NO136370B - - Google Patents

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Info

Publication number: NO136370B
Authority: NO; Norway
Prior art keywords: enzymes; activity; enzyme; proteolytic; nutrient medium
Prior art date: 1967-10-03

Application number

NO145070A

Other languages

English (en)

Norwegian (no)

Other versions

NO136370C (no

Inventor

Knud Aunstrup

Otto Andresen

Helle Outtrup

Original Assignee

Novo Terapeutisk Labor As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1967-10-03

Filing date

1970-04-16

Publication date

1977-05-16

1968-07-26 Priority claimed from GB35921/68A external-priority patent/GB1243784A/en

1968-09-23 Priority claimed from NO3755/68A external-priority patent/NO136205C/no

1970-04-16 Application filed by Novo Terapeutisk Labor As filed Critical Novo Terapeutisk Labor As

1970-04-16 Priority to NO145070A priority Critical patent/NO136370C/no

1977-05-16 Publication of NO136370B publication Critical patent/NO136370B/no

1977-08-24 Publication of NO136370C publication Critical patent/NO136370C/no

Links

102000004190 Enzymes Human genes 0.000 claims description 49
108090000790 Enzymes Proteins 0.000 claims description 49
229940088598 enzyme Drugs 0.000 claims description 49
230000000694 effects Effects 0.000 claims description 24
239000003599 detergent Substances 0.000 claims description 22
102000035195 Peptidases Human genes 0.000 claims description 20
108091005804 Peptidases Proteins 0.000 claims description 20
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 claims description 18
238000000034 method Methods 0.000 claims description 17
230000002797 proteolythic effect Effects 0.000 claims description 16
235000015097 nutrients Nutrition 0.000 claims description 15
239000000203 mixture Substances 0.000 claims description 12
241000193830 Bacillus <bacterium> Species 0.000 claims description 10
241000894006 Bacteria Species 0.000 claims description 10
BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 9
229910001424 calcium ion Inorganic materials 0.000 claims description 9
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
239000004094 surface-active agent Substances 0.000 claims description 5
ROWKJAVDOGWPAT-UHFFFAOYSA-N Acetoin Chemical compound CC(O)C(C)=O ROWKJAVDOGWPAT-UHFFFAOYSA-N 0.000 claims description 4
229910052799 carbon Inorganic materials 0.000 claims description 4
229910052757 nitrogen Inorganic materials 0.000 claims description 4
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
241000193752 Bacillus circulans Species 0.000 claims description 2
241000193747 Bacillus firmus Species 0.000 claims description 2
241000194108 Bacillus licheniformis Species 0.000 claims description 2
241000194103 Bacillus pumilus Species 0.000 claims description 2
235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
125000000129 anionic group Chemical group 0.000 claims description 2
238000006243 chemical reaction Methods 0.000 claims description 2
GFAZHVHNLUBROE-UHFFFAOYSA-N hydroxymethyl propionaldehyde Natural products CCC(=O)CO GFAZHVHNLUBROE-UHFFFAOYSA-N 0.000 claims description 2
235000021184 main course Nutrition 0.000 claims description 2
238000012423 maintenance Methods 0.000 claims description 2
239000004615 ingredient Substances 0.000 claims 1
235000019833 protease Nutrition 0.000 claims 1
238000005406 washing Methods 0.000 description 23
238000012360 testing method Methods 0.000 description 20
239000002609 medium Substances 0.000 description 15
238000004140 cleaning Methods 0.000 description 13
238000002474 experimental method Methods 0.000 description 12
239000003795 chemical substances by application Substances 0.000 description 10
229940079919 digestives enzyme preparation Drugs 0.000 description 9
150000001720 carbohydrates Chemical class 0.000 description 7
235000014633 carbohydrates Nutrition 0.000 description 7
241000894007 species Species 0.000 description 7
238000005259 measurement Methods 0.000 description 6
239000000523 sample Substances 0.000 description 6
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
238000004458 analytical method Methods 0.000 description 5
230000000875 corresponding effect Effects 0.000 description 5
238000011835 investigation Methods 0.000 description 5
239000007788 liquid Substances 0.000 description 5
230000001105 regulatory effect Effects 0.000 description 5
150000001875 compounds Chemical class 0.000 description 4
238000004453 electron probe microanalysis Methods 0.000 description 4
UFZOPKFMKMAWLU-UHFFFAOYSA-N ethoxy(methyl)phosphinic acid Chemical compound CCOP(C)(O)=O UFZOPKFMKMAWLU-UHFFFAOYSA-N 0.000 description 4
239000001963 growth medium Substances 0.000 description 4
GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 4
239000000126 substance Substances 0.000 description 4
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
102000001554 Hemoglobins Human genes 0.000 description 3
108010054147 Hemoglobins Proteins 0.000 description 3
150000004649 carbonic acid derivatives Chemical class 0.000 description 3
239000011521 glass Substances 0.000 description 3
239000000843 powder Substances 0.000 description 3
239000000047 product Substances 0.000 description 3
235000002639 sodium chloride Nutrition 0.000 description 3
235000019832 sodium triphosphate Nutrition 0.000 description 3
UNXRWKVEANCORM-UHFFFAOYSA-I triphosphate(5-) Chemical compound [O-]P([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O UNXRWKVEANCORM-UHFFFAOYSA-I 0.000 description 3
241001624918 unidentified bacterium Species 0.000 description 3
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical group [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
229920002472 Starch Polymers 0.000 description 2
238000005273 aeration Methods 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
239000000872 buffer Substances 0.000 description 2
239000005018 casein Substances 0.000 description 2
239000012459 cleaning agent Substances 0.000 description 2
238000003776 cleavage reaction Methods 0.000 description 2
239000013068 control sample Substances 0.000 description 2
-1 cornmeal Chemical compound 0.000 description 2
210000003608 fece Anatomy 0.000 description 2
238000000855 fermentation Methods 0.000 description 2
230000004151 fermentation Effects 0.000 description 2
235000013312 flour Nutrition 0.000 description 2
239000010871 livestock manure Substances 0.000 description 2
244000005700 microbiome Species 0.000 description 2
235000013336 milk Nutrition 0.000 description 2
239000008267 milk Substances 0.000 description 2
210000004080 milk Anatomy 0.000 description 2
230000000877 morphologic effect Effects 0.000 description 2
YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
239000002244 precipitate Substances 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
230000007017 scission Effects 0.000 description 2
239000000344 soap Substances 0.000 description 2
229960001922 sodium perborate Drugs 0.000 description 2
235000010265 sodium sulphite Nutrition 0.000 description 2
YKLJGMBLPUQQOI-UHFFFAOYSA-M sodium;oxidooxy(oxo)borane Chemical compound [Na+].[O-]OB=O YKLJGMBLPUQQOI-UHFFFAOYSA-M 0.000 description 2
239000002689 soil Substances 0.000 description 2
239000007787 solid Substances 0.000 description 2
230000006641 stabilisation Effects 0.000 description 2
238000011105 stabilization Methods 0.000 description 2
235000019698 starch Nutrition 0.000 description 2
239000008107 starch Substances 0.000 description 2
238000003756 stirring Methods 0.000 description 2
238000003860 storage Methods 0.000 description 2
239000000271 synthetic detergent Substances 0.000 description 2
102000009027 Albumins Human genes 0.000 description 1
108010088751 Albumins Proteins 0.000 description 1
235000017060 Arachis glabrata Nutrition 0.000 description 1
244000105624 Arachis hypogaea Species 0.000 description 1
235000010777 Arachis hypogaea Nutrition 0.000 description 1
235000018262 Arachis monticola Nutrition 0.000 description 1
229910014813 CaC2 Inorganic materials 0.000 description 1
244000068988 Glycine max Species 0.000 description 1
235000010469 Glycine max Nutrition 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
239000005909 Kieselgur Substances 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
240000007594 Oryza sativa Species 0.000 description 1
235000007164 Oryza sativa Nutrition 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
229920000388 Polyphosphate Polymers 0.000 description 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
240000006394 Sorghum bicolor Species 0.000 description 1
235000011684 Sorghum saccharatum Nutrition 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
244000299461 Theobroma cacao Species 0.000 description 1
235000009470 Theobroma cacao Nutrition 0.000 description 1
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
240000008042 Zea mays Species 0.000 description 1
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
235000002017 Zea mays subsp mays Nutrition 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
229910052783 alkali metal Inorganic materials 0.000 description 1
150000001340 alkali metals Chemical class 0.000 description 1
125000002947 alkylene group Chemical group 0.000 description 1
102000004139 alpha-Amylases Human genes 0.000 description 1
108090000637 alpha-Amylases Proteins 0.000 description 1
229940024171 alpha-amylase Drugs 0.000 description 1
150000003863 ammonium salts Chemical class 0.000 description 1
239000003945 anionic surfactant Substances 0.000 description 1
238000003556 assay Methods 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
238000005842 biochemical reaction Methods 0.000 description 1
239000008280 blood Substances 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
150000001642 boronic acid derivatives Chemical class 0.000 description 1
239000001506 calcium phosphate Substances 0.000 description 1
235000011010 calcium phosphates Nutrition 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
239000000969 carrier Substances 0.000 description 1
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
235000021240 caseins Nutrition 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
235000013339 cereals Nutrition 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
239000003638 chemical reducing agent Substances 0.000 description 1
238000009833 condensation Methods 0.000 description 1
230000005494 condensation Effects 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
238000001816 cooling Methods 0.000 description 1
235000005822 corn Nutrition 0.000 description 1
235000012343 cottonseed oil Nutrition 0.000 description 1
239000013078 crystal Substances 0.000 description 1
239000008367 deionised water Substances 0.000 description 1
229910021641 deionized water Inorganic materials 0.000 description 1
238000011161 development Methods 0.000 description 1
239000008121 dextrose Substances 0.000 description 1
238000001035 drying Methods 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000001914 filtration Methods 0.000 description 1
230000003861 general physiology Effects 0.000 description 1
239000008103 glucose Substances 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
230000002779 inactivation Effects 0.000 description 1
229910017053 inorganic salt Inorganic materials 0.000 description 1
238000002955 isolation Methods 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
235000012054 meals Nutrition 0.000 description 1
238000010907 mechanical stirring Methods 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
230000004060 metabolic process Effects 0.000 description 1
150000002823 nitrates Chemical class 0.000 description 1
125000001477 organic nitrogen group Chemical group 0.000 description 1
125000001741 organic sulfur group Chemical class 0.000 description 1
235000020232 peanut Nutrition 0.000 description 1
235000021317 phosphate Nutrition 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
239000001205 polyphosphate Substances 0.000 description 1
235000011176 polyphosphates Nutrition 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
235000018102 proteins Nutrition 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
235000009566 rice Nutrition 0.000 description 1
238000000926 separation method Methods 0.000 description 1
229940071207 sesquicarbonate Drugs 0.000 description 1
150000004760 silicates Chemical class 0.000 description 1
239000000779 smoke Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910001467 sodium calcium phosphate Inorganic materials 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
229910052938 sodium sulfate Inorganic materials 0.000 description 1
235000011152 sodium sulphate Nutrition 0.000 description 1
238000013112 stability test Methods 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
230000000087 stabilizing effect Effects 0.000 description 1
229910001220 stainless steel Inorganic materials 0.000 description 1
239000010935 stainless steel Substances 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
235000000346 sugar Nutrition 0.000 description 1
150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
239000000725 suspension Substances 0.000 description 1
239000003760 tallow Substances 0.000 description 1
239000008399 tap water Substances 0.000 description 1
235000020679 tap water Nutrition 0.000 description 1
235000013619 trace mineral Nutrition 0.000 description 1
239000011573 trace mineral Substances 0.000 description 1
QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
238000005303 weighing Methods 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Landscapes

Detergent Compositions (AREA)
Enzymes And Modification Thereof (AREA)

NO145070A 1967-10-03 1970-04-16 Proteolytisk enzymholdige rense- og vaskemiddelblandinger. NO136370C (no)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
NO145070A NO136370C (no)	1967-10-03	1970-04-16	Proteolytisk enzymholdige rense- og vaskemiddelblandinger.

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
GB4504667		1967-10-03
GB35921/68A GB1243784A (en)	1967-10-03	1968-07-26	Proteolytic enzymes, their production and use
NO3755/68A NO136205C (no)	1967-10-03	1968-09-23	Fremgangsm}te for fremstilling av enzymer.
NO145070A NO136370C (no)	1967-10-03	1970-04-16	Proteolytisk enzymholdige rense- og vaskemiddelblandinger.

Publications (2)

Publication Number	Publication Date
NO136370B true NO136370B (zh)	1977-05-16
NO136370C NO136370C (no)	1977-08-24

Family

ID=27448867

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO145070A NO136370C (no)	1967-10-03	1970-04-16	Proteolytisk enzymholdige rense- og vaskemiddelblandinger.

Country Status (1)

Country	Link
NO (1)	NO136370C (zh)

1970
- 1970-04-16 NO NO145070A patent/NO136370C/no unknown

Also Published As

Publication number	Publication date
NO136370C (no)	1977-08-24

Publication	Publication Date	Title
US3723250A (en)	1973-03-27	Proteolytic enzymes, their production and use
Hammami et al.	2018	Proteolytic and amylolytic enzymes from a newly isolated Bacillus mojavensis SA: characterization and applications as laundry detergent additive and in leather processing
Bridson et al.	1970	Chapter III Design and formulation of microbial culture media
Letourneau et al.	1998	Keratinolytic activity of Streptomyces sp. S. K1–02: a new isolated strain
EP0290568B1 (en)	1991-03-13	Enzymatic detergent additive
US3907645A (en)	1975-09-23	Cholesterol assay
US5635468A (en)	1997-06-03	Liquefying alkaline α-amylase, process for producing the same, and detergent composition containing the same
US3623957A (en)	1971-11-30	Preparation of microbial alkaline protease by fermentation with bacillus subtilis, variety licheniformis
Fawole et al.	2003	Some factors affecting production of pectic enzymes by Aspergillus niger
US3674643A (en)	1972-07-04	Preparation of proteolytic enzymes having maximum activity at high alkalinity
JPH01273587A (ja)	1989-11-01	アルカリセルラーゼおよびその製造法
DK158233B (da)	1990-04-16	Proteasepraeparat, fremgangsmaade til fremstilling deraf og anvendelse deraf
Vosti et al.	1954	Autolysis of bakers' yeast
US5240851A (en)	1993-08-31	Lipase-producing Pseudomonas aeruginosa strain
Guilloux-Benatier et al.	2000	Lysis of yeast cells by Oenococcus oeni enzymes
US5100796A (en)	1992-03-31	Methods for producing a new pseudomonas lipase and protease and detergent washing compositions containing same
Poincelot et al.	1972	Simple dye release assay for determining cellulolytic activity of fungi
Jönsson et al.	1964	Protease production by Aspergillus fumigatus
US3960665A (en)	1976-06-01	Production of proteolytic enzymes
KR100237969B1 (ko)	2000-01-15	신규 프로테아제
Ozdenefe et al.	2017	Optimization of culture conditions for alkaline protease production from waste breads using Bacillus subtilis
NO136370B (zh)	1977-05-16
KR100249995B1 (ko)	2000-03-15	터모코쿠스 유래 열안정성 프로테아제
Kilpatrick et al.	1977	Glycosidases from the culture medium of Physarum polycephalum
DE2925427C2 (de)	1983-12-22	Verfahren zur Herstellung von Protease