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NO156916B - Stroemningskammer for vaeskestroemsfotometer. - Google Patents

Stroemningskammer for vaeskestroemsfotometer.

Info

Publication number
NO156916B
NO156916B NO852761A NO852761A NO156916B NO 156916 B NO156916 B NO 156916B NO 852761 A NO852761 A NO 852761A NO 852761 A NO852761 A NO 852761A NO 156916 B NO156916 B NO 156916B
Authority
NO
Norway
Prior art keywords
cells
particles
sec
pct
electrode
Prior art date
Application number
NO852761A
Other languages
English (en)
Other versions
NO156916C (no
NO852761L (no
Inventor
Harald B Steen
Original Assignee
Harald B Steen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harald B Steen filed Critical Harald B Steen
Priority to NO852761A priority Critical patent/NO156916C/no
Priority to US07/036,675 priority patent/US4737025A/en
Priority to PCT/NO1986/000050 priority patent/WO1987000282A1/en
Priority to JP61503776A priority patent/JPS62503192A/ja
Priority to AT86904422T priority patent/ATE43721T1/de
Priority to EP86904422A priority patent/EP0229814B1/en
Priority to DE8686904422T priority patent/DE3663744D1/de
Publication of NO852761L publication Critical patent/NO852761L/no
Publication of NO156916B publication Critical patent/NO156916B/no
Publication of NO156916C publication Critical patent/NO156916C/no

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N2015/1413—Hydrodynamic focussing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes

Landscapes

  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Chairs Characterized By Structure (AREA)
  • Special Spraying Apparatus (AREA)

Abstract

Den herværende oppfinnelse er en anordning som muliggjør at biologiske celler eller andre mikroskopiske partikler, båret av en mikroskopisk, laminær væskestrøm, føres enkeltvis over den åpne overflaten av et plant glass med en hastighet som kan varieres fra over 30 m/sek. til under 1 cm/sek, slik at cellenes/partiklenes fluorescens og lysspredning kan måles gjennom optikk plassert på begge sider av det plane glass. Anordningen omfatter en dyse (1) med hydrodynamisk fokusering som har sin lengdeakse i spiss vinkel med nevnte glass (2) og sin dyseåpning (4) i umiddelbar nærhet av glassets overflate. Videre vil cellenes/partiklenes elektriske isolerende volum samtidig kunne bestemmes ved at det i kontaktflaten mellom dysen (1) og nevnte glass (2) anbringes en elektrode (6) av inert metall. En konstant elektrolytisk strøm opprettholdes mellom denne elektrode (6) og en elektrode (7) i dysens væsketilførsel. Cellenes/partiklenes elektrisk isolerende volum kan måles ved de spenningspulser mellom nevnte elektroder de forårsaker idet de passerer gjennom dyseåpningen.
NO852761A 1985-07-10 1985-07-10 Stroemningskammer for vaeskestroemsfotometer. NO156916C (no)

Priority Applications (7)

Application Number Priority Date Filing Date Title
NO852761A NO156916C (no) 1985-07-10 1985-07-10 Stroemningskammer for vaeskestroemsfotometer.
US07/036,675 US4737025A (en) 1985-07-10 1986-07-10 Flow chamber device for flow cytometers
PCT/NO1986/000050 WO1987000282A1 (en) 1985-07-10 1986-07-10 A flow chamber device for flow cytometers
JP61503776A JPS62503192A (ja) 1985-07-10 1986-07-10 フロ−・サイトメ−タ−用のフロ−・チャンバ−装置
AT86904422T ATE43721T1 (de) 1985-07-10 1986-07-10 Durchflussvorrichtung fuer durchflusszytometer.
EP86904422A EP0229814B1 (en) 1985-07-10 1986-07-10 A flow chamber device for flow cytometers
DE8686904422T DE3663744D1 (en) 1985-07-10 1986-07-10 A flow chamber device for flow cytometers

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NO852761A NO156916C (no) 1985-07-10 1985-07-10 Stroemningskammer for vaeskestroemsfotometer.

Publications (3)

Publication Number Publication Date
NO852761L NO852761L (no) 1987-01-12
NO156916B true NO156916B (no) 1987-09-07
NO156916C NO156916C (no) 1987-12-16

Family

ID=19888388

Family Applications (1)

Application Number Title Priority Date Filing Date
NO852761A NO156916C (no) 1985-07-10 1985-07-10 Stroemningskammer for vaeskestroemsfotometer.

Country Status (7)

Country Link
US (1) US4737025A (no)
EP (1) EP0229814B1 (no)
JP (1) JPS62503192A (no)
AT (1) ATE43721T1 (no)
DE (1) DE3663744D1 (no)
NO (1) NO156916C (no)
WO (1) WO1987000282A1 (no)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63262565A (ja) * 1987-04-20 1988-10-28 Hitachi Ltd フロ−セル
DE69025370T2 (de) * 1989-10-04 1996-09-12 Canon Kk Gerät und Verfahren zur Trennung oder Messung von zu untersuchenden Teilchen in einer Flüssigkeitsprobe
US5275787A (en) * 1989-10-04 1994-01-04 Canon Kabushiki Kaisha Apparatus for separating or measuring particles to be examined in a sample fluid
NO894680L (no) * 1989-11-24 1991-05-27 Flowtech A S V Harald Steen Pulsmodulasjon av eksitasjonslyskilden i vaeskestroemcytofotometere.
DK111990D0 (da) * 1990-05-04 1990-05-04 Biometic Aps Apparat og fremgangsmaade til analyse af en vaeskesuspension
JP3232145B2 (ja) * 1991-12-27 2001-11-26 シスメックス株式会社 網赤血球測定方法
US5818731A (en) * 1995-08-29 1998-10-06 Mittal; Gauri S. Method and apparatus for measuring quality of frying/cooking oil/fat
US6819411B1 (en) 1997-01-31 2004-11-16 Xy, Inc. Optical apparatus
US5861950A (en) * 1997-07-10 1999-01-19 Particle Measuring Systems, Inc. Particle detection system utilizing an inviscid flow-producing nozzle
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US6263745B1 (en) * 1999-12-03 2001-07-24 Xy, Inc. Flow cytometer nozzle and flow cytometer sample handling methods
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
AU2002220018A1 (en) 2000-11-29 2002-06-11 Colorado State University System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
WO2004012837A2 (en) * 2002-08-01 2004-02-12 Xy, Inc. Low pressure sperm cell separation system
CN100570360C (zh) 2002-08-15 2009-12-16 Xy公司 一种流式细胞仪及流式细胞计数方法
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
JP4614947B2 (ja) 2003-03-28 2011-01-19 イングラン・リミテッド・ライアビリティ・カンパニー 粒子を仕分けるためおよび性別で仕分けられた動物の精子を提供するための装置、および方法
US20060263829A1 (en) * 2003-05-15 2006-11-23 Evans Kenneth M Efficient haploid cell sorting flow cytometer systems
DK2151243T3 (da) 2004-03-29 2013-01-28 Inguran Llc Sædcelleopslemninger til sortering i X- eller Y-kromosombærende berigede po-pulationer
EP2269617B1 (en) 2004-07-22 2016-04-27 Inguran, LLC Process for enriching a population of sperm cells
EP2232229B1 (en) 2007-12-04 2021-02-17 Particle Measuring Systems, Inc. Two-dimensional optical imaging methods for particle detection
US8589851B2 (en) * 2009-12-15 2013-11-19 Memoir Systems, Inc. Intelligent memory system compiler
US8614793B2 (en) * 2012-04-02 2013-12-24 Ecolab Usa Inc. Flow chamber for online fluorometer
US11668640B2 (en) 2015-03-06 2023-06-06 Inguran, Llc Nozzle assembly for a flow cytometry system and methods of manufacture
EP2898057B1 (en) 2012-09-19 2022-07-06 Inguran, LLC Flow cytometer system with a chamfered nozzle tip
US9027850B2 (en) 2012-09-19 2015-05-12 Inguran, Llc Nozzle assembly for a flow cytometer system and methods of manufacture
CN109295185B (zh) * 2018-09-05 2022-03-22 暨南大学 一种适用于单细胞真核藻类基因组大小的测定方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2709399C3 (de) * 1977-03-04 1980-07-24 Goehde, Wolfgang, Dr., 4400 Muenster Einrichtung zum Messen von Zelleigenschaften
DE2853703A1 (de) * 1978-12-13 1980-07-03 Leitz Ernst Gmbh Kuevette zur mikroskopischen beobachtung und/oder optisch- elektrischen messung von in einer fluessigkeit suspendierten teilchen
US4408877A (en) * 1979-04-10 1983-10-11 Ernst Leitz Wetzlar Gmbh Device for hydrodynamic focussing of a particle-suspension in a liquid flow cytophotometer
NO144002C (no) * 1979-04-10 1981-05-27 Norsk Hydro S Inst For Kreftfo Anordning for anvendelse ved vaeskestroemsfotometri
NO145176C (no) * 1979-11-23 1982-01-27 Norsk Hydros Inst For Kreftfor Anordning ved vaeskestroemsfotometer.

Also Published As

Publication number Publication date
JPS62503192A (ja) 1987-12-17
NO156916C (no) 1987-12-16
JPH0355781B2 (no) 1991-08-26
EP0229814A1 (en) 1987-07-29
NO852761L (no) 1987-01-12
EP0229814B1 (en) 1989-05-31
DE3663744D1 (en) 1989-07-06
US4737025A (en) 1988-04-12
WO1987000282A1 (en) 1987-01-15
ATE43721T1 (de) 1989-06-15

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