NO304655B1 - Fremgangsmate for pavisning av polymorfisme pa basis av nukleotidforskjeller - Google Patents
Fremgangsmate for pavisning av polymorfisme pa basis av nukleotidforskjeller Download PDFInfo
- Publication number
- NO304655B1 NO304655B1 NO922800A NO923544A NO304655B1 NO 304655 B1 NO304655 B1 NO 304655B1 NO 922800 A NO922800 A NO 922800A NO 923544 A NO923544 A NO 923544A NO 304655 B1 NO304655 B1 NO 304655B1
- Authority
- NO
- Norway
- Prior art keywords
- primer
- dna
- nucleic acid
- extension
- extension product
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 61
- 239000002773 nucleotide Substances 0.000 title claims description 47
- 125000003729 nucleotide group Chemical group 0.000 title claims description 46
- 150000007523 nucleic acids Chemical class 0.000 claims description 49
- 108020004707 nucleic acids Proteins 0.000 claims description 47
- 102000039446 nucleic acids Human genes 0.000 claims description 47
- 230000003321 amplification Effects 0.000 claims description 35
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 35
- 238000006243 chemical reaction Methods 0.000 claims description 34
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- 239000000203 mixture Substances 0.000 claims description 14
- 108091034117 Oligonucleotide Proteins 0.000 claims description 13
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 11
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 11
- 230000000295 complement effect Effects 0.000 claims description 10
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- -1 nucleoside triphosphate Chemical class 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 2
- 239000005549 deoxyribonucleoside Substances 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
- 239000013615 primer Substances 0.000 description 100
- 108020004414 DNA Proteins 0.000 description 81
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
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- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
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- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 1
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- 241000589500 Thermus aquaticus Species 0.000 description 1
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- HGEVZDLYZYVYHD-UHFFFAOYSA-N acetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound CC(O)=O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O HGEVZDLYZYVYHD-UHFFFAOYSA-N 0.000 description 1
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- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 238000003391 densitometric scan Methods 0.000 description 1
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- 239000011159 matrix material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
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- 230000001568 sexual effect Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
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- 238000005382 thermal cycling Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Inverter Devices (AREA)
- Control Of Motors That Do Not Use Commutators (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/494,258 US5126239A (en) | 1990-03-14 | 1990-03-14 | Process for detecting polymorphisms on the basis of nucleotide differences |
PCT/US1991/000841 WO1991014001A2 (en) | 1990-03-14 | 1991-02-12 | A process for distinguishing nucleic acids on the basis of nucleotide differences |
Publications (3)
Publication Number | Publication Date |
---|---|
NO923544L NO923544L (no) | 1992-09-11 |
NO923544D0 NO923544D0 (no) | 1992-09-11 |
NO304655B1 true NO304655B1 (no) | 1999-01-25 |
Family
ID=23963743
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO922800A NO304655B1 (no) | 1990-03-14 | 1992-09-11 | Fremgangsmate for pavisning av polymorfisme pa basis av nukleotidforskjeller |
Country Status (17)
Country | Link |
---|---|
US (1) | US5126239A (fi) |
EP (1) | EP0520039B1 (fi) |
JP (1) | JP2818486B2 (fi) |
AT (1) | ATE115634T1 (fi) |
AU (1) | AU639565B2 (fi) |
BR (1) | BR9106151A (fi) |
CA (1) | CA2078132C (fi) |
DE (1) | DE69105959T2 (fi) |
DK (1) | DK0520039T3 (fi) |
ES (1) | ES2065684T3 (fi) |
FI (1) | FI924049A0 (fi) |
IL (1) | IL97503A (fi) |
NO (1) | NO304655B1 (fi) |
NZ (1) | NZ237396A (fi) |
PL (1) | PL166895B1 (fi) |
WO (1) | WO1991014001A2 (fi) |
ZA (1) | ZA911891B (fi) |
Families Citing this family (101)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5595870A (en) * | 1989-02-17 | 1997-01-21 | The Trustees Of Columbia University In The City Of New York | Identifying nucleic acids by restriction digestion and hybridization with random or pseudorandom oligonucleotides |
US5547839A (en) | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
WO1992003567A1 (en) * | 1990-08-24 | 1992-03-05 | The University Of Tennessee Research Corporation | Dna amplification fingerprinting |
US6074818A (en) * | 1990-08-24 | 2000-06-13 | The University Of Tennessee Research Corporation | Fingerprinting of nucleic acids, products and methods |
WO1992004458A1 (en) * | 1990-09-11 | 1992-03-19 | The Brookdale Hospital Medical Center | Drug resistance screening method |
WO1992007095A1 (en) * | 1990-10-15 | 1992-04-30 | Stratagene | Arbitrarily primed polymerase chain reaction method for fingerprinting genomes |
US5635354A (en) * | 1991-01-09 | 1997-06-03 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Method for describing the repertoires of antibodies (Ab) and of T-cell receptors (TcR) of an individual's immune system |
US5401630A (en) * | 1991-05-03 | 1995-03-28 | The United States Of America As Represented By The Secretary Of Agriculture | Species-specific DNA-DNA hybridization probe prepared using chromosome size DNA |
CA2077135A1 (en) * | 1991-08-30 | 1993-03-01 | Joh-E Ikeda | A method of dna amplification |
DK0534858T4 (da) * | 1991-09-24 | 2005-08-08 | Keygene Nv | Selektiv restriktionsfragmentamplifikation: en general fremgangsmåde til DNA-fingeraftryk-dannelse |
US7026115B1 (en) | 1991-09-24 | 2006-04-11 | Keygene N.V. | Selective restriction fragment amplification: fingerprinting |
US20100267023A1 (en) | 1992-09-24 | 2010-10-21 | Keygene N.V. | Selective restriction fragment amplification: fingerprinting |
EP0642590A1 (en) * | 1992-05-27 | 1995-03-15 | AMERSHAM INTERNATIONAL plc | Rna fingerprint analysis |
US5340728A (en) * | 1992-12-09 | 1994-08-23 | E. I. Du Pont De Nemours And Company | Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction |
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ZA911891B (en) | 1992-11-25 |
WO1991014001A3 (en) | 1991-10-31 |
ES2065684T3 (es) | 1995-02-16 |
IL97503A0 (en) | 1992-06-21 |
JPH05505528A (ja) | 1993-08-19 |
BR9106151A (pt) | 1993-03-09 |
FI924049L (fi) | 1992-09-10 |
DK0520039T3 (da) | 1995-02-20 |
ATE115634T1 (de) | 1994-12-15 |
NO923544L (no) | 1992-09-11 |
WO1991014001A2 (en) | 1991-09-19 |
US5126239A (en) | 1992-06-30 |
NZ237396A (en) | 1993-08-26 |
CA2078132A1 (en) | 1991-09-15 |
EP0520039A1 (en) | 1992-12-30 |
FI924049A0 (fi) | 1992-09-10 |
DE69105959D1 (de) | 1995-01-26 |
NO923544D0 (no) | 1992-09-11 |
PL166895B1 (pl) | 1995-06-30 |
IL97503A (en) | 1995-05-26 |
AU7782091A (en) | 1991-10-10 |
PL293188A1 (en) | 1992-11-02 |
JP2818486B2 (ja) | 1998-10-30 |
DE69105959T2 (de) | 1995-05-18 |
CA2078132C (en) | 1999-08-31 |
AU639565B2 (en) | 1993-07-29 |
EP0520039B1 (en) | 1994-12-14 |
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