NL8202885A - PREPARATIONS WITH AN EFFECT ON VIRUSES AND METHODS AND PRODUCTS USING SUCH PREPARATIONS. - Google Patents

Description

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Anti-virus preparations and methods and products using such preparations.

The invention relates to a class of compositions having an activity against viruses which are very effective against common respiratory viruses, such as rhinoviruses, parainfluenza viruses and adenoviruses, and to methods and products using such preparations. The invention particularly relates to a new type of virucidal composition which can be applied to various substrates such as cellulosic webs, non-woven structures and textile-based materials. In addition, the group of virucidal compositions to which this invention pertains may also be included in nasal sprays, facial creams, hand lotions, lipsticks and corresponding cosmetic preparations. The formulations can also be used as ingredients in kitchen and bathroom cleaners, furniture and floor polishes and similar household formulations.

Virologists, recognized in the field of respiratory viruses, generally agree that rhinoviruses, influenza viruses and adenoviruses are among the major group of pathogens that cause respiratory diseases. In particular, rhinoviruses are considered the major causative agent of what is generally known as ordinary cold.

The term "rhinovirus reflects the development of the nose leading to excessive nasal discharges when infections are caused by this group of viruses. Bhinoviruses belong to the picornavirus family of viruses which, in the absence of an external envelope, are often characterized as naked viruses Although more than 100 different antigenic types of rhinoviruses are known, they share common, important general 3202885 ♦ - 2 properties, for example, they have all ether resistant capsids and all contain single stranded ENA (about 2.6 x 10 daltons) Furthermore, they are all difficult to inactivate by common germicides, such as quaternary ammonium compounds.

Adenoviruses include more than thirty antigenic types. If they enter the respiratory system they cause inflammation of the tissues leading to symptoms of pharyngitis, bronchitis, etc. Although most adenovirus infections occur in childhood, adult infections are far from uncommon. Like rhinoviruses, adenoviruses lack a membrane shell, but the adeno cores, in contrast to the rhino cores, contain a double stranded DM. Adenoviruses are extremely resistant to inactivation. Parainfluenza viruses belong to the paramyxovirus family. They play an important role in the prevention of diseases of the lower respiratory tract in children and of the upper respiratory tract in adults. The parainfluenza viruses are MA-containing viruses with an ether sensitive lypo-20 protein envelope surrounding the nucleocapsid. These viruses are resistant to inactivation due to low concentrations of carboxylic acids.

Recent work by Dick et al. / Dick, E.C. and Chesney, PJ, "Textbook of Pediatric Diseases, Feigin, RD and 25 Cherry, JD ed., Vol. II, biz. 1167 (19δ1) WB Saunders Pub. Co., Phila., PA / has shed much light on the Mode of transmission of respiratory diseases caused by rhinoviruses Although the exact mode of transmission of respiratory diseases is not fully known, field studies by the aforementioned researchers have convincingly shown that the effective transmission of diseases, such as ordinary cold, usually a close approach or contact, directly or indirectly, of the infected object and the potential sacrificial sacrifice required (indirect contact can be understood as a contact which takes place via an intervening surface, for example 8202885 5 * - 3 - table top or doorknob etc.) It will thus be possible to chain to stop infection and reduce its ability to spread if the viruses are rendered ineffective by immediate exposure to a virucidal agent 5 as soon as they are delivered through the nose of an infected person. In addition, upon manifestation, viruses hiding in the face or hands of an infected person may also be killed if an appropriate germicidal agent is quickly brought into contact with the appropriate surface of the body, that is, face, hands, etc. A facial tissue or cloth containing a fast-acting, effective virucidal preparation is a suitable means of attaining the aforementioned objectives.

There has long been a need for a safe and inexpensive virucidal agent effective against the respiratory or respiratory tract viruses. Simple household germicides are not effective against rhino and adenoviruses.

United States Patent No. 0-5-36 ^ 4 in the name of Richter discloses a wipeable paper product impregnated with an iodophore (that is, iodine and a carrier) having germicidal properties, which is useful as a pruning agent in a surgical routine vas treatment. In this patent it has been noted that the stability of the iodophor is increased at a low pH and that small amounts of such organic acids, such as citric or acetic acid, can be added to control the pH. U.S. Patent 3,881,210 to Drach and co-distributors discloses a pre-wetted sanitary napkin wipe that may contain a bactericide. US Pat. No. 3,651,165 to Bryant and co-publishers discloses a detergent-hygienic take-off agent with iodine having a bactericidal action. Previously, Shepherd et al., U.S. Pat. No. 8202885, U.S. Pat. No. 3,567,118, refers to a cleaning fiber material with a sheath of a hydrophilic aerylate or methacrylate containing, inter alia, a bactericide.

Although it has been described in the prior art that iodine preparations and products have a virucidal effect with a wide spectrum, an inexpensive product which successfully combats the spread of viruses such as rhinovirus or influenza virus has yet to be developed commercially.

For example, difficulties with iodine result from its toxicity and the fact that it is a primary irritant to animal tissue. This action is non-selective for the proteins of bacteria or mammals and its undose use on the skin can cause ulcers.

In addition, its activity can be reduced or counteracted by the action of biological fluids, such as blood serum. Attempts to modify iodine to avoid these difficulties have not been entirely successful.

There are references in the literature to the bac-20 tericiae action of acids, such as citric acid, for example,

Beid, James D., "The Disinfectant Action of Certain Organic Acids," American Journal of Hygiene, 16, 5 ^ 0-556 (1932) J. However, the virucidal activity differs fundamentally from bactericidal activity, because viruses and bacteria are different microorganisms with different properties. For example, viruses do not replicate outside host cells and bacteria. Quaternary ammonium compounds, such as benzalkonium chloride, are often effective against bacteria, but not against viruses, such as the various rhinoviruses.

Although rhinoviruses are known to be unstable to aqueous solutions of acids under low pH conditions / eg Davis, B.D. and employees; "Microbiology" biz. 1303. Harper E. Row (publishers) New York, 1973 and Rueckert, R.R., "Picornaviral Architecture" Comparative 35 Virology- Academic Press, New York (1971) 9 biz. 19 ^ -3067, 8202885 J 5 - 5 - known references do not mention the application of this concept in an epidemiological context, such as interruption of the chain of infection caused by rhinoviruses. The only systematic study of the virucidal activity of organic 5-znren (citric, malic, etc.) that appears in the widely available literature was conducted by Poll, Biondi, Uberti, Ponti, Balsari and Cantoni / Poll, G. and collaborators: " Yirucidal Activity of Organic Acids "Food Chem. (England) k (k) 251 ~ 8 (1979) 7 · These researchers found, inter alia, that citric acid, malic acid, pyruvic acid and succinic acid are effective against herpes viruses, orthomyxoviruses and rhabdoviruses (Rabies virus). Their tests were performed at room temperature with aqueous solutions of the pure acids. No substrate or support was used. The three viruses selected by these 15 researchers for the study were all membrane-enveloped viruses, which in this respect resemble parainfluenza 3. Poli and co-workers also observed that these acids are not effective against adenovirus, which was previously reported noted, is a naked virus. On this basis, it was concluded that these acids are effective against membrane-enveloped viruses, but not against the naked viruses.

Experts know that adenoviruses are resistant to acids.

The present invention provides a virucidal product, preparation and method which are highly effective for a wide spectrum of viruses and yet can be prepared and used without any problem. The foregoing is based on the discovery that carboxylic acids, such as citric, malic and succinic acids, in a suitable carrier, are effective not only against certain membrane-enveloped respiratory viruses, but also against rhinovirus, a naked virus. In addition, in the presence of a surfactant, such as sodium dodecyl sulfate, they are also active against adenovirus. In addition, the products of the invention may also include a substrate, such as a facial tissue or a nonwoven web, in which such compositions are incorporated. According to the invention, an effective amount of such a composition is contacted (via these products) with the affected area. In general, these preparations can be handled without difficulty and do not lead to harmful effects when used according to the invention. In addition, the formulations applied to a substrate, such as a fabric intended for the face, have no or little harmful effects on color, odor, strength or other important properties. The products can be used as a dry wiping cloth, but they can also be kept moist and used as, for example, a damp wiping cloth.

The present invention is the result of the surprising finding that certain acids, such as citric, malic, succinic and benzoic acids, used in appropriate concentrations and as described further below, are very effective against rhinoviruses 16, 1A and 86 and 20 parainfluenza 3. Applied in the presence of a surfactant, such as sodium dodecyl sulfate (SDS), these acids are also found to be effective against adenovirus 5 · (The special viruses selected for study, i.e. RV-16, RV -IA, RV-86, Para-3, and Adeno-5, are representative of their 25 classes). Generally, the carboxylic acids which can be used in the invention will have formula R-C00H, where R is a lower alkyl group (1-6 carbon atoms), a substituted lower alkyl group, / e.g. a hydroxyl-substituted lower alkyl group (e.g. CH 2 CHOH- ), a carboxyl-substituted lower alkyl group (e.g., H00C-CH -CH-), a carboxyl and hydroxyl-substituted lower alkyl group (e.g., EOOCCH ^ CROH-), a carboxyl and halogen-substituted lower alkyl group (e.g., H00CCS CHBr-), a lower alkyl group substituted by carboxyl and two hydroxyl groups (for example H00C-CH0H-CH0H -) - a lower alkyl group substituted by two carboxyl 8202885-7 and hydroxyl,

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(e.g. HOOC-CH ^ C-CH ^ -).

ίίΟΟΗ 5 a low alkenyl group, a carboxyl-substituted low alkenyl group (for example, H00CCH = CH-), a low alkenyl group substituted by two carboxyl groups

COOH

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(for example, H00C-CH2 C = CH-), a phenyl group, a substituted phenyl group (for example, a hydroxyl-substituted phenyl group HO-C8H2-), etc. can be. The above definitions are for illustrative purposes only and are not limitative.

The surfactant may be nonionic (for example, the polyoxyethylenated alkyl phenols, such as TRITON X-100 from Rohm and Haas, the polyoxyethylated sorbitol esters such as TWEEN W ^ from ICI United States, Inc.), cationic (e.g., cetylpyridinium chloride. (C ^ H 2 H 2 CH 2 Cl 2 Cl and methylbenzethonium chloride

Me h 25 (Me3CCH2C (Me) 2-C6H3 (Me) -OCH2CH2OCH2-CHj-CH2C6H5 Cl)

Me or anionic (for example, sodium dodecyl sulfate (CH 2 CB 2) CH 2 SO 3 -Na), the 1.1 + bis (2-ethylhexyl) ester and sodium salt of sulfosuccinic acid, from the American Cyanamid Company under the trade name AEROSOL 0TV The recommended anionic surfactant means can be represented by formulas 35 1. (R0S0) M +

j X

8202885-8 where Μ is a monovalent, bivalent or trivalent metal cation or an ammonium or substituted ammonium ion, x an integer and R represents an alkyl group.

f jWl \ 5

\ 03S-CEC02E2 j X

where M + and x have the meanings defined above 10 · .... . . .

and R 1 and R 1, which may be all or the same, may represent a straight or branched chain aliphatic group. Generally, surfactants used alone are not virucidal to the naked viruses, such as rhinovirus.

1C.

J Although the invention is limited to the use of a cellulose fabric (such as a fabric for facial treatment, bathroom fabrics, towels, etc.) as the substrate or carrier for virucidal agents,

with a tissue intended for treatment of the face geimpreg-PO

These novel virucidal agents have sufficiently explained the basic principle of the invention and this constitutes a simple and useful embodiment of the invention. Therefore, the following studies were performed with tissues intended for treatment of the face as a substrate. Examples of suitable nonwoven substrates include wet wiping materials such as damp crepe towels and spunbond and meltblown polymeric fabrics commonly used in the manufacture of hospital materials such as surgical clothing, pillow cases, sheets, cloaks, etc. are applied. All kinds of textile materials, including laminates of different materials, can also be used as suitable substrates. For example, hygienic face masks for persons suffering from respiratory diseases are excellent examples where the present invention can be used. Other carriers 8202885-9 will be apparent to those skilled in the art and may, for example, be intended as lotions, sprays, creams, cleansers, etc.

Generally speaking, the method of preparing or manufacturing the samples used in the following examples was simple and proceeded without complications. Three folded KLEENE facial towels (27.5 x 30.0 cm, 2 basis weight about 26 lb / 2880 ft. For all three folded parts together) were impregnated by simple immersion with aqueous solutions of citric acid, malic acid, succinic acid and benzoic acid. The acids were used either individually or as a homogeneous mixture. Typically, the impregnating solution also contained a small percentage of a surfactant, such as Aerosol-OT- / sodium salt of the 1, Wbis (2-ethylhexyl) ester of sulfosuccinic acid, of

American Cyanamid /, or sodium dodecyl sulfate. A small amount of glycerol was also used to improve the softness of the fabric. The saturated fabrics were pressed between rolls to remove excess liquid and ensure even saturation. The fabrics were weighed, dried and the degree of saturation (i.e. the percentage incorporated for saturation) was calculated. The tissues were then ready for virucidal activity studies.

The method used to investigate the virucidal activity was according to standard virologic investigation methods (TCIDrn) with simple variations required by the presence of the cellulose substrate.

30 Here is a description of the method used:

Virucidal Test Method I. Materials: A. Solutions: 1. Neutralize Solution ml 2 M Na ^ HPO ^ 35 8202885 -ΙΟΙ, 2 ml 1.0 Μ Citric Acid 92.4 ml 1X Medium 199 (tissue culture nutrient medium) 5 2. Hank's - Mcllvaine Saline (HMSS): 2.0 ml of 1.0 citric acid diluted to 2 L with the equilibrium saline of Hank 18.0 ml of 2.0 M sterile Ha.HPO,.

2 4 the pH of this solution is 7.0 10 3. Hank's equilibrium salt solution: (g / l in twice distilled water) HaCl 8.0 KC1 0.4 15 MgS0 ^ .7H20 0.2

CaClg (anhydrous) 0.14

KagHPO4 .aHgO 0.06 KH2P ° 4 (anhydrous) 0.06

Glucose 1.0 20 Phenol Red 0.005

NaHCO3 0.35

Note: The solutions described here are not virucidal.

25 B. Viruses and tissue culture cells: 1. Rhinovirus type 16, type 1A and type 86? Rhinovirus types 16, 1A and 86 (RV-16, 1A and 86, respectively) were cultured in Ohio State HeLa (0-HeLa) tissue culture cells and stored at -51 ° C until use. The virucidal examination with the rhinoviruses was performed using 0-HeLa tissue culture test tubes incubated in a revolving drum at 33 ° C.

35 8202885 -11-

t I

2, Parainfluenza type 3: Parainfluenza type 3 (para 3) was grown in culture cells of rhesus monkey kidney tissue and stored at -51 ° C until use. The virueidal assay with Para 3 virus was performed using 5-HeLa tissue culture test tubes incubated stationary at 33 ° C.

3. Adenovirus type 5. Adenovirus type 5 (Adeno 5) was grown in culture cells of HEp-2 tissue and stored at -51 ° C until use. The virueidal study with adeno 5 virus was performed using Human Epitheleal Carcinoma - 2 (HEp-2) tissue culture test tubes incubated at 3 ° C in a stationary state.

15 II. Methods.

A. Virucidal research:

A mixture of equal parts by volume of virus and saliva was prepared. From a Kimberly-Clark KLEENE fabric 2. .

a sample of 6.25 cm was cut and brought into a Petn-20 plastic dish (a treated fabric is a fabric impregnated with the virueidal agent to be examined) ,, The mixture of virus and saliva (0.1 ml ) was pipetted directly onto the sample and allowed to act for 1 minute. It is noted that this is a two-fold virus dilution. After 1 minute, 5 ml of neutralizing solution was pipetted onto the sample. the Petri dish and stirred for 3 seconds. This is now a hundred-fold virus dilution. The neutralizing solution, virus and saliva mixture was then pipetted from the Petri dish and added to a tube containing 5 ml of Hank's-30 Mcllvaine saline. The sample was now placed in the same tube by tapping the scale and pushing the sample into the tube with the tip of the pipette. The tube contained the 10 ml solutions and the sample was spun for 30 seconds. -2 -2

This tube contained a 5 or 1: 200 dilution of the virus.

-2 3 35 Of the 10 'dilution, a series of tenfold dilutions 8202885 -12- (with a fresh pipette for each dilution) was prepared by taking 0.3 ml of the previous dilution and adding it to 2.7 ml of the Hanks' - Mcllcaine saline. 0.1 ml was seeded in each test tube of the tissue culture. Generally, two tubes per dilution were grafted.

Two sets of controls were used for each study. The first can be termed "the virus control", since it aims to control the infectivity of the virus suspension itself, without saliva or tissue substrate. The virus suspension was diluted in a 10-fold series in HMSS. 0.1 ml of the specific dilutions were seeded per tissue culture cell test tube. The information obtained from this control indicated the number of infectious virus units present in the virus solution stored at -51 ° C and ensures that a proportional portion of the virus solution used in the experiment during freezing, storing or thawing had not lost any of its infectivity.

The second control, "the tissue control," was based on performing a virucidal assay using 6.25 cm of untreated KLEENE tissue. The information obtained from this control indicated the number of infectious virus units that can be recovered from an untreated 6.25 cm wipe using the virucidal assay method. The grafted tissue culture tubes were observed for 7 days to demonstrate viral infection.

The end point of a virucidal assay for a given wipe is that dilution of the virus infecting only one of the two inoculated tubes. This number is defined as the tissue culture infection dose or TCID. The results of the virucidal action of a given wipe are usually expressed as the "log difference" between the ordinary log of the TCID result of the treated sample, subtracted from the ordinary log of the TCID of the untreated 35. sample.

8202885 Γ -13-

The virueidal activity of a sample can be derived from the "log difference" in the following way: Λ-Μ 5 Virucidal distortion = / - 100% \ x 1 in X = the initial concentration of the virus (infectious 10 units / 0.1 ml) of the untreated sample used as control.

Y = the final concentration of the virus (infectious units / 0.1 ml) of the treated sample.

In the following examples the calculation method is illustrated (in these tests the final virus concentration is always equal to or less than 10 3 infectious units / 0.1 ml).

For the majority of the results obtained, 2 3 the virus final concentration is less than 10 *, with a virus 6 3 20 initial concentration of 10 3, this indicates a log difference greater than U and a percent kill greater than 99 »99%) · 6 3 1. Initial concentration: X = 10 ° '2 3

Final concentration: Y = 10 3 25 Log difference = (log 10 ^> 3 - log 102’3) = i | / 106'3 - 102'3 \

Virucidal Efficiency = (- -7—5- / x 100% V 10 3 ^ y fo2,3 (io ^ ~ 1) \ _ = (-z-z- x 100%

30 V 10β, 3 J

= 99.99% 1+ 8 2. Initial concentration: X = 10 3 35 8202885 -1U-2 3

Final concentration: Y = 10 "

Log difference = 2.5 / M _ 2> 3 \

Virucidal Return = (-; - 5- x 100 $

\ J J

c = 99.7%?

The method described above is in accordance with standard microbiological research methods. Thereby reliable and reproducible results are obtained within the limits of variation that are bound to biological tests.

Results.

The results are shown in Tables A, B, and C. The data in Table A shows that simple organic carboxylic acids, such as citric, malic, tartaric, succinic, and derivatives thereof (e.g., 2-bromo-

M

succinic acid) and benzoic acid and its derivatives (salicylic acid) used in a tissue intended for the net face in suitable concentrations exert a potent virucidal action against rhinovirus 16 and parainfluenza 3.

In addition, when used in conjunction with a surfactant such as Aerosol 0T or sodium dodecyl sulfate, it has been found that the concentrations of the acids in the tissue destined for the face can be reduced without sacrificing the virucidal activity. .

Table B shows the results of tests with * * * acid mixtures selected from the group of citric, benzoic, succinic and malic acids. These data show that facial tissues treated with the acid-3 mixtures exert a virucidal action against rhinovirus 16 and parainfluenza 3. The data contained in Table B shows that a fabric for the face impregnated with a system of mixed acids, such as citric acid and malic acid, and a suitable surfactant, such as SDS, is effective against rhinovirus 16, 1A and 8202385-15. 86 and adenovirus 5 These examples demonstrate, in accordance with the invention, that simple organic acids, such as citric, malic, succinic acid, used in conjunction with a suitable surfactant, such as SDS, have potent virucidal activity against common respiratory or respiratory viruses, of which rhino virus 16, 1A and 86, parainfluenza 3 and adenovirus 5 are typical examples. In addition, products using the tissues intended for the face as a means of using the said preparations with virucidal activity are very effective.

The significance of the invention is based on the fact that it provides the base for interrupting the chain of infection caused by respiratory or respiratory viruses. Since viruses do not proliferate outside the host cell, the degree of inactivation demonstrated in the tests provides a simple and practical method for reducing the virus concentration in a person infected with a respiratory or respiratory infection. In turn, this spring will significantly reduce the ability of the infection to spread.

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In particular, in order to illustrate the improved effects obtained in accordance with the invention, additional examples were carried out in which the concentration of selected acid preparations were ionized and the virucidal activity was measured at 1 and 5 minutes. These results are summarized in Table D. Generally, the acid compositions of the invention are highly virueidally active, for example, in the case of rhinoviruses or parainfluenza, they bring a log drop of 2 or greater inactivation in 1 minute or 10 shorter time. For adenoviruses, the time will be 5 minutes or less. In general, the degree of inactivation after 5 minutes is greater than after 1 minute, as expected. Certain minor inconsistencies occur in the reported results due to the error limit and the manner in which the study was conducted. It will be appreciated by those skilled in the art that effectiveness is also affected by the amount of composition available for contact with the virus, which again depends on the nature of the carrier. For example, as will be seen from Table D below, a relatively thick carrier with large voids, such as wool, may be ineffective unless treated with large amounts of the composition. On the other hand, a lightweight, relatively closed structure, such as a fabric or nonwoven material, will require less preparation. Based on the tests described, the effectiveness of a certain combination of preparation and carrier can be determined.

As shown, for example, in Table D, citric acid has been effectively investigated in concentrations of 5-10% addition. The method used is described below.

For these examples TCID results were obtained using WI-38 cells from Flow Laboratories, Inc. which have only passed through a few passes and which have been passed through at least one pass in the beginning to ensure that they have growth potential. The contents of the flasks were then split 1: 2 and seeded into 96 well tissue plates with a 96-well assembly and a flat bottom growth area of 0.32 cm obtained from M A Bioproducts. The cells were incubated in 5% CO 2 at 37 ° C and after 2b hours before using them in the test were usually 80-90% coated with a web and had a normal appearance. The medium (2% MM) used for both the dilutions and cell maintenance was MEM Eagles with Earles BSS (with glutamine, gentamycin and 2% added fetal calf serum). The rhinovirus 1A was obtained from the National Institute of Allergy and Infectious Diseases,

Bethesda, Maryland. A vial was taken up by WI-38 cells and taken out again after a cytopathogenic effect (CPE) was demonstrated after 2 days post-inoculation. The virus was collected, aliquoted and frozen at -70 ° C and later titered a 15 in WI-38 cells in 96-well fused plate.

For the test, the medium was removed from the plates by inserting a sterile gauze between the plate and the lid and inverting the plate. All six 20 cups used received 0.1 ml 2% MM. To the wells used as cell controls, 0.1 ml 2% MM was added. To the cells to which the compounds were to be added, 0.1 ml of the appropriate dilution of the material was added to each of the six cups. For the initial 25 dilution, the virus stock was mixed 1: 1 with 2% MM. Then 100 microml of this virus dilution was added to a treated disk in a Petri dish. The virus was applied evenly over a tissue disc using a microliter syringe. The virus was left on the disk for 1 minute or 5 minutes, then 5 ml of 2% MM was added to the disk in the Petri dish and the disks / few moved. The disk and solution were removed and placed in a sterile tube and agitated for 30 seconds to give the first solution. From the original tube, 3 tenfold dilutions 8202835-21 were prepared and 0.1 ml of all four dilutions were added to the one-layer 1-38 cells. Six cups were used for each dilution. at 1 and 5 minutes, untreated controls were examined, with and without virus, and virus titration was also performed in each run. The plates were incubated again in 5% CO at 37 ° C for the duration of the run.

Acids such as sulfamic acid and phosphoric acid have also been found to have a virucidal effect. However, these acids have been found to decompose carriers, such as tissues.

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Since these acids are water soluble, they can be applied to many substrates with great ease from an aqueous solution such as by dipping, coating or other conventional methods such as spraying or gravure printing. When applied to the substrates, the composition is used in an amount sufficient to achieve a virucidal activity defined herein. Although the effective lower limit for the acids has not been accurately determined, generally for a material such as a fabric destined for the face with a basis weight between 23-31 lbs.: 2880 ft (3 x folded), the recording will be at least about 2%, and preferably about 5%, of acetic acids, such as citric acid, on a dry basis. Other substrates, such as nonwoven materials, can also be used.

As for the other substrates, those with high wettability are preferred. As already mentioned, the low virucidal activity in substrates such as wool is believed to be due to the inability of the virucidal composition to penetrate the substrate.

If mixtures of acids are used, they can be present in any amount, but preferably the mixtures contain at least about 0.2-10% of each acid, based on the substrate after drying.

If surfactants are also used, they are preferably selected from the group of anionic surfactants and included in an amount of about 0.05-5%, based on the weight of the substrate after drying.

When using the virucidally active organic acids, as defined above, in other substrates of carriers, such as lotions, mouthwashes, creams, sprays, cleansers, etc., the virucidally effective amount can be readily used by the means mentioned herein methods are determined. For example, a 35 log drop of 2 or more means that 99% or more of the host viruses 8202835 -26- are inactivated upon contact with the virus-inhibited acid preparations described herein.

Thus, according to the invention, a virucidal product is provided which, under the conditions of normal use, fully meets the objectives and has the advantages as stated in the foregoing description.

10 8202885

Claims (23)

A virucidal preparation, characterized in that it is a physiologically acceptable carrier containing a virucidally effective amount of one or more acids, each having formula R-COOH, wherein R is a lower alkyl group, a substituted lower alkyl group, a carboxyl group containing lower alkyl group, a carboxyl and hydroxyl containing lower alkyl group, a carboxyl and halogen containing lower alkyl group, a carboxyl and two hydroxyl groups containing lower alkyl group, a two carboxyl and hydroxyl containing lower alkyl group, a lower alkenyl group, a carboxyl group containing lower alkenyl - group, represents a lower alkenyl group containing two carboxyl groups, a phenyl group or a substituted phenyl group. Virucidal preparation according to claim 1, characterized in that it also contains a non-ionic, cationic and / or anionic surfactant. 3. A composition according to any one of the preceding claims, characterized in that the acid used is citric acid, malic acid, succinic acid, benzoic acid and / or a substituted derivative thereof, or a mixture of these acids. Preparation according to Claim 2 or 3, characterized in that the surface-active agent used is a polyoxyethylenated alkyl phenol, polyoxyethylenated sorbitol ester, quaternary ammonium salt, salt of an ester of sulfuric acid, salt of an alkyl sulfonic acid salt of a sulfosuccinic acid ester of formula f? V ° 2ri \ (eso3) m +? or (ROSO3] M + “Af M + LII 30 v 2 2y x” where M + represents a monovalent, divalent or trivalent metal cation or an ammonium or substituted ammonium ion, x is an integer 8202885 -28 number, R represents an alkyl group and R. and R., which may be the same or different, represents a straight or branched chain aliphatic group. 5. A composition according to claim h, characterized in that the surface active agent used is the sodium salt of the 1,1-bis (2-ethylhexyl) ester of sulfosuccinic acid and / or sodium dodecyl sulfate. 6. A composition according to any one of the preceding claims, characterized in that the acid chosen is a mixture of citric acid and malic acid. Preparation according to claims 1 to 5, characterized in that the acid selected is a mixture of citric acid and benzoic acid. 8. Preparation according to claims 1-5, characterized in that the acid chosen is a mixture of citric acid and succinic acid. Preparation according to claims 1 to 5, characterized in that the acid chosen is a mixture of malic acid and benzoic acid. 10. Preparation according to claims 1-5, characterized in that the acid chosen is a mixture of malic acid and succinic acid. 11. Preparation according to claims 1-5s, characterized in that the acid chosen is a mixture of succinic acid and benzoic acid. 12. A virucidal product incorporating a tissue or fly substrate containing an effective amount of a composition. -including one or more acids as defined in claim 1. Product according to claim 12, characterized in that this preparation also contains a non-ionic, cationic and / or anionic surfactant. 30 1k. Product according to claim 12 or 13, characterized in that a cellulose fabric, non-woven fabric and / or a textile material is used as the substrate. Product according to claim 1U, characterized in that the acid used is citric acid, succinic acid, benzoic acid and / or a substituted derivative thereof, or mixtures of these acids, the acid (s) being in a amount of about 2% or more of the surface of the substrate is present. 16. Product according to claim 1U, characterized in that as substrate a cellulose fabric is used which is suitable for the face. Product according to claim 1, characterized in that as the surfactant an agent as defined in claim 1 is used. 18. A product according to claim 1, characterized in that the surface-active agent used is the sodium salt of a 1,1-bis (2-ethylhexyl) ester of sulfosuccinic acid and / or sodium dodecyl sulfate. 19. A product according to claim 1, characterized in that the acid used is a mixture of citric acid and malic acid. The product according to claim 1U, characterized in that the acid used is a mixture of citric acid and benzoic acid. 21. A product according to claim 11, characterized in that the acid used is a mixture of citric acid and succinic acid. A product according to claim 11, characterized in that the acid used is a mixture of malic acid and benzoic acid. 23. A product according to claim 11, characterized in that the mixture used is an acid of a mixture of malic acid and succinic acid. 2k. Product according to claim 1H, characterized in that the acid used is a mixture of succinic acid and benzoic acid. A method of interrupting or preventing the spread of a respiratory virus, characterized in that an area in which a virus is present is treated with an effective amount of a preparation or product according to any one of the preceding claims. Products containing a composition according to claim 1 to 8202885 -30-5. Shaped products according to claim 26. / i -7 /, 11 '/ y / l' / '8202885