTRADO ANTERIOR EN UNA COLUMNA DE CAPTURA BAJO CONDICIONES TALES QUE LA ENZIMA GALNS QUEDE RETENIDA EN LA COLUMNA; C) SE AJUSTA EL PH DEL PRODUCTO DE LA FILTRACION DEL PASO B) HASTA ALCANZAR UN PH DE 4,5±0,1; D) COLOCAR EL PRODUCTO ANTERIOR EN UNA COLUMNA DE INTERCAMBIO IONICO, REALIZAR EL LAVADO BAJO CONDICIONES ADECUADAS TALES QUE LA ENZIMA GALNS QUEDE RETENIDA EN LA COLUMA.DICHO PROCESO TIENE UNA PUREZA DE 95% Y EL97% DE LA ENZIMA GALNS SE ENCUENTRA EN LA FORMA DEL PRECURSOR. REFERIDA ADEMAS A UNA COMPOSICION FARMACEUTICA.DICHO PROCESO ES UTIL PARA EL TRATAMIENTO DE MUCOPOLISACARIDOSIS TIPO IVa (MPS IVa).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36671410P | 2010-07-22 | 2010-07-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PE20131140A1 true PE20131140A1 (es) | 2013-10-17 |
Family
ID=44543776
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PE2017001626A PE20171799A1 (es) | 2010-07-22 | 2011-07-22 | Formulacion que comprende n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada |
| PE2017001625A PE20171798A1 (es) | 2010-07-22 | 2011-07-22 | Formulacion que comprende n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada |
| PE2013000110A PE20131140A1 (es) | 2010-07-22 | 2011-07-22 | Produccion de una n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada y sus usos |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PE2017001626A PE20171799A1 (es) | 2010-07-22 | 2011-07-22 | Formulacion que comprende n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada |
| PE2017001625A PE20171798A1 (es) | 2010-07-22 | 2011-07-22 | Formulacion que comprende n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada |
Country Status (28)
| Country | Link |
|---|---|
| US (3) | US8765437B2 (es) |
| EP (1) | EP2595650B1 (es) |
| JP (2) | JP6030553B2 (es) |
| KR (2) | KR101949906B1 (es) |
| CN (1) | CN103037895B (es) |
| AR (2) | AR082319A1 (es) |
| AU (1) | AU2011280937B2 (es) |
| BR (1) | BR112013001558B1 (es) |
| CA (2) | CA2805673C (es) |
| CL (2) | CL2013000219A1 (es) |
| CY (1) | CY1118934T1 (es) |
| DK (1) | DK2595650T3 (es) |
| ES (1) | ES2616263T3 (es) |
| HR (1) | HRP20170245T1 (es) |
| HU (1) | HUE033163T2 (es) |
| IL (2) | IL224125B (es) |
| LT (1) | LT2595650T (es) |
| MX (1) | MX341838B (es) |
| PE (3) | PE20171799A1 (es) |
| PL (1) | PL2595650T3 (es) |
| PT (1) | PT2595650T (es) |
| RS (1) | RS55669B1 (es) |
| RU (1) | RU2607376C2 (es) |
| SG (1) | SG187580A1 (es) |
| SI (1) | SI2595650T1 (es) |
| SM (2) | SMT201700106T1 (es) |
| WO (1) | WO2012012718A2 (es) |
| ZA (1) | ZA201300286B (es) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2009205978B2 (en) * | 2008-01-18 | 2014-04-10 | Biomarin Pharmaceutical Inc. | Manufacture of active highly phosphorylated human lysosomal sulfatase enzymes and uses thereof |
| US8956859B1 (en) | 2010-08-13 | 2015-02-17 | Aviex Technologies Llc | Compositions and methods for determining successful immunization by one or more vaccines |
| US20130202633A1 (en) * | 2012-02-07 | 2013-08-08 | Saint Louis University, A Non-Profit Organization | Determination of immunogenic peptides in lysosomal enzymes and induction of oral tolerance |
| KR101380740B1 (ko) | 2012-06-29 | 2014-04-11 | 쉐어 휴먼 제네텍 세러피스, 인코포레이티드 | 이듀로네이트-2-설파타제의 정제 |
| US11529397B2 (en) | 2018-01-22 | 2022-12-20 | Saint Louis University | Method of treating mucopolysaccharidosis type IVA |
| EP2970380B1 (en) * | 2013-03-13 | 2018-05-02 | Shire Human Genetic Therapies, Inc. | Method of characterizing lysosomal enzymes |
| PL2968494T3 (pl) | 2013-03-13 | 2019-04-30 | Seattle Genetics Inc | Filtracja z węglem aktywnym do oczyszczania benzodiazepinowych ADC |
| BR112015028605A8 (pt) | 2013-05-15 | 2019-12-24 | Univ Minnesota | uso de uma composição e uso de um imunossupressor e uma composição |
| WO2014201220A1 (en) * | 2013-06-12 | 2014-12-18 | The Board Of Trustees Of The University Of Illinois | Methods for detecting prostate cancer |
| KR101894762B1 (ko) | 2013-07-12 | 2018-09-05 | 이엠디 밀리포어 코포레이션 | 표적 단백질을 함유하는 샘플로부터 활성탄을 사용하여 바이러스 제거를 결정하는 방법 |
| US20170191041A1 (en) * | 2014-07-11 | 2017-07-06 | Biostrategies LC | Materials and methods for treating disorders associated with sulfatase enzymes |
| US10472615B2 (en) | 2016-01-21 | 2019-11-12 | Saint Louis University | Reduced immunogenic proteins for lysosomal storage disorders |
| US11208632B2 (en) | 2016-04-26 | 2021-12-28 | R.P. Scherer Technologies, Llc | Antibody conjugates and methods of making and using the same |
| WO2019045149A1 (en) * | 2017-08-31 | 2019-03-07 | Green Cross Corporation | PROCESS FOR PURIFYING SULFATASE PROTEIN |
| RU2763990C2 (ru) * | 2020-02-19 | 2022-01-12 | Акционерное общество "ГЕНЕРИУМ" | Клетка, продуцирующая с высокой эффективностью активный белок арилсульфатазу в, и способ получения этой клетки |
| WO2021216460A1 (en) * | 2020-04-19 | 2021-10-28 | Figene, Llc | Gene modified fibroblasts for therapeutic applications |
| WO2023150387A1 (en) * | 2022-02-07 | 2023-08-10 | M6P Therapeutics, Inc. | Compositions comprising acid alpha glucosidase and methods of use thereof |
| TW202403043A (zh) * | 2022-03-18 | 2024-01-16 | 美商健臻公司 | 重組人類酸性鞘磷脂酶醫藥組合物及方法 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4179337A (en) | 1973-07-20 | 1979-12-18 | Davis Frank F | Non-immunogenic polypeptides |
| US4394448A (en) | 1978-02-24 | 1983-07-19 | Szoka Jr Francis C | Method of inserting DNA into living cells |
| US4391904A (en) | 1979-12-26 | 1983-07-05 | Syva Company | Test strip kits in immunoassays and compositions therein |
| US4554101A (en) | 1981-01-09 | 1985-11-19 | New York Blood Center, Inc. | Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity |
| US5186941A (en) | 1983-05-06 | 1993-02-16 | Vestar, Inc. | Vesicle formulation for the controlled release of therapeutic agents |
| JPS62122588A (ja) | 1985-11-22 | 1987-06-03 | Taiyo Fishery Co Ltd | 純コンドロイチナ−ゼの製造法 |
| US6048729A (en) | 1987-05-01 | 2000-04-11 | Transkaryotic Therapies, Inc. | In vivo protein production and delivery system for gene therapy |
| PT101031B (pt) | 1991-11-05 | 2002-07-31 | Transkaryotic Therapies Inc | Processo para o fornecimento de proteinas por terapia genetica |
| US5824784A (en) | 1994-10-12 | 1998-10-20 | Amgen Inc. | N-terminally chemically modified protein compositions and methods |
| TW492882B (en) | 1997-11-28 | 2002-07-01 | Caleb Pharmaceuticals Inc | Cholinergic antagonist plaster composition |
| US6689600B1 (en) * | 1998-11-16 | 2004-02-10 | Introgen Therapeutics, Inc. | Formulation of adenovirus for gene therapy |
| US7083793B2 (en) | 1999-02-26 | 2006-08-01 | Millennium Pharmaceuticals, Inc. | Tango 243 polypeptides and uses thereof |
| US6537785B1 (en) | 1999-09-14 | 2003-03-25 | Genzyme Glycobiology Research Institute, Inc. | Methods of treating lysosomal storage diseases |
| US6261595B1 (en) | 2000-02-29 | 2001-07-17 | Zars, Inc. | Transdermal drug patch with attached pocket for controlled heating device |
| RU2195492C2 (ru) * | 2000-09-04 | 2002-12-27 | Институт физиологии Коми научного центра Уральского отделения РАН | Способ получения пектолитического ферментного препарата |
| WO2002074969A2 (en) | 2001-01-26 | 2002-09-26 | University Of Lausanne | Matrix attachment regions and methods for use thereof |
| KR101035043B1 (ko) * | 2002-07-01 | 2011-05-19 | 아르키온 라이프 사이언씨즈 엘엘씨 | 글루코사민 및 ｎ-아세틸글루코사민의 제조를 위한 물질및 공정 |
| PT1592786E (pt) | 2003-02-11 | 2012-01-19 | Shire Human Genetic Therapies | Diagnóstico e tratamento da deficiência de múltiplas sulfatases e outras utilizando uma enzima geradora de formilglicina (fge) |
| JP2007277094A (ja) * | 2004-06-29 | 2007-10-25 | Chemo Sero Therapeut Res Inst | 改変ダニ主要アレルゲン含有医薬組成物 |
| NZ555206A (en) * | 2004-12-22 | 2010-09-30 | Ambrx Inc | Methods for expression and purification of recombinant human growth hormone |
| US20080003220A1 (en) * | 2006-04-21 | 2008-01-03 | Amgen, Inc. | Buffering agents for biopharmaceutical formulations |
| US20080231439A1 (en) | 2007-03-22 | 2008-09-25 | Inventec Corporation | Activity Detection Circuit for a Storage Device |
| US8420081B2 (en) * | 2007-11-30 | 2013-04-16 | Abbvie, Inc. | Antibody formulations and methods of making same |
| AU2009205978B2 (en) * | 2008-01-18 | 2014-04-10 | Biomarin Pharmaceutical Inc. | Manufacture of active highly phosphorylated human lysosomal sulfatase enzymes and uses thereof |
| US7722865B2 (en) * | 2008-01-18 | 2010-05-25 | Biomarin Pharmaceutical Inc. | Manufacture of active highly phosphorylated human lysosomal sulfatase enzymes and uses thereof |
| EP2196476A1 (en) * | 2008-12-10 | 2010-06-16 | Novartis Ag | Antibody formulation |
-
2011
- 2011-07-21 AR ARP110102649A patent/AR082319A1/es not_active Application Discontinuation
- 2011-07-22 MX MX2013000908A patent/MX341838B/es active IP Right Grant
- 2011-07-22 PE PE2017001626A patent/PE20171799A1/es unknown
- 2011-07-22 CA CA2805673A patent/CA2805673C/en active Active
- 2011-07-22 LT LTEP11751689.8T patent/LT2595650T/lt unknown
- 2011-07-22 SM SM20170106T patent/SMT201700106T1/it unknown
- 2011-07-22 PL PL11751689T patent/PL2595650T3/pl unknown
- 2011-07-22 AU AU2011280937A patent/AU2011280937B2/en active Active
- 2011-07-22 EP EP11751689.8A patent/EP2595650B1/en active Active
- 2011-07-22 US US13/188,907 patent/US8765437B2/en active Active
- 2011-07-22 PE PE2017001625A patent/PE20171798A1/es unknown
- 2011-07-22 CN CN201180035900.4A patent/CN103037895B/zh active Active
- 2011-07-22 CA CA3146151A patent/CA3146151A1/en active Pending
- 2011-07-22 PT PT117516898T patent/PT2595650T/pt unknown
- 2011-07-22 WO PCT/US2011/045011 patent/WO2012012718A2/en active Application Filing
- 2011-07-22 SG SG2013004841A patent/SG187580A1/en unknown
- 2011-07-22 KR KR1020157007141A patent/KR101949906B1/ko active Active
- 2011-07-22 ES ES11751689.8T patent/ES2616263T3/es active Active
- 2011-07-22 HU HUE11751689A patent/HUE033163T2/en unknown
- 2011-07-22 DK DK11751689.8T patent/DK2595650T3/en active
- 2011-07-22 RU RU2013107765A patent/RU2607376C2/ru active
- 2011-07-22 BR BR112013001558-6A patent/BR112013001558B1/pt active IP Right Grant
- 2011-07-22 JP JP2013520881A patent/JP6030553B2/ja active Active
- 2011-07-22 SI SI201131104A patent/SI2595650T1/sl unknown
- 2011-07-22 KR KR1020137004487A patent/KR101598897B1/ko active Active
- 2011-07-22 RS RS20170165A patent/RS55669B1/sr unknown
- 2011-07-22 PE PE2013000110A patent/PE20131140A1/es not_active Application Discontinuation
-
2013
- 2013-01-07 IL IL224125A patent/IL224125B/en active IP Right Grant
- 2013-01-11 ZA ZA2013/00286A patent/ZA201300286B/en unknown
- 2013-01-22 CL CL2013000219A patent/CL2013000219A1/es unknown
-
2014
- 2014-05-19 US US14/281,538 patent/US8940513B2/en active Active
- 2014-12-17 US US14/573,907 patent/US9567572B2/en active Active
-
2016
- 2016-07-15 JP JP2016140213A patent/JP2016178950A/ja not_active Withdrawn
-
2017
- 2017-02-15 HR HRP20170245TT patent/HRP20170245T1/hr unknown
- 2017-02-15 CY CY20171100207T patent/CY1118934T1/el unknown
- 2017-02-16 SM SM201700106T patent/SMT201700106B/it unknown
- 2017-03-02 CL CL2017000498A patent/CL2017000498A1/es unknown
-
2018
- 2018-06-26 IL IL260282A patent/IL260282B/en active IP Right Grant
-
2020
- 2020-07-31 AR ARP200102165A patent/AR119552A2/es unknown
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PE20131140A1 (es) | Produccion de una n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada y sus usos | |
| PH12015502408A1 (en) | Purification of iduronate-2-sulfatase | |
| FR2896511B1 (fr) | Procede de culture de cellules issues du tissu adipeux et leurs applications. | |
| EA201171281A1 (ru) | Производство инкапсулированных наночастиц в коммерческих масшатабах | |
| MA46140A (fr) | Facteur de transcription nterf241 et ses procédés d'utilisation | |
| EA201171285A1 (ru) | Новая технология изготовления диклофенака | |
| MY194587A (en) | Composition and method for the diagnosis and treatment of diseases associated with neurite degeneration | |
| MX2015008875A (es) | Metodos para la purificacion de arilsulfatasa a. | |
| EA201171284A1 (ru) | Новая технология изготовления напроксена | |
| MX2012008932A (es) | Proceso para la integracion de calor para la produccion de etanol y proceso de purificacion. | |
| CN104448019B (zh) | 高纯普鲁兰多糖提取工艺 | |
| BR112014017215B8 (pt) | Método para produzir um produto químico | |
| CN104479038B (zh) | 普鲁兰多糖提纯工艺 | |
| AU2012297573A8 (en) | Method for the recovery of magnesium sulphate and production of magnesium oxide | |
| WO2016154046A3 (en) | Biological methods for preparing 3-hydroxypropionic acid | |
| CN101066907A (zh) | 医用甘油的提炼 | |
| RU2014107682A (ru) | Способ получения миобластов, использование биоптата десны, препарат миобластов для лечения патологий мышечной ткани и способ его получения | |
| WO2014020517A3 (en) | Production of extracellular tyrosinase enzyme using the fungus gliocephalotrichum for various applications | |
| FI20115497A7 (fi) | Menetelmä ja laitteisto hydroksikarboksyylihappotuotteen erottamiseksi | |
| KR20130133433A (ko) | 순수 그래핀 대량 제조 방법 i | |
| MD4504B1 (ro) | Procedeu de obţinere a acidului tartric din tartratul de calciu obţinut din deşeuri vinicole | |
| WO2015166334A3 (es) | Enzima esterasa modificada enantioselectiva y proceso para la obtención de la misma | |
| RU2013140653A (ru) | Конъюгат наноалмаза с глицином и способ его получения | |
| LV13889A (lv) | Kongruentais bioetanola dehidratēšanas paņēmiens | |
| RU2012141695A (ru) | Способ подготовки воды для полива |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FC | Refusal |