OA19296A - Conjugate of salicylic acid and peptide. - Google Patents

Abstract

The present invention relates to an antibacterial, anti-inflammatory, or antioxidant composition and, more specifically, to a compound having a structure in which salicylic acid is linked to a peptide via a covalent linkage, and to an antibacterial, anti-inflammatory, or antioxidant pharmaceutical or cosmetic composition containing the compound. The compound having a structure in which salicylic acid is linked to a peptide via a covalent linkage, of the present invention, has excellent physiological activity, such as antibacterial, anti-inflammatory, or antioxidant activity, as well as excellent characteristics, such as solubility in water, and thus the compound can be favorably used in various fields of food, drug, or cosmetics.

Description

[0001] The présent disclosure relates to a compound having a structure in which salicylic acid is linked to a peptide via a covalent bond, and use thereof.

BACKGROUND ART

[0002] Salicylic acid is a compound having a Chemical formula of C7H6O3 which corresponds to O-oxybenzoic acid. Since salicylic acid has various physiological activities such as antibacterial, anti-inflammatory, or antioxidant activity as well as antipyretic or analgésie activity, it is widely used in foods, drugs, cosmetics, etc. For example, salicylic acid is used as an exfoliating agent, a hair-conditioning agent, an anti-dandruff agent, or a skin-conditioning agent in cosmetic formulations, and salicylic acid dérivatives are known to be used as preservatives, UV absorbers, fragrances, solvents, etc. in cosmetics (Korean Patent Publication No. 10-2009-0004980). Further, salicylic acid and dérivatives thereof hâve biological effects on the skin, and specifically, hâve been used to improve major clinical symptoms of skin aging such as fine lines and wrinkles, breakdown of skin tissue, changes in skin color, and loss of skin firmness and tension (Korean Patent Publication No. 10-2000-0017297).

[0003] However, use of salicylic acid and dérivatives thereof may cause tingling, itching, and tightness which cause considérable discomfort, after application to the skin, and thus users with sensitive skin often suffer damage when they use such compounds. In addition, since salicylic acid has very low solubility in water, it is necessary to add various organic solvents in order to solubilize it, which may make compositions containing salicylic acid more inconvénient.

[0004] Accordingly, there is a need to develop a novel compound which may improve the problems of salicylic acid, specifically, low solubility in water, and may further enhance physiological efficacy of salicylic acid.

DESCRIPTION OF EMBODIMENTS TECHNICAL PROBLEM

[0005] To improve the existing problems of salicylic acid, an object of the présent disclosure is to provide a substance having excellent characteristics, such as solubility in water, while having physiological activity équivalent to or superior to that of a natural form of salicylic acid.

SOLUTION TO PROBLEM

[0006] To achieve the above object, the présent disclosure provides a compound having a structure in which salicylic acid is linked to a peptide via a covalent linkage.

[0007] According to one embodiment of the présent disclosure, the peptide may consist of 2 to 30 amino acid sequences, 5 to 20 amino acid sequences, 8 to 15 amino acid sequences, or 10 to 12 amino acid sequences, but is not limited thereto.

[0008] According to another embodiment of the présent disclosure, the peptide may be a water-soluble peptide, but is not limited thereto. According to an embodiment of the présent disclosure, in the water-soluble peptide, a proportion of amino acids having hydrophilic side chains may be as high as 50% or more, 60% or more, 70% or more, 80% or more, 90% or more, or 100%. According to another embodiment ofthe présent disclosure, in the water-soluble peptide, the number of amino acids having hydrophobie side chains may be five or less, four or less, three or less, two or less, or one, or none.

[0009] According to still another embodiment of the présent disclosure, the peptide may be a peptide consisting of an amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 4, but is not limited thereto.

[0010] Further, the présent disclosure provides an antibacterial, anti-inflammatory, or antioxidant pharmaceutical composition, the composition including any one of the compounds described above.

[0011] Further, the présent disclosure provides an antibacterial, anti-inflammatory, or antioxidant cosmetic composition, the composition including any one ofthe compounds described above.

[0012] According to one embodiment of the présent disclosure, the cosmetic composition may hâve a formulation such as a softener, a nourishing toner, a nutrient cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a facial mask, a spray, a powder, a hair tonie, a haïr cream, a haïr lotion, a hair shampoo, a hair rinse, a hair conditioner, a hair spray, a hair aérosol, a pomade, a sol gel, an émulsion, an oil, a wax, or an aérosol, but is not limited thereto.

ADVANTAGEOUS EFFECTS OF DISCLOSURE

[0013] A compound having a structure, in which salicylic acid is linked to a peptide via a covalent linkage, ofthe présent disclosure has excellent physiological activity, such as antibacterial, anti-inflammatory, or antioxidant activity, as well as excellent characteristics, such as solubility in water, and thus the compound may be usefully applied to various fields of foods, drugs, cosmetics, etc.

BRIEF DESCRIPTION OF DRAWINGS

[0014] FIG. 1 shows an image showing solubility of compounds of the présent disclosure and salicylic acid in water;

[0015] FIG. 2A shows immunostaining images showing the morphology and the number of kératinocytes after treatment with the compounds of the présent disclosure and salicylic acid, respectively, and FIG. 2B is a graph showing the relative number of kératinocytes according to treatment concentrations ofthe compounds;

[0016] FIGS. 3A and 3B show images and graphs showing antibacterial activity against Propionibacterium acnés after treatment with the compounds of the présent disclosure and salicylic acid, respectively;

[0017] FIGS. 4A and 4B show results of fluorescence-activated cell sorting (FACS) and a graph showing relative levels of intracellular reactive oxygen species (ROS), respectively, indicating effects ofthe compounds ofthe présent disclosure and salicylic acid on human hair dermal papilla cells (HHDPCs);

[0018] FIGS. 5A and 5B show results of FACS and a graph showing relative levels of intracellular ROS, respectively, indicating effects of the compounds of the présent disclosure and salicylic acid on NIH3T3 fibroblasts;

[0019] FIGS. 6A and 6B show results of FACS and a graph showing relative levels of intracellular ROS, respectively, indicating effects of the compounds of the présent disclosure and salicylic acid on HaCaT kératinocytes;

[0020] FIGS. 7A and 7B show results of immunohistochemical staining and RT-PCR, indicating effects of the compounds of the présent disclosure and salicylic acid on extracellular matrix expression in HaCaT kératinocytes, respectively; and

[0021] FIG. 8 shows an electrophoresis image showing effects of the compounds of the présent disclosure and salicylic acid on Propionibacterium acnes-induced inflammatory cytokine expression in HaCaT kératinocytes.

BEST MODE

[0022] To achieve the above objects, the présent disclosure provides a compound having a structure, in which salicylic acid is linked to a peptide via a covalent linkage.

[0023] The salicylic acid may be 2-hydroxybenzoic acid having a Chemical formula of CzHôOs, and may hâve a Chemical structure represented by the following formula.

O

[0024] ^0H

[0025] In the présent disclosure, the term “peptide” means a linear molécule formed by amino acid residues which are linked to each other via a peptide bond. The peptide may be prepared according to a common biological or Chemical synthesis method known in the art, specifically, solid-phase synthesis techniques (Merrifield, J. Amer. Chem. Soc. 85:2149-54(1963); Stewart, et al., Solid Phase Peptide Synthesis, 2nd. ed., Pierce Chem. Co.: Rockford, 111(1984)).

[0026] The peptide may be to enhance solubility of salicylic acid, and in this regard, the peptide may be a water-soluble peptide, but is not limited thereto. According to one embodiment of the présent disclosure, the peptide may consist of 2 to 30 amino acid sequences, 5 to 20 amino acid sequences, 8 to 15 amino acid sequences, or 10 to 12 amino acid sequences. According to an embodiment of the présent disclosure, in the peptide, a proportion of amino acids having hydrophilic side chains may be as high as 50% or more, 60% or more, 70% or more, 80% or more, 90% or more, or 100%. According to another embodiment of the présent disclosure, in the peptide, a proportion of amino acids having hydrophobie side chains may be as low as less than 50%, 40% or less, 30% or less, 20% or less, 10% or less, or 0%. As used herein, the “amino acids having hydrophilic side chains” represent arginine (Arg), histidine (His), lysine (Lys), aspartic acid (Asp), glutamic acid (Glu), serine (Ser), threonine (Thr), asparagine (Asn), glutamine (Gin), cysteine (Cys), selenocysteine (Sec), glycine (Gly), and proline (Pro), and the “amino acids having hydrophobie side chains” represent alanine (Ala), valine (Val), isoleucine (Ile), leucine (Leu), méthionine (Met), phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp), but are not limited thereto. In addition to the abovementioned amino acids which exist in nature, variants thereof, etc. may be used without limitation. According to an embodiment of the présent disclosure, in the peptide, the number of amino acids having hydrophobie side chains may be five or less, four or less, three or less, two or less, or one, or may be none. According to an embodiment of the présent disclosure, the peptide may be a peptide consisting of an amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 4, but is not limited thereto.

[0027] According to an embodiment of the présent disclosure, the compound of the présent disclosure may hâve excellent solubility in water (see FIG. 1B), and may also hâve a kératinocyte growth-stimulating ability (see FIGS. 2A and 2B). According to another embodiment of the présent disclosure, the compound of the présent disclosure may hâve antibacterial activity against Propionibacterium acnés (see FIGS. 3A and 3B), and antioxidant activity on various cell lines (see FIGS. 4A to 6B). According to still another embodiment ofthe présent disclosure, the compound ofthe présent disclosure may increase expression of extracellular matrix secreted from cells (see FIGS. 7A and 7B), and may hâve inhibitory activity on Propionibacterium acnes-induced inflammatory cytokine expression (see FIG. 8).

[0028] The compound of the présent disclosure as it is may be very stable, but its stability may be further improved by modifying any amino acid constituting the peptide linked to the compound. According to one embodiment of the présent disclosure, the N-terminus of the peptide may be bound with a protecting group selected from the group consisting of an acetyl group, a fluorenyl methoxy carbonyl group, a formyl group, a palmitoyl group, a myristyl group, a stearyl group, and polyethylene glycol (PEG), thereby further improving the stability. According to another embodiment of the présent disclosure, the peptide may be bound with a protecting group selected from the group consisting of an acetyl group, a fluorenyl methoxy carbonyl group, a formyl group, a palmitoyl group, a myristyl group, a stearyl group, and polyethylene glycol (PEG), thereby further improving the stability.

[0029] The above-described amino acid modification may function to greatly improve stability of the compound of the présent disclosure. As used herein, the term “stability” is used to include the meaning of “in vitro” stability such as storage stability (e.g., roomtemperature storage stability) as well as “in vivo” stability. Further, the above-described protecting group may serve to protect the compound of the présent disclosure from attack of proteases in vivo and in vitro.

[0030] Further, the présent disclosure provides an antibacterial, anti-inflammatory, or antioxidant composition including the compound as an active ingrédient. According to still another aspect of the présent disclosure, the présent disclosure provides a composition for improving skin conditions, the composition including the compound as an active ingrédient. In the présent disclosure, the composition may be in the form of a pharmaceutical composition, a health food, or a cosmetic composition, but is not limited thereto. Further, according to an embodiment of the présent disclosure, the improvement of skin conditions by the compound of the présent disclosure may include wrinkle improvement, skin elasticity improvement, skin aging prévention, skin moisturizing improvement, wound repair, or skin régénération, but is not limited thereto.

[0031] Since the composition of the présent disclosure includes the above-described compound of the présent disclosure as an active ingrédient, descriptions common to the two are omitted to avoid the excessive complexity of the présent disclosure.

[0032] According to an embodiment of the présent disclosure, the composition of the présent disclosure is a pharmaceutical composition including (a) a pharmaceutically effective amount of the above-described compound of the présent disclosure; and (b) a pharmaceutically acceptable carrier.

[0033] In the présent disclosure, the term “pharmaceutically effective amount” means an amount which is sufficient to achieve the above-described efficacy or activity of the compound ofthe présent disclosure.

[0034] The pharmaceutically acceptable carrier included in the pharmaceutical composition of the présent disclosure may include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methylcellulose, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnésium stéarate, minerai oil, etc. which are commonly used upon formulating, but is not limited thereto. The pharmaceutical composition of the présent disclosure may further include a lubricant, a wetting agent, a sweetener, a flavor, an emulsifier, a suspending agent, a preservative, etc., in addition to the above ingrédients. An appropriate pharmaceutically acceptable carrier and formulation are described in detail in Remington’s Pharmaceutical Sciences (19th ed„ 1995).

[0035] The pharmaceutical composition of the présent disclosure may be formulated in a unit dosage form or into a multidose container using a pharmaceutically acceptable carrier and/or excipient according to a method that may be easily carried out by those skilled in the art to which the présent disclosure pertains. In this regard, the formulation may be in the form of a solution, a suspension, or an émulsion in an oily or aqueous medium, or in the form of an extract, a powder, granules, a tablet, a capsule, or a gel (e.g., a hydrogel), and may further include a dispersing agent or a stabilizing agent.

[0036] The pharmaceutical composition according to the présent disclosure may be administered orally or parenterally at the time of clinical administration, and may be used in the form of a general pharmaceutical préparation. That is, the pharmaceutical composition ofthe présent disclosure may be administered in various forms for oral and parentéral administration at the time of practical clinical administration. The formulation may be prepared using a commonly used diluent or excipient, such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, etc. Solid formulations for oral administration may include a tablet, a pill, a powder, granules, a capsule, etc., and these solid formulations may be prepared by mixing a herbal extract or a herbal fermented product with at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, etc. In addition to the excipient, a lubricant such as magnésium stéarate, talc, etc. may also be used. Liquid formulations for oral administration may include a suspension, a liquid formulation for internai use, an émulsion, a syrup, etc. In addition to a commonly used diluent such as water and liquid paraffin, various excipients, for example, a wetting agent, a sweetener, an aromatic, a preservative, etc. may also be included. Formulations for parentéral administration may include a sterilized aqueous solution, a non-aqueous solvent, a suspension, an émulsion, a lyophilized formulation, and a suppository. The non-aqueous solvent or suspension may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, etc. As a base of the suppository, witepsol, macrogol, tween 61, cocoa butter, laurin butter, glycerol, gelatin, etc. may be used.

[0037] Dosage units may contain, for example, 1, 2, 3, or 4 single doses, or one-half, one-third, or one-fourth of a single dose. A single dose contains an amount of the active drug administered once, and generally corresponds to a whole, half, third, or quarter of the daily dosage.

[0038] The pharmaceutical composition ofthe présent disclosure may be formulated in a unit dosage form or into a multidose container using a pharmaceutically acceptable carrier and/or excipient according to a method that may be easily carried out by those skilled in the art to which the présent disclosure pertains. In this regard, the formulation may be in the form of a solution, a suspension, or an émulsion in an oily or aqueous medium, or in the form of an extract, a powder, granules, a tablet, a capsule, or a gel (e.g., a hydrogel), and may further include a dispersing agent or a stabilizing agent.

[0039] According to an embodiment of the présent disclosure, the composition of the présent disclosure may be a cosmetic composition including (a) a cosmetically effective amount of the above-described compound of the présent disclosure; and (b) a cosmetically acceptable carrier.

[0040] In the présent disclosure, the term “cosmetically effective amount” means an amount which is sufficient to achieve the above-described skin-improving efficacy of the compound ofthe présent disclosure.

[0041] The cosmetic composition of the présent disclosure may be prepared into any formulation common in the art, and for example, prepared into a solution, a suspension, an émulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleanser, an oil, a powder foundation, an émulsion foundation, a wax foundation, a spray, etc., but is not limited thereto. More specifically, the cosmetic composition may be prepared into various formulations such as a softener, a nourishing toner, a nutrient cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a facial mask, a spray, a powder, a hair tonie, a hair cream, a hair lotion, a hair shampoo, a hair rinse, a hair conditioner, a hair spray, a hair aérosol, a pomade, a solution such as a gel, a sol gel, an émulsion, an oil, a wax, an aérosol, etc., but is not limited thereto.

[0042] When the formulation of the présent disclosure is a paste, a cream, or a gel, an animal oil, a plant oil, wax, paraffin, starch, tragacanth, cellulose dérivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be used as a carrier component.

[0043] When the formulation of the présent disclosure is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, when the formulation of the présent disclosure is a spray, it may further include a propellant such as chlorofluorohydrocarbon, propane/butane, or dimethyl ether, but is not limited thereto.

[0044] When the formulation of the présent disclosure is a solution or an émulsion, a solvent, a solubilizer or an emulsifier may be used as a carrier component. For example, water, éthanol, isoproanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol fatty ester, or fatty acid ester of polyethylene glycol or sorbitan may be used, but is not limited thereto.

[0045] When the formulation of the présent disclosure is a suspension, a liquid diluent such as water, éthanol, or propylene glycol; a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester;

microcrystalline cellulose; aluminum metahydroxide; bentonite; agar; tragacanth, etc. may be used as a carrier component, but is not limited thereto.

[0046] When the formulation of the présent disclosure is a surfactant-containing cleanser, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium dérivatives, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin dérivatives, ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component, but is not limited thereto.

[0047] When the formulation of the présent disclosure is a haïr shampoo, the compound ofthe présent disclosure may be mixed with base components for shampoo, such as a thickener, a surfactant, a viscosity adjuster, a moisturizer, a pH adjuster, a preservative, an essential oil, etc. As the thickener, CDE may be used. As the surfactant, LES which is an anionic surfactant and coco betaine which is an amphoteric surfactant may be used. As the viscosity adjuster, polyquater may be used. As the moisturizer, glycerin may be used. As the pH adjuster, citric acid or sodium hydroxide may be used. As the preservative, a grapefruit extract may be used. In addition, essential oils (e.g., cedarwood, peppermint, rosemary, etc.), silk amino acids, panthenol, and vitamin E may be added. According to an embodiment of the présent disclosure, when the compound ofthe présent disclosure is regarded as 100 parts by weight, 5 to 10 parts by weight of CDE; 30 to 40 parts by weight of LES; 10 to 20 parts by weight of coco betaine; 0.1 to 0.2 parts by weight of polyquater; 5 to 10 parts by weight of glycerin; 0.1 to 1.01 parts by weight of grapefruit extract; 0.5 to 1 part by weight of silk amino acid; 0.5 to 1 part by weight of panthenol; 0.5 to 2 parts by weight of vitamin E; and 0.01 to 0.1 part by weight of at least one of cedarwood, peppermint, and rosemary as the essential oil may be mixed, but is not limited thereto.

[0048] The components included in the cosmetic composition ofthe présent disclosure may include components commonly used in cosmetic compositions, in addition to the compound of the présent disclosure as an active ingrédient and the carrier component, and may include, for example, common additives such as an antioxidant, a stabilizer, a solubilizer, a vitamin, a pigment, and a fragrance, but is not limited thereto.

[0049] Hereinafter, the présent disclosure will be described in detail with reference to

Examples.

[0050] However, these Examples are for illustrative purposes only, and the content of the présent disclosure is not intended to be limited by the following Examples.

[0051] Example 1. Synthesis of compounds of the présent disclosure

[0052] <1 -1 > Synthesis of peptides

[0053] <1-1 ~1> Synthesis of peptide of SEQ ID NO: 1

[0054] 700 mg of chlorotrityl chloride resin (CTL resin, Nova biochem [0064] Cat No. 01-64-0021) was added to a reactor, and 10 ml of methylene chloride (MC) was added thereto, followed by stirring for 3 minutes. The solution was removed, and 10 ml of dimethylformamide (DMF) was added, and stirred for 3 minutes to remove the solvent. 10 ml of a dichloromethane solution was added to the reactor, and 200 mmole of FmocHis(Trt)-OH (Bachem, Swiss) and 400 mmole of diisopropylethylamine (DIEA) were added thereto, and dissolved well by stirring, and allowed to react under stirring for 1 hour. After reaction, washing was carried out, and methanol and DIEA (2:1) were dissolved in dichloromethane (DCM) and allowed to react for 10 minutes, followed by washing with an excessive amount of DCM/DMF (1:1). The solution was removed, 10 ml of DMF was added, and stirred for 3 minutes. Then, the solvent was removed again.

ml of a deprotecting solution (20% piperidine/DMF) was added to the reactor, and stirred at room température for 10 minutes. Then, the solution was removed. The équivalent amount of the deprotecting solution was added, and allowed to react for 10 minutes. Then, the solution was removed, and washing was carried out with DMF twice, with MC once, and with DMF once each for 3 minutes. Thus, a His(Trt)-CTL resin was prepared.

[0055] 10 ml of DMF solution was added to a new reactor, and 200 mmole of FmocThr(tBu)-OH(Bachem, Swiss), 200 mmole of HoBt, and 200 mmole of Bop were added and dissolved well by stirring. 400 mmole of DIEA was added in two fractions to the reactor, and stirred for at least 5 minutes until ail solids were dissolved. The mixed solution in which the amino acids were dissolved was added to the reactor containing the deprotected resin, and allowed to react at room température for 1 hour under stirring. The reaction solution was removed, and stirring was performed with DMF solution three times each for 5 minutes, followed by removing. A small amount of the reaction resin was taken, and reactivity thereof was examined by a Kaiser test (a ninhydrin Test). Deprotection reaction was allowed twice using the deprotecting solution in the same manner as above to préparé a Thr(tBu)-His(Trt)-CTL resin. Sufficient washing was carried out with DMF and MC, and the Kaiser test was further performed. Then, the following amino acid attachment test was performed in the same manner as above.

[0056] In accordance with the selected amino acid sequence, Fmoc-Trp, Fmoc-Gly, Fmoc(Gly), Fmoc-Lys(Boc), Fmoc-Lys(Boc), Fmoc-Ser(tBu), Fmoc-Lys(Boc), and Fmoc-Tyr(tBu) were serially reacted in this order. Fmoc-protecting group was reacted with the deprotecting solution twice each for 10 minutes, and then washed well and removed. Acetic anhydride and DIEA, HoBt were added and allowed to acetylate for 1 hour, and then the prepared peptidyl resin was washed with DMF, MC, and methanol each three times, and nitrogen gas was slowly applied to dry the resin. Then, the resin was completely dried under vacuum in the presence of P2O5. 30 ml of a leaving solution [95% trifluoroacetic acid, 2.5% distilled water, and 2.5% thioanisole] was added, and then reacted at room température for 2 hours with intermittent agitating. The resin was filtered, and washed with a small amount of TFA solution, after which the filtrate was combined with the mother liquor. After distillation under reduced pressure to reduce the total volume by half, précipitation was induced using 50 ml of cold ether, and the formed precipitate was collected by centrifugation, followed by washing twice with cold ether. After removing the mother liquor, the résultant was sufficiently dried under nitrogen atmosphère to synthesize 1.15 g of unpurified Ac-YKSKKGGWTH peptide (SEQ ID NO: 1) (yield 89.5%). A molecular weight thereof was measured as 1233.3 (a theoretical value: 1233.4) by a molecular weight analyzer.

[0057] <1-1-2> Synthesis of peptides of SEQ ID NO: 2 to SEQ ID NO: 4

[0058] A peptide of SEQ ID NO: 2 (Tyr-lle-Ser-Lys-Lys-His-Ala-Gly-Lys-Asn-Trp-Phe: YISKKHAGKNWF), a peptide of SEQ ID NO: 3 (Lys-Leu-Lys-Lys-Thr-Glu-Thr-GIn: KLKKTETQ), and a peptide of SEQ ID NO: 4 (Glu-Leu-lle-Glu-His-Gly-Gly-Gly-Arg-ProAla-Asp: ELIEHGGGRPAD) were synthesized in the same manner as in Example <1-11>.

[0059] [Table 11

SEQ ID NO	Amino acid sequence	Analytical value (molecular weight analyzer)
Analytical value	Theoretical value
1	Ac-YKSKKGGWTH	1233.3	1233.4
2	YISKKHAGKNW	1478.8	1478.7
3	KLKKTETQ	975.1	975.1
4	ELIEHGGGRPAD	1250.9	_______1250.35

[0060] <1-2> Synthesis of compounds ofthe présent disclosure

[0061] To a peptide reactor, the peptidyl resin (1 mmol) and 10 ml of 1-methyl-2pyrrolidone (NMP) were added, and 270 mg (2.0 equiv.) of 1-hydroxybenzotriazole (HOBt) and 759 mg (2.0 equiv.) of N,N,N’,N’-tetramethyl-O-(1H-benzotriazol-1yl)uronium hexafluorophosphate or O-(benzotriazol-1-yl)-N,N,N’,N’-tetramethyl uronium hexafluorophosphate, and 277 mg (2.0 equiv.) of salicylic acid were added and allowed to react for 30 minutes. 388 mg (3 equiv.) of Ν,Ν-diisopropylethylamine (DIEA) was added and reacted at room température for 24 hours to 72 hours, and filtration was carried out to obtain a reacted peptidyl resin. The obtained resin was reacted with a cleavage solution at room température for 2 hours to remove the resin and the protecting group. Recrystallization was carried out using 10 ml (10 mmol) of diethyl ether to obtain a hybrid peptide.

[0062] Experimental Example 1. Test of solubilitv of compounds of the présent disclosure

[0063] The salicylic acid-peptide 1 compound (compound 1), the salicylic acid-peptide 2 compound (compound 2), the salicylic acid-peptide 3 compound (compound 3), and the salicylic acid-peptide 4 compound (compound 4) prepared in Example <1-2> and salicylic acid were dissolved in distilled water at a concentration of 10 mg/ml, respectively.

[0064] As a resuit, it was confirmed that salicylic acid as it is was hardly dissolved in water, whereas ail of compound 1 to compound 4 of the présent disclosure were completely dissolved in water (FIG. 1)

[0065] Experimental Example 2. Effects of compounds of the présent disclosure on kératinocyte qrowth

[0066] To analyze growth factor-like efficacy and inhibitory efficacy of the compounds synthesized in Example <1-2>, a sulforhodamine B (SRB Sigma) colorimétrie assay was performed using a HaCaT kératinocyte cell line (Korean Cell Line Bank) in accordance with a method of Rizzino, et al. (Cancer Res. 48:4266(1988))

[0067] HaCaT kératinocyte cell line was seeded in a 96-well plate at a density of 3,000 cells perwell, and then cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) containing 10% fêtai bovine sérum (FBS, Sigma) for 24 hours under conditions of 37°C and 5% CO₂. The cultured cell line was detached from the bottom of the culture plate using a 1% trypsin solution, and only cell precipitate was collected by centrifugation. The cell precipitate was suspended in DMEM without FBS, and then cultured for 24 hours under conditions of 37°C and 5% CO2. 24 hours later, the medium was replaced by the same medium from which sérum was completely removed. A blank sample in which the cell precipitate was dissolved stérile in 10% DMSO was prepared for standardization. Each of the compound 1 to the compound 4 (50 μΜ) of the présent disclosure, salicylic acid (50 μΜ), and EGF (100 nM) as a positive control were treated. Incubation was carried out under the same conditions as above for 72 hours. After completing the incubation, each culture supernatant was removed and cells were fixed with éthanol and washed with phosphate buffer saline (PBS) three times. After removing the washing solution, the cells were treated with a colorimétrie SRB solution, and then sufficiently washed with 1% acetic acid. The cells were observed under a microscope to examine living cells. Absorbance at UV 560 nm was measured to détermine cell viability.

[0068] 72 hours after treâting kératinocytes with the compound of the présent disclosure as above, changes in cell morphology were observed under a microscope. As a resuit, it was confirmed that the compounds of the présent disclosure changed growth and morphology of kératinocytes (FIG. 2A). Further, salicylic acid used as the positive control inhibited growth of kératinocytes due to toxicity, whereas the compounds of the présent disclosure greatly increased growth of kératinocytes (FIG. 2B).

[0069] Experimental Example 3. Antibacterial effects of compounds of the présent disclosure

[0070] Antibacterial effects of the compounds synthesized in Example <1-2> were examined using Propionibacterium acnés. To this end, Propionibacterium acnés was first cultured on an agar plate. Paper dises were soaked with each 200 mM of the compound 1 to the compound 4 synthesized in Example <1-2> and salicylic acid, and then the paper dise soaked with the sample was placed on the agar plate on which Propionibacterium acnés had been cultured, followed by incubation. 3 days later, the size of a clear zone with no microbial growth was measured to détermine the antibacterial effect of each compound.

[0071] As a resuit, it was confirmed that the compounds of the présent disclosure did not reduce the antibacterial effect of salicylic acid used as the positive control, and in particular, the compound 1 and the compound 3 showed remarkably increased antibacterial effects on Propionibacterium acnés, as compared with salicylic acid (FIGS. 3A and 3B).

[0072] Experimental Example 4. Antioxidant effects of compounds of the présent disclosure

[0073] Antioxidant effects of the compounds of the présent disclosure were examined using human haïr dermal papilla cell (HHDPC), NIH3T3 fibroblast, and HaCaT kératinocyte. To this end, the three kinds of the cell lines were cultured in a 96-well plate in the same manner as in Experimental Example 2, respectively and then treated with each 50 pM of the compound 1 and the compound 3 of the présent disclosure and salicylic acid 1 hour before UV treatment. 50 mJ of UVB, 5 J of UVA, and 16 mJ of UVB were irradiated to HHDPC, NIH3T3 fibroblast, and HaCaT kératinocyte, respectively and DCFH-DA was treated thereto. The cells were incubated for 30 minutes, and then recovered. The cells were dispersed in PBS, and then FL1 values were measured using FACS, and used to examine changes in intracellular reactive oxygen species.

[0074] As a resuit, it was confirmed that the compounds of the présent disclosure did not reduce the antioxidant effect of salicylic acid used as the positive control, and in particular, the levels of intracellular reactive oxygen species generated by UV treatment were the same as or much lower than those of salicylic acid (FIGS. 4A to 6B).

[0075] Experimental Example 5. Effect of compounds of the présent disclosure on extracellular matrix expression

[0076] Effects of the compounds of the présent disclosure on extracellular matrix expression were examined using NIH3T3 fibroblast. To this end, NIH3T3 fibroblast was seeded in a 6-well plate at a density of 300,000 cells per well, and then incubated in a DMEM culture medium (Gibco, USA) containing 10% FBS for 24 hours under conditions of 37°C and 5% CO₂. The cultured cell line was detached from the bottom of the culture plate using a 1% trypsin solution, and a cell precipitate was collected by centrifugation. The cell precipitate was suspended in DMEM without FBS, and then cultured for 24 hours under conditions of 37°C and 5% CO2. 24 hours later, the medium was replaced by the same medium from which sérum was completely removed. A blank sample in which the cell precipitate was dissolved stérile in 10% DMSO was prepared for standardization. Each of the compound 1 and the compound 3 of the présent disclosure, and salicylic acid as a positive control were treated at a concentration of 50 μΜ. Incubation was carried out under the same conditions as above for 72 hours. Thereafter, to examine collagen, elastin, and fibronectin expression by immunocytostaining, cells were fixed with 4% paraformaldéhyde, permeated with 0.5% Triton X-100, and blocked with 3% BSA. The cells were treated with primary antibodies against respective collagen, elastin, and fibronectin at 1:100, and incubated at 4°C overnight. The cells were treated with secondary antibodies at 1:500, and reacted at room température for 2 hours, and then nuclear staining and mounting were carried out using DAPI, followed by observation under a fluorescent microscope.

[0077] Meanwhile, RNA was isolated from the cells treated in the same manner as the above experiment, and then cDNA was synthesized using a cDNA synthesis kit (Intron, Korea). PCR premix (Intron, Korea) and primers for each of collagen, elastin, and fibronectin in Table 2 below were used to perform PCR. Thereafter, mRNA levels of collagen, elastin, and fibronectin were determined by 5% agarose gel electrophoresis.

[0078] [Table 2]

Target	Primer sequence	SEQ ID NO
Collagen	Forward	(5’) CACCCTCAAGAGCCTGAGTC (3’)	5
Reverse	(5’) AGACGGCTGAGTAGGGAACA (3)	6
Elastin	Forward	(5’) GGACCCCTGACTCGCGACCT (3’)	7
Reverse	(5’) GGGGAGGTGGGACTGCCCAA (3 )	8
Fibronectin	Forward	(5’) CCAGGAACCGAGTACACCAT (3 )	9
Reverse	(5’) ATACCCAGGTTGGGTGATGA (3)	10

[0079] As a resuit, it was confirmed that the compound 1 and the compound 3 of the présent disclosure remarkably increased protein and mRNA expression levels of collagen, elastin, and fibronectin which constitute extracellular matrix, as compared with salicylic acid used as the positive control (FIGS. 7A and 7B).

[0080] Experimental Example 6. Anti-inflammatory effects of compounds of the présent disclosure

[0081] Anti-inflammatory effects of the compounds of the présent disclosure were examined using Propionibactehum acnés. To this end, kératinocyte was seeded in a 6-well plate at a density of 300,000 cells per well, and then incubated in a DMEM culture medium (Gibco, USA) containing 10% FBS for 24 hours under conditions of 37°C and 5% CO2. After replacing the medium by a fresh medium, the cells were treated with 50 pg/ml of Propionibacterium acnés, and then the treated Propionibactehum acnés was treated with each 50 μΜ of the compound 1 and the compound 3 of the présent disclosure and salicylic acid used as the positive control, followed by incubation for 24 hours under the same conditions as above. RNA was isolated from the cells, and cDNA was synthesized using a cDNA synthesis kit (Intron, Korea). PCR premix (Intron, Korea) and primers for each of TNF-a, IL-6, IL-1b, and COX-2 in Table 3 below were used to perform PCR. mRNA levels of TNF-a, IL-6, IL1 b and COX-2 were determined by 5% agarose gel electrophoresis.

[0082] [Table 31

Target	Primer sequence	SEQ ID NO
TNF-a	Forward	(5 ) CGTCAGCCGATTRTGCTATCT (3j	11
Reverse	(5) CGGACTCCGCAAAGTCTAAG (3 )	12
IL-6	Forward	(5) AAAGAGGCACTGCCAGAAAA (3 )	13
Reverse	(5’) ATCTGAGGTGCCCATGCTAC (3 )	14
IL-1B	Forward	(5’) TTCGACACATGGGATAACGA (3)	15
Reverse	(5’) TCTHCAACACGCAGGACAG (3 )	16
COX-2	Forward	(5 ) ATCATTCACCAGGCAAATTGC (3)	17
Reverse	(5’) GGCTTCAGCATAAAGCGI I IG (3 )	18

[0083] As a resuit, it was confirmed that the compound 1 and the compound 3 of the présent disclosure remarkably reduced expression of various inflammatory cytokines induced by Propionibacterium acnés, whereas salicylic acid used as the positive control hardly inhibited expression ofthe inflammatory cytokines (FIG. 8)

[0084] Formulation Example T. Softener

[0085] A softener including the compound of the présent disclosure prepared in Example <1-2> and consisting of the following composition was prepared according to a general method of preparing a toner.

[0086] [Table 4]

Components	Content (% by weight)
Compound of the présent disclosure	2.5
1,3-Butylene glycol	6
Glycerin	4
PEG 1500	1
Sodium hyaluronate	1
Polysorbate 20	0.5
Ethanol	8
Preservative, Pigment	Proper amount
Benzophenone-9	0.05
Fragrance	Trace amount
Purified water	Residual amount
Total	100

[0087] Formulation Example 2. Nutrient cream

[0088] A nutrient cream including the compound of the présent disclosure prepared in

Example <1-2> and consisting of the following composition was prepared according to a 10 general method of preparing a nutrient cream.

[0089] [Table 5]

Components	Content (% by weight)
Compound of the présent disclosure	2.5

Meadowfoam oil	3
Cetearyl alcohol	1.5
Stearic acid	1.5
Glyceryl stéarate	1.5
Liquid paraffin	10
Wax	2
Polysorbate 60	0.6
Sorbitan sesquiolate	2.5
Squalane	3
1,3-Butylene glycol	3
Glycerin	5
Triethanolamine	0.5
Tocopheryl acetate	0.5
Preservative, Pigment	Proper amount
Fragrance	Proper amount
Purified water	Residual amount
Total	100

[0090] Formulation Example 3. Nourishinq toner

[0091] A nourishing toner including the compound of the présent disclosure prepared in Example <1-2> and consisting ofthe following composition was prepared according to a general method of preparing a toner.

[0092] [Table 61

Components	Content (% by weight)
Compound of the présent disclosure	2.5
1,3-Butylene glycol	4
Glycerin	4

Cetearyl alcohol	0.8
Glyceryl stéarate	1
Triethanolamine	0.13
Tocopheryl acetate	0.3
Liquid paraffin	5
Squalane	3
Makadamianut oil	2
Polysorbate 60	1.5
Sorbitan sesquiolate	0.5
Carboxyvinyl polymer	1
Preservative, Pigment	Proper amount
Fragrance	Proper amount
Purified water	Residual amount
Total	100

[0093] Formulation Example 4. Essence

[0094] An essence including the compound of the présent disclosure prepared in

Example <1-2> and consisting ofthe following composition was prepared according to a 5 general method of preparing an essence.

[0095] [Table 7]

Components	Content (% by weight)
Compound of the présent disclosure	2.5
Glycerin	10
1,3-Butylene glycol	5
PEG 1500	2
Allantoin	0.1
DL-Panthenol	0.3

EDTA-2Na	0.02
Hydroxyethyl cellulose	0.1
Sodium hyaluronate	8
Carboxyvinyl polymer	0.2
Triethanolamine	0.18
Octyldodeceth-16	0.4
Ethanol	6
Fragrance, Preservative, Pigment	Proper amount
Purified water	Residual amount
Total	100

[0096] Formulation Example 5. Hair sérum

[0097] A hair sérum including the compound of the présent disclosure prepared in

Example <1-2> and consisting ofthe following composition was prepared according to a 5 general method of preparing a hair sérum.

[0098] [Table 81

Components	Content (% by weight)
Compound of the présent disclosure	1
Glycerin	10
1,3-Butylene glycol	5
PEG 1500	2
Allantoin	0.1
DL-Panthenol	0.3
EDTA-2Na	0.02
Hydroxyethyl cellulose	0.1
Sodium hyaluronate	8

Carboxyvinyl polymer	0.2
Triethanolamine	0.18
Octyldodeceth-16	0.4
Ethanol	6
Fragrance, Preservative, Pigment	Proper amount
Purified water	Residual amount
Total	100

[0099] Formulation Example 6. Hairtoner

[00100] A hair sérum including the compound of the présent disclosure prepared in

Example <1-2> and consisting ofthe following composition was prepared according to a 5 general method of preparing a hair sérum.

[00101] [Table 91

Components	Content (% by weight)
Compound of the présent disclosure	1
Glycerin	2
1,3-Butylene glycol	2
PEG 1500	2
Allantoin	0.1
DL-Panthenol	0.3
EDTA-2Na	0.02
Sodium hyaluronate	8
Carboxyvinyl polymer	0.2
Triethanolamine	0.18
Ethanol	10
Fragrance, Preservative,	Proper amount

Pigment
Purified water	Residual amount
Total	100

Claims (15)

1. A compound having a structure in which salicylic acid is linked to a peptide via a covalent bond.

2. The compound of claim 1, wherein the peptide consists of 2 to 30 amino acid sequences.

3. The compound of claim 2, wherein the peptide consists of 8 to 15 amino acid sequences.

4. The compound of claim 1, wherein the peptide is a water-soluble peptide.

5. The compound of claim 4, wherein, in the water-soluble peptide, a proportion of amino acids having hydrophilic side chains is 50% or more.

6. The compound of claim 5, wherein, in the water-soluble peptide, the proportion of amino acids having hydrophilic side chains is 70% or more.

7. The compound of claim 6, wherein, in the water-soluble peptide, the proportion of amino acids having hydrophilic side chains is 90% or more.

8. The compound of claim 5, wherein the amino acids having hydrophilic side chains are selected from the group consisting of arginine (Arg), histidine (His), lysine (Lys), aspartic acid (Asp), glutamic acid (Glu), serine (Ser), threonine (Thr), asparagine (Asn), glutamine (Gin), cysteine (Cys), selenocysteine (Sec), glycine (Gly), and proline (Pro).

9. The compound of claim 4, wherein, in the water-soluble peptide, the number of amino acids having hydrophobie side chains is 5 or less.

10. The compound of claim 9, wherein, in the water-soluble peptide, the number of amino acids having hydrophobie side chains is 3 or less.

11. The compound of claim 9, wherein the amino acids having hydrophobie side chains are selected from the group consisting of alanine (Ala), valine (Val), isoleucine (Ile), leucine (Leu), méthionine (Met), phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp).

12. The compound of claim 1, wherein the peptide is selected from the group consisting of peptides consisting of amino acid sequences of SEQ ID NO: 1 to SEQ ID NO: 4.

13. An antibacterial, anti-inflammatory, or antioxidant pharmaceutical composition comprising:

the compound of any one of daims 1 to 12.

14. An antibacterial, anti-inflammatory, or antioxidant cosmetic composition comprising:

the compound of any one of daims 1 to 12.

15. The cosmetic composition of claim 14, wherein the cosmetic composition has a formulation selected from the group consisting of a softener, a nourishing toner, a nutrient cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, a powder, a haïr tonie, a haïr cream, a haïr lotion, a haïr shampoo, a hair rinse, a hair conditioner, a haïr spray, a hair aérosol, a pomade, a sol gel, an émulsion, an oil, a wax, and an aérosol.