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NZ219496A - Pyrocatechole derivatives and their pharmaceutical compositions - Google Patents

Pyrocatechole derivatives and their pharmaceutical compositions

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Publication number
NZ219496A
NZ219496A NZ219496A NZ21949687A NZ219496A NZ 219496 A NZ219496 A NZ 219496A NZ 219496 A NZ219496 A NZ 219496A NZ 21949687 A NZ21949687 A NZ 21949687A NZ 219496 A NZ219496 A NZ 219496A
Authority
NZ
New Zealand
Prior art keywords
signifies
group
compound
methylene chloride
dihydroxy
Prior art date
Application number
NZ219496A
Inventor
Karl Bernauer
Janos Borgulya
Hans Bruderer
Prada Mose Da
Gerhard Zuercher
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of NZ219496A publication Critical patent/NZ219496A/en

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    • C07D513/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains two hetero rings
    • C07D513/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/24—Antidepressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/26—Psychostimulants, e.g. nicotine, cocaine
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C07C201/00—Preparation of esters of nitric or nitrous acid or of compounds containing nitro or nitroso groups bound to a carbon skeleton
    • C07C201/06—Preparation of nitro compounds
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    • C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
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    • C07C45/29—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by oxidation of hydroxy groups
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    • C07C45/30—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by oxidation with halogen containing compounds, e.g. hypohalogenation
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    • C07C45/27—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by oxidation
    • C07C45/30—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by oxidation with halogen containing compounds, e.g. hypohalogenation
    • C07C45/305—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by oxidation with halogen containing compounds, e.g. hypohalogenation with halogenochromate reagents, e.g. pyridinium chlorochromate
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    • C07C47/56—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings containing hydroxy groups
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    • C07C49/82—Ketones containing a keto group bound to a six-membered aromatic ring containing hydroxy groups
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    • C07C69/00—Esters of carboxylic acids; Esters of carbonic or haloformic acids
    • C07C69/017—Esters of hydroxy compounds having the esterified hydroxy group bound to a carbon atom of a six-membered aromatic ring
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    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
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    • C07D217/14—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems with radicals, substituted by hetero atoms, attached to carbon atoms of the nitrogen-containing ring other than aralkyl radicals
    • C07D217/16—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems with radicals, substituted by hetero atoms, attached to carbon atoms of the nitrogen-containing ring other than aralkyl radicals substituted by oxygen atoms
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    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/36—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems
    • C07D241/38—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems with only hydrogen or carbon atoms directly attached to the ring nitrogen atoms
    • C07D241/40—Benzopyrazines
    • C07D241/44—Benzopyrazines with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the hetero ring
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    • C07D253/02—Heterocyclic compounds containing six-membered rings having three nitrogen atoms as the only ring hetero atoms, not provided for by group C07D251/00 not condensed with other rings
    • C07D253/06—1,2,4-Triazines
    • C07D253/065—1,2,4-Triazines having three double bonds between ring members or between ring members and non-ring members
    • C07D253/07—1,2,4-Triazines having three double bonds between ring members or between ring members and non-ring members with hetero atoms, or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07D265/28—1,4-Oxazines; Hydrogenated 1,4-oxazines
    • C07D265/34—1,4-Oxazines; Hydrogenated 1,4-oxazines condensed with carbocyclic rings
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    • C07D277/20—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D277/22—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
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    • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
    • C07D277/20—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D277/32—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D277/38—Nitrogen atoms
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    • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
    • C07D277/20—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D277/32—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07D471/04—Ortho-condensed systems

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  • Pyridine Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
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Description

New Zealand Paient Spedficaiion for Paient Number £19496 2 HO DRA WIMPS Priority Date(s): . \.V. .
^V.Sfcv.
Complete Specification Filed: Class: QoCl OZJ3. Scv.QslQ?TZ7/ , Sf?./. kk?/ Publication Date: LP.tf.!???...
..J.333; P.O. Journal, No: A 6 NEW ZEALAND PATENTS ACT, 1953 No.: Date: Class Cont: ^*Q772 Class Cont:Cpf7£?ii^?. jt\.. fiJ?A /»!*< k: L H. .\£> COMPLETE SPECIFICATION 3,5-DISUBSTITUTED PYROCATECHOLE DERIVATIVES J/We, F. HOFFMANN-LA ROCHE & CO. AKTIENGESELLSCHAFT 124-184 Grenzacherstrasse, Basle, Switzerland, a Swiss Company _rr.
' ^ % ■ A hereby declare the invention for which-f-/ we pray that a patent may be granted to ?»e-/us, and the method by which it is to be performed, to be particularly described in and by the following statement: - (followed by la) O 1 o la - The catechol derivatives of the general formula HO. A V i LLp- la Rb / ^ vJ-Rc HO wherein Ra signifies nitro or cyano. Rb signifies hydrogen or halogen, Rc signifies halogen, nitro, cyano or the group -(A)n-(Q)m~R or -(A)n~Q-R2. A signifies vinylene optionally substituted by lower alkyl, n signifies the number 0 or 1. m signifies the number O or 1. R signifies 3 the group -COR . a carbocyclic aromatic group ,or an aromatic or partially unsaturated heterocyclic group attached via a carbon atom, R2 signifies hydrogen or an optionally substituted, saturated or partially 3 unsaturated, lower hydrocarbon residue, R signifies hydroxy, amino, an optionally substituted, saturated or partially unsaturated, lower hydrocarbon residue attached via an oxygen atom or an imino or lower alkylimino group or a saturated, N-containing heterocyclic group attached via a ring nitrogen atom, Q 4 signifies the group -CO- or >C=N-(Z)p-R . Z signifies an oxygen atom or an imino group, p 4 signifies the number O or 1 and R signifies hydrogen or a saturated or partially unsaturated, lower hydrocarbon residue which is optionally substituted and which is optionally attached via a carbotiyl group. the ester and ether derivatives which are hydrolyzable under physiological conditions and the pharmaceutically acceptable salts thereof possess valuable pharmacological t\'j ■ , • " ' , " ■ *1, f A,V'V _Ar< P ' 219496 properties. In particular, these compounds inhibit the enzyme catechol-O-methyltransferase (COMT). a soluble, magnesium-dependent enzyme which catalyses the transference of the methyl group of S-adenosylmethionine to a catechol substrate, whereby the corresponding methyl ethers are formed. Suitable substrates which can be O-methylated by COMT and which can thus be deactivated are. for example, extraneuronal catecholamines and exogenously-administered therapeutically active substances having a catechol structure.
The compounds of formula la above can accordingly be used in the prevention or control of illnesses in which a deactivation of extraneuronal catecholamines by COMT plays a role, for example in the prevention or control of depressions. In this case the compounds of formula la above can be used as individual compounds or in combination with other therapeutically active substances which favourably influence the course of the illness. The compounds of formula la can. however, also be used as comedications with other therapeutically active substances.
The compounds of formula la can, however, also be used to improve the prevention or control of illnesses with therapeutically active substances which have a catechol structure. The treatment of Parkinson's disease and of parkinsonism with L-dopa, a therapeutically active substance having the catechol structure, can be mentioned as an example. In such cases the compounds of formula la can be used in the form of a comedication or as combination preparations.
The field of diagnostics offers a further possibility for the use of the compounds of formula la above. After 18 the administration of [ F]-6-fluoro-L-dopa, 18 [ F]-dopamine in the brain can be visualized with the 2 aid of positron emission tomography. By adding a compound of formula la above the COMT is inhibited and thus the 18 formation of [ F]-3-0-methyldopa is prevented. In the (f-S 5 absence of a COMT-inhibitor. the [ F]-3-0-methyldopa would penetrate into the brain and lead to a greatly increased background which would make the diagnosis very much more difficult. /-^ lo Formula la above embraces not only known, but also *""* novel compounds. The compounds of formula la which are known per se fall under the general formula ?a H0\ S\ I i-Rc- H°/ ^Rb wherein Ra signifies nitro or cyano, Rb signifies 20 hydrogen or halogen. He" signifies nitro. cyano or the group -(A)n~COOH or -(A)n~Q-H, A signifies vinylene optionally substituted by lower alkyl, n signifies the number 0 or 1, Q signifies the group 4 -CO- or >C=N-(Z) -R . Z signifies an oxygen atom OP ,c or an imino group, p signifies the number 0 or 1 and 4 R signifies hydrogen or a saturated or partially unsaturated, lower hydrocarbon residue which is optionally substituted and which is optionally attached via a carbonyl group, with the proviso that 30 Ra signifies nitro when Rc" signifies cyano or nitro, 1> ester and ether derivatives which are hydrolyzable under physiological conditions and pharmaceutically acceptable salts thereof. the The novel compounds of general formula formula la are the compounds of fa hon A ' " n , lb /% v*~Rc H0 * Rb wherein Ra signifies nitro or cyano, Rb signifies hydrogen or halogen, Rc' signifies nitro, cyano or the group or - (A) q-Q-R21 , A signifies vinylene optionally substituted by lower alkyl, n signifies the number 0 or 1, ra signifies the number O or 1, R11 signifies the group -COR31, a carbocyclic aromatic grouper an aromatic or partially unsaturated heterocyclic group attached via 21 a carbon atom. R signifies an optionally substituted, saturated or partially unsaturated, lower 31 hydrocarbon residue, R signifies hydroxy, amino, an optionally substituted, saturated or partially unsaturated, lower hydrocarbon residue attached via an oxygen atom or an imino or lower alkylimino group or a saturated, N-containing heterocyclic group attached via a ring nitrogen atom, Q signifies the group 4 -CO- or >C=N-(Z)p-R , Z signifies an oxygen atom or an imino group, p signifies the number 0 or 1 and 4 R signifies hydrogen or a saturated or partially unsaturated, lower hydrocarbon residue which is optionally substituted and which is optionally attached via a carbonyl group, with the proviso that Ra signifies cyano when Rc' signifies cyano or nitro 31 and R has a significance different from hydroxy when m signifies the number O. and the ester and ether derivatives thereof which are hydrolyzable under physiological conditions and the pharmaceutically acceptable salts thereof.
Objects of the present invention are: The above I 2 1 Q * 8 # -j i c - compounds of formula la and the mentioned derivatives'/^< thereof for use as therapeutically active substances; medicaments based on these compounds and derivatives; the manufacture of such medicaments; the use of the compounds 5 and derivatives in question in the prevention or control of illnesses; the use of the compounds and derivatives in : question for the manufacture of medicaments which in a % given case inhibit the enzyme COMT in the sense of a 1 f% desired side-effect; the compounds of formula lb above and j i 10 the mentioned derivatives thereof per se; the manufacture of these compounds and derivatives; as well as intermediates for their manufacture.
| The term "lower" denotes residues and compounds with a f 15 maximum of 7. preferably a maximum of 4. carbon atoms. The | term "alkyl". taken alone or in combinations such as ? "alkyl group", "alkoxy". "alkylthio" and "alkylimino", I | denotes straight-chain or branched, saturated hydrocarbon j residues such as methyl, ethyl, propyl, isopropyl, | 20 n-butyl. s-butyl, i-butyl and t-butyl. The term "saturated | or partially unsaturated, lower hydrocarbon residue" i denotes open-chain and cyclic groups and combinations thereof. Examples of saturated and partially unsaturated lower hydrocarbon residues are: lower alkyl groups such as 25 those defined above; lower alkenyl groups such as 2-propenyl, 2-butenyl, 3-butenyl and 2-methyl-2-propenyl; C _-cycloalkyl and C. . -bicycloalkyl groups 3—/ o —10 optionally substituted by lower alkyl groups such as cyclopropyl, cyclopentyl, 2-methylcyclopentyl. cyclohexyl and 3-methylcyclohexyl; lower cycloalkenyl groups optionally substituted by lower alkyl groups such as 3-cyclopentenyl, l-methyl-3-cyclopentenyl and 3-cyclohexenyl; lower alkyl or alkenyl groups substituted by lower cycloalkyl or cycloalkenyl groups such as cyclopropylmethyl. cyclopropylethyl, cyclopentylmethyl, cyclohexylmethyl, 2-cyclohexenylmethyl and 3-cyclopropyl- O 30 „ * ? 1949 Am «3 -2-propenyl. The lower alkenyl groups preferably contain 2-4 carbon atoms; the cycloalkyl and cycloalkenyl groups preferably contain 3-6 carbon atoms.
The following come into consideration as substituents for the above lower hydrocarbon residues: Hydroxy, cyano. nitro, halogen, amino, lower alkylamino. di(lower alkyl)-amino, lower alkoxy, lower alkoxycarbonyl, aryl, 10 arylaminocarbonyl, arylcarbonyl, arylcarbonylamino, lower alkanoyloxy, lower alkanoyl, carbamoyl, mono- or di(lower alkyl)carbamoyl, lower alkylenedioxy. trifluoromethyl, carboxy, lower alkanoylamino, lower alkoxycarbonylamino and lower alkylthio. The saturated or partially 15 unsaturated, lower hydrocarbon residues are preferably unsubstituted or mono- or disubstituted.
The term "aryl" denotes carbocyclic aromatic groups, preferably mono- or bicyclic groups. Especially preferred 20 carbocyclic aromatic groups are phenyl and naphthyl groups, especially phenyl groups. These groups are optionally substitued by: halogen, trifluoromethyl, nitro, amino, mono- or di(lower alkyl)amino, lower alkyl, lower alkoxy, lower alkylthio. lower alkanoyl. lower alkoxy-25 carbonyl, carboxy. hydroxy, cyano, lower alkanoyloxy, carbamoyl, mono- or di(lower alkyl)carbamoyl, lower alkylenedioxy, lower alkanoylamino or lower alkoxycarbonylamino. The carbocyclic aromatic groups are preferably unsubstituted or mono- or disubstituted.
The term "aromatic or partially unsaturated^ heterocyclic group" preferably denotes a mono-. di- or tricyclic, aromatic or partially unsaturated, heterocyclic group with up to 5 hetero atoms from the group consisting 35 of nitrogen, sulphur and oxygen. These heterocyclic groups preferably contain 1-4 nitrogen atoms and/or an oxygen or sulphur atom. They are preferably mono- or bicyclic. The 2 1949 hecero atoms are preferably distributed on one or two rings, whereby nitrogen atoms can simultaneously also be components of 2 rings. The heterocyclic groups are preferably aromatic. They can be substituted, in this case they are preferably mono- . di- or trisubstituted. As substituents there come into consideration: halogen, trifluoromethyl. nitro, carboxy. amino, arylamino, lower alkyl. lower alkoxy. hydroxy, lower alkoxycarbonyl, lower alkanoyl. lower alkanoyloxy, oxo. lower alkylenedioxy. mercapto, lower alkylthio, lower alkylamino, di(lower alkyl)amino, c3 7~cycloalkylamino. cg 10-bicycloalkyl-amino, lower alkanoylamino. lower alkoxycarbonylamino, carbamoyl, mono- or di(lower alkyl)carbamoyl, cyano, aryl, aryl-lower alkyl, aryl-lower alkylamino, heteroaryl, heteroaryl-lower alkyl, heteroarylamino and C3 7~ -cycloalkyl. The monocyclic heterocyclic groups are preferably 5- or 6-membered and contain a maximum of four hetero atoms. The bicyclic heterocyclic groups are preferably 8- to 10-membered, with the individual rings being preferably 5- or 6-membered.
The following are to be mentioned as examples of such heterocyclic groups: Pyridyl. pyrazinyl, triazinyl, thiadiazinyl, thiazolyl. oxazolyl, oxadiazolyl, pyrazolyl, tetrazolyl, imidazolyl, thienyl, quinolinyl, isoquinolinyl, dihydroisoquinolinyl, benzoxazinyl. quinoxalinyl, benzopyranyl, benzimidazolyl, indolyl, imidazothiazolyl, imidazothiadiazolyl, imidazopyridyl, benzothiazinyl, benzoquinoxaliny1 and imidazo-benzothiazolyl.
The term "heteroaryl" denotes aromatic, heterocyclic groups as defined above.
The term "a saturated. N-containing heterocyclic group attached via a ring nitrogen atom" preferably denotes a sfiss • ,'£>.'^;"?'-r-'""* O 2194' 3- to 7-membered, preferably 4- to 6-membered, saturated N-heterocycle which, in addition to the said nitrogen atom, can contain an oxygen, sulphur or nitrogen atom as a second hetero atom. These saturated N-heterocycles can be mono- or disubstituted by: lower alkyl, hydroxy, lower alkoxy. lower alkanoyloxy. lower hydroxyalkyl. lower alkoxyalkyl, lower alkanoyloxyalkyl, lower alkoxycarbonyl, lower alkanoyl. carbamoyl, mono- or di(lower alkyl)-carbamoyl, oxo and/or lower alkylenedioxy.
The following are to be mentioned as examples of such N-containing heterocyclic groups: 4-morpholinyl. 1-pyrrolidinyl and 1-azetidinyl.
The ester and ether derivatives which are hydrolyzable under physiological conditions are preferably compounds of formula la in which at least one of the two phenolic hydroxy groups is acylated by a lower fatty acid or etherified by a lower l-alkoxycarbonyloxy-1-alkyl, lower 1-alkanoyloxy-l-alkyl or by a lower 2-oxo-i-alkyl group.
The substituent Ra preferably signifies nitro. The substituent Rb is preferably situated in the p-position to the substituent Ra and preferably signifies hydrogen, chlorine or fluorine, with hydrogen being especially preferred. The substituent Rc' preferably signifies the group -CO-R11 in which R11 signifies an aromatic mononuclear carbocyclic grouper an aromatic mononuclear O heterocyclic group,with 1-3 nitrogen atoms as the hetero ring member(s),which is attached via a carbon atom. In an especially preferred embodiment R11 signifies a phenyl group optionally mono- or disubstituted by halogen, trifluoromethyl, cyano. hydroxy or lower alkyl or a pyridyl group.
Particularly preferred compounds in the scope of the ; a* s$ ■\ Ay> V. r- 0 :: ... *..,.... ♦ 2 4 Ot A present invention are: 3,4-Dihydroxy-5-nitrobenzophenone, 2'-fluoro-3,4-dihydroxy-5-nitrobenzophenone and 3,4-dihydroxy-5-nitrophenyl 4-pyridyl ketone.
The compounds of formula lb. the ester and ether derivatives which are hydrolyzable under physiological conditions and the pharmaceutically acceptable salts thereof can be manufactured in accordance with the invention by a) cleaving off the lower alkyl ether group(s) in a compound of the general formula rc\A.
I II _ , II / V R'° • Rb wherein one of the symbols R and R1 signifies lower alkyl and the other signifies hydrogen or lower alkyl and Ra, Rb and Rc' have the above significance. or b) reacting a compound of the general formula Ra HO J \ i « lb1 HO' ^\-(A)n-CO-CH2-x Rb wherein X signifies a leaving group and Ra, Rb. A and n have the above significance. with a thioamide, thiourea, thiocarboxylic acid hydrazide, thiosemicarbazide, amidine, guanidine, amidrazone. 219496 « aminoguanidine. cyclic amidine, 1,2-diamine. 1,2-amino-thiol or a 1.2-aroinoalcohol and, if desired, dehydrogen-ating the cyclocondensation product obtained, or c) reacting a compound of the general formula fa H0S A • • lb2 O 10 y\ V*- (A)n-C°-C00R" W HO N»*u Rb wherein R" signifies lower alkyl and Ra. Rb, A and n have the above significance, with a 1,2-diamine, 1,2-aminothiol, 1,2-aminoaIcohol, semicarbazide, thiosemicarbazide. amidrazone or an aminoguanidine and. if desired, dehydrogenating the cyclocondensation product obtained, or d) reacting a compound of formula lb1 above with a fl-aminocarbonyl compound, or e) converting the carboxaldehyde group(s) in a compound of the general formula O CHO CN CHO H0N/"x. H°S HOS At- Ia2 ot AxL- IV wherein Rc"' signifies nitro, cyano or the group 12 12 31 -(A)n~R and R signifies the group -COR a carbocyclic aromatic group,or an aromatic or partially unsaturated heterocyclic group attached via a carbon atom and Ra, Rb. A. n and R3 have the above significance, or in a di-O-lower alkanoyl derivative thereof into the - ■ ; \, # V h%// * ,K \ /J r. v- C>'' 2 1 _ _ C"^TT cyano group(s). or f) reacting a di-O-lower alkanoyl derivative of a carboxylic acid of the general formula Ra fa ho HOk sK hun ^ \ i N i t jj la3 or i lb3 /\ X (A) n~C00H Ho'XV" (A,n-C°-cOOH H0 * Rb * wherein Ra, Rb. A and n have the above significance, in the presence of a condensation agent or a reactive derivative or a di-O-lower alkanoyl derivativejof a 3 3 carboxylic acid of formula la or lb with a compound of the general formula HO-R5 v or HNR6R7 VI wherein R5 signifies an optionally substituted, saturated or partially unsaturated, lower hydrocarbon residue. R6 signifies hydrogen or lower alkyl and 7 R signifies hydrogen or an optionally substituted, saturated or partially unsaturated, lower hydrocarbon 6 7 residue or R and R together with the nitrogen atom signify a saturated N-containing heterocyclic group, or 2 g) hydrolyzing a compound of formula lb or a compound of the general formula 8 R °N T i" ib' 8 /%V"RC R 0 Rb Q wherein R signifies lower alkanoyl and Ra. Rb and ! O W 30 4 e\ M. ® 2 i t -^*N ?, - 12 - Rc' have the above significance, S or ^ 5 h) reacting a compound of the general formula ?a i H°\>X /% v-"C0R i r>: 10 H0 Rb 1 | wherein Ra and Rb have the above significance and R"1 ] signifies hydrogen or lower alkyl, j or a di-O-lower alkanoyl derivative thereof in the f 15 presence of a secondary amine with a compound of the ) general formula 4 CH3CO-R23 VII 23 wherein R signifies an optionally substituted. | 20 saturated or partially unsaturated, lower hydrocarbon residue, or i) reacting a compound of the general formula ho\ A 1 ii lb* .V VJ-(A)n-C0-CH2-C00R" H0 N'\b wherein Ra, Rb, A, n and R" have the above significance. with a hydrazine or an amidine, or j) reacting a compound of formula lb above in which m signifies the number 1 and Q signifies the group -CO- with a compound of the general formula H2N-(Z)p-R4 VIII and and, if desired. 4 wherein Z, p and R have the above significance. j k) converting a compound of formula lb above into an : * ester or ether derivative which is hydrolyzable under i ] physiological conditions or into a pharmaceutically j 10 acceptable salt thereof.
I | In accordance with process variant a) the compounds of 3 formula lb can be manufactured by cleaving the ether j group(s) in a compound of formula II. This ether cleavage | 15 can be carried out according to methods which are known | per se and which are familiar to any person skilled in the i art. The ether cleavage can be carried out, for example, by treatment with hydrogen bromide in a suitable solvent. ;; Suitable solvents are, for example, water, acetic acid and i 20 mixtures thereof. The reaction is preferably carried out at an elevated temperature, for example in a temperature range of about 100°C to the boiling temperature of the - reaction mixture. There are preferably used 48 percent hydrobromic acid or mixtures thereof with acetic acid.
The ether cleavage can also be carried out by treatment with boron tribromide in a suitable solvent at temperatures of about -60°C to about room temperature. Suitable solvents are especially halogenated lower hydro-3o carbons such as methylene chloride, chloroform and the like. Further suitable methods are: treatment with pyridinium hydrochloride at temperatures of about 150°C to about 250°C and treatment with sodium iodide/silicon tetrachloride in an inert organic solvent at an elevated temperature, for example at the reflux temperature of the reaction mixture. Suitable solvents for the latter process are, for example, acetonitrile, aromatic hydrocarbons such 2 D) /-f as benzene or toluene, mixtures thereof and the like.
In accordance with process variant b) there can be © manufactured compounds of the general formula HO' N„.. ' fa \ I ii , *, /\9\~ (^n-0 hon lb7 wherein Ra. Rb. A and n have the above significance and Q1 signifies a group of the formula /Re .NHRf v. M M M N= • ,N=« ^ ^ ^ i ^ _n- ./ V-NHRf I ' I - \ / "Re ^#_,L (a), (b), N.-s' (c) , N*-s/ (d), /Re /NHRf /N»J /N=| /N=N N=N "^-L <f>' ■\,/'"Rel9)'"\v,"NHRf (h)' /N=0 /N-<R9 /N-./Rg I ?4 (1)' >"Rh (k) or -C ")-Rh (k'), •-*0' — N( -=N Rf M /Rg /R9 -*\_s/*~Rh (1) or -*( 0/'~Rh (m)' in which Re signifies hydrogen, C3 7-alkyl. c3_7~ -cycloalkyl. aryl, heteroaryl, aryl-lower alkyl or heteroaryl-lower alkyl, Rf signifies hydrogen, aryl. aryl-lower alkyl, lower alkyl, lower alkoxycarbonyl, heteroaryl. heteroaryl-lower alkyl, Cg_10-bicyclo- alkyl or C -cycloalkyl, Rg and Rh each signify 3—7 hydrogen, cyano, lower alkyl, ^-cycloalkyl, aryl, aryl-lower alkyl, heteroaryl or heteroaryl-lower alkyl 4 • .VA. .i I'i'i-'r'.-i.V Ai pv ^ • or Rg and Rh together with the two carbon atoms to which they are attached signify a carbocyclic aromatic group or an aromatic or partially unsaturated, heterocyclic group, the dotted line 4 signifies an optional bond and Q together with .the carbon atom and the nitrogen atom signify an aromatic or partially unsaturated, heterocyclic group which contains at least one nitrogen atom as a hetero ring member.
Suitable solvents for this process aspect are lower alcohols such as ethanol, n-butanol. n-hexanol and ethylene glycol, open-chain and cyclic ethers which can contain free hydroxy groups such as tetrahydrofuran. dioxan, t-butyl methyl ether, ethylene glycol dimethyl ether, diethylene glycol dimethyl ether, ethylene glycol raonomethyl ether and diethylene glycol monomethyl ether, .acetonitrile, dimethylformamide. dimethylacetamide and dimethyl sulphoxide. The desired reaction can also be carried out without a solvent by dry heating the reaction partners. The reaction is preferably carried out at an elevated temperature, for example in a range of about 50° to 150°C, whereby it is preferably carried out at the boiling temperature of the solvent insofar as it is carried out in the presence of a solvent and the boiling point lies in the previously mentioned range.
In accordance with process variant c) there can be manufactured compounds of the general formula HO.
I II -n2 HO' /% (A)n Q lb8 Rb in which Ra, Rb, A and n have the above significance # 2 and Q signifies a group of the formula >-Rh </' Rf N / Rg (n) , - /R« •s>._ \ _/ s -Rh (o) , xN-'Rg -< >-Rh </' (P) , O 10 .N-N. ,N-N^ ^N-N. ).-0H (q), -•( )--SH (r), -•( ).-Re (s) or ^*"N\ 0X H Or H 0y H c .-NHRf (t) J"N\ cr h in which Re. Rf, Rg, Rh and the dotted line have the 20 above significance.
The reaction in accordance with process variant c) can be carried out under the same reaction conditions as process variant b).
In accordance with process variant d) there can be manufactured compounds of the general formula ?a ho. j O 30 \ 1 11 _3 lb9 "V*- n~^ H0 Rb 3 wherein Q signifies the group of the formula " ^ 1 2 1949 ?f \>/Rg rv _ y—i! (u) ) 5 / s Re Rh and Ra. Rb. Re. Rf. Rg. Rh. A. n and the dotted line have the above significance. f ; 10 V..." Process variant d) can also be carried out under the same reaction conditions as process variant b).
In accordance with process variant e) there can be 15 manufactured compounds of formula lb in which Ra signifies cyano. Rc' signifies nitro. cyano or the group 12 12 31 -(A)n~R and R signifies the group -COR . a carbocyclic, aromatic group or an aromatic or partially unsaturated, heterocyclic group attached via a carbon atom 31 and Rb, A, n and R have the above significance. The conversion of the carboxaldehyde group(s) into the cyano group(s) can be effected according to methods which are known per se and which are familiar to any person skilled 2 in the art. For example, a compound of formula la , 111 25 or IV can be treated with hydroxylamine O-sulphonic acid at an elevated temperature, with water being preferably used as the solvent. The reaction can be carried out in a temperature range of about 50°C to about 100°C.
In accordance with process variant f) there can be manufactured di-O-lower alkanoyl derivatives of compounds of formula lb in which Rc1 signifies the group 32 32 -(A)n-(CO)m-COR and R signifies amino, an optionally substituted, saturated or partially 35 unsaturated, lower hydrocarbon residue attached via an oxygen atom or an imino or lower alkylimino group or a saturated, N-containing heterocyclic group attached via a " o. * 2 19496 ': - is - X ring nitrogen atom and A, n and m have the above signifi- J cance. This reaction can also be carried out according to methods which are known per se and which are familiar to 5 any person skilled in the art. Lower alkyl esters can be manufactured, for example, by treating the carboxylic acid with the corresponding lower alcohol in the presence of an acid, with the corresponding lower alcohol being preferably used as the solvent. Suitable acids are, for (3 10 example, mineral acids such as hydrogen chloride and organic sulphonic acids such as p-toluenesulphonic acid. The reaction temperature preferably lies in a range of room temperature to the boiling temperature of the chosen solvent.
The remaining esters and the amides are preferably manufactured starting from reactive carboxylic acid derivatives. Suitable reactive carboxylic acid derivatives are. for example, the corresponding carboxylic acid 20 halides, especially the carboxylic acid chlorides, corresponding carboxylic acid anhydrides and mixed anhydrides (e.g. with trifluoroacetic acid and organic sulphonic acids such as mesitylenesulphonic acid and p-toluene-sulphonic acid), corresponding carboxylic acid 25 imidazolides and the like. The reaction is conveniently carried out in the presence of an acid-binding agent and in an inert organic solvent. Suitable acid-binding agents are, for example, tertiary amines such as triethylamine and pyridine. In the manufacture of amides, excess amine v—' 30 of formula VI can also be used as the acid-binding agent.
Suitable solvents are, for example, open-chain and cyclic ethers such as tetrahydrofuran, diethyl ether, t-butyl methyl ether, dioxan, ethylene glycol, dimethyl ether or the like, halogenated hydrocarbons such as methylene 35 chloride, chloroform and 1.2-dichloroethane, acetonitrile and dimethylformamide. # ? 194 jSoo n r>, 10 M & 19 - 4 The hydrolysis o£ compounds of formula lb to the corresponding catechol derivatives in accordance with process variant g) can also be carried out according to methods which are known per se and which are familiar to any person skilled in the art. The hydrolysis can be carried out. for example, by treatment with an alkali metal hydroxide such as sodium hydroxide or potassium hydroxide in a suitable solvent. Suitable solvents are, for example, lower alcohols such methanol and water or mixtures thereof. The hydrolysis can be carried out, for example, in a temperature range of about o°C to the boiling temperature of the solvent, whereby, however, it is preferably carried out at room temperature.
In accordance with process variant h) there can be manufactured compounds of the general formula H\A.
/\\'^C(R,"1=CH-C0-R23 lb HO Rb 2 3 wherein Ra. Rb. R"' and R have the above signifi- cance, and the corresponding di-O-lower alkanoyl derivatives thereof. Cyclic amines such as pyrrolidine, piperidine. morpholine and thiomorpholine are preferably used as the secondary amine. Suitable solvents for this process are, w 30 for example, open-chain and cyclic ethers such as tetra- hydrofuran, diethyl ether, t-butyl methyl ether, dioxan, ethylene glycol and dimethyl ether, halogenated hydrocarbons such as methylene chloride, chloroform and 1.2-dichloroethane. acetonitrile and dimethylformamide.
The reaction temperature conveniently lies in a range of about 0°C to the boiling temperature of the chosen solvent whereby the reaction is preferably carried out at room f) 2 temperature. In an especially preferred embodiment the reaction is carried out in the presence of an acid, preferably a carboxylic acid such as acetic acid.
In accordance with process variant i) there can be manufactured compounds of the general formula H0\ A V"(A)n-Q5 H0 S'XRb wherein Q5 signifies the group lb" Rf /Re /N-N , J (v) or /N (W) • = • •= * v XOH 0H and Ra. Rb. Re. Rf. A and n have the above significance .
Suitable solvents for this process are, for example, lower alcohols such as methanol and ethanol, open-chain and 25 cyclic ethers such as tetrahydrofuran, diethyl ether, t-butyl methyl ether, dioxan, ethylene glycol and dimethyl ether, acetonitrile and dimethylformamide. The reaction is preferably carried out at an elevated temperature, for example in a range of about 50°C to the boiling 30 temperature of the chosen solvent, whereby it is preferably carried out at the boiling temperature of the chosen solvent.
In accordance with process variant j) there can be 35 manufactured compounds of the general formula ^ ' •** ' „ ... ... - .-*-. 2 ?a H°VX ,Ri I .11 / A \ ^ H0.-,.V-'ft,n-CV(2)p.R4 3 1 wherein Ri signifies the- group -COR , a carbocyclic, aromatic group or an aromatic or ;0 10 partially unsaturated, heterocyclic group attached via a carbon atom or an optionally substituted, saturated or partially unsaturated, lower hydrocarbon residue 31 4 and Ra. Rb, R , R , A. Z, n and p have the above significance.
This process can also be carried out according to methods which are known pet se and which are familiar to any person skilled in the art. Suitable solvents are, for example, lower alcohols such as methanol and ethanol, dimethylformamide and water. The reaction is conveniently 20 carried out at room temperature.
In accordance with process variant k) the compounds of formula lb can be converted into ester or ether ^ derivatives which are hydrolyzable under physiological conditions. Suitable ester derivatives which are hydrolyzable under physiological conditions are especially the compounds of formula lb in which at least one of the two phenolic hydroxy groups is acylated by a lower fatty acid. These can be manufactured according to methods which 30 ace known per se and which are familiar to any person skilled in the art. In a preferred embodiment the acylation is carried out with the corresponding lower fatty acid anhydride in the presence of a catalytic amount of a strong acid, with excess fatty acid anhydride being 35 preferably used as the solvent. Suitable acids are. for example, sulphuric acid and organic sulphonic acids such as p-toluenesulphonic acid.
■ ' -:.W". . ,_.v.„ . a f\ m 2 Suitable ether derivatives which are hydrolyzable under physiological conditions are, for example, compounds of formula lb in which at least one of the two phenolic 5 hydroxy groups is etherified by a lower 1-alkoxycarbonyl-oxy-l-alkyl, lower 1-alkanoyloxy-l-alkyl or by a lower 2-oxo-l-alkyl group. The ether ification can be carried out according to methods which are known per se and which are familiar to any person skilled in the art. For example, a (">; 10 compound of formula lb can be reacted with a lower 1-alkoxycarbonyloxy-l-alkyl halide. a lower 1-alkanoyloxy--1-alkyl halide or a lower 2-oxo-l-alkyl halide, with this etherification being conveniently carried out in the presence of a base. As halides there come into considera-15 tion, in particular, the iodides. Suitable bases are, for example, alkali metal hydroxides and alkali metal carbonates such as sodium hydroxide and sodium carbonate.
C In accordance with process variant k) compounds of 20 formula lb above can also be converted into pharmaceutically acceptable salts. As salts there come into consideration, in particular, salts with pharmaceutically acceptable bases. As examples of such salts there are to be mentioned the alkali metal salts such as the sodium and 25 potassium salts. These salts can be manufactured according to methods which are known per se and which are familiar to any person skilled in the art.
The various compounds which are used as starting a 30 materials are known or can be prepared according to methods known per se. The following Examples contain detailed information concerning the preparation of these starting materials.
As mentioned earlier, the compounds of formula la inhibit the enzyme COMT. This activity can be determined quantitatively as follows: Rat liver homogenate is ....-rf..../ •. , ,. jS^ w V 2 1949 ri incubated in the presence of a suitable substrate as described in J. Neurochem. 38., 191-195 (1982) and the COMT activity is measured. In a second series of experiments the incubation is carried out in the presence of a compound of formula la. The IC_. can then be calculated 50 from the difference of the COMT activity which is determined. is given in nmol/1 and is that concen tration in the incubation mixture which is required to reduce the COMT activity by 50%. The IC values for 50 some compounds of formula la are given in the following Table. Moreover, this Table contains data concerning the acute toxicity of these compounds (LD in mg/kg in the 50 case of single oral administration to mice).
Compound of formula la IC in nmol/1 50 LD50 in mg/kg p.o 3.4-Dihydroxy-5-nitrophenyl 2-pyridyl ketone 3,4-Dihydroxy-5-nitrophenyl 3-pyridyl ketone 3.4-Dihydroxy-5-nitrophenyl 4-pyridyl ketone n-Butyl 3,4-dihydroxy-5--nitrobenzoate n-Butyl 3,4-dihydroxy-5--nitrocinnamate Ethyl 3,4-dihydroxy-5--nitrophenylglyoxylate 3.4-Dihydroxy-5-nitrobenzo-phenone 3.5-Dinitropyrocatechol 21-Fluoro-3.4-dihydroxy-5-nitrobenzophenone 53 .4 47 .0 67.0 .0 .9 48 .1 48 36 0 9 42.0 1250-2500 1000-2000 1000-2000 312- 625 2500-5000 1250-2500 500-1000 500-1000 312- 625 The described substances can be used as medicaments, e.g. in the form of pharmaceutical preparations for 2 1949 enteral or parenteral administration. The compounds of formula la can be administered, for example, perorally, e.g. in the form of tablets, coated tablets, dragees, hard 5 and soft gelatine capsules, solutions, emulsions or suspensions, rectally, e.g. in the form of suppositories, or parenterally, e.g. in the form of injection solutions.
The manufacture of the pharmaceutical preparations can y'-v be effected in a manner which is familiar to any person skilled in the art by bringing the compounds of formula la, optionally in combination with other therapeutically valuable substances, into a galenical administration form together with suitable, non-toxic, inert, therapeutically 15 compatible solid or liquid carrier materials and, if desired, the usual pharmaceutical adjuvants.
As carrier materials there are suitable not only inorganic carrier materials, but also organic carrier 20 materials. Thus, for tablets, coated tablets, dragees and hard gelatine capsules there can be used as carrier materials, for example, lactose, maize starch or derivatives thereof, talc, stearic acid or its salts. Suitable carriers for soft gelatine capsules are. for 25 example, vegetable oils, waxes, fats and semi-solid and liquid polyols (depending on the nature of the active substances no carriers are, however, required in the case of soft gelatine capsules). Suitable carrier materials for the manufacture of solutions and syrups are, for example, 30 water, polyols, saccharose, invert sugar and glucose.
Suitable carrier materials for injection solutions are. for example, water, alcohols, polyols, glycerine and vegetable oils. Suitable carrier materials for suppositories are, for example natural or hardened oils, waxes. 35 fats and semi-liquid or liquid polyols.
As pharmaceutical adjuvants there come into considera- % *?1 ic ~v i O 2 194-96 tion the usual preserving agents, solubilizers, stabilizing agents, wetting agents, emulsifying agents, -Tr flavour-improving agents such as sweetening agents and 5 flavouring agents, colouring agents, salts for varying the osmotic pressure, buffers, coating agents and antioxidants.
The dosage of the described substances can vary within wide limits depending on the illness to be treated, the age and the individual condition of the patient and on the mode of administration and will, of course, be fitted to the individual requirements in each particular case. In the improvement of the treatment of Parkinson's disease and of parkinsonism with L-dopa a daily dosage of 25 mg to 15 about 1000 mg, especially about 100 rag to about 300 mg. | comes into consideration. Depending on the dosage it is ! convenient to administer the daily dosage in several i dosage units.
; ! The pharmaceutical preparations in accordance with the invention conveniently contain about 25 mg to about 300 mg. preferably about 50 mg to about 150 mg. of a i i compound of formula la or of an ester or ether derivative $ ] which is hydrolyzable under physiological conditions or of • N~' 25 a pharmaceutically acceptable salt thereof.
The following Examples are intended to illustrate the present invention in more detail, but are not intended to limit its scope in any manner. All temperatures are given 30 in degrees Celsius.
Example 1 a) 17.1 g (86.7 mmol) of 4-hydroxy-3-methoxy-5-nitro-35 benzaldehyde are treated with 170 ml of constant-boiling hydrobromic acid and heated under reflux for 3.5 hours. After cooling the separated precipitate is filtered off 2 19496 under suction, washed twice with ice-water and taken up in ethyl acetate. The organic phase is washed twice with 50 ml of sodium chloride solution each time, dried over 5 magnesium sulphate and evaporated in a water-jet vacuum. The crystals obtained are taken up in methylene chloride, whereupon the solution is filtered over a ten-fold amount of silica gel. The material obtained is crystallized from ethyl acetate/isopropyl ether. There is obtained 10 3,4-dihydroxy-5-nitrobenzaldehyde in the form of yellow crystals of m.p. 142-143°. b) A solution of 1.7 g (15 mmol) of hydroxylamine O--sulphonic acid in 6 ml of water is added to a solution of 15 1.83 g (10 mmol) of 3.4-dihydroxy-5-nitrobenzaldehyde in 25 ml of water, subsequently stirred at 65° for 3.5 hours, cooled, the separated precipitate is filtered off under suction and taken up in ethyl acetate. The organic phase is dried over sodium sulphate and evaporated in a water-20 -jet vacuum. The crystals obtained are recrystallized from ethyl acetate/n-hexane. There is obtained 3,4-dihydroxy-5--nitrobenzonitrile in the form of yellow crystals of m.p. 194-195°.
O Example 2 aa) 10 ml of tert.butyl lithium solution (1.4M in hexane) are added dropwise at -70° within 10 min. to 4.1 g of 4 - (benzyloxy) -3- methoxy-bromobenzene dissolved in 40 ml of ( "5 v*-"' 30 tetrahydrofuran. After stirring at -70° for 2 hrs. 1 ml of pyridine-3-carbaldehyde is added within 5 min. The reaction mixture is stirred at -70° for 1 hr. and at 0° for 2 hrs. and poured into 100 ml of IN hydrochloric acid. The mixture is extracted three times with 50 ml of ether 35 each time. The combined ether phases are washed with 100 ml of IN hydrochloric acid and 20 ml of water. The combined aqueous phases are made alkaline with aqueous y-':.-/ -> v.-" - ,7>4; #1 2 19496 ammonia solution and extracted three times with 100 ml of methylene chloride each time. The combined methylene chloride phases are dried over sodium sulphate and c 5 evaporated. There is obtained alpha-[4-(benzyloxy)-3-methoxyphenyl]-3- -pyridinemethanol as an oil. ab) In an analogous manner, using pyridine-4-carbaldehyde 10 there is obtained alpha-[4-(benzyloxy)-3-methoxyphenyl]-4--pyridinemethanol as an oil. ba) 3.2 g of alpha-[4-(benzyloxy)-5-methoxyphenyl]-3--pyridinemethanol suspended in 50 ml of water are treated 15 with 2.5 g of potassium permanganate, whereupon the mixture is stirred at 90° for 30 min. After adding a further 1.0 g of potassium permanganate and stirring for a further 30 min. at 90° the mixture is cooled to room temperature and extracted twice with 150 ml of ethyl 20 acetate each time. The combined ethyl acetate phases are washed with sodium chloride solution, dried over sodium sulphate and evaporated. The thus-obtained residue is chromatographed on 50 g of silica gel with ethyl acetate. After recrystallization from methylene chloride/hexane 25 there is obtained 4-(benzyloxy)-3-methoxyphenyl 3-pyridyl ketone of m.p. 76°. bb) In an analogous manner, from alpha-[4-(benzyloxy)-3--methoxypheny1)-4-pyridinemethanol there is obtained 30 4-(benzyloxy)-3-methoxyphenyl 4-pyridyl ketone of m.p. 85-87° (methylene chloride/hexane). ca) 50 ml of 33 percent hydrobromic acid in acetic acid are added dropwise within 15 min. at 10° to 20 g of 35 4-(benzyloxy)-3-methoxyphenyl 3-pyridyl ketone dissolved in 200 ml of methylene chloride. After stirring at 20° for 3 hrs. the reaction mixture is poured into a mixture of mm®-.? ,v" 2 19496 100 ml of conc. aqueous ammonia and ice. The pH is adjusted to 6 by adding acetic acid. The methylene chloride phase is separated; the aqueous phase is 5 extracted a further twice with 100 ml of methylene chloride each time. The combined methylene chloride phases are dried over sodium sulphate and evaporated. The residue is recrystallized from methylene chloride/hexane. There is obtained 4-hydroxy-3-methoxyphenyl 3-pyridyl ketone of v,;.. 10 m.p. 150-151°. cb) In an analogous manner, from 4-(benzyloxy)-3-methoxy-phenyl 4-pyridyl ketone there is obtained 4-hydroxy-3--methoxyphenyl 4-pyridyl ketone of m.p. 215-218° 15 (acetonitrile). da) 0.38 ml of 65 percent nitric acid is added dropwise at room temperature to 1.15 g of 4-hydroxy-3-methoxyphenyl 3-pyridyl ketone dissolved in 15 ml of acetic acid. After stirring for 2 hrs. the reaction mixture is poured into 120 ml of ice-water, whereupon the mixture is adjusted to pH 5 with conc. ammonia and the precipitate formed is filtered off. The thus-obtained residue is heated under reflux in 20 ml of acetonitrile. whereupon it is again ^ 25 filtered off. There is obtained 4-hydroxy-3-methoxy-5- -nitrophenyl 3-pyridyl ketone as brown crystals of m.p. 193°. db) In an analogous manner, from 4-hydroxy-3-methoxyphenyl w 30 4-pyridyl ketone there is obtained 4-hydroxy-3-methoxy-5- -nitrophenyl 4-pyridyl ketone of m.p. 240°. e) 3.5 g of 4-hydroxy-3-methoxy-S-nitrophenyl 3-pycidyl ketone dissolved in 70 ml of 48 percent aqueous hydro-35 bromic acid are stirred at 100° for 18 hrs. The reaction mixture is subsequently evaporated under reduced pressure. The residue is recrystallized from water. There is 2 19496 obtained 3,4-dihydroxy-5-nitrophenyl 3-pyridyl ketone hydrobromide of m.p. 265°. f) In an analogous manner, from 4-hydroxy-3-methoxy-5--nitrophenyl 4-pyridyl ketone there is obtained 3,4-dihydroxy-5-nitrophenyl 4-pyridyl ketone of m.p. 246° (from water). g) 13.2 g of 3,4-dihydroxy-5-nitrophenyl 4-pyridyl ketone are suspended in 500 ml of methanol and treated while stirring with 4.88 g of methanesulphonic acid. The suspension is heated under reflux for 60 minutes. It is subsequently cooled to 10°. the crystals are filtered off under suction and washed twice with 30 ml of methanol each time. There is obtained 3,4-dihydroxy-5-nitrophenyl 4-pyridyl ketone methanesulphonate of m.p. 260-261° (dec.).
Example 3 a) A solution of 2.6 g (12.2 mmol) of 4-hydroxy-3--methoxy-5-nitrobenzoic acid in 26 ml of constant-boiling hydrobromic acid is heated under reflux for 2 hours. After cooling the solvent is distilled off in a water-jet vacuum. The crystalline residue is recrystallized from 50 ml of water at boiling temperature. There is obtained 3.4-dihydroxy-5-nitrobenzoic acid in the form of yellow crystals of m.p. 224-226°. b) 1.0 g (5 mmol) of 3,4-dihydroxy-5-nitrobenzoic acid is treated with 20 ml of methanolic hydrochloric acid, stirred at 45° for 3 hours and. after removing the solvent, the residue is taken up in methylene chloride. The organic phase is washed with sodium chloride solution, dried over sodium sulphate and evaporated. The crystalline product obtained is taken up in methylene chloride and f 'Kitof-J-i -' ' jA'C'.1 ^,~:.;x.,„s | ""V filtered over a ten-fold amount of silica gel. The material obtained is recrystallized from ethyl acetate/n- -hexane. There is obtained methyl 3,4-dihydroxy-5-nitro- benzoate in the form of yellow crystals of m.p. 144-145°. w»»* The following esters are obtained in an analogous manner starting from 3.4-dihydroxy-5-nitrobenzoic acid: c) Ethyl 3.4-dihydroxy-5-nitrobenzoate of m.p. 106-107° (from ethyl acetate/n-hexane), d) n-butyl 3.4-dihydroxy-5-nitrobenzoate of m.p. 73-74° (from methylene chloride) and e) n-hexyl 3,4-dihydroxy-5-nitrobenzoate of m.p. 44-45° (from isopropyl ether).
Example 4 a) 25 ml of 2M phenyl lithium solution (in benzene/ether (7:3)) are added dropwise within 15 min. to 10.0 g of 3,4-dimethoxy-5-nitrobenzaldehyde dissolved in 150 ml of tetrahydrofuran and the mixture is stirred at 0° for 1 hr. and at 20° for 2 hrs. The mixture is subsequently treated with 150 ml of 2N sulphuric acid and extracted three times with 150 ml of ether. The combined ether phases are washed with sodium chloride solution, dried over sodium sulphate and evaporated. The thus-obtained residue is chromato-graphed on 180 g of silica gel with methylene chloride. There is obtained 3,4-dimethoxy-5-nitrobenzhydrol as an amorphous solid. b) 2.5 g of 3.4-dimethoxy-5-nitrobenzhydrol dissolved in bO ml of methylene chloride are treated with 2.2 g of pyridinium chlorochromate. wheceupon the mixture is stirred at room temperature for 2 hrs. The insoluble constituents are subsequently filtered off. The filtrate is evaporated and the residue is chromatographed on 60 g of silica gel with methylene chloride. After crystalliza- 2 ■ A I ^ ^' - 31 tion from methylene chloride/hexane there is obtained 3.4-dimethoxy-5-nitrobenzophenone of m.p. 78-80°. c) 0.5 g of 3,4-d imethoxy-5-ni t robenzophenone is stirred at 110° for 30 hrs. in a mixture of 4 ml of acetic acid and 4 ml of 48 percent aqueous hydrobromic acid. The reaction mixture is subsequently evaporated to dryness. The residue is taken up in methylene chloride. It is washed with water, dried over sodium sulphate and evaporated. After recrystallization from methylene chloride/ hexane there is obtained 3.4-dihydroxy-5-nitrobenzophenone of m.p. 132°.
Example 5 a) 4.9 g (0.2 mol) of magnesium are suspended in 15 ml of absolute ethanol and. after adding 1 ml of carbon tetrachloride. warmed until the reaction starts. A solution of 31.8 g (0.2 mol) of diethyl malonate in 19.9 ml of absolute ethanol and 80 ml of absolute toluene is then added dropwise while stirring so that the temperature lies between 50° and 60°. The reaction mixture is subsequently stirred at this temperature for a further 1 hour. whereupon it is cooled to -5° and a solution of 49.3 g (0.2 mol) of 3.4-dimethoxy-5-nitrobenzoyl chloride (m.p. 82-85°) in 300 ml of absolute toluene and 50 ml of absolute tetrahydrofuran is added dropwise so that the temperature does not exceed -5°. The mixture is subsequently stirred at room temperature overnight. After distillation of the solvent the residue is dissolved in 500 ml of ethyl acetate. The solution is treated while stirring and cooling with ice with an ice-cold solution of 12 ml of concentrated sulphuric acid in 80 ml of water. The organic phase is washed with sodium chloride solution, dried over magnesium sulphate and evaporated. The oil obtained is chromatographed on a ten-fold amount of silica a'.'-:-'-'-.'j.- II i Q A 2 J 9 4 9 6 gel with methylene chloride. The crystalline material obtained is recrystallized from isopropyl ether. There is obtained diethyl 3,4-diraethoxy-5-nitrobenzoylmalonate in 5 the form of pale beige crystals of m.p. 70°. b) 19.0 g (51.4 mmol) of diethyl 3.4-dimethoxy-5-nitro-benzoylmalonate are dissolved in 100 ml of glacial acetic acid. 5 drops of concentrated sulphuric acid are added thereto and the mixture is heated under reflux for 16 hours. The acetic acid is distilled off at 60° in a water--jet vacuum and the residue is treated three times with 250 ml of toluene each time, whereby it is evaporated each time. The crystalline residue is extracted with ethyl 15 acetate. The organic phase is washed with water, dried over magnesium sulphate and evaporated. The crystals obtained are chromatographed on a 30-fold amount of silica gel with toluene. The crystalline material obtained is recrystallized from isopropyl ether. There is obtained 20 3.4-dimethoxy-5'-nitroacetophenone in the form of yellowish crystals of m.p. 86-87°. c) 2 g (8.9 mmol) of 341-dimethoxy-51-nitroacetophenone Care treated with 30 ml of constant-boiling hydrobromic ' 25 acid and stirred at 140° for 2.5 hours. After cooling the mixture is poured into 200 ml of ice-water and extracted three times with 100 ml of ethyl acetate each time. The organic phase is washed twice with 25 ml of sodium chloride solution each time, dried over sodium sulphate 30 and evaporated. The product obtained is filtered with ethyl acetate over a 20-fold amount of silica gel. By recrystallization of the material obtained from water there is obtained 3',4'-dihydroxy-5'-nitroacetophenone in the form of yellow crystals of m.p. 159-160°.
The same compound is obtained starting from 4'-hydroxy-3'-methoxy-5'-nitroacetophenone by treatment .. Jl. ■ - - _0.; . - 2 194 - 33 - - — with hydrobromic acid at the boiling temperature.
Example 6 a) 100 g (0.8 mol) of guaiacol are dissolved in 136.4 g (0.86 mol) of isobutyric anhydride, treated with 120 g (0.88 mol) of anhydrous zinc chloride (whereby all passes into solution), the reaction mixture is heated to 155° and cooled after three minutes. The residue is firstly subjected to a steam distillation in order to remove readily volatile constituents and is then extracted three times with 500 ml of ether each time. The organic phase is washed twice with 250 ml of water each time, once with 15 150 ml of saturated bicarbonate solution and again with 250 ml of water, dried over sodium sulphate and evaporated in a water-jet vacuum. The brown resin obtained is distilled in a high vacuum. The distillate of b.p. 105-120° (6.67 Pa) is dissolved in ether, whereupon the 20 solution is treated with n-hexane until crystallization begins. The crystals obtained are recrystallized from ether/hexane. There is obtained 4•-hydroxy-3'-methoxy-2--methyl-propiophenone in the form of colourless crystals of m.p. 86-87°. b) 15.0 g (77.2 mmol) of 4'-hydroxy-3'-methoxy-2-methyl--propiophenone are dissolved in 300 ml of glacial acetic acid and 7.65 ml of 50.5 percent nitric acid (11.2N) in 40 ml of glacial acetic acid are added dropwise thereto while stiring within 15 minutes. After 15 minutes the reaction mixture is poured into ice-water and the separated crystals are filtered off under suction, washed with water and dissolved in methylene chloride. The solution is dried over sodium sulphate and evaporated. The 35 crude product obtained is taken up in methylene chloride and filtered over 100 g of silica gel. The thus-obtained crystals are recrystallized from methylene chloride/ n-hexane. There is obtained 4•-hydroxy-3'--methyl-5'-nitropropiophenone in the form crystals of m.p. 85-87°. methoxy-2-of yellow c) 8.0 g (33.4 mmol) of 4'-hydroxy-31-methoxy-2-methyl--5'-nitropropiophenone are treated with 64 g of pyridine hydrochloride and stirred at 180° for 45 minutes. After cooling the reaction mixture is poured into 500 ml of ice-water, whereupon it is made acid with 20 ml of 3N hydrochloric acid and extracted with methylene chloride. The organic phase is washed with water, dried over sodium sulphate and evaporated in a water-jet vacuum. After crystallization from methylene chloride/n-hexane there is obtained 31,4'-dihydroxy-2-methyl-5'-nitropropiophenone in the form of yellow crystals of m.p. 98-99°.
Example 7 a) 100 g (805.5 mmol) of guaiacol are dissolved in 136.4 g (86.2 mmol) of butyric anhydride, treated with 120 g (880 mmol) of zinc chloride, heated for 3 minutes as given in Example 6.a and then worked-up as described there. The crude product obtained after high vacuum distillation is chromatographed with toluene on 600 g of silica gel. After recrystallization from ether/n-hexane there is obtained 4 *-hydroxy-3'-methoxy-butyrophenone in the form of colourless crystals of m.p. 40-41°. b) 6.5 ml of 11.2N nitric acid are added dropwise to a solution of 12.7 g (65.4 mmol) of 4'-hydroxy-31-methoxy-butyrophenone in 250 ml of glacial acetic acid while stirring within 10 minutes. The reaction mixture is subsequently stirred for 15 minutes, poured into ice--water. the separated precipitate is filtered off under suction, washed with ice-water and taken up in methylene chloride. The methylene chloride solution is filtered over ^--■sSfcS ••-•• •:■■ "''Sw #S/v»i,v.» ^VU+*" 1 2 194 50 g of silica gel. The material obtained is recrystallized from methylene chloride/n-hexane. There is obtained 4'-hydroxy-3'-methoxy-5'-nitrobutyrophenone in the form of yellow crystals of m.p. 92-93°. c) 10.2 g (42.6 mmol) of 41-hydroxy-31-methoxy-5'-nitrobutyrophenone are treated with 80 g of pyridine hydrochloride and stirred at 200° for 40 minutes. After cooling /"7s 1° the reaction mixture is poured into 500 ml of ice-water.
The mixture is treated with 30 ml of 3N hydrochloric acid and extracted with methylene chloride. The organic phase is dried over sodium sulphate and evaporated. The crude product obtained is chromatographed with methylene chloride on 150 g of silica gel. The material obtained is recrystallized from methylene chloride/n-hexane. There is obtained 3' ,4'-dihydroxy-5'-nitrobutyrophenone in the form of yellow crystals of m.p. 88-90°.
Example 8 a) 2.25 g (10 mmol) of 3.4-dihydroxy-5-nitrocinnamic acid are dissolved in 50 ml of methanol and hydrochloric acid gas is introduced into this solution for 10 minutes. After f 1 hour 50 ml of isopropyl ether are added thereto, and the separated precipitate is filtered off under suction and washed with isopropyl ether. After recrystallization from methanol/ether there is obtained methyl 3.4-dihydroxy-5--nitrocinnamate in the form of yellow crystals of m.p. 186-187°. b) In an analogous manner, from 3.4-dihydroxy-5-nitro-cinnamic acid and butanolic hydrochloric acid solution there is obtained n-butyl 3,4-dihydroxy-5-nitrocinnamate in the form of yellowish crystals of m.p. 129-130°. ■ "?; ' ft 2 - 36 -Example 9 .0 g (13.5 mmol) of diethyl 3,4-dimethoxy-5-nitro-O 5 benzoyl malonate are dissolved in 50 ml of absolute methylene chloride. After cooling to -20° a solution of 16.9 g (67.5 mmol) of boron tribromide in 30 ml of methylene chloride is added dropwise thereto while stirring so that the temperature does not exceed -20°. The 10 mixture is subsequently stirred at room temperature overnight. After adding 80 ml of ethanol the mixture is stirred at room temperature for 30 minutes and the solvent is subsequently distilled off in a water-jet vacuum. The residue is treated with water and extracted with methylene 15 chloride. The organic phase is washed with water, dried over sodium sulphate and evaporated. The crude product obtained is filtered with ethyl acetate over 50 g of silica gel. The crystalline residue obtained is recrystallized from methylene chloride/n-hexane. There is 20 obtained ethyl 3,4-dihydroxy-5-nitro-benzoylacetate in the form of yellow crystals of m.p. 141-142°.
Example 10 y— a) A solution of 1.49 g (12.3 mmol) of 2-phenylethylamine in 100 ml of methylene chloride is treated with 1.26 g of triethylamine. A solution of 3.0 g of 3.4-dimethoxy-5--nitrobenzoyl chloride in 100 ml of methylene chloride is added dropwise thereto while stirring, whereupon the 30 mixture is stirred for a further 15 minutes. The organic phase is then extracted twice with 50 ml of ice-water each time, dried over sodium sulphate and evaporated in a water-jet vacuum. After recrystallization from methylene chloride/n-hexane there is obtained 3,4-dimethoxy-5-nitro-35 -N-phenethylbenzamide in the form of pale beige needles of m.p. 121-122°. # b) 3.6 g (10.9 mmol) of 3,4-dimethoxy-5-nitro-N--phenethylbenzamide are heated under reflux with 36 ml of phosphorus oxychloride under a nitrogen atmosphere for 96 hours. After distilling off the excess phosphorus oxychloride in a water-jet vacuum at 60° the residue is treated three times with 100 ml of toluene each time, with the solvent* being distilled off each time. The residue is taken up in methylene chloride. The organic phase is 10 washed with water, dried over sodium sulphate and evaporated in a water-jet vacuum. The red resin obtained is chromatographed on 120 g of silica gel with methylene chloride/ethyl acetate (1:1). There is obtained l-(3,4--dimethoxy-5-nitrophenyl)-3.4-dihydroisoquinoline in the 15 form of a yellow resin. c) 1.4 g (4.5 mmol) of l-(3,4-dimethoxy-5-nitrophenyl)--3.4-dihydroisoquinoline are treated with 15 ml of constant-boiling hydrobromic acid and heated to boiling under reflux under a nitrogen atmosphere for 1.5 hours.
After distilling off the hydrobromic acid in a water-jet vacuum the crystalline residue is recrystallized from acetone. There is obtained 5-(3,4-dihydro-l--isoquinolinyl)-3-nitropyrocatechol hydrobromide in the 25 f orm of yellow crystals of m.p. >250° (decomposition).
Example 11 a) 5.0 g (27.7 mmol) of 4-hydroxy-5-methoxy-isophthal-O 30 aldehyde are treated with 50 ml of constant-boiling hydrobromic acid and heated to boiling under reflux and while stirring under an argon atmosphere for 3 hours.
After cooling 50 ml of ice-water are added thereto, and the separated precipitate is filtered off under suction 35 and washed with water. The crude product is taken up in ethyl acetate and filtered over 50 g of aluminium oxide (activity grade 11). The crystalline material obtained is m 2 4 recrystallized from ethyl acetate/n-hexane. There is obtained 4,5-dihydroxyisophthalaldehyde in the form of slightly orange crystals of m.p. 201-202°. b) A solution of 3.27 g (28.9 mmol) of hydroxylamine O--sulphonic acid in 12 ml of water is added dropwise at 30c while stirring to a solution of 2.0 g (12.0 mmol) of 4,5-dihydroxyisophthalaldehyde in 20 ml of water. 10 whereupon the mixture is held at 65° for 10 hours. After cooling the separated precipitate is filtered off under suction, washed with water and taken up in ethyl acetate. The organic phase is washed with water, dried over sodium sulphate and evaporated in a water-jet vacuum. After 15 recrystallization from ethyl acetate there is obtained 4,5-dihydroxyisophthalonitrile in the form of yellow crystals which decompose above 300°.
Example 12 a) A solution of 38 g of fuming nitric acid (96%) in 50 ml of glacial acetic acid is added dropwise while stirring and within 30 minutes at 20-25° to a solution of 112.5 g of 2-bromo-4'-hydroxy-31-methoxyacetophenone in 25 560 of glacial acetic acid. Yellow-brown crystals thereby separate. After 90 minutes the reaction mixture is poured on to 300 g of ice. The crystals are filtered off under suction, washed with 1000 ml of water and dissolved in 1000 ml of methylene chloride. The organic phase is 30 washed with saturated sodium chloride solution, dried over sodium sulphate, filtered and the filtrate is evaporated at 50° in a water-jet vacuum until crystallization begins. The crystallizate, cooled to room temperature, is filtered off under suction and washed with a small amount of 35 methylene chloride. There is obtained 2-bromo-41-hydroxy--3'-methoxy-51-nitroacetophenone of m.p. 147-149°. m 2 1949$ b) Method A: ba) A suspension of 580.1 mg of 2-bromo-4'-hydroxy-31-(**) 5 -methoxy-51-nitroacetophenone in 10 ml of ethanol is treated with 443.8 rag of selenium dioxide and heated under reflux for 71 hours. Thereafter, the selenium is filtered off and the filtrate is evaporated. The residue is dissolved in methylene chloride, washed with saturated 10 sodium chloride solution, dried over sodium sulphate, filtered and evaporated. There is obtained ethyl 4-hydroxy-3-methoxy-5-nitrophenylglyoxylate of m.p. 165-167° (from ethanol).
In an analogous manner: bb) From 2-bromo-4•-hydroxy-3'-methoxy-51-nitroacetophenone and n-butanol there is obtained n-butyl 4-hydroxy--3-methoxy-5-nitrophenylglyoxylate of m.p. 105-107° (from ethanol) and be) from 2-bromo-4'-hydroxy-31-methoxy-5'-nitroacetophenone and n-hexanol there is obtained hexyl 4-hydroxy-3--methoxy-5-nitrophenylglyoxylate of m.p. 103-105° (from 25 n-hexanol/petroleum ether). c) Method B: ca) A suspension of 29.01 g of 2-bromo-4'-hydroxy-3'-30 -methoxy-5'-nitroacetophenone in 300 ml of tert.butanol is treated with 27.74 g of selenium dioxide and heated to boiling under reflux for 18 hours. The hot reaction mixture is suction filtered through a filter aid of diatomaceous earth while rinsing with methylene chloride. 35 The filtrate is evaporated and the residue is suspended in 150 ml of hot methylene chloride. The crystalline precipitate is filtered off under suction and washed with "f '7 2 194 9 r> a small amount of methylene chloride. There is obtained 4-hydroxy-3-methoxy-5-nitrophenylglyoxylic acid of m.p. 169-171°. 2.42 g of 4-hydroxy-3-methoxy-5-nitrophenylglyoxylic acid are dissolved in 25 ml of dry N,N-dimechylformamide, treated at room temperature with 50 rag of 4-dimethylamino-pyridine and 920 rag of dry methanol, subsequently cooled 10 to 0° with an ice-bath and 2.27 g of N.N-dicyclohexyl-carbodiimide are added thereto. After 10 minutes the ice-bath is removed and the reaction mixture is stirred for a further one hour at room temperature. The mixture is subsequently evaporated. The residue is dissolved in ethyl 15 acetate, whereupon insoluble urea is filtered off, the filtrate is washed four times with water, dried over sodium sulphate, filtered and evaporated. There is obtained methyl 4-hydroxy-3-methoxy-5-nitrophenyl-glyoxylate of m.p. 155-157° (from methylene chloride/ 20 ether).
In an analogous manner: cb) From 4-hydroxy-3-methoxy-5-nitrophenylglyoxylic acid 25 and ethanol there is obtained ethyl 4-hydroxy-3-methoxy-5--nitrophenylglyoxlate of m.p. 165-167° (from ethanol) and c cc) From 4-hydroxy-3-methoxy-5-nitrophenylglyoxylie acid and isopropanol there is obtained i-propyl 4-hydroxy-3--methoxy-5-nitrophenylglyoxylate of m.p. 99-101° (from isopropanol). d) A suspension of 17.2 g of ethyl 4-hydroxy-3-methoxy-5--nitrophenylglyoxylate in 100 ml of dry acetonitrile and 35 100 ml of dry toluene is treated with 10.53 g of sodium iodide and 11.9 g of silicon tetrachloride and heated under reflux for 47 hours. The reaction mixture is then - 41 2 194 evaporated and the residue is distilled six times with 200 ml of toluene each time. The residue obtained is partitioned between water and ether and filtered through a ^ 5 filter aid of diatomaceous earth. The ethereal phase is washed with four times with saturated sodium chloride solution, dried over sodium sulphate, filtered and evaporated. The oily residue is treated three times with ether and active carbon. There is obtained ethyl ^ 10 3,4-dihydroxy-5-nitrophenylglyoxylate of m.p. 77-79° (from ether/n-hexane).
In an analogous manner: e) From methyl 4-hydroxy-3-methoxy-5-nitrophenyl- glyoxylate there is obtained methyl 3.4-dihydroxy-5-nitro-phenylglyoxylate as a yellow distillate at 145-150° and 10.67 Pa. f) from isopropyl 4-hydroxy-3-methoxy-5-nitrophenyl- glyoxylate there is obtained isopropyl 3,4-dihydroxy-5--nitrophenylglyoxylate as a yellow distillate at 155-160° and 12.0 Pa, ^ 25 5) from n-butyl 4-hydroxy-3-methoxy-5-nitrophenyl- glyoxylate there is obtained n-butyl 3.4-dihydroxy-5--nitrophenylglyoxylate as a yellow distillate at 160-165° and 10.67 Pa and h) from n-hexyl 4-hydroxy-3-methoxy-5-nitrophenyl- glyoxylate there is obtained hexyl 3.4-dihydroxy-5-nitro-phenylglyoxylate as a yellow distillate at 165-170° and 12.0 Pa.
Example 13 236.1 mg of n-hexyl 3,4-dihydroxy-nitrophenyl- glyoxylate ace dissolved in 15 ml of ethanol and treated with 7.59 ml of 0.1N sodium hydroxide solution. After one hour the reddish-yellow solution is evaporated. The 5 resulting sodium salt of the n-hexyl 3.4-dihydroxy-5- -nitrophenylglyoxylate is crystallized from water and has a m.p. of - 300°.
Example 14 A solution of 3.18 g of n-hexyl 3,4-dihydroxy-5-nitro-phenylglyoxylate in 47.5 ml of ethanol is treated with l.ll g of O-methylhydroxylamine hydrochloride, 1.95 g of sodium acetate and 2.5 ml of water and heated to boiling 15 under reflux for 5 hours. Thereafter, the reaction mixture is evaporated and the residue is treated with ether and water. The organic phase is washed with saturated sodium chloride solution, dried over sodium sulphate, filtered and evaporated. The residue is chromatographed on silica 20 gel with methylene chloride. There is obtained a 7:3 mixture of n-hexyl E- and Z-3.4-dihydroxy-5-nitrophenyl-glyoxylate O-methyl oxime as a reddish oil; 80 MHz NMR spectrum (CDC13): signal for O-methyl at 3.96 and 4.05 ppm.
Example 15 A solution of 5.1 g of ethyl 3,4-dihydroxy-5-nitro-phenylglyoxylate in dry methylene chloride is treated 30 dropwise at -10° within 15 minutes with 25 g of boron tribromide. The mixture is then stirred at -10° for one hour and subsequently at room temperature for 17 hours. Thereafter, the reaction mixture is evaporated, the residue is treated cautiously with water and stirred at 35 50° for a further 30 minutes. After cooling to room temperature the flocculent precipitate is filtered off under suction. The aqueous phase is acidified with 10 ml of IN hydrochloric acid, extracted four times with ether, the combined organic extracts are washed four times with saturated sodium chloride solution, dried over sodium sulphate, filtered and evaporated. The crude product is filtered three times in succession in ether through a filter aid of diatomaceous earth. There is obtained 3,4-dihydroxy-5-nitrophenylglyoxy1ic acid of m.p. 172-174° (from isopropyl ether).
Example 16 a) A mixture of 3.93 g of n-hexyl 3,4-dihydroxy-5-nitro-phenylglyoxylate and 1.38 g of 2-aminophenol is melted at 120° while stirring. The melt crystallizes after 5 minutes. After 2 hours it is cooled and recrystallized from methanol. There is obtained 3 — (3,4-dihydroxy-5-nitro-phenyl)-2H-l,4-benzoxazin-2-one of m.p. 202-204°.
In an analogous manner: b) From n-hexyl 3,4-dihydroxy-5-nitrophenylglyoxylate and 2-amino-p-cresol there is obtained 3-(3,4-dihydroxy-5--nitrophenyl)-6-methyl-2H-l,4-benzoxazin-2-one of m.p. 233-235° (from methanol/methylene chloride), c) from n-hexyl 3,4-dihydroxy-5-nitrophenylglyoxylate and 2-amino-4-propylphenol there is obtained 3-(3,4-dihydroxy--5-nitrophenyl)-6-propyl-2H-l,4-benzoxazin-2-one of m.p. 200-202° (from methanol), d) from n-hexyl 3,4-dihydroxy-5-nitrophenylglyoxylate and 3-amino-4-hydroxybenzoic acid there is obtained 3-(3,4--dihydroxy-b-nitrophenyl)-2-oxo-2H-l,4-benzoxazine-6--carboxylic acid of m.p. 286-287° (from acetone/petroleum ether), II 2 e) from n-hexyl 3.4-dihydroxy-5-nitrophenylglyoxylate and 2-amino-4-chlorophenol there is obtained 6-chloro-3- -(3.4-dihydroxy-5-nitrophenyl)-2H-1,4-benzoxazin-2-one of 5 m.p. 241-243° (from methanol), f) from n-hexyl 3,4-dihydroxy-5-nitrophenylglyoxylate and 2-amino-4.6-dichlorophenol there is obtained 6.8-dichloro--3 -(3,4-d ihydroxy-5-nitrophenyl)-2H-1,4-benzoxazin-2-one of m.p. 237-239° (from ethanol/ether) and g) from n-hexyl 3,4-dihydroxy-5-nitrophenylglyoxylate and 2-amino-5-nitrophenol there is obtained 3-(3,4-dihydroxy--5-nitrophenyl)-7-nitro-2H-1.4-benzoxazin-2-one of m.p. 253-255° (from acetonitrile/ethanol).
Example 17 a) A mixture of 396.0 mg of n-hexyl 3,4-dihydroxy-5-20 -nitrophenylglyoxylate and 137.6 mg of 1,2-phenylene- diamine is heated to 120° for 60 minutes. Thereafter, the mixture is suspended in methanol, filtered off under suction and recrystallized from N.N-dimethylformamide/ water. There is obtained 3-(3,4-dihydroxy-5-nitrophenyl)~ 25 -2(lH)-quinoxalinone of m.p. >300°.
In an analogous manner: b) From n-hexyl 3.4-dihydroxy-5-nitrophenylglyoxylate and 30 N-methyl-1.2-phenylene diamine there is obtained l-methyl--3-(3,4-dihydroxy-5-nitrophenyl)-2(1H)-quinoxalinone of m.p. 271-273° (from methanol). c) from n-hexyl 3,4-dihydroxy-5-nitrophenylglyoxylate and 35 N-propyl-1,2-phenylene diamine there is obtained l-propyl- -3-(3,4-dihydroxy-5-nitrophenyl)-2(1H)-quinoxalinone of m.p. 183-185° (from methanol). d) from n-hexyl 3.4-dihydroxy-5-nitrophenylglyoxylate and 4.5-dimethy1-1.2-phenylenediamine there is obtained 3-(3,4-dihydroxy-5-nitrophenyl)-6,7-dimethyl-2(1H)--quinoxalinone of m.p. >300° (from N.N-dimethylformamide/ wa t e r). e) from n-hexyl 3.4-dihydroxy-5-nitrophenylglyoxylate and 4.5-dichloro-l.2-phenylenediamine there is obtained 6.7-dichloro-3-(3,4-dihydroxy-5-nitrophenyl)-2(1H)--quinoxalinone of m.p. >300° (from N.N-dimethylformamide/ water), f) from n-hexyl 3,4-dihydroxy-5-nitrophenylglyoxylate and 3-chloro-5-trifluoromethyl-1,2-phenylenediamine there is obtained a 1:1 mixture of 8(and 5)-chloro-3-(3,4--dihydroxy-5-nitrophenyl)-6 (and 7)-trifluoromethyl-2(1H)--quinoxalinone of m.p. >300° (from N,N-dimethylformamide/ water), g) from n-hexyl 3,4-dihydroxy-5-nitrophenylglyoxylate and 4-methoxy-l,2-phenylenediamine there is obtained a 1:1 mixture of 3-(3,4-dihydroxy-5-nitrophenyl)-6(and 7)-methoxy-2(lH)-quinoxalinone of m.p. >300° (from ethanol/ether). h) from n-hexyl 3.4-dihydroxy-5-nitrophenylglyoxylate and 4-nitro-l.2-phenylenediamine there is obtained 1:1 mixture of 3-(3.4-dihydroxy-5-nitrophenyl)-6(and 7)-nitro-2(1H)--quinoxalinone of m.p. >300° (from N.N-dimethylformamide/ water) and i) from n-hexyl 3.4-dihydroxy-5-nitrophenylglyoxylate and N-hexyl-l.2-phenylenediamine there is obtained l-hexyl-3-(3,4-dihydroxy-5-ni trophenyl)-2(1H)-quinoxalinone of m.p. 152-154° (from methanol).
Example 18 A solution of 1.07 g of n-hexyl 3,4-dihydroxy-5-nitro-phenylglyoxylate and 696.3 mg of 2.3-diaminonaphthalene in 3 ml of 1-hexanol is heated to boiling under reflux for 3 hours. The reaction mixture is then cooled and diluted with methanol. The crude product is filtered off under suction and recrystallized from N.N-dimethylformamide/ water. There is obtained 3-(3,4-dihydroxy-5-nitrophenyl)-benzo[g]quinoxalin-2(lH)-one of m.p. >300°.
Example 19 A suspension of 2.0b g of n-hexyl 3,4-dihydroxy-5--nitrophenylglyoxylate and 600.8 mg of thiosemicarbazide is stirred intensively at 90° for 30 minutes. The mixture is then cooled to 40° and treated with a solution of 870.1 mg of sodium hydroxide in 15 ml of water. The solution is subsequently heated to 90° for 30 minutes, cooled to room temperature and treated dropwise with 2 ml of conc. hydrochloric acid. The crystallized-out product is filtered off under suction and then dissolved in ethyl acetate. The solution is washed with saturated sodium chloride solution, dried over sodium sulphate, filtered and evaporated. There is obtained 6-(3,4-dihydroxy-5-nitrophenyl)-3 -mercapto-1,2,4-triazin--5(4H)-one of m.p. 282-284° (from ethanol).
Example 20 aaa) A suspension of 2.9 g of 2-bromo-41-hydroxy-3'--methoxy-5'-nitroacetophenone and 761.2 mg of thiourea in 170 ml of ethanol is treated at 60° with 820.3 mg of sodium acetate and stirred for 6 hours. The reaction mixture is then evaporated, the residue is treated with 170 ml of water and heated to 60° for 30 minutes. After cooling the product is filtered off under suction and washed with water. There is obtained 4-(2-amino-4--thiazolyl)-2-methoxy-6-nitrophenol of m.p. 248-250° (from 5 ethanol). in an analogous manner: aab) From 2-bromo-4'-hydroxy-3'-methoxy-5'-nitroaceto-10 phenone and N-phenylthiourea there is obtained 4-(2-anilino-4-thiazolyl)-2-methoxy-6-nitrophenol of m.p. 185-187° (from ether). aba) 50 ml of 1.2-dichloroethane and 3.56 g of calcium 15 carbonate are added to a solution of 4.88 g of 6-amino-4'- -(trifluoromethyl)-hexanilide hydrochloride in 70 ml of water. The suspension is treated within 60 minutes while stirring at room temperature with a solution of 2.6 g of thiophosgene in 1.7 ml of toluene. After 16 hours the 2o precipitate is filtered off under suction and the organic phase is washed with IN hydrochloric acid and water. After drying over sodium sulphate and filtration the filtrate is evaporated. There is obtained crude 4'-(trifluoromethyl)-hexananilide-6-isothiocyanate. abb) A solution of 5.2 g of crude 4'-(trifluoro-methyl)hexananilide-6-isothiocyanate in 60 ml of ethanol is treated with 100 ml of conc. ammonia solution. After 30 minutes the reaction mixture is evaporated. The residue is dissolved in ethyl acetate, washed with water, dried over sodium sulphate, filtered and evaporated. There is obtained l-[5-[(a,a,a-trifluoro-p-tolyl)carbamoyl]-pentyl]-2-thiourea of m.p. 140-142° (from ethanol). abc) A suspension of 666.7 mg of l-[5-[a,ct,a- -trifluoro-p-tolyl)carbamoyl]pentyl]-2-thiourea and 580.2 mg of 2-bromo-41-hydroxy-3'-methoxy-51-nitroaceto- phenone in 50 ml of ethanol is treated at 60° with 164.2 mg of sodium acetate. A reddish-yellow solution thereby results. After 90 minutes the reaction mixture is evaporated, the residue is treated with water, the precipitate is filtered off under suction and washed four times with 10 ml of water each time. There is obtained 6-[[4-(4-hydroxy-3-methoxy-5-nitrophenyl)-2 -thiazolyl]-amino]-4'-(trifluoromethyl)hexananilide of m.p. 160-162° (from ethanol). ac) A solution of 29.0 g of 2-bromo-4'-hydroxy-3'-methoxy--5'-nitroacetophenone and 13.5 g of 2-aminoacetophenone in 250 ml of dry N.N-dimethylformamide is stirred at 90° for 16 hours. The reaction mixture is then evaporated to about two thirds and poured on to ice. The separated crystals are filtered off under suction and dissolved in methylene chloride. The solution is washed with saturated sodium chloride solution, dried over sodium sulphate, filtered and evaporated. There is obtained 2-(4-hydroxy-3-methoxy--5-nitrobenzoyl)-3-methylindole of m.p. 195-197° (from methylene chloride/methanol). ada) A suspension of 14.5 g of 2-bromo-4'-hydroxy-31 --methoxy-5•-nitroacetophenone and 5.41 g of 1,2-phenylenediamine in 350 ml of methanol is treated with 4.92 g of sodium acetate and the mixture is heated to boiling under reflux for 22 hours. The reaction mixture is then evaporated, the residue is dissolved in methylene chloride, this solution is washed four times with water, dried over sodium sulphate, filtered and evaporated. There is obtained 2-(4-hydroxy-3-methoxy-5-nitrophenyl)quinoxaline of m.p. 195-197° (from methylene chloride/methanol).
In an analogous manner: adb) From 2-bromo-4'-hydroxy-31-methoxy-5'-nitroaceto- .? 1Q4 phenone and 4,5-dimethyl-l,2-phenylenediamine there is obtained 6,7-dimethyl-2-(4-hydroxy-3-methoxy-5 -nitro-phenyl)quinoxaline of m.p. 207-209° (from methylene 5 chloride/methanol). b) A suspension of 267.3 mg of 4-(2-amino-4-thiazolyl)-2--methoxy-6-nitrophenol in 10 ml of dry methylene chloride is treated at -20° with 1.25 g of boron tribromide. After the addition the mixture is stirred at -20° for a further one hour and at room temperature without cooling for 18 hours. The reaction mixture is then evaporated, the residue is treated cautiously with water and stirred at 50° for 30 minutes. After cooling to room temperature the 15 mixture is suction filtered and the crude product is filtered off and washed with a small amount of water.
There is obtained 5-(2-amino-4-thiazolyl)-3-nitropyro-catechol hydrobromide of m.p. 244-246° (from methanol). 2o in an analogous manner: c) From 4-(2-anilino-4-thiazolyl)-2-methoxy-6-nitrophenol there is obtained 5-(2-anilino-4-thiazolyl)-3-nitropyro-catechol of m.p. 202-204° (from methanol), C ^ 25 d) from 6-[4-(4-hydroxy-3-methoxy-5-nitrophenyl)-2- -thiazolyl)amino]-4'-(trifluoromethyl)hexananilide there is obtained 6-[[4-(3,4-dihydroxy-5-nitrophenyl)-2--thiazolyl]amino]-41-(trifluoromethyl)hexananilide of m.p. ^ 30 214-216° (from methanol), e) from 2-(4-hydroxy-3-methoxy-5-nitrobenzoyl)-3--methylindole there is obtained 5-bromo-2-(3,4-dihydroxy--5-nitrobenzoyl)-3-methylindole of m.p. 265-267° (from methanol). f) from 2-(4-hydroxy-3-methoxy-5-nitrophenyl)quinoxaline there is obtained 2-(3.4-dihydroxy-5-nitrophenyl)-quinoxaline of m.p. 241-243° (from methanol) and g) from 6.7-dimethyl-2-(4-hydroxy-3-methoxy-5-nitro-phenyl)quinoxaline there is obtained 6,7-dimethyl-2-(3,4--dihydroxy-5-nitrophenyl)quinoxaline of m.p. 274-276° (from methanol).
Example 21 A mixture of 3.12 g of 2-bromo-34•-dihydroxy-5'--nitroacetophenone and 849.3 mg of thioacetamide are heated to boiling under reflux for 18 hours in 40 ml of ethanol. After cooling the crystals are filtered off under suction and recrystallized from methanol/isopropanol.
There is obtained 5-(2-methyl-4-thiazolyl)-3-nitropyro-catechol hydrobromide of m.p. 280-282°.
Example 22 A solution of 1.38 g of 2-bromo-3'.4'-dihydroxy-5'--nitroacetophenone and 461 rag of thiosemicarbazide in 20 ml of n-butanol is heated to boiling under reflux for 60 minutes. After cooling to room temperature the crystals are filtered off under suction and recrystallized from n-butanol. There is obtained 5-(2-amino-6H-l.3.4--thiadiazin-5-yl)-3-nitropyrocatechol hydrobromide of m.p. 265-267°.
Example 23 A solution of 1.38 g of 2-bromo-341-dihydroxy-5»--nitroacetophenone and 505.7 mg of 2-amino-l,3,4--thiadiazole in 25 ml of n-butanol is heated to boiling under reflux for 7 hours. The reaction mixture is then cooled to room temperature and the separated crystals are 2 194 9 — /»s filtered off under suction. There is obtained 5-(imidazo-[2,1-b]-1,3.4-thiadiazol-6-yl)-3-nitropyrocatechol of m.p. 278-280° (from methanol).
Example 24 A mixture of 2.78 g of 2-brorao-34'-dihydroxy-5•--nitroacetophenone. 1.01 g of 2-aminothiazole and 40 ml of ethanol is heated to boiling under reflux for 23 hours. The reaction mixture is then cooled to room temperature and the crystals are filtered off under suction. There is obtained 5-(imidazo[2.1-b]thiazol-6-yl)-3-nitropyrocatechol hydrobromide of m.p. 286-288° (from methanol).
Example 25 A mixture of 1.95 g of 2-bromo-34'-dihydroxy-5 ' --nitroacetophenone. 1.06 g of 2-aminobenzothiazole and 50 ml of ethanol is heated to boiling under reflux for 17 hours. The reaction mixture is then cooled to room temperature, whereupon the crystals are filtered off under suction. There is obtained 5-(imidazo[2.l-b]benzothiazol--2-yl)-3-nitropyrocatechol of m.p. 303-305° (from N.N-dimethylformamide/methano 1).
Example 26 A mixture of 2.78 g of 2-bromo-341-dihydroxy-5•--nitroacetophenone, 1.51 g of 2-aminothiophenol and 50 ml of ethanol is heated to boiling under reflux for one hour. The reaction mixture is then cooled to room temperature, whereupon the crystals are filtered off under suction. There is obtained 5-(2H-l,4-benzothiazin-3-yl)-3-nitro-pyrocatechol of m.p. 302-304° (from N.N-dimethyl-formamide/methanol). vi18^ * Example 27 2 /1 r> » A mixture of 987 .5 mg of 2-bromo-3' .4•-dihydroxy-51 --nitroacetophenone and 1.01 g of 2-aminopyridine is melted at 110°. After 30 minutes the melt is treated with 15 ml of ethanol and heated to boiling under reflux for 3 hours. The reaction mixture is then cooled to room temperature and the crystals are filtered off under suction. There is obtained 5-(imidazo[l,2-a]pyridin-3-yl)-3-nitropyro-catechol of m.p. 250-252° (from N.N-dimethylformamide/-methanol).
Example 28 a) 8.9 g of 1.1•-carbonyldiimidazole are added to a solution of 10.7 g of 4-hydroxy-3-methoxy-5-nitrobenzoic acid in 500 ml of dry tetrahydrofuran and the reaction mixture is subsequently heated to 65-70° for 5 hours. It is then cooled to room temperature and a solution of 21.6 g of 6-aminohexyl-t-butylcarbamate in 50 ml of dry tetrahydrofuran is added dropwise thereto within 15 minutes. The reaction mixture is then heated to 65-70° for 18 hours, evaporated, the residue is suspended in ethyl acetate, suction filtered and the suction filtered material is chromatographed on silica gel with acetone/methylene chloride (3:1). There is obtained [6-(4-hydroxy-5-ni tro-m-anisamido)hexyl]-t-butylcarbamate of m.p. 145-147° (from isopropanol). b) 5.8 ml of hydrobromic acid in glacial acetic acid (~33 percent) are added at room temperature to a solution of 4.1 g of [6-(4-hydroxy-5-nitro-m-anisamido)-hexyl]-t-butylcarbamate in 80 ml of glacial acetic acid. The mixture is stirred for 2 hours, the separated crystals are filtered off under suction and washed with ether.
There is obtained N-(6-aminohexyl)-4-hydroxy-5-nitro-m- W&g-K*i'?Lti{--'C'A'-1'-:>\c^^£^£0 ' -anisamide hydrobromide of m.p. 207-209° (from isopropanol). c) 1.25 g of boron tribroraide are added -20° to a suspension of 392.3 mg of N-(6-aminohexyl)-4-hydroxy-5--nitro-m-anisamide hydrobromide in 25 ml of dry methylene chloride. After the addition the mixture is stirred at -20° for one hour and subsequently at room temperature for 17 hours. The reaction mixture is then evaporated, the residue is treated with 10 ml of water and stirred at room temperature for one hour. After evaporating the water the residue is chromatographed on Sephadex LH 20 with toluene/ethanol (1:1). There is obtained N-6-aminohexyl--3,4-dihydroxy-5-nitrobenzamide hydrobromide of m.p. 205-207° (from ethanol).
Example 29 aa) A solution of 4.93 g of 5-nitrovanillin and 2.7 g of 1.2-phenylenediamine in 45 ml of methanol and 15 ml of nitrobenzene is heated to boiling under reflux. After 15 minutes crystals begin to separate from the red solution. After 18 hours the reaction mixture is cooled to room temperature and diluted with 60 ml of methanol. The crystals are filtered off under suction and washed with methanol. There is obtained 4-(2-benzimidazolyl)-2--methoxy-6-nitrophenol of m.p. 198-200° (from N,N-dimethyIformamide/methanol). in an analogous manner: ab) From 5-nitrovanillin and 4,5-dichloro-l.2-phenylene-diamine there is obtained 4-(5,6-dichloro-2-benz-imidazolyl)-2-methoxy-6-nitrophenol of m.p. 258-260° (from N.N-dimethylformamide/ether). 2 b) A suspension of 860.1 rag of 4-(2-benzimidazolyl)-2--methoxy-6-nitrophenol in 10 ml of glacial acetic acid and 10 ml of 48 percent hydrobromic acid is heated to boiling 5 under reflux for 72 hours. The mixture is then evaporated and the residue is treated four times with 50 ml of toluene each time, which is again distilled off each time. There is obtained 5-(2-benzimidazolyl)-3-nitropyrocatechol of m.p. >300° (from acetone/water).
In an analogous manner: c) From 4-(5.6-dichloro-2-benzimidazolyl)-2-methoxy-6--nitrophenol there is obtained 5-(5,6-dichloro-2-15 -benzimidazolyl)-3-nitropyrocatechol of m.p. 282-284° (from acetone/water).
Example 30 A suspension of 29.0 g of 2-bromo-4•-hydroxy-3•- -methoxy-5'-nitroacetophenone in 700 ml of dry methylene chloride is treated at -20° within 30 minutes with a solution of 125.3 g of boron tribromide in 300 ml of dry methylene chloride. After the addition the mixture is 25 stirred at -20° for a further one hour and at room temperature for 16 hours, then evaporated, the residue is treated cautiously with water while cooling with ice and stirred at 50° for 30 minutes. After cooling the mixture is extracted with ether, the ethereal phase is washed with 30 water, dried over sodium sulphate, filtered and evaporated. There is obtained 2-bromo-3',41-dihydroxy-5'-nitroacetophenone of m.p. 138-140° (from methylene chloride).
Example 31 a) A solution of 3.6 g of ethyl 3,4-dihydroxy-5-nitro-phenylglyoxylate in 30 ml of acetic anhydride is heated at G r^ 4 <r% A £ 1 / ^ 110° for 30 minutes in the presence of a catalytic amount of conc. sulphuric acid, cooled to room temperature, the reaction mixture is poured into 150 ml of water and 5 stirred for 60 minutes. The mixture is extracted with ether, washed with saturated sodium chloride solution, the combined organic extracts are dried sodium sulphate, filtered and evaporated. There is obtained ethyl 3,4-diacetoxy-5-nitrophenylglyoxylate of m.p. 87-89° (from 10 ether/petroleum ether).
In an analogous manner: b) From 3-(3,4-dihydroxy-5-nitrophenyl)-2H-1.4- -benzoxazin-2-one there is obtained 3-(3.4-diacetoxy-5--nitrophenyl)-2H-l.4-benzoxazin-2-one of m.p. 186-188° (from methylene chloride/methanol), c) from 3-(3,4-dihydroxy-5-nitrophenyl)-2(lH)- -quinoxalinone there is obtained 3-(3,4-diacetoxy-5-nitro-phenyl)-2(1H)-quinoxalinone of m.p. 241-243° (from methylene chloride/methanol), d) from 3.4-dihydroxy-5-nitrobenzophenone there is 25 obtained 3.4-diacetoxy-5-nitrobenzophenone of m.p. 141-143° (from methylene chloride/ether). e) from 2'-fluoro-3,4-dihydroxy-5-nitrobenzophenone there is obtained 3,4-diacetoxy-21-fluoro-5-nitrobenzophenone of m.p. 122-124° (from ether) and f) from 3.4-dihydroxy-5-nitrophenyl 4-pyridyl ketone there is obtained 3.4-diacetoxy-5-nitrophenyl 4-pyridyl ketone of m.p. 148-150° (from methylene chloride/ether).
Example 32 a) A solution of 2.0 g of 3,5-dinitropyrocatechol in 25 ml of propionic anhydride is heated at 110° for 18 hours in the presence of a catalytic amount of conc. sulphuric acid, the excess anhydride is distilled off at 70° in a high vacuum (1.33 Pa), the residue is dissolved in methylene chloride, washed with water, dried over sodium sulphate, filtered and evaporated. There is obtained 1.2-dipropionyloxy-3,5-dinitrobenzene of m.p. 74-76° (from methylene chloride/petroleum ether).
In an analogous manner: b) From 3-(3,4-dihydroxy-5-nitrophenyl)-6-methyl-2H-1.4--benzoxazin-2-one there is obtained 3-(3,4-dipropionyloxy--5-nitrophenyl)-6-methyl-2H-l,4-benzoxazin-2-one of m.p. 158-160° (from methylene chloride), c) from 6-chloro-3-(3.4-dihydroxy-5-nitrophenyl)-2H-1,4--benzoxazin-2-one there is obtained 5-(6-chloro-2-oxo-2H--1.4-benzoxazin-3-yl)-3-nitro-o-phenylene-dipropionate of m.p. 148-150° (from methylene chloride/ether), d) from 3-(3,4-dihydroxy-5-nitrophenyl)-7-nitro-2H-l,4--benzoxazin-2-one there is obtained 3-nitro-5-(7-nitro-2--oxo-2H-l.4-benzoxazin-3-yl)-o-phenylene-dipropionate of m.p. 177-179° (from methanol). e) from 3-(3,4-dihydroxy-5-nitrophenyl)-2-oxo-2H-l,4--benzoxazine-6-carboxylic acid there is obtained 3-[3,4--bis(propionyloxy)-5-nitrophenyl]-2-oxo-2H-1,4-benzoxazine -6-carboxylic acid of m.p. 192-194° (from methylene chloride), f) from 3-nitro-5-(2-quinoxaiinyl)pyrocatechol there is obtained 3-nitro-5-(2-quinoxalinyl)-o-phenylene--dipcopionate of m.p. 152-154° (from methylene chloride/-ether, g) from 5-(2-benzimidazolyl)-3-nitropyrocatechol there is obtained 5-(2-benzimidazolyl)-3-nitro-o-phenylene--dipropionate of m.p. 179-181° (from ether). h) from 6,7-dichloro-3-(3, 4-d ihydroxy-5-ni trophenyl)--2(1H)-quinoxalinone there is obtained 5-(6.7-dichloro--3.4-dihydro-3-oxo-2-quinoxalinyl)-3-nitro-o-phenylene--dipropionate of m.p. 260-262° (from methylene chloride). i) from 5-bromo-2-(3,4-dihydroxy-5-nitrobenzoyl)-3--methylindole there is obtained 5-bromo-2-(3,4--dipropionyloxy-5-nitrobenzoyl)-3-raethylindole of m.p. 196-198° (from ether) and j) from 6-(3,4-dihydroxy-5-nitrophenyl)-3-mercapto-l,2,4--triazin-5(4H)-one there is obtained 3-nitro-5-(2,3,4,5--tetrahydro-5-oxo-3-thioxo-as-triazin-6-yl)-o-phenylene--dipropionate of m.p. 237-239° (from ether). { . 25 Example 33 a) A solution of 1.09 g of ethyl 3,4-dihydroxy-5-nitro-phenylglyoxylate in 6 ml of isobutyric anhydride is heated at 110° for 17 hours in the presence of a catalytic amount 30 of conc. sulphuric acid. The reaction mixture is then treated ten times with 10 ml of toluene each time, whereby it is evaporated each time at 80° and 18.7 mbar. The oily residue is distilled in a bulb-tube at 175-180° and 8.0 Pa. There is obtained ethyl 3.4-diisobutyryloxy-5-35 -nitrophenylglyoxylate.
In an analogous manner: * o ^ ./I,' 2 b) From 3,5-dinitropyrocatechol there is obtained 1.2--diisobutyryloxy-3,5-dinitrobenzene of m.p. 78-80° (from ether). c) from 3-(3.4-dihydroxy-5-nitrophenyl)-6-methyl-2H-1.4--benzoxazin-2-one there is obtained 3-(3.4-diisobutyryl-oxy-5-nitrophenyl)-6-methyl-2H-l.4-benzoxazin-2-one of m.p. 142-144° (from ether). d) from 2-(3,4-dihydroxy-5-nitrophenyl)quinoxaline there is obtained 2-(3,4-diisobutyryloxy-5-nitrophenyl)-quinoxaline of m.p. 155-157° (from ether), e) from l-methyl-3-(3.4-dihydroxy-5-nitrophenyl)-2(lH)--quinoxalinone there is obtained l-methyl-3-(3,4--diisobutyryloxy-5-nitrophenyl)-2(1H)-quinoxalinone of m.p. 138-140° (from ether), f) from 5-(imidazo[2.1-b]-1,3.4-thiadiazol-6-yl)-3-nitro pyrocatechol there is obtained 5-(imidazo[2.1-b]-1.3.4--thiadiazol-6-yl)-3-nitro-o-phenylene diisobutyrate of m.p. 169-171° (from methylene chloride), U 25 9) from 2-bromo-31.4•-dihydroxy-51-nitroacetophenone there is obtained 5-(bromoacetyl)-3-nitro-o-phenylene diisobutyrate of m.p. 56-58° (from methylene chloride/ hexane), h) from 5-(2-amino-4-thiazolyl)-3-nitropyrocatechol hydrobromide there is obtained 3-nitro-5-(2-isobutyramido -4-thiazolyl)-o-phenylene diisobutyrate of m.p. 157-159° (from methylene chloride/ether), i) from 5-(2-amino-6H-l,3.4-thiadiazin-5-yl)-3-nitro- pyrocatechol hydrobromide there is obtained 3-nitro-5-(2--isobutyramido-4-isobutyryl-l.3,4-thiadiazin-5-yl)-o- - 59 2 4/ ^ -phenylene diisobutyrate of m.p. 177-179° (from ether). j) from 3-(3,4-dihydroxy-5-nitrophenyl)-6-propyl-2H-1,4-5 -beri2oxazin-2-one there is obtained 3-nitro-5-(2-oxo-6- -propyl-2H-l,4-benzoxazin-3-yl)-o-phenylene diisobutyrate of m.p. 131-133° (from methanol), k) from 5-(imidazo[l,2-a]pyridin-3-yl)-3-nitropyro-10 catechol there is obtained 5-(imidazo[1.2-a]pyridin-3-yl)--3-nitro-o-phenylene diisobutyrate of m.p. 137-139° (from ether), 1) from 5-(imidazo[2,l-b]benzothiazol-2-yl)-3-nitropyro-15 catechol there is obtained 5-(imidazo[2.1-b]benzothiazol--2-yl)-3-nitro-o-phenylene diisobutyrate of m.p. 197-199° (from methylene chloride/ether and m) from 3.4-dihydroxy-5-nitrophenyl 2-pyridyl ketone 20 hydrobromide there is obtained 3-nitro-5-(2-pyridyl- carbonyl)-o-phenylene diisobutyrate of m.p. 83-85° (from ether/hexane).
Example 34 a) A solution of 613.0 mg of ethyl 3,4-dihydroxy-5-nitro-phenylglyoxylate in 4 ml of pivaloyl anhydride is heated to 100° for 17 hours in the presence of a catalytic amount of conc. sulphuric acid, the cooled solution is diluted 30 with ether, washed with saturated sodium solution, dried over sodium sulphate, filtered and evaporated. The residue is treated ten times with 10 ml of toluene, whereby it is evaporated again each time. After bulb-tube distillation (air-bath) at 175-180° and 4.0 Pa there is obtained ethyl 35 3,4-dipivaloxyloxy-5-nitrophenylglyoxylate.
In an analogous manner: b) From 3-(3.4-dihydroxy-5-nitrophenyl)-6-methy1-2H-1.4--ben2oxazin-2-one there is obtained 3-(3,4-dipivaloyloxy--5-nitrophenyl) -6-methyl-2H-l,4-benzoxazin-2-one of m.p. 180-192° (from ether), c) from 3.4-dihydroxy-5-nitrobenzophenone there is obtained 3,4-dipivaloyloxy-5-nitrobenzophenone of m.p. 101-103° (from t-butyl methyl ether) and d) from 2'-fluoro-3,4-dihydroxy-5-nitrobenzophenone there is obtained 3,4-dipivaloyloxy-2'-fluorobenzophenone of m.p. 74-76° (from low-boiling petroleum ether).
Example 35 A solution of 1.7 g of 3-(3.4-dihydroxy-5-nitrophenyl )-2(1H)-quinoxalinone in 17 ml of oenanthic anhydride is heated to 110° for 17 hours in the presence of a catalytic amount of conc. sulphuric acid, the excess anhydride is then distilled off in a high vacuum, the residue is dissolved in methylene chloride, the organic solution is washed with water, dried over sodium sulphate, filtered and evaporated. There is obtained 5-(1,2-dihydro--2-oxo-3-quinoxalinyl)-3-nitro-o-phenylene diheptanoate of m.p. 186-188° (from methylene chloride/ether).
Example 36 a) 1.26 g of sodium acetate are added to a solution of 1.3b g of 2-bromo-3',41-dihydroxy-5'-nitroacetophenone in 2b ml of alcohol and heated to boiling under reflux. After 6 hours the reaction mixture is filtered off from separated sodium bromide and evaporated. The residue is dissolved in ethyl acetate. The solution is washed with saturated sodium chloride solution, dried over sodium sulphate, filtered and evaporated at 50°. There is #1 A g obtained (3.4-dihydroxy-5-nitrobenzoyl)methyl acetate of % m.p. 166-168° (from ethyl acetate/ether). 1 si r _ In an analogous manner: b) from 2-bromo-3' .41-dihydroxy-51-nitroacetophenone and sodium isobutyrate there is obtained (-3.4-dihydroxy-5--nitrobenzoyl)methyl isobutyrate of m.p. 120-122° (from ethyl acetate/ether) and c) from 2-brorao-341-dihydroxy-5'-nitroacetophenone and sodium 3,4-dihydroxy-5-nitrophenylglyoxylate there is obtained 3,4-dihydroxy-5-nitrophenacyl (3.4-dihydroxy-5--nitrobenzoyl)formate of m.p. 224-226° (from methanol/ ethyl acetate).
Example 37 A suspension of 2.91 g of 2-bromo-3'.4'-dihydroxy-5'--nitroacetophenone is treated with 1.31 g of thionicotin-amide in 50 ml of alcohol and heated to boiling under reflux for 2 hours. After cooling to room temperature the crystals are filtered off under suction and recrystallized from N.N-dimethylformamide/alcohol. There is obtained 3-nitro-5-[2-(3-pyridyl)-4-thiazolyl]pyrocatechol of m.p. 279-281°.
Example 38 A solution of 5.52 g of 2-bromo-34•-dihydroxy-5'--nitroacetophenone and 2.7 g of 2-aminoacetophenone in 100 ml of dry N.N-dimethylformamide is stirred at 90° for 24 hours. The reaction mixture is evaporated, the residue is dissolved in ethyl acetate, washed with water, dried over sodium sulphate, filtered and evaporated. There is obtained 2-(3,4-dihydroxy-5-nitrobenzoyl)-3-methy1 indole -" - 2 19496 of m.p. 212-214° (from n-butanol).
Example 39 A suspension of 7.32 g of 2-bromo-31,4'-dihydroxy-5'--nitroacetophenone is treated with 4.06 g of l-(3--pyridinyl)-2-thiourea in 100 ml of n-butanol and heated to boiling under reflux for 3 hours. After cooling to room temperature the crystals are filtered off under suction and recrystallized from n-butanol. There is obtained 3-nitro-5-[2-(3-pyridylamino)-4-thiazolyljpyrocatechol hydrobromide of m.p. >300°.
Example 40 A suspension of 6.35 g of 2-bromo-34'-dihydroxy-5'--nitroacetophenone is treated with 4.68 g of l-(3--quinolinyl)-2-thiourea in 150 ml of n-butanol and heated to boiling under reflux for 3 hours. After cooling to room temperature the crystals are filtered off under suction and recrystallized from n-butanol. There is obtained 3-nitro-5-[2-(3-quinolinylamino)-4-thiazolyl]pyrocatecho1 hydrobromide of m.p. >300°.
Example 41 A suspension of 8.28 g of 2-bromo-34■-dihydroxy-5'--nitroacetophenone is treated with 6.37 g of rac-l-(2-exo--bornyl)-2-thiourea in 100 ml of n-butanol and heated to boiling under reflux for 3 hours. After cooling to room temperature the crystals are filtered of £ under suction and recrystallized from n-butanol. There is obtained rac-3-nitro-5-[2-(2-exo-bornylamino)-4-thiazolyl]-pyro-catechol hydrobromide of m.p. 262-264°.
Example 42 0.875 ml of pyrrolidine in 35 ml of tetrahydrofuran is treated at 5° with 0.605 ml of acetic acid and subsequently with 1.73 g of 3.4-dihydroxy-5-nitrobenz-aldehyde and 2.87 g of 6-oxo-4'-(trifluoromethyl)-heptananilide and stirred at 23° under argon for 56 hours. The residue obtained after evaporating the reaction mixture is partitioned between ethyl acetate and IN sodium hydroxide solution. The combined sodium hydroxide extracts are made acid with conc. hydrochloric acid, extracted with ethyl acetate, the combined ethyl acetate extracts are washed with saturated sodium chloride solution, dried over sodium sulphate, evaporated and the residue is chromato-graphed on 120 g of silica gel with methylene chloride/ methanol (91:9). After recrystallization from ethyl acetate/petroleum ether there is obtained (E)-8-(3.4--dihydroxy-5-nitrophenyl)-6-0x0-4'-(trifluoromethyl)-7--octenanilide of m.p. 194-197°.
Example 43 a) 26.0 g of 2-chloro-3-hydroxy-p-anisaldehyde are dissolved in 400 ml of acetic anhydride and 5 ml of pyridine. The solution is stirred at 80° for 8 hours, subsequently evaporated, the residue is partitioned between ice-water and methylene chloride, the organic phase is dried over sodium sulphate, evapor- ated and the residue is recrystallized from methylene chloride/petroleum ether. There is obtained 2-chloro-3--formyl-6-methoxyphenyl acetate of m.p. 48-50°. b) 38 g of 2-chloro-3-formyl-6-methoxyphenyl acetate are introduced portionwise at -5° to -10° within 15 minutes into 150 ml of 98 percent nitric acid. After stirring at -5° for 30 minutes the reaction mixture is poured into 1.5 1 of ice-water and extracted three times with 500 ml of methylene chloride. The combined organic phases are washed with ice-water, dried over sodium sulphate and evaporated. The residue is crystallized from ether. There is obtained 2-chloro-3-formyl-6-methoxy-5-nitrophenyl acetate of m.p. 84-85°. c) 35.8 g of 2-chloro-3-formyl-6-methoxy-5-nitrophenyl acetate are dissolved in 300 ml of methanol. After adding 145 ml of IN sodium hydroxide solution the mixture is stirred at 23° for 1 hour. After evaporation of the methanol the residue is diluted with ice-water, made acid with 2N hydrochloric acid and extracted twice with 400 ml of ethyl acetate each time. The organic phases are washed with saturated sodium chloride solution, dried over sodium sulphate and evaporated. The residue is recrystallized from methylene chloride/petroleum ether. There is obtained 2-chloro-3-hydroxy-5-nitro-p-anisaldehyde of m.p. 130°. d) 1.3 g of 2-chloro-3-hydroxy-5-nitro-p-anisaldehyde are dissolved in 80 ml of methylene chloride, treated with 0.82 ml of boron tribromide, stirred at 23° for 18 hours, the reaction mixture is treated with 5 ml of methanol while cooling with ice. evaporated, the residue is dried in a high vacuum, digested in water, filtered and recrystallized from acetonitrile. There is obtained 2-chloro-3.4-dihydroxy-5-nitrobenzaldehyde of m.p. 193-195° .
Example 44 a) A mixture of 30 g of 2-chloro-3-hydroxy-p-anisaldehyde and 230 ml of ethanol is stirred at 70° for 4 hours in the presence of 12.3 g of hydroxylamine hydrochloride, the reaction mixture is subsequently evaporated, the residue is dried in a high vacuum and recrystallized from 2 i £ - 65 - * methanol/water. There is obtained 2-chloro-3-hydroxy-p--anisaldehyde oxime of m.p. 174-176°. b) 21 g of 2-chloro-3-hydroxy-p-anisaldehyde oxime are heated under reflux for 20 hours together with 400 ml of acetic anhydride. Thereupon, the reaction mixture is evaporated, the residue is treated with 300 ml of ice-water, stirred for 1 hour, decanted, the thus-cooled 7 10 residue is partitioned between methylene chloride and water, the organic phase is dried over sodium sulphate, evaporated, the residue is dried in a high vacuum, chromatographed on 200 g of silica gel with methylene chloride and recrystallized from methylene chloride/ 15 petroleum ether. There is obtained 2-chloro-3-cyano-6--methoxyphenyl acetate of m.p. 97-99°. c) In analogy to Examples 43b and 43c, from 2-chloro-3--.cyano-6-methoxyphenyl acetate there is obtained 2-chloro-20 -3-hydroxy-5-nitro-p-anisonitrile of m.p. 157-159° (methylene chloride/hexane). d) In analogy to Example 43d, from 2-chloro-3-hydroxy-5--nitro-p-anisonitrile there is obtained 2-chloro-3,4- f -dihydroxy-5-nitro-benzonitrile of m.p. 180° (acetonitrile).
Example 45 a) 5.5 g of a-chloro-2-fluoro-3,4-dimethoxytoluene and 4.05 g of potassium acetate are stirred at 80° for 25 hours in 50 ml of dimethylformamide. The reaction mixture is subsequently poured into 150 ml of ice-water and extracted with ether. The ether phases are washed with 35 sodium chloride solution, dried over sodium sulphate and evaporated. 2-Fluoro-3.4-dimethoxybenzyl acetate is obtained as an oil.
(J8B5BSS§?!sS®}' c 2 A O f 66 - b) 5.4 g of 2-fluoro-3,4-dimethoxybenzyl acetate ace heated to 80° foe 1.5 houcs together with 50 ml of methanol and 50 ml of IN sodium hydcoxide solution. Aftec evaporation of the methanol the residue is extracted with methylene chloride. The combined extracts are washed with water, dried over sodium sulphate and evaporated. The residue is chromatographed on 80 g of silica gel with methylene chloride/methanol (95:5). 2-Fluoro-3.4--dimethoxybenzyl alcohol is obtained as an oil. c) 3.0 g of 2-fluoro-3.4-dimethoxybenzyl alcohol and 5.0 g of manganese dioxide are heated under reflux for l hour together with 50 ml of benzene. The insoluble constituents are subsequently filtered off while washing with methylene chloride. The filtrate is evaporated and the residue is recrystallized from methylene chloride/ hexane. There is obtained 2-fluoro-3.4-dimethoxybenz-aldehyde of m.p. 52-54°. d) 4.4 g of 2-fluoro-3.4-dimethoxybenzaldehyde and 1.83 g of hydroxylamine hydrochloride are heated under reflux for 5 hours together with 30 ml of ethanol. The reaction mixture is subsequently evaporated and the residue, dried in a high vacuum at 23°, is introduced into 40 ml of phosphorus oxychloride. After stirring at 23° for 2.5 hours the reaction mixture is evaporated, the residue is treated with ice-water, the precipitate which thereby forms is filtered off. washed with watec. taken up in methylene chloride, the methylene chloride solution is dried over sodium sulphate and evaporated. By recrystallization of the residue from methylene chloride/hexane there is obtained 2-fluoro-3,4-dimethoxybenzonitrile of m.p. 64-65° . e) 2.0 g of 2-fluoro-3.4-dimethoxybenzonitrile are dissolved in 60 ml of methylene chloride, treated with 1.1 ml of boron tribromide. stirred at 23° for 1 hour, subsequently treated with a further 1.0 ml of boron tribromide and stirred at 23° for a further 80 minutes. Thereupon, the reaction mixture is poured into 100 ml of ice-cold saturated sodium hydrogen carbonate solution, whereupon the mixture is extracted twice with 300 ml of ether, the combined ether phases are washed twice with sodium chloride solution, dried over sodium sulphate, evaporated and the residue is chromatographed on 50 g of silica gel with methylene chloride and methylene chloride/methanol (97:3). There is obtained 2-fluoro-3~ -hydroxy-p-anisonitrile of m.p. 198-200°. f) 0.9 g of 2-fluoro-3-hydroxy-p-anisonitrile are dissolved in 10 ml of acetic anhydride and 0.5 ml of pyridine, whereupon the mixture is stirred at 120° for 2 hours. The reaction mixture is subsequently evaporated and the residue is partitioned between ice-water and methylene chloride. The organic phase is dried over sodium sulphate and evaporated, and the residue is recrystallized from methylene chloride/hexane. There is obtained 3-cyano-2- -fluoro-6-methoxyphenyl acetate of m.p. 90-91°. g) 0.8 g of 3-cyano-2-fluoro-6-methoxyphenyl acetate are introduced in three portions at -15° into 5 ml of 96 percent nitric acid, whereupon the mixture is stirred at -10° for 1 hour. Thereupon, the reaction mixture is poured on to 50 g of ice. The mixture is extracted twice with 70 ml of ethyl acetate each time. The combined organic phases are washed with saturated sodium chloride solution, dried over sodium sulphate and evaporated. The thus--obtained residue is dissolved in 50 ml of IN sodium carbonate solution and 50 ml of methanol, whereupon the solution is stirred at 23° for 1 hour and the methanol is distilled off. The residal aqueous phase is adjusted to pH 2 at 0° with conc. hydrochloric acid and extracted , : .
X • m 2 1949s twice with 100 ml o£ ethyl acetate. The combined organic i phases are washed with saturated sodium chloride solution. dried over sodium sulphate and evaporated, and the residue I 5 is chromatographed on 45 g of silica gel with methylene ;> chloride/methanol (97:3). After recrystallization from \ ether/hexane there is obtained 2-fluoro-3-hydroxy-5-nitro- f -p-anisonitrile of m.p. 112-113°. | i h) 550 mg of 2-fluoro-3-hydroxy-5-nitro-p-anisonitrile are dissolved in 30 ml of methylene chloride, whereupon the solution is treated with 0.44 ml of boron tribromide.
After stirring at 23° for 6 hours a further 0.1 ml of boron tribromide are added thereto, whereupon the mixture 15 is stirred at 23° for a further 1.5 hours. Thereupon, 3 ml of ethanol are added thereto at -15°. The reaction mixture is evaporated and the residue is dried in a high vacuum at 23°. By recrystallization from ether/hexane there is obtained 2-fluoro-3,4-dihydroxy-5-nitrobenzonitrile of 20 m.p. 154°.
Example 46 a) 9.5 g of 3,4-dimethoxy-5-nitrobenzoic acid are 25 suspended in 95 ml of thionyl chloride. The suspension is stirred at 80° for 1 hour. By two-fold evaporation with the addition of absolute toluene there are obtained 10 g of 3»4-dimethoxy-5-nitrobenzoyl chloride which is dissolved in 100 ml of tetrahydrofuran. This solution is 30 added dropwise to 300 ml of 28 percent aqueous ammonia.
The mixture is subsequently stirred at 40° for 2 hours and cooled to 5°. The crystalline precipitate is filtered off. There is obtained 3,4-dimethoxy-5-nitrobenzamide of m.p. 182-185°. b) 2.46 g of chlorine are introduced while cooling with ice into a mixture of 8.0 g of sodium hydroxide, 50 ml of 4 m K * 2 water and 30 g of ice. Thereupon. 6.5 g of 3,4-dimethoxy--5-nitrobenzamide suspended in 5 ml of tetrahydrofuran are slowly added thereto. The reaction mixture is heated to 5 7o° within 30 minutes, stirred at 70° for 1 hour, cooled to 5° and the separated crystals are filtered off. There is obtained 3,4-dimethoxy-5-nitroaniline of m.p. 129-131°. By extraction of the filtrate with ethyl acetate, drying the extract over sodium sulphate, concentration and crystallization of the residue with the addition of ether there can be obtained a further portion of 3,4-dimethoxy--5-nitroaniline. c) 10 g of finely powdered 3,4-diraethoxy-5-nitroaniline 15 are suspended in 15 ml of 12N hydrochloric acid and 40 ml of water, whereupon the suspension is stirring at 30° for 1 hour. Thereupon, 3.8 g of sodium nitrite dissolved in 20 ml of water are added dropwise thereto at -5° within 15 minutes. The solution is stirred at -5° for 30 minutes and 20 the cold diazoniura salt solution is added dropwise within 45 minutes to 100 ml of pyridine of 40°. The mixture is subsequently stirred at 70° for 1 hour. The reaction mixture is evaporated and the residue is taken up in 300 ml of ethyl acetate. It is extracted three times with 25 200 ml of 2N hydrochloric acid each time. The acidic- -aqueous phase is adjusted to pH 9 with conc. ammonia and extracted with methylene chloride. The combined methylene chloride extracts are dried over sodium sulphate and evaporated, and the residue is chromatographed on 250 g of 30 silica gel with ethyl acetate. After crystallization from methylene chloride/hexane there is obtained 2-(3.4--dimethoxy-5-nitrophenyl)pyridine of m.p. 89-90°. d) 1.5 g of 2-(3,4-dimethoxy-5-nitrophenyl)pyridine are 35 dissolved in 30 ml of 48 percent aqueous hydrobromic acid.
The reaction mixture is stirred at 100° for 16 hours and at 23° for 18 hours. The precipitate which thereby forms :WM:'^ C:': #1 0 h HQ A dL» y # ""-■ is filtered off and recrystallized from methanol/ether. There is obtained 3-nitro-5-(2-pyridyl)pyrocatechol hydrobromide of m.p. 239-240°.
Example 47 a) 2.76 ml of 2-bromopyridine dissolved in 30 ml of absolute tetrahydrofuran are treated -60° within 30 minutes with 19.2 ml of 1.6M n-butyl lithium solution (in hexane). whereupon the mixture is stirred at -60° for 30 minutes. 6.0 g of 3.4-dimethoxy-5-nitrobenzaldehyde dissolved in 50 ml of tetrahydrofuran are then added dropwise thereto at -40° within 30 minutes. The reaction 15 mixture is warmed to 0° within 2 hours, poured into ice-water and extracted with ethyl acetate. The combined extracts are dried over sodium sulphate and evaporated, and the residue is chromatographed on 200 g of silica gel with ethyl acetate. There is obtained a-(3,4-dimethoxy-20 -5-nitrophenyl)-2-pyridinemethanol as a brown oil. b) 7 g of manganese dioxide and added portionwise to 4.0 g of a-(3.4-dimethoxy-5-nitrophenyl)-2-pyridine-methanol dissolved in 100 ml of acetone while constantly heating under reflux over a period of 2.5 hours.
Thereupon, the mixture is heated under reflux for a further 2 hours. The manganese salts are subsequently filtered off and the residue obtained after evaporation is recrystallized from ether/hexane. There is obtained 30 3.4-dimethoxy-5-ni trophenyl 2-pyridyl ketone of m.p. 113°. c) 1.5 g of 3,4-dimethoxy-5-nitrophenyl-2-pyridyl ketone dissolved in 30 ml of 48 percent hydrobromic acid are stirred at 100° for 18 hours. Thereupon, the reaction mixture is evaporated and the residue is recrystallized from acetonitrile/methanol. There is obtained 3 , 4-d ihydroxy-5-ni trophenyl 2-pyridyl ketone hydrobromide -7i- 219496 of m.p. 213°.
Example 48 O 5 a) 11.3 g of tin dichloride dihydrate are added to 2.25 g of 31,4'-dimethoxy-5'-nitroacetophenone dissolved in 50 ml of ethanol, whereupon the mixture is stirred at 75° for 30 minutes. Thereupon, the reaction mixture is poured on to 100 g of ice, neutralized with about 300 ml of saturated sodium hydrogen carbonate solution and treated with 150 ml of methylene chloride. The mixture is filtered and the methylene chloride phase is separated. This is dried over sodium sulphate and evaporated, and the residue is 15 recrystallized from ether/petroleum ether. There is obtained 5'-amino-3',4'-dimethoxyacetophenone of m.p. 63-65°. b) A solution of 5.2 g of sodium nitrite in 20 ml of water is added dropwise at 0° within 20 minutes to 14.0 g of 5'-amino-31,4'-dimethoxyacetophenone dissolved in 155 ml of IN hydrochloric acid. After stirring at -2° for 30 minutes the cold diazonium salt solution is added ^ dropwise within 30 minutes at 5-10° to a solution of 8.7 g of copper(I) cyanide and 5.45 g of potassium cyanide in 60 ml of water. After completion of the addition 200 ml of methylene chloride are added, and the reaction mixture is stirred at 23° for 3 hours and then filtered. The organic phase is separated, washed with water, dried over sodium 30 sulphate and evaporated. The residue is recrystallized from methylene chloride/petroleum ether. There is obtained 5'-cyano-31,4'-dimethoxyacetophenone of m.p. 125-126°. c) 3.75 g of aluminium powder and 28.5 g of iodine are 35 heated under reflux for 2 hours in 160 ml of absolute benzene. 3.0 g of 51-cyano-31,4•-dimethoxyacetophenone and 0.5 g of tetra-n-butylammonium iodide are then added at 2 1949 °. whereupon the mixture is heated under reflux for 1 hour. Thereupon, the mixture is treated at 20° with 50 g of ice and filtered. The residue is washed with ethyl 5 acetate. The phases are separated and the aqueous phase is extracted a further twice with ethyl acetate. The combined organic phases are washed with 20 percent sodium thio-sulphate solution, dried over sodium sulphate and evaporated. The thus-obtained residue is dissolved in 20 ml of 10 acetic anhydride and 0.5 ml of pyridine and stirred at 120° for 6 hours. The mixture is subsequently evaporated and the residue is partitioned between methylene chloride and ice-water. The methylene chloride phase is dried over sodium sulphate and evaporated, and the residue is 15 chromatographed on 100 g of silica gel with methylene chloride. After recrystallization from ether there is obtained 5'-cyano-31,4'-diacetoxyacetophenone of m.p. 76-79°.
Example 49 0.33 g of 5'-cyano-31.4'-diacetoxyacetophenone dissolved in 3.3 ml of methanol is treated with 2.7 ml of 1.0N sodium hydroxide solution and the reaction mixture is 25 stirred at 23° for 45 minutes. The mixture is subsequently acidified with 2N hydrochloric acid, diluted with 5 ml of saturated sodium chloride solution and extracted twice with 30 ml of ethyl acetate each time. The combined ethyl acetate phases are dried over sodium sulphate and evapor-30 ated. The residue is recrystallized from toluene/aceto- nitrile. There is obtained 5'-cyano-3',4'-dihydroxyaceto-phenone as brownish crystals which decompose above 215°.
Example 50 a) 3.48 g of phenyltrimethylammonium bromide dibromide dissolved in 30 ml of tetrahydrofuran are added dropwise at room temperature within 45 minutes to 1.9 g of 5'-cyano-3'.4'-dimethoxyacetophenone dissolved in 30 ml of tetrahydrofuran. whereupon the mixture is stirred for 30 minutes. Thereupon, the reaction mixture is poured into 120 ml of ice-water and extracted three times with 70 ml of methylene chloride. The combined methylene chloride phases are washed with 2N sulphuric acid, dried over sodium sulphate and evaporated. The residue is chromato-graphed on 20 g of silica gel with methylene chloride. After recrystallization from methylene chloride/hexane there is obtained 5-(bromoacetyl)-2,3-dimethoxybenzo-nitrile of m.p. 138-141°. b) 1.45 g of 5-(bromoacetyl)-2.3-dimethoxybenzonitrile and 1.12 g of selenium dioxide are stirred at 120° for 18 hours in 10 ml of n-hexanol. After cooling to room temperature the mixture is diluted with 20 ml of methylene chloride and filtered. The filtrate is washed with water, dried over sodium sulphate and evaporated. The residue is chromatographed on 70 g of silica gel with hexane/ether (2:1). There is obtained hexyl (3-cyano-4.5-dimethoxy-phenyl)glyoxylate as an oil. c) 4.8 ml of boron tribromide dissolved in 20 ml of methylene chloride are added dropwise while cooling with ice within 20 minutes to 4.0 g of hexyl (3-cyano-4.5--dimethoxyphenyl)glyoxylate dissolved in 100 ml of methylene chloride and the reaction mixture is stirred at room temperature for 18 hours. 40 ml of methanol are subsequently added dropwise thereto at -60°, the mixture is stirred at room temperature for 1 hour and evaporated. The residue is taken up in methanol. It is heated under reflux for 10 minutes, evaporated to dryness and dried in a high vacuum. The thus-obtained crude product is recrystallized from acetonitrile. There is obtained methyl (3-cyano-4.5-dihydroxyphenyl)glyoxylate of m.p. 252°. 2 1949 — Example 51 1.075 g of methyl (3-cyano-4,5-dihydcoxyphenyl)-glyoxylate and 0.60 g of 2-amino-p-cresol ace stirred at 120° for 70 minutes in 2 ml of dimethylformamide. The mixture is subsequently cooled to room temperature and diluted with 15 ml of water. The precipitate is filtered off and dried in a water-jet vacuum at 80° for 6 hours. After recrystallization from acetonitrile there is obtained 2.3-dihydroxy-5-(6-methyl-2-oxo-2H-l,4--benzoxazin-3-yl)benzonitrile of m.p. 278-280°.
Example 52 a) A solution of 2.5 g (10.2 mmol) of 3.4-dimethoxy-5--nitrobenzoyl chloride in 10 ml of absolute tetrahydrofuran is treated with l.l ml of ethyl isocyanoacetate and subsequently with a solution of 3.0 ml of triethylamine in 10 ml of tetrahydrofuran and stirred at room temperature for 48 hours. After distillation of the solvent the residue is extracted with ethyl acetate/water. The crude product obtained after evaporation is chromatographed on a 20-fold amount of silica gel with ethyl acetate. After recrystallization from ethyl acetate/hexane there is obtained ethyl 5-(3,4-dimethoxy-5-nitrophenyl)-4-oxazole-carboxylate in the form of yellow crystals of m.p. 109-110°. b) 1.0 g (3.1 mmol) of ethyl 5-(3.4-dimethoxy-5-nitro-phenyl)-4-oxazolecarboxylate is treated with 10 ml of constant-boiling hydrobromic acid and stirred at 140° for 2 hours. After distillation of the excess hydrobromic acid the yellow residue is recrystallized from ethanol/acetone. There is obtained 2-amino-31.4'-dihydroxy-51-nitroacetophenone hydrobromide in the form of yellow crystals of m.p. >250° (dec. ). 2 19 4- 9 6 - 75 -Example 53 a) 10 g (34 mmol) of ethyl (3.4-dimethoxy-5-nitro-benzoyl)acetate are suspended in 100 ml of ethanol, treated with 1.7 g (37 mmol) of methylhydrazine and heated under reflux for 16 hours. After distillation of about 50 ml of ethanol the mixture is cooled to 0° and the separated precipitate is filtered off under suction. After recrystallization from ethanol there is obtained 3-(3,4--dimethoxy-5-nitrophenyl)-l-methylpyrazol-5-ol in the form of yellow crystals of m.p. 200-202°. b) 2.0 g (7.2 mmol) of 3-(3,4-dimethoxy-5-nitrophenyl)-1--methylpyrazol-5-ol are suspended in 100 ml of methylene chloride. After cooling to -40° a solution of 4.9 ml of boron tribromide in 60 ml of methylene chloride is added dropwise thereto within one hour. The mixture is subsequently stirred at room temperature for 16 hours, cooled to -20° and treated within 30 minutes with 100 ml of ethanol. After stirring at room temperature for one hour the solvent is distilled off in a water-jet vacuum at 40°. The residue is treated three times with a mixture of 100 ml of ethanol/toluene each time, with the solvent being distilled off each time. The residue is recrystallized from ethanol. There is obtained -(5-hydroxy-1-methy1- pyrazo1-3-yl)-3-nitropyrocatechol hydrobromide in the form of yellow crystals of m.p. >250°.
Example 54 a) 16.8 g (0.7 g-atom) of magnesium are treated with 15 ml of ethanol and. after adding 2 ml of carbon tetrachloride, the reaction is initiated by heating. A solution of 130.3 g of tert.butyl ethyl malonate in 70 ml of ethanol and 600 ml of absolute ether is added dropwise thereto while stirring within about 30 minutes so that the 2 19-49 reaction proceeds at the reflux temperature. The mixture is subsequently stirred at 50° for a further 3 hours and the solvent is distilled off at 40° in a water-jet vacuum. The residue is dissolved in 900 ml of tetrahydrofuran. To this solution is added dropwise while stirring at 50° a solution of 170 g (0.7 mol) of 3.4-dimethoxy-5-nitro-benzoyl chloride in 700 ml of absolute tetrahydrofuran and the mixture is stirred at the reflux temperature for one hour. The solvent is distilled off at 40° in a water-jet vacuum. The residue is treated with 1 1 of ether. 260 ml of 3N sulphuric acid are added thereto while cooling and stirring and the mixture is stirred for 30 minutes. The aqueous phase is extracted twice with 600 ml of ether each time. The organic phase is washed neutral with water, dried over sodium sulphate and evaporated. The brown oil obtained is filtered over 1 leg of silica gel with toluene. The resulting mixture, consisting of ethyl tert.butyl (3.4-dimethoxy-5-nitrobenzoyl)malonate and ethyl (3,4-diraethoxy-5-nitrobenzoyl)acetate, is dissolved in 600 ml of methylene chloride and treated while stirring within about 30 minutes with 193 ml of trifluoroacetic acid. The mixture is subsequently stirred at 40° for 2 hours and then evaporated at 40° in a water-jet vacuum. The oil obtained is extracted with ether/water. The organic phase is dried over sodium sulphate and evaporated. After dissolution in diisopropyl ether/hexane and cooling the separated crystals are filtered off under suction and recrystallized from diisopropyl ether. There is obtained ethyl (3,4-dimethoxy-5-nitrobenzoyl)acetate in the form of slightly yellowish crystals of m.p. 67-68°. i b) 10.0 g (33.6 mmol) of ethyl (3,4-dimethoxy-5-nitro-benzoyl)acetate are reacted with 4.0 g (37 mmol) of phenylhydrazine in analogy to Example 53a. After recrystallization from methylene chloride/ethanol there is obtained 3-(3.4-dimethoxy-5-nitrophenyl)-l-phenyl-2- -pyrazolin-5-one in the form of yellow crystals of m.p. 190-192°. c) In analogy to Example 53b, with boron tribromide there is obtained therefrom 5-(5-hydroxy-l-phenylpyrazol-3-yl)--3-nitropyrocatechol hydrobromide in the form of yellow crystals of m.p. >220° (dec.).
Example 55 .1 g of diethyl (3,4-dimethoxy-5-nitrobenzoyl)-malonate are dissolved in 200 ml of methylene chloride, whereupon the solution is cooled to -20° and at this 15 temperature there is added dropwise while stirring within 15 minutes a solution of 68.1 g of boron tribromide in 120 ml of methylene chloride. The mixture is subsequently stirred at room temperature overnight. After cooling to -20° the mixture is treated with 300 ml of ethanol and 20 stirred at room temperature for 30 minutes. The solvent is distilled off in a water-jet vacuum at 40°. The residue is treated with 300 ml of ice-water and methylene chloride. The organic phase is washed with water, dried over sodium sulphate and evaporated. The crude product obtained is 25 chromatographed on a 10-fold amount of silica gel with toluene. After crystallization from methylene chloride/ hexane there is obtained ethyl (3.4-dihydroxy-5-nitro-benzoyl)acetate in the form of yellow crystals of m.p. 136-137°.
Example 56 2.0 g (7.4 mmol) of ethyl (3.4-dihydroxy-5-nitro-benzoyl)acetate are suspended in 50 ml of ethanol. After 35 treatment with 0.4 g of hydrazine hydrate the mixture is held at the reflux temperature for 16 hours. After distillation of the solvent the residue is held at the 7 19^96 boiling temperature for 5 minutes with 50 ml of ethyl acetate. The separated precipitate is filtered off under suction and the filtrate is concentrated to 10 ml. The crystals, which separate in the cold, are filtered off under suction. There is obtained 5-(5-hydroxypyrazol-3--yl)-3-nitropyrocatechol in form of orange crystals of ra.p. 228° (dec.).
Example 57 7.3 g (36.66 mmol) of 3,4-dihydroxy-5-nitrobenzoic acid are treated with 30 ml of acetic anhydride, whereupon the mixture is held at the reflux temperature for 8 hours. The reaction mixture is poured on to ice. The separated precipitate is filtered off under suction, washed with water and taken up in methylene chloride. The organic phase is dried over sodium sulphate and evaporated. The residue obtained is recrystallized from methylene chloride/n-hexane in the cold. There is obtained 3,4-diacetoxy-5-nitrobenzoic acid in the form of colourless crystals of m.p. 126-127°.
Example 58 a) 9.7 g (32.2 mmol) of 3.4-diacetoxy-5-nitrobenzoic acid are treated with 12.5 ml of thionyl chloride, whereupon the mixture is stirred at 100° for 1.5 hours. After distillation of the excess thionyl chloride the 5-(chloro-carbonyl)-2-nitro-o-phenylene diacetate is distilled, b.p. 160° (26.7 Pa). b) 3.2 g (10.6 mmol) of 5-(chlorocarbony1)-2-nitro-o--phenylene diacetate are dissolved in 50 ml of dimethyl-formamide. The ice-cold solution is treated while stirring with a solution of 2.2 ml of diethylamine in 20 ml of dimethylformamide. The mixture is subsequently stirred at -J ^ ( / ' v., 30 2 194 room temperature for one hour, whereupon the solvent is distilled off in a water-jet vacuum at 50°. The residue obtained is treated with water and methylene chloride. The 5 organic phase is dried over sodium sulphate and evaporated. The yellow resin obtained is crystallized from methylene chloride/ether. There is obtained N.N-diethyl--3,4-dihydroxy-5-nitrobenzamide in the form of yellow crystals of m.p. 145-146°.
Example 59 3.2 g (10.6 mmol) of 5-(chlorocarbonyl)-2-nitro-o--phenylenediacetate are dissolved in 50 ml of diraethyl-15 formamide, whereupon the solution is treated at 0-5° while stirring and within 40 minutes with a solution of 3.02 ml of 2.2-diethylaminoethylamine in 20 ml of diraethyl-formamide. The mixture is subsequently stirred at room temperature for 30 minutes. The solvent is distilled off 20 in a water-jet vacuum at 60°. The residue is extracted twice with 20 ml of ethanol each time, dissolved in hot ethanol and treated with an excess of ethanolic hydrochloric acid, whereupon the mixture is evaporated. After recrystallization from ethanol/ethyl acetate there 25 is obtained N-[2-(diethylamino)ethyl]-3,4-dihydroxy-5- -nitrobenzamide hydrochloride in the form of yellow crystals of m.p. 139° (dec.).
Example 60 a) 10.0 g (47.4 mmol) of 2,3-dimethoxy-5-nitrobenz-aldehyde are treated with 50 ml of glacial acetic acid and 50 ml of constant-boiling hydrobromic acid and held at the reflux temperature for 7 hours. After treatment with ice 35 the separated precipitate is filtered off under suction, washed with water and taken up in ethyl acetate. The organic phase is dried over sodium sulphate and evapor- ated. The crude product obtained is filtered over silica gel with ethyl acetate. After crystallization from ethyl acetate/hexane there is obtained 2,3-dihydroxy-5-nitro-5 benzaldehyde in the form of brownish crystals of m.p. 226-228° . b) 4.5 g of 2,3-dihydroxy-5-nitrobenzaldehyde are suspended in 75 ml of water. After treatment with 4.2 g of 10 hydroxylamine o-sulphonic acid the mixture is stirred at 65° for 16 hours. After cooling the separated crystals are filtered off under suction and washed with water. The filtrate is extracted with ethyl acetate. The crystals and the dried organic phase are combined, whereupon the 15 mi-niire is evaporated and the residue is recrystallized frotn diisopropyl ether. There is obtained 2.3-dihydroxy-5--nitrobenzonitrile in the form of yellow crystals of m.p. 186-188° .
Example 61 a) 4.0 g (19.2 mmol) of 3.4-diraethoxy-5-nitro-benzo-nitrile are dissolved in 50 ml of dimethylformamide, whereupon the solution is treated with 1.66 g of ammonium chloride and 2,02 g of sodium azide and stirred at 125° for 31 hours. After in each case 8 and 15 hours the same amounts of ammonium chloride and sodium azide are added thereto. After cooling the mixture is poured on to ice. The separated precipitate is filtered off under suction, 30 washed with water and dried. There is obtained 2-methoxy--6-nitro-4-(lH-tetrazol-5-yl)phenol in the form of orange crystals of m.p. >240° (dec.). b) 4.0 g (16.9 mmol) of 2-methoxy-6-nitro-4-(lH-tetrazol-35 -5-yl)phenol are treated with 40 ml of constant-boiling hydrobromic acid, whereupon the mixture is stirred at 140° for 8 hours under a nitrogen atmostphere. After cooling n 2 the mixture is poured on to ice. The separated precipitate is filtered off under suction and recrystallized from ether. There is obtained 3-nitro-5-(lH-tetrazol-5-yl)-5 pyrocatechol in the form of orange crystals of m.p. >240° (dec.).
Example 62 ^ . 10 A total of 7.2 g (40 mmol) of 3.4-dihydroxy-5-nitro- v" benzonitrile are introduced portionwise into 130 ml of conc. sulphuric acid while stirring within 10 minutes, whereupon the mixture is stirred at 50° for 4 hours. The reaction mixture is poured into 800 ml of ice-water. The 15 separated precipitate is filtered off under suction, washed with water and taken up in ethyl acetate. The organic phase is dried over sodium sulphate and evaporated. After recrystallization from acetone/ethyl acetate there is obtained 3.4-dihydroxy-5-nitrobenzamide in the 20 form of orange crystals of m.p. 235-236°.
Example 63 a) A solution of 11.25 g (82.6 mmol) of 27hydroxyaceto-25 phenone in 100 ml of absolute dimethylformamide is added dropwise under an argon atmosphere within 15 minutes to a suspension of 3.6 g (82.5 mmol) of a 55 percent sodium hydride dispersion in 50 ml of absolute dimethylformamide and the mixture is stirred at room temperature for one vJ 30 hour. After cooling to 0° a solution of 20.3 g (82.6 mmol) of 3,4-dimethoxy-5-nitrobenzoyl chloride in 100 ml of absolute dimethylformamide is added dropwise thereto within 20 minutes and the mixture is sti;t..i temperature overnight. The reaction mixture is poured into 35 ice-waiter, whereupon the mixture is extracted twice with 250 ml of ethyl acetate each time. The organic phase is extracted twice with 100 ml of sodium chloride solution ISt:;f?^ m 2 A (T c each time, dried over sodium sulphate arid evaporated. The brown oil obtained is heated in 100 ml of toluene. The separated precipitate is filtered off under suction and the filtrate is chromatographed on a 30-fold amount of silica gel with toluene/ethyl acetate (4:1). After recrystallization from ethyl acetate/hexane there is obtained o-acetylphenyl 3,4-dimethoxy-5-nitrobenzoate in the form of yellowish crystals of m.p. 108-109°. b) 10.0 g (29 mmol) of o-acetylphenyl 3,4-dimethoxy-5--nitrobenzoate are dissolved in 50 ml of pyridine. After treatment with 8.12 g (144 mmol) of powdered and dried potassum hydroxide the mixture is stirred at 80° for 5 minutes. After cooling the mixture is poured on to ice. The aqueous solution is made acid by treatment with 3N hydrochloric acid. The separated precipitate is filtered off under suction. After recrystallization from ethyl acetate/hexane there is obtained l-(o-hydroxyphenyl)-3--(3,4-dimethoxy-5-nitrophenyl)-l.3-propanedione in the form of yellowish crystals of m.p. 188-189°. c) A solution of 1.82 g (0.7 ml) of boron tribromide in 20 ml of methylene chloride is added dropwise within about 25 20 minutes to a solution of 500 mg (1.45 mmol) of 1-(o-hydroxyphenyl)-3-(3,4-diraethoxy-5-nitrophenyl)-1.3--propanedione in 50 ml of methylene chloride while stirring and under an argon atmosphere at -20°, whereupon the mixture is stirred at room temperature overnight. 30 After cooling to -20° the mixture is treated dropwise with 25 ml of ethanol and evaporated at 40° in a water-jet vacuum. After recrystallization from ethanol there is obtained l-(3,4-dihydroxy-5-nitrophenyl)-3--(o-hydroxyphenyl)-1,3-propanedione in the form of yellow 35 crystals of m.p. 251-252°.
Example 64 a) A solution of 2.0 g (5.79 mmol) of o-acetylphenyl 3.4-dimethoxy-5-nitrobenzoate in 12.5 ml of glacial acetic acid is treated with 0.94 g of sodium acetate and held at the reflux temperature for 4 hours. After cooling the mixture is poured into ice-water. The separated crystals are filtered off under suction. After recrystallization from ethyl acetate/hexane there is obtained 2-(3.4--dimethoxy-5-nitrophenyl)-4H-l-benzopyran-4-one in the form of colourless crystals of m.p. 216-217°. b) A solution of 10 ml of boron tribromide in 50 ml of methylene chloride are added dropwise within 30 minutes at -10° to a solution of 1.0 g (2.9 mmol) of 2-(3,4--dimethoxy-5-nitrophenyl)-4H-l-benzopyran-4-one in 100 ml of methylene chloride under an argon atmosphere, whereupon the mixture is stirred at room temperature overnight.
After cooling to -20° 20 ml of ethanol are added dropwise thereto. The mixture is then evaporated in a water-jet vacuum. The yellow residue obtained is extracted with water/ethyl acetate. The organic phase is washed with water, dried over sodium sulphate and evaporated. After recrystallization from ethanol/ethyl acetate there is obtained 2-(3,4-dihydroxy-5-nitrophenyl)-4H-l-benzopyran--4-one in the form of yellow crystals of m.p. >240° (dec.).
Example 65 a) 92 ml of n-butyl lithium solution (1.6M in hexane) are added dropwise at -70° within 20 minutes to 33.0 g of 4-bromobenzotrifluoride (dissolved in 150 ml of tetrahydrofuran). After stirring at -70° for 45 minutes 36 g o£ 3-methoxy-4-benzyloxybenzaldehyde (dissolved in 100 ml of tetrahydrofuran) are added dropwise thereto at between -70° and -60°. The reaction mixture is stirred at -70° for 2 194 9 2 hours and at 0° for 1 hour, poured into a mixture of ice and 100 ml of 2N sulphuric acid and extracted twice with 500 ml of ether. The combined ether phases are washed with saturated sodium chloride solution, dried over sodium sulphate and evaporated. There is obtained 4-(benzyloxy)~ -3-methoxy-4'-(trifluoromethyl)benzhydrol which can be used directly in the subsequent reaction step. b) 52.6 g of 4-(benzyloxy)-3-methoxy-4'-(trifluoromethyl )benzhydrol (dissolved in 500 ml of methylene chloride) are treated within 10 minutes at 20° with 30.6 g of pyridinium chlorochromate and stirred at 20° for 2 hours. The precipitate formed is subsequently filtered off and washed with methylene chloride. The filtrate is evaporated and the residue is chromatographed on 150 g of silica gel with methylene chloride. After recrystallization from methylene chloride/hexane there is obtained 4-(benzyloxy)-3-methoxy-4'-(trifluoromethyl)benzophenone of melting point 101°. c) 70 ml of 33 percent hydrobromic acid in acetic acid are added within 15 minutes at 10° to 20 g of 4-(benzyloxy)-3-methoxy-41 -(trifluoromethyl)benzophenone (dissolved in 150 ml of methylene chloride). After stirring at 20° for 1.5 hours the reaction mixture is poured into 600 ml of ice-water; the methylene chloride phase is separated and the aqueous phase is extracted a further twice with 100 ml of methylene chloride. The combined methylene chloride phases are washed with 600 ml of water, dried over sodium sulphate and evaporated. The residue is chromatographed on 150 g of silica gel with methylene chloride. After recrystallization from methylene chloride/hexane there is obtained 4-hydroxy-3-methoxy-41 --(trifluoromethyl)benzophenone of melting point 97°. d) 3.2 ml of 65 percent nitric acid are added dropwise within 10 minutes at 20° to 12.8 g of 4-hydroxy-3-methoxy--4 *-(trifluoromethyl)benzophenone (dissolved in 160 ml of acetic acid). After stirring for 1.5 hours the reaction mixture is poured into 600 ml of ice-water, and the precipitate formed is filtered off, washed with water and dissolved in methylene chloride. The methylene chloride solution is dried over sodium sulphate and evaporated, and the residue is recrystallized from methylene chloride/ hexane. There is obtained 4-hydroxy-3-methoxy-5-nitro-4'--(trifluoromethyl)benzophenone of melting point 172°. e) 2.0 g of 4-hydroxy-3-raethoxy-5-nitro-4'-(trifluoro-methyl)benzophenone (dissolved in 20 ml of 33 percent hydrobromic acid in glacial acetic acid) are stirred at 90° for 18 hours. Thereupon, 20 ml of 48 percent aqueous hydrobromic acid are added thereto, whereupon the mixture is stirred at 110° for a further 18 hours. The reaction mixture is subsequently evaporated under reduced pressure and the residue is recrystallized from water. There is obtained 3,4-dihydroxy-5-nitro-4'-(trifluoromethyl)benzophenone of melting point 116-118°.
Example 66 a) 18.7 g of 4-hydroxy-3-methoxy-5-nitro-41 -(trifluoro-methyl)benzophenone (dissolved in 250 ml of tetrahydrofuran) are treated at room temperature with 27.5 ml of 2N potassium hydroxide solution, whereupon the mixture is evaporated. The residue is treated with 200 ml of toluene, whereupon the mixture is again evaporated. Thereupon, the mixture is heated with 400 ml of toluene for 4 hours with the separation of water and under reflux. 100 ml of toluene are distilled off and 10 ml of dimethylformamide and 20 ml of dimethyl sulphate (freshly distilled) are added, whereupon the mixture is heated under reflux for 5 hours. 300 ml of IN sodium hydroxide solution are # 2 101 a ^ subsequently added at 20°. The reaction mixture is stirred for 30 minutes and treated with 200 ml of ether. The organic phase is separated; the aqueous phase is extracted 5 a further twice with 100 ml of ether; the combined ether phases are dried over sodium sulphate and evaporated, and the residue is chromatographed on 70 g of silica gel with methylene chloride. After recrystallization from methylene chloride/hexane there is obtained 3.4-dimethoxy-5-nitro-(^) 10 -4'-(trifluoromethyl)benzophenone of melting point 115°. b) 49.5 g of tin dichloride dihydrate are added to 16.0 g of 3,4-diraethoxy-5-nitro-4'-(trifluoromethy1)benzophenone (dissolved in 300 ml of ethanol), whereupon the mixture is 15 stirred at 75° for 30 minutes. Thereupon, the reaction mixture is poured into 800 ml of ice-water. It is neutralized with 28 percent sodium hydroxide solution and extracted three times with 600 ml of methylene chloride. The combined methylene chloride phases are washed with 20 water, dried over sodium sulphate and evaporated. After recrystallization from methylene chloride/hexane there is obtained 5-amino-3.4-dimethoxy-41 -(tr ifluoromethyl)benzophenone of melting point 95-96°. i s~" 25 3.25 g of 5-amino-3.4-dimethoxy-41-(tritluoromethyl)- benzophenone (dissolved in 50 ml of acetone) are evaporated under reduced pressure after the addition of 15 ml of 2N sulphuric acid. The thus-obtained residue is suspended , in 20 ml of acetic acid, diluted with 100 ml of water and treated at 5° with a solution of 700 mg of sodium nitrite in 10 ml of water. It is stirred at 5° for one hour. Thereupon, the diazonium salt solution is filtered, added at 5° to a solution of 2.0 g of sodium cyanide and 1.0 g of copper(I) cyanide in 20 ml of water and stirred at 5° 35 for 1 hour. Thereupon. 200 ml of methylene chloride are added thereto. Insoluble constituents are filtered off. The phases are separated; the aqueous phase is extracted a . ...... ^ 2 4 ^ A -<$? ,:.. , K**$ tu' <*' & further twice with 100 ml of methylene chloride. The combined methylene chloride phases are washed with water, dried over sodium sulphate and evaporated. The residue is n 5 chromatographed on 30 g of silica gel with methylene chloride. After recrystallization from methylene chloride/hexane there is obtained 5-cyano-3,4-dimethoxy--4'-(trifluoromethyl)benzophenone of melting point 130°. d) 1.5 g of 5-cyano-3.4-dimethoxy-4'-(trifluoromethyl)-benzophenone (dissolved in 75 ml of methylene chloride) are treated at 5° with 2.18 ml of boron tribromide, whereupon the mixture is stirred at room temperature for 18 hours. The reaction mixture is subsequently diluted 15 with 50 ml of methylene chloride. The mixture is heated under reflux for a further 4 hours, treated at -70° with 15 ml of methanol, stirred at room temperature for 2 hours, evaporated, the residue is dried in vacuo and partitioned between ethyl acetate and ice-water. The 20 aqueous phase is extracted a further twice with ethyl acetate. The combined ethyl acetate phases are dried over sodium sulphate and evaporated. The thus-obtained material is stirred at 130° for 6 hours with 10 ml of acetic anhydride and 1 ml of pyridine. The mixture is evaporated 25 and the residue is partitioned between ice-water and methylene chloride. The methylene chloride phase is dried over sodium sulphate and evaporated, and the residue is chromatographed on 30 g of silica gel with methylene chloride. The thus-obtained diacetate is dissolved in 30 10 ml of methanol. The solution is treated with 4.2 ml of IN sodium hydroxide solution, stirred at 0° for 1 hour, neutralized with acetic acid, evaporated and partitioned between ethyl acetate and saturated sodium chloride solution. The ethyl acetate phases are dried over sodium 35 sulphate and evaporated, and the residue is recrystallized from methylene chloride. There is obtained 5-cyano-3,4--dihydroxy-4'-(trifluoromethyl)benzophenone of melting 2 88 - point 204-206' In an analogous manner: al) From 2'-fluoro-4-hydroxy-3-methoxy-5-nitrobenzophenone there is obtained 3.4-dimethoxy-21-fluoro-5-nitrobenzo-phenone of m.p. 86-88° (from ether/low-boiling petroleum ether). bl) from 3,4-dimethoxy-2'-fluoro-5-nitrobenzophenone there is obtained 5-amino-3,4-diraethoxy-21-fluorobenzophenone of m.p. 93-95° (from ether/low-boiling petroleum ether). cl) from 5-amino-3.4-dimethoxy-2'-fluorobenzophenone there is obtained 5-benzoyl-2.3-dimethoxy-2'-fluorobenzonitrile of m.p. 132-134° (from methylene chloride/low-boiling petroleum ether) and dl) from 5-benzoyl-2.3-dimethoxy-2'-fluorobenzonitrile there is obtained 5-benzoyl-2.3-dihydroxy-21-fluorobenzonitrile of m.p. 228-230° (from ether/low-boiling petroleum ether).
In an analogous manner: b2) from 3.4-dimethoxy-5-nitrobenzophenone there is obtained 5-amino-3.4-dimethoxybenzophenone as an amorphous solid, c2) from 5-amino-3.4-dimethoxybenzophenone there is obtained 5-benzoyl-2.3-dimethoxybenzonitrile of m.p. 98-100° (from ether/hexane) and d2) from 5-benzoyl-2,3-dimethoxybenzonitrile there is obtained 5-benzoyl-2,3-dihydroxybenzonitrile of m.p. 212-214° (from ethyl acetate/ether).
A 7 * 0 Example 67 a) A solution of 16.0 g (65.14 mmol) of 3,4-dimethoxy-5--nitrobenzoyl chloride in 80 ml of pyridine is added (H dropwise to a solution of 2.68 g (32.64 mmol) of 1-methyl- imidazole and 6.6 g (65.14 mmol) of triethylamine in 80 ml of pyridine, whereupon the mixture is stirred at 60° for 3 hours. After treatment with 1.70 ml of 3N sodium hydroxide solution the mixture is stirred for a further 1 hour and subsequently poured into ice-water. The separated grey crystals are filtered off under suction and taken up in ethyl acetate, whereupon the organic phase is dried over magnesium sulphate and the solvent is distilled off. After recrystallization from ethyl acetate/hexane there is obtained 3,4-dimethoxy-5-nitrophenyl (l-methylimidazol-2--yl) ketone in the form of colourless crystals of m.p. 144-145°. b) 5.0 g (17.17 mmol) of 3.4-dimethoxy-5-nitrophenyl (l-roethylimidazol-2-yl) ketone are treated with 50 ml of hydrobromic acid (48%). whereupon the mixture is stirred under reflux temperature for 2 hours. After cooling the separated precipitate is filtered off under suction, washed with ice-water and recrystallized from ethanol. There is obtained 3,4-dihydroxy-5-nitrophenyl (1-methyl-imidazol-2-yl) ketone hydrobromide in the form of yellow crystals of decomposition point >240°.
Example 68 In analogy to Example 67, from 3.4-dimethoxy-5-nitro-benzoyl chloride and 1-benzylimidazole there is obtained l-benzylimidazol-2-yl (3.4-dimethoxy-5-nitrophenyl) ketone in the form of colourless crystals of m.p. 134-135° (from methylene chloride/hexane) and therefrom with hydrogen bromide there is obtained l-benzylimidazol-2-yl -? Q A Q c 90 - (3.4-dihydroxy-5-nitrophenyl) ketone hydrobromide as yellow crystals of m.p. 218-219° (dec.).
Example 69 a) A solution of 10.0 g (53.13 mmol) of l-[(benzyloxy)-methyl]imidazole in 50 ml of acetonitrile is added dropwise within 10 minutes while cooling with ice to a solution of 13.0 g (53.13 mmol) of 3.4-dimethoxy-5-nitro-benzoyl chloride and 5.4 g (53.13 mmol) of triethylamine in 80 ml of acetonitrile so that the temperature does not exceed 25°. The mixture is subsequently stirred for a further 3 hours, whereupon the solvent is distilled off. After treatment with water the mixture is extracted with ethyl acetate. After two-fold washing with water the organic phase is dried over sodium sulphate and evaporated. The resulting oil is chromatographed on 600 g of silica gel with toluene/ethyl acetate (95:5). There is obtained 1-[(benzyloxy)methyl]imidazo1-2-yl (3.4-dimethoxy-5-nitrophenyl) ketone as a yellowish oil. b) In analogy to Example 67b) there is obtained after treatment with hydrobromic acid 3.4-dihydroxy-5-nitro-phenyl (imidazol-2-yl) ketone hydrobromide as yellow crystals of m.p. 247-248°.
Example 70 a) A solution of 25.0 g (120.16 mmol) of 3-bromoquinoline is dissolved in 200 ml of dry ether and cooled to -60°. At this temperature there are added dropwise within 15 minutes 75.1 ml (120.16 mmol) of a 1.6 molar solution of n-butyllithium. whereupon the mixture is stirred for 10 minutes. A solution of 26.5 g (109.2 mmol) of vanillin benzyl ether in 250 ml of dry ether is added dropwise thereto at -60°, the mixture is subsequently stirred at m rr 10 2 ..4 room temperature for 3 hours, poured into about 1.5 1 of ice-water and extracted three times with 600 ml of ethyl acetate each time. The organic phase is washed with water, dried over sodium sulphate and evaporated. The resulting oil is chromatographed on 1 kg of silica gel with methylene chloride/ethyl acetate (1:1). The crystalline crude product obtained is recrystallized from ethyl acetate. There is obtained a-[4-(benzyloxy)-3-methoxy-phenyl]-3-quinolinemethanol in the form of colourless crystals of m.p. 124-125°. b) A solution of 6.2 g (16.7 mmol) of a-[4-(benzyloxy)--3-methoxyphenyl]-3-quinolinemethanol in 200 ml of methylene chloride is treated with 62 g of manganese dioxide and stirred at the reflux temperature 2 hours. After cooling the precipitate is filtered off under suction and washed with methylene chloride. The filtrate is evaporated and the oil obtained is dissolved in hot ether, whereupon the solution is treated with a small amount of pentane. The separated crystals are filtered off under suction. There is obtained 4-(benzyloxy)-3-methoxy-phenyl (3-quinolinyl) ketone in the form of colourless crystals of m.p. 110-111°. c) 11.0 g (29.78 mmol) of 4-(benzyloxy)-3-methoxyphenyl (3-quinolinyl) ketone are treated with 50 ml of trifluoroacetic acid, whereupon the mixture is stirred at room temperature for 2 hours. After distillation of the trifluoroacetic acid the residue is treated twice with 50 ml of ethanol each time and the solvent is distilled off each time. The oil obtained crystallizes upon treatment with ethanol. After recrystallisation from ethanol there is obtained 4-hydroxy-3-methoxyphenyl (3-quinolinyl) ketone in the form of yellowish crystals of m.p. 196-197°. d) A solution of 1.91 ml of 65 percent nitric acid in m n ml of glacial acetic acid is added dropwise to a solution of 5.5 g (19.69 mmol) of 4-hydroxy-3-methoxy-phenyl (3-quinolinyl) ketone in 300 ml of glacial acetic 5 acid at 150° and the mixture is then stirred at this temperature for a further 2 hours. The mixture is then poured into ice-water and extracted three times with 300 ml of ethyl acetate each time. The organic phase is washed five times with 100 ml of water each time, dried 10 over sodium sulphate and evaporated. After treatment of the residue with ethyl acetate there is obtained 4-hydroxy-3-methoxy-5-nitrophenyl (3-quinolinyl) ketone in the form of yellow crystals of m.p. 220-221° (dec.). e) 820 mg (2.53 mmol) of 4-hydroxy-3-methoxy-5-nitro-phenyl (3-quinolinyl) ketone are treated with 50 ml of 48 percent hydrobromic acid and held at the reflux temperature for 3 hours. After distillation of the hydrobromic acid at 50° the residue is treated with 70 ml of hot water and the insoluble constituent is filtered off under suction. There is obtained 3,4-dihydroxy-5-nitrophenyl (3-quinolinyl) ketone hydrobromide in the form of yellow crystals of m.p. 270° (dec.).
Example 71 In analogy to Example 70 there is obtained a-[4-(benzyloxy)-3-methoxyphenyl]-4-quinolinemethanol as colourless crystals of m.p. 117-118° (ethyl acetate), therefrom with manganese dioxide there is obtained 4-(benzyloxy)-3-methoxyphenyl (4-isoquinolinyl) ketone as colourless crystals of m.p. 126.5-127.5°, therefrom with trifluoroacetic acid there is obtained 4-hydroxy-3--methoxyphenyl (4-isoquinolinyl) ketone as yellow crystals of m.p. 197.5-198.5° and therefrom by nitration with nitric acid in glacial acetic acid and subsequent treatment with hydrobromic acid there is obtained _ „ _ 2 194 3,4-dihydroxy-5-nitrophenyl (4-isoquinolinyl) ketone hydrobromide as yellow crystals of m.p. 256° (dec.).
Example 72 In analogy to Example 70 there is obtained a-[4-(benzyloxy)-3-methoxyphenyl]-2-naphthalenemethanol as colourless crystals (ethyl acetate/hexane) of m.p. 113-114°. therefrom with manganese dioxide there is obtained 4-(benzyloxy)-3-methoxyphenyl (2-naphthyl) ketone as colourless crystals (ethyl acetate/hexane) of m.p. 104-105°. therefrom by treatment with trifluoroacetic acid and nitration with nitric acid in glacial acetic acid there is obtained 4-hydroxy-3-methoxy-5-nitrophenyl (2-naphthyl) ketone as yellow crystals of m.p. 187-188° and therefrom by treatment with hydrobromic acid there is obtained 3.4-dihydroxy-5-nitrophenyl (2-naphthyl) ketone as yellow crystals of m.p. 184-185°.
Example 73 a) A solution of 20.0 g (100.5 mmol) of 3-phenylpropyl bromide in 300 ml of ether is treated at -60° while stirring within 15 minutes with a solution of 71.8 ml (100.5 mmol) of tert.butyllithium (1.4 molar) in pentane. After 10 minutes there is added dropwise at this temperature within 15 minutes a solution of 22.14 g (91.36 mmol) of vanillin benzyl ether in 200 ml of ether, whereupon the mixture is stirred for a further 2 hours. The mixture is poured into 1 1 of ice-water and extracted three times with 500 ml of ethyl acetate each time. The organic phase is washed twice with 200 ml of water each time, dried over sodium sulphate and evaporated. The oil obtained is chromatographed on 1 kg of silica gel with methylene chloride. The crystals obtained are recrystallized from ether/pentane. There is obtained 4-(benzyloxy)-3-methoxy- »-iVaifc*.- •. •" ■,v •-.
H o "1 2 >y >—st» -a-(3-phenylpropyl)benzyl alcohol in the form of colourless crystals of m.p. 71-73°. b) A solution of 9.0 g (24.83 mmol) of 4-(benzyloxy)-3--methoxy-a-(3-phenylpropyl)benzyl alcohol in 250 ml of methylene chloride is treated with 90 g of manganese dioxide and held at the reflux temperature for 2 hours. After cooling the precipitate is filtered off under suction and washed with methylene chloride. The filtrate is evaporated and the residue is recrystallized from ethyl acetate/ether. There is obtained 4'-(benzyloxy)-3•--methoxy-4-phenylbutyrophenone in the form of colourless crystals of m.p. 81-82°. c) A solution of 7.0 g (19.42 mmol) of 4'-(benzyloxy)-31--methoxy-4-phenylbutyrophenone in 40 ml of 33 percent hydrobromic acid in glacial acetic acid is stirred at room temperature for 5 hours and subsequently poured into 500 ml of ice-water. The solution is adjusted to pH 6.0 by the addition of conc. ammonia and extracted three times with 250 ml of ethyl acetate each time. The organic phase is washed three times with 50 ml of water each time, dried over sodium sulphate and evaporated. The oil obtained is dissolved in 20 ml of ether, whereupon the solution is treated with hexane until it becomes turbid and is left to crystallize out. There is obtained colourless 4'-hydroxy--3'-methoxy-4-phenylbutyrophenone of m.p. 91-92°. d) A solution of 0.79 ml of 65 percent nitric acid in 25 ml of glacial acetic acid is added dropwise to a solution of 2.2 g (8.14 mmol) of 4'-hydroxy-31-methoxy-4--phenylbutyrophenone in 25 ml of glacial acetic acid and the mixture is stirred at room temperature for a further 2 hours. The mixture is poured into 150 ml of ice-water and. after treatment with 20 ml of 3N hydrochloric acid, extracted three times with 75 ml of ethyl acetate each i i 95 - 2 1949 time. The organic phase is washed with water, dried over sodium sulphate and evaporated. The crude product obtained is taken up in ethyl acetate and filtered over 75 g of silica gel. After recrystallization from acetonitrile there is obtained 41-hydroxy-3'-methoxy-51-nitro-4-phenyl-butyrophenone in the form of yellow crystals of m.p. 120-121°. e) 1.0 g (3.2 mmol) of 4•-hydroxy-3'-methoxy-5'-nitro-4--phenylbutyrophenone is held at 200° for 1 hour with 8 g of pyridine hydrochloride. The reaction mixture is poured while still warm into ice-water and extracted with ethyl acetate. The organic phase is washed with IN hydrochloric acid and subsequently with water, dried over sodium sulphate and evaporated. The dark residue obtained is chromatographed on a 30-fold amount of silica gel with ethyl acetate. From methylene chloride/hexane there is obtained 3'.4•-dihydroxy-5'-nitro-4-phenylbutyrophenone in the form of yellow crystals of m.p. 118-119°.
Example 74 a) A solution of 14.68 g (225.4 mmol) of potassium cyanide in 20 ml of water is added to a solution of 10.0 g (47.4 mmol) of 3,4-dimethoxy-5-nitrobenzaldehyde in 100 ml of dioxan. 18.81 ml (190.76 mmol) of 37 percent hydrochloric acid are now added dropwise thereto within 30 minutes while stirring vigourously. After the addition of 120 ml of ether the excess hydrogen cyanide gas is driven off by passing argon through the mixture. The reaction mixture is filtered over a siliceous earth filter aid, and the organic phase is washed with water dried over sodium sulphate and evaporated. The a-hydroxy-3.4-dimethoxy-phenylacetonitrile (yellowish oil) which is formed is dissolved in 200 ml of ether, whereupon the solution is treated with 20 ml of ethanol, cooled to 0° and hydrochloric acid gas is passed in for 30 minutes. After 3 hours the separated colourless precipitate is filtered off under suction and recrystallized from ethanol/ether. There is obtained ethyl (3,4-dimethoxy-5-nitrophenyl)hydroxy-acetimidate hydrochloride. b) 19.7 g (61.46 mmol) of ethyl (3,4-dimethoxy-5-nitro-phenyl)hydroxyacetiraidate are dissolved in 500 ml of ethanol, 6.73 g (61.46 mmol) of o-phenylenediamine are added, the mixture is stirred at room temperature for 2 hours and subsequently held at the reflux temperature overnight. After distillation of the solvent the residue is treated with 50 ml of water, made alkaline with sodium carbonate solution and extracted twice with 250 ml of methylene chloride each time. The organic phase is washed with water, dried over sodium sulphate and evaporated. The orange residue obtained is chromatographed on 400 g of silica gel with methylene chloride/ethyl acetate (1:1). From ether/hexane there is obtained a-(3.4-dimethoxy-5--nitrophenyl)-2-benzimidazolemethanol in the form of yellowish crystals of m.p. 50° (dec.). c) 13.2 g (40.1 mmol) of a-(3.4-dimethoxy-5-nitro-phenyl)-2-benzimidazolemethanol are dissolved in 200 ml of methylene chloride and. after treatment with 130 g of manganese dioxide, the mixture is stirred at the reflux temperature for 2 hours. After filtration the solvent is distilled off. There is obtained 2-benzimidazolyl (3,4-dimethoxy-5-nitrophenyl) ketone in the form of yellowish crystals of m.p. 212-213°. d) 1.0 g (3.05 mmol) of 2-benzimidazolyl (3.4-dimethoxy--5-nitrophenyl) ketone and 8.0 g of pyridine hydrochloride are held at 200° for 60 minutes. The dark solution is poured while still warm into ice-water and extracted three times with 100 ml of ethyl acetate each time. The organic phase is washed with water, dried over sodium sulphate and evaporated. After recrystallization from ethyl acetate/ hexane there is obtained 2-benzimidazolyl (3,4-dihydroxy--5-nitrophenyl) ketone in the form of yellow crystals of m.p. 249-250°.
Example 75 a) 30.0 g (132 mmol) of 3.4-dimethoxy-5-nitrobenzoic acid are dissolved in 250 ml of tetrahydrofuran and, after the addition of 21.85 g (135 mmol) of 1,1'-carbonyl-diiraidazole, the mixture is stirred at the reflux temperature for 2 hours. The mixture is poured into 300 ml of ice-water and the precipitated crystals are filtered off under suction after stirring for 30 minutes. The crystals are taken up in methylene chloride, whereupon the organic phase is washed with water, dried over sodium sulphate and evaporated. After crystallization from methylene chloride/hexane there is obtained 1-(3,4-dimethoxy-5--nitrobenzoyl)imidazole in the form of colourless crystals of m.p. 136-137°. b) 10.0 g (36.1 mmol) of l-(3,4-dimethoxy-5-nitro-benzoyl)imidazole in 50 ml of dimethylformamide are treated with 6.95 g (93.8 mmol) of acetamidoxime, whereupon the mixture is stirred at 70° for 1 hour. After cooling the mixture is poured into 500 ml of ice-water and stirred for 30 minutes. The separated crystals are filtered off under suction and washed with water. After crystallization from ethyl acetate there is obtained N1-[(3,4-dimethoxy-5-nitrobenzoyl)oxy]acetamidine in the form of colourless crystals of m.p. 165-166°. c) 2.0 g (1.2 mmol) of N1-[(3,4-dimethoxy-5-nitro-benzoyl)oxyjacetamidine are held at reflux temperature for 1 hour in 20 ml of glacial acetic acid. After distillation 2 19496 - 98 - — ^ of the acetic acid the crystalline residue is recrystallized from ether/hexane. There is obtained 5-(3,4--dimethoxy-5-nitrophenyl)-3-methyl-l.2,4-oxadiazole in the form of colourless crystals of m.p. 111°. d) 2.5 g (9.43 mmol) of 5-(3.4-dimethoxy-5-nitrophenyl)--3-methyl-l,2,4-oxadiazole are dissolved in 70 ml of methylene chloride. After cooling to -60° there is added dropwise thereto within 20 minutes while stirring a solution of 23.62 g (94.3 mmol) of boron tribromide in 50 ml of methylene chloride, whereupon the mixture is held at the reflux temperature for 48 hours. After cooling to -60° the mixture is treated with 60 ml of ethanol and subsequently stirred at room temperature for 30 minutes. The yellow solution is evaporated to dryness, whereupon the residue is treated three times with 100 ml of toluene/ethanol (1:1) each time and the solvent is distilled off each time. After crystallization from ethanol there is obtained 5-(3-methyl-l,2,4-oxadiazol-5--yl)-3-nitropyrocatechol in the form of yellow crystals of m.p. 201-202°.
Example 76 a) 143.8 ml of n-butyllithium solution (1.53M in hexane) are added dropwise at -70° within 30 minutes to 35.0 g of l-bromo-2-fluorobenzene (dissolved in 600 ml of tetrahydrofuran). After stirring at -70° for 60 minutes 48.5 g of 3-methoxy-4-benzyloxybenzaldehyde (dissolved in 450 ml of tetrahydrofuran) are added dcopwise thereto during 30 minutes. The reaction mixture is stirred at -70° for 2 hours and at 0° for 30 minutes, poured into a mixture of ice and 150 ml of 2N sulphuric acid and extracted three times with 500 ml of ether. The combined ether phases are washed with saturated sodium chloride solution, dried over sodium sulphate and evaporated. There is obtained 4-(benzyloxy)-2'-fluoro-3'-raethoxybenzhydro1 as a yellowish oil which can be used directly in the subsequent reaction step.
In an analogous manner: al) From 3-methoxy-4-benzyloxybenzaldehyde and l-bromo-3--fluorobenzene there is obtained 4-(benzyloxy)-3'--fluoro-3-methoxybenzhydrol as an oil; a2) from 3-methoxy-4-benzyloxybenzaldehyde and l-bromo-4--fluorobenzene there is obtained 4-(benzyloxy)-4'-fluoro--3-methoxybenzhydrol as an oil; a3) from 3-methoxy-4-benzyloxybenzaldehyde and 1-bromo--2.6-difluorobenzene there is obtained 4-(benzyloxy)~ -2'.6'-difluoro-3-raethoxybenzhydrol as an oil; a4) from 3-raethoxy-4-benzyloxybenzaldehyde and l-bromo-2--chlorobenzene there is obtained 4-(benzyloxy)-21-chloro--3-methoxybenzhydrol as an oil; a5) from 3-raethoxy-4-benzyloxybenzaldehyde and l-bromo-3--chlorobenzene there is obtained 4-(benzyloxy)-3'-chloro--3-methoxybenzhydrol as an oil; a6) from 3-methoxy-4-benzyloxybenzaldehyde and l-bromo-4--chlorobenzene there is obtained 4-(benzyloxy)-41-chloro--3-methoxybenzhydrol as an oil; a7) from 4-benzyloxy-3-methoxybenzaldehyde and 2-bromotoluene there is obtained 4-(benzyloxy)-3-methoxy--21-methylbenzhydrol as an oil; a8) from 3-methoxy-4-benzyloxybenzaldehyde and 4-bromotoluene there is obtained 4-(benzyloxy)-3-methoxy- 2 -4'-methylbenzhydro1 as an oil; a9) from 3-raethoxy-4-benzyloxybenzaldehyde and 1-bromobenzonitrile there is obtained 4-(benzyloxy)-2 -cyano-3-methoxybenzhydrol as an oil and "alO) from 3-methoxy-4-benzyloxybenzaldehyde and l-brorao-2-trifluoroniethylbenzene there is obtained 4-(benzyloxy)-3-methoxy-2'-(trifluoromethyl)benzhydrol as an oil. b) 69.8 g of 4-(benzyloxy)-2'-fluoro-3-methoxybenzhydrol (dissolved in 600 ml of methylene chloride) are treated within 30 minutes at 20° with 45.3 g of pyridinium chlorochroraate and stirred at 20° for 3 hours. The precipitate formed is subsequently filtered off and washed with methylene chloride. The filtrate is evaporated and the residue is filtered on 100 g of silica gel with ether. After recrystallization from ether there is obtained 4-(benzyloxy)-2'-fluoro-3-methoxybenzophenone of m.p. 118-120°.
In an analogous manner: bl) From 4-(benzyloxy)-31-fluoro-3-methoxybenzhydrol there is obtained 4-(benzyloxy)-3'-fluoro-3-methoxybenzophenone as an amorphous solid; b2) from 4-(benzyloxy)-4'-fluoro-3-methoxybenzhydrol there is obtained 4-(benzyloxy)-4'-fluoro-3-methoxybenzophenone of m.p. 99-101° (from ether/hexane); b3) from 4-(benzyloxy)-2', 61-difluoro-3-methoxybenzhydrol there is obtained 4-(benzyloxy)-2',6'-difluoro-3-methoxy-benzophenone of m.p. 139-141° (from methylene chloride/ether); i ~ y-0 rs 2 AO «L - ioi - / i ~y J ,3 b4) from 4-(benzyloxy)-2'-chloro-3-methoxyben2hydrol there is obtained 4-(benzyloxy)-2'-chloro-3-methoxybenzophenone of m.p. 128-130° (from ether): bb) from 4-(benzyloxy)-3'-chloro-3-methoxybenzhydrol there is obtained 4-(benzyloxy)-31-chloro-3-methoxybenzophenone as an amorphous solid; b6) from 4-(benzyloxy)-4'-chloro-3-methoxyben2hydrol there is obtained 4-(benzyloxy)-4'-chloro-3-methoxybenzophenone of m.p. 106-108° (from methylene chloride/hexane); b7) from 4-(benzyloxy)-3-raethoxy-2•-methylbenzhydrol there 15 is obtained 4-(benzyloxy)-3-methoxy-2'-methylbenzophenone of m.p. 86-88° (from isopropyl ether): b8) from 4-(benzyloxy)-3-methoxy-41-methylbenzhydrol there is obtained 4-(benzyloxy)-3-methoxy-41-methylbenzophenone 20 of m.p. 79-81° (from ether/hexane); b9) from 4-(benzyloxy)-2'-cyano-3-methoxybenzhydrol there is obtained 4-(benzyloxy)-21-cyano-3-methoxybenzophenone as an amorphous solid and blO) from 4-(benzyloxy)-3-methoxy-2'-(trifluoro- methyl)benzhydrol there is obtained 4-(benzyloxy)-3--methoxy-21 -(trifluoromethyl)benzophenone of m.p. 103-105° (from ether). c) 170 ml of 33 percent hydrobromic acid in glacial acetic acid are added at 20-25° within 20 minutes to 42.4 g of 4-(benzyloxy)-21-fluoro-3-methoxybenzophenone (dissolved in 450 ml of methylene chloride). After 35 stirring at 20° for 1.5 hours the reaction mixture is poured into 750 ml of ice-water; the methylene chloride phase is separated and the aqueous phase is extracted a further twice with 200 ml of methylene chloride. The combined methylene chloride phases are washed with 1200 ml of water, dried over sodium sulphate and evaporated. In 5 order to remove the resulting benzyl bromide, the oily residue is treated with hexane and decanted off. There is obtained 2'-fluoro-4-hydroxy-3-methoxybenzophenone as a yellowish oil which can be used directly in the subsequent reaction step.
In an analogous manner: cl) From 4-(benzyloxy)-3'-fluoro-3-raethoxybenzophenone there is obtained 3'-fluoro-4-hydroxy-3-methoxybenzo-15 phenone of m.p. 133-135° (from methylene chloride/low--boiling petroleum ether); c2) from 4-(benzyloxy)-4'-fluoro-3-methoxybenzophenone there is obtained 4'-fluoro-4-hydroxy-3-methoxybenzo-20 phenone of m.p. 139-141° (from ether); c3) from 4-(benzyloxy)-2',6'-difluoro-3-methoxybenzo-phenone there is obtained 2'.6'-difluoro-4-hydroxy-3--methoxybenzophenone of m.p. 130-132° (from methylene 25 chloride/low-boiling petroleum ether); c4) from 4-(benzyloxy)-21-chloro-3-methoxybenzophenone there is obtained 2•-chloro-4-hydroxy-3-methoxybenzo-phenone as an amorphous solid; c5) from 4-(benzyloxy)-3'-chloro-3-methoxybenzophenone there is obtained 31-chloro-4-hydroxy-3-methoxybenzo-phenone of m.p. 136-138° (from methylene chloride); c6) from 4-(benzyloxy)-4'-chloro-3-methoxybenzophenone there is obtained 4'-chloro-4-hydroxy-3-methoxybenzo-phenone of m.p. 114-116° (from methylene chloride/low- 2 1949 - 103 - ~ -boiling petroleum ether): c7) from 4-(benzyloxy)-3-roethoxy-2'-methylbenzophenone there is obtained 4-hydroxy-3-methoxy-2'-methylbenzophenone of m.p. 103-105° (from isopropyl ether); c8) from 4-(benzyloxy)-3-methoxy-4'-methylbenzophenone there is obtained 4-hydroxy-3-methoxy-4'-methylbenzophenone of m.p. 103-105° (from ether/low-boiling petroleum ether); c9) from 4-(benzyloxy)-2'-cyano-3-methoxybenzophenone there is obtained 21-cyano-4-hydroxy-3-methoxybenzophenone of m.p. 124-126° (from ether/n-hexane) and clO) from 4-(benzyloxy)-3-methoxy-2'-(trifluoro methyl )benzophenone there is obtained 4-hydroxy-3-methoxy--2'-(trifluoromethyl)benzophenone of m.p. 115-117° (from ether). d) 7.8 ml of 65 percent nitric acid are added dropwise at 20° within 20 minutes to 29.4 g of 21-fluoro-4-hydroxy-3--methoxybenzophenone (dissolved in 450 ml of acetic acid). After stirring for 1.5 hours the reaction mixture is poured into 2 1 of ice-water and the precipitate formed is filtered off. washed with water and dissolved in methylene chloride. The methylene chloride solution is washed with water, dried over sodium sulphate and evaporated. The residue is recrystallized from methanol. There is obtained 21-fluoro-4-hydroxy-3-methoxy-5-ni trobenzophenone of m.p. 127-129° .
In an analogous manner: dl) From 31-fluoro-4-hydroxy-3-methoxybenzophenone there is obtained 3•-fluoro-4-hydroxy-3-methoxy-5-nitrobenzo-phenone of m.p. 168-170° (from methanol); 2 1 Q A Q | $ -V I — d2) from 4'-fluoro-4-hydroxy-3-methoxybenzophenone there is obtained 4•-fluoro-4-hydroxy-3-methoxy-5-nitrobenzo-phenone of m.p. 126-128° (from ether); d3) from 2'.6 •-difluoro-4-hydroxy-3-methoxybenzophenone there is obtained 2'.61-difluoro-4-hydroxy-3-methoxy-5--nitrobenzophenone of m.p. 147-149° (from methanol); d4) from 2'-chloro-4-hydroxy-3-methoxybenzophenone there is obtained 2'-chloro-4-hydroxy-3-methoxy-5-nitrobenzo-phenone of m.p. 123-125° (from ether); d5) from 3'-chloro-4-hydroxy-3-methoxybenzophenone there is obtained 3'-chloro-4-hydroxy-3-methoxy-5-nitrobenzo-phenone of m.p. 152-154° (from methanol); d6) from 4'-chloro-4-hydroxy-3-methoxybenzophenone there is obtained 4'-chloro-4-hydroxy-3-methoxy-5-nitrobenzo-phenone of m.p. 129-131° (from methylene chloride/low--boiling petroleum ether); d7) from 4-hydroxy-3-methoxy-2'-methylbenzophenone there is obtained 4-hydroxy-3-methoxy-2'-methyl-5-nitrobenzo-phenone of m.p. 125-127° (from ethanol); d8) from 4-hydroxy-3-methoxy-41-methylbenzophenone there is obtained 4-hydroxy-3-methoxy-4'-methyl-5-nitrobenzo-phenone of m.p. 137-139° (from methylene chloride/ether); d9) from 2'-cyano-4-hydroxy-3-methoxybenzophenone there i obtained 2'-cyano-4-hydroxy-3-methoxy-5-nitrobenzophenone of m.p. 163-164° (from methanol); dlO) from 4-hydcoxy-3-methoxy-2'-(trifluoromethyl)- benzophenone there is obtained 4-hydroxy-3-methoxy-5--nitro-2'-(trifluoromethyl)benzophenone of m.p. 138-140° 7 1Q A jL 0 * - (from methylene chloride/low-boiling petroleum ether); dll) from 4-hydroxy-3.4'-dimethoxybenzophenone there is obtained 4-hydroxy-3'.4'-dimethoxy-5-nitrobenzophenone of m.p. 134-136° (from methanol) and dl2) from 4-hydroxy-3.3'.41-triraethoxybenzophenone there is obtained 4-hydroxy-5-nitro-3.3•.4'-trimethoxy-benzophenone of m.p. 178-180° (from methanol). e) 24.8 g of 2'-fluoro-4-hydroxy-3-methoxy-5-nitrobenzo-phenone (dissolved in 120 ml of glacial acetic acid. 100 ml of 33 percent hydrobromic acid in glacial acetic acid and 68 ml of 48 percent aqueous hydrobromic acid) are boiled under reflux for 4 hours. The reaction mixture is subsequently evaporated under reduced pressure and the residue is distilled with toluene. The residue is dissolved in methylene chloride, washed with water, dried over sodium sulphate, filtered and evaporated. The product is crystallized from methylene chloride/low-boiling petroleum ether. There is obtained 2'-fluoro-3.4--dihydroxy-5-nitrobenzophenone of m.p. 169-171°.
In an analogous manner: el) From 3'-fluoro-4-hydroxy-3-methoxy-5-nitrobenzophenone there is obtained 3'-fluoro-3.4-dihydroxy-5-nitrobenzo-phenone of m.p. 124-126° (from methylene chloride); e2) from 4'-fluoro-4-hydroxy-3-methoxy-5-nitrobenzophenone there is obtained 41-£luoro-3.4-dihydroxy-5-nitrobenzo-phenone of m.p. 171-173° (from methylene chloride); e3) from 2',6'-difluoro-4-hydroxy-3-methoxy-5-nitrobenzo-phenone there is obtained 21.6•-difluoro-3.4-dihydroxy-5--nitrobenzophenone of m.p. 194-196° (from methanol); ^ ■ Q A - 106 - I J> •~V e4) from 2'-chloro-4-hydroxy-3-methoxy-5-nitrobenzo-phenone there is obtained 2'-chloro-3,4-dihydroxy-B-nitro-benzophenone of m.p. 129-131° (from methylene chloride/ low-boiling petroleum ether); e5) from 3'-chloro-4-hydroxy-3-methoxy-5-nitrobenzo-phenone there is obtained 3'-chloro-3.4-dihydroxy-5-nitro-benzophenone of m.p. 143-145° (from methylene chloride/ low-boiling petroleum ether); e6) from 4'-chloro-4-hydroxy-3-methoxybenzophenone there is obtained 4'-chloro-3,4-dihydroxybenzophenone of m.p. 174-176° (from methylene chloride); e7) from 4-hydroxy-3-methoxy-2'-methyl-5-nitrobenzo-phenone there is obtained 3,4-dihydroxy-2'-methyl-5-nitro-benzophenone of m.p. 164-166° (from methylene chloride); e8) from 4-hydroxy-3-methoxy-4'-methyl-5-nitrobenzo-phenone there is obtained 3,4-dihydroxy-4'-methyl-5-nitro-benzophenone of m.p. 146-148° (from methylene chloride); e9) from 21-cyano-4-hydroxy-3-methoxy-5-nitrobenzophenone there is obtained 21-cyano-3.4-dihydroxy-5-nitrobenzo-phenone of m.p. 159-161° (from methanol); elO) from 4-hydroxy-3-methoxy-5-nitro-2'-(trifluoromethyl ) benzophenone there is obtained 3,4-dihydroxy-b--nitro-2'-(trifluoromethyl)benzophenone of m.p. 146-148° (from methanol); ell) from 4-hydroxy-3,4'-dimethoxy-5-nitrobenzophenone there is obtained 5-nitro-3.4,4'-trihydroxybenzophenone of m.p. 212-214° (from methanol/methylene chloride) and el2) from 4-hydroxy-5-nitro-3.31,41-trimethoxybenzo- phenone there is obtained 5-nitro-3,3',4,4'-tetrahydroxy-benzophenone of m.p. 222-224° (from ether).
Example 77 A suspension of 13.8 g of 2-bromo-34•-dihydroxy-5'--nitroacetophenone is treated with 9.0 g of l-(phenethyl)--2-thiourea in 150 ml of n-butanol and the mixture is heated to boiling under reflux for 3 hours. After cooling to room temperature the crystals are filtered off under suction and crystallized from n-butanol. There is obtained 3-nitro-5-[2-(phenethylamino)-4-thiazolyl]pyrocatecho1 hydrobromide of m.p. 249-251°.
Example 78 In analogy to Example 38. from 2-bromo-3',4'--dihydroxy-5'-nitroacetophenone and 2-aminobenzophenone there is obtained 2-(3.4-dihydroxy-5-nitrobenzoyl)-3--phenylindole of m.p. 196-198° (from isopropanol).
Example 79 A suspension of 8.3 g of 2-bromo-34'-dihydroxy-5•--nitroacetophenone is treated with l-(l-adamantyl)-2--thiourea in 90 ml of n-butanol and the mixture is heated to boiling under reflux for 4 hours. After cooling to room temperature the crystals are filtered off under suction and recrystallized from n-butanol. There is obtained 5-[2-(1-adamantylamino)-5-thiazolyl]-3-nitropyrocatechol hydrobromide of m.p. 245-247°.
Example 80 A suspension of 2.6 g of (3.4-dihydroxy-5-nitro-benzoyl)methyl acetate in 20 ml of ethanol and 20 ml of IN hydrochloric acid is heated to boiling under reflux for 5 hours. The reaction mixture is then evaporated, the residue is distilled with toluene and then recrystallized 5 from ethanol. There is obtained 2.3'.41-trihydroxy-5'--nitroacetophenone of m.p. 208-210°.
Example 81 A solution of 4.0 g of n-hexyl 3,4-dihydroxy-5-nitro- phenylglyoxylate and 1.5 g of diaminomaleonitrile in 35 ml of ethanol is heated to boiling under reflux for 24 hours. The alcohol is then distilled off, the residue is dissolved in ether, washed with water, dried over sodium sulphate, filtered and evaporated. There is obtained 6-hydroxy-5-(3,4-dihydroxy-5-nitrophenyl)-2,3-pyrazine-dicarbonitrile of m.p. >300° (from ether/methylene chloride).
Example 82 a) 4.2 g of 5-(bromoacetyl)-2,3-dimethoxybenzonitrile dissolved in 150 ml of methylene chloride are treated with 8.9 ml of boron tribromide. The reaction mixture is stirred at 20° for 18 hours. It is subsequently poured into 220 ml of saturated sodium hydrogen carbonate solution and 100 g of ice. adjusted to pH 6 with glacial acetic acid and extracted with ethyl acetate. The organic phase is washed with water, dried over sodium sulphate and evaporated. There is obtained 5-(bromoacetyl)-2,3--dihydroxybenzonitrile as an amorphous solid. b) 3.8 g of 5-(bromoacetyl)-2,3-dihydroxybenzonitrile dissolved in 25 ml of N.N-dimethylformamide are treated with N-phenylthiourea and stirred at 100° for 5 hours. Thereafter, the solvent is removed by evaporation. The residue is treated with 200 ml of IN sodium carbonate _ — solution and extracted three times with 100 ml of methylene chloride each time. The combined organic phases are washed with water, dried over sodium sulphate and evaporated. The residue is chromatographed on 30 g of silica gel with ethyl acetate. The thus-obtained crude product is treated with 40 ml of IN hydrochloric acid, evaporated and crystallized from acetone. There is obtained 5-(2-anilino-4-thiazolyl)-2,3-dihydroxybenzo-nitrile hydrochloride of m.p. 245-247°.
Example 83 a) 25 ml of tert.-butyllithium solution (1.4M in hexane) are added dropwise at -70° within 10 minutes to 10 g of 4-(benzyloxy)-3-methoxy-bromobenzene dissolved in 100 ml of tetrahydrofuran. After stirring at -70° for 1 hour 5 g of quinoline-4-carbaldehyde dissolved in 50 ml of tetrahydrofuran are added dropwise within 30 minutes. The reaction mixture is stirred at -40° for 1 hour and at -5° for 1 hour, poured into 200 ml of water and adjusted to pH 4 with glacial acetic acid. The mixture is extracted three times with 50 ml of ether each time. The combined ether phases are washed with water, dried over sodium sulphate and evaporated. There is obtained a-[4-(benzyloxy) -3-methoxyphenyl ]-4-quinolinemethanol as an amorphous solid. b) 9.4 g of a-[4-(benzyloxy)-3-methoxyphenyl]-4--quinolinemethanol dissolved in 200 ml of methylene chloride are treated with 6.5 g of pyridinium chloro-chromate. whereupon the mixture is stirred at room temperature for 3 hours. The insoluble constituents are subsequently filtered off. The filtrate is evaporated and the residue is chromatographed on 150 g of silica gel with ethyl acetate. There is thus obtained 4-(benzyloxy)-3--methoxyphenyl 4-quinolyl ketone as an amorphous solid. ) -. n 2 A c) 15 ml of 33 percent hydrobromic acid in glacial acetic acid are added dropwise within 5 minutes at room temperature to 7.5 g of 4-(benzyloxy)-3-methoxyphenyl 5 4-quinolyl ketone dissolved in 150 ml of methylene chloride. After stirring at 20° for 4.5 hours the reaction mixture is poured portionwise into 250 ml of saturated sodium bicarbonate solution. The methylene chloride phase is separated; the aqueous phase is extracted a further 10 twice with 100 ml of methylene chloride each time. The combined methylene chloride phases are dried over sodium sulphate and evaporated. The residue is recrystallized from methylene chloride/hexane. There is obtained 4-hydroxy-3-methoxyphenyl 4-quinolyl ketone of m.p. 190-192°. d) 0.37 ml of 65 percent nitric acid is added dropwise at room temperature to 1.3 g of 4-hydroxy-3-methoxyphenyl 4-quinolyl ketone. After stirring for 3 hours the reaction mixture is poured into ice-water, adjusted to pH 6 with conc. ammonia and the precipitate formed is filtered off. The thus-obtained residue is heated under reflux in 20 ml of acetonitrile, whereupon the crystals are filtered off at 0°. There is obtained 4-hydroxy-3-methoxy-5-nitrophenyl 4-quinolyl ketone of m.p. 246-248°. e) 1 g of 4-hydroxy-3-methoxy-5-nitrophenyl 4-quinolyl ketone dissolved in 30 ml of 48 percent aqueous hydrobromic acid is stirred at 100° for 18 hours. After cooling to room temperature the reaction mixture is diluted with 30 ml of water and the precipitate is filtered off under suction. There is obtained 3.4-dihydroxy-5-nitrophenyl 4-quinolyl ketone hydrobromide of m.p. 273-275° (from acetonitrile).
- Ill - Example 84 a) 18.8 ml of n-butyllithium solution (1.6M in hexane) are added dropwise at -50° within 10 minutes to 4.03 g of thiophene dissolved in 40 ml of tetrahydrofuran. After stirring at -50° for 30 minutes 6.3 g of 3,4-dimethoxy-5--nitrobenzaldehyde dissolved in 100 ml of tetrahydrofuran are added dropwise within 30 minutes. The reaction mixture is stirred at -50° for 1 hour at 0° for 30 minutes and poured into 100 ml of 2N sulphuric acid. The mixture is extracted three times with 100 ml of ether each time; the combined ether phases are washed with sodium chloride solution, dried over sodium sulphate, filtered and evaporated. There is obtained a-(3,4-dimethoxy-5--nitrophenyl)-2-thiophenemethanol of m.p. 79-81° (from methylene chloride/hexane). b) 9.9 g of a-(3,4-dimethoxy-5-nitrophenyl)-2--thiophenemethanol dissolved in 300 ml of acetone are treated with 90 g of manganese dioxide and heated under reflux for 4 hours. The manganese dioxide is removed by suction filtration and the filtrate is evaporated. There is obtained 3.4-dimethoxy-5-nitrophenyl 2-thienyl ketone of m.p. 102-104° (from methylene chloride/hexane). c) 2 g of 3,4-dimethoxy-5-nitrophenyl 2-thienyl ketone are stirred at 100° for 8 hours in a mixture of 20 ml of 30-33 percent hydrobromic acid in glacial acetic acid and 20 ml of 48 percent aqueous hydrobromic acid. The reaction mixture is subsequently evaporated to dryness. The residue is taken up in ethyl acetate, washed with water, dried over sodium sulphate and filtered, and the filtrate is evaporated. After recrystallization from ethyl acetate/ hexane there is obtained 3,4-dihydroxy-5-nitrophenyl 2-thienyl ketone of m.p. 155-157°. ■j. j C,A Example A 2 1949$ The interlocking gelatine capsules of the following ^ composition can be manufactured in a manner known per se: Ingredients Amount in ma/capsules L-Dopa 100 ^ lo Benserazide hydrochloride 29.3 3.4-Dihydroxy-5-nitrophenyl 4-pyridyl ketone 25 Gelatine 1 Mg stearate 1 Avicel 93.7 Mannitol 100 Capsule fill weight 350 mg 219496

Claims (3)

  1. WHAT WE CLAIM IS: 1. Compounds of the general formula H(VN- 1 'J - Rc1 HO/ N-XBb 10 wherein Ra signifies nitro or cyano. Rb signifies hydrogen or halogen, Rc' signifies nitro, cyano or the group -(A)n-(Q)m-R1:L or - (A) n-Q-R21, A signifies vinylene optionally substituted by lower alkyl, n signifies the number O or 1, m signifies the 11 ... 31 15 number O or 1, R signifies the group -COR , a carbocyclic aromatic group,or an aromatic or partially unsaturated heterocyclic group attached via 21 a carbon atom, R signifies an optionally substituted. saturated or partially unsaturated, lower 31 20 hydrocarbon residue, R signifies hydroxy, amino, an optionally substituted, saturated or partially unsaturated, lower hydrocarbon residue attached via an oxygen atom or an imino or lower alkylimino group or a saturated, N-containing heterocyclic group attached 25 via a ring nitrogen atom, Q signifies the group 4 -CO- or >C=N-(Z)p-R , 2 signifies an oxygen atom or an imino group, p signifies the number O or 1 and 4 R signifies hydrogen or a saturated or partially unsaturated, lower hydrocarbon residue which is 30 optionally substituted and which is optionally attached via a carbonyl group, whereby Ra signifies 31 cyano when Rc1 signifies cyano or nitro and R has a significance different from hydroxy when m signifies the number O, 35 and ester and ether derivatives which are hydrolyzable under physiological conditions and pharmaceutically ■Q" v>\\ \ - Si \ *2 ¥' ^ i: f. V"v ~ ?\ 9496 - 114 - acceptable salts thereof.
  2. 2. Compounds according to claim 1. wherein Rb is situated in the p-position to Ra.
  3. 3. Compounds according to claim 1 or 2. wherein Ra signifies nitro. (~y 4. Compounds according to any one of claims 1-3, 10 wherein Rb signifies hydrogen, chlorine or fluorine. 5. Compounds according to claim 4, wherein Rb signifies hydrogen. 15 6- Compounds according to any one of claims 1-5, wherein Rc' signifies the group -CO-R11 and R11 signifies an aromatic mononuclear carbocyclic group,or an aromatic mononuclear heterocyclic group,with 1-3 nitrogen atoms as the hetero ring member(s)jwhich is attached via a 20 carbon atom. 7. Compounds according to claim 6, wherein R11 signifies a phenyl group optionally mono- or disubstituted by halogen, trifluoromethyl, cyano. hydroxy or lower alkyl 25 or a pyridyl group. 30 8. 3,4-Dihydroxy-5-nitrobenzophenone. 9. 21-Fluoro-3,4-dihydroxy-5-nitrobenzophenone. 10. 3,4-Dihydroxy-5-nitrophenyl 4-pyridyl ketone. 11. A process for the manufacture of compounds according to any one of claims 1-10, of ester and ether 35 derivatives which are hydrolyzable under physiological conditions and of pharmaceutically acceptable salts •o.\ ■*Y A <P\ cvi r,i.' 2J949G - 115 - thereof, which process comprises a) cleaving off the lower alkyl ether group(s) in a compound of the general formula RO. 10 15 or 20 25 30 • • 1 " r, . *"Rc R'° II wherein one of the symbols R and R' signifies lower alkyl and the other signifies hydrogen or lower alkyl and Ra. Rb and Rc' have the significance given in claim l. b) reacting a compound of the general formula HO. HO' A\!J'(A)n-C0-CH2-x Rb Ibl wherein X signifies a leaving group and Ra. Rb, A and n have the significance given in claim 1, with a thioamide, thiourea, thiocarboxylic acid hydrazide, thiosemicarbazide, amidine. guanidine. amidrazone, aminoguanidine. cyclic amidine. 1,2-diamine. 1.2-amino-thiol or a 1,2-aminoalcohol and. if desired, dehydrogen-ating the cyclocondensation product obtained, for the manufacture of a compound of the general formula 35 n - 116 - Ib7 V'-CAJn-Q" HO H<\ /XX'-'^n-Q1 Rb wherein Ra, Rb. A and n have the significance given in claim 1 and signifies a group of the formula yRe ,NHRf K, kt N=y ^N-N N-N ^ ' _/ -V ) —Re -•( ).-NHRf O ^.-1 (a> ' (b)/ *-S (c) , • -S N=0 yN-'jT yN-S " (1)' (k) °r _N/*~Rh (k'}' XRf M ^ /Rg \ c/*~Rh or >-Rh (m) , (d) , /Re yNHRf /N=i /N=i /N=N N=N \_L^' "X-AH <f>' "s.v'"Re (9)-"*w'"NHRf (h) N=«^~\ N /R<* /R^ -0 CS in which Re signifies hydrogen. C -alkyl, C ** 3—7 3 — 7 -cycloalkyl, aryl, heteroaryl, aryl-lower alkyl or heteroaryl-lower alkyl, Rf signifies hydrogen, aryl. aryl-lower alkyl. lower alkyl, lower alkoxycarbonyl. heteroaryl. heteroaryl-lower alkyl. Cg -bicyclo-/**\ alkyl or C_^ ^-cycloalkyl, Rg and Rh each signify hydrogen, cyano, lower alkyl. C3 7~cycloalkyl. aryl, aryl-lower alkyl, heteroaryl or heteroaryl-lower alkyl or Rg and Rh together with the two carbon atoms to which they are attached signify a carbocyclic aromatic group or an aromatic or partially unsaturated, heterocyclic group, the dotted line 4 signifies an optional bond and Q together with .th carbon atom and the nitrogen atom signify aSrc-agomatic or partially unsaturated, heterocyclic • group whi5^ contains at least one nitrogen atom as a hetero ring member, or - 117 - c) reacting a compound of the general formula • , "-(A) -CO-COOR* lb2 / ^ x n HO ' Rb wherein R" signifies lower alkyl and Ra. Rb, A and n have the significance given in claim 1, with a 1,2-diamine, 1,2-aminothiol, 1,2-aminoalcohol. semicarbazide, thiosemicarbazide, amidrazone or an araino-guanidine and, if desired, dehydrogenating the cyclocondensation product obtained, for the manufacture of a compound of the general formula 21 ?M;SG \ ' "-<A)n-Q2 H0N H0/ v r.2NOV 1990' in which Ra, Rb, A and n have the significance given in claim 1 and Q2 signifies a group of the formula : Rg /Rg /Rg . .N-* /N-« -< y~Rh (n), -< *)*-Rh (o) ' "•( 'V-Rh (P), x*-s s—o </ XRf 0/ (/ ✓N-Nv y?N-N^ >-0H (q) , -•( / —SH (r), — • -Re (s) or 0>-<H /N-N. -•( )--NHRf (t) , cr h in which Re, Rf, Rg and Rh and the dotted line have the above significance, or :&m.... , _ -v,-,. „v_ .... i, «...,.*.■ '.'.ii. . -f . t.jv :«•' "^i4.r ■.» - -v. 2.19 4 9 G - 118 d) reacting a compound of formula lb1 above with A~2N()V1990 fl-aminocarbonyl compound, for the manufacture of a "?<«v ,<•** ' I x compound of the general formula "~ HO. 'W \ II -o3 ^xv;(A)"~Q Rb HO' lb* wherein Ra, Rb, A and n have the significance given in claim 1 and Q3 signifies the group of the formula \ / N • • II II /Rg (u) Re / \ Rh i in which Re, Rf, Rg, Rh and the dotted line have the above significance, or e) converting the carboxaldehyde group(s) in a compound of the general formula C^HO H°s ' » HO N*\b -Rc' 9N H0\.^ HO III. I v •-CHO Ia2 or / ^ N H0 * Rb HO <fHO \ ^*\ I I •-CHO Rb IV wherein Rc"1 signifies nitro. cyano or the group -(A)n~R12 and R12 signifies the group -COR a carbocyclic aromatic group^or an aromatic or partially unsaturated heterocyclic group attached via 31 a carbon atom and Ra. Rb, A, n and R have the significance given in claim 1, or in a di-O-lower alkanoyl derivative thereof into the cyano group(s), for the manufacture of a compound of the general formula lb wherein Ra signifies cyano,Rc' signifies nitro, cyano or the group -(A)n—R-®-^ and R^-2 signifies the group -COR3^, a carbocyclic aromatic group or an I .WW,-,;-. - 119 - -2 NOV 1990 aromatic or partially unsaturated, heterocyclic group^ggj^J attached via a carbon atom and Rb, A, n and R3^ have the significance given in claim 1, or f) reacting a di-O-lower alkanoyl derivative of a carboxylic acid of the general formula Pa Ra hon >-N hoN A • • • « 'i — f _-C00H la3 or i 'J A v"-(A)n-C00H la or Av"-(A)n-C0-C00H Ib3 i N.*_. HO " H0 * Rb H0 * Rb wherein Ra. Rb, A and n have the significance given in claim 1, in the presence of a condensation agent or a reactive derivative or a di-O-lower alkanoyl derivative of a 3 3 carboxylic acid of formula la or Ib with a compound of the general formula 5 6 7 HO-R V or HNR R VI 5 wherein R signifies an optionally substituted. saturated or partially unsaturated, lower hydrocarbon 6 residue. R signifies hydrogen or lower alkyl and 7 . . R signifies hydrogen or an optionally substituted, saturated or partially unsaturated, lower hydrocarbon 6 7 . residue or R and R together with the nitrogen atom signify a saturated N-containing heterocyclic group. for the manufacture of a compound of the general formula Ib in which Rc' signifies the group 3 2 3 2 -(A)n-(CO)m-COR and R signifies amino, an optionally substituted, saturated or partially unsaturated, lower hydrocarbon residue attached via an oxygen atom or an imino or lower alkylimino group or a saturated, N-containing heterocyclic group attached via a ring nitrogen atom and A, n and m have the significance given in claim 1, or i'-i'&Mvi??*^* V ' o c e -21 34% 1 % - 120 - 2 g) hydrolyzing a compound of formula Ib or a compound of the general formula 8 ?a R °S ^'S • • 8 Av1J-rC Ib4 R O N«* L Rb 8 wherein R signifies lower alkanoyl and Ra, Rb and Rc' have the significance given in claim 1. for the manufacture of a compound of the general formula Ib, or h) reacting a compound of the general formula HCV'x ! X'J-C°R'" Ib5 HO x-\b wherein Ra and Rb have the significance given in claim 1 and R"' signifies hydrogen or lower alkyl, or a di-O-lower alkanoyl derivative thereof in the w r presence of a secondary amine with a compound of the foS general formula f/^ 23 ch3co-r VII \ 23 —■*" wherein R signifies an optionally substituted, saturated or partially unsaturated, lower hydrocarbon Vv \ residue. for the manufacture of a compound of the general formula • • lb10 I II 01 iD A v'-C (R"!) =CH-C0—R H0 * Rb 21949(3 121 wherein Ra and Rb have the significance given in claim.1 and R"1 and R23 have the above significan and the corresponding di-O-lower alkanoyl derivatives thereof, or KD wherein Ra, Rb, A and n have the significance given in claim 1 and R" has the above significance, with a hydrazine or an amidine, for the manufacture of a compound of the general formula i) reacting a compound of the general formula Ib6 lb" wherein Ra, Rb, A and n have the significance given in claim 1 and Q5 signifies the group / \ .Rf (v) or (w) OH in which Re and Rf, have the above significance, or - 122 - 21043 b' j) reacting a compound of formula Ib above in which m signifies the number 1 and Q signifies the group -CO- with a compound of the general formula H2N-(Z)p-R4 VII 4 • A . O// wherein Z. p and R have the significance give in claim 1. for the manufacture of a compound of the general formula H°X^. .Ri I 11 11\ \ r' /'A"( )n\ m -R4 lbl2 HO • \b N-(Z)p K wherein Ri signifies the" group -COR31, a carbocyclic. aromatic group or an aromatic or partially unsaturated, heterocyclic group attached via a carbon atom or an optionally substituted, saturated or partially unsaturated, lower hydrocarbon residue 31 4 and Ra, Rb, R , R . A. Z, n and p have the significance given in claim 1 , and, if desired, k) converting a compound of formula Ib above into an ester or ether derivative which is hydrolyzable under physiological conditions or into a pharmaceutically acceptable salt thereof. 12. A pharmaceutical preparation containing a compound of the general formula H0\ • • 1 " r, - T5 v-Rc Ia HO N • %,. Rb - 123 - 219/J3G O wherein Ra signifies nitro or cyano. Rb signifies hydrogen or halogen, Rc signifies halogen, nitro. cyano or the group -(A)n-(Q)m-R or -(A)n~Q-R2. A signifies vinylene optionally Q substituted by lower alkyl. n signifies the number O or 1. m signifies the number O or 1. R signifies 3 the group -COR . a carbocyclic aromatic grouper an aromatic or partially unsaturated heterocyclic group 2 attached via a carbon atom. R signifies hydrogen or an optionally substituted, saturated or partially 3 unsaturated, lower hydrocarbon residue, R signifies hydroxy, amino, an optionally substituted, saturated or partially unsaturated, lower hydrocarbon residue attached via an oxygen atom or an imino or lower alkylimino group or a saturated, N-containing heterocyclic group attached via a ring nitrogen atom, Q . . 4 signifies the group -CO- or >C=N-(Z)p-R , Z signifies an oxygen atom or an imino group, p 4 O signifies the number O or 1 and R signifies hydrogen or a saturated or partially unsaturated. O 2.194.9 - 124 - lower hydrocarbon residue which is optionally substituted and which is optionally attached via a carbonyl group, or an ester or ether derivative which is hydrolyzable 5 under physiological conditions or a pharmaceutically acceptable salt thereof and a therapeutically inert carrier material. 13. A pharmaceutical preparation for inhibiting catechol-O-methyl-10 transferase, containing a compound of formula la defined in claim 12 or a compound according to any one of claims 1-10 and a therapeutically inert carrier material. 14. A pharmaceutical preparation for the treatment of Parkinson's 15 disease and of parkinsonism, containing L-dopa. a peripheral decarboxylase inhibitor, a compound of formula la defined in claim 12 or a compound according to any one of claims 1-10 and a therapeutically inert carrier material. 20 15. A medicament pack for the treatment of Parkinson's disease and of parkinsonism, consisting of a pharmaceutical preparation according to claim 13 and a pharmaceutical preparation containing L-dopa, a peripheral decarboxylase inhibitor 25 and a therapeutically inert carrier material. 30 35 i- i - 125 - 16. The use of a compound of formula la defined in claim 12 or a compound according to any one of claims 1-10 for the manufacture of medicaments which inhibit catechol--O-methyltransferase. 17. The use of a compound of formula la defined in claim 12 or a compound according to any one of claims 1-10 for the manufacture of medicaments^ or the treatment of Parkinson's disease and of parkinsonisn^in combination with L-dopa and a peripheral decarboxylase inhibitor. 18. A process for the manufacture of compounds according to any one of claims 1-10, of ester and ether derivatives which are hydrolysable under physiological conditions and of pharmaceutically acceptable salts thereof, substantially as hereinbefore described with reference to any one of Examples 1 to 84. 19. Compounds according to any one of claims 1-10, whenever prepared according to the process claimed in claim 11 or claim 18. 20. A pharmaceutical preparation containing a compound of formula la defined in claim 12, substantially as hereinbefore described with reference to Example A. dated this z day of **
NZ219496A 1986-03-11 1987-03-04 Pyrocatechole derivatives and their pharmaceutical compositions NZ219496A (en)

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